Spo22403.1 (mRNA) | SpinachBase

Overview


Name	Spo22403.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Response to low sulfur 3, putative
Location	chr3 : 7989326 .. 7989622 (-)
Sequence length	297

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAATGATTACCAAGAAGATTATGATCTTATTAAGAAGCATAACGAAGAATTAGTGAGAAAATTAAGGGAAAGTCTAGTAAGAGAAACAAAGATTAAGGAAGAGTTGTATAGAGCATTGGAGAGGGCTAAAGTGGCAGAAGATGGAGAAGAAATGCTTTGTTCACAATTAGGAGAGCTTGAAGCCGAGTCTGTGGATCAAGCGCGAGATTTTCAAGCTCGGATGATAGCGTTGATGGATCAACTCTCCCAAGCACAAAAACTTCTTCAAGGACATTCCATTACCATGCATTAA

mRNA sequence

ATGGGAAATGATTACCAAGAAGATTATGATCTTATTAAGAAGCATAACGAAGAATTAGTGAGAAAATTAAGGGAAAGTCTAGTAAGAGAAACAAAGATTAAGGAAGAGTTGTATAGAGCATTGGAGAGGGCTAAAGTGGCAGAAGATGGAGAAGAAATGCTTTGTTCACAATTAGGAGAGCTTGAAGCCGAGTCTGTGGATCAAGCGCGAGATTTTCAAGCTCGGATGATAGCGTTGATGGATCAACTCTCCCAAGCACAAAAACTTCTTCAAGGACATTCCATTACCATGCATTAA

Coding sequence (CDS)

ATGGGAAATGATTACCAAGAAGATTATGATCTTATTAAGAAGCATAACGAAGAATTAGTGAGAAAATTAAGGGAAAGTCTAGTAAGAGAAACAAAGATTAAGGAAGAGTTGTATAGAGCATTGGAGAGGGCTAAAGTGGCAGAAGATGGAGAAGAAATGCTTTGTTCACAATTAGGAGAGCTTGAAGCCGAGTCTGTGGATCAAGCGCGAGATTTTCAAGCTCGGATGATAGCGTTGATGGATCAACTCTCCCAAGCACAAAAACTTCTTCAAGGACATTCCATTACCATGCATTAA

Protein sequence

MGNDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAESVDQARDFQARMIALMDQLSQAQKLLQGHSITMH

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo22403	Spo22403	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo22403.1	Spo22403.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22403.1.CDS.1	Spo22403.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22403.1.exon.1	Spo22403.1.exon.1	exon

Homology

[Close]

BLAST of Spo22403.1 vs. NCBI nr
Match: gi|902185990|gb|KNA10591.1| (hypothetical protein SOVF_142840 [Spinacia oleracea])

HSP 1 Score: 184.5 bits (467), Expect = 8.900e-44
Identity = 98/98 (100.00%), Postives = 98/98 (100.00%), Query Frame = 1


		  
Query: 1  MGNDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGE 60
          MGNDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGE
Sbjct: 1  MGNDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGE 60

Query: 61 LEAESVDQARDFQARMIALMDQLSQAQKLLQGHSITMH 99
          LEAESVDQARDFQARMIALMDQLSQAQKLLQGHSITMH
Sbjct: 61 LEAESVDQARDFQARMIALMDQLSQAQKLLQGHSITMH 98

[Close]

BLAST of Spo22403.1 vs. NCBI nr
Match: gi|410129770|dbj|BAM64848.1| (hypothetical protein [Beta vulgaris])

HSP 1 Score: 134.8 bits (338), Expect = 8.100e-29
Identity = 75/93 (80.65%), Postives = 86/93 (92.47%), Query Frame = 1


		  
Query: 3  NDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELE 62
          +DYQE+YDL KK NEEL RKLRES VRE KI+EELYRALER +VAE+GEEMLCSQLGELE
Sbjct: 5  SDYQENYDL-KKCNEELERKLRESQVREKKIREELYRALERVRVAEEGEEMLCSQLGELE 64

Query: 63 AESVDQARDFQARMIALMDQLSQAQKLLQGHSI 96
          AE+VDQARDF+ARM+ALM++LS+AQKLLQ HSI
Sbjct: 65 AEAVDQARDFRARMLALMEELSKAQKLLQVHSI 96

