Spo18410 (gene)

Overview
NameSpo18410
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionCalcium binding
Locationchr1 : 32948890 .. 32949441 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAGGCATTCGCAAGCTCTACCAATTGCTTGTAAGCAATGTTGTAATACCTTCTCTAGGAGTAGTCCCTCACTCAAACGTCCGAAACTCGATTTCCAACCCTACCCTCCCAACCCCTTGCAAGATAAATGCGGTTCAAGCCCACGAGTCGAGGGCAAAAGCGTTAATAACCGTGTTTGGCATGGACAAAGATGGAAGAATAGCTAAAGAGAAAGCGTGCAAGGTGGTTAAACAATTAGGGTTAGTCAAGGAAGAAGAAGAAGAATCGAGTTTCGATATATTAGAGGAAGAAATCGAGGCGAATGAGGTTCTCAACGAGCTCGAGGAAGAAGAGAAGAGAGAAAAACAACAACTTCTTAGACAAGCTTTCACGGTGTTTGATGAGGATGGTGATGGTTTTATTGATGCAAAGGAGATTAAGAGGGTTTTGGTGTGTTTAGGGTTGGATAATGGATGGGATTTGAGTGAAATTGAGAAAATGATTAATGTTGTTGATCTTAATCTTGATGGAAAAGTTGATTTCCATGAATTTGAGTTGATGATGGGTTAA

mRNA sequence

ATGTTAGGCATTCGCAAGCTCTACCAATTGCTTGTAAGCAATGTTGTAATACCTTCTCTAGGAGTAGTCCCTCACTCAAACGTCCGAAACTCGATTTCCAACCCTACCCTCCCAACCCCTTGCAAGATAAATGCGGTTCAAGCCCACGAGTCGAGGGCAAAAGCGTTAATAACCGTGTTTGGCATGGACAAAGATGGAAGAATAGCTAAAGAGAAAGCGTGCAAGGTGGTTAAACAATTAGGGTTAGTCAAGGAAGAAGAAGAAGAATCGAGTTTCGATATATTAGAGGAAGAAATCGAGGCGAATGAGGTTCTCAACGAGCTCGAGGAAGAAGAGAAGAGAGAAAAACAACAACTTCTTAGACAAGCTTTCACGGTGTTTGATGAGGATGGTGATGGTTTTATTGATGCAAAGGAGATTAAGAGGGTTTTGGTGTGTTTAGGGTTGGATAATGGATGGGATTTGAGTGAAATTGAGAAAATGATTAATGTTGTTGATCTTAATCTTGATGGAAAAGTTGATTTCCATGAATTTGAGTTGATGATGGGTTAA

Coding sequence (CDS)

ATGTTAGGCATTCGCAAGCTCTACCAATTGCTTGTAAGCAATGTTGTAATACCTTCTCTAGGAGTAGTCCCTCACTCAAACGTCCGAAACTCGATTTCCAACCCTACCCTCCCAACCCCTTGCAAGATAAATGCGGTTCAAGCCCACGAGTCGAGGGCAAAAGCGTTAATAACCGTGTTTGGCATGGACAAAGATGGAAGAATAGCTAAAGAGAAAGCGTGCAAGGTGGTTAAACAATTAGGGTTAGTCAAGGAAGAAGAAGAAGAATCGAGTTTCGATATATTAGAGGAAGAAATCGAGGCGAATGAGGTTCTCAACGAGCTCGAGGAAGAAGAGAAGAGAGAAAAACAACAACTTCTTAGACAAGCTTTCACGGTGTTTGATGAGGATGGTGATGGTTTTATTGATGCAAAGGAGATTAAGAGGGTTTTGGTGTGTTTAGGGTTGGATAATGGATGGGATTTGAGTGAAATTGAGAAAATGATTAATGTTGTTGATCTTAATCTTGATGGAAAAGTTGATTTCCATGAATTTGAGTTGATGATGGGTTAA

Protein sequence

MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVFGMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFELMMG
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo18410.1Spo18410.1mRNA


Homology
[Close]
BLAST of Spo18410.1 vs. NCBI nr
Match: gi|902233323|gb|KNA22997.1| (hypothetical protein SOVF_028870 [Spinacia oleracea])

HSP 1 Score: 349.4 bits (895), Expect = 3.900e-93
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1 

		  

Query: 1   MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVF 60
           MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVF
Sbjct: 1   MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVF 60

