Spo25629 (gene) | SpinachBase

Overview


Name	Spo25629
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Tonoplast intrinsic protein 41
Location	chr1 : 38204273 .. 38206326 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCCAACTAACTAACTAGGATGACTGGATGAGTAGGTCATAAAAGGAGGAATATCCATAGAGGGTGGCATAAAGCAACAAAATAATTTTGTCATACAATACAGTACGTACGCCAATGATAATGATGAATTATCATTTTGATTTTTGACCCATCCATCTAATCATGTAATAACATGGACAAAGGAATCTCCACCTTTCCGGTTGTTTCACCCTTCAATTCATTGCTCTTTCTCATTTTAGTATTTATAAATGCACGTTTTTTAACTATATATATATATATATATATAAAAGTGATTTGCACCTCTGATAATTTGTTAACNGCTCTTTCTCATTTTAGTATTTATAAATGCACGTTTTTTAACTATATATATATATATATATATATATAAAAGTGATTTGCACCTCTGATAATTTGTTAACACTATACTCCATATCATCATGGGTAAAATAGCCCTAGGAACAACCCGAGAGGCAACGGAACCAGAATGCATCAATGCTCTCGTTGTCGAATTCATTTGCACCTTCCTCTTCGTCTTCGCCGGTGTTGGATCTGCCATGGCTGCTGGTAATTAACCACTCGATCAAATTTAACAATTTATATACTATGTCGTATTCCTATTATTAAGAATGATCGATTTAATTTTGTTTGTAATTGTAAAATGTTACAGATAAAGCAGAAGCAAGTCCAATAGTAGGATTGTTGTTTGTGGGGATAACACATGCATTTGTGGTAGCTGCGATGATTGCTGCCGGATTTAACATTTCCGGCGGTCACTTGAACCCCGCCGTCACTCTTGGTCTTGCAATTGGTGGCAATATTACAATTCTCAGATCTCTACTTTATTGGATTATACAATGTCTTGCTTCTGCTCTGGCTTGTTACTTGCTCACCTATGTCACCAGTGGACTGGTAAAATTTTATTTTTTACCTCATTTACTATTCCGAGATATATTATTCCTTGTCTACATTAAAATAAGATTAAATTATTTTTTACCATCAAATAAATTGATAAATTGTTTTTTACCGCCTAAAAAAATAAAAAGAGTCTTACCACCTAAATATAAAATAAAAATTATTTTTTACCACCTCTTAACCGGAAAAATTGAAAGAAATGTTATGCGGATCCATTTCAACGACTATATTTTTCATTTTCTTATATTCTCCCATGATTTTCATGTATGTAAGCTACTATTTTCCTTTACTTTCCATTAATTCACATACCATTTTTGTCCATCTTGTAGTTAAGAGGTGGTAAAAAATAATTTTATTTTATTTTTAGGTAGTAAAAGACAATTTTTATTTTTTTAGGTCATAAAAACAATTTATCAAATTTTTTAAGTGATAAAAAACAATTTAACCTTAAACTAATTAGTCACTCCGTCTAAATTTAGTGCGTTCTATTTACCTAATTAAGTGATTATCACTTATCTGAACGCAGGTGACTCCTATACACTCTCTTCCAAAGGGGGTAGACCCTCTACAAGGATTGGTGATGGAGGTGATCTTAACATTTTCTCTCCTCTTCACAATATACACCACCCTTGTTGACCAAAAGAAGGGCCCACTTCAAAGCCAAGGAATATTGCTAACTGGGCTTGTGGTTGGGGCCAACATCTTTGCTGGTGGGTTATTCTCTGGGGCATCAATGAATCCAGCAAGGTCATTTGGGCCTGCTCTTGTAAGCTGGAACTGGGCTGATCACTGGGTTTACTGGGTTGGCCCACTCATTGGTGGGGCTCTTGGTGGGCTCATTTGTGACAACTTCTTCATTCTCAAAACCCATGTTCCTCTTTCAAGAGATGATGAAGCTTTCTAATATCATAAATAATTTCCAAAATAAATGTTATCATTAGTTGTGCTTATGCAATTTGTGCAAATTGCTTGATGTAACTTTGTCATTAAGTGAAGATTTAAATTGGATATTGGACTTTTAAAGACATTATATTAAATTATGCTTTTATAAAATGGTACCACTTTTTATTAGGAATTGAATCTTTCTATGGTACCACTTTTGATAATTTTATATGGTTGAAGGTTGAACAAAGATCCCCAC

