Spo14061.1 (mRNA) | SpinachBase

Overview


Name	Spo14061.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Werner Syndrome-like exonuclease
Location	SpoScf_03351 : 14705 .. 15400 (+)
Sequence length	696

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCAGCGGAGGCGGCAACGGCAACTGTAGTAGAACCGGTAACAATAACCATCGCCGACGCAATATCAAATCCCGATCAGAACCTCTTCGATGTCCATTTCAACCGCAACCACCACATCCAAACCACCGTGACCTCCGACCCATCCGTCGTCGCATCCTGGCTCTCCTCTCTCCCCTCCTCTACCACCACCTCTCCTCTCATCGTCGGCCTCGACATCGAATGGCGCCCCAACTTCGGCCGCGCTTACGACAACCCCGTCGCAACCCTCCAACTCTGTACCTCCGCCAATCAATCTCTCTCCTCCACCTGCCTCATCTTCCAAATCCTCTACTGCCCGCCCCCAATCCCAAAACCCCTCTCCGATTTCCTCTCAAGCGATAACAACGTTTTCCTCGGCGTGGGGATTGGGTCAGACGTTGAACAACTCCTCGTAGATTACGATCTGAACGTGAAGAACACCGTCGATCTGGGGAAGCTTGCGGTTGAGAAGCTTCCAGGTGTCAGAAAAGGGATGGGGTTGAAGGAATTGGCAAGGATTGTGATGGAAGTTGAAGTTCAGAAGCCGAAAAGAGTGACACTGAGTAGGTGGGATACTGAATGGCTAAGTTATCCTCAGATTCAATATGCTTGTATTGATGCTTACTTGTCTTTTGAAATTGGCAAGAAATTATTAGCTGTTAATTCGTCTTAA

mRNA sequence

ATGGCAGCAGCGGAGGCGGCAACGGCAACTGTAGTAGAACCGGTAACAATAACCATCGCCGACGCAATATCAAATCCCGATCAGAACCTCTTCGATGTCCATTTCAACCGCAACCACCACATCCAAACCACCGTGACCTCCGACCCATCCGTCGTCGCATCCTGGCTCTCCTCTCTCCCCTCCTCTACCACCACCTCTCCTCTCATCGTCGGCCTCGACATCGAATGGCGCCCCAACTTCGGCCGCGCTTACGACAACCCCGTCGCAACCCTCCAACTCTGTACCTCCGCCAATCAATCTCTCTCCTCCACCTGCCTCATCTTCCAAATCCTCTACTGCCCGCCCCCAATCCCAAAACCCCTCTCCGATTTCCTCTCAAGCGATAACAACGTTTTCCTCGGCGTGGGGATTGGGTCAGACGTTGAACAACTCCTCGTAGATTACGATCTGAACGTGAAGAACACCGTCGATCTGGGGAAGCTTGCGGTTGAGAAGCTTCCAGGTGTCAGAAAAGGGATGGGGTTGAAGGAATTGGCAAGGATTGTGATGGAAGTTGAAGTTCAGAAGCCGAAAAGAGTGACACTGAGTAGGTGGGATACTGAATGGCTAAGTTATCCTCAGATTCAATATGCTTGTATTGATGCTTACTTGTCTTTTGAAATTGGCAAGAAATTATTAGCTGTTAATTCGTCTTAA

Coding sequence (CDS)

ATGGCAGCAGCGGAGGCGGCAACGGCAACTGTAGTAGAACCGGTAACAATAACCATCGCCGACGCAATATCAAATCCCGATCAGAACCTCTTCGATGTCCATTTCAACCGCAACCACCACATCCAAACCACCGTGACCTCCGACCCATCCGTCGTCGCATCCTGGCTCTCCTCTCTCCCCTCCTCTACCACCACCTCTCCTCTCATCGTCGGCCTCGACATCGAATGGCGCCCCAACTTCGGCCGCGCTTACGACAACCCCGTCGCAACCCTCCAACTCTGTACCTCCGCCAATCAATCTCTCTCCTCCACCTGCCTCATCTTCCAAATCCTCTACTGCCCGCCCCCAATCCCAAAACCCCTCTCCGATTTCCTCTCAAGCGATAACAACGTTTTCCTCGGCGTGGGGATTGGGTCAGACGTTGAACAACTCCTCGTAGATTACGATCTGAACGTGAAGAACACCGTCGATCTGGGGAAGCTTGCGGTTGAGAAGCTTCCAGGTGTCAGAAAAGGGATGGGGTTGAAGGAATTGGCAAGGATTGTGATGGAAGTTGAAGTTCAGAAGCCGAAAAGAGTGACACTGAGTAGGTGGGATACTGAATGGCTAAGTTATCCTCAGATTCAATATGCTTGTATTGATGCTTACTTGTCTTTTGAAATTGGCAAGAAATTATTAGCTGTTAATTCGTCTTAA

