Spo21342 (gene) | SpinachBase

Overview


Name	Spo21342
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Metallothionein type I
Location	SpoScf_00928 : 235384 .. 237118 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGAGGACAAGAGTCGTAGACCATTTACCATTTGAAAACATTAACACAAAGATTGAAGATTCGTTTTGGATGGTTCACTTGTTAAAACGCATTTTATTAGGGCCTCCCAAATTGCATGGTATTATTCCTTTGGGCCCACAACTCAAAACCTACGTAGCTCTTCCTCATCTATAAATAGCAACCAATATTGCACCAATTAAAATCCACAACAAACTCAATCAACAAATCATCTATCTTATTAAGCTTAATTAATCTCTTCAAGTTCATATCCAATCTCCCTAATCAACATGTCTGGTCATTGTGGAAACTGCAACTGTGCTGACAACACCCAGTGTGTGTAAGTGCCTCTTAATTCCAAACATTTCCATGCATTTATACTTTATTGTTATTGATCAATTGATTATTACAGCGAATTGTTTAGGATTTACTATATACGTATTTGATTTCAAGTATATGTATTCCTAATTTACTTAGGAAGTTGTTTAGTTCTAAGAAAAGAGGATAATTAAGGAGTTTCACGTGATATGTGTACATAATAATAGTGTAACATGATAATAATACGAGACATCATATGTAAAGAAATGAAAAATGACAGAAAAAATGTAACTAGCTAGCTACGGAGTATAATAAAATGGAAAAGGGGGTTTAGCCAAAAGGAACTTGAGAAGGCGAGAACTGACCTTAATCGAACCTGAAATTATCTTACATGAACTCGATTTGACACGAATTTAGTTCAATGTGGTTGCTAATATTCGTCAAATTTGCACTTTTTGCAGGAACAAGGGAAACCAAATTGGCATGATTGAGACTGAGACCATGTAAGTTCCACAATCCATGTAACAAACGTACTTAATTATGAATTACTACGTATGTTATAATATAAATCAAATATTTGTTTCAATTCTTTCTAAGGAAAAATACACAAAGGTTAAATAAATATATTATTATTAATTATATATATTTGGCGATGGTTTTGCAGCTACTACGATGGTATGATGGAAGGCGGTAACTGTGGTGGCTGCAAGTGCGGCGCCGGCTGCGCATGCACCGACTGCACCTGCTGCACTCACTAAGTAACGAAGCGCCCTCGTTAACTCGAAGGCCTTACTCATATTATCTACGTAGTACTTCGTATTTAATATGAGCTTCGTCTAACTACTACTTTATTATGTTACCTTATACATTATGTATAAGATGAATTAATTATTATTCGAATAATTTTGTTATTGTCACTGCTATGTAATTTGGCCTCTGTGCCTAGTTATATTGCTATCAATATATTGCATCGATTTTATAATTACCTATTAATCCGTCTCATTCTTTCTAGTATTTTATCATATATTTGTGTGCATTAGACTTTGGCCGTCCATAAACTTGTCATTGGTACACAAGATCTCATTCGCACACAACAAAAAAGTTTTATGAAGATCTCTTTAAGATGCAACGCGCGAGACAACTAATTAACTTAGCACGGTAACTCAAATAAACCAAAGTTAACCAACCAACACTCTGATTATCAAACAAGTCTATACCGTAACAAGAAATGGGTTGAAGTAAGCGAACACCGAAGCCAGTGGCAGATGTTCCTTGTGAGTTGTGAGTATGGTGGGCCAGACCCCAGATAAAAAATTTCGTAGTGTACTTTAAGTTACGACTCACCTAAAATTGATGTAAAATTGTATAAGTTGTGTACTATGTTACTTATAATTAATTTTACTATTATCCCCTCG