mRNA sequence

AACCCAACTAACTAACTAGGATGACTGGATGAGTAGGTCATAAAAGGAGGAATATCCATAGAGGGTGGCATAAAGCAACAAAATAATTTTGTCATACAATACAGTACGTACGCCAATGATAATGATGAATTATCATTTTGATTTTTGACCCATCCATCTAATCATGTAATAACATGGACAAAGGAATCTCCACCTTTCCGGTTGTTTCACCCTTCAATTCATTGCTCTTTCTCATTTTAGTATTTATAAATGCACGTTTTTTAACTATATATATATATATATATATAAAAGTGATTTGCACCTCTGATAATTTGTTAACNGCTCTTTCTCATTTTAGTATTTATAAATGCACGTTTTTTAACTATATATATATATATATATATATATAAAAGTGATTTGCACCTCTGATAATTTGTTAACACTATACTCCATATCATCATGGGTAAAATAGCCCTAGGAACAACCCGAGAGGCAACGGAACCAGAATGCATCAATGCTCTCGTTGTCGAATTCATTTGCACCTTCCTCTTCGTCTTCGCCGGTGTTGGATCTGCCATGGCTGCTGATAAAGCAGAAGCAAGTCCAATAGTAGGATTGTTGTTTGTGGGGATAACACATGCATTTGTGGTAGCTGCGATGATTGCTGCCGGATTTAACATTTCCGGCGGTCACTTGAACCCCGCCGTCACTCTTGGTCTTGCAATTGGTGGCAATATTACAATTCTCAGATCTCTACTTTATTGGATTATACAATGTCTTGCTTCTGCTCTGGCTTGTTACTTGCTCACCTATGTCACCAGTGGACTGGTGACTCCTATACACTCTCTTCCAAAGGGGGTAGACCCTCTACAAGGATTGGTGATGGAGGTGATCTTAACATTTTCTCTCCTCTTCACAATATACACCACCCTTGTTGACCAAAAGAAGGGCCCACTTCAAAGCCAAGGAATATTGCTAACTGGGCTTGTGGTTGGGGCCAACATCTTTGCTGGTGGGTTATTCTCTGGGGCATCAATGAATCCAGCAAGGTCATTTGGGCCTGCTCTTGTAAGCTGGAACTGGGCTGATCACTGGGTTTACTGGGTTGGCCCACTCATTGGTGGGGCTCTTGGTGGGCTCATTTGTGACAACTTCTTCATTCTCAAAACCCATGTTCCTCTTTCAAGAGATGATGAAGCTTTCTAATATCATAAATAATTTCCAAAATAAATGTTATCATTAGTTGTGCTTATGCAATTTGTGCAAATTGCTTGATGTAACTTTGTCATTAAGTGAAGATTTAAATTGGATATTGGACTTTTAAAGACATTATATTAAATTATGCTTTTATAAAATGGTACCACTTTTTATTAGGAATTGAATCTTTCTATGGTACCACTTTTGATAATTTTATATGGTTGAAGGTTGAACAAAGATCCCCAC

Coding sequence (CDS)

ATGGGTAAAATAGCCCTAGGAACAACCCGAGAGGCAACGGAACCAGAATGCATCAATGCTCTCGTTGTCGAATTCATTTGCACCTTCCTCTTCGTCTTCGCCGGTGTTGGATCTGCCATGGCTGCTGATAAAGCAGAAGCAAGTCCAATAGTAGGATTGTTGTTTGTGGGGATAACACATGCATTTGTGGTAGCTGCGATGATTGCTGCCGGATTTAACATTTCCGGCGGTCACTTGAACCCCGCCGTCACTCTTGGTCTTGCAATTGGTGGCAATATTACAATTCTCAGATCTCTACTTTATTGGATTATACAATGTCTTGCTTCTGCTCTGGCTTGTTACTTGCTCACCTATGTCACCAGTGGACTGGTGACTCCTATACACTCTCTTCCAAAGGGGGTAGACCCTCTACAAGGATTGGTGATGGAGGTGATCTTAACATTTTCTCTCCTCTTCACAATATACACCACCCTTGTTGACCAAAAGAAGGGCCCACTTCAAAGCCAAGGAATATTGCTAACTGGGCTTGTGGTTGGGGCCAACATCTTTGCTGGTGGGTTATTCTCTGGGGCATCAATGAATCCAGCAAGGTCATTTGGGCCTGCTCTTGTAAGCTGGAACTGGGCTGATCACTGGGTTTACTGGGTTGGCCCACTCATTGGTGGGGCTCTTGGTGGGCTCATTTGTGACAACTTCTTCATTCTCAAAACCCATGTTCCTCTTTCAAGAGATGATGAAGCTTTCTAA

Protein sequence

MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITHAFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVTSGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGANIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVPLSRDDEAF

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo25629.1	Spo25629.1	mRNA

Homology

[Close]

BLAST of Spo25629.1 vs. NCBI nr
Match: gi|902215423|gb|KNA17477.1| (hypothetical protein SOVF_079550 [Spinacia oleracea])

HSP 1 Score: 487.3 bits (1253), Expect = 1.600e-134
Identity = 246/248 (99.19%), Postives = 246/248 (99.19%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH
Sbjct: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT
Sbjct: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            GLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA
Sbjct: 121 GGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGL CDNFFILKTHVP
Sbjct: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLTCDNFFILKTHVP 240

Query: 241 LSRDDEAF 249
           LSRDDEAF
Sbjct: 241 LSRDDEAF 248

[Close]

BLAST of Spo25629.1 vs. NCBI nr
Match: gi|731317245|ref|XP_010668288.1| (PREDICTED: aquaporin TIP4-1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 434.1 bits (1115), Expect = 1.600e-118
Identity = 213/246 (86.59%), Postives = 229/246 (93.09%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KIALGT+REAT+PECI ALVVEFICTFLFVFAGVGSAMAADKA A+ +VGLLFVG TH
Sbjct: 1   MTKIALGTSREATQPECIKALVVEFICTFLFVFAGVGSAMAADKAGATTLVGLLFVGTTH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           AFVVA MIAAGFNISGGHLNPAVTLGLA+GG+IT++RSLLYWIIQC ASALAC  L YVT
Sbjct: 61  AFVVAVMIAAGFNISGGHLNPAVTLGLAMGGHITLIRSLLYWIIQCFASALACLSLNYVT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            GL TPIH LPKGVDPLQGLVMEVILTFSLLFTIYTT+VD KKGPLQ+QGI LTGLVVGA
Sbjct: 121 GGLTTPIHCLPKGVDPLQGLVMEVILTFSLLFTIYTTIVDPKKGPLQTQGIFLTGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NIFAGGLFSGASMNPARSFGPAL+SWNWADHW+YWVGPLIGG LGGLICDNFF+ K+HVP
Sbjct: 181 NIFAGGLFSGASMNPARSFGPALISWNWADHWIYWVGPLIGGGLGGLICDNFFMFKSHVP 240

