Spo25251.1 (mRNA) | SpinachBase

Overview


Name	Spo25251.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase
Location	chr5 : 7554654 .. 7556291 (-)
Sequence length	642

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGATTTAAATTACTAATTATTGTGGCTCAGTGGAAAGTAACGCTTCTATATGTAGGTAATTAATTATTAGTAGCTCATTGCATTAAAGTAAGAATCAAACAAAGAGAGAAATTAAAGAGAGGACTACGATGGTATTTGAGGTGAAGCAGGTGCTTCGTATGAAGGAAGGAACCGATGAAACCAGTTACGTAAAAACATCCTACATTCAGGTACGTGGTTTTATAGAAAAACGATCCCAAAACAAATTATTAGTGATAGACGTTTGTTCTAAAGTAGTCACAAACGACAATGCGATAAATAACGTATGTGCGATTCGATCTTAGAAGTATAAAGTGTTTGATAAATTAATTGTTAATTATTTATTGTGTAAAAATAATCATTTAAGACATACTCCGTAGACGTAAAATAATGTAATTAAGTTTACAACGGTTATAATTTTAGACAATATTAGGTTGGTTGCCATGCAAGCCGGCCACGTTTAACAATTATATGAAACATGCGTATTTGATTTTTTTTTTTCCAATAATTATTAAATGTAATCATAATCTCGGGTAACGTAGCCAACTTTTCTGTGTCATCTTTAAAAGGTCTTTATTAGACATTTGATTTTTTTTTTAATGACGCATTAGGAGATAACGTACACGAGTAATTTTCAGTGCATATTTTAGTACTTATATATATTTTTTGAAGGTTAATACTATATAAATTGTTATGGTTAATTTATGTATTTATAGAAAAATATTGCTGTTGTTGGTAAAATTTGACTATTTATTTTTTCAATTTATTTTTATTTTAAAGACATAATCAAACATCAATGCCATTGTAGATCTACGCATTTTGCAAAAAGTAACAAGTACCAAGAGAGGAATAAAGGGAGAAAATGAGTTACCTAACATTTATTCTCTTCGTTTTTGTTTCAATCTTCATATGTAATAGAGCGGAAAATGTTTGGTTGACCCTAAGGGTTAGCAACTTTATATCTTAAGGGTTAGCAACTTTCAATCTTCAATCTTTATATGTATCTTAAGGGTTAGCAACCCTTAAGATACATATAACATAAAATAATATATAAGTGTAATTAATTGGAGAGAGAAAGTGTTGTAAGGTGAGTTTCAATGCATTTGTAACCAATCATAACTTAATATTTAAAGGGTTACTAACCCTTAGGGTTACTCTATCATTGTCCTTTGATTTTCATGCAACAGAGGCAGGTGCTATCATTGACAAAATCCCTAAAAGAAAAAGCCATAATGGAAATATATTGCAAAGAACAACCAACAACCTTATGCATAGCCGACTTGGGTTGTTCCTCTGCTGAGCCTAACACCTTAGGGGTGGTCTCTGAACTCATAGATGCAATCGAAAAGGCTCGTCGAGAGATCGGAAATGGGCACCAAGAGTATCAAGTTTATTTAAATGATCTTCCATGGAATGATTTCAACACTATCTTTAGGTCATTGGGAAGCTTTGAAGATAACTTGAAGAAACAAGTTGGGAATAATTTTGGACATTGTTTTGTGAATGGTGTCCCTGGTTCATTCTATGGCAGGCTATTCCCCACAAAACATTTGCATTTTGTCCATTCTTCTTATAGCCTTATGTGGCTTTCTCAGGCAGTGGCGTATCCAGGATTTTGA

