Spo21748.1 (mRNA)

Overview
NameSpo21748.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionProbable calcium-binding protein CML21 (Calmodulin-like protein 21)
Locationchr2 : 56546245 .. 56549371 (-)
Sequence length1426
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAAGGTTATGGTATATTCTAAATTTGAACCTTCAAATTCCAAAATCCAAAAGAAAAGAAAAAGACTGGACAATACGTGGTGGGTGCAGCACAAACCCCGGCTACCTATCTTCTGAACAATCAAAGTTCATCAAAAAAAAAAATCAAAACTTGAATAATCAAAGTGATCCCATTCTCTTTCTCTGTGTTCAAACATCATACTATAATCATATTCATTACTCCCAAATCTTCAACATAATCTTGTTTCTGGAACAACATTCATACTATAAGCATCAGGAAGATAAGAACAAGACTCTGATTTACAATTCTCTTATCCTTTGGTTATTCCAATCAATCAATCAATCAGGTGATTTTTTAATTTCATTTCATAATTTCATTTTGTTGGTTGGTTTAATAGCTTATTCGATTTAATTCGATTTGGTGATTGCAGAATCAATTGAATTGAAGATGGGAGGTTCGTTAGGGGTAGTTGCAGAATCACCAAAAAGAGGTTATTTCCCAGAAACCAAACTCGAAACTAAAATGGTGGAAGCTATGCAAAATAGGGCTTCCCCCATGAAATCCTTCAACACCATCATTCTTAAATTTCCTAAAATTGATCAGAGTTTTAGGAAATGCAAAGCAATCTTCGAACAATTTGGTAAAATCAATCAACCAACCCCTTTTTAATTCATCTGTTTTCAATCTATTATTCATTCTTGTTTTTTAATTGAATTGGTATCGAATCCTGATTATGGCAGATGTGGATAAGAATGAGGTGATTGATCACAGTGAGCTGAAACACTGCTTACGAAAACTGGAAGTTATCTTCAGTGAGGAAGAGATTGATGATTTCTTCAAATCATGTGATATAAATGAGAATATGGGGATTAAATTCAATGAGTTTATTGTTCTTCTTTGTCTTGTTTACCTTCTTCAGGAGGATCCTTCCAAGAAGACTCAACTTGTATCCTTTTTTAATTTAATTTTAATTTAATTTCTGTTCTTCAATTGAAATATTTGAATCATGATTTTGCTTTAACATTCAATTTTACCTTAGAAATCTCGGATGGGTTTGCCCAATTTGGAGGCCACATTTGAAACTCTTGTTGATGCTTTTGCTTTTTTGGACAAAAATAAAGATGGTTATGTCAGCAAGACTGAAATGGTTCAGGCCATTAATGAAACTACCACTGGGGAACGTTCTTCTGGACGAATCGCCATGAAAAGATTTGGTCAGCTTCCCAACCCCTCCTTTTGCTTTCTCTTTACTGTTCATCATTAAGCCTCTGTTTAGTTTGGCGCAAAACGTTTTCAGTGAAAAAGGGTTTTCCAAATTCAAAACTAGTTTTCTCTGTTTGGTTCGTTGCAAGAAAATTAATAGGAAAAGGAAAATGTGAGGAGACGAGAGAAGATTGATGGGAAGAAAGGAAGAGGGAGGTAAGAAAGAAAGAAAGTGGGAAAGTAGTTTTTCCACGTTTGGGAGAGTTATGAAAAGTTGTTTCTCATCCCTTGTCAAAACCAAACATGTAAAATGATTGAAAAGGGGAAAATGTAACCAAACGGAGCCTAAATCATATACGCTATCTATGTGCTATTACAGCATTTGGGAATTACATTTTAACTGAATTCATCAATTTTCTGCTGTAATCTTGATGGGTTCGATTTGAAGTGTGGTATAGTCTAAATTGATACTCCTTAAAAGCCAACTTGGGAACAAGGACTTGATGCTTGACTGGCTGGACTGAGGTTCTAAGTCTATACTTTGAATTTATTTGGTCGTGTCCAGGTATTATGTTCTGTCCTGGTGTCTGAGACTTACCTGCAGGTGCTTTTACATTTTCTACCTATAGAGAAAACTAAACATGGTGATGAGTTTGGTTGTATCCCGCATATCATGTCCTCGTGTCTGAGACTTATCCTCTGTTTGGTTTGGCGTAAAACATTTTCAATGAAAAATGATTTTTCACGGAAAACCTTTTTCAAAGAAAAACACAATTCCAAACTAGTTTCCTTTGTTTGGTTCCTTACAAGAAAATTAATAGAAAAAAGAAAAATGGGAGAAGATAGGTGAAGATAGGTGAAGATTAATGGAAGAAGGAAAATGGAGGTAAGGAGGTGAGGAAAGATGGAAAAGTGTTCCCTTCCTTTCAAATGGAAAATGTTTTTCCACCTTTGAGGGAGTCATGGAAAATTGATTTGCATCTCTTGTCAAACCAAACAACGTAAAGTGACTGAAAAGGGGAAAACTGTTTTCCATGAAAACGTTTTACACCCTACCAAACGGAACCTTGTTACTTTGCATTGTCTATCGGTCTATCCATAGAGAAGTGTAAGGACGACATTGAGTTTTACAAGGCACTCAGGAAAAATCATCTCTCCCACAACAAGTTTTAAGTTTAATGTTGATTAGTGTGAGAAATTAAACCAGAAGTGTTATGTTTTATGACATTGGTGTGTTCAGAATTGATATGATTGTGTTTAGAATTTGCAAATCAAAGAATCATCTTCTTCAGAGCTTGCCAAGCTCTGCATTTGTAGATTTTAATGTGTTGTTGTGCTACTGCAGAAGAGATGGACTGGGATAGAAATGGAATGGTTAACTTCAAAGAATTTCTATTTGCCTTCACCCGTTGGGTTGGTATCGATGAAGCTGAAGACGAGGATGAAGTAGATGAGGGTGAAGGTGAAGGAGAAGCTTAATCCTTTACTTTCTTGGAACTTCCTCTATAGTTTTCTATGATATGAAATACCTGTAAGTCTGTAACAGCAAATAGTTGAGCTGGTTAGGTGGATGGTGTTTACACCTTAGACTCTTAATTTTGACAAAATGTTGAGTTGTTATACAAAGGCATGGCTGCTGTTTTTTTCCCCACAGCCCATCACCACTTGTGTGCAGCAACCCAGTACAGCTTTAATAAGTGTAGCATGATACTTTTTGAAGGTCTTTAGTTTTGCATTTCATTGTCTGTACTTTAAGTTTGATACAGGTTTTGTAGTTTGTGACTATAGTGCTCTGCTTTGAACCATAGTATGAAACTCAAAAGTATGGAACTACTACCTCCAACTCTCCAAGGCTCCAATTCAAAAGACTGCATTTATATTCTAGTGTCCTAGTTCAAATTGTTGTGCCTTCCAGAATCTTCC

