Spo27248.1 (mRNA) | SpinachBase

Overview


Name	Spo27248.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Defensin protein
Location	chr5 : 8774905 .. 8775665 (-)
Sequence length	246

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAATGAGGTCGATTGCTGTGGTTTTCCTTGTGTGCCTACTTGTCTTGTCAACAGGTTACTAATGCTATCCTTACTTCCTTACCGTCTTTCAAATTTTTATTTTGGAAACTTTCTTATATAACCCCATATTTTATTTTATTTTGATGTATGATTAAGAGCAATAAATAGATAAAGTTTGCTAATGCTCTGTCCATGACCATACTATACTAATGTTGTTCTTTTTAAACGAGACCATACGTACTTCTAGACATTAATTTCCTTAAATTGGAATCGTTTATGCTTTGATTTTAGACATATATCCCTTTAATTTTACAACCTAACTTTGATCTAATAAGTATGTAATATCGTACATGCATGTTACTATTAAGTATTGATTACTTTTGAGTAGGTCTTCTATGAGACCGCCATATGCATAAGACTGTTTATGTCAGCTTTAAAGTGCACATTGTTAGTTATAAGTAGATTACACCGTATAATGTTGGCTTAATCATGTTTTGTTAGTTTTAATTAGCTAAAACTCCGGCAATTAAATTAACAAAGTTGTTCCTAAATATGTGATTTGTTTGCAGAAGAAATGGGTCCAAGAAAGGCAGACGCTGGATTTTTCAGCTCGAAGAAATGCAAAACACCAAGTAAAACATTCAGGGGACCTTGTGTAAGGAACGCCAACTGTGACACTTCTTGTAGGTATGAGGGATATCCAGCTGGAGATTGCAAGGGTATTCGTAGAAGATGTATTTGTTGTACACATGCTTAA

mRNA sequence

ATGTCAATGAGGTCGATTGCTGTGGTTTTCCTTGTGTGCCTACTTGTCTTGTCAACAGAAGAAATGGGTCCAAGAAAGGCAGACGCTGGATTTTTCAGCTCGAAGAAATGCAAAACACCAAGTAAAACATTCAGGGGACCTTGTGTAAGGAACGCCAACTGTGACACTTCTTGTAGGTATGAGGGATATCCAGCTGGAGATTGCAAGGGTATTCGTAGAAGATGTATTTGTTGTACACATGCTTAA

Coding sequence (CDS)

ATGTCAATGAGGTCGATTGCTGTGGTTTTCCTTGTGTGCCTACTTGTCTTGTCAACAGAAGAAATGGGTCCAAGAAAGGCAGACGCTGGATTTTTCAGCTCGAAGAAATGCAAAACACCAAGTAAAACATTCAGGGGACCTTGTGTAAGGAACGCCAACTGTGACACTTCTTGTAGGTATGAGGGATATCCAGCTGGAGATTGCAAGGGTATTCGTAGAAGATGTATTTGTTGTACACATGCTTAA

Protein sequence

MSMRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTHA

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo27248	Spo27248	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo27248.1	Spo27248.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo27248.1.CDS.2	Spo27248.1.CDS.2	CDS
Spo27248.1.CDS.1	Spo27248.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo27248.1.exon.2	Spo27248.1.exon.2	exon
Spo27248.1.exon.1	Spo27248.1.exon.1	exon

Homology

[Close]

BLAST of Spo27248.1 vs. NCBI nr
Match: gi|902193419|gb|KNA12297.1| (hypothetical protein SOVF_127180 [Spinacia oleracea])

HSP 1 Score: 138.3 bits (347), Expect = 6.100e-30
Identity = 60/60 (100.00%), Postives = 60/60 (100.00%), Query Frame = 1


		  
Query: 22 MGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTHA 81
          MGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTHA
Sbjct: 1  MGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTHA 60

[Close]

BLAST of Spo27248.1 vs. NCBI nr
Match: gi|902180335|gb|KNA09536.1| (hypothetical protein SOVF_152730 [Spinacia oleracea])

HSP 1 Score: 121.3 bits (303), Expect = 7.700e-25
Identity = 50/59 (84.75%), Postives = 56/59 (94.92%), Query Frame = 1


		  
Query: 22 MGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTH 81
          MGPRKA+AG FSS+KCKTPSKTF+G C R++NCDTSCRYEGYPAGDCKGIRRRC+CCTH
Sbjct: 1  MGPRKAEAGIFSSRKCKTPSKTFKGICTRDSNCDTSCRYEGYPAGDCKGIRRRCLCCTH 59

[Close]