Query: 61  GMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLL 120
           GMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLL
Sbjct: 61  GMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLL 120

Query: 121 RQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFEL 180
           RQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFEL
Sbjct: 121 RQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFEL 180

Query: 181 MMG 184
           MMG
Sbjct: 181 MMG 183
[Close]
BLAST of Spo18410.1 vs. NCBI nr
Match: gi|731317991|ref|XP_010669481.1| (PREDICTED: calmodulin-like protein 2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 306.2 bits (783), Expect = 3.800e-80
Identity = 163/183 (89.07%), Postives = 170/183 (92.90%), Query Frame = 1 

		  

Query: 1   MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVF 60
           MLGIRKLYQLLVSNVV PSLGVVPHSN  NS SN  LP PCK++ VQAHESRAKALITVF
Sbjct: 1   MLGIRKLYQLLVSNVV-PSLGVVPHSNALNSNSNYALPIPCKMDVVQAHESRAKALITVF 60

Query: 61  GMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLL 120
           GMDKDGRIAKEKAC VVKQLGLVKEEEEESSFDILEE++EA EVL ELEEEEKREK+QLL
Sbjct: 61  GMDKDGRIAKEKACNVVKQLGLVKEEEEESSFDILEEQLEAKEVLIELEEEEKREKEQLL 120

Query: 121 RQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFEL 180
           RQAF VFDEDGDGFIDAKEIKRVL+CLGLDNGWD+SEIEKMI VVDLNLDGKVDFHEFEL
Sbjct: 121 RQAFMVFDEDGDGFIDAKEIKRVLMCLGLDNGWDMSEIEKMIKVVDLNLDGKVDFHEFEL 180

Query: 181 MMG 184
           MMG
Sbjct: 181 MMG 182
[Close]
BLAST of Spo18410.1 vs. NCBI nr
Match: gi|470110056|ref|XP_004291303.1| (PREDICTED: calmodulin-like protein 7 [Fragaria vesca subsp. vesca])

HSP 1 Score: 162.2 bits (409), Expect = 8.900e-37
Identity = 97/185 (52.43%), Postives = 126/185 (68.11%), Query Frame = 1 

		  

Query: 1   MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKI--NAVQAHESRAKALIT 60
           M+GI  +   L+ ++V    GV      R S +   +   CK   NA++  ES A+ALIT
Sbjct: 1   MMGITGVCSRLIGDLVHAFHGVS-----RCSSTEVHIDEDCKQPQNAIKVDESMARALIT 60

Query: 61  VFGMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQ 120
           VFGM+ +GRI KEKA +VVK LGL+  +EE+   D    E+   EVL ELE +++ E   
Sbjct: 61  VFGMETNGRIKKEKAKRVVKNLGLICYDEEDEDLD----EVPVEEVLGELESDKRNE--- 120

Query: 121 LLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEF 180
           +L +AF +FD DGDGFI+A E+KRVL CLGLDNGWD+++IEKM+ VVDLNLDGKVDF EF
Sbjct: 121 MLLEAFKIFDVDGDGFIEAVELKRVLECLGLDNGWDMAQIEKMVKVVDLNLDGKVDFSEF 173

Query: 181 ELMMG 184
           ELMMG
Sbjct: 181 ELMMG 173
[Close]
BLAST of Spo18410.1 vs. NCBI nr
Match: gi|596290461|ref|XP_007226186.1| (hypothetical protein PRUPE_ppa015775mg [Prunus persica])

HSP 1 Score: 161.4 bits (407), Expect = 1.500e-36
Identity = 87/143 (60.84%), Postives = 112/143 (78.32%), Query Frame = 1 

		  

Query: 44  NAVQAHESRAKALITVFGMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDIL--EEEIEA 103
           NA +  ES A+ALITVFGM+ +GRI KE A +VV+ LGL+  EE++SSFD+   + E+  
Sbjct: 39  NASKVDESMARALITVFGMETNGRIKKENARRVVENLGLICHEEDKSSFDLPGDDHEVPV 98

Query: 104 NEVLNELEEEEKREKQ-QLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEK 163
            EVL  LEEE+   K+ +LL +AF +FDEDGDGFI+A E+KRVL CLGL +GWD+ +IEK
Sbjct: 99  EEVLGGLEEEDDGSKRNELLLEAFKIFDEDGDGFIEAVELKRVLECLGLGSGWDMDQIEK 158

