Spo17527.1 (mRNA) | SpinachBase

Overview


Name	Spo17527.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Multiprotein-bridging factor, putative
Location	SpoScf_02492 : 27485 .. 27922 (-)
Sequence length	438

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAACCCGACCAGCTGGCCCAATCTCTCAAGACTGGGAACCCGTCGTCCTCCACAAAAACCGCCCAAAAACCCAAGACCTTCGGGACCCAAAAGCCGTGAACCAAGCTCTACGCTCCGGCAAAGCTGTGGAAACCGTCAAGAAAGCCGTCGCAGGAACTAACAAAAAGGCACCCGCCACCGTCGTCAACGCTCGGAAACTCGATGAGGAGACGGAGCCGGCGGCGCTAGCCAAGGTCGCGGCTGAGGTTCGTCTAGCTATTCAGAAGGCAAGGATTGCGAAGAAGATGAGCCAAGCTGAACTAGCTAAGTTGATCAATGAACGGCCCCAAGTTGTTCAGGAGTATGAGAATGGGAAAGCGGTTCCTAATCATATGGTTCTTGCTAAAATGGAGAGGGTTCTTGGTGTTAAGCTCAGAGGAAAATCTGGAAAATGA

mRNA sequence

ATGCCAACCCGACCAGCTGGCCCAATCTCTCAAGACTGGGAACCCGTCGTCCTCCACAAAAACCGCCCAAAAACCCAAGACCTTCGGGACCCAAAAGCCGTGAACCAAGCTCTACGCTCCGGCAAAGCTGTGGAAACCGTCAAGAAAGCCGTCGCAGGAACTAACAAAAAGGCACCCGCCACCGTCGTCAACGCTCGGAAACTCGATGAGGAGACGGAGCCGGCGGCGCTAGCCAAGGTCGCGGCTGAGGTTCGTCTAGCTATTCAGAAGGCAAGGATTGCGAAGAAGATGAGCCAAGCTGAACTAGCTAAGTTGATCAATGAACGGCCCCAAGTTGTTCAGGAGTATGAGAATGGGAAAGCGGTTCCTAATCATATGGTTCTTGCTAAAATGGAGAGGGTTCTTGGTGTTAAGCTCAGAGGAAAATCTGGAAAATGA

Coding sequence (CDS)

ATGCCAACCCGACCAGCTGGCCCAATCTCTCAAGACTGGGAACCCGTCGTCCTCCACAAAAACCGCCCAAAAACCCAAGACCTTCGGGACCCAAAAGCCGTGAACCAAGCTCTACGCTCCGGCAAAGCTGTGGAAACCGTCAAGAAAGCCGTCGCAGGAACTAACAAAAAGGCACCCGCCACCGTCGTCAACGCTCGGAAACTCGATGAGGAGACGGAGCCGGCGGCGCTAGCCAAGGTCGCGGCTGAGGTTCGTCTAGCTATTCAGAAGGCAAGGATTGCGAAGAAGATGAGCCAAGCTGAACTAGCTAAGTTGATCAATGAACGGCCCCAAGTTGTTCAGGAGTATGAGAATGGGAAAGCGGTTCCTAATCATATGGTTCTTGCTAAAATGGAGAGGGTTCTTGGTGTTAAGCTCAGAGGAAAATCTGGAAAATGA

Protein sequence

MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPATVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGKAVPNHMVLAKMERVLGVKLRGKSGK

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo17527	Spo17527	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo17527.1	Spo17527.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo17527.1.CDS.1	Spo17527.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo17527.1.exon.1	Spo17527.1.exon.1	exon

Homology

[Close]

BLAST of Spo17527.1 vs. NCBI nr
Match: gi|902211792|gb|KNA16812.1| (hypothetical protein SOVF_085800 [Spinacia oleracea])

HSP 1 Score: 270.8 bits (691), Expect = 1.400e-69
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA
Sbjct: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
           TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK
Sbjct: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPNHMVLAKMERVLGVKLRGKSGK
Sbjct: 121 AVPNHMVLAKMERVLGVKLRGKSGK 145

[Close]