mRNA sequence

GTTAAGGTTATGGTATATTCTAAATTTGAACCTTCAAATTCCAAAATCCAAAAGAAAAGAAAAAGACTGGACAATACGTGGTGGGTGCAGCACAAACCCCGGCTACCTATCTTCTGAACAATCAAAGTTCATCAAAAAAAAAAATCAAAACTTGAATAATCAAAGTGATCCCATTCTCTTTCTCTGTGTTCAAACATCATACTATAATCATATTCATTACTCCCAAATCTTCAACATAATCTTGTTTCTGGAACAACATTCATACTATAAGCATCAGGAAGATAAGAACAAGACTCTGATTTACAATTCTCTTATCCTTTGGTTATTCCAATCAATCAATCAATCAGAATCAATTGAATTGAAGATGGGAGGTTCGTTAGGGGTAGTTGCAGAATCACCAAAAAGAGGTTATTTCCCAGAAACCAAACTCGAAACTAAAATGGTGGAAGCTATGCAAAATAGGGCTTCCCCCATGAAATCCTTCAACACCATCATTCTTAAATTTCCTAAAATTGATCAGAGTTTTAGGAAATGCAAAGCAATCTTCGAACAATTTGATGTGGATAAGAATGAGGTGATTGATCACAGTGAGCTGAAACACTGCTTACGAAAACTGGAAGTTATCTTCAGTGAGGAAGAGATTGATGATTTCTTCAAATCATGTGATATAAATGAGAATATGGGGATTAAATTCAATGAGTTTATTGTTCTTCTTTGTCTTGTTTACCTTCTTCAGGAGGATCCTTCCAAGAAGACTCAACTTAAATCTCGGATGGGTTTGCCCAATTTGGAGGCCACATTTGAAACTCTTGTTGATGCTTTTGCTTTTTTGGACAAAAATAAAGATGAAGAGATGGACTGGGATAGAAATGGAATGGTTAACTTCAAAGAATTTCTATTTGCCTTCACCCGTTGGGTTGGTATCGATGAAGCTGAAGACGAGGATGAAGTAGATGAGGGTGAAGGTGAAGGAGAAGCTTAATCCTTTACTTTCTTGGAACTTCCTCTATAGTTTTCTATGATATGAAATACCTGTAAGTCTGTAACAGCAAATAGTTGAGCTGGTTAGGTGGATGGTGTTTACACCTTAGACTCTTAATTTTGACAAAATGTTGAGTTGTTATACAAAGGCATGGCTGCTGTTTTTTTCCCCACAGCCCATCACCACTTGTGTGCAGCAACCCAGTACAGCTTTAATAAGTGTAGCATGATACTTTTTGAAGGTCTTTAGTTTTGCATTTCATTGTCTGTACTTTAAGTTTGATACAGGTTTTGTAGTTTGTGACTATAGTGCTCTGCTTTGAACCATAGTATGAAACTCAAAAGTATGGAACTACTACCTCCAACTCTCCAAGGCTCCAATTCAAAAGACTGCATTTATATTCTAGTGTCCTAGTTCAAATTGTTGTGCCTTCCAGAATCTTCC

Coding sequence (CDS)

ATGGGAGGTTCGTTAGGGGTAGTTGCAGAATCACCAAAAAGAGGTTATTTCCCAGAAACCAAACTCGAAACTAAAATGGTGGAAGCTATGCAAAATAGGGCTTCCCCCATGAAATCCTTCAACACCATCATTCTTAAATTTCCTAAAATTGATCAGAGTTTTAGGAAATGCAAAGCAATCTTCGAACAATTTGATGTGGATAAGAATGAGGTGATTGATCACAGTGAGCTGAAACACTGCTTACGAAAACTGGAAGTTATCTTCAGTGAGGAAGAGATTGATGATTTCTTCAAATCATGTGATATAAATGAGAATATGGGGATTAAATTCAATGAGTTTATTGTTCTTCTTTGTCTTGTTTACCTTCTTCAGGAGGATCCTTCCAAGAAGACTCAACTTAAATCTCGGATGGGTTTGCCCAATTTGGAGGCCACATTTGAAACTCTTGTTGATGCTTTTGCTTTTTTGGACAAAAATAAAGATGAAGAGATGGACTGGGATAGAAATGGAATGGTTAACTTCAAAGAATTTCTATTTGCCTTCACCCGTTGGGTTGGTATCGATGAAGCTGAAGACGAGGATGAAGTAGATGAGGGTGAAGGTGAAGGAGAAGCTTAA

Protein sequence

MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASPMKSFNTIILKFPKIDQSFRKCKAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLVYLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKDEEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGEA
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo21748Spo21748gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo21748.1Spo21748.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo21748.1.utr5p.2Spo21748.1.utr5p.2five_prime_UTR
Spo21748.1.utr5p.1Spo21748.1.utr5p.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo21748.1.CDS.4Spo21748.1.CDS.4CDS
Spo21748.1.CDS.3Spo21748.1.CDS.3CDS
Spo21748.1.CDS.2Spo21748.1.CDS.2CDS
Spo21748.1.CDS.1Spo21748.1.CDS.1CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo21748.1.utr3p.1Spo21748.1.utr3p.1three_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo21748.1.exon.5Spo21748.1.exon.5exon
Spo21748.1.exon.4Spo21748.1.exon.4exon
Spo21748.1.exon.3Spo21748.1.exon.3exon
Spo21748.1.exon.2Spo21748.1.exon.2exon
Spo21748.1.exon.1Spo21748.1.exon.1exon


Homology
[Close]
BLAST of Spo21748.1 vs. NCBI nr
Match: gi|902141404|gb|KNA04701.1| (hypothetical protein SOVF_197240 isoform A [Spinacia oleracea])