Query: 164 MINVVDLNLDGKVDFHEFELMMG 184
           M+ VVD+NLDGKVDF+EFELMMG
Sbjct: 159 MVRVVDVNLDGKVDFNEFELMMG 181
[Close]
BLAST of Spo18410.1 vs. NCBI nr
Match: gi|694401319|ref|XP_009375717.1| (PREDICTED: calmodulin-like protein 3 [Pyrus x bretschneideri])

HSP 1 Score: 161.0 bits (406), Expect = 2.000e-36
Identity = 83/141 (58.87%), Postives = 110/141 (78.01%), Query Frame = 1 

		  

Query: 45  AVQAHESRAKALITVFGMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDIL--EEEIEAN 104
           A +  ES A+ALITVFGM+ +GRI KEKA +VV+ LGL++ EE++  FD+   + E+   
Sbjct: 47  ATKVDESMARALITVFGMETNGRIKKEKARRVVENLGLIRNEEDQMGFDLPGDDHEVPVE 106

Query: 105 EVLNELEEEEKREKQQLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMI 164
           E+L  LE+ E  E+ +LLR+AF +FDEDG+GFI+A E+KRVL CLGL +GWD+ ++EKM+
Sbjct: 107 EILGRLEDNES-ERSELLREAFKIFDEDGNGFIEAVELKRVLECLGLGSGWDMDQVEKMV 166

Query: 165 NVVDLNLDGKVDFHEFELMMG 184
            VVDLNLDGKVDF EFELMMG
Sbjct: 167 KVVDLNLDGKVDFGEFELMMG 186
[Close]
BLAST of Spo18410.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RVW8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_028870 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 2.700e-93
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1 

		  

Query: 1   MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVF 60
           MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVF
Sbjct: 1   MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVF 60

Query: 61  GMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLL 120
           GMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLL
Sbjct: 61  GMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLL 120

Query: 121 RQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFEL 180
           RQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFEL
Sbjct: 121 RQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFEL 180

Query: 181 MMG 184
           MMG
Sbjct: 181 MMG 183
[Close]
BLAST of Spo18410.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D0U7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g035110 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 2.600e-80
Identity = 163/183 (89.07%), Postives = 170/183 (92.90%), Query Frame = 1 

		  

Query: 1   MLGIRKLYQLLVSNVVIPSLGVVPHSNVRNSISNPTLPTPCKINAVQAHESRAKALITVF 60
           MLGIRKLYQLLVSNVV PSLGVVPHSN  NS SN  LP PCK++ VQAHESRAKALITVF
Sbjct: 1   MLGIRKLYQLLVSNVV-PSLGVVPHSNALNSNSNYALPIPCKMDVVQAHESRAKALITVF 60

Query: 61  GMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLL 120
           GMDKDGRIAKEKAC VVKQLGLVKEEEEESSFDILEE++EA EVL ELEEEEKREK+QLL
Sbjct: 61  GMDKDGRIAKEKACNVVKQLGLVKEEEEESSFDILEEQLEAKEVLIELEEEEKREKEQLL 120

Query: 121 RQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFEL 180
           RQAF VFDEDGDGFIDAKEIKRVL+CLGLDNGWD+SEIEKMI VVDLNLDGKVDFHEFEL
Sbjct: 121 RQAFMVFDEDGDGFIDAKEIKRVLMCLGLDNGWDMSEIEKMIKVVDLNLDGKVDFHEFEL 180

Query: 181 MMG 184
           MMG
Sbjct: 181 MMG 182
[Close]
BLAST of Spo18410.1 vs. UniProtKB/TrEMBL
Match: M5Y644_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015775mg PE=4 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.100e-36
Identity = 87/143 (60.84%), Postives = 112/143 (78.32%), Query Frame = 1 

		  

Query: 44  NAVQAHESRAKALITVFGMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDIL--EEEIEA 103
           NA +  ES A+ALITVFGM+ +GRI KE A +VV+ LGL+  EE++SSFD+   + E+  
Sbjct: 39  NASKVDESMARALITVFGMETNGRIKKENARRVVENLGLICHEEDKSSFDLPGDDHEVPV 98

Query: 104 NEVLNELEEEEKREKQ-QLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEK 163
            EVL  LEEE+   K+ +LL +AF +FDEDGDGFI+A E+KRVL CLGL +GWD+ +IEK
Sbjct: 99  EEVLGGLEEEDDGSKRNELLLEAFKIFDEDGDGFIEAVELKRVLECLGLGSGWDMDQIEK 158