Query: 241 LSRDDE 247
           LSRD++
Sbjct: 241 LSRDED 246

[Close]

BLAST of Spo25629.1 vs. NCBI nr
Match: gi|1015601371|gb|AMW03269.1| (aquaporin TIP [Tamarix hispida])

HSP 1 Score: 375.2 bits (962), Expect = 9.000e-101
Identity = 178/248 (71.77%), Postives = 209/248 (84.27%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KIALGT+REAT+P+C+ ALVVEFICTFLFVF GVGSAM+A K    P++ L FVG+ H
Sbjct: 1   MAKIALGTSREATQPDCLKALVVEFICTFLFVFVGVGSAMSAGKLSVDPLLSLFFVGLAH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           A VVA MI+AGF +SGGHLNPAVTLGLA+GG+ITI R +LYW  QC ASA AC LL Y+T
Sbjct: 61  ALVVAVMISAGFTVSGGHLNPAVTLGLALGGHITIFRPILYWFDQCAASAAACVLLKYLT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            GL TP+H+L  G D LQG++ME++LTFSLLFT+YTT+VD +KG L+  G LLTGLVVGA
Sbjct: 121 GGLDTPVHTLANGTDYLQGIIMEIVLTFSLLFTVYTTIVDPRKGSLEGLGPLLTGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NI AGG FSGASMNPARSFGPALVS NW DHWVYWVGPLIGG LGG IC+NFFI+++HVP
Sbjct: 181 NIMAGGAFSGASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLGGFICENFFIVRSHVP 240

Query: 241 LSRDDEAF 249
           +SRDD++F
Sbjct: 241 ISRDDQSF 248

[Close]

BLAST of Spo25629.1 vs. NCBI nr
Match: gi|257480319|gb|ACV60358.1| (putative tonoplast intrinsic protein [Camellia sinensis])

HSP 1 Score: 354.0 bits (907), Expect = 2.200e-94
Identity = 177/248 (71.37%), Postives = 202/248 (81.45%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KIALGT REAT+ +CI+AL+VEFICTFLFVFAGVGSAMAADK     +VGL FV + H
Sbjct: 1   MVKIALGTGREATQIDCIHALIVEFICTFLFVFAGVGSAMAADKLGGDSLVGLFFVAMAH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           A VVA MI+AGF ISGGHLNPAVTLGL +GG+IT+ RS+LYWI Q LASA AC LL Y+T
Sbjct: 61  ALVVAVMISAGFRISGGHLNPAVTLGLCVGGHITVFRSILYWIDQLLASAAACALLKYLT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            GL TP+H+L  G+D  QG++ME+ILTFSLLF++Y   VD KKG L   G LL GLVVGA
Sbjct: 121 GGLATPVHTLASGMDYSQGVIMEIILTFSLLFSVYALCVDPKKGFLDGLGPLLVGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NI AGG FSGASMNPARSFGPALVS NW DHWVYWVGPLIGG L GLI +NFFI+++HVP
Sbjct: 181 NIMAGGPFSGASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGLIYENFFIVRSHVP 240

Query: 241 LSRDDEAF 249
           L R+DE F
Sbjct: 241 LPREDETF 248

[Close]

BLAST of Spo25629.1 vs. NCBI nr
Match: gi|697118100|ref|XP_009612974.1| (PREDICTED: aquaporin TIP4-1 [Nicotiana tomentosiformis])

HSP 1 Score: 351.7 bits (901), Expect = 1.100e-93
Identity = 173/245 (70.61%), Postives = 200/245 (81.63%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KIA+G++REA +P+CI AL+VEFICTFLFVFAGVGSAMAA+K    P+V L FV + H
Sbjct: 1   MAKIAVGSSREAIQPDCIQALIVEFICTFLFVFAGVGSAMAANKLNGDPLVSLFFVAMAH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           A VVA  I+AGF ISGGHLNPAVTLGL +GG+IT+ RS+LYWI Q LAS  AC LL Y+T
Sbjct: 61  ALVVAVTISAGFRISGGHLNPAVTLGLCMGGHITVFRSILYWIDQLLASVAACALLNYLT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
           +GL TP+H+L  GV   QG++MEVILTFSLLFT+YTT+VD KKG L+  G LLTGLVVGA
Sbjct: 121 AGLETPVHTLANGVSYGQGIIMEVILTFSLLFTVYTTIVDPKKGILEGMGPLLTGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NI AGG FSGASMNPARSFGPA VS  W DHWVYWVGPLIGG L G IC+NFFI++THVP
Sbjct: 181 NIMAGGPFSGASMNPARSFGPAFVSGIWTDHWVYWVGPLIGGGLAGFICENFFIVRTHVP 240

Query: 241 LSRDD 246
           L  D+
Sbjct: 241 LPSDE 245

[Close]

BLAST of Spo25629.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RF35_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_079550 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 1.100e-134
Identity = 246/248 (99.19%), Postives = 246/248 (99.19%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH
Sbjct: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT
Sbjct: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            GLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA
Sbjct: 121 GGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGL CDNFFILKTHVP
Sbjct: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLTCDNFFILKTHVP 240

Query: 241 LSRDDEAF 249
           LSRDDEAF
Sbjct: 241 LSRDDEAF 248

[Close]

