Spo24625.1 (mRNA)

Overview
NameSpo24625.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionrRNA intron-encoded homing endonuclease
LocationSpoScf_24737 : 174 .. 862 (-)
Sequence length492
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAAGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATAGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGNGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCT

mRNA sequence

AAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAAGATTCCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCT

Coding sequence (CDS)

AAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAAGATTCCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCT

Protein sequence

KKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFRRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAPYEKSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo24625Spo24625gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo24625.1Spo24625.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo24625.1.CDS.3Spo24625.1.CDS.3CDS
Spo24625.1.CDS.2Spo24625.1.CDS.2CDS
Spo24625.1.CDS.1Spo24625.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo24625.1.exon.3Spo24625.1.exon.3exon
Spo24625.1.exon.2Spo24625.1.exon.2exon
Spo24625.1.exon.1Spo24625.1.exon.1exon


Homology
[Close]
BLAST of Spo24625.1 vs. NCBI nr
Match: gi|703137890|ref|XP_010106597.1| (hypothetical protein L484_002552 [Morus notabilis])

HSP 1 Score: 251.9 bits (642), Expect = 7.600e-64
Identity = 130/148 (87.84%), Postives = 131/148 (88.51%), Query Frame = 1 

		  

Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFRRTTAKAF 76
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNII FRRTTAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 77  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAPYEKSKF 136
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FR          TP APYEKSKF
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFR----------TPLAPYEKSKF 174

Query: 137 LGSGGSMVARLKLKGIDGRAPPGVEPAA 165
           LGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 175 LGSGGSMVARLKLKGIDGRAPPGVEPAA 192
[Close]
BLAST of Spo24625.1 vs. NCBI nr
Match: gi|823127874|ref|XP_012440607.1| (PREDICTED: uncharacterized protein LOC105765883 [Gossypium raimondii])

HSP 1 Score: 216.1 bits (549), Expect = 4.600e-53
Identity = 116/161 (72.05%), Postives = 122/161 (75.78%), Query Frame = 1 

		  

Query: 1   KKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTL 60
           KKLVVG  GG+ GPP G HR +R + RRCAPGLNWPGR+S  VTLKKLECSKQAYA    
Sbjct: 41  KKLVVGLRGGSAGPPHGEHRSARPYCRRCAPGLNWPGRSSDAVTLKKLECSKQAYA---- 100

Query: 61  AWDNIIRFRRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAP 120
                         +FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAP
Sbjct: 101 --------------SFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAP 160

Query: 121 YEKSKTPPAPYEKSKFLGSGGSMVARLKLKGIDGRAPPGVE 162
           YEKSK+          LGSGGSMVARLKLKGIDGRAPPGVE
Sbjct: 161 YEKSKS----------LGSGGSMVARLKLKGIDGRAPPGVE 173
[Close]
BLAST of Spo24625.1 vs. NCBI nr
Match: gi|694380941|ref|XP_009366572.1| (PREDICTED: uncharacterized protein LOC103956333 [Pyrus x bretschneideri])

HSP 1 Score: 214.9 bits (546), Expect = 1.000e-52
Identity = 117/152 (76.97%), Postives = 121/152 (79.61%), Query Frame = 1 

		  

Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFR-----RT 76
           GVHR +R F RR APGLNWPGRASG VTLKKLECSKQ +    LA+   +        RT
Sbjct: 228 GVHRSARPFCRRYAPGLNWPGRASGAVTLKKLECSKQDFGPILLAFGIGVMINRDSRGRT 287

Query: 77  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAPY 136
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFR          TPPAPY
Sbjct: 288 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFR----------TPPAPY 347

Query: 137 EKSKFLGSGGSMVARLKLKGIDGRAPPGVEPA 164
           EKSKFLGSGGSMVARLKLKGIDGRAPPGVEPA
Sbjct: 348 EKSKFLGSGGSMVARLKLKGIDGRAPPGVEPA 369
[Close]
BLAST of Spo24625.1 vs. NCBI nr
Match: gi|922327155|ref|XP_013442970.1| (hypothetical protein MTR_0055s0130 [Medicago truncatula])

HSP 1 Score: 209.5 bits (532), Expect = 4.300e-51
Identity = 115/153 (75.16%), Postives = 120/153 (78.43%), Query Frame = 1 

		  

Query: 16  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFR-----R 75
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ      LA+   +        R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 76  TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAP 135
           TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK+    
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKS---- 604

Query: 136 YEKSKFLGSGGSMVARLKLKGIDGRAPPGVEPA 164
                 LGSGGSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 ------LGSGGSMVARLKLKGIDGRAPPGVEPA 627
[Close]
BLAST of Spo24625.1 vs. NCBI nr
Match: gi|947068904|gb|KRH17795.1| (hypothetical protein GLYMA_13G016700 [Glycine max])

HSP 1 Score: 206.8 bits (525), Expect = 2.800e-50
Identity = 115/153 (75.16%), Postives = 119/153 (77.78%), Query Frame = 1 

		  

Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFR-----RT 76
           GVHR +R F RRCAP LNWPGRASG VTLKKLECSKQ      LA+   +        RT
Sbjct: 128 GVHRSARPFCRRCAPVLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGRT 187

Query: 77  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAPY 136
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK+     
Sbjct: 188 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKS----- 247