HSP 1 Score: 379.8 bits (974), Expect = 3.000e-102
Identity = 199/235 (84.68%), Postives = 200/235 (85.11%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASPMKSFNTIILKFPKIDQSFRKCKAI 60
           MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASPMKSFNTIILKFPKIDQSFRKCKAI
Sbjct: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASPMKSFNTIILKFPKIDQSFRKCKAI 60

Query: 61  FEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLV 120
           FEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLV
Sbjct: 61  FEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLV 120

Query: 121 YLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKDEEMDWD------------- 180
           YLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD  +                
Sbjct: 121 YLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKDGYVSKTEMVQAINETTTGE 180

Query: 181 -----------------RNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGEA 206
                            RNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGEA
Sbjct: 181 RSSGRIAMKRFEEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGEA 235
[Close]
BLAST of Spo21748.1 vs. NCBI nr
Match: gi|731330442|ref|XP_010676135.1| (PREDICTED: probable calcium-binding protein CML21 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 305.8 bits (782), Expect = 5.600e-80
Identity = 166/228 (72.81%), Postives = 177/228 (77.63%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASP---MKSFNTIILKFPKIDQSFRKC 60
           MGG+LGV  ESPKRGY PETKLE KMVEAMQ RAS    MKSFNTIILKFPKIDQSFRKC
Sbjct: 2   MGGALGV-PESPKRGYLPETKLEAKMVEAMQERASKGTVMKSFNTIILKFPKIDQSFRKC 61

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIFE+FD DKNEVIDH+ELKHC RKLE+ F+EEE+DDFFK+CDINENMG+KFNEFIVLL
Sbjct: 62  KAIFEEFDEDKNEVIDHNELKHCFRKLEISFTEEEVDDFFKACDINENMGMKFNEFIVLL 121

Query: 121 CLVYLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD-------------EEM 180
           CLVYLLQED   KT +KSRMGLPNLEATFETLVDAFAFLDKNKD             E  
Sbjct: 122 CLVYLLQED-QPKTGVKSRMGLPNLEATFETLVDAFAFLDKNKDGYVSKTEMIQAINETT 181

Query: 181 DWDRNG-----------------MVNFKEFLFAFTRWVGIDEAEDEDE 196
             +R+                  MVNFKEFLFAFTRWVGIDEAEDEDE
Sbjct: 182 SGERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGIDEAEDEDE 227
[Close]
BLAST of Spo21748.1 vs. NCBI nr
Match: gi|147819723|emb|CAN71481.1| (hypothetical protein VITISV_006244 [Vitis vinifera])

HSP 1 Score: 282.7 bits (722), Expect = 5.000e-73
Identity = 150/239 (62.76%), Postives = 174/239 (72.80%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASP---MKSFNTIILKFPKIDQSFRKC 60
           MGG +G   +SP++ + PETKLE KMVEAMQ RAS    MKSFN+IILKFPKID+S R C
Sbjct: 1   MGGVVGK-GDSPRKAWVPETKLEAKMVEAMQRRASEGSTMKSFNSIILKFPKIDESLRNC 60

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIF+QFD D N  IDH ELK C  KLE+ FSEEEI+D F++CDINE+MG+KFNEFIVLL
Sbjct: 61  KAIFQQFDEDSNGAIDHEELKRCFLKLEISFSEEEINDLFEACDINEDMGMKFNEFIVLL 120

Query: 121 CLVYLLQEDPS--KKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD-------------- 180
           CLVYLL+EDP+  +    KSRMG+PNLEATFETLVDAF FLDKNKD              
Sbjct: 121 CLVYLLKEDPTALQAVSKKSRMGMPNLEATFETLVDAFVFLDKNKDGYVSKSEMVHAINE 180

Query: 181 ----------------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGE 205
                           EEMDWD+NGMVNFKEFLFAFTRWVGID+ EDE+E D G+ +G+
Sbjct: 181 TTAGERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGIDDIEDEEEEDPGQIDGD 238
[Close]
BLAST of Spo21748.1 vs. NCBI nr
Match: gi|225462035|ref|XP_002268527.1| (PREDICTED: probable calcium-binding protein CML21 isoform X2 [Vitis vinifera])