BLAST of Spo25629.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D1L2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g032220 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.100e-118
Identity = 213/246 (86.59%), Postives = 229/246 (93.09%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KIALGT+REAT+PECI ALVVEFICTFLFVFAGVGSAMAADKA A+ +VGLLFVG TH
Sbjct: 1   MTKIALGTSREATQPECIKALVVEFICTFLFVFAGVGSAMAADKAGATTLVGLLFVGTTH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           AFVVA MIAAGFNISGGHLNPAVTLGLA+GG+IT++RSLLYWIIQC ASALAC  L YVT
Sbjct: 61  AFVVAVMIAAGFNISGGHLNPAVTLGLAMGGHITLIRSLLYWIIQCFASALACLSLNYVT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            GL TPIH LPKGVDPLQGLVMEVILTFSLLFTIYTT+VD KKGPLQ+QGI LTGLVVGA
Sbjct: 121 GGLTTPIHCLPKGVDPLQGLVMEVILTFSLLFTIYTTIVDPKKGPLQTQGIFLTGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NIFAGGLFSGASMNPARSFGPAL+SWNWADHW+YWVGPLIGG LGGLICDNFF+ K+HVP
Sbjct: 181 NIFAGGLFSGASMNPARSFGPALISWNWADHWIYWVGPLIGGGLGGLICDNFFMFKSHVP 240

Query: 241 LSRDDE 247
           LSRD++
Sbjct: 241 LSRDED 246

[Close]

BLAST of Spo25629.1 vs. UniProtKB/TrEMBL
Match: A0A145PR92_9CARY (Aquaporin TIP OS=Tamarix hispida GN=TIP3 PE=2 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 6.300e-101
Identity = 178/248 (71.77%), Postives = 209/248 (84.27%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KIALGT+REAT+P+C+ ALVVEFICTFLFVF GVGSAM+A K    P++ L FVG+ H
Sbjct: 1   MAKIALGTSREATQPDCLKALVVEFICTFLFVFVGVGSAMSAGKLSVDPLLSLFFVGLAH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           A VVA MI+AGF +SGGHLNPAVTLGLA+GG+ITI R +LYW  QC ASA AC LL Y+T
Sbjct: 61  ALVVAVMISAGFTVSGGHLNPAVTLGLALGGHITIFRPILYWFDQCAASAAACVLLKYLT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            GL TP+H+L  G D LQG++ME++LTFSLLFT+YTT+VD +KG L+  G LLTGLVVGA
Sbjct: 121 GGLDTPVHTLANGTDYLQGIIMEIVLTFSLLFTVYTTIVDPRKGSLEGLGPLLTGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NI AGG FSGASMNPARSFGPALVS NW DHWVYWVGPLIGG LGG IC+NFFI+++HVP
Sbjct: 181 NIMAGGAFSGASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLGGFICENFFIVRSHVP 240

Query: 241 LSRDDEAF 249
           +SRDD++F
Sbjct: 241 ISRDDQSF 248

[Close]

BLAST of Spo25629.1 vs. UniProtKB/TrEMBL
Match: D3GDK8_CAMSI (Putative tonoplast intrinsic protein OS=Camellia sinensis PE=2 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.500e-94
Identity = 177/248 (71.37%), Postives = 202/248 (81.45%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KIALGT REAT+ +CI+AL+VEFICTFLFVFAGVGSAMAADK     +VGL FV + H
Sbjct: 1   MVKIALGTGREATQIDCIHALIVEFICTFLFVFAGVGSAMAADKLGGDSLVGLFFVAMAH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           A VVA MI+AGF ISGGHLNPAVTLGL +GG+IT+ RS+LYWI Q LASA AC LL Y+T
Sbjct: 61  ALVVAVMISAGFRISGGHLNPAVTLGLCVGGHITVFRSILYWIDQLLASAAACALLKYLT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            GL TP+H+L  G+D  QG++ME+ILTFSLLF++Y   VD KKG L   G LL GLVVGA
Sbjct: 121 GGLATPVHTLASGMDYSQGVIMEIILTFSLLFSVYALCVDPKKGFLDGLGPLLVGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NI AGG FSGASMNPARSFGPALVS NW DHWVYWVGPLIGG L GLI +NFFI+++HVP
Sbjct: 181 NIMAGGPFSGASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGLIYENFFIVRSHVP 240

Query: 241 LSRDDEAF 249
           L R+DE F
Sbjct: 241 LPREDETF 248

[Close]

BLAST of Spo25629.1 vs. UniProtKB/TrEMBL
Match: Q9XG70_TOBAC (Aquaglyceroporin (Tonoplast intrinsic protein (Tipa)) OS=Nicotiana tabacum GN=TIPa PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 7.400e-94
Identity = 173/245 (70.61%), Postives = 200/245 (81.63%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KIA+G++REA +P+CI AL+VEFICTFLFVFAGVGSAMAA+K    P+V L FV + H
Sbjct: 1   MAKIAVGSSREAIQPDCIQALIVEFICTFLFVFAGVGSAMAANKLNGDPLVSLFFVAMAH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           A VVA  I+AGF ISGGHLNPAVTLGL +GG+IT+ RS+LYWI Q LAS  AC LL Y+T
Sbjct: 61  ALVVAVTISAGFRISGGHLNPAVTLGLCMGGHITVFRSILYWIDQLLASVAACALLNYLT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
           +GL TP+H+L  GV   QG++MEVILTFSLLFT+YTT+VD KKG L+  G LLTGLVVGA
Sbjct: 121 AGLETPVHTLANGVSYGQGIIMEVILTFSLLFTVYTTIVDPKKGILEGMGPLLTGLVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NI AGG FSGASMNPARSFGPA VS  W DHWVYWVGPLIGG L G IC+NFFI++THVP
Sbjct: 181 NIMAGGPFSGASMNPARSFGPAFVSGIWTDHWVYWVGPLIGGGLAGFICENFFIVRTHVP 240