Query: 164 MINVVDLNLDGKVDFHEFELMMG 184
           M+ VVD+NLDGKVDF+EFELMMG
Sbjct: 159 MVRVVDVNLDGKVDFNEFELMMG 181
[Close]
BLAST of Spo18410.1 vs. UniProtKB/TrEMBL
Match: A0A061GJ66_THECC (Calmodulin like 42, putative OS=Theobroma cacao GN=TCM_036785 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.900e-33
Identity = 83/146 (56.85%), Postives = 107/146 (73.29%), Query Frame = 1 

		  

Query: 50  ESRAKALITVFGMDKDGRIAKEKACKVVKQLGLVKEEEEESSFDI------------LEE 109
           +S  +AL  VFGM+ +GRI KEKA +VV++LGLV+ E + ++FD+            LE+
Sbjct: 75  DSMLRALTAVFGMESNGRITKEKARQVVEKLGLVEGEGDATNFDLPGGGGAAAGGGGLED 134

Query: 110 EIEANEVLNELEEEEKREKQQLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSE 169
           E+   EVL    E+   ++ +LLRQAF +FDEDG+GFIDA E+KRVL CLGLDNGWD+ +
Sbjct: 135 EVAVEEVLGGFMEDGS-KRNELLRQAFRIFDEDGNGFIDALELKRVLQCLGLDNGWDMGQ 194

Query: 170 IEKMINVVDLNLDGKVDFHEFELMMG 184
           IEKM+ VVDLNLDGKVDF EFELMMG
Sbjct: 195 IEKMLMVVDLNLDGKVDFREFELMMG 219
[Close]
BLAST of Spo18410.1 vs. UniProtKB/TrEMBL
Match: I3T705_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.200e-33
Identity = 88/169 (52.07%), Postives = 115/169 (68.05%), Query Frame = 1 

		  

Query: 26  SNVRNSISNPTLPTPCKINAVQAHESRAKALITVFGMDKDGRIAKEKACKVVKQLGLVKE 85
           S+  +SIS+   PT    + ++  E   +AL+TVFGM+ +GRI KE A KVV++LGL+  
Sbjct: 20  SSPPSSISHLDQPTCKAQDMIKLGEKMVRALVTVFGMENNGRIKKENARKVVEKLGLMYG 79

Query: 86  EEEE-----SSFDI------LEEEIEANEVLNELEEEEKREKQQLLRQAFTVFDEDGDGF 145
             EE     S F++       ++E+   EVL ELE+  KR   +LLR+AF +FDEDGDG+
Sbjct: 80  NSEEDKNKVSEFELPCEGGFHDDEVPVEEVLGELEDMSKR--SELLREAFKIFDEDGDGY 139

Query: 146 IDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFELMMG 184
           IDA E+KRVL CLGLD GWD++ IE M+ VVDLN DGKVDF EFELMMG
Sbjct: 140 IDALELKRVLDCLGLDKGWDMNTIESMVKVVDLNFDGKVDFTEFELMMG 186
[Close]
BLAST of Spo18410.1 vs. ExPASy Swiss-Prot
Match: CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.600e-12
Identity = 36/63 (57.14%), Postives = 46/63 (73.02%), Query Frame = 1 

		  

Query: 120 LRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFE 179
           L +AF VFDEDGDGFI A E+++VL  LGL    ++ ++EKMI  VD N DG+VDF EF+
Sbjct: 112 LEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFK 171

Query: 180 LMM 183
            MM
Sbjct: 172 NMM 174
[Close]
BLAST of Spo18410.1 vs. ExPASy Swiss-Prot
Match: CML42_ARATH (Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.400e-12
Identity = 39/77 (50.65%), Postives = 51/77 (66.23%), Query Frame = 1 

		  

Query: 106 NELEEEEKREKQQLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVV 165
           NE +     E +  L +AF VFDE+GDGFI A+E++ VL  LGL  G ++  +EKMI  V
Sbjct: 107 NEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSV 166

Query: 166 DLNLDGKVDFHEFELMM 183
           D N DG+VDF EF+ MM
Sbjct: 167 DRNQDGRVDFFEFKNMM 183
[Close]
BLAST of Spo18410.1 vs. ExPASy Swiss-Prot
Match: CML2_ARATH (Calmodulin-like protein 2 OS=Arabidopsis thaliana GN=CML2 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.800e-12
Identity = 50/136 (36.76%), Postives = 76/136 (55.88%), Query Frame = 1 