HSP 1 Score: 281.2 bits (718), Expect = 1.500e-72
Identity = 151/233 (64.81%), Postives = 171/233 (73.39%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASP---MKSFNTIILKFPKIDQSFRKC 60
           MGG +G   +SP++ + PETKLE KMVEAMQ RAS    MKSFN+IILKFPKID+S R C
Sbjct: 1   MGGVVGK-GDSPRKAWVPETKLEAKMVEAMQRRASEGSTMKSFNSIILKFPKIDESLRNC 60

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIF+QFD D N  IDH ELK C  KLE+ FSEEEI+D F++CDINE+MG+KFNEFIVLL
Sbjct: 61  KAIFQQFDEDSNGAIDHEELKRCFLKLEISFSEEEINDLFEACDINEDMGMKFNEFIVLL 120

Query: 121 CLVYLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD---------------- 180
           CLVYLL+EDP+   Q KSRMG+PNLEATFETLVDAF FLDKNKD                
Sbjct: 121 CLVYLLKEDPAA-LQAKSRMGMPNLEATFETLVDAFVFLDKNKDGYVSKSEMVHAINETT 180

Query: 181 --------------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGE 201
                         EEMDWD+NGMVNFKEFLFAFTRWVGID+ EDE+E  EGE
Sbjct: 181 AGERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGIDDIEDEEE--EGE 229
[Close]
BLAST of Spo21748.1 vs. NCBI nr
Match: gi|731433404|ref|XP_010644632.1| (PREDICTED: probable calcium-binding protein CML21 isoform X1 [Vitis vinifera])

HSP 1 Score: 279.3 bits (713), Expect = 5.600e-72
Identity = 150/235 (63.83%), Postives = 171/235 (72.77%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASP---MKSFNTIILKFPKIDQSFRKC 60
           MGG +G   +SP++ + PETKLE KMVEAMQ RAS    MKSFN+IILKFPKID+S R C
Sbjct: 1   MGGVVGK-GDSPRKAWVPETKLEAKMVEAMQRRASEGSTMKSFNSIILKFPKIDESLRNC 60

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIF+QFD D N  IDH ELK C  KLE+ FSEEEI+D F++CDINE+MG+KFNEFIVLL
Sbjct: 61  KAIFQQFDEDSNGAIDHEELKRCFLKLEISFSEEEINDLFEACDINEDMGMKFNEFIVLL 120

Query: 121 CLVYLLQEDPS--KKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD-------------- 180
           CLVYLL+EDP+  +    KSRMG+PNLEATFETLVDAF FLDKNKD              
Sbjct: 121 CLVYLLKEDPAALQAVSKKSRMGMPNLEATFETLVDAFVFLDKNKDGYVSKSEMVHAINE 180

Query: 181 ----------------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGE 201
                           EEMDWD+NGMVNFKEFLFAFTRWVGID+ EDE+E  EGE
Sbjct: 181 TTAGERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGIDDIEDEEE--EGE 232
[Close]
BLAST of Spo21748.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QDV3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_197240 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 2.100e-102
Identity = 199/235 (84.68%), Postives = 200/235 (85.11%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASPMKSFNTIILKFPKIDQSFRKCKAI 60
           MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASPMKSFNTIILKFPKIDQSFRKCKAI
Sbjct: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASPMKSFNTIILKFPKIDQSFRKCKAI 60

Query: 61  FEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLV 120
           FEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLV
Sbjct: 61  FEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLV 120

Query: 121 YLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKDEEMDWD------------- 180
           YLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD  +                
Sbjct: 121 YLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKDGYVSKTEMVQAINETTTGE 180

Query: 181 -----------------RNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGEA 206
                            RNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGEA
Sbjct: 181 RSSGRIAMKRFEEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGEA 235
[Close]
BLAST of Spo21748.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CKR1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g091210 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 3.900e-80
Identity = 166/228 (72.81%), Postives = 177/228 (77.63%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASP---MKSFNTIILKFPKIDQSFRKC 60
           MGG+LGV  ESPKRGY PETKLE KMVEAMQ RAS    MKSFNTIILKFPKIDQSFRKC
Sbjct: 2   MGGALGV-PESPKRGYLPETKLEAKMVEAMQERASKGTVMKSFNTIILKFPKIDQSFRKC 61

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIFE+FD DKNEVIDH+ELKHC RKLE+ F+EEE+DDFFK+CDINENMG+KFNEFIVLL
Sbjct: 62  KAIFEEFDEDKNEVIDHNELKHCFRKLEISFTEEEVDDFFKACDINENMGMKFNEFIVLL 121