mRNA sequence

TCGATTTAAATTACTAATTATTGTGGCTCAGTGGAAAGTAACGCTTCTATATGTAGGTAATTAATTATTAGTAGCTCATTGCATTAAAGTAAGAATCAAACAAAGAGAGAAATTAAAGAGAGGACTACGATGGTATTTGAGGTGAAGCAGGTGCTTCGTATGAAGGAAGGAACCGATGAAACCAGTTACGTAAAAACATCCTACATTCAGAGGCAGGTGCTATCATTGACAAAATCCCTAAAAGAAAAAGCCATAATGGAAATATATTGCAAAGAACAACCAACAACCTTATGCATAGCCGACTTGGGTTGTTCCTCTGCTGAGCCTAACACCTTAGGGGTGGTCTCTGAACTCATAGATGCAATCGAAAAGGCTCGTCGAGAGATCGGAAATGGGCACCAAGAGTATCAAGTTTATTTAAATGATCTTCCATGGAATGATTTCAACACTATCTTTAGGTCATTGGGAAGCTTTGAAGATAACTTGAAGAAACAAGTTGGGAATAATTTTGGACATTGTTTTGTGAATGGTGTCCCTGGTTCATTCTATGGCAGGCTATTCCCCACAAAACATTTGCATTTTGTCCATTCTTCTTATAGCCTTATGTGGCTTTCTCAGGCAGTGGCGTATCCAGGATTTTGA

Coding sequence (CDS)

ATGGTATTTGAGGTGAAGCAGGTGCTTCGTATGAAGGAAGGAACCGATGAAACCAGTTACGTAAAAACATCCTACATTCAGAGGCAGGTGCTATCATTGACAAAATCCCTAAAAGAAAAAGCCATAATGGAAATATATTGCAAAGAACAACCAACAACCTTATGCATAGCCGACTTGGGTTGTTCCTCTGCTGAGCCTAACACCTTAGGGGTGGTCTCTGAACTCATAGATGCAATCGAAAAGGCTCGTCGAGAGATCGGAAATGGGCACCAAGAGTATCAAGTTTATTTAAATGATCTTCCATGGAATGATTTCAACACTATCTTTAGGTCATTGGGAAGCTTTGAAGATAACTTGAAGAAACAAGTTGGGAATAATTTTGGACATTGTTTTGTGAATGGTGTCCCTGGTTCATTCTATGGCAGGCTATTCCCCACAAAACATTTGCATTTTGTCCATTCTTCTTATAGCCTTATGTGGCTTTCTCAGGCAGTGGCGTATCCAGGATTTTGA

Protein sequence

MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLGCSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKKQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQAVAYPGF

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo25251	Spo25251	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo25251.1	Spo25251.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25251.1.utr5p.1	Spo25251.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25251.1.CDS.2	Spo25251.1.CDS.2	CDS
Spo25251.1.CDS.1	Spo25251.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo25251.1.exon.2	Spo25251.1.exon.2	exon
Spo25251.1.exon.1	Spo25251.1.exon.1	exon

Homology

[Close]

BLAST of Spo25251.1 vs. NCBI nr
Match: gi|902217141|gb|KNA17621.1| (hypothetical protein SOVF_078430 [Spinacia oleracea])

HSP 1 Score: 335.9 bits (860), Expect = 4.200e-89
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 1


		  
Query: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60
           MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG
Sbjct: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60

Query: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120
           CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK
Sbjct: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120

Query: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ
Sbjct: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 163

[Close]

BLAST of Spo25251.1 vs. NCBI nr
Match: gi|731335426|ref|XP_010678722.1| (PREDICTED: salicylate carboxymethyltransferase-like isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 272.3 bits (695), Expect = 5.600e-70
Identity = 130/162 (80.25%), Postives = 145/162 (89.51%), Query Frame = 1


		  
Query: 2   VFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLGC 61
           + +V+QVLRMKEGTDETSYVKTSYIQ+QVL LTKS+KEKAI E+YCKE+P  LCIADLGC
Sbjct: 3   MIQVEQVLRMKEGTDETSYVKTSYIQKQVLLLTKSIKEKAIKELYCKERPEILCIADLGC 62

Query: 62  SSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKK 121
           SSAE NTL V+SELID++E ARRE+GN  QEYQVYLNDLP NDFNTIFRSL  F D LK 
Sbjct: 63  SSAESNTLSVISELIDSVENARRELGNRKQEYQVYLNDLPGNDFNTIFRSLTKFTDQLKL 122