mRNA sequence

ATGGAGGGAGGACAAGAGTCTTCATATCCAATCTCCCTAATCAACATGTCTGGTCATTGTGGAAACTGCAACTGTGCTGACAACACCCAGTGTGTGAACAAGGGAAACCAAATTGGCATGATTGAGACTGAGACCATCTACTACGATGGTATGATGGAAGGCGGTAACTGTGGTGGCTGCAAGTGCGGCGCCGGCTGCGCATGCACCGACTGCACCTGCTGCACTCACTAAGTAACGAAGCGCCCTCGTTAACTCGAAGGCCTTACTCATATTATCTACGTAGTACTTCGTATTTAATATGAGCTTCGTCTAACTACTACTTTATTATGTTACCTTATACATTATGTATAAGATGAATTAATTATTATTCGAATAATTTTGTTATTGTCACTGCTATGTAATTTGGCCTCTGTGCCTAGTTATATTGCTATCAATATATTGCATCGATTTTATAATTACCTATTAATCCGTCTCATTCTTTCTAGTATTTTATCATATATTTGTGTGCATTAGACTTTGGCCGTCCATAAACTTGTCATTGGTACACAAGATCTCATTCGCACACAACAAAAAAGTTTTATGAAGATCTCTTTAAGATGCAACGCGCGAGACAACTAATTAACTTAGCACGGTAACTCAAATAAACCAAAGTTAACCAACCAACACTCTGATTATCAAACAAGTCTATACCGTAACAAGAAATGGGTTGAAGTAAGCGAACACCGAAGCCAGTGGCAGATGTTCCTTGTGAGTTGTGAGTATGGTGGGCCAGACCCCAGATAAAAAATTTCGTAGTGTACTTTAAGTTACGACTCACCTAAAATTGATGTAAAATTGTATAAGTTGTGTACTATGTTACTTATAATTAATTTTACTATTATCCCCTCG

Coding sequence (CDS)

ATGGAGGGAGGACAAGAGTCTTCATATCCAATCTCCCTAATCAACATGTCTGGTCATTGTGGAAACTGCAACTGTGCTGACAACACCCAGTGTGTGAACAAGGGAAACCAAATTGGCATGATTGAGACTGAGACCATCTACTACGATGGTATGATGGAAGGCGGTAACTGTGGTGGCTGCAAGTGCGGCGCCGGCTGCGCATGCACCGACTGCACCTGCTGCACTCACTAA

Protein sequence

MEGGQESSYPISLINMSGHCGNCNCADNTQCVNKGNQIGMIETETIYYDGMMEGGNCGGCKCGAGCACTDCTCCTH

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo21342.1	Spo21342.1	mRNA

Homology

[Close]

BLAST of Spo21342.1 vs. NCBI nr
Match: gi|902190953|gb|KNA11734.1| (hypothetical protein SOVF_132380 [Spinacia oleracea])

HSP 1 Score: 141.4 bits (355), Expect = 6.700e-31
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIGMIETETIYYDGMMEGGNCGGCKCGAGCACTDCTCCT 75
          MSGHCGNCNCADNTQCVNKGNQIGMIETETIYYDGMMEGGNCGGCKCGAGCACTDCTCCT
Sbjct: 1  MSGHCGNCNCADNTQCVNKGNQIGMIETETIYYDGMMEGGNCGGCKCGAGCACTDCTCCT 60

Query: 76 H 77
          H
Sbjct: 61 H 61

[Close]

BLAST of Spo21342.1 vs. NCBI nr
Match: gi|870845222|gb|KMS97998.1| (hypothetical protein BVRB_4g096800 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 94.7 bits (234), Expect = 7.200e-17
Identity = 44/65 (67.69%), Postives = 50/65 (76.92%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIG--MIETETIYYDGMM--EGGNCGGCKCGAGCACTDC 75
          MSG CGNCNCAD +QCV KGNQ G  +IE E   +DGM+  EGGNC  CKCG+GCACT C
Sbjct: 1  MSGGCGNCNCADQSQCVKKGNQYGFDIIENENNNFDGMVMEEGGNCDNCKCGSGCACTSC 60