Query: 121 CLVYLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD-------------EEM 180
           CLVYLLQED   KT +KSRMGLPNLEATFETLVDAFAFLDKNKD             E  
Sbjct: 122 CLVYLLQED-QPKTGVKSRMGLPNLEATFETLVDAFAFLDKNKDGYVSKTEMIQAINETT 181

Query: 181 DWDRNG-----------------MVNFKEFLFAFTRWVGIDEAEDEDE 196
             +R+                  MVNFKEFLFAFTRWVGIDEAEDEDE
Sbjct: 182 SGERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGIDEAEDEDE 227
[Close]
BLAST of Spo21748.1 vs. UniProtKB/TrEMBL
Match: A5AMZ2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006244 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 3.500e-73
Identity = 150/239 (62.76%), Postives = 174/239 (72.80%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASP---MKSFNTIILKFPKIDQSFRKC 60
           MGG +G   +SP++ + PETKLE KMVEAMQ RAS    MKSFN+IILKFPKID+S R C
Sbjct: 1   MGGVVGK-GDSPRKAWVPETKLEAKMVEAMQRRASEGSTMKSFNSIILKFPKIDESLRNC 60

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIF+QFD D N  IDH ELK C  KLE+ FSEEEI+D F++CDINE+MG+KFNEFIVLL
Sbjct: 61  KAIFQQFDEDSNGAIDHEELKRCFLKLEISFSEEEINDLFEACDINEDMGMKFNEFIVLL 120

Query: 121 CLVYLLQEDPS--KKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD-------------- 180
           CLVYLL+EDP+  +    KSRMG+PNLEATFETLVDAF FLDKNKD              
Sbjct: 121 CLVYLLKEDPTALQAVSKKSRMGMPNLEATFETLVDAFVFLDKNKDGYVSKSEMVHAINE 180

Query: 181 ----------------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGEGEGE 205
                           EEMDWD+NGMVNFKEFLFAFTRWVGID+ EDE+E D G+ +G+
Sbjct: 181 TTAGERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGIDDIEDEEEEDPGQIDGD 238
[Close]
BLAST of Spo21748.1 vs. UniProtKB/TrEMBL
Match: D7UAN9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g01200 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.000e-72
Identity = 151/233 (64.81%), Postives = 171/233 (73.39%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRASP---MKSFNTIILKFPKIDQSFRKC 60
           MGG +G   +SP++ + PETKLE KMVEAMQ RAS    MKSFN+IILKFPKID+S R C
Sbjct: 1   MGGVVGK-GDSPRKAWVPETKLEAKMVEAMQRRASEGSTMKSFNSIILKFPKIDESLRNC 60

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIF+QFD D N  IDH ELK C  KLE+ FSEEEI+D F++CDINE+MG+KFNEFIVLL
Sbjct: 61  KAIFQQFDEDSNGAIDHEELKRCFLKLEISFSEEEINDLFEACDINEDMGMKFNEFIVLL 120

Query: 121 CLVYLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD---------------- 180
           CLVYLL+EDP+   Q KSRMG+PNLEATFETLVDAF FLDKNKD                
Sbjct: 121 CLVYLLKEDPAA-LQAKSRMGMPNLEATFETLVDAFVFLDKNKDGYVSKSEMVHAINETT 180

Query: 181 --------------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGE 201
                         EEMDWD+NGMVNFKEFLFAFTRWVGID+ EDE+E  EGE
Sbjct: 181 AGERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGIDDIEDEEE--EGE 229
[Close]
BLAST of Spo21748.1 vs. UniProtKB/TrEMBL
Match: A0A151S7V2_CAJCA (Putative calcium-binding protein CML21 OS=Cajanus cajan GN=KK1_027324 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.100e-70
Identity = 143/230 (62.17%), Postives = 167/230 (72.61%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNR---ASPMKSFNTIILKFPKIDQSFRKC 60
           MGG+LG   +SPK+G+ PETK+E KMVEAMQ R    S +KSFNTIILKFPKID+S RKC
Sbjct: 1   MGGALGK-DDSPKKGWMPETKVEAKMVEAMQRRESQGSSVKSFNTIILKFPKIDESLRKC 60