Protein sequence

MAAAEAATATVVEPVTITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLPSSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELARIVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNSS

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo14061	Spo14061	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo14061.1	Spo14061.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo14061.1.exon.1	Spo14061.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo14061.1.CDS.1	Spo14061.1.CDS.1	CDS

Homology

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BLAST of Spo14061.1 vs. NCBI nr
Match: gi|902205092|gb|KNA15087.1| (hypothetical protein SOVF_101130 [Spinacia oleracea])

HSP 1 Score: 456.8 bits (1174), Expect = 2.200e-125
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 1


		  
Query: 1   MAAAEAATATVVEPVTITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLP 60
           MAAAEAATATVVEPVTITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLP
Sbjct: 1   MAAAEAATATVVEPVTITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLP 60

Query: 61  SSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKP 120
           SSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKP
Sbjct: 61  SSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKP 120

Query: 121 LSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELAR 180
           LSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELAR
Sbjct: 121 LSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELAR 180

Query: 181 IVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNSS 232
           IVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNSS
Sbjct: 181 IVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNSS 231

[Close]

BLAST of Spo14061.1 vs. NCBI nr
Match: gi|731324703|ref|XP_010673108.1| (PREDICTED: Werner Syndrome-like exonuclease [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 318.2 bits (814), Expect = 1.200e-83
Identity = 159/222 (71.62%), Postives = 183/222 (82.43%), Query Frame = 1


		  
Query: 16  TITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLPSSTTTSPLIVGLDIE 75
           TITI+DA  NP+ NLF++ FN  H I TTVTSDPSV ++WL+SLPSS T  P +VGLDIE
Sbjct: 6   TITISDATPNPNHNLFNILFNTTHFISTTVTSDPSVASAWLASLPSSPT--PRLVGLDIE 65

Query: 76  WRPNFGRAYDNPVATLQLCTSA-------NQSLSSTCLIFQILYCPPPIPKPLSDFLSSD 135
           WRPNFGR +DNPVATLQLCT+        N SLS TCLIFQI++CPPP+PK LSDFLS++
Sbjct: 66  WRPNFGRNFDNPVATLQLCTTTTTTTTTNNHSLSYTCLIFQIIHCPPPLPKSLSDFLSNE 125

Query: 136 NNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELARIVMEVEVQ 195
           NNVF GVGI SDVEQLLVDYDL VKN VDLG LAVEK+   RKGMGLKEL RI+M+VEV 
Sbjct: 126 NNVFAGVGIRSDVEQLLVDYDLGVKNLVDLGVLAVEKIVDARKGMGLKELTRILMDVEVN 185

Query: 196 KPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNS 231
           KPK VTLSRWD+EWLSY QIQYAC+DA+LSFEIG+ L+A+ S
Sbjct: 186 KPKSVTLSRWDSEWLSYAQIQYACVDAFLSFEIGRNLMALAS 225

[Close]

BLAST of Spo14061.1 vs. NCBI nr
Match: gi|1012114599|ref|XP_015960836.1| (PREDICTED: Werner Syndrome-like exonuclease [Arachis duranensis])

HSP 1 Score: 197.2 bits (500), Expect = 3.100e-47
Identity = 109/204 (53.43%), Postives = 141/204 (69.12%), Query Frame = 1