		  

Query: 59  VFGMDKDGRIAKEKACKVVKQLGLVKEEEE------------ESSFDILEEEIEANEVLN 118
           +F  + DG+IAK +     K +G++  E E            + + DI E      E++ 
Sbjct: 12  MFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVE 71

Query: 119 ELEEEEKREKQQLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVD 178
           E EEEE       +R+AF VFD++GDGFI  +E++ VL  +GL  G  L + +KMI+ VD
Sbjct: 72  EKEEEED------MREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 131

Query: 179 LNLDGKVDFHEFELMM 183
           ++ DG V+F EF+ MM
Sbjct: 132 VDGDGMVNFKEFKQMM 141
[Close]
BLAST of Spo18410.1 vs. ExPASy Swiss-Prot
Match: CML30_ARATH (Probable calcium-binding protein CML30 OS=Arabidopsis thaliana GN=CML30 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 9.900e-12
Identity = 50/120 (41.67%), Postives = 72/120 (60.00%), Query Frame = 1 

		  

Query: 63  DKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLLRQ 122
           D D  I++E+A  V++ LGL   +++      L+E+  A EV +  EE+E   ++  ++Q
Sbjct: 78  DDDIDISREEAEMVMRSLGLFYNDDQ------LQEQYSAKEVSSLFEEKEASLEE--VKQ 137

Query: 123 AFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFELMM 182
           AF VFDE+ DGFIDA E++RVL  LG   G  L     MI  +D N DGK+DF+EF   M
Sbjct: 138 AFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFM 189
[Close]
BLAST of Spo18410.1 vs. ExPASy Swiss-Prot
Match: CML5_ARATH (Calmodulin-like protein 5 OS=Arabidopsis thaliana GN=CML5 PE=2 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 2.200e-11
Identity = 55/170 (32.35%), Postives = 91/170 (53.53%), Query Frame = 1 

		  

Query: 29  RNSISNPT--LPTPCKINAV--QAHESRAKALITVFGMDKDGRIAKEKACKVVKQLGLVK 88
           +NS  +P+  LP+P   +A   +   S  K +  +F  + DGRI KE+    ++ LG+  
Sbjct: 38  KNSPPSPSTMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYI 97

Query: 89  EEEE------------ESSFDILEEEIEANEVLNELEEEEKREKQQLLRQAFTVFDEDGD 148
            +++            +   DI E E   + +++E   + + E++ + + AF VFD+DGD
Sbjct: 98  PDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDM-KDAFNVFDQDGD 157

Query: 149 GFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFELMM 183
           GFI  +E+K V+  LGL  G  L   +KMI  VD + DG+V++ EF  MM
Sbjct: 158 GFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206
[Close]
BLAST of Spo18410.1 vs. TAIR (Arabidopsis)
Match: AT5G44460.1 (calmodulin like 43)

HSP 1 Score: 73.6 bits (179), Expect = 1.500e-13
Identity = 36/63 (57.14%), Postives = 46/63 (73.02%), Query Frame = 1 

		  

Query: 120 LRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFE 179
           L +AF VFDEDGDGFI A E+++VL  LGL    ++ ++EKMI  VD N DG+VDF EF+
Sbjct: 112 LEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFK 171

Query: 180 LMM 183
            MM
Sbjct: 172 NMM 174
[Close]
BLAST of Spo18410.1 vs. TAIR (Arabidopsis)
Match: AT4G20780.1 (calmodulin like 42)

HSP 1 Score: 73.2 bits (178), Expect = 1.900e-13
Identity = 39/77 (50.65%), Postives = 51/77 (66.23%), Query Frame = 1 

		  

Query: 106 NELEEEEKREKQQLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVV 165
           NE +     E +  L +AF VFDE+GDGFI A+E++ VL  LGL  G ++  +EKMI  V
Sbjct: 107 NEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSV 166

Query: 166 DLNLDGKVDFHEFELMM 183
           D N DG+VDF EF+ MM
Sbjct: 167 DRNQDGRVDFFEFKNMM 183
[Close]
BLAST of Spo18410.1 vs. TAIR (Arabidopsis)
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 72.4 bits (176), Expect = 3.300e-13
Identity = 50/136 (36.76%), Postives = 76/136 (55.88%), Query Frame = 1 