[Close]

BLAST of Spo22403.1 vs. NCBI nr
Match: gi|731321282|ref|XP_010671267.1| (PREDICTED: uncharacterized protein LOC104888103 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 134.8 bits (338), Expect = 8.100e-29
Identity = 75/93 (80.65%), Postives = 86/93 (92.47%), Query Frame = 1


		  
Query: 3  NDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELE 62
          +DYQE+YDL KK NEEL RKLRES VRE KI+EELYRALER +VAE+GEEMLCSQLGELE
Sbjct: 5  SDYQENYDL-KKCNEELERKLRESQVREKKIREELYRALERVRVAEEGEEMLCSQLGELE 64

Query: 63 AESVDQARDFQARMIALMDQLSQAQKLLQGHSI 96
          AE+VDQARDF+ARM+ALM++LS+AQKLLQ HSI
Sbjct: 65 AEAVDQARDFRARMLALMEELSKAQKLLQVHSI 96

[Close]

BLAST of Spo22403.1 vs. NCBI nr
Match: gi|661891082|emb|CDP04964.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 100.9 bits (250), Expect = 1.300e-18
Identity = 54/92 (58.70%), Postives = 75/92 (81.52%), Query Frame = 1


		  
Query: 6   QEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAES 65
           +ED +++KK NEEL ++L++++ RE +++EEL R  +R +VAE+ EE LCSQLGELEAE+
Sbjct: 22  REDGEILKKRNEELEKELQKAMEREKRLREELQRTWQRLRVAEEAEERLCSQLGELEAEA 81

Query: 66  VDQARDFQARMIALMDQLSQAQKLLQGHSITM 98
           V QAR ++A M+ALMDQLS AQKLLQG SI +
Sbjct: 82  VGQARSYRASMMALMDQLSAAQKLLQGASIAV 113

[Close]

BLAST of Spo22403.1 vs. NCBI nr
Match: gi|645257864|ref|XP_008234609.1| (PREDICTED: MAP7 domain-containing protein 1-like [Prunus mume])

HSP 1 Score: 99.8 bits (247), Expect = 2.900e-18
Identity = 56/90 (62.22%), Postives = 72/90 (80.00%), Query Frame = 1


		  
Query: 7   EDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAESV 66
           E+  L++K NEEL R+LR+S  RE ++K EL +A ER +VAE+ EE LCSQLGELEAE+V
Sbjct: 11  EEEKLLRKRNEELERELRKSQEREERMKAELQKARERLRVAEEAEERLCSQLGELEAEAV 70

Query: 67  DQARDFQARMIALMDQLSQAQKLLQGHSIT 97
           DQAR   AR++ALM+QLSQAQ+LLQ  S+T
Sbjct: 71  DQARTDHARILALMNQLSQAQRLLQASSVT 100

[Close]

BLAST of Spo22403.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QVQ2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_142840 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 6.200e-44
Identity = 98/98 (100.00%), Postives = 98/98 (100.00%), Query Frame = 1


		  
Query: 1  MGNDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGE 60
          MGNDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGE
Sbjct: 1  MGNDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGE 60

Query: 61 LEAESVDQARDFQARMIALMDQLSQAQKLLQGHSITMH 99
          LEAESVDQARDFQARMIALMDQLSQAQKLLQGHSITMH
Sbjct: 61 LEAESVDQARDFQARMIALMDQLSQAQKLLQGHSITMH 98

[Close]

BLAST of Spo22403.1 vs. UniProtKB/TrEMBL
Match: K4Q0G2_BETVU (Uncharacterized protein OS=Beta vulgaris PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 5.600e-29
Identity = 75/93 (80.65%), Postives = 86/93 (92.47%), Query Frame = 1


		  
Query: 3  NDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELE 62
          +DYQE+YDL KK NEEL RKLRES VRE KI+EELYRALER +VAE+GEEMLCSQLGELE
Sbjct: 5  SDYQENYDL-KKCNEELERKLRESQVREKKIREELYRALERVRVAEEGEEMLCSQLGELE 64