BLAST of Spo27248.1 vs. NCBI nr
Match: gi|26393023|sp|P81571.1|DEFD2_SPIOL (RecName: Full=Defensin D2; AltName: Full=Antimicrobial peptide D2; AltName: Full=So-D2)

HSP 1 Score: 97.4 bits (241), Expect = 1.200e-17
Identity = 40/48 (83.33%), Postives = 45/48 (93.75%), Query Frame = 1


		  
Query: 30 GFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCIC 78
          G FSS+KCKTPSKTF+G C R++NCDTSCRYEGYPAGDCKGIRRRC+C
Sbjct: 1  GIFSSRKCKTPSKTFKGICTRDSNCDTSCRYEGYPAGDCKGIRRRCMC 48

[Close]

BLAST of Spo27248.1 vs. NCBI nr
Match: gi|870858983|gb|KMT10447.1| (hypothetical protein BVRB_5g115740 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 84.0 bits (206), Expect = 1.400e-13
Identity = 42/77 (54.55%), Postives = 54/77 (70.13%), Query Frame = 1


		  
Query: 1  MSMRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRY 60
          +SM+  A +FLV LLVL+TE +GPR A+A     + C TPS+ FRG CVR  NC++ C  
Sbjct: 3  LSMKPFAAIFLVLLLVLATE-IGPRVAEA-----RTCGTPSQRFRGLCVRKRNCESVCNS 62

Query: 61 EGYPAGDCKGIRRRCIC 78
          EG+P G C+G RRRCIC
Sbjct: 63 EGFPDGSCQGARRRCIC 73

[Close]

BLAST of Spo27248.1 vs. NCBI nr
Match: gi|870854058|gb|KMT05877.1| (hypothetical protein BVRB_7g165350 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 80.5 bits (197), Expect = 1.500e-12
Identity = 40/76 (52.63%), Postives = 52/76 (68.42%), Query Frame = 1


		  
Query: 2  SMRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYE 61
          SM+  AV+ L+ LL  +TE+ GPR A+A F   KK   PS+T++GPC R+ANC + C  E
Sbjct: 4  SMKPFAVILLMLLLAFATED-GPRVAEALFGRCKK--VPSRTYKGPCARDANCRSVCISE 63

Query: 62 GYPAGDCKGIRRRCIC 78
          GY  GDC G RRRC+C
Sbjct: 64 GYNGGDCHGFRRRCMC 76

[Close]

BLAST of Spo27248.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QYF8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_127180 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 4.200e-30
Identity = 60/60 (100.00%), Postives = 60/60 (100.00%), Query Frame = 1


		  
Query: 22 MGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTHA 81
          MGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTHA
Sbjct: 1  MGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTHA 60

[Close]

BLAST of Spo27248.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QSM3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_152730 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 5.300e-25
Identity = 50/59 (84.75%), Postives = 56/59 (94.92%), Query Frame = 1


		  
Query: 22 MGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCICCTH 81
          MGPRKA+AG FSS+KCKTPSKTF+G C R++NCDTSCRYEGYPAGDCKGIRRRC+CCTH
Sbjct: 1  MGPRKAEAGIFSSRKCKTPSKTFKGICTRDSNCDTSCRYEGYPAGDCKGIRRRCLCCTH 59

[Close]

BLAST of Spo27248.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CA30_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g115740 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 9.500e-14
Identity = 42/77 (54.55%), Postives = 54/77 (70.13%), Query Frame = 1


		  
Query: 1  MSMRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRY 60
          +SM+  A +FLV LLVL+TE +GPR A+A     + C TPS+ FRG CVR  NC++ C  
Sbjct: 3  LSMKPFAAIFLVLLLVLATE-IGPRVAEA-----RTCGTPSQRFRGLCVRKRNCESVCNS 62

Query: 61 EGYPAGDCKGIRRRCIC 78
          EG+P G C+G RRRCIC
Sbjct: 63 EGFPDGSCQGARRRCIC 73

[Close]

BLAST of Spo27248.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BXF9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g165350 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.000e-12
Identity = 40/76 (52.63%), Postives = 52/76 (68.42%), Query Frame = 1


		  
Query: 2  SMRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYE 61
          SM+  AV+ L+ LL  +TE+ GPR A+A F   KK   PS+T++GPC R+ANC + C  E
Sbjct: 4  SMKPFAVILLMLLLAFATED-GPRVAEALFGRCKK--VPSRTYKGPCARDANCRSVCISE 63

Query: 62 GYPAGDCKGIRRRCIC 78
          GY  GDC G RRRC+C
Sbjct: 64 GYNGGDCHGFRRRCMC 76

[Close]

