Spo28098.1 (mRNA) | SpinachBase

Overview


Name	Spo28098.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Mitochondrial import receptor subunit TOM7-1
Location	chr1 : 47043087 .. 47043290 (+)
Sequence length	204

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATGCCGAAAGGAAAGGGAAGAAAAGCTCGAGTTGAAGAAGAAGCTTGCAATACCTTGAAGTGCGTGCAAGATTGGACTAATTGGAGTTTGAAGAAGGCTAAAGTTATCACTCACTATGGTTTAATTCCTCTGATCATCTACATCGGGATGAACTCTGAGCCTAAGCCTACTCTTTCTCAGCTTCTCTCTCCTTTTTAA

mRNA sequence

ATGGCGATGCCGAAAGGAAAGGGAAGAAAAGCTCGAGTTGAAGAAGAAGCTTGCAATACCTTGAAGTGCGTGCAAGATTGGACTAATTGGAGTTTGAAGAAGGCTAAAGTTATCACTCACTATGGTTTAATTCCTCTGATCATCTACATCGGGATGAACTCTGAGCCTAAGCCTACTCTTTCTCAGCTTCTCTCTCCTTTTTAA

Coding sequence (CDS)

ATGGCGATGCCGAAAGGAAAGGGAAGAAAAGCTCGAGTTGAAGAAGAAGCTTGCAATACCTTGAAGTGCGTGCAAGATTGGACTAATTGGAGTTTGAAGAAGGCTAAAGTTATCACTCACTATGGTTTAATTCCTCTGATCATCTACATCGGGATGAACTCTGAGCCTAAGCCTACTCTTTCTCAGCTTCTCTCTCCTTTTTAA

Protein sequence

MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQLLSPF

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo28098	Spo28098	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo28098.1	Spo28098.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo28098.1.exon.1	Spo28098.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo28098.1.CDS.1	Spo28098.1.CDS.1	CDS

Homology

[Close]

BLAST of Spo28098.1 vs. NCBI nr
Match: gi|902225733|gb|KNA20165.1| (hypothetical protein SOVF_054820 [Spinacia oleracea])

HSP 1 Score: 143.7 bits (361), Expect = 1.200e-31
Identity = 67/67 (100.00%), Postives = 67/67 (100.00%), Query Frame = 1


		  
Query: 1  MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL 60
          MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL
Sbjct: 1  MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL 60

Query: 61 SQLLSPF 68
          SQLLSPF
Sbjct: 61 SQLLSPF 67

[Close]

BLAST of Spo28098.1 vs. NCBI nr
Match: gi|731368403|ref|XP_010696128.1| (PREDICTED: mitochondrial import receptor subunit TOM7-1-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 122.9 bits (307), Expect = 2.200e-25
Identity = 60/67 (89.55%), Postives = 63/67 (94.03%), Query Frame = 1


		  
Query: 1  MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL 60
          MAM KGKGRKAR EEEA  TLKCV+DWTNWSLKKA+VITHYGLIPLIIYIGMNSEPKPT+
Sbjct: 1  MAM-KGKGRKARDEEEAGKTLKCVKDWTNWSLKKARVITHYGLIPLIIYIGMNSEPKPTI 60

Query: 61 SQLLSPF 68
          SQLLSPF
Sbjct: 61 SQLLSPF 66

[Close]

BLAST of Spo28098.1 vs. NCBI nr
Match: gi|720023747|ref|XP_010263429.1| (PREDICTED: mitochondrial import receptor subunit TOM7-1-like [Nelumbo nucifera])

HSP 1 Score: 90.1 bits (222), Expect = 1.600e-15
Identity = 41/63 (65.08%), Postives = 51/63 (80.95%), Query Frame = 1


		  
Query: 5  KGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQLL 64
          K  GR    EE +  T+KC++DW+ W++KKAKVITHYG IPL+I IG+NSEPKPTLSQLL
Sbjct: 29 KVSGRSEASEERS--TVKCLKDWSTWTMKKAKVITHYGFIPLVIIIGVNSEPKPTLSQLL 88