		  
Query: 27  DQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSL----PSSTTTSPLIVGLDIEWRPNFGR 86
           D NL+DV F+  H I T +T+ PS+V +WLS       SSTTT+ ++VGLDIEWRPN  R
Sbjct: 35  DYNLYDVIFH-THTIHTLLTTSPSLVDTWLSDTLNHYHSSTTTTTVLVGLDIEWRPNTHR 94

Query: 87  AYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSDNNVFLGVGIGSDVE 146
             DNPVATLQLC      + ++CLIFQIL+  P +P+ L DFL ++ + FLGVGI  DVE
Sbjct: 95  NSDNPVATLQLC------IGNSCLIFQILH-SPSVPRSLVDFLVNEGHTFLGVGIEEDVE 154

Query: 147 QLLVDYDLNVKNTVDLGKLAVEKL-PGVRKGMGLKELARIVMEVEVQKPKRVTLSRWDTE 206
           +LL DY+LNVKN VDL  LA   L     K  GLK LA  V+ +E++KPKRV+ SRWD  
Sbjct: 155 KLLEDYNLNVKNFVDLRGLAESVLGESEMKRAGLKRLAERVLGLEIEKPKRVSRSRWDNA 214

Query: 207 WLSYPQIQYACIDAYLSFEIGKKL 226
           WL+  Q+QYAC+DA++S E+G+ L
Sbjct: 215 WLTAEQVQYACVDAFVSLEVGRML 230

[Close]

BLAST of Spo14061.1 vs. NCBI nr
Match: gi|1021483340|ref|XP_016184712.1| (PREDICTED: Werner Syndrome-like exonuclease [Arachis ipaensis])

HSP 1 Score: 196.1 bits (497), Expect = 7.000e-47
Identity = 107/204 (52.45%), Postives = 141/204 (69.12%), Query Frame = 1


		  
Query: 27  DQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSL----PSSTTTSPLIVGLDIEWRPNFGR 86
           D NL+DV F+  H I T +T+ PS+V +WLS       SSTTT+ ++VGLDIEWRPN  R
Sbjct: 35  DYNLYDVIFH-THTIHTLLTTSPSLVDTWLSDTLNHHSSSTTTTTVLVGLDIEWRPNAHR 94

Query: 87  AYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSDNNVFLGVGIGSDVE 146
             +NPVATLQLC      + + CL+FQIL+ P  +P+ L DFL ++ + FLGVG+  DVE
Sbjct: 95  NSNNPVATLQLC------IGNRCLVFQILHSPS-VPQSLVDFLVNEGHTFLGVGVEEDVE 154

Query: 147 QLLVDYDLNVKNTVDLGKLAVEKL-PGVRKGMGLKELARIVMEVEVQKPKRVTLSRWDTE 206
           +LL DY+LNVKN VDL  LA   L     K  GLK LA  V+ +E++KPKR++ SRWD  
Sbjct: 155 KLLEDYNLNVKNFVDLRDLAENVLGESEMKRAGLKSLAERVLGLEIEKPKRISRSRWDNA 214

Query: 207 WLSYPQIQYACIDAYLSFEIGKKL 226
           WL+  Q+QYACIDA++SFE+G+ L
Sbjct: 215 WLTAEQVQYACIDAFVSFEVGRML 230

[Close]

BLAST of Spo14061.1 vs. NCBI nr
Match: gi|1012029302|ref|XP_015951644.1| (PREDICTED: Werner Syndrome-like exonuclease [Arachis duranensis])

HSP 1 Score: 194.9 bits (494), Expect = 1.600e-46
Identity = 107/204 (52.45%), Postives = 140/204 (68.63%), Query Frame = 1


		  
Query: 27  DQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSL----PSSTTTSPLIVGLDIEWRPNFGR 86
           D NL+DV F+  H I T +T+ PS V +WLS       SSTTT+ ++VGLDIEWRPN  R
Sbjct: 35  DYNLYDVIFH-THTIHTLLTTSPSRVDTWLSDTLNHHSSSTTTTTVLVGLDIEWRPNSHR 94

Query: 87  AYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSDNNVFLGVGIGSDVE 146
             +NPVATLQLC      + + CL+FQIL+ P  +P+ L DFL ++ + FLGVG+  DVE
Sbjct: 95  NSNNPVATLQLC------IGNRCLVFQILHSPS-VPQSLVDFLVNEGHTFLGVGVEEDVE 154