Query: 63 AESVDQARDFQARMIALMDQLSQAQKLLQGHSI 96
          AE+VDQARDF+ARM+ALM++LS+AQKLLQ HSI
Sbjct: 65 AEAVDQARDFRARMLALMEELSKAQKLLQVHSI 96

[Close]

BLAST of Spo22403.1 vs. UniProtKB/TrEMBL
Match: A0A068U9T8_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00019842001 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 9.000e-19
Identity = 54/92 (58.70%), Postives = 75/92 (81.52%), Query Frame = 1


		  
Query: 6   QEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAES 65
           +ED +++KK NEEL ++L++++ RE +++EEL R  +R +VAE+ EE LCSQLGELEAE+
Sbjct: 22  REDGEILKKRNEELEKELQKAMEREKRLREELQRTWQRLRVAEEAEERLCSQLGELEAEA 81

Query: 66  VDQARDFQARMIALMDQLSQAQKLLQGHSITM 98
           V QAR ++A M+ALMDQLS AQKLLQG SI +
Sbjct: 82  VGQARSYRASMMALMDQLSAAQKLLQGASIAV 113

[Close]

BLAST of Spo22403.1 vs. UniProtKB/TrEMBL
Match: Q0ZIH9_TOBAC (UP-9A OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.700e-17
Identity = 51/88 (57.95%), Postives = 72/88 (81.82%), Query Frame = 1


		  
Query: 10  DLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAESVDQA 69
           +++++ NEEL ++L++S+ RE K+K+EL +  ER +VAE+ EE LCSQLGELEAE+VDQA
Sbjct: 26  EILRRRNEELEKELKKSIEREEKMKQELQKTWERLRVAEEAEERLCSQLGELEAEAVDQA 85

Query: 70  RDFQARMIALMDQLSQAQKLLQGHSITM 98
           R ++ R+I LMDQLS AQKLL+  SIT+
Sbjct: 86  RTYRTRVIHLMDQLSLAQKLLESASITV 113

[Close]

BLAST of Spo22403.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CS37_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g050500 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 2.200e-17
Identity = 52/94 (55.32%), Postives = 72/94 (76.60%), Query Frame = 1


		  
Query: 3   NDYQEDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELE 62
           N   +D ++++K NEEL R+L+ESL+RE K+K +L R LER +VAE+ EE LC +LGELE
Sbjct: 23  NIRNDDVEMMRKRNEELERELKESLIREEKMKVQLERVLERVRVAEEAEERLCLELGELE 82

Query: 63  AESVDQARDFQARMIALMDQLSQAQKLLQGHSIT 97
            E+V+ AR++ AR+ ALMDQLS+A  LLQ  SI+
Sbjct: 83  VEAVENAREYNARIAALMDQLSRAHMLLQSASIS 116

[Close]

BLAST of Spo22403.1 vs. TAIR (Arabidopsis)
Match: AT3G49570.1 (response to low sulfur 3)

HSP 1 Score: 62.4 bits (150), Expect = 1.800e-10
Identity = 35/79 (44.30%), Postives = 54/79 (68.35%), Query Frame = 1


		  
Query: 7  EDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAESV 66
          E+ + +++ N EL R++ E       +K+E+ +   R  VAE+ EE LCSQL ELE ES+
Sbjct: 13 EEVEELRRRNGELEREMEE-------MKKEMVQLWRRTVVAEEAEERLCSQLAELEVESL 72

Query: 67 DQARDFQARMIALMDQLSQ 86
          DQARD+ +R++ LMDQ+S+
Sbjct: 73 DQARDYHSRIVFLMDQISR 84

[Close]

BLAST of Spo22403.1 vs. TAIR (Arabidopsis)
Match: AT3G49580.1 (response to low sulfur 1)

HSP 1 Score: 62.0 bits (149), Expect = 2.400e-10
Identity = 36/79 (45.57%), Postives = 53/79 (67.09%), Query Frame = 1


		  
Query: 7  EDYDLIKKHNEELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAESV 66
          E+ D +++ N EL R++ E       +K E+ +  +R  VAE+ EE LCSQL ELE ES+
Sbjct: 13 EEMDELRRRNIELSREVAE-------MKTEMIKLWQRTVVAEEAEEQLCSQLAELEVESL 72

