Spo24940 (gene)

Overview
NameSpo24940
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionCalcium binding protein
Locationchr1 : 30150216 .. 30150668 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAAAACTCAAACTCTCAGAAAAAATCAACAACCGAAATCGAGAGCATATTCCAGAAATTCGACGCAAACGGCGATGGAAAGATCTCAGCGACAGAACTGCAATCTCTGTTACAAGCCCTAGGATCGGAGACGTCGTCGGAAGAGGTGAAGAAGATGATGGACGAGATGGACAAAGATGGCGACGGTTACGTCGATCTAAGGGAACTCGAGGAGTTTCACGCCGGATCTGGCGGCAATGCGGGGTCCACTAAGGAGCTTCGAGAAGCATTCAGCTTGTATGATAAGGATGGCAATGGTAAGATTTCAGCGCGTGAGTTGTACGATGTGTTGAAGAGTCTTGGAGAGAAGTGTTCGTTGAAGGATTGTAAGAAGATGATTCAATCTGTTGATGCGGATGGTGATGGTCATGTTGATTTCGAGGAGTTTAAGAAGATGATGGCCGCTTGA

mRNA sequence

ATGGCGAAAAACTCAAACTCTCAGAAAAAATCAACAACCGAAATCGAGAGCATATTCCAGAAATTCGACGCAAACGGCGATGGAAAGATCTCAGCGACAGAACTGCAATCTCTGTTACAAGCCCTAGGATCGGAGACGTCGTCGGAAGAGGTGAAGAAGATGATGGACGAGATGGACAAAGATGGCGACGGTTACGTCGATCTAAGGGAACTCGAGGAGTTTCACGCCGGATCTGGCGGCAATGCGGGGTCCACTAAGGAGCTTCGAGAAGCATTCAGCTTGTATGATAAGGATGGCAATGGTAAGATTTCAGCGCGTGAGTTGTACGATGTGTTGAAGAGTCTTGGAGAGAAGTGTTCGTTGAAGGATTGTAAGAAGATGATTCAATCTGTTGATGCGGATGGTGATGGTCATGTTGATTTCGAGGAGTTTAAGAAGATGATGGCCGCTTGA

Coding sequence (CDS)

ATGGCGAAAAACTCAAACTCTCAGAAAAAATCAACAACCGAAATCGAGAGCATATTCCAGAAATTCGACGCAAACGGCGATGGAAAGATCTCAGCGACAGAACTGCAATCTCTGTTACAAGCCCTAGGATCGGAGACGTCGTCGGAAGAGGTGAAGAAGATGATGGACGAGATGGACAAAGATGGCGACGGTTACGTCGATCTAAGGGAACTCGAGGAGTTTCACGCCGGATCTGGCGGCAATGCGGGGTCCACTAAGGAGCTTCGAGAAGCATTCAGCTTGTATGATAAGGATGGCAATGGTAAGATTTCAGCGCGTGAGTTGTACGATGTGTTGAAGAGTCTTGGAGAGAAGTGTTCGTTGAAGGATTGTAAGAAGATGATTCAATCTGTTGATGCGGATGGTGATGGTCATGTTGATTTCGAGGAGTTTAAGAAGATGATGGCCGCTTGA

Protein sequence

MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDADGDGHVDFEEFKKMMAA
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo24940.1Spo24940.1mRNA


Homology
[Close]
BLAST of Spo24940.1 vs. NCBI nr
Match: gi|902208551|gb|KNA15979.1| (hypothetical protein SOVF_093330 [Spinacia oleracea])

HSP 1 Score: 287.0 bits (733), Expect = 2.000e-74
Identity = 149/150 (99.33%), Postives = 149/150 (99.33%), Query Frame = 1 

		  

Query: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60
           MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK
Sbjct: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60

Query: 61  DGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120
           DGDGYVDLRE EEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS
Sbjct: 61  DGDGYVDLREFEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120