Query: 122 QVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           ++G+ FGHCFVNGVPGSFYGRLFP+KHLHFVHSSYSLMWLSQ
Sbjct: 123 RMGDGFGHCFVNGVPGSFYGRLFPSKHLHFVHSSYSLMWLSQ 164

[Close]

BLAST of Spo25251.1 vs. NCBI nr
Match: gi|731335424|ref|XP_010678721.1| (PREDICTED: salicylate carboxymethyltransferase-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 266.5 bits (680), Expect = 3.100e-68
Identity = 130/166 (78.31%), Postives = 145/166 (87.35%), Query Frame = 1


		  
Query: 2   VFEVKQVLRMKEGTDETSYVKTSYIQ----RQVLSLTKSLKEKAIMEIYCKEQPTTLCIA 61
           + +V+QVLRMKEGTDETSYVKTSYIQ    +QVL LTKS+KEKAI E+YCKE+P  LCIA
Sbjct: 3   MIQVEQVLRMKEGTDETSYVKTSYIQVRTAKQVLLLTKSIKEKAIKELYCKERPEILCIA 62

Query: 62  DLGCSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFED 121
           DLGCSSAE NTL V+SELID++E ARRE+GN  QEYQVYLNDLP NDFNTIFRSL  F D
Sbjct: 63  DLGCSSAESNTLSVISELIDSVENARRELGNRKQEYQVYLNDLPGNDFNTIFRSLTKFTD 122

Query: 122 NLKKQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
            LK ++G+ FGHCFVNGVPGSFYGRLFP+KHLHFVHSSYSLMWLSQ
Sbjct: 123 QLKLRMGDGFGHCFVNGVPGSFYGRLFPSKHLHFVHSSYSLMWLSQ 168

[Close]

BLAST of Spo25251.1 vs. NCBI nr
Match: gi|870863842|gb|KMT14989.1| (hypothetical protein BVRB_3g064940 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 241.9 bits (616), Expect = 8.200e-61
Identity = 113/163 (69.33%), Postives = 136/163 (83.44%), Query Frame = 1


		  
Query: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60
           M   +KQV RMKEGT+ETSY K SY+Q++ LSLTK ++EKAI E Y KEQPTTLCIADLG
Sbjct: 1   MATPLKQVFRMKEGTNETSYAKASYLQKEGLSLTKPIREKAIREFYYKEQPTTLCIADLG 60

Query: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120
           CSS E + L  V ELI+++E ARRE+G G +EYQ+YLNDLP NDFNT+FRSLGSF D LK
Sbjct: 61  CSSVELSNLSPVFELIESVENARRELGKGPKEYQIYLNDLPGNDFNTVFRSLGSFGDKLK 120

Query: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           +++G++FGHCFVNG PGSF+GRLF + HLHF+HSSYSL WLSQ
Sbjct: 121 QEMGDDFGHCFVNGAPGSFHGRLFASNHLHFIHSSYSLHWLSQ 163

[Close]

BLAST of Spo25251.1 vs. NCBI nr
Match: gi|731325038|ref|XP_010673295.1| (PREDICTED: salicylate carboxymethyltransferase-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 241.9 bits (616), Expect = 8.200e-61
Identity = 113/163 (69.33%), Postives = 136/163 (83.44%), Query Frame = 1


		  
Query: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60
           M   +KQV RMKEGT+ETSY K SY+Q++ LSLTK ++EKAI E Y KEQPTTLCIADLG
Sbjct: 1   MATPLKQVFRMKEGTNETSYAKASYLQKEGLSLTKPIREKAIREFYYKEQPTTLCIADLG 60

Query: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120
           CSS E + L  V ELI+++E ARRE+G G +EYQ+YLNDLP NDFNT+FRSLGSF D LK
Sbjct: 61  CSSVELSNLSPVFELIESVENARRELGKGPKEYQIYLNDLPGNDFNTVFRSLGSFGDKLK 120

Query: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           +++G++FGHCFVNG PGSF+GRLF + HLHF+HSSYSL WLSQ
Sbjct: 121 QEMGDDFGHCFVNGAPGSFHGRLFASNHLHFIHSSYSLHWLSQ 163

[Close]