BLAST of Spo17527.1 vs. NCBI nr
Match: gi|731353619|ref|XP_010688157.1| (PREDICTED: multiprotein-bridging factor 1c [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 235.0 bits (598), Expect = 8.500e-59
Identity = 125/145 (86.21%), Postives = 133/145 (91.72%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MPTR +GPISQDWEP+VLHK RPK+QDLRD KAVN+ALR+GKAVETVKK VAGTNKK  +
Sbjct: 1   MPTRSSGPISQDWEPIVLHKTRPKSQDLRDRKAVNEALRTGKAVETVKKGVAGTNKKVTS 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
           T VNARKLDEETEPAALAKV++EVRLAIQKARI KKMSQ EL KLINER QVVQEYENGK
Sbjct: 61  TAVNARKLDEETEPAALAKVSSEVRLAIQKARIEKKMSQFELGKLINERTQVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPNHMVLAKMERVLGVKLRGK GK
Sbjct: 121 AVPNHMVLAKMERVLGVKLRGKGGK 145

[Close]

BLAST of Spo17527.1 vs. NCBI nr
Match: gi|224142125|ref|XP_002324409.1| (ethylene-responsive transcriptional coactivator family protein [Populus trichocarpa])

HSP 1 Score: 208.8 bits (530), Expect = 6.500e-51
Identity = 110/145 (75.86%), Postives = 126/145 (86.90%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MP+R AG I QDWEPVV+HK +PK+QDLRDPK VN ALRSG  V+T+KK  AG+NKKA A
Sbjct: 1   MPSRSAGVIKQDWEPVVMHKAKPKSQDLRDPKVVNHALRSGAPVQTIKKFDAGSNKKATA 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
            VVNARKL+EETEPAAL +++ EVR AIQKAR+ KKMSQ ELAKLINE+P+VVQEYENGK
Sbjct: 61  PVVNARKLEEETEPAALDRISTEVRQAIQKARLEKKMSQTELAKLINEQPKVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPN  +LAKMERVLGVKLRGK+GK
Sbjct: 121 AVPNQAILAKMERVLGVKLRGKTGK 145

[Close]

BLAST of Spo17527.1 vs. NCBI nr
Match: gi|743900297|ref|XP_011043445.1| (PREDICTED: multiprotein-bridging factor 1c [Populus euphratica])

HSP 1 Score: 208.0 bits (528), Expect = 1.100e-50
Identity = 110/145 (75.86%), Postives = 126/145 (86.90%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MP+R AG I QDWEPVV+HK +PK+QDLRDPKAVN ALRSG  V+T+KK  AG+NKKA A
Sbjct: 1   MPSRSAGVIKQDWEPVVMHKAKPKSQDLRDPKAVNHALRSGAPVQTIKKFDAGSNKKATA 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
            VV+ARKL+EETEPAAL +++ EVR AIQKAR+ KKMSQ ELAKLINE+P+VVQEYENGK
Sbjct: 61  PVVSARKLEEETEPAALGRISTEVRQAIQKARLEKKMSQTELAKLINEQPKVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPN  +LAKMERVLGVKLRGK GK
Sbjct: 121 AVPNQAILAKMERVLGVKLRGKIGK 145

[Close]

BLAST of Spo17527.1 vs. NCBI nr
Match: gi|719980400|ref|XP_010249785.1| (PREDICTED: multiprotein-bridging factor 1c [Nelumbo nucifera])

HSP 1 Score: 202.6 bits (514), Expect = 4.700e-49
Identity = 115/145 (79.31%), Postives = 125/145 (86.21%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MP+R  G ++QDWEPVVLHK++P  Q LRDPKAVNQALRSG AV+TVKK  AG+NKK PA
Sbjct: 1   MPSRFPGALTQDWEPVVLHKSKPNAQALRDPKAVNQALRSGAAVQTVKKFDAGSNKK-PA 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
           TVVNARKLDE TEPAAL KV+ EVR AIQKAR+AKKMSQAELAKLINER  VVQEYENGK
Sbjct: 61  TVVNARKLDEGTEPAALDKVSTEVRQAIQKARLAKKMSQAELAKLINERTLVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPN  VLAKME+VL VKLRGKS K
Sbjct: 121 AVPNQAVLAKMEKVLKVKLRGKSHK 144

[Close]

BLAST of Spo17527.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RD99_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_085800 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 9.800e-70
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA
Sbjct: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
           TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK
Sbjct: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPNHMVLAKMERVLGVKLRGKSGK
Sbjct: 121 AVPNHMVLAKMERVLGVKLRGKSGK 145

[Close]

