Spo19694.1 (mRNA) | SpinachBase

Overview


Name	Spo19694.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Calcium binding protein
Location	chr5 : 22511956 .. 22512615 (-)
Sequence length	660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAAGTATCAAGTGATGAAATAATAATAATAATGGATAATAAGGCAGAGAAGTCGAAGAGGATGCTTGGGGTAGCAACAGAAAAGCGTTCATTCAAGCTACGAAGCGACAGCTTGAATTCTTTGAGGCTGAGACGAGTGTTTGATCTTTTTGACAACAATGGAGATGGCTTCATCACAGTGGATGAAATCAGACAGGCACTTGTGCTGCTGGGCCTTGAAGATGATCATATGAATAATAATAATAATAATAATACTAATAAAGAAGATGATCAGCTCGACTCCATTGTCACCTCCTTCATAAGGCCTGGTTACCAAGGTCTGGCCTTTGAAGATTTCCAGTCCCTTCATATCTCCTTAAACAACACCTTCTTCTTTGACTGCAACCTTGATCATCATCAACAACATCCTATTATTGATCAGGTGGAATCTGATCTCACTCAAGCTTTTAAGGTGTTTGATTCAGACGGAGATGGATACATCTCTGCGTCTGAGCTTCAGCAAGTACTCTCCAAATTGGGCTTTCCAGAGGCTCAACAAGAGCTCCATCACATCCAACTTATGATCTCCTCCTTCGACCACAACCACGATGGCCGTGTCGATTTCGGAGAATTCAAGCACATGATGTCCCGTACCCTTCTTCTTCCTGCTTCTTAG

mRNA sequence

ATGGGAGAAGTATCAAGTGATGAAATAATAATAATAATGGATAATAAGGCAGAGAAGTCGAAGAGGATGCTTGGGGTAGCAACAGAAAAGCGTTCATTCAAGCTACGAAGCGACAGCTTGAATTCTTTGAGGCTGAGACGAGTGTTTGATCTTTTTGACAACAATGGAGATGGCTTCATCACAGTGGATGAAATCAGACAGGCACTTGTGCTGCTGGGCCTTGAAGATGATCATATGAATAATAATAATAATAATAATACTAATAAAGAAGATGATCAGCTCGACTCCATTGTCACCTCCTTCATAAGGCCTGGTTACCAAGGTCTGGCCTTTGAAGATTTCCAGTCCCTTCATATCTCCTTAAACAACACCTTCTTCTTTGACTGCAACCTTGATCATCATCAACAACATCCTATTATTGATCAGGTGGAATCTGATCTCACTCAAGCTTTTAAGGTGTTTGATTCAGACGGAGATGGATACATCTCTGCGTCTGAGCTTCAGCAAGTACTCTCCAAATTGGGCTTTCCAGAGGCTCAACAAGAGCTCCATCACATCCAACTTATGATCTCCTCCTTCGACCACAACCACGATGGCCGTGTCGATTTCGGAGAATTCAAGCACATGATGTCCCGTACCCTTCTTCTTCCTGCTTCTTAG

Coding sequence (CDS)

ATGGGAGAAGTATCAAGTGATGAAATAATAATAATAATGGATAATAAGGCAGAGAAGTCGAAGAGGATGCTTGGGGTAGCAACAGAAAAGCGTTCATTCAAGCTACGAAGCGACAGCTTGAATTCTTTGAGGCTGAGACGAGTGTTTGATCTTTTTGACAACAATGGAGATGGCTTCATCACAGTGGATGAAATCAGACAGGCACTTGTGCTGCTGGGCCTTGAAGATGATCATATGAATAATAATAATAATAATAATACTAATAAAGAAGATGATCAGCTCGACTCCATTGTCACCTCCTTCATAAGGCCTGGTTACCAAGGTCTGGCCTTTGAAGATTTCCAGTCCCTTCATATCTCCTTAAACAACACCTTCTTCTTTGACTGCAACCTTGATCATCATCAACAACATCCTATTATTGATCAGGTGGAATCTGATCTCACTCAAGCTTTTAAGGTGTTTGATTCAGACGGAGATGGATACATCTCTGCGTCTGAGCTTCAGCAAGTACTCTCCAAATTGGGCTTTCCAGAGGCTCAACAAGAGCTCCATCACATCCAACTTATGATCTCCTCCTTCGACCACAACCACGATGGCCGTGTCGATTTCGGAGAATTCAAGCACATGATGTCCCGTACCCTTCTTCTTCCTGCTTCTTAG