		  

Query: 59  VFGMDKDGRIAKEKACKVVKQLGLVKEEEE------------ESSFDILEEEIEANEVLN 118
           +F  + DG+IAK +     K +G++  E E            + + DI E      E++ 
Sbjct: 12  MFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVE 71

Query: 119 ELEEEEKREKQQLLRQAFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVD 178
           E EEEE       +R+AF VFD++GDGFI  +E++ VL  +GL  G  L + +KMI+ VD
Sbjct: 72  EKEEEED------MREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 131

Query: 179 LNLDGKVDFHEFELMM 183
           ++ DG V+F EF+ MM
Sbjct: 132 VDGDGMVNFKEFKQMM 141
[Close]
BLAST of Spo18410.1 vs. TAIR (Arabidopsis)
Match: AT3G29000.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 71.6 bits (174), Expect = 5.600e-13
Identity = 50/120 (41.67%), Postives = 72/120 (60.00%), Query Frame = 1 

		  

Query: 63  DKDGRIAKEKACKVVKQLGLVKEEEEESSFDILEEEIEANEVLNELEEEEKREKQQLLRQ 122
           D D  I++E+A  V++ LGL   +++      L+E+  A EV +  EE+E   ++  ++Q
Sbjct: 78  DDDIDISREEAEMVMRSLGLFYNDDQ------LQEQYSAKEVSSLFEEKEASLEE--VKQ 137

Query: 123 AFTVFDEDGDGFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFELMM 182
           AF VFDE+ DGFIDA E++RVL  LG   G  L     MI  +D N DGK+DF+EF   M
Sbjct: 138 AFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFM 189
[Close]
BLAST of Spo18410.1 vs. TAIR (Arabidopsis)
Match: AT2G43290.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 70.5 bits (171), Expect = 1.200e-12
Identity = 55/170 (32.35%), Postives = 91/170 (53.53%), Query Frame = 1 

		  

Query: 29  RNSISNPT--LPTPCKINAV--QAHESRAKALITVFGMDKDGRIAKEKACKVVKQLGLVK 88
           +NS  +P+  LP+P   +A   +   S  K +  +F  + DGRI KE+    ++ LG+  
Sbjct: 38  KNSPPSPSTMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYI 97

Query: 89  EEEE------------ESSFDILEEEIEANEVLNELEEEEKREKQQLLRQAFTVFDEDGD 148
            +++            +   DI E E   + +++E   + + E++ + + AF VFD+DGD
Sbjct: 98  PDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDM-KDAFNVFDQDGD 157

Query: 149 GFIDAKEIKRVLVCLGLDNGWDLSEIEKMINVVDLNLDGKVDFHEFELMM 183
           GFI  +E+K V+  LGL  G  L   +KMI  VD + DG+V++ EF  MM
Sbjct: 158 GFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206
The following BLAST results are available for this feature:
BLAST of Spo18410.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902233323|gb|KNA22997.1|3.9e-93100.hypothetical protein SOVF_0288... [more]
gi|731317991|ref|XP_010669481.1|3.8e-8089.0PREDICTED: calmodulin-like pro... [more]
gi|470110056|ref|XP_004291303.1|8.9e-3752.4PREDICTED: calmodulin-like pro... [more]
gi|596290461|ref|XP_007226186.1|1.5e-3660.8hypothetical protein PRUPE_ppa... [more]
gi|694401319|ref|XP_009375717.1|2.0e-3658.8PREDICTED: calmodulin-like pro... [more]
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BLAST of Spo18410.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RVW8_SPIOL2.7e-93100.Uncharacterized protein OS=Spi... [more]
A0A0J8D0U7_BETVU2.6e-8089.0Uncharacterized protein OS=Bet... [more]
M5Y644_PRUPE1.1e-3660.8Uncharacterized protein OS=Pru... [more]
A0A061GJ66_THECC1.9e-3356.8Calmodulin like 42, putative O... [more]
I3T705_LOTJA3.2e-3352.0Uncharacterized protein OS=Lot... [more]
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BLAST of Spo18410.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
CML43_ARATH2.6e-1257.1Probable calcium-binding prote... [more]
CML42_ARATH3.4e-1250.6Calcium-binding protein CML42 ... [more]
CML2_ARATH5.8e-1236.7Calmodulin-like protein 2 OS=A... [more]
CML30_ARATH9.9e-1241.6Probable calcium-binding prote... [more]
CML5_ARATH2.2e-1132.3Calmodulin-like protein 5 OS=A... [more]
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BLAST of Spo18410.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT5G44460.11.5e-1357.1calmodulin like 43[more]
AT4G20780.11.9e-1350.6calmodulin like 42[more]
AT4G12860.13.3e-1336.7EF hand calcium-binding protei... [more]
AT3G29000.15.6e-1341.6Calcium-binding EF-hand family... [more]
AT2G43290.11.2e-1232.3Calcium-binding EF-hand family... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 119..182
score: 1.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 119..147
score: 2.1E-4coord: 52..80
score: 87.0coord: 157..183
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 48..83
score: 6.563coord: 115..150
score: 14.96coord: 153..183
score: 11
IPR011992EF-hand domain pairGENE3D1.10.238.10coord: 115..182
score: 5.5E-18coord: 48..83
score: 1.
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 53..182
score: 1.02
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 128..140
scor
NoneNo IPR availableunknownCoilCoilcoord: 92..122
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 60..182
score: 6.1
NoneNo IPR availablePANTHERPTHR10891:SF574CALCIUM-BINDING PROTEIN CML30-RELATEDcoord: 60..182
score: 6.1