Query: 65 SPF 68
          +PF
Sbjct: 89 TPF 89

[Close]

BLAST of Spo28098.1 vs. NCBI nr
Match: gi|769824907|ref|XP_011623506.1| (PREDICTED: mitochondrial import receptor subunit TOM7-1-like [Amborella trichopoda])

HSP 1 Score: 89.7 bits (221), Expect = 2.000e-15
Identity = 40/64 (62.50%), Postives = 52/64 (81.25%), Query Frame = 1


		  
Query: 5  KGKGRKARVE-EEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQL 64
          KGKG+  +++  E  + +KCV++W+ W+LKKAKV+ HYG IPLII IGMNSEPKP LSQL
Sbjct: 8  KGKGKLGKIKGSEPPSKMKCVKEWSTWTLKKAKVVAHYGFIPLIIVIGMNSEPKPYLSQL 67

Query: 65 LSPF 68
          +SPF
Sbjct: 68 VSPF 71

[Close]

BLAST of Spo28098.1 vs. NCBI nr
Match: gi|225443425|ref|XP_002269243.1| (PREDICTED: mitochondrial import receptor subunit TOM7-2 [Vitis vinifera])

HSP 1 Score: 89.4 bits (220), Expect = 2.700e-15
Identity = 41/65 (63.08%), Postives = 51/65 (78.46%), Query Frame = 1


		  
Query: 5  KGKGRKARVEE---EACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLS 64
          KGKG+  +  +   E  +T KC++DW+NW+LKKAKVITHYG IP++I IGMNSEPKP L 
Sbjct: 8  KGKGKSIKGSKDGSEGHSTAKCLKDWSNWALKKAKVITHYGFIPMVIIIGMNSEPKPQLY 67

Query: 65 QLLSP 67
          QLLSP
Sbjct: 68 QLLSP 72

[Close]

BLAST of Spo28098.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RKZ7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_054820 PE=4 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 8.300e-32
Identity = 67/67 (100.00%), Postives = 67/67 (100.00%), Query Frame = 1


		  
Query: 1  MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL 60
          MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL
Sbjct: 1  MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL 60

Query: 61 SQLLSPF 68
          SQLLSPF
Sbjct: 61 SQLLSPF 67

[Close]

BLAST of Spo28098.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BAX4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g177880 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.500e-25
Identity = 60/67 (89.55%), Postives = 63/67 (94.03%), Query Frame = 1


		  
Query: 1  MAMPKGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL 60
          MAM KGKGRKAR EEEA  TLKCV+DWTNWSLKKA+VITHYGLIPLIIYIGMNSEPKPT+
Sbjct: 1  MAM-KGKGRKARDEEEAGKTLKCVKDWTNWSLKKARVITHYGLIPLIIYIGMNSEPKPTI 60

Query: 61 SQLLSPF 68
          SQLLSPF
Sbjct: 61 SQLLSPF 66

[Close]

BLAST of Spo28098.1 vs. UniProtKB/TrEMBL
Match: Q0WLA8_ARATH (TOM7-like protein OS=Arabidopsis thaliana GN=At5g41685 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 9.200e-15
Identity = 38/62 (61.29%), Postives = 47/62 (75.81%), Query Frame = 1


		  
Query: 5  KGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQLL 64
          KGKG K     +  +    V++WTNWSLKKAKV+THYG IPL+I++GMNS+PKP L QLL
Sbjct: 13 KGKGSKGASSSDDKSKFDVVKEWTNWSLKKAKVVTHYGFIPLVIFVGMNSDPKPHLFQLL 72

Query: 65 SP 67
          SP
Sbjct: 73 SP 74

[Close]

BLAST of Spo28098.1 vs. UniProtKB/TrEMBL
Match: S8C8K7_9LAMI (Uncharacterized protein OS=Genlisea aurea GN=M569_11578 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.200e-14
Identity = 44/66 (66.67%), Postives = 48/66 (72.73%), Query Frame = 1