Protein sequence

MGEVSSDEIIIIMDNKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMMSRTLLLPAS

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo19694	Spo19694	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo19694.1	Spo19694.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19694.1.CDS.1	Spo19694.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19694.1.exon.1	Spo19694.1.exon.1	exon

Homology

[Close]

BLAST of Spo19694.1 vs. NCBI nr
Match: gi|902189325|gb|KNA11350.1| (hypothetical protein SOVF_136020 [Spinacia oleracea])

HSP 1 Score: 396.7 bits (1018), Expect = 2.600e-107
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 1


		  
Query: 23  MLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNN 82
           MLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNN
Sbjct: 1   MLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNN 60

Query: 83  NNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQ 142
           NNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQ
Sbjct: 61  NNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQ 120

Query: 143 VESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVD 202
           VESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVD
Sbjct: 121 VESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVD 180

Query: 203 FGEFKHMMSRTLLLPAS 220
           FGEFKHMMSRTLLLPAS
Sbjct: 181 FGEFKHMMSRTLLLPAS 197

[Close]

BLAST of Spo19694.1 vs. NCBI nr
Match: gi|731344438|ref|XP_010683421.1| (PREDICTED: probable calcium-binding protein CML43 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 199.5 bits (506), Expect = 6.000e-48
Identity = 124/232 (53.45%), Postives = 148/232 (63.79%), Query Frame = 1


		  
Query: 2   GEVSSDEIIIIMDNKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFIT 61
           G V  ++II +  +   K +      +  RSFKLRS SLNSLRLRRVFDLFD NGDG I+
Sbjct: 14  GGVKEEKIIQVDIDVMGKQRLSRKKWSSSRSFKLRSGSLNSLRLRRVFDLFDKNGDGLIS 73

Query: 62  VDEIRQALVLLGLEDDHMNNNNNNN--TNKEDDQLDSIVTSFIRPGYQGL---AFEDFQS 121
           +DEIRQALVLLGL++D   +   N+  T+      + +     +  +  L    F D  S
Sbjct: 74  MDEIRQALVLLGLDEDGDEDEELNSIVTSFIKPGYEGLAFEDFQSLHNSLNNAIFYDIDS 133

Query: 122 LHISLNNTFFFDCNLDHHQQHPIID----------QVESDLTQAFKVFDSDGDGYISASE 181
              +  +    D +    QQH              QVESDLT+AFKVFDS+GDGYISA+E
Sbjct: 134 SSATSLDPHDEDHDPQQQQQHQQQQHQHQHQHQHQQVESDLTEAFKVFDSNGDGYISATE 193

Query: 182 LQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMMSRTLLLPA 219
           LQQVLSKLGFPEAQQELHHIQLMISSFD NHDGRVDF EFKHMMSR LL P+
Sbjct: 194 LQQVLSKLGFPEAQQELHHIQLMISSFDQNHDGRVDFFEFKHMMSRILLFPS 245

[Close]

BLAST of Spo19694.1 vs. NCBI nr
Match: gi|545670760|gb|AGW45458.1| (calcium binding protein [Reaumuria trigyna])

HSP 1 Score: 199.5 bits (506), Expect = 6.000e-48
Identity = 117/219 (53.42%), Postives = 141/219 (64.38%), Query Frame = 1


		  
Query: 18  EKSKRMLGVATEKRS-FKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLED 77
           +K+  +  +++ K S F+LR  SLN+LRLRRVFDLFD NGDG IT++EI QALVLLGLE 
Sbjct: 8   DKAAALRSLSSRKSSSFRLRCSSLNALRLRRVFDLFDKNGDGLITIEEISQALVLLGLE- 67

Query: 78  DHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDC------- 137
                     T+  +  LDSI+ S+I+PG  GL F+DF+SLH SLN TFF DC       
Sbjct: 68  ----------TDPSESALDSIIKSYIKPGNTGLTFDDFESLHKSLNETFF-DCEDDIDLC 127

Query: 138 --------NLDHHQQHPII-----DQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGF 197
                   N++   +         DQVESDLT+AFKVFD DGDGYISA ELQ VL KLG 
Sbjct: 128 CGEVQVSNNVNEENKGAYCIEDDEDQVESDLTEAFKVFDVDGDGYISAKELQAVLDKLGM 187