BLAST of Spo25251.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RDN0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_078430 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 2.900e-89
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 1


		  
Query: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60
           MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG
Sbjct: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60

Query: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120
           CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK
Sbjct: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120

Query: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ
Sbjct: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 163

[Close]

BLAST of Spo25251.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CSG5_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g064940 PE=4 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 5.700e-61
Identity = 113/163 (69.33%), Postives = 136/163 (83.44%), Query Frame = 1


		  
Query: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60
           M   +KQV RMKEGT+ETSY K SY+Q++ LSLTK ++EKAI E Y KEQPTTLCIADLG
Sbjct: 1   MATPLKQVFRMKEGTNETSYAKASYLQKEGLSLTKPIREKAIREFYYKEQPTTLCIADLG 60

Query: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120
           CSS E + L  V ELI+++E ARRE+G G +EYQ+YLNDLP NDFNT+FRSLGSF D LK
Sbjct: 61  CSSVELSNLSPVFELIESVENARRELGKGPKEYQIYLNDLPGNDFNTVFRSLGSFGDKLK 120

Query: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           +++G++FGHCFVNG PGSF+GRLF + HLHF+HSSYSL WLSQ
Sbjct: 121 QEMGDDFGHCFVNGAPGSFHGRLFASNHLHFIHSSYSLHWLSQ 163

[Close]

BLAST of Spo25251.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CMM0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g064950 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.400e-59
Identity = 113/163 (69.33%), Postives = 134/163 (82.21%), Query Frame = 1


		  
Query: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60
           M   +KQV RMKEGT+ETSY K SY+Q++VL LTK L+EK I E Y +EQPTTLCIADLG
Sbjct: 1   MATTLKQVFRMKEGTNETSYAKASYLQKEVLLLTKPLREKVIREFYYREQPTTLCIADLG 60

Query: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120
           CSS E + L  V  L++++E ARRE+  G QEYQ+YLNDLP NDFNTIFRSLGSF D LK
Sbjct: 61  CSSVELSNLSPVFGLLESVENARRELEKGPQEYQIYLNDLPGNDFNTIFRSLGSFGDKLK 120

Query: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           +++G++FGH FVNGVPGSFYGRLF +KHLHF+HSSYSL WLSQ
Sbjct: 121 QEMGDDFGHHFVNGVPGSFYGRLFASKHLHFIHSSYSLHWLSQ 163

[Close]

BLAST of Spo25251.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FGK9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g064950 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.400e-59
Identity = 113/163 (69.33%), Postives = 134/163 (82.21%), Query Frame = 1


		  
Query: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60
           M   +KQV RMKEGT+ETSY K SY+Q++VL LTK L+EK I E Y +EQPTTLCIADLG
Sbjct: 1   MATTLKQVFRMKEGTNETSYAKASYLQKEVLLLTKPLREKVIREFYYREQPTTLCIADLG 60

Query: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120
           CSS E + L  V  L++++E ARRE+  G QEYQ+YLNDLP NDFNTIFRSLGSF D LK
Sbjct: 61  CSSVELSNLSPVFGLLESVENARRELEKGPQEYQIYLNDLPGNDFNTIFRSLGSFGDKLK 120

Query: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           +++G++FGH FVNGVPGSFYGRLF +KHLHF+HSSYSL WLSQ
Sbjct: 121 QEMGDDFGHHFVNGVPGSFYGRLFASKHLHFIHSSYSLHWLSQ 163

[Close]

BLAST of Spo25251.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CSP4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g064930 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 5.300e-59
Identity = 112/163 (68.71%), Postives = 134/163 (82.21%), Query Frame = 1


		  
Query: 1   MVFEVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLG 60
           M  ++KQV RM EGTDE SY KTSYIQ++VLSLTK ++EKAI E Y +EQPTTLCIADLG
Sbjct: 3   MSTKLKQVFRMNEGTDEGSYAKTSYIQKEVLSLTKPIREKAIREFYYREQPTTLCIADLG 62