BLAST of Spo17527.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BP57_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g195890 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.900e-59
Identity = 125/145 (86.21%), Postives = 133/145 (91.72%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MPTR +GPISQDWEP+VLHK RPK+QDLRD KAVN+ALR+GKAVETVKK VAGTNKK  +
Sbjct: 1   MPTRSSGPISQDWEPIVLHKTRPKSQDLRDRKAVNEALRTGKAVETVKKGVAGTNKKVTS 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
           T VNARKLDEETEPAALAKV++EVRLAIQKARI KKMSQ EL KLINER QVVQEYENGK
Sbjct: 61  TAVNARKLDEETEPAALAKVSSEVRLAIQKARIEKKMSQFELGKLINERTQVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPNHMVLAKMERVLGVKLRGK GK
Sbjct: 121 AVPNHMVLAKMERVLGVKLRGKGGK 145

[Close]

BLAST of Spo17527.1 vs. UniProtKB/TrEMBL
Match: B9ILK1_POPTR (Ethylene-responsive transcriptional coactivator family protein OS=Populus trichocarpa GN=POPTR_0018s06080g PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 4.600e-51
Identity = 110/145 (75.86%), Postives = 126/145 (86.90%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MP+R AG I QDWEPVV+HK +PK+QDLRDPK VN ALRSG  V+T+KK  AG+NKKA A
Sbjct: 1   MPSRSAGVIKQDWEPVVMHKAKPKSQDLRDPKVVNHALRSGAPVQTIKKFDAGSNKKATA 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
            VVNARKL+EETEPAAL +++ EVR AIQKAR+ KKMSQ ELAKLINE+P+VVQEYENGK
Sbjct: 61  PVVNARKLEEETEPAALDRISTEVRQAIQKARLEKKMSQTELAKLINEQPKVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPN  +LAKMERVLGVKLRGK+GK
Sbjct: 121 AVPNQAILAKMERVLGVKLRGKTGK 145

[Close]

BLAST of Spo17527.1 vs. UniProtKB/TrEMBL
Match: A0A0D2SNV8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G069500 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 4.300e-49
Identity = 109/145 (75.17%), Postives = 123/145 (84.83%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MPTR  G ++QDWEPVVLHK++PK QDLRDPKAVN+ALRSG  V+T+KK  AG+NKK   
Sbjct: 20  MPTRFGGAVTQDWEPVVLHKSKPKAQDLRDPKAVNRALRSGAPVQTIKKFDAGSNKKTAG 79

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
            VVNARKLDE TEPAAL +V  +VR  IQKAR+ KK+SQAELAKLINE+ +VVQEYENGK
Sbjct: 80  PVVNARKLDEGTEPAALDRVPTDVRQTIQKARLEKKLSQAELAKLINEQLKVVQEYENGK 139

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPN  VLAKMERVLGVKLRGKSGK
Sbjct: 140 AVPNQAVLAKMERVLGVKLRGKSGK 164

[Close]

BLAST of Spo17527.1 vs. UniProtKB/TrEMBL
Match: S4SF23_GOSHI (Multiprotein bridging factor 1 transcriptional coactivator OS=Gossypium hirsutum GN=MBF1c PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 9.500e-49
Identity = 109/145 (75.17%), Postives = 122/145 (84.14%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MPT   G ++QDWEPVVLHK++PK QDLRDPKAVNQALRSG  V+T+KK  AG+NKK   
Sbjct: 1   MPTGFGGAVTQDWEPVVLHKSKPKAQDLRDPKAVNQALRSGAPVQTIKKFDAGSNKKTAG 60

Query: 61  TVVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGK 120
            VVNARKLDE TEPAAL +V  +VR  IQKAR+ KK+SQAELAKLINE+ +VVQEYENGK
Sbjct: 61  PVVNARKLDEGTEPAALDRVPTDVRQTIQKARLEKKLSQAELAKLINEQLKVVQEYENGK 120

Query: 121 AVPNHMVLAKMERVLGVKLRGKSGK 146
           AVPN  VLAKMERVLGVKLRGKSGK
Sbjct: 121 AVPNQAVLAKMERVLGVKLRGKSGK 145

[Close]

BLAST of Spo17527.1 vs. ExPASy Swiss-Prot
Match: MBF1C_ARATH (Multiprotein-bridging factor 1c OS=Arabidopsis thaliana GN=MBF1C PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 4.700e-49
Identity = 107/148 (72.30%), Postives = 125/148 (84.46%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MP+R  G ++QDWEPVVLHK++ K+QDLRDPKAVN ALR+G AV+TVKK  AG+NKK  +
Sbjct: 1   MPSRYPGAVTQDWEPVVLHKSKQKSQDLRDPKAVNAALRNGVAVQTVKKFDAGSNKKGKS 60