Query: 241 LSRDD 246
           L  D+
Sbjct: 241 LPSDE 245

[Close]

BLAST of Spo25629.1 vs. ExPASy Swiss-Prot
Match: TIP41_ARATH (Aquaporin TIP4-1 OS=Arabidopsis thaliana GN=TIP4-1 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 4.800e-86
Identity = 160/246 (65.04%), Postives = 191/246 (77.64%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KI LG   EA +P+CI AL+VEFI TFLFVFAGVGSAMA D    + +VGL  V + H
Sbjct: 1   MKKIELGHHSEAAKPDCIKALIVEFITTFLFVFAGVGSAMATDSLVGNTLVGLFAVAVAH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           AFVVA MI+AG +ISGGHLNPAVTLGL +GG+I++ R+ LYWI Q LAS+ AC+LL+Y+T
Sbjct: 61  AFVVAVMISAG-HISGGHLNPAVTLGLLLGGHISVFRAFLYWIDQLLASSAACFLLSYLT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            G+ TP+H+L  GV   QG++ E+ILTFSLLFT+Y T+VD KKG L   G LLTG VVGA
Sbjct: 121 GGMGTPVHTLASGVSYTQGIIWEIILTFSLLFTVYATIVDPKKGSLDGFGPLLTGFVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NI AGG FSGASMNPARSFGPALVS NW DHWVYWVGPLIGG L G I +N  I + HVP
Sbjct: 181 NILAGGAFSGASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGFIYENVLIDRPHVP 240

Query: 241 LSRDDE 247
           ++ D++
Sbjct: 241 VADDEQ 245

[Close]

BLAST of Spo25629.1 vs. ExPASy Swiss-Prot
Match: TIP44_MAIZE (Aquaporin TIP4-4 OS=Zea mays GN=TIP4-4 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 6.300e-70
Identity = 145/255 (56.86%), Postives = 177/255 (69.41%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEAS---PIVGLLFVG 60
           M K ALG  REA++  C+ A++ E I TFLFVFAGVGSAMA  K        +VGL  V 
Sbjct: 1   MAKFALGHHREASDAGCVRAVLAELILTFLFVFAGVGSAMATGKLAGGGGDTVVGLTAVA 60

Query: 61  ITHAFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLT 120
           + H  VVA M++AG ++SGGH+NPAVTLGLA  G IT+ RS LY   Q L S LAC LL 
Sbjct: 61  LAHTLVVAVMVSAGLHVSGGHINPAVTLGLAATGRITLFRSALYVAAQLLGSTLACLLLA 120

Query: 121 YVT---SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLT 180
           ++    SG+  P+H+L  GV  L+G++ME +LTFSLLF +Y T+VD ++  +   G LL 
Sbjct: 121 FLAVADSGV--PVHALGAGVGALRGVLMEAVLTFSLLFAVYATVVDPRRA-VGGMGPLLV 180

Query: 181 GLVVGANIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFI 240
           GLVVGAN+ AGG FSGASMNPARSFGPALV+  WADHWVYWVGPLIGG L GL+ D  F+
Sbjct: 181 GLVVGANVLAGGPFSGASMNPARSFGPALVAGVWADHWVYWVGPLIGGPLAGLVYDGLFM 240

Query: 241 LK-THVPLSRDDEAF 249
            +  H PL RDD  F
Sbjct: 241 AQGGHEPLPRDDTDF 252

[Close]

BLAST of Spo25629.1 vs. ExPASy Swiss-Prot
Match: TIP43_ORYSJ (Probable aquaporin TIP4-3 OS=Oryza sativa subsp. japonica GN=TIP4-3 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 1.800e-69
Identity = 142/252 (56.35%), Postives = 177/252 (70.24%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADK--AEASPIVGLLFVGI 60
           M K+ALG  REAT+P C+ A+V E + TFLFVF+GVGSAMAA K       I+GL  V  
Sbjct: 1   MAKLALGHHREATDPGCLRAVVAELLLTFLFVFSGVGSAMAAAKLGGGGDTIMGLTAVAA 60

Query: 61  THAFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTY 120
            HA VVA M++AG ++SGGH+NPAVTLGLA GG+IT+ RS LY   Q L S+LAC LL  
Sbjct: 61  AHALVVAVMVSAGLHVSGGHINPAVTLGLAAGGHITLFRSALYAAAQLLGSSLACLLLAA 120

Query: 121 VTSGL-VTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLV 180
           +T G    P+H+   GV   + + ME +LTFSLLF +Y T+VD+++  + + G LL GLV
Sbjct: 121 LTGGEEAVPVHAPAPGVGAARAVAMEAVLTFSLLFAVYATVVDRRRA-VGALGPLLVGLV 180

Query: 181 VGANIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFI-LK 240
           VGANI AGG +SGASMNPARSFGPAL +  WADHW+YWVGPLIGG L GL+ +  F+   
Sbjct: 181 VGANILAGGPYSGASMNPARSFGPALAAGEWADHWIYWVGPLIGGPLAGLVYEGLFMGPP 240