Query: 76 TCCTH 77
          TCC+H
Sbjct: 61 TCCSH 65

[Close]

BLAST of Spo21342.1 vs. NCBI nr
Match: gi|2754853|gb|AAB95220.1| (metallothionein type I [Fritillaria agrestis])

HSP 1 Score: 69.7 bits (169), Expect = 2.500e-9
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIGMI--ETETIYYDGMME-GGNCGGCKCGAGCACTDCT 74
          MS  CGNC+CAD  QCV K N  G++  ETE  Y+DGMM+   +   CKCG+ C+CTDC 
Sbjct: 1  MSSTCGNCDCADKNQCVKKSNSFGVVIMETEKSYFDGMMDVAEHDPKCKCGSSCSCTDCN 60

[Close]

BLAST of Spo21342.1 vs. NCBI nr
Match: gi|257219675|gb|ACV51811.1| (metallothionein type 3 [Typha angustifolia])

HSP 1 Score: 69.3 bits (168), Expect = 3.200e-9
Identity = 34/59 (57.63%), Postives = 39/59 (66.10%), Query Frame = 1


		  
Query: 20 CGNCNCADNTQCVNKGNQIG--MIETETIYYDGMME---GGNCGGCKCGAGCACTDCTC 74
          CGNC+CAD +QCV KGN  G  +IETE  Y+DG+ E     N G CKCG  CAC DC C
Sbjct: 4  CGNCDCADKSQCVKKGNSYGVEIIETEKSYHDGVFEVAAAKNEGNCKCGPSCACVDCHC 62

[Close]

BLAST of Spo21342.1 vs. NCBI nr
Match: gi|76496238|gb|ABA43635.1| (metallothionein-like protein [Metroxylon sagu])

HSP 1 Score: 68.9 bits (167), Expect = 4.200e-9
Identity = 35/60 (58.33%), Postives = 40/60 (66.67%), Query Frame = 1


		  
Query: 20 CGNCNCADNTQCVNKGNQIG--MIETETIYYDGMME----GGNCGGCKCGAGCACTDCTC 74
          CGNC+CAD +QCV KGN  G  +IETE  Y D ++E      N G CKCGA CAC DCTC
Sbjct: 4  CGNCDCADKSQCVKKGNSYGIEIIETEKSYVDHVVEAPAAAKNEGECKCGASCACADCTC 63

[Close]

BLAST of Spo21342.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QWT4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_132380 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 4.700e-31
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIGMIETETIYYDGMMEGGNCGGCKCGAGCACTDCTCCT 75
          MSGHCGNCNCADNTQCVNKGNQIGMIETETIYYDGMMEGGNCGGCKCGAGCACTDCTCCT
Sbjct: 1  MSGHCGNCNCADNTQCVNKGNQIGMIETETIYYDGMMEGGNCGGCKCGAGCACTDCTCCT 60

Query: 76 H 77
          H
Sbjct: 61 H 61

[Close]

BLAST of Spo21342.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BD79_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g096800 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 5.000e-17
Identity = 44/65 (67.69%), Postives = 50/65 (76.92%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIG--MIETETIYYDGMM--EGGNCGGCKCGAGCACTDC 75
          MSG CGNCNCAD +QCV KGNQ G  +IE E   +DGM+  EGGNC  CKCG+GCACT C
Sbjct: 1  MSGGCGNCNCADQSQCVKKGNQYGFDIIENENNNFDGMVMEEGGNCDNCKCGSGCACTSC 60

Query: 76 TCCTH 77
          TCC+H
Sbjct: 61 TCCSH 65

[Close]

BLAST of Spo21342.1 vs. UniProtKB/TrEMBL
Match: B9TSW7_9LILI (Metallothionein-like protein OS=Lilium formosanum GN=MT1 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.700e-9
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIGMI--ETETIYYDGMME-GGNCGGCKCGAGCACTDCT 74
          MS  CGNC+CAD  QCV K N  G++  ETE  Y+DGMM+   +   CKCG+ C+CTDC 
Sbjct: 1  MSSTCGNCDCADKNQCVKKSNSFGVVIMETEKSYFDGMMDVAEHDPKCKCGSSCSCTDCN 60