Query: 147 QLLVDYDLNVKNTVDLGKLAVEKL-PGVRKGMGLKELARIVMEVEVQKPKRVTLSRWDTE 206
           +LL DY+LNVKN VDL  LA   L     K  GLK LA  V+ +E++KPKR++ SRWD  
Sbjct: 155 KLLEDYNLNVKNFVDLRNLAENVLGESEMKRAGLKSLAERVLGLEIEKPKRISRSRWDNA 214

Query: 207 WLSYPQIQYACIDAYLSFEIGKKL 226
           WL+  Q+QYACIDA++SFE+G+ L
Sbjct: 215 WLTAEQVQYACIDAFVSFEVGRML 230

[Close]

BLAST of Spo14061.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R8J7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_101130 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 1.500e-125
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 1


		  
Query: 1   MAAAEAATATVVEPVTITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLP 60
           MAAAEAATATVVEPVTITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLP
Sbjct: 1   MAAAEAATATVVEPVTITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLP 60

Query: 61  SSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKP 120
           SSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKP
Sbjct: 61  SSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKP 120

Query: 121 LSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELAR 180
           LSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELAR
Sbjct: 121 LSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELAR 180

Query: 181 IVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNSS 232
           IVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNSS
Sbjct: 181 IVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNSS 231

[Close]

BLAST of Spo14061.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CP14_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g063710 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 8.500e-84
Identity = 159/222 (71.62%), Postives = 183/222 (82.43%), Query Frame = 1


		  
Query: 16  TITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLPSSTTTSPLIVGLDIE 75
           TITI+DA  NP+ NLF++ FN  H I TTVTSDPSV ++WL+SLPSS T  P +VGLDIE
Sbjct: 6   TITISDATPNPNHNLFNILFNTTHFISTTVTSDPSVASAWLASLPSSPT--PRLVGLDIE 65

Query: 76  WRPNFGRAYDNPVATLQLCTSA-------NQSLSSTCLIFQILYCPPPIPKPLSDFLSSD 135
           WRPNFGR +DNPVATLQLCT+        N SLS TCLIFQI++CPPP+PK LSDFLS++
Sbjct: 66  WRPNFGRNFDNPVATLQLCTTTTTTTTTNNHSLSYTCLIFQIIHCPPPLPKSLSDFLSNE 125

Query: 136 NNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELARIVMEVEVQ 195
           NNVF GVGI SDVEQLLVDYDL VKN VDLG LAVEK+   RKGMGLKEL RI+M+VEV 
Sbjct: 126 NNVFAGVGIRSDVEQLLVDYDLGVKNLVDLGVLAVEKIVDARKGMGLKELTRILMDVEVN 185

Query: 196 KPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVNS 231
           KPK VTLSRWD+EWLSY QIQYAC+DA+LSFEIG+ L+A+ S
Sbjct: 186 KPKSVTLSRWDSEWLSYAQIQYACVDAFLSFEIGRNLMALAS 225

[Close]

BLAST of Spo14061.1 vs. UniProtKB/TrEMBL
Match: W9SHV7_9ROSA (Werner Syndrome-like exonuclease OS=Morus notabilis GN=L484_027884 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.600e-45
Identity = 107/215 (49.77%), Postives = 148/215 (68.84%), Query Frame = 1


		  
Query: 17  ITIADAISNPD-QNLFDVHFNRNHHIQTTVTSDPSVVASWLSSLPS--STTTSPLIVGLD 76
           +TI D   + D  NL+DV    +  ++T +T+ PS+V+SW+S +        S LIVGLD
Sbjct: 4   VTIHDHELDSDIHNLYDVVIY-SKQVKTLLTTCPSMVSSWISDVEKIHRRRLSRLIVGLD 63

Query: 77  IEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSDNNVFL 136
           +EWRP+F R Y NPVATLQ+C      +   CLIFQ+++    IP  LSDFL++ N  F+
Sbjct: 64  VEWRPSFSR-YQNPVATLQIC------VGHRCLIFQLIHADS-IPASLSDFLANANYSFV 123

