Homology
BLAST of Spo25427.1 vs. NCBI nr
Match:
gi|902235999|gb|KNA24171.1| (hypothetical protein SOVF_016590 [Spinacia oleracea])
HSP 1 Score: 1135.2 bits (2935), Expect = 0.000e+0
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 1
Query: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID
Sbjct: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
Query: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST
Sbjct: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
Query: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK
Sbjct: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
Query: 181 YFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN 240
YFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN
Sbjct: 181 YFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN 240
Query: 241 PNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIA 300
PNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIA
Sbjct: 241 PNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIA 300
Query: 301 PFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA 360
PFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA
Sbjct: 301 PFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA 360
Query: 361 GPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKG 420
GPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKG
Sbjct: 361 GPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKG 420
Query: 421 YLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN 480
YLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN
Sbjct: 421 YLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN 480
Query: 481 HPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDS 540
HPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDS
Sbjct: 481 HPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDS 540
Query: 541 IPKLPSGKILRKELRARLANGITTK 566
IPKLPSGKILRKELRARLANGITTK
Sbjct: 541 IPKLPSGKILRKELRARLANGITTK 565
BLAST of Spo25427.1 vs. NCBI nr
Match:
gi|731354276|ref|XP_010688496.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Beta vulgaris subsp. vulgaris])
HSP 1 Score: 997.7 bits (2578), Expect = 8.400e-288
Identity = 494/565 (87.43%), Postives = 525/565 (92.92%), Query Frame = 1
Query: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
MGSE+ H+D +EV +D KE+IF SKLPDIYIPNHLPLHTYCFENL EHG CLID
Sbjct: 1 MGSEEAIPHVDDHEV---LDQKEYIFHSKLPDIYIPNHLPLHTYCFENLHEHGKRPCLID 60
Query: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
GPTGR+WTYEEVE+T+R+VGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIG VST
Sbjct: 61 GPTGREWTYEEVEQTSRRVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGVVST 120
Query: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
TANPFCT EIEKQAR SGA+AILTQSAFADKV+PYC+DNNIHLIVVD +S D E K
Sbjct: 121 TANPFCTPTEIEKQARASGARAILTQSAFADKVKPYCQDNNIHLIVVDNMSFDGSHDEYK 180
Query: 181 YFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN 240
YFWD+VG GEE+ +PE I+P+DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN
Sbjct: 181 YFWDLVGGPGEEEKVPEVKIVPNDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN 240
Query: 241 PNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIA 300
PNLYFNG+DV+LCVLPLFHIYSLNSVLLCALRVGAAIL+MPKF+I MLM+LVQKYKVTIA
Sbjct: 241 PNLYFNGEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMPKFEIYMLMDLVQKYKVTIA 300
Query: 301 PFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA 360
PFVPPIVLAIAKN DMD+YDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA
Sbjct: 301 PFVPPIVLAIAKNPDMDKYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA 360
Query: 361 GPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKG 420
GPVLAMCLAFAKE MEVKSGSCGTVVRNA+LKIVDPDTG SLPRN+ GEICIRG QIMKG
Sbjct: 361 GPVLAMCLAFAKESMEVKSGSCGTVVRNAQLKIVDPDTGFSLPRNKPGEICIRGDQIMKG 420
Query: 421 YLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN 480
YLND EAT NTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN
Sbjct: 421 YLNDQEATANTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN 480
Query: 481 HPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDS 540
HPSIHDAAVVAMQDE AGEVPVAFV R+NGSQI+EDDVKQ+ISKQVVFYKRIY+VFFV+S
Sbjct: 481 HPSIHDAAVVAMQDETAGEVPVAFVVRSNGSQINEDDVKQFISKQVVFYKRIYRVFFVES 540
Query: 541 IPKLPSGKILRKELRARLANGITTK 566
IPKLPSGKILRKELRARLANGI TK
Sbjct: 541 IPKLPSGKILRKELRARLANGIITK 562
BLAST of Spo25427.1 vs. NCBI nr
Match:
gi|1005742903|gb|AMQ23610.1| (4-coumarate:CoA ligase 2 [Silene littorea])
HSP 1 Score: 922.2 bits (2382), Expect = 4.500e-265
Identity = 469/568 (82.57%), Postives = 513/568 (90.32%), Query Frame = 1
Query: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
MGS Q +N EV TKE+IFRSKLPDIYIPNHLPLHTYCFEN+ E G TCLID
Sbjct: 1 MGSIQDNNL----EVLATNPTKEYIFRSKLPDIYIPNHLPLHTYCFENISEFGDRTCLID 60
Query: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
GPTGR+WTY EVEKT+++VG+GF + LG+QKG VIMLLLQN PEFVFSFLGASF+G V T
Sbjct: 61 GPTGREWTYLEVEKTSKRVGSGFCNGLGVQKGSVIMLLLQNCPEFVFSFLGASFVGGVIT 120
Query: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
TANPFCTS EIEKQAR SGA+A++TQSAFADKVR YC +N+I L++VDT S + GP ++K
Sbjct: 121 TANPFCTSGEIEKQARASGARAVITQSAFADKVRTYCDENDIQLVLVDTPSTN-GP-QDK 180
Query: 181 YFWDIVGTQGEEDN----LPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQV 240
YFWD++ ED LPE +I DDVVALPYSSGTTGLPKGVMLTHKSLVTSV QQV
Sbjct: 181 YFWDLM-----EDEVVGVLPEVDINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSVGQQV 240
Query: 241 DGENPNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYK 300
DGENPNLYF+ +DVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKF+I+MLMELVQKYK