		  
Query: 3  MPKGKGRKARVEEE--ACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTL 62
          M K K   A  EEE  A    K V+ W+NW LKKAKVITHYG IPLII IGMNSEPKP++
Sbjct: 15 MSKKKAAAAEGEEEGSAAAATKLVKQWSNWGLKKAKVITHYGFIPLIIIIGMNSEPKPSI 74

Query: 63 SQLLSP 67
          SQLLSP
Sbjct: 75 SQLLSP 80

[Close]

BLAST of Spo28098.1 vs. UniProtKB/TrEMBL
Match: M0TBX8_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.200e-14
Identity = 41/61 (67.21%), Postives = 46/61 (75.41%), Query Frame = 1


		  
Query: 6  GKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQLLS 65
          GKG K   E  AC   KC ++W+ W++KKAKVITHYG IPLII IGMNSEPKP L QLLS
Sbjct: 14 GKGSKGPEERSAC---KCFKEWSTWAMKKAKVITHYGFIPLIITIGMNSEPKPQLYQLLS 71

Query: 66 P 67
          P
Sbjct: 74 P 71

[Close]

BLAST of Spo28098.1 vs. ExPASy Swiss-Prot
Match: TOM71_ARATH (Mitochondrial import receptor subunit TOM7-1 OS=Arabidopsis thaliana GN=TOM7-1 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 8.300e-17
Identity = 38/62 (61.29%), Postives = 47/62 (75.81%), Query Frame = 1


		  
Query: 5  KGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQLL 64
          KGKG K     +  +    V++WTNWSLKKAKV+THYG IPL+I++GMNS+PKP L QLL
Sbjct: 13 KGKGSKGASSSDDKSKFDVVKEWTNWSLKKAKVVTHYGFIPLVIFVGMNSDPKPHLFQLL 72

Query: 65 SP 67
          SP
Sbjct: 73 SP 74

[Close]

BLAST of Spo28098.1 vs. ExPASy Swiss-Prot
Match: TOM7A_SOLTU (Mitochondrial import receptor subunit TOM7-1 OS=Solanum tuberosum GN=TOM7-1 PE=3 SV=3)

HSP 1 Score: 81.6 bits (200), Expect = 3.500e-15
Identity = 42/71 (59.15%), Postives = 50/71 (70.42%), Query Frame = 1


		  
Query: 1  MAMPKGKG-RKARVEEEACNTL----KCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSE 60
          M  PKGK  +KA   +E    +    K V++W  W+ KKAKVITHYG IPL+I IGMNSE
Sbjct: 1  MLKPKGKNTKKAAAADEDDGAVAVVGKFVKEWGTWTAKKAKVITHYGFIPLVIIIGMNSE 60

Query: 61 PKPTLSQLLSP 67
          PKP+LSQLLSP
Sbjct: 61 PKPSLSQLLSP 71

[Close]

BLAST of Spo28098.1 vs. ExPASy Swiss-Prot
Match: TOM72_ARATH (Mitochondrial import receptor subunit TOM7-2 OS=Arabidopsis thaliana GN=TOM7-2 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.300e-14
Identity = 37/60 (61.67%), Postives = 42/60 (70.00%), Query Frame = 1


		  
Query: 7  KGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQLLSP 66
          KG  +     A +  K  +DWTNWSL+KAKV THYG IPLII IGMNS+PKP L  LLSP
Sbjct: 17 KGSSSSSSSSASSKYKVFKDWTNWSLQKAKVATHYGFIPLIIIIGMNSDPKPHLFHLLSP 76

[Close]

BLAST of Spo28098.1 vs. TAIR (Arabidopsis)
Match: AT5G41685.1 (Mitochondrial outer membrane translocase complex, subunit Tom7)

HSP 1 Score: 87.0 bits (214), Expect = 4.700e-18
Identity = 38/62 (61.29%), Postives = 47/62 (75.81%), Query Frame = 1