Query: 137 GVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKL--PGVRKGMGLKELARIVMEVEVQKPK 196
           GVGIG+D+E+L  D+ L V+N VDLG LA  +   PG+ K  GLK+LARIV  +E++KPK
Sbjct: 124 GVGIGADIEKLAEDHGLTVRNAVDLGDLAAREYDDPGM-KNRGLKDLARIVRGMEIEKPK 183

Query: 197 RVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLL 227
           R+T+ RWD  WL+  Q+QYAC+DA++SFEIGKKL+
Sbjct: 184 RITMGRWDNRWLTCEQVQYACVDAFVSFEIGKKLI 208

[Close]

BLAST of Spo14061.1 vs. UniProtKB/TrEMBL
Match: B9SNW3_RICCO (3-5 exonuclease, putative OS=Ricinus communis GN=RCOM_0582440 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.900e-45
Identity = 107/215 (49.77%), Postives = 145/215 (67.44%), Query Frame = 1


		  
Query: 15  VTITIAD-AISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWL-SSLPSSTTTSPLIVGL 74
           +T+ I D  + N   NL+D+ F  +  I T VT  PS+V  WL  +   +  T P IVGL
Sbjct: 1   MTLGIRDYELINDTHNLYDITFFTDQ-IHTLVTHSPSLVEQWLIETQGQNNQTQPTIVGL 60

Query: 75  DIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSDNNVF 134
           D+EWRPNF R  +NPVATLQLC      + S CLI+Q+++ P  IP+ L DFL + + VF
Sbjct: 61  DVEWRPNFSRHIENPVATLQLC------IGSRCLIYQLIHSPR-IPQSLFDFLKNSSYVF 120

Query: 135 LGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVR--KGMGLKELARIVMEVEVQKP 194
            GVGI SDVE+L+ DY L+V N ++L ++A E L GV+  K  GLKEL + V+  E+QKP
Sbjct: 121 AGVGIESDVEKLVEDYGLSVGNVMELRRVAAESL-GVKELKNAGLKELVKQVLGKEIQKP 180

Query: 195 KRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKL 226
           KRVT+SRWD+ WLS+ Q+QYAC+DA++  EIG+ L
Sbjct: 181 KRVTMSRWDSMWLSHDQVQYACLDAFVCSEIGRIL 206

[Close]

BLAST of Spo14061.1 vs. UniProtKB/TrEMBL
Match: A0A0R0J373_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G143200 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.300e-44
Identity = 109/234 (46.58%), Postives = 144/234 (61.54%), Query Frame = 1


		  
Query: 1   MAAAEAATATVVEPVTITIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSL- 60
           ++++   T  VV P+++ +   +     NL+DV FN  H I T +TSDPS+V SW+S++ 
Sbjct: 13  LSSSTTTTTPVVAPISV-VDHGLPYDTHNLYDVSFNNTHTIYTLLTSDPSLVDSWISTVL 72

Query: 61  ---PSSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPP 120
                      L VGLDIEWRPN  R   NPVATLQLC      ++  CL+FQIL+  P 
Sbjct: 73  RDHQHQHQQRVLTVGLDIEWRPNTQRNMQNPVATLQLC------VAERCLVFQILH-SPS 132

Query: 121 IPKPLSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVR-KGMGL 180
           IP  L  FL+  N  F+GVGI  DVE+LL DY+LNV N  DL   A E+L  +  K  GL
Sbjct: 133 IPPSLVSFLADPNITFVGVGIQEDVEKLLEDYNLNVANVRDLRSFAAERLGDLELKRAGL 192

Query: 181 KELARIVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLLAVN 230
           K L   V+ +EV KPKRVT SRWD  WL+  Q+QYA +DA+LS+EI ++L + N
Sbjct: 193 KSLGLRVLGLEVAKPKRVTRSRWDNPWLTAQQVQYAAVDAFLSYEIDRRLSSYN 238

[Close]

BLAST of Spo14061.1 vs. ExPASy Swiss-Prot
Match: WEX_ARATH (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.100e-18
Identity = 58/157 (36.94%), Postives = 85/157 (54.14%), Query Frame = 1