Query: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           LKDCKKMIQSVDADGDGHVDFEEFKKMMAA
Sbjct: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 150
[Close]
BLAST of Spo24940.1 vs. NCBI nr
Match: gi|731316034|ref|XP_010693960.1| (PREDICTED: probable calcium-binding protein CML18 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 246.1 bits (627), Expect = 3.800e-62
Identity = 130/150 (86.67%), Postives = 134/150 (89.33%), Query Frame = 1 

		  

Query: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60
           MAKNS        EIE IF KFDANGDGKISATEL+SLLQALGSETS+EEVKKMM+EMDK
Sbjct: 1   MAKNS--------EIERIFSKFDANGDGKISATELKSLLQALGSETSTEEVKKMMEEMDK 60

Query: 61  DGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120
           DGDG+VDL E  EFHAGSGGNAGSTKEL EAF LYDKDGNGKISARELYDVLKSLGEKCS
Sbjct: 61  DGDGFVDLLEFSEFHAGSGGNAGSTKELEEAFRLYDKDGNGKISARELYDVLKSLGEKCS 120

Query: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           LKDCKKMI SVDADGDGHVDFEEFKKMMAA
Sbjct: 121 LKDCKKMISSVDADGDGHVDFEEFKKMMAA 142
[Close]
BLAST of Spo24940.1 vs. NCBI nr
Match: gi|356501039|ref|XP_003519336.1| (PREDICTED: probable calcium-binding protein CML18 [Glycine max])

HSP 1 Score: 180.6 bits (457), Expect = 2.000e-42
Identity = 85/135 (62.96%), Postives = 111/135 (82.22%), Query Frame = 1 

		  

Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS TEL+ +L ALGS+T+ EE+K+MM+E+D++GDG++DL+E  +
Sbjct: 4   EVRQIFNKFDKNGDGKISVTELKDMLAALGSKTTDEELKRMMEELDQNGDGFIDLKEFAD 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH   G     +KELR+AF LYD D NG ISA+EL+DVL++LGEKCSL DC++MI +VDA
Sbjct: 64  FHCNGGAGKDDSKELRDAFDLYDVDKNGLISAKELHDVLRNLGEKCSLSDCRRMISNVDA 123

Query: 134 DGDGHVDFEEFKKMM 149
           DGDG+V+FEEFKKMM
Sbjct: 124 DGDGNVNFEEFKKMM 138
[Close]
BLAST of Spo24940.1 vs. NCBI nr
Match: gi|950987490|ref|XP_014503786.1| (PREDICTED: probable calcium-binding protein CML23 [Vigna radiata var. radiata])

HSP 1 Score: 179.1 bits (453), Expect = 5.700e-42
Identity = 88/136 (64.71%), Postives = 110/136 (80.88%), Query Frame = 1 

		  

Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS TEL+ +L  LGS+T+ EE+K+MM+E+DK+GDGY+DL+E  +
Sbjct: 4   EVRQIFNKFDKNGDGKISITELRDMLGTLGSKTTKEELKRMMEELDKNGDGYIDLKEFAD 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH    GN  S KELR+AF LYD D NG I+A+EL+DVL+ LGEKCSL DC++MI +VDA
Sbjct: 64  FHCNDAGNDDS-KELRDAFDLYDVDKNGLITAKELHDVLRKLGEKCSLSDCRRMISNVDA 123

Query: 134 DGDGHVDFEEFKKMMA 150
           DGDG V+FEEFKKMMA
Sbjct: 124 DGDGSVNFEEFKKMMA 138
[Close]
BLAST of Spo24940.1 vs. NCBI nr
Match: gi|593800710|ref|XP_007163392.1| (hypothetical protein PHAVU_001G231000g [Phaseolus vulgaris])

HSP 1 Score: 178.7 bits (452), Expect = 7.500e-42
Identity = 88/135 (65.19%), Postives = 111/135 (82.22%), Query Frame = 1 

		  

Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS +EL+ L+ ALGS+T++EEV++MM E+D++GDGY+DL+E  E
Sbjct: 4   EVRKIFNKFDKNGDGKISCSELRELMAALGSKTTTEEVRRMMAELDRNGDGYIDLKEFGE 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH G GG  G  KELREAF LYD D NG ISA+EL+ V++ LGEKCSL DC++MI +VDA
Sbjct: 64  FHCGGGGGDG--KELREAFELYDLDKNGLISAKELHSVMRRLGEKCSLSDCRRMIGNVDA 123

Query: 134 DGDGHVDFEEFKKMM 149
           DGDG+V+FEEFKKMM
Sbjct: 124 DGDGNVNFEEFKKMM 136
[Close]
BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RAE4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_093330 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 1.400e-74
Identity = 149/150 (99.33%), Postives = 149/150 (99.33%), Query Frame = 1 

		  

Query: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60
           MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK
Sbjct: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60

Query: 61  DGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120
           DGDGYVDLRE EEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS
Sbjct: 61  DGDGYVDLREFEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120

Query: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           LKDCKKMIQSVDADGDGHVDFEEFKKMMAA
Sbjct: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 150
[Close]
BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CYG9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g027860 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 2.700e-62
Identity = 130/150 (86.67%), Postives = 134/150 (89.33%), Query Frame = 1 

		  

Query: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60
           MAKNS        EIE IF KFDANGDGKISATEL+SLLQALGSETS+EEVKKMM+EMDK
Sbjct: 1   MAKNS--------EIERIFSKFDANGDGKISATELKSLLQALGSETSTEEVKKMMEEMDK 60

Query: 61  DGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120
           DGDG+VDL E  EFHAGSGGNAGSTKEL EAF LYDKDGNGKISARELYDVLKSLGEKCS
Sbjct: 61  DGDGFVDLLEFSEFHAGSGGNAGSTKELEEAFRLYDKDGNGKISARELYDVLKSLGEKCS 120

Query: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           LKDCKKMI SVDADGDGHVDFEEFKKMMAA
Sbjct: 121 LKDCKKMISSVDADGDGHVDFEEFKKMMAA 142
[Close]
BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: I1JHX0_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G245700 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.400e-42
Identity = 85/135 (62.96%), Postives = 111/135 (82.22%), Query Frame = 1 

		  

Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS TEL+ +L ALGS+T+ EE+K+MM+E+D++GDG++DL+E  +
Sbjct: 4   EVRQIFNKFDKNGDGKISVTELKDMLAALGSKTTDEELKRMMEELDQNGDGFIDLKEFAD 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH   G     +KELR+AF LYD D NG ISA+EL+DVL++LGEKCSL DC++MI +VDA
Sbjct: 64  FHCNGGAGKDDSKELRDAFDLYDVDKNGLISAKELHDVLRNLGEKCSLSDCRRMISNVDA 123

Query: 134 DGDGHVDFEEFKKMM 149
           DGDG+V+FEEFKKMM
Sbjct: 124 DGDGNVNFEEFKKMM 138
[Close]
BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: V7D2D5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G231000g PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 5.200e-42
Identity = 88/135 (65.19%), Postives = 111/135 (82.22%), Query Frame = 1 

		  

Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS +EL+ L+ ALGS+T++EEV++MM E+D++GDGY+DL+E  E
Sbjct: 4   EVRKIFNKFDKNGDGKISCSELRELMAALGSKTTTEEVRRMMAELDRNGDGYIDLKEFGE 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH G GG  G  KELREAF LYD D NG ISA+EL+ V++ LGEKCSL DC++MI +VDA
Sbjct: 64  FHCGGGGGDG--KELREAFELYDLDKNGLISAKELHSVMRRLGEKCSLSDCRRMIGNVDA 123

Query: 134 DGDGHVDFEEFKKMM 149
           DGDG+V+FEEFKKMM
Sbjct: 124 DGDGNVNFEEFKKMM 136
[Close]
BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: A0A0L9UWX6_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan07g073800 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.500e-41
Identity = 88/136 (64.71%), Postives = 109/136 (80.15%), Query Frame = 1 

		  

Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS TEL+ +L  LGS+T+ EE+K+MM+E+DK+GDGY+DL+E  +
Sbjct: 4   EVRQIFNKFDKNGDGKISITELRDMLGTLGSKTTKEELKRMMEELDKNGDGYIDLKEFAD 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH    GN  S KELR+AF LYD D NG I+A+EL+DVL  LGEKCSL DC++MI +VDA
Sbjct: 64  FHCNDAGNDDS-KELRDAFDLYDVDKNGLITAKELHDVLCKLGEKCSLSDCRRMISNVDA 123

Query: 134 DGDGHVDFEEFKKMMA 150
           DGDG V+FEEFKKMMA
Sbjct: 124 DGDGSVNFEEFKKMMA 138
[Close]
BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 9.200e-40
Identity = 87/147 (59.18%), Postives = 107/147 (72.79%), Query Frame = 1 

		  

Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+E +F+++DANGDGKISA EL S+L+ALG+     EV++MMDEMD D DG+VDL E   
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 74  FHAGS----GGNAGSTK--------ELREAFSLYDKDGNGKISARELYDVLKSLGEKCSL 133
           FH G     GG  G  K        ELREAF +YD D NGKISAREL+ VL+ LG+KCS+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 134 KDCKKMIQSVDADGDGHVDFEEFKKMM 149
            DC +MI+SVDADGDG V+F+EFKKMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151
[Close]
BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 1.800e-35
Identity = 76/143 (53.15%), Postives = 100/143 (69.93%), Query Frame = 1 

		  

Query: 11  STTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRE 70
           S  +I+ +FQ+FD NGDGKIS  EL+ +++AL    S EE   MM + D DG+G++DL E
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDE 73

Query: 71  LEEFH----AGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKK 130
                     G G N     +L+EAF LYD DGNG+ISA+EL+ V+K+LGEKCS++DCKK
Sbjct: 74  FVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKK 133

Query: 131 MIQSVDADGDGHVDFEEFKKMMA 150
           MI  VD DGDG V+F+EFKKMM+
Sbjct: 134 MISKVDIDGDGCVNFDEFKKMMS 156
[Close]
BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 9.800e-34
Identity = 78/151 (51.66%), Postives = 104/151 (68.87%), Query Frame = 1 

		  

Query: 1   MAKN-SNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMD 60
           M+KN S +   S  +I+ +FQ+FD N DGKIS  EL+ ++ AL    S EE K MM E D
Sbjct: 1   MSKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFD 60

Query: 61  KDGDGYVDLRELEEFH--AGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGE 120
            DG+G++DL E       +    N  + ++L+EAF LYD D NG+ISA EL+ V+K+LGE
Sbjct: 61  LDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGE 120

Query: 121 KCSLKDCKKMIQSVDADGDGHVDFEEFKKMM 149
           KCS++DC++MI  VD+DGDG VDFEEFKKMM
Sbjct: 121 KCSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151
[Close]
BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 1.700e-33
Identity = 71/146 (48.63%), Postives = 103/146 (70.55%), Query Frame = 1 

		  

Query: 3   KNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDG 62
           + + S K    E+E++F+KFD NGDGKIS+ EL +++ +LG E   EE++K + E+D+ G
Sbjct: 26  QKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKG 85

Query: 63  DGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLK 122
           DGY++  E  E +          + L++AFS+YD DGNG ISA EL++VL+SLG++CS+ 
Sbjct: 86  DGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIA 145

Query: 123 DCKKMIQSVDADGDGHVDFEEFKKMM 149
           +C+KMI  VD DGDG +DFEEFK MM
Sbjct: 146 ECRKMIGGVDKDGDGTIDFEEFKIMM 171
[Close]
BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.000e-32
Identity = 75/157 (47.77%), Postives = 107/157 (68.15%), Query Frame = 1 

		  

Query: 1   MAKNSNSQKKSTT------EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKM 60
           MA N++   K +       E++ +F +FDANGDGKIS  EL  +L+ALGS TS EE+ ++
Sbjct: 1   MAANTDRNSKPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRI 60

Query: 61  MDEMDKDGDGYVDLRELEEFHAGSGG---NAGSTKELREAFSLYDKDGNGKISARELYDV 120
           M+E+D D DG+++++E   F         ++G   EL+EAF LYD+D NG IS+ EL+ +
Sbjct: 61  MEEIDTDKDGFINVQEFAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKI 120

Query: 121 LKSLGEKCSLKDCKKMIQSVDADGDGHVDFEEFKKMM 149
           L  LGE+ +  DC +MI+SVD+DGDG+V FEEFKKMM
Sbjct: 121 LTRLGERYAEHDCVEMIKSVDSDGDGYVSFEEFKKMM 157
[Close]
BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT5G37770.1 (EF hand calcium-binding protein family)

HSP 1 Score: 150.2 bits (378), Expect = 1.000e-36
Identity = 76/143 (53.15%), Postives = 100/143 (69.93%), Query Frame = 1 

		  

Query: 11  STTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRE 70
           S  +I+ +FQ+FD NGDGKIS  EL+ +++AL    S EE   MM + D DG+G++DL E
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDE 73

Query: 71  LEEFH----AGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKK 130
                     G G N     +L+EAF LYD DGNG+ISA+EL+ V+K+LGEKCS++DCKK
Sbjct: 74  FVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKK 133

Query: 131 MIQSVDADGDGHVDFEEFKKMMA 150
           MI  VD DGDG V+F+EFKKMM+
Sbjct: 134 MISKVDIDGDGCVNFDEFKKMMS 156
[Close]
BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT1G66400.1 (calmodulin like 23)

HSP 1 Score: 144.4 bits (363), Expect = 5.500e-35
Identity = 78/151 (51.66%), Postives = 104/151 (68.87%), Query Frame = 1 

		  

Query: 1   MAKN-SNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMD 60
           M+KN S +   S  +I+ +FQ+FD N DGKIS  EL+ ++ AL    S EE K MM E D
Sbjct: 1   MSKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFD 60

Query: 61  KDGDGYVDLRELEEFH--AGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGE 120
            DG+G++DL E       +    N  + ++L+EAF LYD D NG+ISA EL+ V+K+LGE
Sbjct: 61  LDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGE 120

Query: 121 KCSLKDCKKMIQSVDADGDGHVDFEEFKKMM 149
           KCS++DC++MI  VD+DGDG VDFEEFKKMM
Sbjct: 121 KCSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151
[Close]
BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 143.7 bits (361), Expect = 9.400e-35
Identity = 71/146 (48.63%), Postives = 103/146 (70.55%), Query Frame = 1 

		  

Query: 3   KNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDG 62
           + + S K    E+E++F+KFD NGDGKIS+ EL +++ +LG E   EE++K + E+D+ G
Sbjct: 26  QKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKG 85

Query: 63  DGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLK 122
           DGY++  E  E +          + L++AFS+YD DGNG ISA EL++VL+SLG++CS+ 
Sbjct: 86  DGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIA 145

Query: 123 DCKKMIQSVDADGDGHVDFEEFKKMM 149
           +C+KMI  VD DGDG +DFEEFK MM
Sbjct: 146 ECRKMIGGVDKDGDGTIDFEEFKIMM 171
[Close]
BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 131.3 bits (329), Expect = 4.800e-31
Identity = 66/137 (48.18%), Postives = 95/137 (69.34%), Query Frame = 1 

		  

Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E++ +F +FD+NGDGKIS  EL  + +A+G+  +  E+ ++++E+D D DGY++L E   
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
               S     S  E+R+AF LYD+D NG ISA EL+ VL  LG  CS++DC +MI  VDA
Sbjct: 83  LCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 134 DGDGHVDFEEFKKMMAA 151
           DGDG+V+FEEF+KMM +
Sbjct: 143 DGDGNVNFEEFQKMMTS 155
[Close]
BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 129.4 bits (324), Expect = 1.800e-30
Identity = 70/152 (46.05%), Postives = 103/152 (67.76%), Query Frame = 1 

		  

Query: 4   NSNSQKKSTT-----EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEM 63
           N  S  KSTT     E++ +F KFDANGDGKIS +EL ++ +++G+  + EE+ +++DE+
Sbjct: 5   NLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEI 64

Query: 64  DKDGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEK 123
           D D DG+++  E       S     S  E+REAF LYD++ NG IS+ E++ VL  LG  
Sbjct: 65  DIDCDGFINQEEFATICRSSS----SAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMT 124

Query: 124 CSLKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           CS++DC +MI  VD DGDG+V+FEEF+KMM++
Sbjct: 125 CSVEDCVRMIGHVDTDGDGNVNFEEFQKMMSS 152
The following BLAST results are available for this feature:
BLAST of Spo24940.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902208551|gb|KNA15979.1|2.0e-7499.3hypothetical protein SOVF_0933... [more]
gi|731316034|ref|XP_010693960.1|3.8e-6286.6PREDICTED: probable calcium-bi... [more]
gi|356501039|ref|XP_003519336.1|2.0e-4262.9PREDICTED: probable calcium-bi... [more]
gi|950987490|ref|XP_014503786.1|5.7e-4264.7PREDICTED: probable calcium-bi... [more]
gi|593800710|ref|XP_007163392.1|7.5e-4265.1hypothetical protein PHAVU_001... [more]
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BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RAE4_SPIOL1.4e-7499.3Uncharacterized protein OS=Spi... [more]
A0A0J8CYG9_BETVU2.7e-6286.6Uncharacterized protein OS=Bet... [more]
I1JHX0_SOYBN1.4e-4262.9Uncharacterized protein OS=Gly... [more]
V7D2D5_PHAVU5.2e-4265.1Uncharacterized protein OS=Pha... [more]
A0A0L9UWX6_PHAAN1.5e-4164.7Uncharacterized protein OS=Pha... [more]
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BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
CML18_ORYSJ9.2e-4059.1Probable calcium-binding prote... [more]
CML24_ARATH1.8e-3553.1Calcium-binding protein CML24 ... [more]
CML23_ARATH9.8e-3451.6Probable calcium-binding prote... [more]
CML25_ARATH1.7e-3348.6Probable calcium-binding prote... [more]
ALL8_OLEEU7.0e-3247.7Calcium-binding allergen Ole e... [more]
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BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT5G37770.11.0e-3653.1EF hand calcium-binding protei... [more]
AT1G66400.15.5e-3551.6calmodulin like 23[more]
AT1G24620.19.4e-3548.6EF hand calcium-binding protei... [more]
AT1G18210.14.8e-3148.1Calcium-binding EF-hand family... [more]
AT1G73630.11.8e-3046.0EF hand calcium-binding protei... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 86..148
score: 8.3E-13coord: 14..74
score: 5.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 87..115
score: 3.5E-7coord: 50..78
score: 6.7E-4coord: 123..150
score: 0.0021coord: 14..42
score: 4.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 119..150
score: 12.7coord: 83..118
score: 15.741coord: 10..45
score: 15.406coord: 46..81
score: 14
IPR011992EF-hand domain pairGENE3D1.10.238.10coord: 6..148
score: 4.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 9..148
score: 4.43
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 96..108
score: -coord: 23..35
score: -coord: 132..144
score: -coord: 59..71
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 14..148
score: 8.6
NoneNo IPR availablePANTHERPTHR10891:SF605CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 14..148
score: 8.6

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process GO:0030001 metal ion transport
biological_process GO:0006446 regulation of translational initiation
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0050832 defense response to fungus
biological_process GO:0009595 detection of biotic stimulus
biological_process GO:0006468 protein phosphorylation
biological_process GO:0006984 ER-nucleus signaling pathway
biological_process GO:0055085 transmembrane transport
biological_process GO:0006888 ER to Golgi vesicle-mediated transport
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0048767 root hair elongation
biological_process GO:0010817 regulation of hormone levels
biological_process GO:0000271 polysaccharide biosynthetic process
biological_process GO:0045454 cell redox homeostasis
biological_process GO:0009825 multidimensional cell growth
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0071555 cell wall organization
biological_process GO:0042742 defense response to bacterium
biological_process GO:0015802 basic amino acid transport
biological_process GO:0043481 anthocyanin accumulation in tissues in response to UV light
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0043069 negative regulation of programmed cell death
biological_process GO:0006366 transcription by RNA polymerase II
biological_process GO:0000394 RNA splicing, via endonucleolytic cleavage and ligation
biological_process GO:0001101 response to acid chemical
biological_process GO:0009987 cellular process
biological_process GO:0044260 cellular macromolecule metabolic process
biological_process GO:0044262 cellular carbohydrate metabolic process
biological_process GO:0009725 response to hormone
biological_process GO:0048193 Golgi vesicle transport
biological_process GO:1901700 response to oxygen-containing compound
biological_process GO:0006073 cellular glucan metabolic process
biological_process GO:0030968 endoplasmic reticulum unfolded protein response
biological_process GO:0010091 trichome branching
biological_process GO:0043090 amino acid import
biological_process GO:0015696 ammonium transport
biological_process GO:0044237 cellular metabolic process
biological_process GO:0065007 biological regulation
biological_process GO:0009932 cell tip growth
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0000165 MAPK cascade
biological_process GO:0042538 hyperosmotic salinity response
biological_process GO:0009620 response to fungus
biological_process GO:0010200 response to chitin
biological_process GO:0010363 regulation of plant-type hypersensitive response
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0006612 protein targeting to membrane
biological_process GO:0009867 jasmonic acid mediated signaling pathway
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0052542 defense response by callose deposition
biological_process GO:0034976 response to endoplasmic reticulum stress
biological_process GO:0043269 regulation of ion transport
biological_process GO:0009697 salicylic acid biosynthetic process
biological_process GO:0009627 systemic acquired resistance
biological_process GO:0002237 response to molecule of bacterial origin
biological_process GO:0031347 regulation of defense response
biological_process GO:0043900 regulation of multi-organism process
biological_process GO:0010337 regulation of salicylic acid metabolic process
biological_process GO:0002679 respiratory burst involved in defense response
biological_process GO:0009414 response to water deprivation
biological_process GO:0016567 protein ubiquitination
biological_process GO:0009611 response to wounding
biological_process GO:0009651 response to salt stress
biological_process GO:0009755 hormone-mediated signaling pathway
biological_process GO:0048519 negative regulation of biological process
biological_process GO:0016310 phosphorylation
biological_process GO:0098542 defense response to other organism
biological_process GO:0009863 salicylic acid mediated signaling pathway
biological_process GO:0043067 regulation of programmed cell death
biological_process GO:0033554 cellular response to stress
biological_process GO:0035556 intracellular signal transduction
biological_process GO:1901701 cellular response to oxygen-containing compound
biological_process GO:0071229 cellular response to acid chemical
biological_process GO:0006464 cellular protein modification process
biological_process GO:0006862 nucleotide transport
biological_process GO:0008152 metabolic process
biological_process GO:0010310 regulation of hydrogen peroxide metabolic process
biological_process GO:0045087 innate immune response
cellular_component GO:0008287 protein serine/threonine phosphatase complex
cellular_component GO:0005736 DNA-directed RNA polymerase I complex
cellular_component GO:0005665 DNA-directed RNA polymerase II, core complex
cellular_component GO:0000419 DNA-directed RNA polymerase V complex
cellular_component GO:0000418 DNA-directed RNA polymerase IV complex
cellular_component GO:0005666 DNA-directed RNA polymerase III complex
cellular_component GO:0016020 membrane
cellular_component GO:0005618 cell wall
cellular_component GO:0048046 apoplast
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005840 ribosome
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009507 chloroplast
molecular_function GO:0015035 protein disulfide oxidoreductase activity
molecular_function GO:0009055 electron transfer activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0051087 chaperone binding
molecular_function GO:0016757 transferase activity, transferring glycosyl groups
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0004721 phosphoprotein phosphatase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0016740 transferase activity
molecular_function GO:0050662 coenzyme binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0001054 RNA polymerase I activity
molecular_function GO:0001055 RNA polymerase II activity
molecular_function GO:0001056 RNA polymerase III activity
molecular_function GO:0047918 GDP-mannose 3,5-epimerase activity
RNA-Seq Expression