		  
Query: 5  KGKGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQLL 64
          KGKG K     +  +    V++WTNWSLKKAKV+THYG IPL+I++GMNS+PKP L QLL
Sbjct: 13 KGKGSKGASSSDDKSKFDVVKEWTNWSLKKAKVVTHYGFIPLVIFVGMNSDPKPHLFQLL 72

Query: 65 SP 67
          SP
Sbjct: 73 SP 74

[Close]

BLAST of Spo28098.1 vs. TAIR (Arabidopsis)
Match: AT1G64220.1 (translocase of outer membrane 7 kDa subunit 2)

HSP 1 Score: 79.7 bits (195), Expect = 7.500e-16
Identity = 37/60 (61.67%), Postives = 42/60 (70.00%), Query Frame = 1


		  
Query: 7  KGRKARVEEEACNTLKCVQDWTNWSLKKAKVITHYGLIPLIIYIGMNSEPKPTLSQLLSP 66
          KG  +     A +  K  +DWTNWSL+KAKV THYG IPLII IGMNS+PKP L  LLSP
Sbjct: 17 KGSSSSSSSSASSKYKVFKDWTNWSLQKAKVATHYGFIPLIIIIGMNSDPKPHLFHLLSP 76

The following BLAST results are available for this feature:

BLAST of Spo28098.1 vs. NCBI nr
BLAST of Spo28098.1 vs. UniProtKB/TrEMBL
BLAST of Spo28098.1 vs. ExPASy Swiss-Prot
BLAST of Spo28098.1 vs. TAIR (Arabidopsis)

BLAST of Spo28098.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902225733\|gb\|KNA20165.1\|	1.2e-31	100.	hypothetical protein SOVF_0548...	[more]
gi\|731368403\|ref\|XP_010696128.1\|	2.2e-25	89.5	PREDICTED: mitochondrial impor...	[more]
gi\|720023747\|ref\|XP_010263429.1\|	1.6e-15	65.0	PREDICTED: mitochondrial impor...	[more]
gi\|769824907\|ref\|XP_011623506.1\|	2.0e-15	62.5	PREDICTED: mitochondrial impor...	[more]
gi\|225443425\|ref\|XP_002269243.1\|	2.7e-15	63.0	PREDICTED: mitochondrial impor...	[more]

BLAST of Spo28098.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RKZ7_SPIOL	8.3e-32	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8BAX4_BETVU	1.5e-25	89.5	Uncharacterized protein OS=Bet...	[more]
Q0WLA8_ARATH	9.2e-15	61.2	TOM7-like protein OS=Arabidops...	[more]
S8C8K7_9LAMI	1.2e-14	66.6	Uncharacterized protein OS=Gen...	[more]
M0TBX8_MUSAM	1.2e-14	67.2	Uncharacterized protein OS=Mus...	[more]

BLAST of Spo28098.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 3


Match Name	E-value	Identity	Description
TOM71_ARATH	8.3e-17	61.2	Mitochondrial import receptor ...	[more]
TOM7A_SOLTU	3.5e-15	59.1	Mitochondrial import receptor ...	[more]
TOM72_ARATH	1.3e-14	61.6	Mitochondrial import receptor ...	[more]

BLAST of Spo28098.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G41685.1	4.7e-18	61.2	Mitochondrial outer membrane t...	[more]
AT1G64220.1	7.5e-16	61.6	translocase of outer membrane ...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012621	Mitochondrial import receptor subunit TOM7	PFAM	PF08038	Tom7	coord: 26..64 score: 4.6
None	No IPR available	PANTHER	PTHR34944	FAMILY NOT NAMED	coord: 1..67 score: 1.6
None	No IPR available	PANTHER	PTHR34944:SF2	MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM7-1-RELATED	coord: 1..67 score: 1.6

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030150	protein import into mitochondrial matrix
cellular_component	GO:0005742	mitochondrial outer membrane translocase complex