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIFEQFD D N  ID  ELK C  KLE+ F+EEEI+D F++CDIN++MG+KFNEFIVLL
Sbjct: 61  KAIFEQFDEDSNGAIDPEELKKCFSKLEISFTEEEINDLFEACDINDDMGMKFNEFIVLL 120

Query: 121 CLVYLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD---------------- 180
           CLVYLL++DP+     KSR+G+PNLEATFETLVD F FLDKNKD                
Sbjct: 121 CLVYLLKDDPAA-LHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKNEMVQAINETT 180

Query: 181 --------------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVD 198
                         EEMDWD+NGMVNFKEFLFAFTRWVGID+ EDE+  +
Sbjct: 181 SGERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGIDDIEDEENAE 228
[Close]
BLAST of Spo21748.1 vs. ExPASy Swiss-Prot
Match: CML21_ARATH (Probable calcium-binding protein CML21 OS=Arabidopsis thaliana GN=CML21 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.000e-66
Identity = 134/231 (58.01%), Postives = 163/231 (70.56%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRAS---PMKSFNTIILKFPKIDQSFRKC 60
           MGG++   +E+ ++ + PETKLE K++EA+Q RAS    MKSFN+I+LKFPKID   R C
Sbjct: 1   MGGAV-TKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNC 60

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIF++FD D N  IDH+ELK+C+RKLE+ F EEEI+D FK+CDINE+MGI F EFIVLL
Sbjct: 61  KAIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLL 120

Query: 121 CLVYLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD---------------- 180
           CLVYLL++D S   Q K  MG+P LE TFETLVD F FLD+NKD                
Sbjct: 121 CLVYLLKDD-SSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDESG 180

Query: 181 ------------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGE 201
                       EEMDWD+NGMVNFKEFLFAFT+WVGIDE E+E+E D  E
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTQWVGIDENEEEEEEDNNE 229
[Close]
BLAST of Spo21748.1 vs. ExPASy Swiss-Prot
Match: CML22_ARATH (Probable calcium-binding protein CML22 OS=Arabidopsis thaliana GN=CML22 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 6.400e-36
Identity = 85/220 (38.64%), Postives = 125/220 (56.82%), Query Frame = 1 

		  

Query: 7   VVAESPKRGYFPETKLETKMVEAMQNRAS--PMKSFNTIILKFPKIDQSFRKCKAIFEQF 66
           V +E+ K+    + KL  KMVE+         +KS ++II+KFPK+ +  R  +++FE +
Sbjct: 6   VNSENNKKYAELDAKLARKMVESRSIYPGHRSLKSMDSIIMKFPKLREGLRNIRSVFESY 65

Query: 67  DVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLVYLLQ 126
           D D N  ID  ELK CL +L++  S+EE+   +  CD++ + GI+FNEFIVLLCL+YLL 
Sbjct: 66  DNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLA 125

Query: 127 EDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDK----------------NKD------- 186
           +  S+ +     MG   +E+ F+ +V+ F FLDK                N+D       
Sbjct: 126 KPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYPLERSP 185

Query: 187 --------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDE 194
                   EEMDW R G V F+EFLFAF  WVG+D+A+D+
Sbjct: 186 SHVTNMRFEEMDWGRKGKVGFREFLFAFMSWVGLDDADDD 225
[Close]
BLAST of Spo21748.1 vs. TAIR (Arabidopsis)
Match: AT4G26470.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 253.4 bits (646), Expect = 1.200e-67
Identity = 134/231 (58.01%), Postives = 163/231 (70.56%), Query Frame = 1 

		  

Query: 1   MGGSLGVVAESPKRGYFPETKLETKMVEAMQNRAS---PMKSFNTIILKFPKIDQSFRKC 60
           MGG++   +E+ ++ + PETKLE K++EA+Q RAS    MKSFN+I+LKFPKID   R C
Sbjct: 1   MGGAV-TKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNC 60

Query: 61  KAIFEQFDVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLL 120
           KAIF++FD D N  IDH+ELK+C+RKLE+ F EEEI+D FK+CDINE+MGI F EFIVLL
Sbjct: 61  KAIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLL 120

Query: 121 CLVYLLQEDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDKNKD---------------- 180
           CLVYLL++D S   Q K  MG+P LE TFETLVD F FLD+NKD                
Sbjct: 121 CLVYLLKDD-SSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDESG 180