Query: 61  T---VVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYE 120
           T   V+N +KL+EETEPAA+ +V AEVRL IQKAR+ KKMSQA+LAK INER QVVQEYE
Sbjct: 61  TAVPVINTKKLEEETEPAAMDRVKAEVRLMIQKARLEKKMSQADLAKQINERTQVVQEYE 120

Query: 121 NGKAVPNHMVLAKMERVLGVKLRGKSGK 146
           NGKAVPN  VLAKME+VLGVKLRGK GK
Sbjct: 121 NGKAVPNQAVLAKMEKVLGVKLRGKIGK 148

[Close]

BLAST of Spo17527.1 vs. ExPASy Swiss-Prot
Match: MBF1B_ARATH (Multiprotein-bridging factor 1b OS=Arabidopsis thaliana GN=MBF1B PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.200e-32
Identity = 75/137 (54.74%), Postives = 101/137 (73.72%), Query Frame = 1


		  
Query: 7   GPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA-TVVNA 66
           GPI+QDWEPVV+ K  P     RD K VN A RSG  +ETV+K  AG+NK A + T +N 
Sbjct: 5   GPITQDWEPVVIRKRAPNAAAKRDEKTVNAARRSGADIETVRKFNAGSNKAASSGTSLNT 64

Query: 67  RKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGKAVPNH 126
           +KLD++TE  +  +V  E++ AI +AR  KK++Q++LA LINE+PQV+QEYE+GKA+PN 
Sbjct: 65  KKLDDDTENLSHDRVPTELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQ 124

Query: 127 MVLAKMERVLGVKLRGK 143
            +L+K+ER LG KLRGK
Sbjct: 125 QILSKLERALGAKLRGK 141

[Close]

BLAST of Spo17527.1 vs. ExPASy Swiss-Prot
Match: MBF1A_ARATH (Multiprotein-bridging factor 1a OS=Arabidopsis thaliana GN=MBF1A PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 5.700e-31
Identity = 73/137 (53.28%), Postives = 99/137 (72.26%), Query Frame = 1


		  
Query: 7   GPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA-TVVNA 66
           GPI+QDWEPVV+ K        RD K VN A RSG  +ETV+K  AGTNK A + T +N 
Sbjct: 5   GPITQDWEPVVIRKKPANAAAKRDEKTVNAARRSGADIETVRKFNAGTNKAASSGTSLNT 64

Query: 67  RKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGKAVPNH 126
           + LD++TE     +V  E++ AI +AR  KK++Q++LA++INE+PQV+QEYE+GKA+PN 
Sbjct: 65  KMLDDDTENLTHERVPTELKKAIMQARTDKKLTQSQLAQIINEKPQVIQEYESGKAIPNQ 124

Query: 127 MVLAKMERVLGVKLRGK 143
            +L+K+ER LG KLRGK
Sbjct: 125 QILSKLERALGAKLRGK 141

[Close]

BLAST of Spo17527.1 vs. ExPASy Swiss-Prot
Match: EDF1_DANRE (Endothelial differentiation-related factor 1 homolog OS=Danio rerio GN=edf1 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.600e-20
Identity = 59/131 (45.04%), Postives = 82/131 (62.60%), Query Frame = 1


		  
Query: 12  DWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPATVVNARKLDEE 71
           DW+ V + + +      +  +AV  A R G+AVET KK  AG NK+   T  N  KLD E
Sbjct: 5   DWDTVTVLRKKGSAAQSKSKQAVTAAQRKGEAVETSKKWAAGQNKQHVVTK-NTAKLDRE 64

Query: 72  TEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGKAVPNHMVLAKM 131
           TE  +  +V  EV   IQ+ R  K ++Q +LA  INE+PQ++ EYE GKA+PN+ V+ K+
Sbjct: 65  TEELSHQRVPLEVGKVIQQGRQNKGLTQKDLATKINEKPQIIAEYECGKAIPNNQVMGKI 124

Query: 132 ERVLGVKLRGK 143
           ER +G+KLRGK
Sbjct: 125 ERAIGLKLRGK 134

[Close]