Query: 137 EKSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 165
                LGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 248 -----LGSGGSMVARLKLKGIDGRAPPGVEPAA 270
[Close]
BLAST of Spo24625.1 vs. UniProtKB/TrEMBL
Match: M1DUP9_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400044276 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 6.300e-65
Identity = 132/151 (87.42%), Postives = 132/151 (87.42%), Query Frame = 1 

		  

Query: 14  PPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFRRTTA 73
           P   VHR SR F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNII FRRTTA
Sbjct: 45  PCICVHRSSRPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTA 104

Query: 74  KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAPYEK 133
           KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FR          TPPAPYEK
Sbjct: 105 KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFR----------TPPAPYEK 164

Query: 134 SKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 165
           SKFLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 165 SKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 185
[Close]
BLAST of Spo24625.1 vs. UniProtKB/TrEMBL
Match: W9SJ11_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_002552 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 5.300e-64
Identity = 130/148 (87.84%), Postives = 131/148 (88.51%), Query Frame = 1 

		  

Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFRRTTAKAF 76
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNII FRRTTAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 77  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAPYEKSKF 136
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FR          TP APYEKSKF
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFR----------TPLAPYEKSKF 174

Query: 137 LGSGGSMVARLKLKGIDGRAPPGVEPAA 165
           LGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 175 LGSGGSMVARLKLKGIDGRAPPGVEPAA 192
[Close]
BLAST of Spo24625.1 vs. UniProtKB/TrEMBL
Match: M1DB49_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400035737 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 3.600e-60
Identity = 123/140 (87.86%), Postives = 125/140 (89.29%), Query Frame = 1 

		  

Query: 25  FRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFRRTTAKAFAKDVFINQ 84
           F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNII FRRTTAKAFAKDVFINQ
Sbjct: 5   FCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAFAKDVFINQ 64

Query: 85  ERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAPYEKSKFLGSGGSMV 144
           ERKLGARRRSDTVLVSTINDADQGSADV FR          TPPAPYEKSKFLGSGGS+V
Sbjct: 65  ERKLGARRRSDTVLVSTINDADQGSADVAFR----------TPPAPYEKSKFLGSGGSIV 124

Query: 145 ARLKLKGIDGRAPPGVEPAA 165
           ARLKLKGIDG+APPGVE AA
Sbjct: 125 ARLKLKGIDGKAPPGVELAA 134
[Close]
BLAST of Spo24625.1 vs. UniProtKB/TrEMBL
Match: A0A0D2ZQ67_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 5.900e-55
Identity = 119/152 (78.29%), Postives = 123/152 (80.92%), Query Frame = 1 

		  

Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFRRTTA--- 76
           G+HR +  F R+ APGLNWPGRASG VTLKKLECSKQAY LYTLAWDNII F    A   
Sbjct: 189 GMHRSACPFCRQYAPGLNWPGRASGAVTLKKLECSKQAYTLYTLAWDNIIGFLSYCAGLQ 248

Query: 77  -KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAPYE 136
            + FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FR          TP APYE
Sbjct: 249 DRTFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFR----------TPLAPYE 308

Query: 137 KSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 165
           KSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 309 KSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 330
[Close]
BLAST of Spo24625.1 vs. UniProtKB/TrEMBL
Match: A0A072TTW7_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_0055s0130 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.000e-51
Identity = 115/153 (75.16%), Postives = 120/153 (78.43%), Query Frame = 1 

		  

Query: 16  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIRFR-----R 75
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ      LA+   +        R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 76  TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKTPPAP 135
           TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK+    
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKS---- 604

Query: 136 YEKSKFLGSGGSMVARLKLKGIDGRAPPGVEPA 164
                 LGSGGSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 ------LGSGGSMVARLKLKGIDGRAPPGVEPA 627
The following BLAST results are available for this feature:
BLAST of Spo24625.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|703137890|ref|XP_010106597.1|7.6e-6487.8hypothetical protein L484_0025... [more]
gi|823127874|ref|XP_012440607.1|4.6e-5372.0PREDICTED: uncharacterized pro... [more]
gi|694380941|ref|XP_009366572.1|1.0e-5276.9PREDICTED: uncharacterized pro... [more]
gi|922327155|ref|XP_013442970.1|4.3e-5175.1hypothetical protein MTR_0055s... [more]
gi|947068904|gb|KRH17795.1|2.8e-5075.1hypothetical protein GLYMA_13G... [more]
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BLAST of Spo24625.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
M1DUP9_SOLTU6.3e-6587.4Uncharacterized protein OS=Sol... [more]
W9SJ11_9ROSA5.3e-6487.8Uncharacterized protein OS=Mor... [more]
M1DB49_SOLTU3.6e-6087.8Uncharacterized protein OS=Sol... [more]
A0A0D2ZQ67_BRAOL5.9e-5578.2Uncharacterized protein OS=Bra... [more]
A0A072TTW7_MEDTR3.0e-5175.1Uncharacterized protein OS=Med... [more]
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BLAST of Spo24625.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo24625.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0
Match NameE-valueIdentityDescription
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34410FAMILY NOT NAMEDcoord: 59..164
score: 5.5
NoneNo IPR availablePANTHERPTHR34410:SF2SUBFAMILY NOT NAMEDcoord: 59..164
score: 5.5

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006506 GPI anchor biosynthetic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0016758 transferase activity, transferring hexosyl groups