Sbjct: 241 DGENPNLYFHNEDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFEIHMLMELVQKYK 300
Query: 301 VTIAPFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYG 360
VTIAPFVPPIVLAIAK+ D+D+YDLSSVR +LSGAAPMGK+LED+VRAKLPNAKLGQGYG
Sbjct: 301 VTIAPFVPPIVLAIAKSPDVDKYDLSSVRTILSGAAPMGKELEDSVRAKLPNAKLGQGYG 360
Query: 361 MTEAGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQ 420
MTEAGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPD+G SLPRNQ GEICIRG+Q
Sbjct: 361 MTEAGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDSGLSLPRNQPGEICIRGAQ 420
Query: 421 IMKGYLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEG 480
IMKGYLNDPEAT+NTIDK+GWLHTGDIGFVD+DDEIFIVDRLKELIKYKGFQVAPAELEG
Sbjct: 421 IMKGYLNDPEATSNTIDKDGWLHTGDIGFVDNDDEIFIVDRLKELIKYKGFQVAPAELEG 480
Query: 481 MLINHPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVF 540
MLINHPSIH+AAVVAM+DEAAGEVPVAFV R NG QI+ED+VKQY+SKQVVFYKRI KVF
Sbjct: 481 MLINHPSIHEAAVVAMKDEAAGEVPVAFVVRLNGCQITEDEVKQYVSKQVVFYKRINKVF 540
Query: 541 FVDSIPKLPSGKILRKELRARLANGITT 565
FVDSIPKLPSGKILRKELRARLANGITT
Sbjct: 541 FVDSIPKLPSGKILRKELRARLANGITT 557
BLAST of Spo25427.1 vs. NCBI nr
Match:
gi|1005742892|gb|AMQ23609.1| (4-coumarate:CoA ligase 1 [Silene littorea])
HSP 1 Score: 893.3 bits (2307), Expect = 2.200e-256
Identity = 444/564 (78.72%), Postives = 492/564 (87.23%), Query Frame = 1
Query: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
M +Q I +E K++IFRSKLPDIYIPNHLPLHTYCFE L E C+ID
Sbjct: 1 MELKQEHYQIHDQVIETTKPLKQYIFRSKLPDIYIPNHLPLHTYCFEKLSEFSDRKCMID 60
Query: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
G TGR+W+Y EVEKTA++VG GF ++ G+QKG VIMLLLQN PEFVFSFLGASFIG +ST
Sbjct: 61 GTTGREWSYMEVEKTAKRVGEGFRNQFGVQKGSVIMLLLQNCPEFVFSFLGASFIGCIST 120
Query: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
TANP CT EIEKQAR S A+ ++TQS FADKVR YC++N+IHL++VD P +K
Sbjct: 121 TANPLCTLGEIEKQARASEARVVITQSTFADKVRTYCEENDIHLVLVD--HPTINGVHDK 180
Query: 181 YFWDIVGTQGEEDNL-PEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGE 240
YFWD+V + + + + I PDDVVALPYSSGTTGLPKGVMLTHKSLVTSV QQVDGE
Sbjct: 181 YFWDLVENESSDPPIIDDIEISPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVGQQVDGE 240
Query: 241 NPNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTI 300
NPNLYF +DV+LCVLPLFHIYSLNSVLLCALRVGAAIL+MPKF+INMLMELVQKYKVTI
Sbjct: 241 NPNLYFQPEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMPKFEINMLMELVQKYKVTI 300
Query: 301 APFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTE 360
APFVPPIVLAIAKNQDM +YDLSSVR ++SGAAPMGKDLEDAVRAKLPNAKLGQGYGMTE
Sbjct: 301 APFVPPIVLAIAKNQDMGKYDLSSVRTIMSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTE 360
Query: 361 AGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMK 420
AGPVLAMCLAFAKEPM+VKSGSCGTVVRNA+LKI+DP+TG SLPRN GEICIRG QIMK
Sbjct: 361 AGPVLAMCLAFAKEPMDVKSGSCGTVVRNADLKIIDPETGSSLPRNHPGEICIRGDQIMK 420
Query: 421 GYLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLI 480
GYLNDPEAT NTIDK+GWLHTGDIG+VDDDDEIFIVDRLKELIKYKGFQVAPAELE ML+
Sbjct: 421 GYLNDPEATMNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLL 480
Query: 481 NHPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVD 540
NHPSI+DAAVVAM+D+AAGEVPVAFV R+NG +I+EDDVKQYISKQVVFYKRI +VFFVD
Sbjct: 481 NHPSIYDAAVVAMKDDAAGEVPVAFVVRSNGCKITEDDVKQYISKQVVFYKRINRVFFVD 540
Query: 541 SIPKLPSGKILRKELRARLANGIT 564
SIPKLPSGKILRKELRARLANGIT
Sbjct: 541 SIPKLPSGKILRKELRARLANGIT 562
BLAST of Spo25427.1 vs. NCBI nr
Match:
gi|9651915|gb|AAF91309.1|AF239686_1 (4-coumarate:coA ligase 2 [Rubus idaeus])
HSP 1 Score: 813.1 bits (2099), Expect = 3.000e-232
Identity = 404/543 (74.40%), Postives = 462/543 (85.08%), Query Frame = 1
Query: 20 DTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKTARQV 79
D EFIFRSKLPDIYIPNHLPLHTYCFEN+ + CLI+G TG +TY EVE T+R+V
Sbjct: 7 DDHEFIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGETFTYAEVELTSRRV 66
Query: 80 GAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQARVSG 139
AG KLG+Q+ V+MLLLQN PEF F+FLGAS+IGA+STTANPF T E+ KQA+ S
Sbjct: 67 AAGLD-KLGIQQNDVVMLLLQNCPEFAFAFLGASYIGAMSTTANPFYTPAEVAKQAKASN 126
Query: 140 AQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGEEDNLPEAN 199
A+ I+TQSA+ DKV+ + K N++ ++ VD S +D H ++ +E P
Sbjct: 127 AKLIITQSAYVDKVKDFAKLNDVKVMCVDETSSEDVLHFSELM------SADESETPAVK 186
Query: 200 IIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVVLCVLPLFH 259
I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYF+ +DV+LCVLPLFH
Sbjct: 187 INPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFH 246
Query: 260 IYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAKNQDMDRY 319
IYSLNSV LC LRVGAAIL+M KF+IN L+ELV+K KVTIAPFVPPIVL+IAK D+ RY
Sbjct: 247 IYSLNSVFLCGLRVGAAILIMQKFEINKLLELVEKEKVTIAPFVPPIVLSIAKCPDLHRY 306
Query: 320 DLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPMEVKS 379
DLSS+R+V+SGAAPMGK+LED VRAKLPNAKLGQGYGMTEAGPVL+MCLAFAKEP E+KS
Sbjct: 307 DLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPYEIKS 366
Query: 380 GSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNTIDKEGWLH 439
G+CGTVVRNAE+KI+DPDT SLPRNQ GEICIRGSQIMKGYLNDPEAT NTIDKEGWLH
Sbjct: 367 GACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPEATENTIDKEGWLH 426
Query: 440 TGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVAMQDEAAGE 499
TGDIG++DDDDE+FIVDRLKELIKYKGFQVAPAELE MLI+HP++ DAAVV+M+DEAAGE
Sbjct: 427 TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVSMKDEAAGE 486
Query: 500 VPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILRKELRARLA 559
VPVAFV R+NGS+ISEDD+KQYISKQVVFYKRI KVFF D IPK PSGKILRK+LRARLA
Sbjct: 487 VPVAFVVRSNGSKISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARLA 542