		  
Query: 70  VGLDIEWRPNFGRAY-DNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSD 129
           VGLDIEWRP+F +      VAT+Q+C  +N      C +  I +    IP+ L   +   
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNY-----CDVMHIFHSG--IPQSLQHLIEDS 189

Query: 130 NNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELARIVMEVEVQ 189
             V +G+GI  D  +L  DY +++K+  DL  LA +K+ G +K  GL  L   ++  E+ 
Sbjct: 190 TLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGDKK-WGLASLTETLVCKELL 249

Query: 190 KPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKL 226
           KP R+ L  W+   LS  Q+QYA  DAY S+ + K L
Sbjct: 250 KPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Spo14061.1 vs. ExPASy Swiss-Prot
Match: WRN_XENLA (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.600e-12
Identity = 49/157 (31.21%), Postives = 77/157 (49.04%), Query Frame = 1


		  
Query: 69  IVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSD 128
           ++G DIEW P + +     VA +Q+C S  +     C +F I       PK L   L  +
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKK-----CYLFHISPMAG-FPKGLKRLLEDE 131

Query: 129 NNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELARIVMEVEVQ 188
           +   +GVGI  D  +L+ DY+L +K  ++L ++A +KL   ++      L + + + ++ 
Sbjct: 132 SVRKVGVGIEGDQWKLMSDYELKLKGFIELSEMANQKL-RCKEKWTFNGLIKHLFKEQLY 191

Query: 189 KPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKL 226
           K K    S WD   L+  Q  YA  DAY    I KKL
Sbjct: 192 KRKSYRCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

[Close]

BLAST of Spo14061.1 vs. ExPASy Swiss-Prot
Match: EXD2_MOUSE (Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus GN=Exd2 PE=1 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 9.500e-12
Identity = 47/159 (29.56%), Postives = 83/159 (52.20%), Query Frame = 1


		  
Query: 69  IVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSD 128
           ++G+D EW    G+A  +P++ LQ+ + +     +   + +++Y    +P+ L D L+  
Sbjct: 133 VLGIDCEWVNLEGKA--SPLSLLQMASPSG--FCALVRLPRLIYGGRTLPRTLLDILADG 192

Query: 129 NNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVR-KGMGLKELARIVMEVEV 188
             + +GVG   D  +LL DY L V+  +DL  LA+++   +   G+ LK LA  ++   +
Sbjct: 193 AILKVGVGCSEDANKLLQDYGLIVRGCLDLRYLAMKQGNNILCNGLSLKSLAETILNFPL 252

Query: 189 QKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLL 227
            K   +  S WD E L+  Q+ YA  DA +S  +   LL
Sbjct: 253 DKSLLLRCSNWDAENLTEDQVTYAARDAQISVALFLHLL 287

[Close]

BLAST of Spo14061.1 vs. ExPASy Swiss-Prot
Match: EXD2_HUMAN (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 9.500e-12
Identity = 52/162 (32.10%), Postives = 83/162 (51.23%), Query Frame = 1


		  
Query: 69  IVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQI--LYCP-PPIPKPLSDFL 128
           ++G+D EW    G+A  +P++ LQ+      S S  C++ ++  L C    +P+ L D L
Sbjct: 104 VLGIDCEWVNLEGKA--SPLSLLQMA-----SPSGLCVLVRLPKLICGGKTLPRTLLDIL 163

Query: 129 SSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVR-KGMGLKELARIVME 188
           +    + +GVG   D  +LL DY L V+  +DL  LA+ +   +   G+ LK LA  V+ 
Sbjct: 164 ADGTILKVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQRNNLLCNGLSLKSLAETVLN 223

Query: 189 VEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKLL 227
             + K   +  S WD E L+  Q+ YA  DA +S  +   LL
Sbjct: 224 FPLDKSLLLRCSNWDAETLTEDQVIYAARDAQISVALFLHLL 258

[Close]

BLAST of Spo14061.1 vs. ExPASy Swiss-Prot
Match: WRN_HUMAN (Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 9.500e-12
Identity = 55/172 (31.98%), Postives = 82/172 (47.67%), Query Frame = 1