[Close]

BLAST of Spo21342.1 vs. UniProtKB/TrEMBL
Match: O49126_FRIAG (Metallothionein type I OS=Fritillaria agrestis GN=MET PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.700e-9
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIGMI--ETETIYYDGMME-GGNCGGCKCGAGCACTDCT 74
          MS  CGNC+CAD  QCV K N  G++  ETE  Y+DGMM+   +   CKCG+ C+CTDC 
Sbjct: 1  MSSTCGNCDCADKNQCVKKSNSFGVVIMETEKSYFDGMMDVAEHDPKCKCGSSCSCTDCN 60

[Close]

BLAST of Spo21342.1 vs. UniProtKB/TrEMBL
Match: Q3HR41_METSA (Metallothionein-like protein OS=Metroxylon sagu GN=MET PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 3.000e-9
Identity = 35/60 (58.33%), Postives = 40/60 (66.67%), Query Frame = 1


		  
Query: 20 CGNCNCADNTQCVNKGNQIG--MIETETIYYDGMME----GGNCGGCKCGAGCACTDCTC 74
          CGNC+CAD +QCV KGN  G  +IETE  Y D ++E      N G CKCGA CAC DCTC
Sbjct: 4  CGNCDCADKSQCVKKGNSYGIEIIETEKSYVDHVVEAPAAAKNEGECKCGASCACADCTC 63

[Close]

BLAST of Spo21342.1 vs. ExPASy Swiss-Prot
Match: MT3A_ORYSJ (Metallothionein-like protein 3A OS=Oryza sativa subsp. japonica GN=MT3A PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.500e-11
Identity = 30/60 (50.00%), Postives = 39/60 (65.00%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIGMI--ETETIYYDGMMEGGNCGGCKCGAGCACTDCTC 74
          MS  CGNC+CAD +QCV KG   G++  E E  +++ +  G   GGCKCG  C+CTDC C
Sbjct: 1  MSDKCGNCDCADKSQCVKKGTSYGVVIVEAEKSHFEEVAAGEENGGCKCGTSCSCTDCKC 60

[Close]

BLAST of Spo21342.1 vs. ExPASy Swiss-Prot
Match: MT3A_ORYSI (Metallothionein-like protein 3A OS=Oryza sativa subsp. indica GN=MT3A PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.500e-11
Identity = 30/60 (50.00%), Postives = 39/60 (65.00%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIGMI--ETETIYYDGMMEGGNCGGCKCGAGCACTDCTC 74
          MS  CGNC+CAD +QCV KG   G++  E E  +++ +  G   GGCKCG  C+CTDC C
Sbjct: 1  MSDKCGNCDCADKSQCVKKGTSYGVVIVEAEKSHFEEVAAGEENGGCKCGTSCSCTDCKC 60

[Close]

BLAST of Spo21342.1 vs. ExPASy Swiss-Prot
Match: MT3_ACTDE (Metallothionein-like protein type 3 OS=Actinidia deliciosa GN=pKIWI503 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.700e-10
Identity = 30/62 (48.39%), Postives = 41/62 (66.13%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQIGMIETETIYYDGMMEG----GNCGGCKCGAGCACTDC 74
          MS  CGNC+CAD++QCV KGN I ++ET+  Y + ++ G     + G CKCG  C C +C
Sbjct: 1  MSDKCGNCDCADSSQCVKKGNSIDIVETDKSYIEDVVMGVPAAESGGKCKCGTSCPCVNC 60

[Close]

BLAST of Spo21342.1 vs. ExPASy Swiss-Prot
Match: MT3_MUSAC (Metallothionein-like protein type 3 OS=Musa acuminata PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 8.500e-10
Identity = 31/60 (51.67%), Postives = 37/60 (61.67%), Query Frame = 1