BLAST of Spo17527.1 vs. ExPASy Swiss-Prot
Match: EDF1_XENLA (Endothelial differentiation-related factor 1 homolog OS=Xenopus laevis GN=edf1 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 6.000e-20
Identity = 63/132 (47.73%), Postives = 82/132 (62.12%), Query Frame = 1


		  
Query: 12  DWEPV-VLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPATVVNARKLDE 71
           DW+ V VL K  P     +  +A+  A R G+ VET KK  AG NK+   T  N  KLD 
Sbjct: 5   DWDTVTVLRKKGPTAAQAKSKQAITAAQRRGEEVETSKKWSAGQNKQHTITR-NTAKLDR 64

Query: 72  ETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGKAVPNHMVLAK 131
           ETE     +V  EV   IQ+ R  K M+Q +LA  INE+PQV+ +YE GKA+PN+ V+ K
Sbjct: 65  ETEELHHDRVPLEVGKVIQQGRQGKGMTQKDLATKINEKPQVIADYECGKAIPNNQVMGK 124

Query: 132 MERVLGVKLRGK 143
           +ERV+G+KLRGK
Sbjct: 125 IERVIGLKLRGK 135

[Close]

BLAST of Spo17527.1 vs. TAIR (Arabidopsis)
Match: AT3G24500.1 (multiprotein bridging factor 1C)

HSP 1 Score: 195.3 bits (495), Expect = 2.600e-50
Identity = 107/148 (72.30%), Postives = 125/148 (84.46%), Query Frame = 1


		  
Query: 1   MPTRPAGPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA 60
           MP+R  G ++QDWEPVVLHK++ K+QDLRDPKAVN ALR+G AV+TVKK  AG+NKK  +
Sbjct: 1   MPSRYPGAVTQDWEPVVLHKSKQKSQDLRDPKAVNAALRNGVAVQTVKKFDAGSNKKGKS 60

Query: 61  T---VVNARKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYE 120
           T   V+N +KL+EETEPAA+ +V AEVRL IQKAR+ KKMSQA+LAK INER QVVQEYE
Sbjct: 61  TAVPVINTKKLEEETEPAAMDRVKAEVRLMIQKARLEKKMSQADLAKQINERTQVVQEYE 120

Query: 121 NGKAVPNHMVLAKMERVLGVKLRGKSGK 146
           NGKAVPN  VLAKME+VLGVKLRGK GK
Sbjct: 121 NGKAVPNQAVLAKMEKVLGVKLRGKIGK 148

[Close]

BLAST of Spo17527.1 vs. TAIR (Arabidopsis)
Match: AT3G58680.1 (multiprotein bridging factor 1B)

HSP 1 Score: 138.7 bits (348), Expect = 2.900e-33
Identity = 75/137 (54.74%), Postives = 101/137 (73.72%), Query Frame = 1


		  
Query: 7   GPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA-TVVNA 66
           GPI+QDWEPVV+ K  P     RD K VN A RSG  +ETV+K  AG+NK A + T +N 
Sbjct: 5   GPITQDWEPVVIRKRAPNAAAKRDEKTVNAARRSGADIETVRKFNAGSNKAASSGTSLNT 64

Query: 67  RKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGKAVPNH 126
           +KLD++TE  +  +V  E++ AI +AR  KK++Q++LA LINE+PQV+QEYE+GKA+PN 
Sbjct: 65  KKLDDDTENLSHDRVPTELKKAIMQARGEKKLTQSQLAHLINEKPQVIQEYESGKAIPNQ 124

Query: 127 MVLAKMERVLGVKLRGK 143
            +L+K+ER LG KLRGK
Sbjct: 125 QILSKLERALGAKLRGK 141

[Close]

BLAST of Spo17527.1 vs. TAIR (Arabidopsis)
Match: AT2G42680.1 (multiprotein bridging factor 1A)

HSP 1 Score: 135.2 bits (339), Expect = 3.200e-32
Identity = 73/137 (53.28%), Postives = 99/137 (72.26%), Query Frame = 1


		  
Query: 7   GPISQDWEPVVLHKNRPKTQDLRDPKAVNQALRSGKAVETVKKAVAGTNKKAPA-TVVNA 66
           GPI+QDWEPVV+ K        RD K VN A RSG  +ETV+K  AGTNK A + T +N 
Sbjct: 5   GPITQDWEPVVIRKKPANAAAKRDEKTVNAARRSGADIETVRKFNAGTNKAASSGTSLNT 64