Query: 241 THVPLSRDDEAF 249
            H PL R+D  F
Sbjct: 241 GHEPLPRNDGDF 251

[Close]

BLAST of Spo25629.1 vs. ExPASy Swiss-Prot
Match: TIP_ANTMA (Probable aquaporin TIP-type OS=Antirrhinum majus GN=DIP PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 7.200e-66
Identity = 130/249 (52.21%), Postives = 168/249 (67.47%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIV---GLLFVG 60
           M KIA G+  ++     I A V EFI T LFVFAGVGSA+A +K  +   +   GL+ V 
Sbjct: 1   MVKIAFGSIGDSFSVASIKAYVAEFIATLLFVFAGVGSAIAYNKLTSDAALDPAGLVAVA 60

Query: 61  ITHAFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLT 120
           + HAF +   ++   N+SGGHLNPAVTLGLA+GGNITIL  L YWI QCL S +AC LL 
Sbjct: 61  VAHAFALFVGVSMAANVSGGHLNPAVTLGLAVGGNITILTGLFYWIAQCLGSTVACLLLK 120

Query: 121 YVTSGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLV 180
           +VT+GL  P H +  G+D +QG+VME+I+TF+L++T+Y T  D KKG L     +  G +
Sbjct: 121 FVTNGLSVPTHGVAAGMDAIQGVVMEIIITFALVYTVYATAADPKKGSLGVIAPIAIGFI 180

Query: 181 VGANIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKT 240
           VGANI A G FSG SMNPARSFGPA+ S +++ +W+YW GPLIGGAL G I  + FI   
Sbjct: 181 VGANILAAGPFSGGSMNPARSFGPAVASGDFSQNWIYWAGPLIGGALAGFIYGDVFI-TA 240

Query: 241 HVPLSRDDE 247
           H PL   ++
Sbjct: 241 HAPLPTSED 248

[Close]

BLAST of Spo25629.1 vs. ExPASy Swiss-Prot
Match: TIP42_MAIZE (Aquaporin TIP4-2 OS=Zea mays GN=TIP4-2 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.600e-65
Identity = 129/242 (53.31%), Postives = 173/242 (71.49%), Query Frame = 1


		  
Query: 11  EATEPE--CINALVVEFICTFLFVFAGVGSAMAADKA----EASPIVGLLFVGITHAFVV 70
           EA  P+  C+ A++ E + TFLFVF GV ++MAA       EA P+  L  V I HA   
Sbjct: 16  EAPAPDVGCVRAVLAELVLTFLFVFTGVSASMAAGAGGKPGEAMPMATLAAVAIAHALAA 75

Query: 71  AAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVTSGLV 130
             ++ AGF++SGGHLNPAVT+G+ + G+IT LR+LLY   Q LAS+LAC LL Y++ G+V
Sbjct: 76  GVLVTAGFHVSGGHLNPAVTVGILVRGHITKLRALLYVAAQLLASSLACILLRYLSGGMV 135

Query: 131 TPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGANIFA 190
           TP+H+L  G+ P+QGLVMEVILTFSLLF  Y  ++D  +  +++ G LLTGL+VGAN  A
Sbjct: 136 TPVHALGAGIRPMQGLVMEVILTFSLLFVTYAMILD-PRSQVRTIGPLLTGLIVGANSLA 195

Query: 191 GGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFIL-KTHVPLSR 246
           GG F+GASMNPARSFGPA+ +  W +HWVYW+GPL+GG+L G + ++ F++ KTH PL  
Sbjct: 196 GGNFTGASMNPARSFGPAMATGVWTNHWVYWIGPLLGGSLAGFVYESLFMVNKTHEPLLN 255

[Close]

BLAST of Spo25629.1 vs. TAIR (Arabidopsis)
Match: AT2G25810.1 (tonoplast intrinsic protein 4;1)

HSP 1 Score: 318.9 bits (816), Expect = 2.700e-87
Identity = 160/246 (65.04%), Postives = 191/246 (77.64%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIVGLLFVGITH 60
           M KI LG   EA +P+CI AL+VEFI TFLFVFAGVGSAMA D    + +VGL  V + H
Sbjct: 1   MKKIELGHHSEAAKPDCIKALIVEFITTFLFVFAGVGSAMATDSLVGNTLVGLFAVAVAH 60

Query: 61  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 120
           AFVVA MI+AG +ISGGHLNPAVTLGL +GG+I++ R+ LYWI Q LAS+ AC+LL+Y+T
Sbjct: 61  AFVVAVMISAG-HISGGHLNPAVTLGLLLGGHISVFRAFLYWIDQLLASSAACFLLSYLT 120

Query: 121 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 180
            G+ TP+H+L  GV   QG++ E+ILTFSLLFT+Y T+VD KKG L   G LLTG VVGA
Sbjct: 121 GGMGTPVHTLASGVSYTQGIIWEIILTFSLLFTVYATIVDPKKGSLDGFGPLLTGFVVGA 180

Query: 181 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 240
           NI AGG FSGASMNPARSFGPALVS NW DHWVYWVGPLIGG L G I +N  I + HVP
Sbjct: 181 NILAGGAFSGASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGFIYENVLIDRPHVP 240

Query: 241 LSRDDE 247
           ++ D++
Sbjct: 241 VADDEQ 245

[Close]

BLAST of Spo25629.1 vs. TAIR (Arabidopsis)
Match: AT2G36830.1 (gamma tonoplast intrinsic protein)

HSP 1 Score: 247.3 bits (630), Expect = 1.000e-65
Identity = 124/245 (50.61%), Postives = 158/245 (64.49%), Query Frame = 1