Query: 61  CSSAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLK 120
           CSS E + L  V  L++++E ARRE+G   QEYQ+YLNDLP NDFNTIFRSL SF D LK
Sbjct: 63  CSSVELSNLSPVFGLLESVENARRELGKEPQEYQIYLNDLPGNDFNTIFRSLTSFGDKLK 122

Query: 121 KQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           +++G++FGHCF+NG  GSFYGRLF +KHLHF+HSSYSL WLSQ
Sbjct: 123 QEMGDDFGHCFINGALGSFYGRLFASKHLHFIHSSYSLHWLSQ 165

[Close]

BLAST of Spo25251.1 vs. ExPASy Swiss-Prot
Match: SAMT_CLABR (Salicylate carboxymethyltransferase OS=Clarkia breweri GN=SAMT PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 5.500e-41
Identity = 92/162 (56.79%), Postives = 114/162 (70.37%), Query Frame = 1


		  
Query: 4   EVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTT-LCIADLGCS 63
           +V+QVL MK G  E SY   S+IQRQV+S+TK + E AI  +Y  +  TT L IADLGCS
Sbjct: 2   DVRQVLHMKGGAGENSYAMNSFIQRQVISITKPITEAAITALYSGDTVTTRLAIADLGCS 61

Query: 64  SAEPNTLGVVSELIDAIEKARREIGNGHQ-EYQVYLNDLPWNDFNTIFRSLGSFEDNLKK 123
           S  PN L  V+ELI  +E+ R+++G  +  EYQ++LNDLP NDFN IFRSL   E+++  
Sbjct: 62  SG-PNALFAVTELIKTVEELRKKMGRENSPEYQIFLNDLPGNDFNAIFRSL-PIENDVD- 121

Query: 124 QVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
                 G CF+NGVPGSFYGRLFP   LHF+HSSYSLMWLSQ
Sbjct: 122 ------GVCFINGVPGSFYGRLFPRNTLHFIHSSYSLMWLSQ 154

[Close]

BLAST of Spo25251.1 vs. ExPASy Swiss-Prot
Match: JMT_BRARP (Jasmonate O-methyltransferase OS=Brassica rapa subsp. pekinensis GN=JMT PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 4.000e-31
Identity = 75/170 (44.12%), Postives = 104/170 (61.18%), Query Frame = 1


		  
Query: 4   EVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPT-TLCIADLGCS 63
           EV ++L M +G  ETSY K S +Q  ++SL + + ++A+ ++  +     +  IADLGCS
Sbjct: 2   EVMRILHMNKGNGETSYAKNSIVQSNIISLGRRVMDEALKKLMIRNSEILSFGIADLGCS 61

Query: 64  SAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKKQ 123
           S  PN+L  +S +++ I+    ++     E  + LNDLP NDFN IF SL  F D +KK+
Sbjct: 62  SG-PNSLLSISNIVETIQNLCHDLDRPVPELSLSLNDLPSNDFNYIFASLPEFYDRVKKR 121

Query: 124 VGN---------NFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
             N         + G CFV+ VPGSFYGRLFP + LHFVHSS SL WLSQ
Sbjct: 122 DNNYESLGFEHGSGGPCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLSQ 170

[Close]

BLAST of Spo25251.1 vs. ExPASy Swiss-Prot
Match: JMT_ARATH (Jasmonate O-methyltransferase OS=Arabidopsis thaliana GN=JMT PE=1 SV=3)

HSP 1 Score: 129.8 bits (325), Expect = 2.800e-29
Identity = 76/167 (45.51%), Postives = 101/167 (60.48%), Query Frame = 1


		  
Query: 4   EVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQP-TTLCIADLGCS 63
           EV +VL M +G  ETSY K S  Q  ++SL + + ++A+ ++       +++ IADLGCS
Sbjct: 2   EVMRVLHMNKGNGETSYAKNSTAQSNIISLGRRVMDEALKKLMMSNSEISSIGIADLGCS 61

Query: 64  SAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKK- 123
           S  PN+L  +S ++D I     ++     E +V LNDLP NDFN I  SL  F D +   
Sbjct: 62  SG-PNSLLSISNIVDTIHNLCPDLDRPVPELRVSLNDLPSNDFNYICASLPEFYDRVNNN 121