		  
Query: 54  SWLSSLPSSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQSLSSTCLIFQILYC 113
           S+LS   S + +   +VG D+EW P + R     VA +QLC S      S C +F +   
Sbjct: 63  SFLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVS-----ESKCYLFHVSSM 122

Query: 114 PPPIPKPLSDFLSSDNNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGM 173
               P+ L   L +      GVGI  D  +LL D+D+ +KN V+L  +A +KL    +  
Sbjct: 123 SV-FPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLK-CTETW 182

Query: 174 GLKELARIVMEVEVQKPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKL 226
            L  L + ++  ++ K K +  S W    L+  Q  YA  DAY  F I + L
Sbjct: 183 SLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNL 227

[Close]

BLAST of Spo14061.1 vs. TAIR (Arabidopsis)
Match: AT4G13870.2 (Werner syndrome-like exonuclease)

HSP 1 Score: 95.1 bits (235), Expect = 5.900e-20
Identity = 58/157 (36.94%), Postives = 85/157 (54.14%), Query Frame = 1


		  
Query: 70  VGLDIEWRPNFGRAY-DNPVATLQLCTSANQSLSSTCLIFQILYCPPPIPKPLSDFLSSD 129
           VGLDIEWRP+F +      VAT+Q+C  +N      C +  I +    IP+ L   +   
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNY-----CDVMHIFHSG--IPQSLQHLIEDS 189

Query: 130 NNVFLGVGIGSDVEQLLVDYDLNVKNTVDLGKLAVEKLPGVRKGMGLKELARIVMEVEVQ 189
             V +G+GI  D  +L  DY +++K+  DL  LA +K+ G +K  GL  L   ++  E+ 
Sbjct: 190 TLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGDKK-WGLASLTETLVCKELL 249

Query: 190 KPKRVTLSRWDTEWLSYPQIQYACIDAYLSFEIGKKL 226
           KP R+ L  W+   LS  Q+QYA  DAY S+ + K L
Sbjct: 250 KPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Spo14061.1 vs. TAIR (Arabidopsis)
Match: AT3G12460.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 65.9 bits (159), Expect = 3.800e-11
Identity = 63/232 (27.16%), Postives = 99/232 (42.67%), Query Frame = 1


		  
Query: 18  TIADAISNPDQNLFDVHFNRNHHIQTTVTSDPSVVASWLSSL--PSSTTTSPLIVGLDIE 77
           TI    S   Q  + V F     +  TVT DPSV+  W+  +   +  ++ PL+VG+ ++
Sbjct: 4   TIRTIGSYATQQRYSVDFF-GEELFVTVTPDPSVIGQWIHDVFFHNRLSSHPLVVGVGVQ 63

Query: 78  WR--------------------------PNFGRAYDNPVATLQLCTSANQSLSSTCLIFQ 137
           W                           P    + D P  TLQLC      + + C+I Q
Sbjct: 64  WTPSGYHPASPPVSYRSDSSPDSYRSDSPPVFYSSDPPADTLQLC------VGNRCIIIQ 123

Query: 138 ILYCPPPIPKPLSDFLSSDNNVFLGVGIGSDVEQL-LVDYDLNVKNTVDLGKLAVEKLPG 197
           + YC   +P+ L +FL+  +N F+G+    D  +L    + L +   +DL +   +    
Sbjct: 124 LRYCER-VPQVLRNFLADRDNTFVGIWNSQDAGKLERSRHQLEIAELMDLREFVSDS--S 183

Query: 198 VRKGMGLKELARIVME----VEVQKPKRVTLSRWDTEWLSYPQIQYACIDAY 217
            R+ M    L +IV E      V+  + V++S W    LSY QI  A ID Y
Sbjct: 184 GRRSMYNYSLEKIVEENLGYPGVRLDREVSMSDWRVYNLSYDQILQASIDVY 225

[Close]

BLAST of Spo14061.1 vs. TAIR (Arabidopsis)
Match: AT5G48350.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 63.5 bits (153), Expect = 1.900e-10
Identity = 60/199 (30.15%), Postives = 87/199 (43.72%), Query Frame = 1