		  
Query: 4   IALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAE---ASPIVGLLFVGITH 63
           IA+G   EAT P+ + A + EFI T +FV AG GS MA +K     A+   GL+   + H
Sbjct: 6   IAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKLTENGATTPSGLVAAAVAH 65

Query: 64  AFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLTYVT 123
           AF +   ++ G NISGGH+NPAVT G  IGGNIT+LR +LYWI Q L S +AC +L + T
Sbjct: 66  AFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLILKFAT 125

Query: 124 SGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLVVGA 183
            GL  P   L  GV  L   V E+++TF L++T+Y T +D K G L +   +  G +VGA
Sbjct: 126 GGLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDPKNGSLGTIAPIAIGFIVGA 185

Query: 184 NIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFILKTHVP 243
           NI AGG FSGASMNPA +FGPA+VSW W +HWVYW GPL+GG + GLI + FFI  TH  
Sbjct: 186 NILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVYWAGPLVGGGIAGLIYEVFFINTTHEQ 245

Query: 244 LSRDD 246
           L   D
Sbjct: 246 LPTTD 250

[Close]

BLAST of Spo25629.1 vs. TAIR (Arabidopsis)
Match: AT3G16240.1 (delta tonoplast integral protein)

HSP 1 Score: 245.7 bits (626), Expect = 2.900e-65
Identity = 126/249 (50.60%), Postives = 166/249 (66.67%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIV---GLLFVG 60
           M  +A G+  ++     + A + EFI T LFVFAGVGSA+A  K  +   +   GL+ + 
Sbjct: 1   MAGVAFGSFDDSFSLASLRAYLAEFISTLLFVFAGVGSAIAYAKLTSDAALDTPGLVAIA 60

Query: 61  ITHAFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLT 120
           + H F +   +A G NISGGH+NPAVT GLA+GG IT++  + YWI Q L S  AC+LL 
Sbjct: 61  VCHGFALFVAVAIGANISGGHVNPAVTFGLAVGGQITVITGVFYWIAQLLGSTAACFLLK 120

Query: 121 YVTSGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLV 180
           YVT GL  P HS+  G+  ++G+VME+I+TF+L++T+Y T  D KKG L +   L  GL+
Sbjct: 121 YVTGGLAVPTHSVAAGLGSIEGVVMEIIITFALVYTVYATAADPKKGSLGTIAPLAIGLI 180

Query: 181 VGANIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFI-LK 240
           VGANI A G FSG SMNPARSFGPA+ + +++ HWVYWVGPLIGG L GLI  N F+   
Sbjct: 181 VGANILAAGPFSGGSMNPARSFGPAVAAGDFSGHWVYWVGPLIGGGLAGLIYGNVFMGSS 240

Query: 241 THVPLSRDD 246
            HVPL+  D
Sbjct: 241 EHVPLASAD 249

[Close]

BLAST of Spo25629.1 vs. TAIR (Arabidopsis)
Match: AT4G01470.1 (tonoplast intrinsic protein 1;3)

HSP 1 Score: 240.0 bits (611), Expect = 1.600e-63
Identity = 124/250 (49.60%), Postives = 160/250 (64.00%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADK----AEASPIVGLLFV 60
           + +IA+GT  EA+ P+ I A   EF    +FVFAG GS MA  K      A+P  GL+  
Sbjct: 3   INRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGSGMAYGKLTGDGPATP-AGLVAA 62

Query: 61  GITHAFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLL 120
            ++HAF +   ++ G N+SGGH+NPAVT G  IGGNIT+LR++LYWI Q L + +AC LL
Sbjct: 63  SLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIGGNITLLRAILYWIAQLLGAVVACLLL 122

Query: 121 TYVTSGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGL 180
              T G+ T   SL  GV P   +V E+++TF L++T+Y T VD KKG +     L  GL
Sbjct: 123 KVSTGGMETAAFSLSYGVTPWNAVVFEIVMTFGLVYTVYATAVDPKKGDIGIIAPLAIGL 182

Query: 181 VVGANIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFI-L 240
           +VGANI  GG F GASMNPA SFGPA+VSW W +HWVYWVGP IG A+  ++ D  FI  
Sbjct: 183 IVGANILVGGAFDGASMNPAVSFGPAVVSWIWTNHWVYWVGPFIGAAIAAIVYDTIFIGS 242

Query: 241 KTHVPLSRDD 246
             H PL  +D
Sbjct: 243 NGHEPLPSND 251

[Close]

BLAST of Spo25629.1 vs. TAIR (Arabidopsis)
Match: AT4G17340.1 (tonoplast intrinsic protein 2;2)

HSP 1 Score: 236.9 bits (603), Expect = 1.400e-62
Identity = 120/237 (50.63%), Postives = 162/237 (68.35%), Query Frame = 1


		  
Query: 1   MGKIALGTTREATEPECINALVVEFICTFLFVFAGVGSAMAADKAEASPIV---GLLFVG 60
           M KI +G+  ++     + A + EFI T LFVFAGVGSA+A  K  +   +   GL+ V 
Sbjct: 1   MVKIEIGSVGDSFSVASLKAYLSEFIATLLFVFAGVGSALAFAKLTSDAALDPAGLVAVA 60

Query: 61  ITHAFVVAAMIAAGFNISGGHLNPAVTLGLAIGGNITILRSLLYWIIQCLASALACYLLT 120
           + HAF +   ++   NISGGHLNPAVTLGLA+GGNIT++    YWI QCL S +AC LL 
Sbjct: 61  VAHAFALFVGVSIAANISGGHLNPAVTLGLAVGGNITVITGFFYWIAQCLGSIVACLLLV 120