Query: 124 QVGNNFGH-----CFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           + G  FG      CFV+ VPGSFYGRLFP + LHFVHSS SL WLSQ
Sbjct: 122 KEGLGFGRGGGESCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLSQ 167

[Close]

BLAST of Spo25251.1 vs. ExPASy Swiss-Prot
Match: XMT1_COFAR (7-methylxanthosine synthase 1 OS=Coffea arabica GN=XMT1 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.800e-29
Identity = 69/165 (41.82%), Postives = 101/165 (61.21%), Query Frame = 1


		  
Query: 4   EVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTT---LCIADLG 63
           E+++VLRM  G  +TSY K S   + VL+  K + E+ + E+     P     + +ADLG
Sbjct: 2   ELQEVLRMNGGEGDTSYAKNSAYNQLVLAKVKPVLEQCVRELLRANLPNINKCIKVADLG 61

Query: 64  CSSAEPNTLGVVSELIDAIEKARREIGNGHQE--YQVYLNDLPWNDFNTIFRSLGSFEDN 123
           C+S  PNTL  V +++ +I+K  +E  N  +    Q++LNDL  NDFN++F+ L SF   
Sbjct: 62  CASG-PNTLLTVRDIVQSIDKVGQEKKNELERPTIQIFLNDLFPNDFNSVFKLLPSFYRK 121

Query: 124 LKKQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           L+K+ G   G C +  +PGSFY RLFP + +HF+HS Y L WLSQ
Sbjct: 122 LEKENGRKIGSCLIGAMPGSFYSRLFPEESMHFLHSCYCLQWLSQ 165

[Close]

BLAST of Spo25251.1 vs. ExPASy Swiss-Prot
Match: XMT1_COFCA (7-methylxanthosine synthase 1 OS=Coffea canephora GN=XMT1 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.800e-29
Identity = 69/165 (41.82%), Postives = 101/165 (61.21%), Query Frame = 1


		  
Query: 4   EVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTT---LCIADLG 63
           E+++VLRM  G  +TSY K S   + VL+  K + E+ + E+     P     + +ADLG
Sbjct: 2   ELQEVLRMNGGEGDTSYAKNSAYNQLVLAKVKPVLEQCVRELLRANLPNINKCIKVADLG 61

Query: 64  CSSAEPNTLGVVSELIDAIEKARREIGNGHQE--YQVYLNDLPWNDFNTIFRSLGSFEDN 123
           C+S  PNTL  V +++ +I+K  +E  N  +    Q++LNDL  NDFN++F+ L SF   
Sbjct: 62  CASG-PNTLLTVRDIVQSIDKVGQEKKNELERPTIQIFLNDLFPNDFNSVFKLLPSFYRK 121

Query: 124 LKKQVGNNFGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           L+K+ G   G C +  +PGSFY RLFP + +HF+HS Y L WLSQ
Sbjct: 122 LEKENGRKIGSCLIGAMPGSFYSRLFPEESMHFLHSCYCLQWLSQ 165

[Close]

BLAST of Spo25251.1 vs. TAIR (Arabidopsis)
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase)

HSP 1 Score: 129.8 bits (325), Expect = 1.600e-30
Identity = 76/167 (45.51%), Postives = 101/167 (60.48%), Query Frame = 1


		  
Query: 4   EVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQP-TTLCIADLGCS 63
           EV +VL M +G  ETSY K S  Q  ++SL + + ++A+ ++       +++ IADLGCS
Sbjct: 2   EVMRVLHMNKGNGETSYAKNSTAQSNIISLGRRVMDEALKKLMMSNSEISSIGIADLGCS 61

Query: 64  SAEPNTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKK- 123
           S  PN+L  +S ++D I     ++     E +V LNDLP NDFN I  SL  F D +   
Sbjct: 62  SG-PNSLLSISNIVDTIHNLCPDLDRPVPELRVSLNDLPSNDFNYICASLPEFYDRVNNN 121

Query: 124 QVGNNFGH-----CFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
           + G  FG      CFV+ VPGSFYGRLFP + LHFVHSS SL WLSQ
Sbjct: 122 KEGLGFGRGGGESCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLSQ 167