Query: 181 ------------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDEDEVDEGE 201
                       EEMDWD+NGMVNFKEFLFAFT+WVGIDE E+E+E D  E
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTQWVGIDENEEEEEEDNNE 229
[Close]
BLAST of Spo21748.1 vs. TAIR (Arabidopsis)
Match: AT3G24110.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 152.1 bits (383), Expect = 3.600e-37
Identity = 85/220 (38.64%), Postives = 125/220 (56.82%), Query Frame = 1 

		  

Query: 7   VVAESPKRGYFPETKLETKMVEAMQNRAS--PMKSFNTIILKFPKIDQSFRKCKAIFEQF 66
           V +E+ K+    + KL  KMVE+         +KS ++II+KFPK+ +  R  +++FE +
Sbjct: 6   VNSENNKKYAELDAKLARKMVESRSIYPGHRSLKSMDSIIMKFPKLREGLRNIRSVFESY 65

Query: 67  DVDKNEVIDHSELKHCLRKLEVIFSEEEIDDFFKSCDINENMGIKFNEFIVLLCLVYLLQ 126
           D D N  ID  ELK CL +L++  S+EE+   +  CD++ + GI+FNEFIVLLCL+YLL 
Sbjct: 66  DNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLA 125

Query: 127 EDPSKKTQLKSRMGLPNLEATFETLVDAFAFLDK----------------NKD------- 186
           +  S+ +     MG   +E+ F+ +V+ F FLDK                N+D       
Sbjct: 126 KPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYPLERSP 185

Query: 187 --------EEMDWDRNGMVNFKEFLFAFTRWVGIDEAEDE 194
                   EEMDW R G V F+EFLFAF  WVG+D+A+D+
Sbjct: 186 SHVTNMRFEEMDWGRKGKVGFREFLFAFMSWVGLDDADDD 225
The following BLAST results are available for this feature:
BLAST of Spo21748.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902141404|gb|KNA04701.1|3.0e-10284.6hypothetical protein SOVF_1972... [more]
gi|731330442|ref|XP_010676135.1|5.6e-8072.8PREDICTED: probable calcium-bi... [more]
gi|147819723|emb|CAN71481.1|5.0e-7362.7hypothetical protein VITISV_00... [more]
gi|225462035|ref|XP_002268527.1|1.5e-7264.8PREDICTED: probable calcium-bi... [more]
gi|731433404|ref|XP_010644632.1|5.6e-7263.8PREDICTED: probable calcium-bi... [more]
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BLAST of Spo21748.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QDV3_SPIOL2.1e-10284.6Uncharacterized protein OS=Spi... [more]
A0A0J8CKR1_BETVU3.9e-8072.8Uncharacterized protein OS=Bet... [more]
A5AMZ2_VITVI3.5e-7362.7Putative uncharacterized prote... [more]
D7UAN9_VITVI1.0e-7264.8Putative uncharacterized prote... [more]
A0A151S7V2_CAJCA2.1e-7062.1Putative calcium-binding prote... [more]
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BLAST of Spo21748.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2
Match NameE-valueIdentityDescription
CML21_ARATH2.0e-6658.0Probable calcium-binding prote... [more]
CML22_ARATH6.4e-3638.6Probable calcium-binding prote... [more]
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BLAST of Spo21748.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2
Match NameE-valueIdentityDescription
AT4G26470.11.2e-6758.0Calcium-binding EF-hand family... [more]
AT3G24110.13.6e-3738.6Calcium-binding EF-hand family... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 55..115
score: 9.
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 163..183
score: 0
IPR002048EF-hand domainSMARTSM00054efh_1coord: 56..84
score: 0.0061coord: 92..120
score: 8.4coord: 148..184
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 52..87
score: 11.026coord: 88..123
score: 8.851coord: 162..187
score: 7
IPR011992EF-hand domain pairGENE3D1.10.238.10coord: 22..184
score: 4.9
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 54..188
score: 9.06
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 135..193
score: 1.4E-29coord: 26..117
score: 1.4
NoneNo IPR availablePANTHERPTHR10891:SF574CALCIUM-BINDING PROTEIN CML30-RELATEDcoord: 26..117
score: 1.4E-29coord: 135..193
score: 1.4

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044237 cellular metabolic process
biological_process GO:0016043 cellular component organization
biological_process GO:0009987 cellular process
biological_process GO:0006811 ion transport
molecular_function GO:0005509 calcium ion binding