Query: 198 PEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMMSRTLL 216
           PEA+ EL  IQLMI+S D N DG VDF EFK MM   L+
Sbjct: 188 PEARDELDRIQLMINSVDRNQDGLVDFFEFKDMMRSVLV 214

[Close]

BLAST of Spo19694.1 vs. NCBI nr
Match: gi|870854727|gb|KMT06475.1| (hypothetical protein BVRB_7g156540 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 199.5 bits (506), Expect = 6.000e-48
Identity = 124/232 (53.45%), Postives = 148/232 (63.79%), Query Frame = 1


		  
Query: 2   GEVSSDEIIIIMDNKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFIT 61
           G V  ++II +  +   K +      +  RSFKLRS SLNSLRLRRVFDLFD NGDG I+
Sbjct: 7   GGVKEEKIIQVDIDVMGKQRLSRKKWSSSRSFKLRSGSLNSLRLRRVFDLFDKNGDGLIS 66

Query: 62  VDEIRQALVLLGLEDDHMNNNNNNN--TNKEDDQLDSIVTSFIRPGYQGL---AFEDFQS 121
           +DEIRQALVLLGL++D   +   N+  T+      + +     +  +  L    F D  S
Sbjct: 67  MDEIRQALVLLGLDEDGDEDEELNSIVTSFIKPGYEGLAFEDFQSLHNSLNNAIFYDIDS 126

Query: 122 LHISLNNTFFFDCNLDHHQQHPIID----------QVESDLTQAFKVFDSDGDGYISASE 181
              +  +    D +    QQH              QVESDLT+AFKVFDS+GDGYISA+E
Sbjct: 127 SSATSLDPHDEDHDPQQQQQHQQQQHQHQHQHQHQQVESDLTEAFKVFDSNGDGYISATE 186

Query: 182 LQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMMSRTLLLPA 219
           LQQVLSKLGFPEAQQELHHIQLMISSFD NHDGRVDF EFKHMMSR LL P+
Sbjct: 187 LQQVLSKLGFPEAQQELHHIQLMISSFDQNHDGRVDFFEFKHMMSRILLFPS 238

[Close]

BLAST of Spo19694.1 vs. NCBI nr
Match: gi|848862123|ref|XP_012831857.1| (PREDICTED: calcium-binding protein CAST [Erythranthe guttata])

HSP 1 Score: 194.5 bits (493), Expect = 1.900e-46
Identity = 110/207 (53.14%), Postives = 142/207 (68.60%), Query Frame = 1


		  
Query: 14  DNKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLG 73
           D   ++S   LG A+   SF+LRS SLN++RLRR+FDLFD N D  ITVDE+ +AL LLG
Sbjct: 8   DEMVKRSSISLGGASN--SFRLRSPSLNAVRLRRIFDLFDKNKDSVITVDELGEALSLLG 67

Query: 74  LEDDHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFF-FDCNLD 133
           L+ D               QL+S+V ++++ G QGL++EDF+SLH SLN+ FF  D N +
Sbjct: 68  LQTDI-------------SQLESMVGAYVKQGNQGLSYEDFESLHRSLNDVFFGSDVNNE 127

Query: 134 HHQQHPIIDQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISS 193
               H   D  ESDL++AFKVFD DGDGYISA ELQ VL KLG  +  +EL  +++MISS
Sbjct: 128 IIGDHRSDDDDESDLSEAFKVFDMDGDGYISARELQSVLGKLGLHDEGRELDRVEMMISS 187

Query: 194 FDHNHDGRVDFGEFKHMMSRTLLLPAS 220
            D NHDGRVDF EFKHMM RT+++P+S
Sbjct: 188 VDQNHDGRVDFFEFKHMM-RTVIVPSS 198

[Close]

BLAST of Spo19694.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QVR8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_136020 PE=4 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 1.800e-107
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 1


		  
Query: 23  MLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNN 82
           MLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNN
Sbjct: 1   MLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNN 60

Query: 83  NNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQ 142
           NNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQ
Sbjct: 61  NNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQ 120

Query: 143 VESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVD 202
           VESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVD
Sbjct: 121 VESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVD 180

Query: 203 FGEFKHMMSRTLLLPAS 220
           FGEFKHMMSRTLLLPAS
Sbjct: 181 FGEFKHMMSRTLLLPAS 197

[Close]

BLAST of Spo19694.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BY95_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g156540 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.200e-48
Identity = 124/232 (53.45%), Postives = 148/232 (63.79%), Query Frame = 1


		  
Query: 2   GEVSSDEIIIIMDNKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFIT 61
           G V  ++II +  +   K +      +  RSFKLRS SLNSLRLRRVFDLFD NGDG I+
Sbjct: 7   GGVKEEKIIQVDIDVMGKQRLSRKKWSSSRSFKLRSGSLNSLRLRRVFDLFDKNGDGLIS 66

Query: 62  VDEIRQALVLLGLEDDHMNNNNNNN--TNKEDDQLDSIVTSFIRPGYQGL---AFEDFQS 121
           +DEIRQALVLLGL++D   +   N+  T+      + +     +  +  L    F D  S
Sbjct: 67  MDEIRQALVLLGLDEDGDEDEELNSIVTSFIKPGYEGLAFEDFQSLHNSLNNAIFYDIDS 126

Query: 122 LHISLNNTFFFDCNLDHHQQHPIID----------QVESDLTQAFKVFDSDGDGYISASE 181
              +  +    D +    QQH              QVESDLT+AFKVFDS+GDGYISA+E
Sbjct: 127 SSATSLDPHDEDHDPQQQQQHQQQQHQHQHQHQHQQVESDLTEAFKVFDSNGDGYISATE 186

Query: 182 LQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMMSRTLLLPA 219
           LQQVLSKLGFPEAQQELHHIQLMISSFD NHDGRVDF EFKHMMSR LL P+
Sbjct: 187 LQQVLSKLGFPEAQQELHHIQLMISSFDQNHDGRVDFFEFKHMMSRILLFPS 238

[Close]

BLAST of Spo19694.1 vs. UniProtKB/TrEMBL
Match: A0A075C736_9CARY (Calcium binding protein OS=Reaumuria trigyna PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.200e-48
Identity = 117/219 (53.42%), Postives = 141/219 (64.38%), Query Frame = 1


		  
Query: 18  EKSKRMLGVATEKRS-FKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLED 77
           +K+  +  +++ K S F+LR  SLN+LRLRRVFDLFD NGDG IT++EI QALVLLGLE 
Sbjct: 8   DKAAALRSLSSRKSSSFRLRCSSLNALRLRRVFDLFDKNGDGLITIEEISQALVLLGLE- 67

Query: 78  DHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDC------- 137
                     T+  +  LDSI+ S+I+PG  GL F+DF+SLH SLN TFF DC       
Sbjct: 68  ----------TDPSESALDSIIKSYIKPGNTGLTFDDFESLHKSLNETFF-DCEDDIDLC 127

Query: 138 --------NLDHHQQHPII-----DQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGF 197
                   N++   +         DQVESDLT+AFKVFD DGDGYISA ELQ VL KLG 
Sbjct: 128 CGEVQVSNNVNEENKGAYCIEDDEDQVESDLTEAFKVFDVDGDGYISAKELQAVLDKLGM 187

Query: 198 PEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMMSRTLL 216
           PEA+ EL  IQLMI+S D N DG VDF EFK MM   L+
Sbjct: 188 PEARDELDRIQLMINSVDRNQDGLVDFFEFKDMMRSVLV 214

[Close]

BLAST of Spo19694.1 vs. UniProtKB/TrEMBL
Match: A0A022RNK4_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a014170mg PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.300e-46
Identity = 110/207 (53.14%), Postives = 142/207 (68.60%), Query Frame = 1


		  
Query: 14  DNKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLG 73
           D   ++S   LG A+   SF+LRS SLN++RLRR+FDLFD N D  ITVDE+ +AL LLG
Sbjct: 8   DEMVKRSSISLGGASN--SFRLRSPSLNAVRLRRIFDLFDKNKDSVITVDELGEALSLLG 67

Query: 74  LEDDHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFF-FDCNLD 133
           L+ D               QL+S+V ++++ G QGL++EDF+SLH SLN+ FF  D N +
Sbjct: 68  LQTDI-------------SQLESMVGAYVKQGNQGLSYEDFESLHRSLNDVFFGSDVNNE 127

Query: 134 HHQQHPIIDQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISS 193
               H   D  ESDL++AFKVFD DGDGYISA ELQ VL KLG  +  +EL  +++MISS
Sbjct: 128 IIGDHRSDDDDESDLSEAFKVFDMDGDGYISARELQSVLGKLGLHDEGRELDRVEMMISS 187

Query: 194 FDHNHDGRVDFGEFKHMMSRTLLLPAS 220
            D NHDGRVDF EFKHMM RT+++P+S
Sbjct: 188 VDQNHDGRVDFFEFKHMM-RTVIVPSS 198

[Close]

BLAST of Spo19694.1 vs. UniProtKB/TrEMBL
Match: A0A164SY50_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023958 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 5.100e-46
Identity = 102/197 (51.78%), Postives = 137/197 (69.54%), Query Frame = 1


		  
Query: 21  KRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMN 80
           KR L  +++  SFKLRS SLNS+RLRR+FD+FD + DG +T DE+ QAL +LGL+ D   
Sbjct: 10  KRSLSSSSKSNSFKLRSPSLNSVRLRRIFDMFDKSSDGMLTADELSQALSILGLDAD--- 69

Query: 81  NNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPII 140
                       ++DS+++S+++PG QGL FEDF++LH SLNN FF     D  +  P+ 
Sbjct: 70  ----------PSEIDSMISSYVKPGNQGLTFEDFEALHQSLNNAFF----ADGQEDAPVS 129

Query: 141 DQ--VESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHD 200
           ++   ESDL++AFKVFD DGDG+ISA ELQ VL KLGFPE+ +EL  +++MISS D N D
Sbjct: 130 EEESQESDLSEAFKVFDEDGDGFISAKELQVVLGKLGFPES-RELARVEMMISSVDRNKD 188

Query: 201 GRVDFGEFKHMMSRTLL 216
           GRVDF EFK MM   ++
Sbjct: 190 GRVDFFEFKDMMRNVMV 188

[Close]

BLAST of Spo19694.1 vs. ExPASy Swiss-Prot
Match: CML42_ARATH (Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.000e-43
Identity = 106/207 (51.21%), Postives = 135/207 (65.22%), Query Frame = 1


		  
Query: 15  NKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGL 74
           N  EK K    VA +  SF+LRS SLN+LRL+R+FDLFD NGDGFITV+E+ QAL  LGL
Sbjct: 4   NNNEKKK----VARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGL 63

Query: 75  EDDHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFD-CNLDH 134
                        N +   L S V S+I+PG  GL F+DF SLH +L+++FF   C    
Sbjct: 64  -------------NADLSDLKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGE 123

Query: 135 HQQHP-IIDQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISS 194
           ++  P    + ESDL +AFKVFD +GDG+ISA ELQ VL KLG PE   E+  ++ MI S
Sbjct: 124 NEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLGLPEG-GEMERVEKMIVS 183

Query: 195 FDHNHDGRVDFGEFKHMMSRTLLLPAS 220
            D N DGRVDF EFK+MM RT+++P+S
Sbjct: 184 VDRNQDGRVDFFEFKNMM-RTVVIPSS 191

[Close]

BLAST of Spo19694.1 vs. ExPASy Swiss-Prot
Match: ALLB3_BETPN (Calcium-binding allergen Bet v 3 OS=Betula pendula GN=BETVIII PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.100e-41
Identity = 99/189 (52.38%), Postives = 120/189 (63.49%), Query Frame = 1


		  
Query: 32  SFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNNNNNNTNKED 91
           SF+LRS+SLN+LRLRR+FDLFD N DG ITVDE+ +AL LLGLE D              
Sbjct: 28  SFRLRSESLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDL------------- 87

Query: 92  DQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFF-----DCNLDHHQQHPIIDQVESD 151
            +L+S V SF R G  GL FEDF SLH SLN+++F      + + +   +  I+ Q E+D
Sbjct: 88  SELESTVKSFTREGNIGLQFEDFISLHQSLNDSYFAYGGEDEDDNEEDMRKSILSQEEAD 147

Query: 152 LTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEF 211
               FKVFD DGDGYISA ELQ VL KLGF E   E+  ++ MI S D N DGRVDF EF
Sbjct: 148 SFGGFKVFDEDGDGYISARELQMVLGKLGFSEG-SEIDRVEKMIVSVDSNRDGRVDFFEF 202

Query: 212 KHMMSRTLL 216
           K MM   L+
Sbjct: 208 KDMMRSVLV 202

[Close]

BLAST of Spo19694.1 vs. ExPASy Swiss-Prot
Match: CAST_SOLTU (Calcium-binding protein CAST OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.600e-40
Identity = 95/187 (50.80%), Postives = 125/187 (66.84%), Query Frame = 1


		  
Query: 32  SFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNNNNNNTNKED 91
           SF+LRS SLNS+RLRR+FD+FD N D  I+V+E+ QAL LLGL+ D              
Sbjct: 28  SFRLRSPSLNSIRLRRIFDVFDRNHDCLISVEELSQALNLLGLDADL------------- 87

Query: 92  DQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQVESDLTQAF 151
            +++S+V   I+P   GL FEDF++LH SLN+ FF     D    +P   Q ESDL +AF
Sbjct: 88  SEIESMVKLHIKPENTGLRFEDFETLHRSLNDVFFGSKCEDKLGLNPDPAQDESDLKEAF 147

Query: 152 KVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMMS 211
            VFD +GDG+ISA ELQ VL KLG PE   E+  +++MISS + +HDGRVDF EFK MM 
Sbjct: 148 DVFDENGDGFISAKELQVVLEKLGLPEG-SEIDRVEMMISSVEQDHDGRVDFFEFKDMM- 199

Query: 212 RTLLLPA 219
           RT+++P+
Sbjct: 208 RTVIVPS 199

[Close]

BLAST of Spo19694.1 vs. ExPASy Swiss-Prot
Match: CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 6.000e-40
Identity = 101/206 (49.03%), Postives = 129/206 (62.62%), Query Frame = 1


		  
Query: 13  MDNKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLL 72
           M+   EK K    ++ +  SF+LRS SLN+LRL RVFDLFD N DGFITV+E+ QAL  L
Sbjct: 1   MEINNEKKK----LSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRL 60

Query: 73  GLEDDHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLD 132
           GL+ D                L S V SFI+P   GL F+DF +LH +L+ +FF      
Sbjct: 61  GLDADF-------------SDLKSTVDSFIKPDKTGLRFDDFAALHKTLDESFF------ 120

Query: 133 HHQQHPIIDQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISS 192
             +        ESDL +AF VFD DGDG+ISA ELQ+VL KLG PEA  E+  ++ MI S
Sbjct: 121 GGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEA-GEIEQVEKMIVS 180

Query: 193 FDHNHDGRVDFGEFKHMMSRTLLLPA 219
            D NHDGRVDF EFK+MM +T+++P+
Sbjct: 181 VDSNHDGRVDFFEFKNMM-QTVVVPS 181

[Close]

BLAST of Spo19694.1 vs. ExPASy Swiss-Prot
Match: CML32_ORYSJ (Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica GN=CML32 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.000e-36
Identity = 94/193 (48.70%), Postives = 117/193 (60.62%), Query Frame = 1


		  
Query: 20  SKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHM 79
           +K  L   T   SF+LR+ SLN++RLRRVFDLFD NGDG ITVDE+ QAL  LGL  D  
Sbjct: 10  AKPSLAKKTASASFRLRNGSLNAVRLRRVFDLFDRNGDGEITVDELAQALDALGLVADR- 69

Query: 80  NNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFF--FDCNLDHHQQH 139
                       D L + V++++  G  GL FEDF +LH +L +  F   D      +  
Sbjct: 70  ------------DGLAATVSAYVPEGAAGLRFEDFDALHRALGDALFGSLDGAAAAGEPG 129

Query: 140 PIIDQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNH 199
                 E ++ +AFKVFD DGDG+ISASELQ+VL KLG PEA   L  ++ MI + D N 
Sbjct: 130 GGGGDEEEEMREAFKVFDVDGDGFISASELQEVLKKLGLPEA-GSLATVREMICNVDRNS 188

Query: 200 DGRVDFGEFKHMM 211
           DGRVDFGEFK MM
Sbjct: 190 DGRVDFGEFKSMM 188

[Close]

BLAST of Spo19694.1 vs. TAIR (Arabidopsis)
Match: AT4G20780.1 (calmodulin like 42)

HSP 1 Score: 177.2 bits (448), Expect = 1.100e-44
Identity = 106/207 (51.21%), Postives = 135/207 (65.22%), Query Frame = 1


		  
Query: 15  NKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGL 74
           N  EK K    VA +  SF+LRS SLN+LRL+R+FDLFD NGDGFITV+E+ QAL  LGL
Sbjct: 4   NNNEKKK----VARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGL 63

Query: 75  EDDHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFD-CNLDH 134
                        N +   L S V S+I+PG  GL F+DF SLH +L+++FF   C    
Sbjct: 64  -------------NADLSDLKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGE 123

Query: 135 HQQHP-IIDQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISS 194
           ++  P    + ESDL +AFKVFD +GDG+ISA ELQ VL KLG PE   E+  ++ MI S
Sbjct: 124 NEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLGLPEG-GEMERVEKMIVS 183

Query: 195 FDHNHDGRVDFGEFKHMMSRTLLLPAS 220
            D N DGRVDF EFK+MM RT+++P+S
Sbjct: 184 VDRNQDGRVDFFEFKNMM-RTVVIPSS 191

[Close]

BLAST of Spo19694.1 vs. TAIR (Arabidopsis)
Match: AT5G44460.1 (calmodulin like 43)

HSP 1 Score: 165.6 bits (418), Expect = 3.400e-41
Identity = 101/206 (49.03%), Postives = 129/206 (62.62%), Query Frame = 1


		  
Query: 13  MDNKAEKSKRMLGVATEKRSFKLRSDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLL 72
           M+   EK K    ++ +  SF+LRS SLN+LRL RVFDLFD N DGFITV+E+ QAL  L
Sbjct: 1   MEINNEKKK----LSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRL 60

Query: 73  GLEDDHMNNNNNNNTNKEDDQLDSIVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLD 132
           GL+ D                L S V SFI+P   GL F+DF +LH +L+ +FF      
Sbjct: 61  GLDADF-------------SDLKSTVDSFIKPDKTGLRFDDFAALHKTLDESFF------ 120

Query: 133 HHQQHPIIDQVESDLTQAFKVFDSDGDGYISASELQQVLSKLGFPEAQQELHHIQLMISS 192
             +        ESDL +AF VFD DGDG+ISA ELQ+VL KLG PEA  E+  ++ MI S
Sbjct: 121 GGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEA-GEIEQVEKMIVS 180

Query: 193 FDHNHDGRVDFGEFKHMMSRTLLLPA 219
            D NHDGRVDF EFK+MM +T+++P+
Sbjct: 181 VDSNHDGRVDFFEFKNMM-QTVVVPS 181

[Close]

BLAST of Spo19694.1 vs. TAIR (Arabidopsis)
Match: AT1G21550.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 80.5 bits (197), Expect = 1.400e-15
Identity = 62/169 (36.69%), Postives = 83/169 (49.11%), Query Frame = 1


		  
Query: 45  LRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNNNNNNTNKEDDQLDSIVTSFIRP 104
           LRR+F   D N DG +T+DE+   L  LG  +               D+L+ IV      
Sbjct: 11  LRRMFKTLDKNQDGLVTLDELLWILDKLGWAEH------------TPDELELIV------ 70

Query: 105 GYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQVESD---LTQAFKVFDSDGDGY 164
           G Q L  ++F          F++D  LD       ID V  +   + +AF VFD +GDGY
Sbjct: 71  GKQSLDLDEFLR--------FYYDAVLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGY 130

Query: 165 ISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMM 211
           ISA EL+ VL +LGF E + +      MI   D N DG VDF EFK+M+
Sbjct: 131 ISAEELRDVLERLGF-EEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMI 152

[Close]

BLAST of Spo19694.1 vs. TAIR (Arabidopsis)
Match: AT2G43290.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 80.5 bits (197), Expect = 1.400e-15
Identity = 54/166 (32.53%), Postives = 83/166 (50.00%), Query Frame = 1


		  
Query: 45  LRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNNNNNNTNKEDDQLDSIVTSFIRP 104
           L+RVF +FD NGDG IT +E+  +L  LG+                D  L  ++      
Sbjct: 66  LKRVFQMFDKNGDGRITKEELNDSLENLGIYIP-------------DKDLTQMIHKIDAN 125

Query: 105 GYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQVESDLTQAFKVFDSDGDGYISA 164
           G   +  ++F+SL+ S+ +        +HH      +  E D+  AF VFD DGDG+I+ 
Sbjct: 126 GDGCVDIDEFESLYSSIVD--------EHHNDG---ETEEEDMKDAFNVFDQDGDGFITV 185

Query: 165 SELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMM 211
            EL+ V++ LG  +  + L   + MI   D + DGRV++ EF  MM
Sbjct: 186 EELKSVMASLGLKQG-KTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

[Close]

BLAST of Spo19694.1 vs. TAIR (Arabidopsis)
Match: AT3G59440.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 79.0 bits (193), Expect = 4.200e-15
Identity = 53/174 (30.46%), Postives = 84/174 (48.28%), Query Frame = 1


		  
Query: 37  SDSLNSLRLRRVFDLFDNNGDGFITVDEIRQALVLLGLEDDHMNNNNNNNTNKEDDQLDS 96
           S++ + + L+RVF +FD NGDG IT +E+  +L  LG+                D  L  
Sbjct: 44  SETESPVDLKRVFQMFDKNGDGRITKEELNDSLENLGIF-------------MPDKDLIQ 103

Query: 97  IVTSFIRPGYQGLAFEDFQSLHISLNNTFFFDCNLDHHQQHPIIDQVESDLTQAFKVFDS 156
           ++      G   +   +F+SL+ S+                 + ++ E D+  AF VFD 
Sbjct: 104 MIQKMDANGDGCVDINEFESLYGSI-----------------VEEKEEGDMRDAFNVFDQ 163

Query: 157 DGDGYISASELQQVLSKLGFPEAQQELHHIQLMISSFDHNHDGRVDFGEFKHMM 211
           DGDG+I+  EL  V++ LG  +  + L   + MI   D + DGRV++ EF  MM
Sbjct: 164 DGDGFITVEELNSVMTSLGLKQG-KTLECCKEMIMQVDEDGDGRVNYKEFLQMM 186

The following BLAST results are available for this feature:

BLAST of Spo19694.1 vs. NCBI nr
BLAST of Spo19694.1 vs. UniProtKB/TrEMBL
BLAST of Spo19694.1 vs. ExPASy Swiss-Prot
BLAST of Spo19694.1 vs. TAIR (Arabidopsis)

BLAST of Spo19694.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902189325\|gb\|KNA11350.1\|	2.6e-107	100.	hypothetical protein SOVF_1360...	[more]
gi\|731344438\|ref\|XP_010683421.1\|	6.0e-48	53.4	PREDICTED: probable calcium-bi...	[more]
gi\|545670760\|gb\|AGW45458.1\|	6.0e-48	53.4	calcium binding protein [Reaum...	[more]
gi\|870854727\|gb\|KMT06475.1\|	6.0e-48	53.4	hypothetical protein BVRB_7g15...	[more]
gi\|848862123\|ref\|XP_012831857.1\|	1.9e-46	53.1	PREDICTED: calcium-binding pro...	[more]

BLAST of Spo19694.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QVR8_SPIOL	1.8e-107	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8BY95_BETVU	4.2e-48	53.4	Uncharacterized protein OS=Bet...	[more]
A0A075C736_9CARY	4.2e-48	53.4	Calcium binding protein OS=Rea...	[more]
A0A022RNK4_ERYGU	1.3e-46	53.1	Uncharacterized protein OS=Ery...	[more]
A0A164SY50_DAUCA	5.1e-46	51.7	Uncharacterized protein OS=Dau...	[more]

BLAST of Spo19694.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
CML42_ARATH	2.0e-43	51.2	Calcium-binding protein CML42 ...	[more]
ALLB3_BETPN	7.1e-41	52.3	Calcium-binding allergen Bet v...	[more]
CAST_SOLTU	4.6e-40	50.8	Calcium-binding protein CAST O...	[more]
CML43_ARATH	6.0e-40	49.0	Probable calcium-binding prote...	[more]
CML32_ORYSJ	4.0e-36	48.7	Probable calcium-binding prote...	[more]

BLAST of Spo19694.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G20780.1	1.1e-44	51.2	calmodulin like 42	[more]
AT5G44460.1	3.4e-41	49.0	calmodulin like 43	[more]
AT1G21550.1	1.4e-15	36.6	Calcium-binding EF-hand family...	[more]
AT2G43290.1	1.4e-15	32.5	Calcium-binding EF-hand family...	[more]
AT3G59440.1	4.2e-15	30.4	Calcium-binding EF-hand family...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 44..73 score: 9.
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 145..210 score: 2.4
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 146..174 score: 3.2E-6coord: 185..213 score: 2.1E-4coord: 44..72 score: 1.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 40..75 score: 15.294coord: 181..216 score: 11.947coord: 142..177 score: 15
IPR011992	EF-hand domain pair	GENE3D	1.10.238.10		coord: 68..115 score: 6.2E-4coord: 145..212 score: 2.4E-20coord: 2..67 score: 7.
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 44..212 score: 3.53
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 155..167 score: -coord: 53..65 score: -coord: 194..206 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..210 score: 6.1
None	No IPR available	PANTHER	PTHR10891:SF589	CALCIUM-BINDING PROTEIN CML42-RELATED	coord: 1..210 score: 6.1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010091	trichome branching
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0005515	protein binding