Query: 67  RKLDEETEPAALAKVAAEVRLAIQKARIAKKMSQAELAKLINERPQVVQEYENGKAVPNH 126
           + LD++TE     +V  E++ AI +AR  KK++Q++LA++INE+PQV+QEYE+GKA+PN 
Sbjct: 65  KMLDDDTENLTHERVPTELKKAIMQARTDKKLTQSQLAQIINEKPQVIQEYESGKAIPNQ 124

Query: 127 MVLAKMERVLGVKLRGK 143
            +L+K+ER LG KLRGK
Sbjct: 125 QILSKLERALGAKLRGK 141

The following BLAST results are available for this feature:

BLAST of Spo17527.1 vs. NCBI nr
BLAST of Spo17527.1 vs. UniProtKB/TrEMBL
BLAST of Spo17527.1 vs. ExPASy Swiss-Prot
BLAST of Spo17527.1 vs. TAIR (Arabidopsis)

BLAST of Spo17527.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902211792\|gb\|KNA16812.1\|	1.4e-69	100.	hypothetical protein SOVF_0858...	[more]
gi\|731353619\|ref\|XP_010688157.1\|	8.5e-59	86.2	PREDICTED: multiprotein-bridgi...	[more]
gi\|224142125\|ref\|XP_002324409.1\|	6.5e-51	75.8	ethylene-responsive transcript...	[more]
gi\|743900297\|ref\|XP_011043445.1\|	1.1e-50	75.8	PREDICTED: multiprotein-bridgi...	[more]
gi\|719980400\|ref\|XP_010249785.1\|	4.7e-49	79.3	PREDICTED: multiprotein-bridgi...	[more]

BLAST of Spo17527.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RD99_SPIOL	9.8e-70	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8BP57_BETVU	5.9e-59	86.2	Uncharacterized protein OS=Bet...	[more]
B9ILK1_POPTR	4.6e-51	75.8	Ethylene-responsive transcript...	[more]
A0A0D2SNV8_GOSRA	4.3e-49	75.1	Uncharacterized protein OS=Gos...	[more]
S4SF23_GOSHI	9.5e-49	75.1	Multiprotein bridging factor 1...	[more]

BLAST of Spo17527.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MBF1C_ARATH	4.7e-49	72.3	Multiprotein-bridging factor 1...	[more]
MBF1B_ARATH	5.2e-32	54.7	Multiprotein-bridging factor 1...	[more]
MBF1A_ARATH	5.7e-31	53.2	Multiprotein-bridging factor 1...	[more]
EDF1_DANRE	4.6e-20	45.0	Endothelial differentiation-re...	[more]
EDF1_XENLA	6.0e-20	47.7	Endothelial differentiation-re...	[more]

BLAST of Spo17527.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT3G24500.1	2.6e-50	72.3	multiprotein bridging factor 1...	[more]
AT3G58680.1	2.9e-33	54.7	multiprotein bridging factor 1...	[more]
AT2G42680.1	3.2e-32	53.2	multiprotein bridging factor 1...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001387	Cro/C1-type helix-turn-helix domain	PFAM	PF01381	HTH_3	coord: 88..139 score: 2.6
IPR001387	Cro/C1-type helix-turn-helix domain	SMART	SM00530	mbf_short4	coord: 87..142 score: 6.1
IPR001387	Cro/C1-type helix-turn-helix domain	PROFILE	PS50943	HTH_CROC1	coord: 88..142 score: 1
IPR010982	Lambda repressor-like, DNA-binding domain	GENE3D	1.10.260.40		coord: 87..139 score: 3.4
IPR010982	Lambda repressor-like, DNA-binding domain	unknown	SSF47413	lambda repressor-like DNA-binding domains	coord: 86..139 score: 9.01
IPR013729	Multiprotein bridging factor 1, N-terminal	PFAM	PF08523	MBF1	coord: 11..80 score: 5.5
None	No IPR available	unknown	Coil	Coil	coord: 88..108 scor
None	No IPR available	PANTHER	PTHR10245	ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1	coord: 54..142 score: 5.8
None	No IPR available	PANTHER	PTHR10245:SF33	SUBFAMILY NOT NAMED	coord: 54..142 score: 5.8

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009408	response to heat
biological_process	GO:0009873	ethylene-activated signaling pathway
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006457	protein folding
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0034976	response to endoplasmic reticulum stress
biological_process	GO:0009644	response to high light intensity
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009414	response to water deprivation
cellular_component	GO:0005667	transcription factor complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003713	transcription coactivator activity