BLAST of Spo27248.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CEL9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g115750 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.800e-12
Identity = 40/75 (53.33%), Postives = 50/75 (66.67%), Query Frame = 1


		  
Query: 3  MRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEG 62
          MR  A + L  LLVL+TE +GPR A+A     + C TPS  FRG CV   NC+++C  EG
Sbjct: 5  MRPFAAILLALLLVLATE-IGPRVAEA-----RTCVTPSHRFRGICVSKRNCESACNSEG 64

Query: 63 YPAGDCKGIRRRCIC 78
          +P G+CKG RRRCIC
Sbjct: 65 FPDGNCKGFRRRCIC 73

[Close]

BLAST of Spo27248.1 vs. ExPASy Swiss-Prot
Match: DEFD2_SPIOL (Defensin D2 (Fragment) OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 7.400e-20
Identity = 40/48 (83.33%), Postives = 45/48 (93.75%), Query Frame = 1


		  
Query: 30 GFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCIC 78
          G FSS+KCKTPSKTF+G C R++NCDTSCRYEGYPAGDCKGIRRRC+C
Sbjct: 1  GIFSSRKCKTPSKTFKGICTRDSNCDTSCRYEGYPAGDCKGIRRRCMC 48

[Close]

BLAST of Spo27248.1 vs. ExPASy Swiss-Prot
Match: DEFD7_SPIOL (Defensin D7 (Fragment) OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.800e-13
Identity = 31/38 (81.58%), Postives = 35/38 (92.11%), Query Frame = 1


		  
Query: 30 GFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGD 68
          G FSS+KCKTPSKTF+G C R++NCDTSCRYEGYPAGD
Sbjct: 1  GIFSSRKCKTPSKTFKGYCTRDSNCDTSCRYEGYPAGD 38

[Close]

BLAST of Spo27248.1 vs. ExPASy Swiss-Prot
Match: DEF_NELNU (Defensin-like protein OS=Nelumbo nucifera PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 3.300e-12
Identity = 36/75 (48.00%), Postives = 50/75 (66.67%), Query Frame = 1


		  
Query: 3  MRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYEG 62
          MR  + + LV LLV +TE MGP+ A+A     + C++ S  F+G C+ + NC + C+ EG
Sbjct: 5  MRLFSSLVLVLLLVTATE-MGPKVAEA-----RTCESQSHRFKGACLSDTNCASVCQTEG 64

Query: 63 YPAGDCKGIRRRCIC 78
          +PAGDCKG RRRC C
Sbjct: 65 FPAGDCKGARRRCFC 73

[Close]

BLAST of Spo27248.1 vs. ExPASy Swiss-Prot
Match: DEF04_ARATH (Defensin-like protein 4 OS=Arabidopsis thaliana GN=PDF2.1 PE=2 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 2.400e-10
Identity = 31/76 (40.79%), Postives = 46/76 (60.53%), Query Frame = 1


		  
Query: 2  SMRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYE 61
          SMR I+ V  + ++ ++T  MGP   +A     + C + S+ F+G CV + NC+  C  E
Sbjct: 4  SMRLISAVLFLVMIFVATG-MGPVTVEA-----RTCASQSQRFKGKCVSDTNCENVCHNE 63

Query: 62 GYPAGDCKGIRRRCIC 78
          G+P GDC+G RRRC C
Sbjct: 64 GFPGGDCRGFRRRCFC 73

[Close]

BLAST of Spo27248.1 vs. ExPASy Swiss-Prot
Match: DEF_PETIN (Defensin-like protein OS=Petunia integrifolia PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 7.000e-10
Identity = 32/76 (42.11%), Postives = 46/76 (60.53%), Query Frame = 1


		  
Query: 2  SMRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYE 61
          S+R  A  FL+ +L LSTE MGP  +      ++ C++ S  F G CVR +NC + C+ E
Sbjct: 4  SIRLFATFFLIAMLFLSTE-MGPMTSA----EARTCESQSHRFHGTCVRESNCASVCQTE 63

Query: 62 GYPAGDCKGIRRRCIC 78
          G+  G+C+  RRRC C
Sbjct: 64 GFIGGNCRAFRRRCFC 74

[Close]

BLAST of Spo27248.1 vs. TAIR (Arabidopsis)
Match: AT2G02120.1 (Scorpion toxin-like knottin superfamily protein)

HSP 1 Score: 65.9 bits (159), Expect = 1.300e-11
Identity = 31/76 (40.79%), Postives = 46/76 (60.53%), Query Frame = 1