		  
Query: 41  IQTTVTSDPSVVASWLSSLPSSTTTSPLIVGLDIEWRPNFGRAYDNPVATLQLCTSANQS 100
           I+TTVT     +   + +  S+      I+GLD E R   GR   N    LQLC   N  
Sbjct: 13  IKTTVTEKERDINRLVKTFLSNKNNRKKIIGLDTE-RVQKGRKL-NKTVLLQLCDGDN-- 72

Query: 101 LSSTCLIFQILYCPPP-----------IPKPLSDFLSSDNNVFLGVGIGSDVEQLLVDYD 160
               CLI Q+   P             +P PL +FL+     F+G+GI   + +L  ++ 
Sbjct: 73  ----CLIVQL---PDEDEDEGEGEDDNLPLPLFNFLNLPEFTFVGIGINKTMMRLESEFG 132

Query: 161 LNVKNTVDLGKLAVEKLPGVRKGMGLKELARIVMEVEVQKPKRVTLSRWDTEWLSYPQIQ 220
           L  KN V++G  A   L  +   +      RI   V  ++P    L  W+   L+  QI+
Sbjct: 133 LTCKNVVEIGP-ATWNLTNMTTDVKF----RISAIVSTERPSNAVLEDWEKFVLNKNQIK 192

Query: 221 YACIDAYLSFEIGKKLLAV 229
            A  +AY +F IG  LL V
Sbjct: 193 LAASNAYFAFGIGNILLDV 195

The following BLAST results are available for this feature:

BLAST of Spo14061.1 vs. NCBI nr
BLAST of Spo14061.1 vs. UniProtKB/TrEMBL
BLAST of Spo14061.1 vs. ExPASy Swiss-Prot
BLAST of Spo14061.1 vs. TAIR (Arabidopsis)

BLAST of Spo14061.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902205092\|gb\|KNA15087.1\|	2.2e-125	100.	hypothetical protein SOVF_1011...	[more]
gi\|731324703\|ref\|XP_010673108.1\|	1.2e-83	71.6	PREDICTED: Werner Syndrome-lik...	[more]
gi\|1012114599\|ref\|XP_015960836.1\|	3.1e-47	53.4	PREDICTED: Werner Syndrome-lik...	[more]
gi\|1021483340\|ref\|XP_016184712.1\|	7.0e-47	52.4	PREDICTED: Werner Syndrome-lik...	[more]
gi\|1012029302\|ref\|XP_015951644.1\|	1.6e-46	52.4	PREDICTED: Werner Syndrome-lik...	[more]

BLAST of Spo14061.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R8J7_SPIOL	1.5e-125	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CP14_BETVU	8.5e-84	71.6	Uncharacterized protein OS=Bet...	[more]
W9SHV7_9ROSA	1.6e-45	49.7	Werner Syndrome-like exonuclea...	[more]
B9SNW3_RICCO	5.9e-45	49.7	3-5 exonuclease, putative OS=R...	[more]
A0A0R0J373_SOYBN	2.3e-44	46.5	Uncharacterized protein OS=Gly...	[more]

BLAST of Spo14061.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
WEX_ARATH	1.1e-18	36.9	Werner Syndrome-like exonuclea...	[more]
WRN_XENLA	5.6e-12	31.2	Werner syndrome ATP-dependent ...	[more]
EXD2_MOUSE	9.5e-12	29.5	Exonuclease 3'-5' domain-conta...	[more]
EXD2_HUMAN	9.5e-12	32.1	Exonuclease 3'-5' domain-conta...	[more]
WRN_HUMAN	9.5e-12	31.9	Werner syndrome ATP-dependent ...	[more]

BLAST of Spo14061.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G13870.2	5.9e-20	36.9	Werner syndrome-like exonuclea...	[more]
AT3G12460.1	3.8e-11	27.1	Polynucleotidyl transferase, r...	[more]
AT5G48350.1	1.9e-10	30.1	Polynucleotidyl transferase, r...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 56..226 score: 6.0
IPR012337	Ribonuclease H-like domain	GENE3D	3.30.420.10		coord: 26..226 score: 4.6
IPR012337	Ribonuclease H-like domain	unknown	SSF53098	Ribonuclease H-like	coord: 66..226 score: 1.35
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 18..226 score: 2.6

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding