Spo14687.1 (mRNA) | SpinachBase

Overview


Name	Spo14687.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	rRNA intron-encoded homing endonuclease
Location	SpoScf_05643 : 1970 .. 2810 (-)
Sequence length	507

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGTGACGGGTGACGGAGAATTAAGGTTCGATTCCGGTGAGGGTGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCATTGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

mRNA sequence

ATGGTGGTGACGGGTGACGGAGAATTAAGGTTCGATTCCGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Coding sequence (CDS)

ATGGTGGTGACGGGTGACGGAGAATTAAGGTTCGATTCCGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Protein sequence

MVVTGDGELRFDSVKKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo14687	Spo14687	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo14687.1	Spo14687.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo14687.1.CDS.3	Spo14687.1.CDS.3	CDS
Spo14687.1.CDS.2	Spo14687.1.CDS.2	CDS
Spo14687.1.CDS.1	Spo14687.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo14687.1.exon.3	Spo14687.1.exon.3	exon
Spo14687.1.exon.2	Spo14687.1.exon.2	exon
Spo14687.1.exon.1	Spo14687.1.exon.1	exon

Homology

[Close]

BLAST of Spo14687.1 vs. NCBI nr
Match: gi|703137890|ref|XP_010106597.1| (hypothetical protein L484_002552 [Morus notabilis])

HSP 1 Score: 264.6 bits (675), Expect = 1.200e-67
Identity = 131/138 (94.93%), Postives = 132/138 (95.65%), Query Frame = 1


		  
Query: 31  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 90
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 91  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGGSMVAR 150
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSKFLGSGGSMVAR
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGSMVAR 174

Query: 151 LKLKGIDGRAPPGVEPAA 169
           LKLKGIDGRAPPGVEPAA
Sbjct: 175 LKLKGIDGRAPPGVEPAA 192

[Close]

BLAST of Spo14687.1 vs. NCBI nr
Match: gi|823127874|ref|XP_012440607.1| (PREDICTED: uncharacterized protein LOC105765883 [Gossypium raimondii])

HSP 1 Score: 226.5 bits (576), Expect = 3.500e-56
Identity = 117/153 (76.47%), Postives = 123/153 (80.39%), Query Frame = 1


		  
Query: 13  SVKKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALY 72
           +VKKLVVG  GG+ GPP G HR +R + RRCAPGLNWPGR+S  VTLKKLECSKQAYA  
Sbjct: 39  TVKKLVVGLRGGSAGPPHGEHRSARPYCRRCAPGLNWPGRSSDAVTLKKLECSKQAYA-- 98

Query: 73  TLAWDNIIGFRRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPP 132
                           +FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPP
Sbjct: 99  ----------------SFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPP 158

Query: 133 APYEKSKFLGSGGSMVARLKLKGIDGRAPPGVE 166
           APYEKSK LGSGGSMVARLKLKGIDGRAPPGVE
Sbjct: 159 APYEKSKSLGSGGSMVARLKLKGIDGRAPPGVE 173

[Close]

BLAST of Spo14687.1 vs. NCBI nr
Match: gi|694380941|ref|XP_009366572.1| (PREDICTED: uncharacterized protein LOC103956333 [Pyrus x bretschneideri])

HSP 1 Score: 224.6 bits (571), Expect = 1.300e-55
Identity = 118/142 (83.10%), Postives = 121/142 (85.21%), Query Frame = 1


		  
Query: 31  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRRT 90
           GVHR +R F RR APGLNWPGRASG VTLKKLECSKQ +    LA+      N     RT
Sbjct: 228 GVHRSARPFCRRYAPGLNWPGRASGAVTLKKLECSKQDFGPILLAFGIGVMINRDSRGRT 287

Query: 91  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG 150
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG
Sbjct: 288 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG 347

Query: 151 SMVARLKLKGIDGRAPPGVEPA 168
           SMVARLKLKGIDGRAPPGVEPA
Sbjct: 348 SMVARLKLKGIDGRAPPGVEPA 369

[Close]

BLAST of Spo14687.1 vs. NCBI nr
Match: gi|922327155|ref|XP_013442970.1| (hypothetical protein MTR_0055s0130 [Medicago truncatula])

HSP 1 Score: 218.4 bits (555), Expect = 9.600e-54
Identity = 116/143 (81.12%), Postives = 119/143 (83.22%), Query Frame = 1


		  
Query: 30  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRR 89
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ      LA+      N     R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 90  TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSG 149
           TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK LGSG
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 604

Query: 150 GSMVARLKLKGIDGRAPPGVEPA 168
           GSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 GSMVARLKLKGIDGRAPPGVEPA 627

[Close]

BLAST of Spo14687.1 vs. NCBI nr
Match: gi|947068904|gb|KRH17795.1| (hypothetical protein GLYMA_13G016700 [Glycine max])

HSP 1 Score: 215.7 bits (548), Expect = 6.200e-53
Identity = 116/143 (81.12%), Postives = 118/143 (82.52%), Query Frame = 1


		  
Query: 31  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRRT 90
           GVHR +R F RRCAP LNWPGRASG VTLKKLECSKQ      LA+      N     RT
Sbjct: 128 GVHRSARPFCRRCAPVLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGRT 187

Query: 91  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG 150
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK LGSGG
Sbjct: 188 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGG 247

Query: 151 SMVARLKLKGIDGRAPPGVEPAA 169
           SMVARLKLKGIDGRAPPGVEPAA
Sbjct: 248 SMVARLKLKGIDGRAPPGVEPAA 270

[Close]

BLAST of Spo14687.1 vs. UniProtKB/TrEMBL
Match: M1DUP9_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400044276 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 9.600e-69
Identity = 133/141 (94.33%), Postives = 133/141 (94.33%), Query Frame = 1


		  
Query: 28  PPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRTTA 87
           P   VHR SR F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRRTTA
Sbjct: 45  PCICVHRSSRPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTA 104

Query: 88  KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGGSM 147
           KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYEKSKFLGSGGSM
Sbjct: 105 KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLGSGGSM 164

Query: 148 VARLKLKGIDGRAPPGVEPAA 169
           VARLKLKGIDGRAPPGVEPAA
Sbjct: 165 VARLKLKGIDGRAPPGVEPAA 185

[Close]

BLAST of Spo14687.1 vs. UniProtKB/TrEMBL
Match: W9SJ11_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_002552 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 8.100e-68
Identity = 131/138 (94.93%), Postives = 132/138 (95.65%), Query Frame = 1


		  
Query: 31  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 90
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 91  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGGSMVAR 150
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSKFLGSGGSMVAR
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGSMVAR 174

Query: 151 LKLKGIDGRAPPGVEPAA 169
           LKLKGIDGRAPPGVEPAA
Sbjct: 175 LKLKGIDGRAPPGVEPAA 192

[Close]

BLAST of Spo14687.1 vs. UniProtKB/TrEMBL
Match: M1DB49_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400035737 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 7.100e-64
Identity = 124/130 (95.38%), Postives = 126/130 (96.92%), Query Frame = 1


		  
Query: 39  FRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAFAKDVFINQ 98
           F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAFAKDVFINQ
Sbjct: 5   FCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAFAKDVFINQ 64

Query: 99  ERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGGSMVARLKLKGIDG 158
           ERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYEKSKFLGSGGS+VARLKLKGIDG
Sbjct: 65  ERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLGSGGSIVARLKLKGIDG 124

Query: 159 RAPPGVEPAA 169
           +APPGVE AA
Sbjct: 125 KAPPGVELAA 134

[Close]

BLAST of Spo14687.1 vs. UniProtKB/TrEMBL
Match: A0A0D2ZQ67_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 9.000e-59
Identity = 120/142 (84.51%), Postives = 124/142 (87.32%), Query Frame = 1


		  
Query: 31  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRTTA--- 90
           G+HR +  F R+ APGLNWPGRASG VTLKKLECSKQAY LYTLAWDNIIGF    A   
Sbjct: 189 GMHRSACPFCRQYAPGLNWPGRASGAVTLKKLECSKQAYTLYTLAWDNIIGFLSYCAGLQ 248

Query: 91  -KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGGS 150
            + FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSKFLGSGGS
Sbjct: 249 DRTFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGS 308

Query: 151 MVARLKLKGIDGRAPPGVEPAA 169
           MVARLKLKGIDGRAPPGVEPAA
Sbjct: 309 MVARLKLKGIDGRAPPGVEPAA 330

[Close]

BLAST of Spo14687.1 vs. UniProtKB/TrEMBL
Match: A0A072TTW7_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_0055s0130 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 6.700e-54
Identity = 116/143 (81.12%), Postives = 119/143 (83.22%), Query Frame = 1


		  
Query: 30  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRR 89
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ      LA+      N     R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 90  TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSG 149
           TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK LGSG
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 604

Query: 150 GSMVARLKLKGIDGRAPPGVEPA 168
           GSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 GSMVARLKLKGIDGRAPPGVEPA 627

The following BLAST results are available for this feature:

BLAST of Spo14687.1 vs. NCBI nr
BLAST of Spo14687.1 vs. UniProtKB/TrEMBL
BLAST of Spo14687.1 vs. ExPASy Swiss-Prot
BLAST of Spo14687.1 vs. TAIR (Arabidopsis)

BLAST of Spo14687.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|703137890\|ref\|XP_010106597.1\|	1.2e-67	94.9	hypothetical protein L484_0025...	[more]
gi\|823127874\|ref\|XP_012440607.1\|	3.5e-56	76.4	PREDICTED: uncharacterized pro...	[more]
gi\|694380941\|ref\|XP_009366572.1\|	1.3e-55	83.1	PREDICTED: uncharacterized pro...	[more]
gi\|922327155\|ref\|XP_013442970.1\|	9.6e-54	81.1	hypothetical protein MTR_0055s...	[more]
gi\|947068904\|gb\|KRH17795.1\|	6.2e-53	81.1	hypothetical protein GLYMA_13G...	[more]

BLAST of Spo14687.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
M1DUP9_SOLTU	9.6e-69	94.3	Uncharacterized protein OS=Sol...	[more]
W9SJ11_9ROSA	8.1e-68	94.9	Uncharacterized protein OS=Mor...	[more]
M1DB49_SOLTU	7.1e-64	95.3	Uncharacterized protein OS=Sol...	[more]
A0A0D2ZQ67_BRAOL	9.0e-59	84.5	Uncharacterized protein OS=Bra...	[more]
A0A072TTW7_MEDTR	6.7e-54	81.1	Uncharacterized protein OS=Med...	[more]

BLAST of Spo14687.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo14687.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34410	FAMILY NOT NAMED	coord: 73..168 score: 7.4
None	No IPR available	PANTHER	PTHR34410:SF2	SUBFAMILY NOT NAMED	coord: 73..168 score: 7.4

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016758	transferase activity, transferring hexosyl groups