Query: 121 YVTSGLVTPIHSLPKGVDPLQGLVMEVILTFSLLFTIYTTLVDQKKGPLQSQGILLTGLV 180
           +VT+G   P H +  G+  ++G+VME+++TF+L++T+Y T  D KKG L +   +  G +
Sbjct: 121 FVTNGESVPTHGVAAGLGAIEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFI 180

Query: 181 VGANIFAGGLFSGASMNPARSFGPALVSWNWADHWVYWVGPLIGGALGGLICDNFFI 235
           VGANI A G FSG SMNPARSFGPA+VS +++  W+YWVGPL+GGAL GLI  + FI
Sbjct: 181 VGANILAAGPFSGGSMNPARSFGPAVVSGDFSQIWIYWVGPLVGGALAGLIYGDVFI 237

The following BLAST results are available for this feature:

BLAST of Spo25629.1 vs. NCBI nr
BLAST of Spo25629.1 vs. UniProtKB/TrEMBL
BLAST of Spo25629.1 vs. ExPASy Swiss-Prot
BLAST of Spo25629.1 vs. TAIR (Arabidopsis)

BLAST of Spo25629.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902215423\|gb\|KNA17477.1\|	1.6e-134	99.1	hypothetical protein SOVF_0795...	[more]
gi\|731317245\|ref\|XP_010668288.1\|	1.6e-118	86.5	PREDICTED: aquaporin TIP4-1 [B...	[more]
gi\|1015601371\|gb\|AMW03269.1\|	9.0e-101	71.7	aquaporin TIP [Tamarix hispida...	[more]
gi\|257480319\|gb\|ACV60358.1\|	2.2e-94	71.3	putative tonoplast intrinsic p...	[more]
gi\|697118100\|ref\|XP_009612974.1\|	1.1e-93	70.6	PREDICTED: aquaporin TIP4-1 [N...	[more]

BLAST of Spo25629.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RF35_SPIOL	1.1e-134	99.1	Uncharacterized protein OS=Spi...	[more]
A0A0J8D1L2_BETVU	1.1e-118	86.5	Uncharacterized protein OS=Bet...	[more]
A0A145PR92_9CARY	6.3e-101	71.7	Aquaporin TIP OS=Tamarix hispi...	[more]
D3GDK8_CAMSI	1.5e-94	71.3	Putative tonoplast intrinsic p...	[more]
Q9XG70_TOBAC	7.4e-94	70.6	Aquaglyceroporin (Tonoplast in...	[more]

BLAST of Spo25629.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
TIP41_ARATH	4.8e-86	65.0	Aquaporin TIP4-1 OS=Arabidopsi...	[more]
TIP44_MAIZE	6.3e-70	56.8	Aquaporin TIP4-4 OS=Zea mays G...	[more]
TIP43_ORYSJ	1.8e-69	56.3	Probable aquaporin TIP4-3 OS=O...	[more]
TIP_ANTMA	7.2e-66	52.2	Probable aquaporin TIP-type OS...	[more]
TIP42_MAIZE	1.6e-65	53.3	Aquaporin TIP4-2 OS=Zea mays G...	[more]

BLAST of Spo25629.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT2G25810.1	2.7e-87	65.0	tonoplast intrinsic protein 4;...	[more]
AT2G36830.1	1.0e-65	50.6	gamma tonoplast intrinsic prot...	[more]
AT3G16240.1	2.9e-65	50.6	delta tonoplast integral prote...	[more]
AT4G01470.1	1.6e-63	49.6	tonoplast intrinsic protein 1;...	[more]
AT4G17340.1	1.4e-62	50.6	tonoplast intrinsic protein 2;...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 142..160 score: 1.8E-43coord: 212..232 score: 1.8E-43coord: 19..38 score: 1.8E-43coord: 60..84 score: 1.8E-43coord: 97..116 score: 1.8E-43coord: 175..197 score: 1.8
IPR000425	Major intrinsic protein	PANTHER	PTHR19139	AQUAPORIN TRANSPORTER	coord: 10..246 score: 2.9
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 13..228 score: 3.7
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 78..86 scor
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10		coord: 12..239 score: 9.5
IPR023271	Aquaporin-like	unknown	SSF81338	Aquaporin-like	coord: 12..242 score: 2.75
None	No IPR available	PANTHER	PTHR19139:SF143	SUBFAMILY NOT NAMED	coord: 10..246 score: 2.9

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009736	cytokinin-activated signaling pathway
biological_process	GO:0009809	lignin biosynthetic process
biological_process	GO:0009811	stilbene biosynthetic process
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0009611	response to wounding
biological_process	GO:0006810	transport
biological_process	GO:0006598	polyamine catabolic process
biological_process	GO:0032259	methylation
biological_process	GO:0009963	positive regulation of flavonoid biosynthetic process
biological_process	GO:0051555	flavonol biosynthetic process
biological_process	GO:0009805	coumarin biosynthetic process
biological_process	GO:0042398	cellular modified amino acid biosynthetic process
cellular_component	GO:0009506	plasmodesma
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0042807	central vacuole
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009705	plant-type vacuole membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0005215	transporter activity
molecular_function	GO:0047763	caffeate O-methyltransferase activity
molecular_function	GO:0030744	luteolin O-methyltransferase activity
molecular_function	GO:0033799	myricetin 3'-O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0030755	quercetin 3-O-methyltransferase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo16465	0.69	Barchart \| Table
Spo22378	0.66	Barchart \| Table