Query: 67 DQARDFQARMIALMDQLSQ 86
          +QARD+  RM+ LMDQ+S+
Sbjct: 73 EQARDYHDRMLFLMDQISR 84

[Close]

BLAST of Spo22403.1 vs. TAIR (Arabidopsis)
Match: AT5G24660.1 (response to low sulfur 2)

HSP 1 Score: 60.5 bits (145), Expect = 6.900e-10
Identity = 35/69 (50.72%), Postives = 50/69 (72.46%), Query Frame = 1


		  
Query: 17 EELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAESVDQARDFQARM 76
          +EL RK  E      ++K+E+ +   R +VAE+ EE LCSQL ELEAES+DQARD+ +R+
Sbjct: 16 DELRRKNGEMEKAVEEMKKEMLQLWRRTQVAEEAEERLCSQLAELEAESLDQARDYHSRI 75

Query: 77 IALMDQLSQ 86
          I LM++LS+
Sbjct: 76 IFLMNELSR 84

[Close]

BLAST of Spo22403.1 vs. TAIR (Arabidopsis)
Match: AT5G24655.1 (response to low sulfur 4)

HSP 1 Score: 57.0 bits (136), Expect = 7.600e-9
Identity = 34/69 (49.28%), Postives = 48/69 (69.57%), Query Frame = 1


		  
Query: 17 EELVRKLRESLVRETKIKEELYRALERAKVAEDGEEMLCSQLGELEAESVDQARDFQARM 76
          EEL +K  E      ++++E+ +   R +VAE+ EE LCSQL ELEAES+DQARD+  R+
Sbjct: 16 EELRQKNGEMEKAVEEMRKEMLQLWRRTQVAEEAEEHLCSQLAELEAESLDQARDYHTRI 75

Query: 77 IALMDQLSQ 86
          I L +QLS+
Sbjct: 76 IFLTNQLSR 84

The following BLAST results are available for this feature:

BLAST of Spo22403.1 vs. NCBI nr
BLAST of Spo22403.1 vs. UniProtKB/TrEMBL
BLAST of Spo22403.1 vs. ExPASy Swiss-Prot
BLAST of Spo22403.1 vs. TAIR (Arabidopsis)

BLAST of Spo22403.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902185990\|gb\|KNA10591.1\|	8.9e-44	100.	hypothetical protein SOVF_1428...	[more]
gi\|410129770\|dbj\|BAM64848.1\|	8.1e-29	80.6	hypothetical protein [Beta vul...	[more]
gi\|731321282\|ref\|XP_010671267.1\|	8.1e-29	80.6	PREDICTED: uncharacterized pro...	[more]
gi\|661891082\|emb\|CDP04964.1\|	1.3e-18	58.7	unnamed protein product [Coffe...	[more]
gi\|645257864\|ref\|XP_008234609.1\|	2.9e-18	62.2	PREDICTED: MAP7 domain-contain...	[more]

BLAST of Spo22403.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QVQ2_SPIOL	6.2e-44	100.	Uncharacterized protein OS=Spi...	[more]
K4Q0G2_BETVU	5.6e-29	80.6	Uncharacterized protein OS=Bet...	[more]
A0A068U9T8_COFCA	9.0e-19	58.7	Uncharacterized protein OS=Cof...	[more]
Q0ZIH9_TOBAC	1.7e-17	57.9	UP-9A OS=Nicotiana tabacum PE=...	[more]
A0A0J8CS37_BETVU	2.2e-17	55.3	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo22403.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo22403.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4


Match Name	E-value	Identity	Description
AT3G49570.1	1.8e-10	44.3	response to low sulfur 3	[more]
AT3G49580.1	2.4e-10	45.5	response to low sulfur 1	[more]
AT5G24660.1	6.9e-10	50.7	response to low sulfur 2	[more]
AT5G24655.1	7.6e-9	49.2	response to low sulfur 4	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34283	FAMILY NOT NAMED	coord: 7..98 score: 5.6
None	No IPR available	PANTHER	PTHR34283:SF1	SUBFAMILY NOT NAMED	coord: 7..98 score: 5.6

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000910	cytokinesis
biological_process	GO:0000226	microtubule cytoskeleton organization
cellular_component	GO:0045298	tubulin complex
molecular_function	GO:0008017	microtubule binding