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001101 response to acid chemical
biological_process GO:0006508 proteolysis
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0033554 cellular response to stress
biological_process GO:0007178 transmembrane receptor protein serine/threonine kinase signaling pathway
biological_process GO:0009627 systemic acquired resistance
biological_process GO:0006073 cellular glucan metabolic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0019722 calcium-mediated signaling
biological_process GO:0007267 cell-cell signaling
biological_process GO:0042398 cellular modified amino acid biosynthetic process
biological_process GO:0009698 phenylpropanoid metabolic process
biological_process GO:0006598 polyamine catabolic process
biological_process GO:0048767 root hair elongation
biological_process GO:0009266 response to temperature stimulus
biological_process GO:0010224 response to UV-B
biological_process GO:0042218 1-aminocyclopropane-1-carboxylate biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
biological_process GO:0006952 defense response
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0009835 fruit ripening
biological_process GO:0009737 response to abscisic acid
biological_process GO:0010200 response to chitin
biological_process GO:0009723 response to ethylene
biological_process GO:1901700 response to oxygen-containing compound
biological_process GO:0014070 response to organic cyclic compound
biological_process GO:0009725 response to hormone
biological_process GO:0006810 transport
biological_process GO:0009697 salicylic acid biosynthetic process
biological_process GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process GO:0001666 response to hypoxia
biological_process GO:0080136 priming of cellular response to stress
biological_process GO:0050832 defense response to fungus
biological_process GO:0009595 detection of biotic stimulus
biological_process GO:0010229 inflorescence development
biological_process GO:0009867 jasmonic acid mediated signaling pathway
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0000169 activation of MAPK activity involved in osmosensory signaling pathway
biological_process GO:0009738 abscisic acid-activated signaling pathway
biological_process GO:0009409 response to cold
biological_process GO:0043069 negative regulation of programmed cell death
biological_process GO:0019684 photosynthesis, light reaction
biological_process GO:0048481 plant ovule development
biological_process GO:0006612 protein targeting to membrane
biological_process GO:0010120 camalexin biosynthetic process
biological_process GO:0010310 regulation of hydrogen peroxide metabolic process
biological_process GO:0043900 regulation of multi-organism process
biological_process GO:0010363 regulation of plant-type hypersensitive response
biological_process GO:0035304 regulation of protein dephosphorylation
biological_process GO:0071555 cell wall organization
biological_process GO:2000038 regulation of stomatal complex development
biological_process GO:2000037 regulation of stomatal complex patterning
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0042742 defense response to bacterium
cellular_component GO:0005618 cell wall
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005667 transcription factor complex
cellular_component GO:0000785 chromatin
cellular_component GO:0005634 nucleus
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003682 chromatin binding
molecular_function GO:0005215 transporter activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0042802 identical protein binding
molecular_function GO:0016847 1-aminocyclopropane-1-carboxylate synthase activity
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0004871 obsolete signal transducer activity
molecular_function GO:0005179 hormone activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0004707 MAP kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds
molecular_function GO:0004674 protein serine/threonine kinase activity
RNA-Seq Expression