		  
Query: 2  SMRSIAVVFLVCLLVLSTEEMGPRKADAGFFSSKKCKTPSKTFRGPCVRNANCDTSCRYE 61
          SMR I+ V  + ++ ++T  MGP   +A     + C + S+ F+G CV + NC+  C  E
Sbjct: 4  SMRLISAVLFLVMIFVATG-MGPVTVEA-----RTCASQSQRFKGKCVSDTNCENVCHNE 63

Query: 62 GYPAGDCKGIRRRCIC 78
          G+P GDC+G RRRC C
Sbjct: 64 GFPGGDCRGFRRRCFC 73

[Close]

BLAST of Spo27248.1 vs. TAIR (Arabidopsis)
Match: AT1G61070.1 (low-molecular-weight cysteine-rich 66)

HSP 1 Score: 58.2 bits (139), Expect = 2.800e-9
Identity = 21/44 (47.73%), Postives = 30/44 (68.18%), Query Frame = 1


		  
Query: 34 SKKCKTPSKTFRGPCVRNANCDTSCRYEGYPAGDCKGIRRRCIC 78
          ++ C+T S  F GPC+ ++NC   C  EG+  GDC+G RRRC+C
Sbjct: 29 ARTCETSSNLFNGPCLSSSNCANVCHNEGFSDGDCRGFRRRCLC 72

The following BLAST results are available for this feature:

BLAST of Spo27248.1 vs. NCBI nr
BLAST of Spo27248.1 vs. UniProtKB/TrEMBL
BLAST of Spo27248.1 vs. ExPASy Swiss-Prot
BLAST of Spo27248.1 vs. TAIR (Arabidopsis)

BLAST of Spo27248.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902193419\|gb\|KNA12297.1\|	6.1e-30	100.	hypothetical protein SOVF_1271...	[more]
gi\|902180335\|gb\|KNA09536.1\|	7.7e-25	84.7	hypothetical protein SOVF_1527...	[more]
gi\|26393023\|sp\|P81571.1\|DEFD2_SPIOL	1.2e-17	83.3	RecName: Full=Defensin D2; Alt...	[more]
gi\|870858983\|gb\|KMT10447.1\|	1.4e-13	54.5	hypothetical protein BVRB_5g11...	[more]
gi\|870854058\|gb\|KMT05877.1\|	1.5e-12	52.6	hypothetical protein BVRB_7g16...	[more]

BLAST of Spo27248.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QYF8_SPIOL	4.2e-30	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QSM3_SPIOL	5.3e-25	84.7	Uncharacterized protein OS=Spi...	[more]
A0A0J8CA30_BETVU	9.5e-14	54.5	Uncharacterized protein OS=Bet...	[more]
A0A0J8BXF9_BETVU	1.0e-12	52.6	Uncharacterized protein OS=Bet...	[more]
A0A0J8CEL9_BETVU	1.8e-12	53.3	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo27248.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
DEFD2_SPIOL	7.4e-20	83.3	Defensin D2 (Fragment) OS=Spin...	[more]
DEFD7_SPIOL	1.8e-13	81.5	Defensin D7 (Fragment) OS=Spin...	[more]
DEF_NELNU	3.3e-12	48.0	Defensin-like protein OS=Nelum...	[more]
DEF04_ARATH	2.4e-10	40.7	Defensin-like protein 4 OS=Ara...	[more]
DEF_PETIN	7.0e-10	42.1	Defensin-like protein OS=Petun...	[more]

BLAST of Spo27248.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G02120.1	1.3e-11	40.7	Scorpion toxin-like knottin su...	[more]
AT1G61070.1	2.8e-9	47.7	low-molecular-weight cysteine-...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003614	Knottin, scorpion toxin-like	GENE3D	3.30.30.10		coord: 35..80 score: 2.7
IPR003614	Knottin, scorpion toxin-like	SMART	SM00505	gth_4	coord: 36..78 score: 0.
IPR003614	Knottin, scorpion toxin-like	unknown	SSF57095	Scorpion toxin-like	coord: 35..79 score: 4.38
IPR008176	Defensin, plant	PRINTS	PR00288	PUROTHIONIN	coord: 66..80 score: 1.6E-5coord: 36..51 score: 1.
IPR008176	Defensin, plant	PROSITE	PS00940	GAMMA_THIONIN	coord: 35..58 scor
None	No IPR available	PANTHER	PTHR33147	FAMILY NOT NAMED	coord: 1..77 score: 7.4
None	No IPR available	PFAM	PF00304	Gamma-thionin	coord: 36..78 score: 1.6

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0042742	defense response to bacterium
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0031640	killing of cells of other organism
cellular_component	GO:0005618	cell wall
cellular_component	GO:0005576	extracellular region