[Close]

BLAST of Spo25251.1 vs. TAIR (Arabidopsis)
Match: AT3G21950.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 126.3 bits (316), Expect = 1.800e-29
Identity = 72/157 (45.86%), Postives = 96/157 (61.15%), Query Frame = 1


		  
Query: 8   VLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCK-EQPTTLCIADLGCSSAEP 67
           V+ MK G  E SY   S  Q+ + S  K    K++ E+  K + P  + +ADLGCSS E 
Sbjct: 3   VVSMKGGDGEHSYANNSEAQKSITSDAKPEVMKSVNEMIVKMDFPGCIKVADLGCSSGE- 62

Query: 68  NTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKKQVGNN 127
           NT  V+SE+++ I    ++ G    E    LNDLP NDFNT F+ + SF + LK  V   
Sbjct: 63  NTFLVMSEIVNTIITTYQQNGQNLPEIDCCLNDLPENDFNTTFKLIPSFHEKLKMNVK-- 122

Query: 128 FGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
            G+C+V+G PGSFY RLFP+K LHFVHSS+ L WLS+
Sbjct: 123 -GNCYVSGCPGSFYTRLFPSKSLHFVHSSFCLHWLSK 155

[Close]

BLAST of Spo25251.1 vs. TAIR (Arabidopsis)
Match: AT5G38020.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 124.8 bits (312), Expect = 5.100e-29
Identity = 73/157 (46.50%), Postives = 96/157 (61.15%), Query Frame = 1


		  
Query: 8   VLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQ-PTTLCIADLGCSSAEP 67
           VL MK G  E SY   S  Q+++ S  K +  + + E+  K   P  + +ADLGCSS E 
Sbjct: 3   VLSMKGGDGEHSYANNSEGQKRLASDAKPVVVETVKEMIVKTDFPGCIKVADLGCSSGE- 62

Query: 68  NTLGVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKKQVGNN 127
           NTL V+SE+++ I  + ++ G    E    LNDLP NDFNT F+ + +F   LK  V   
Sbjct: 63  NTLLVMSEIVNTIITSYQQKGKNLPEINCCLNDLPDNDFNTTFKLVPAFHKLLKMDVK-- 122

Query: 128 FGHCFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
            G CF++GVPGSFY RLFP+K LHFVHSS  L WLS+
Sbjct: 123 -GKCFISGVPGSFYSRLFPSKSLHFVHSSLCLHWLSK 155

[Close]

BLAST of Spo25251.1 vs. TAIR (Arabidopsis)
Match: AT4G36470.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 123.6 bits (309), Expect = 1.100e-28
Identity = 69/165 (41.82%), Postives = 102/165 (61.82%), Query Frame = 1


		  
Query: 4   EVKQVLRMKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQPTTLCIADLGCSS 63
           ++++   M  G  +TSY + S +Q++     K +  + + ++Y + +P +L IADLGCSS
Sbjct: 5   DMEREFYMTGGDGKTSYARNSSLQKKASDTAKHITLETLQQLYKETRPKSLGIADLGCSS 64

Query: 64  AEPNTLGVVSELIDAIEKAR-REIG-NGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKK 123
             PNTL  +++ I  ++ A  REI      E+ ++LNDLP NDFN IF+SL  F   LK+
Sbjct: 65  G-PNTLSTITDFIKTVQVAHHREIPIQPLPEFSIFLNDLPGNDFNFIFKSLPDFHIELKR 124

Query: 124 QVGNNFGHC---FVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
              NN G C   F+   PGSFYGRLFP   +HFV++S+SL WLS+
Sbjct: 125 D--NNNGDCPSVFIAAYPGSFYGRLFPENTIHFVYASHSLHWLSK 166

[Close]

BLAST of Spo25251.1 vs. TAIR (Arabidopsis)
Match: AT2G14060.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 119.4 bits (298), Expect = 2.200e-27
Identity = 67/154 (43.51%), Postives = 94/154 (61.04%), Query Frame = 1