		  
Query: 20 CGNCNCADNTQCVNKGNQIG--MIETETIYYDGMMEGGNC----GGCKCGAGCACTDCTC 74
          CGNC+C D +QCV KGN  G  ++ETE  Y D ++         G CKCGA CACTDC C
Sbjct: 4  CGNCDCVDKSQCVKKGNSYGIDIVETEKSYVDEVIVAAEAAEHDGKCKCGAACACTDCKC 63

[Close]

BLAST of Spo21342.1 vs. ExPASy Swiss-Prot
Match: MT3_MALDO (Metallothionein-like protein type 3 OS=Malus domestica GN=MT2 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.500e-9
Identity = 32/65 (49.23%), Postives = 41/65 (63.08%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGN--QIGMIETE-----TIYYDGMMEGGNCGGCKCGAGCAC 74
          MSG C NC+CAD+TQCV KGN   + ++ETE     T++ D      + G CKCG GC+C
Sbjct: 1  MSGKCDNCDCADSTQCVKKGNSYDLVIVETENRSMDTVFVDA-PAAEHDGKCKCGTGCSC 60

[Close]

BLAST of Spo21342.1 vs. TAIR (Arabidopsis)
Match: AT3G15353.1 (metallothionein 3)

HSP 1 Score: 60.8 bits (146), Expect = 4.100e-10
Identity = 30/68 (44.12%), Postives = 41/68 (60.29%), Query Frame = 1


		  
Query: 16 MSGHCGNCNCADNTQCVNKGNQ--IGMIETETIYYDGMM-------EGGNCGGCKCGAGC 75
          MS +CG+C+CAD TQCV KG      ++ET+  Y + M+          NC  CKCG+ C
Sbjct: 1  MSSNCGSCDCADKTQCVKKGTSYTFDIVETQESYKEAMIMDVGAEENNANC-KCKCGSSC 60

The following BLAST results are available for this feature:

BLAST of Spo21342.1 vs. NCBI nr
BLAST of Spo21342.1 vs. UniProtKB/TrEMBL
BLAST of Spo21342.1 vs. ExPASy Swiss-Prot
BLAST of Spo21342.1 vs. TAIR (Arabidopsis)

BLAST of Spo21342.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902190953\|gb\|KNA11734.1\|	6.7e-31	100.	hypothetical protein SOVF_1323...	[more]
gi\|870845222\|gb\|KMS97998.1\|	7.2e-17	67.6	hypothetical protein BVRB_4g09...	[more]
gi\|2754853\|gb\|AAB95220.1\|	2.5e-9	54.1	metallothionein type I [Fritil...	[more]
gi\|257219675\|gb\|ACV51811.1\|	3.2e-9	57.6	metallothionein type 3 [Typha ...	[more]
gi\|76496238\|gb\|ABA43635.1\|	4.2e-9	58.3	metallothionein-like protein [...	[more]

BLAST of Spo21342.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QWT4_SPIOL	4.7e-31	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8BD79_BETVU	5.0e-17	67.6	Uncharacterized protein OS=Bet...	[more]
B9TSW7_9LILI	1.7e-9	54.1	Metallothionein-like protein O...	[more]
O49126_FRIAG	1.7e-9	54.1	Metallothionein type I OS=Frit...	[more]
Q3HR41_METSA	3.0e-9	58.3	Metallothionein-like protein O...	[more]

BLAST of Spo21342.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MT3A_ORYSJ	4.5e-11	50.0	Metallothionein-like protein 3...	[more]
MT3A_ORYSI	4.5e-11	50.0	Metallothionein-like protein 3...	[more]
MT3_ACTDE	1.7e-10	48.3	Metallothionein-like protein t...	[more]
MT3_MUSAC	8.5e-10	51.6	Metallothionein-like protein t...	[more]
MT3_MALDO	5.5e-9	49.2	Metallothionein-like protein t...	[more]

BLAST of Spo21342.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G15353.1	4.1e-10	44.1	metallothionein 3	[more]

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo16465	0.69	Barchart \| Table