Query: 560 NGI 563
G+
Sbjct: 547 AGL 542
BLAST of Spo25427.1 vs. UniProtKB/TrEMBL
Match:
A0A0K9RXE1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_016590 PE=4 SV=1)
HSP 1 Score: 1135.2 bits (2935), Expect = 0.000e+0
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 1
Query: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID
Sbjct: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
Query: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST
Sbjct: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
Query: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK
Sbjct: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
Query: 181 YFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN 240
YFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN
Sbjct: 181 YFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN 240
Query: 241 PNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIA 300
PNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIA
Sbjct: 241 PNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIA 300
Query: 301 PFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA 360
PFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA
Sbjct: 301 PFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA 360
Query: 361 GPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKG 420
GPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKG
Sbjct: 361 GPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKG 420
Query: 421 YLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN 480
YLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN
Sbjct: 421 YLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN 480
Query: 481 HPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDS 540
HPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDS
Sbjct: 481 HPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDS 540
Query: 541 IPKLPSGKILRKELRARLANGITTK 566
IPKLPSGKILRKELRARLANGITTK
Sbjct: 541 IPKLPSGKILRKELRARLANGITTK 565
BLAST of Spo25427.1 vs. UniProtKB/TrEMBL
Match:
A0A0J8BPZ1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g198680 PE=4 SV=1)
HSP 1 Score: 997.7 bits (2578), Expect = 5.900e-288
Identity = 494/565 (87.43%), Postives = 525/565 (92.92%), Query Frame = 1
Query: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
MGSE+ H+D +EV +D KE+IF SKLPDIYIPNHLPLHTYCFENL EHG CLID
Sbjct: 1 MGSEEAIPHVDDHEV---LDQKEYIFHSKLPDIYIPNHLPLHTYCFENLHEHGKRPCLID 60
Query: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
GPTGR+WTYEEVE+T+R+VGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIG VST
Sbjct: 61 GPTGREWTYEEVEQTSRRVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGVVST 120
Query: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
TANPFCT EIEKQAR SGA+AILTQSAFADKV+PYC+DNNIHLIVVD +S D E K
Sbjct: 121 TANPFCTPTEIEKQARASGARAILTQSAFADKVKPYCQDNNIHLIVVDNMSFDGSHDEYK 180
Query: 181 YFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN 240
YFWD+VG GEE+ +PE I+P+DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN
Sbjct: 181 YFWDLVGGPGEEEKVPEVKIVPNDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGEN 240
Query: 241 PNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIA 300
PNLYFNG+DV+LCVLPLFHIYSLNSVLLCALRVGAAIL+MPKF+I MLM+LVQKYKVTIA
Sbjct: 241 PNLYFNGEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMPKFEIYMLMDLVQKYKVTIA 300
Query: 301 PFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA 360
PFVPPIVLAIAKN DMD+YDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA
Sbjct: 301 PFVPPIVLAIAKNPDMDKYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEA 360
Query: 361 GPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKG 420
GPVLAMCLAFAKE MEVKSGSCGTVVRNA+LKIVDPDTG SLPRN+ GEICIRG QIMKG
Sbjct: 361 GPVLAMCLAFAKESMEVKSGSCGTVVRNAQLKIVDPDTGFSLPRNKPGEICIRGDQIMKG 420
Query: 421 YLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN 480
YLND EAT NTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN
Sbjct: 421 YLNDQEATANTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLIN 480
Query: 481 HPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDS 540
HPSIHDAAVVAMQDE AGEVPVAFV R+NGSQI+EDDVKQ+ISKQVVFYKRIY+VFFV+S
Sbjct: 481 HPSIHDAAVVAMQDETAGEVPVAFVVRSNGSQINEDDVKQFISKQVVFYKRIYRVFFVES 540
Query: 541 IPKLPSGKILRKELRARLANGITTK 566
IPKLPSGKILRKELRARLANGI TK
Sbjct: 541 IPKLPSGKILRKELRARLANGIITK 562
BLAST of Spo25427.1 vs. UniProtKB/TrEMBL
Match:
A0A142D8E1_9CARY (4-coumarate:CoA ligase 2 OS=Silene littorea PE=2 SV=1)
HSP 1 Score: 922.2 bits (2382), Expect = 3.100e-265
Identity = 469/568 (82.57%), Postives = 513/568 (90.32%), Query Frame = 1
Query: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
MGS Q +N EV TKE+IFRSKLPDIYIPNHLPLHTYCFEN+ E G TCLID
Sbjct: 1 MGSIQDNNL----EVLATNPTKEYIFRSKLPDIYIPNHLPLHTYCFENISEFGDRTCLID 60
Query: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
GPTGR+WTY EVEKT+++VG+GF + LG+QKG VIMLLLQN PEFVFSFLGASF+G V T
Sbjct: 61 GPTGREWTYLEVEKTSKRVGSGFCNGLGVQKGSVIMLLLQNCPEFVFSFLGASFVGGVIT 120
Query: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
TANPFCTS EIEKQAR SGA+A++TQSAFADKVR YC +N+I L++VDT S + GP ++K
Sbjct: 121 TANPFCTSGEIEKQARASGARAVITQSAFADKVRTYCDENDIQLVLVDTPSTN-GP-QDK 180
Query: 181 YFWDIVGTQGEEDN----LPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQV 240
YFWD++ ED LPE +I DDVVALPYSSGTTGLPKGVMLTHKSLVTSV QQV
Sbjct: 181 YFWDLM-----EDEVVGVLPEVDINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSVGQQV 240
Query: 241 DGENPNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYK 300