		  
Query: 11  MKEGTDETSYVKTSYIQRQVLSLTKSLKEKAIMEIYCKEQ-PTTLCIADLGCSSAEPNTL 70
           MK GT + SY   S+ QR V    + L  + + E+  K   P  + +ADLGCS+ + NT+
Sbjct: 1   MKGGTGDHSYATNSHYQRSVFYEIQPLVIENVREMLLKNGFPGCIKVADLGCSTGQ-NTV 60

Query: 71  GVVSELIDAIEKARREIGNGHQEYQVYLNDLPWNDFNTIFRSLGSFEDNLKKQVGNNFGH 130
             +S +   I ++ +++     E   YLNDLP NDFNT F+   SF++ LK +V    G 
Sbjct: 61  LAMSAIAYTIMESYQQMSKNPPEIDCYLNDLPENDFNTTFKLFHSFQEKLKPEVK---GK 120

Query: 131 CFVNGVPGSFYGRLFPTKHLHFVHSSYSLMWLSQ 164
            FV+GVPGSFY RLFP K LHFVHS++S+ WLS+
Sbjct: 121 WFVSGVPGSFYSRLFPRKSLHFVHSAFSIHWLSR 150

The following BLAST results are available for this feature:

BLAST of Spo25251.1 vs. NCBI nr
BLAST of Spo25251.1 vs. UniProtKB/TrEMBL
BLAST of Spo25251.1 vs. ExPASy Swiss-Prot
BLAST of Spo25251.1 vs. TAIR (Arabidopsis)

BLAST of Spo25251.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902217141\|gb\|KNA17621.1\|	4.2e-89	100.	hypothetical protein SOVF_0784...	[more]
gi\|731335426\|ref\|XP_010678722.1\|	5.6e-70	80.2	PREDICTED: salicylate carboxym...	[more]
gi\|731335424\|ref\|XP_010678721.1\|	3.1e-68	78.3	PREDICTED: salicylate carboxym...	[more]
gi\|870863842\|gb\|KMT14989.1\|	8.2e-61	69.3	hypothetical protein BVRB_3g06...	[more]
gi\|731325038\|ref\|XP_010673295.1\|	8.2e-61	69.3	PREDICTED: salicylate carboxym...	[more]

BLAST of Spo25251.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RDN0_SPIOL	2.9e-89	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CSG5_BETVU	5.7e-61	69.3	Uncharacterized protein OS=Bet...	[more]
A0A0J8CMM0_BETVU	2.4e-59	69.3	Uncharacterized protein OS=Bet...	[more]
A0A0J8FGK9_BETVU	2.4e-59	69.3	Uncharacterized protein OS=Bet...	[more]
A0A0J8CSP4_BETVU	5.3e-59	68.7	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo25251.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
SAMT_CLABR	5.5e-41	56.7	Salicylate carboxymethyltransf...	[more]
JMT_BRARP	4.0e-31	44.1	Jasmonate O-methyltransferase ...	[more]
JMT_ARATH	2.8e-29	45.5	Jasmonate O-methyltransferase ...	[more]
XMT1_COFAR	4.8e-29	41.8	7-methylxanthosine synthase 1 ...	[more]
XMT1_COFCA	4.8e-29	41.8	7-methylxanthosine synthase 1 ...	[more]

BLAST of Spo25251.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G19640.1	1.6e-30	45.5	jasmonic acid carboxyl methylt...	[more]
AT3G21950.1	1.8e-29	45.8	S-adenosyl-L-methionine-depend...	[more]
AT5G38020.1	5.1e-29	46.5	S-adenosyl-L-methionine-depend...	[more]
AT4G36470.1	1.1e-28	41.8	S-adenosyl-L-methionine-depend...	[more]
AT2G14060.1	2.2e-27	43.5	S-adenosyl-L-methionine-depend...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005299	SAM dependent carboxyl methyltransferase	PFAM	PF03492	Methyltransf_7	coord: 41..164 score: 2.1
None	No IPR available	PANTHER	PTHR31009	S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN	coord: 1..163 score: 2.9
None	No IPR available	PANTHER	PTHR31009:SF6	SUBFAMILY NOT NAMED	coord: 1..163 score: 2.9

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008168	methyltransferase activity