DGENPNLYF+ +DVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKF+I+MLMELVQKYK
Sbjct: 241 DGENPNLYFHNEDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFEIHMLMELVQKYK 300
Query: 301 VTIAPFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYG 360
VTIAPFVPPIVLAIAK+ D+D+YDLSSVR +LSGAAPMGK+LED+VRAKLPNAKLGQGYG
Sbjct: 301 VTIAPFVPPIVLAIAKSPDVDKYDLSSVRTILSGAAPMGKELEDSVRAKLPNAKLGQGYG 360
Query: 361 MTEAGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQ 420
MTEAGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPD+G SLPRNQ GEICIRG+Q
Sbjct: 361 MTEAGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDSGLSLPRNQPGEICIRGAQ 420
Query: 421 IMKGYLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEG 480
IMKGYLNDPEAT+NTIDK+GWLHTGDIGFVD+DDEIFIVDRLKELIKYKGFQVAPAELEG
Sbjct: 421 IMKGYLNDPEATSNTIDKDGWLHTGDIGFVDNDDEIFIVDRLKELIKYKGFQVAPAELEG 480
Query: 481 MLINHPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVF 540
MLINHPSIH+AAVVAM+DEAAGEVPVAFV R NG QI+ED+VKQY+SKQVVFYKRI KVF
Sbjct: 481 MLINHPSIHEAAVVAMKDEAAGEVPVAFVVRLNGCQITEDEVKQYVSKQVVFYKRINKVF 540
Query: 541 FVDSIPKLPSGKILRKELRARLANGITT 565
FVDSIPKLPSGKILRKELRARLANGITT
Sbjct: 541 FVDSIPKLPSGKILRKELRARLANGITT 557
BLAST of Spo25427.1 vs. UniProtKB/TrEMBL
Match:
A0A142D8E0_9CARY (4-coumarate:CoA ligase 1 OS=Silene littorea PE=2 SV=1)
HSP 1 Score: 893.3 bits (2307), Expect = 1.600e-256
Identity = 444/564 (78.72%), Postives = 492/564 (87.23%), Query Frame = 1
Query: 1 MGSEQVSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLID 60
M +Q I +E K++IFRSKLPDIYIPNHLPLHTYCFE L E C+ID
Sbjct: 1 MELKQEHYQIHDQVIETTKPLKQYIFRSKLPDIYIPNHLPLHTYCFEKLSEFSDRKCMID 60
Query: 61 GPTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVST 120
G TGR+W+Y EVEKTA++VG GF ++ G+QKG VIMLLLQN PEFVFSFLGASFIG +ST
Sbjct: 61 GTTGREWSYMEVEKTAKRVGEGFRNQFGVQKGSVIMLLLQNCPEFVFSFLGASFIGCIST 120
Query: 121 TANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENK 180
TANP CT EIEKQAR S A+ ++TQS FADKVR YC++N+IHL++VD P +K
Sbjct: 121 TANPLCTLGEIEKQARASEARVVITQSTFADKVRTYCEENDIHLVLVD--HPTINGVHDK 180
Query: 181 YFWDIVGTQGEEDNL-PEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGE 240
YFWD+V + + + + I PDDVVALPYSSGTTGLPKGVMLTHKSLVTSV QQVDGE
Sbjct: 181 YFWDLVENESSDPPIIDDIEISPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVGQQVDGE 240
Query: 241 NPNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTI 300
NPNLYF +DV+LCVLPLFHIYSLNSVLLCALRVGAAIL+MPKF+INMLMELVQKYKVTI
Sbjct: 241 NPNLYFQPEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMPKFEINMLMELVQKYKVTI 300
Query: 301 APFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTE 360
APFVPPIVLAIAKNQDM +YDLSSVR ++SGAAPMGKDLEDAVRAKLPNAKLGQGYGMTE
Sbjct: 301 APFVPPIVLAIAKNQDMGKYDLSSVRTIMSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTE 360
Query: 361 AGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMK 420
AGPVLAMCLAFAKEPM+VKSGSCGTVVRNA+LKI+DP+TG SLPRN GEICIRG QIMK
Sbjct: 361 AGPVLAMCLAFAKEPMDVKSGSCGTVVRNADLKIIDPETGSSLPRNHPGEICIRGDQIMK 420
Query: 421 GYLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLI 480
GYLNDPEAT NTIDK+GWLHTGDIG+VDDDDEIFIVDRLKELIKYKGFQVAPAELE ML+
Sbjct: 421 GYLNDPEATMNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLL 480
Query: 481 NHPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVD 540
NHPSI+DAAVVAM+D+AAGEVPVAFV R+NG +I+EDDVKQYISKQVVFYKRI +VFFVD
Sbjct: 481 NHPSIYDAAVVAMKDDAAGEVPVAFVVRSNGCKITEDDVKQYISKQVVFYKRINRVFFVD 540
Query: 541 SIPKLPSGKILRKELRARLANGIT 564
SIPKLPSGKILRKELRARLANGIT
Sbjct: 541 SIPKLPSGKILRKELRARLANGIT 562
BLAST of Spo25427.1 vs. UniProtKB/TrEMBL
Match:
Q9LL50_RUBID (4-coumarate:coA ligase 2 OS=Rubus idaeus PE=2 SV=1)
HSP 1 Score: 813.1 bits (2099), Expect = 2.100e-232
Identity = 404/543 (74.40%), Postives = 462/543 (85.08%), Query Frame = 1
Query: 20 DTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKTARQV 79
D EFIFRSKLPDIYIPNHLPLHTYCFEN+ + CLI+G TG +TY EVE T+R+V
Sbjct: 7 DDHEFIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGETFTYAEVELTSRRV 66
Query: 80 GAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQARVSG 139
AG KLG+Q+ V+MLLLQN PEF F+FLGAS+IGA+STTANPF T E+ KQA+ S
Sbjct: 67 AAGLD-KLGIQQNDVVMLLLQNCPEFAFAFLGASYIGAMSTTANPFYTPAEVAKQAKASN 126
Query: 140 AQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGEEDNLPEAN 199
A+ I+TQSA+ DKV+ + K N++ ++ VD S +D H ++ +E P
Sbjct: 127 AKLIITQSAYVDKVKDFAKLNDVKVMCVDETSSEDVLHFSELM------SADESETPAVK 186
Query: 200 IIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVVLCVLPLFH 259
I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYF+ +DV+LCVLPLFH
Sbjct: 187 INPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFH 246
Query: 260 IYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAKNQDMDRY 319
IYSLNSV LC LRVGAAIL+M KF+IN L+ELV+K KVTIAPFVPPIVL+IAK D+ RY
Sbjct: 247 IYSLNSVFLCGLRVGAAILIMQKFEINKLLELVEKEKVTIAPFVPPIVLSIAKCPDLHRY 306
Query: 320 DLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPMEVKS 379
DLSS+R+V+SGAAPMGK+LED VRAKLPNAKLGQGYGMTEAGPVL+MCLAFAKEP E+KS
Sbjct: 307 DLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPYEIKS 366
Query: 380 GSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNTIDKEGWLH 439
G+CGTVVRNAE+KI+DPDT SLPRNQ GEICIRGSQIMKGYLNDPEAT NTIDKEGWLH
Sbjct: 367 GACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPEATENTIDKEGWLH 426
Query: 440 TGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVAMQDEAAGE 499
TGDIG++DDDDE+FIVDRLKELIKYKGFQVAPAELE MLI+HP++ DAAVV+M+DEAAGE
Sbjct: 427 TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVSMKDEAAGE 486
Query: 500 VPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILRKELRARLA 559
VPVAFV R+NGS+ISEDD+KQYISKQVVFYKRI KVFF D IPK PSGKILRK+LRARLA
Sbjct: 487 VPVAFVVRSNGSKISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARLA 542
Query: 560 NGI 563
G+
Sbjct: 547 AGL 542
BLAST of Spo25427.1 vs. ExPASy Swiss-Prot
Match:
4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1)
HSP 1 Score: 783.1 bits (2021), Expect = 2.000e-225
Identity = 388/541 (71.72%), Postives = 453/541 (83.73%), Query Frame = 1
Query: 23 EFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKTARQVGAG 82
+ IFRSKLPDIYIP HLPLH+YCFENL E S CLIDG R +TY EVE T+R+V G
Sbjct: 12 DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVG 71
Query: 83 FHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQARVSGAQA 142
+ KLG+Q+ IM+LL N PEFVF+F+GAS++GA+ST ANP T E+ KQA+ S A+
Sbjct: 72 LN-KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKI 131
Query: 143 ILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGEEDNLPEANIIP 202
++TQ+ FA KV+ Y +N++ +I VD++ P +F +++ Q +E +P+ I P
Sbjct: 132 VITQACFAGKVKDYAIENDLKVICVDSV-----PEGCVHFSELI--QSDEHEIPDVKIQP 191
Query: 203 DDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVVLCVLPLFHIYS 262
DDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY + DDV++CVLPLFHIYS
Sbjct: 192 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYS 251
Query: 263 LNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAKNQDMDRYDLS 322
LNSVLLCALRVGAAIL+M KFDI +EL+ K+KVTI PFVPPIVLAIAK+ +D YDLS
Sbjct: 252 LNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLS 311
Query: 323 SVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPMEVKSGSC 382
SVR V+SGAAP+GK+LEDAVRAK PNAKLGQGYGMTEAGPVLAMCLAFAKEP ++KSG+C
Sbjct: 312 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 371
Query: 383 GTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNTIDKEGWLHTGD 442
GTVVRNAE+KIVDPDTGCSLPRNQ GEICIRG QIMKGYLNDPEAT TI+KEGWLHTGD
Sbjct: 372 GTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGD 431
Query: 443 IGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVAMQDEAAGEVPV 502
IGF+DDDDE+FIVDRLKELIKYKGFQVAPAELE +LINHP I DAAVV M DE AGEVPV
Sbjct: 432 IGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPV 491
Query: 503 AFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILRKELRARLANGI 562
AFV R+NGS I+ED+VK +ISKQV+FYKRI +VFFV+++PK PSGKILRK+LRARLA GI
Sbjct: 492 AFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGI 544
Query: 563 T 564
+
Sbjct: 552 S 544
BLAST of Spo25427.1 vs. ExPASy Swiss-Prot
Match:
4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)
HSP 1 Score: 781.9 bits (2018), Expect = 4.600e-225
Identity = 385/551 (69.87%), Postives = 457/551 (82.94%), Query Frame = 1
Query: 15 VEGVVDTKE---FIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEE 74
+E +TK+ IFRSKLPDIYIP HLPLH+YCFEN+ E S CLI+G + +TY E
Sbjct: 3 METTTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAE 62
Query: 75 VEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEI 134
VE T R+V G + KLG+Q+ IM+LL NSPEFVF+F+GAS++GA+ST ANP T E+
Sbjct: 63 VELTCRKVAVGLN-KLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEV 122
Query: 135 EKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGE 194
KQA+ S A+ I+TQS F KV+ Y +N++ +I +D+ P +F ++ TQ +
Sbjct: 123 VKQAKASSAKIIITQSCFVGKVKDYASENDVKVICIDS-----APEGCLHFSEL--TQSD 182
Query: 195 EDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVV 254
E +PE I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY + +DV+
Sbjct: 183 EHEIPEVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVL 242
Query: 255 LCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIA 314
+CVLPLFHIYSLNS+LLC LRVGAAIL+M KFDI +EL+QKYKV+I PFVPPIVLAIA
Sbjct: 243 MCVLPLFHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIA 302
Query: 315 KNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFA 374
K+ +D YDLSSVR V+SGAAP+GK+LEDAVR K PNAKLGQGYGMTEAGPVLAMCLAFA
Sbjct: 303 KSPIVDSYDLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFA 362
Query: 375 KEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNT 434
KEP ++KSG+CGTVVRNAE+KIVDPDTGCSLPRNQ GEICIRG QIMKGYLNDPEATT T
Sbjct: 363 KEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRT 422
Query: 435 IDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVA 494
IDKEGWLHTGDIGF+D+DDE+FIVDRLKELIKYKGFQVAPAE+E +L+NHP+I DAAVV
Sbjct: 423 IDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVP 482
Query: 495 MQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILR 554
M+DE AGEVPVAFV R+NGS I+ED+VK +ISKQV+FYKR+ +VFFV+++PK PSGKILR
Sbjct: 483 MKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILR 542
Query: 555 KELRARLANGI 563
K+LRARLA G+
Sbjct: 543 KDLRARLAAGV 545
BLAST of Spo25427.1 vs. ExPASy Swiss-Prot
Match:
4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)
HSP 1 Score: 780.4 bits (2014), Expect = 1.300e-224
Identity = 389/548 (70.99%), Postives = 455/548 (83.03%), Query Frame = 1
Query: 19 VDTKE---FIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKT 78
++TK+ IFRSKLPDIYIP HLPLH+YCFENL E S CLIDG R +TY EVE T
Sbjct: 5 IETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELT 64
Query: 79 ARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQA 138
+R+V G + KLG+Q+ IM+LL N PEFVF+F+GAS++GA+ST ANP T E+ KQA
Sbjct: 65 SRKVAVGLN-KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQA 124
Query: 139 RVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGEEDNL 198
+ S A+ ++TQ+ FA KV+ Y +N++ +I VD+ P +F +++ Q +E +
Sbjct: 125 KASSAKIVITQACFAGKVKDYAIENDLKVICVDS-----APEGCVHFSELI--QSDEHEI 184
Query: 199 PEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVVLCVL 258
P+ I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY + DDV++CVL
Sbjct: 185 PDVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVL 244
Query: 259 PLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAKNQD 318
PLFHIYSLNSVLLCALRVGAAIL+M KFDI +EL+ K+KVTI PFVPPIVLAIAK+
Sbjct: 245 PLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPL 304
Query: 319 MDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPM 378
+ YDLSSVR V+SGAAP+GK+LEDAVRAK PNAKLGQGYGMTEAGPVLAMCLAFAKEP
Sbjct: 305 VHNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 364
Query: 379 EVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNTIDKE 438
++KSG+CGTVVRNAE+KIVDPDTGCSLPRNQ GEICIRG QIMKGYLNDPEAT TI+KE
Sbjct: 365 DIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKE 424
Query: 439 GWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVAMQDE 498
GWLHTGDIGF+DDDDE+FIVDRLKELIKYKGFQVAPAELE +LINHP I DAAVV M DE
Sbjct: 425 GWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDE 484
Query: 499 AAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILRKELR 558
AGEVPVAFV R+NGS I+ED+VK +ISKQV+FYKRI +VFFV+++PK PSGKILRK+LR
Sbjct: 485 QAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLR 544
Query: 559 ARLANGIT 564
ARLA GI+
Sbjct: 545 ARLAAGIS 544
BLAST of Spo25427.1 vs. ExPASy Swiss-Prot
Match:
4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)
HSP 1 Score: 775.8 bits (2002), Expect = 3.300e-223
Identity = 380/538 (70.63%), Postives = 450/538 (83.64%), Query Frame = 1
Query: 22 KEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKTARQVGA 81
K+ IFRSKLPDIYIP +LPLH+YCFEN+ + S CLI+G T +TY +VE +R+VG+
Sbjct: 12 KDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRVGS 71
Query: 82 GFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQARVSGAQ 141
G KLG+++G IM+LL NSPEFVF+FLGASFIG++ST ANPF TS E+ KQA+ S A+
Sbjct: 72 GLS-KLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNAK 131
Query: 142 AILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGEEDNLPEANII 201
I+TQ + DKV+ Y +N + +I +DT + D +F ++ G +E+ +P+ I
Sbjct: 132 LIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGA--DENEMPKVEIS 191
Query: 202 PDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVVLCVLPLFHIY 261
PD VVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY + DDV+LCVLPLFHIY
Sbjct: 192 PDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIY 251
Query: 262 SLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAKNQDMDRYDL 321
SLNSVLLC LR G+ IL+M KF+I +EL+QKYKVTI PFVPPIVLAIAK+ +D YDL
Sbjct: 252 SLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDL 311
Query: 322 SSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPMEVKSGS 381
SSVR V+SGAAP+GK+LEDAVRAK PNAKLGQGYGMTEAGPVLAMCLAFAKEP ++KSG+
Sbjct: 312 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 371
Query: 382 CGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNTIDKEGWLHTG 441
CGTVVRNAE+KIVDP+TG SLPRN GEICIRG QIMKGYLNDPEAT TIDKEGWLHTG
Sbjct: 372 CGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTG 431
Query: 442 DIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVAMQDEAAGEVP 501
DIG++DDDDE+FIVDRLKELIKYKGFQVAPAELE +L+ HP I DAAVV M+DEAAGEVP
Sbjct: 432 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVP 491
Query: 502 VAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILRKELRARLA 560
VAFV ++NG I+ED++KQ+ISKQV+FYKRI +VFFV++IPK PSGKILRK+LRARLA
Sbjct: 492 VAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLA 546
BLAST of Spo25427.1 vs. ExPASy Swiss-Prot
Match:
4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)
HSP 1 Score: 773.5 bits (1996), Expect = 1.600e-222
Identity = 387/545 (71.01%), Postives = 454/545 (83.30%), Query Frame = 1
Query: 20 DTKE--FIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKTAR 79
DTK+ IFRSKLPDIYIPNHLPLH+YCFEN+ E S CLI+G + +TY +VE +R
Sbjct: 4 DTKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSR 63
Query: 80 QVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQARV 139
+V AG H K G+Q IM+LL NSPEFVF+F+GAS++GA+ST ANP T E+ KQA+
Sbjct: 64 KVAAGLH-KQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 123
Query: 140 SGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGEEDNLPE 199
S A+ I+TQ+ +KV+ Y +N++ +I +D+ +P+ H + V TQ E ++PE
Sbjct: 124 SSAKIIVTQACHVNKVKDYAFENDVKIICIDS-APEGCLHFS------VLTQANEHDIPE 183
Query: 200 ANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVVLCVLPL 259
I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY + +DV+LCVLPL
Sbjct: 184 VEIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPL 243
Query: 260 FHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAKNQDMD 319
FHIYSLNSVLLC LRVGAAIL+M KFDI +EL+Q+YKVTI PFVPPIVLAIAK+ +D
Sbjct: 244 FHIYSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVD 303
Query: 320 RYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPMEV 379
YDLSSVR V+SGAAP+GK+LED VRAK PNAKLGQGYGMTEAGPVLAMCLAFAKEP E+
Sbjct: 304 DYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEI 363
Query: 380 KSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNTIDKEGW 439
KSG+CGTVVRNAE+KIVDP TG SLPRNQ GEICIRG QIMKGYLNDPEAT TIDKEGW
Sbjct: 364 KSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGW 423
Query: 440 LHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVAMQDEAA 499
L+TGDIG++DDDDE+FIVDRLKELIKYKGFQVAPAELE +L+NHP+I DAAVV M+DE A
Sbjct: 424 LYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQA 483
Query: 500 GEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILRKELRAR 559
GEVPVAFV R+NGS I+ED+VK +ISKQV+FYKRI +VFFVD+IPK PSGKILRK+LRA+
Sbjct: 484 GEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAK 540
Query: 560 LANGI 563
LA G+
Sbjct: 544 LAAGL 540
BLAST of Spo25427.1 vs. TAIR (Arabidopsis)
Match:
AT1G51680.1 (4-coumarate:CoA ligase 1)
HSP 1 Score: 750.0 bits (1935), Expect = 1.100e-216
Identity = 369/544 (67.83%), Postives = 441/544 (81.07%), Query Frame = 1
Query: 20 DTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKTARQV 79
+ + IFRSKLPDIYIPNHL LH Y F+N+ E + CLI+GPTG +TY +V +RQ+
Sbjct: 19 NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78
Query: 80 GAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQARVSG 139
A FH KLG+ + V+MLLL N PEFV SFL ASF GA +T ANPF T EI KQA+ S
Sbjct: 79 AANFH-KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 138
Query: 140 AQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGEEDNLPEA- 199
+ I+T++ + DK++P D+ + ++ +D P F ++ + E + ++
Sbjct: 139 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 198
Query: 200 NIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVVLCVLPLF 259
I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYF+ DDV+LCVLP+F
Sbjct: 199 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 258
Query: 260 HIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAKNQDMDR 319
HIY+LNS++LC LRVGAAIL+MPKF+IN+L+EL+Q+ KVT+AP VPPIVLAIAK+ + ++
Sbjct: 259 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 318
Query: 320 YDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPMEVK 379
YDLSS+RVV SGAAP+GK+LEDAV AK PNAKLGQGYGMTEAGPVLAM L FAKEP VK
Sbjct: 319 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 378
Query: 380 SGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNTIDKEGWL 439
SG+CGTVVRNAE+KIVDPDTG SL RNQ GEICIRG QIMKGYLN+P AT TIDK+GWL
Sbjct: 379 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 438
Query: 440 HTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVAMQDEAAG 499
HTGDIG +DDDDE+FIVDRLKELIKYKGFQVAPAELE +LI HP I D AVVAM++EAAG
Sbjct: 439 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 498
Query: 500 EVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILRKELRARL 559
EVPVAFV ++ S++SEDDVKQ++SKQVVFYKRI KVFF +SIPK PSGKILRK+LRA+L
Sbjct: 499 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 558
Query: 560 ANGI 563
ANG+
Sbjct: 559 ANGL 561
BLAST of Spo25427.1 vs. TAIR (Arabidopsis)
Match:
AT3G21240.1 (4-coumarate:CoA ligase 2)
HSP 1 Score: 746.5 bits (1926), Expect = 1.200e-215
Identity = 375/555 (67.57%), Postives = 455/555 (81.98%), Query Frame = 1
Query: 11 DQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYE 70
DQN+ + + + IFRS+LPDIYIPNHLPLH Y FEN+ E + CLI+GPTG +TY
Sbjct: 10 DQNDQKQC--SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYA 69
Query: 71 EVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVE 130
+V T+R++ AG H+ LG+++ V+M+LL NSPE V +FL ASFIGA++T+ANPF T E
Sbjct: 70 DVHVTSRKLAAGLHN-LGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAE 129
Query: 131 IEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQG 190
I KQA+ S A+ I+TQS + DK++ D +++ T D P F ++ TQ
Sbjct: 130 ISKQAKASAAKLIVTQSRYVDKIKNLQNDG----VLIVTTDSDAIPENCLRFSEL--TQS 189
Query: 191 EE---DNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNG 250
EE D++PE I P+DVVALP+SSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYFN
Sbjct: 190 EEPRVDSIPE-KISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNR 249
Query: 251 DDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIV 310
DDV+LCVLP+FHIY+LNS++LC+LRVGA IL+MPKF+I +L+E +Q+ KVT+A VPPIV
Sbjct: 250 DDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIV 309
Query: 311 LAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMC 370
LAIAK+ + ++YDLSSVR+V SGAAP+GK+LEDA+ AK PNAKLGQGYGMTEAGPVLAM
Sbjct: 310 LAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMS 369
Query: 371 LAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEA 430
L FAKEP VKSG+CGTVVRNAE+KI+DPDTG SLPRN+ GEICIRG+QIMKGYLNDP A
Sbjct: 370 LGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLA 429
Query: 431 TTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDA 490
T +TIDK+GWLHTGD+GF+DDDDE+FIVDRLKELIKYKGFQVAPAELE +LI HP I+D
Sbjct: 430 TASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDV 489
Query: 491 AVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSG 550
AVVAM++E AGEVPVAFV R+ S ISED++KQ++SKQVVFYKRI KVFF DSIPK PSG
Sbjct: 490 AVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSG 549
Query: 551 KILRKELRARLANGI 563
KILRK+LRARLANG+
Sbjct: 550 KILRKDLRARLANGL 554
BLAST of Spo25427.1 vs. TAIR (Arabidopsis)
Match:
AT1G65060.1 (4-coumarate:CoA ligase 3)
HSP 1 Score: 683.7 bits (1763), Expect = 9.500e-197
Identity = 352/535 (65.79%), Postives = 424/535 (79.25%), Query Frame = 1
Query: 25 IFRSKLPDIYIPNHLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKTARQVGAGFH 84
IFRSKLPDI IPNHLPLHTYCFE L CLI G TG+ +TY E R+V +G +
Sbjct: 34 IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93
Query: 85 HKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQARVSGAQAIL 144
KLG++KG VIM+LLQNS EFVFSF+GAS IGAVSTTANPF TS E+ KQ + SGA+ I+
Sbjct: 94 -KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153
Query: 145 TQSAFADKVRPYCKDNNIHLIVVDTLSPDDGPHENKYFWDIVGTQGEEDNLPEA-NIIPD 204
T S + DK++ N+ LI D +P EN + + T E + E +I D
Sbjct: 154 THSQYVDKLKNL--GENLTLITTDEPTP-----ENCLPFSTLITDDETNPFQETVDIGGD 213
Query: 205 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFNGDDVVLCVLPLFHIYSL 264
D ALP+SSGTTGLPKGV+LTHKSL+TSVAQQVDG+NPNLY +DV+LCVLPLFHIYSL
Sbjct: 214 DAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSL 273
Query: 265 NSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAKNQDMDRYDLSS 324
NSVLL +LR GA +L+M KF+I L++L+Q+++VTIA VPP+V+A+AKN ++ YDLSS
Sbjct: 274 NSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSS 333
Query: 325 VRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPMEVKSGSCG 384
VR VLSGAAP+GK+L+D++R +LP A LGQGYGMTEAGPVL+M L FAKEP+ KSGSCG
Sbjct: 334 VRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCG 393
Query: 385 TVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTNTIDKEGWLHTGDI 444
TVVRNAELK+V +T SL NQ GEICIRG QIMK YLNDPEAT+ TID+EGWLHTGDI
Sbjct: 394 TVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDI 453
Query: 445 GFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVVAMQDEAAGEVPVA 504
G+VD+DDEIFIVDRLKE+IK+KGFQV PAELE +LINH SI DAAVV DE AGEVPVA
Sbjct: 454 GYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVA 513
Query: 505 FVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKILRKELRARL 559
FV R+NG+ I+E+DVK+Y++KQVVFYKR++KVFFV SIPK PSGKILRK+L+A+L
Sbjct: 514 FVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560
BLAST of Spo25427.1 vs. TAIR (Arabidopsis)
Match:
AT3G21230.1 (4-coumarate:CoA ligase 5)
HSP 1 Score: 675.6 bits (1742), Expect = 2.600e-194
Identity = 343/564 (60.82%), Postives = 435/564 (77.13%), Query Frame = 1
Query: 6 VSNHIDQNEVEGVVDTKEFIFRSKLPDIYIPNHLPLHTYCFENLREHG----SHTCLIDG 65
++ IDQ + E + +FIFRSKLPDI+IPNHLPL Y F+ G S TC+IDG
Sbjct: 10 LTKKIDQEDEEEE-PSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDG 69
Query: 66 PTGRKWTYEEVEKTARQVGAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTT 125
TGR TY +V+ R++ AG H +LG++ G V+MLLL NSPEF SFL +++GAVSTT
Sbjct: 70 ATGRILTYADVQTNMRRIAAGIH-RLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTT 129
Query: 126 ANPFCTSVEIEKQARVSGAQAILTQSAFADKVRPYCKDNNIHLIVVD-------TLSPDD 185
ANPF T EI KQA+ S A+ I+T+ DK+ K++ + ++ +D S DD
Sbjct: 130 ANPFYTQPEIAKQAKASAAKMIITKKCLVDKLTNL-KNDGVLIVCLDDDGDNGVVSSSDD 189
Query: 186 GPHENKYFWDIVGTQGEEDNLPEANIIPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQ 245
G F ++ TQ +E L + I P+D VA+PYSSGTTGLPKGVM+THK LVTS+AQ
Sbjct: 190 GCVS---FTEL--TQADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQ 249
Query: 246 QVDGENPNLYFNGDDVVLCVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQK 305
+VDGENPNL F +DV+LC LP+FHIY+L++++L A+R GAA+L++P+F++N++MEL+Q+
Sbjct: 250 KVDGENPNLNFTANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQR 309
Query: 306 YKVTIAPFVPPIVLAIAKNQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQG 365
YKVT+ P PP+VLA K+ + +RYDLSSVR++LSGAA + K+LEDAVR K PNA GQG
Sbjct: 310 YKVTVVPVAPPVVLAFIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQG 369
Query: 366 YGMTEAGPVLAMCLAFAKEPMEVKSGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRG 425
YGMTE+G V A LAFAK P + KSG+CGTV+RNAE+K+VD +TG SLPRN+ GEIC+RG
Sbjct: 370 YGMTESGTV-AKSLAFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRG 429
Query: 426 SQIMKGYLNDPEATTNTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAEL 485
Q+MKGYLNDPEAT TIDK+GWLHTGDIGFVDDDDEIFIVDRLKELIK+KG+QVAPAEL
Sbjct: 430 HQLMKGYLNDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAEL 489
Query: 486 EGMLINHPSIHDAAVVAMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYK 545
E +LI+HPSI DAAVVAM+DE A EVPVAFVAR+ GSQ++EDDVK Y++KQVV YKRI
Sbjct: 490 EALLISHPSIDDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKM 549
Query: 546 VFFVDSIPKLPSGKILRKELRARL 559
VFF++ IPK SGKILRK+LRA+L
Sbjct: 550 VFFIEVIPKAVSGKILRKDLRAKL 564
BLAST of Spo25427.1 vs. TAIR (Arabidopsis)
Match:
AT4G05160.1 (AMP-dependent synthetase and ligase family protein)
HSP 1 Score: 391.3 bits (1004), Expect = 9.800e-109
Identity = 219/544 (40.26%), Postives = 335/544 (61.58%), Query Frame = 1
Query: 22 KEFIFRSKLPDIYIPN--HLPLHTYCFENLREHGSHTCLIDGPTGRKWTYEEVEKTARQV 81
++ I+RS P + +P + L ++ F N + S + D TG T+ +++ ++
Sbjct: 8 RDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARL 67
Query: 82 GAGFHHKLGLQKGQVIMLLLQNSPEFVFSFLGASFIGAVSTTANPFCTSVEIEKQARVSG 141
GFH +LG++K V+++ NS +F FL + IG V TTANP T E+ KQ + S
Sbjct: 68 AHGFH-RLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSN 127
Query: 142 AQAILTQSAFADKVRPYCKDNNIHLI-VVDTLSPDDGPHENKYFWDIVGTQGEE-DNLPE 201
+ I++ + DK++ + D + L+ DT+ G + +D V E P
Sbjct: 128 PKIIISVNQLFDKIKGF--DLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPF 187
Query: 202 ANIIPDDVVALPYSSGTTGLPKGVMLTH-----KSLVTSVAQQVDGENPNLYFNGDDVVL 261
I D AL YSSGTTG KGV LTH SL+ ++ Q + GE ++G V L
Sbjct: 188 VEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGE-----YHG--VFL 247
Query: 262 CVLPLFHIYSLNSVLLCALRVGAAILVMPKFDINMLMELVQKYKVTIAPFVPPIVLAIAK 321
C LP+FH++ L + L+ G A++ M +F++ ++++ ++K++VT VPP+ LA++K
Sbjct: 248 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 307
Query: 322 NQDMDRYDLSSVRVVLSGAAPMGKDLEDAVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAK 381
+ ++DLSS++ + SGAAP+GKDL + +PN L QGYGMTE ++++ +
Sbjct: 308 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSV-----E 367
Query: 382 EPMEVK--SGSCGTVVRNAELKIVDPDTGCSLPRNQRGEICIRGSQIMKGYLNDPEATTN 441
+P K SGS G + E +IV +TG S P NQ+GEI +RG +MKGYLN+P+AT
Sbjct: 368 DPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKE 427
Query: 442 TIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEGMLINHPSIHDAAVV 501
TIDK+ W+HTGD+G+ ++D +++VDR+KELIKYKGFQVAPAELEG+L++HP I DA V+
Sbjct: 428 TIDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVI 487
Query: 502 AMQDEAAGEVPVAFVARTNGSQISEDDVKQYISKQVVFYKRIYKVFFVDSIPKLPSGKIL 555
DE AGEVP+AFV R+ S I+E D++++I+KQV YKR+ +V F+ +PK +GKIL
Sbjct: 488 PFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKIL 536
The following BLAST results are available for this feature:
