Spo10762.1 (mRNA) | SpinachBase

Overview


Name	Spo10762.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Pectin lyase-like protein
Location	chr1 : 41261263 .. 41262564 (-)
Sequence length	725

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAACCAACTCTTCCACTAGTGTAGCTGATACTTTGGTATATTGTGTTAGATTATGGGAGAGTGTGATCTGTAGCTCCATCTTCATGGTCCTTTTACTTTCCGTTGTTGCACCTGAAAGAAGCTATTAAGGAATCGCTTCTGTACAAGAAATCTGAAACTCAATTCACAAGCAAGGAAGTTATGCCTAAGAAAAAGCAAGCTTAGTTAAGCAAAGAATCTCAAATGCTGTACTGATATAGTGTTACCCTTCCCTCTCTAATACTACAGCAGTTCTGCTCTGATGATTGAATAAAATAGCACTTTGGTCAAGAATCCATGTGCTTACTAATTTAAGCTCCTAGATAATGATTAATATAGGAAAGCAAGGAAACTCCAGCTGAAATAGACCTTTTTATAGCATTTCCTTTGAAAAGGGAAAGCGTGTTTTTGCTTCTTTTTTCTTCATTTTATTAAGGTCGAAAAGGTATCCATTCAATGGAAGCAAGAATTTATGTAATGATGTGTCTTTCTTTCGTTTTTCTTCTTTCCATATTTTAACTTTCCCCCTTTTCTAGTTTATGATCACATGATCAAGTTGCCTAATCTTTTCTTGATTTCCTTTTGGTTTTTCAGTCGACTGCTGTGCAAATTAGTCAGGTGATGTACCGGAATATTACTGGAACTACAAAGAGCAAAGAAGCCATGAAGTTTGCTTGCAGCGACTCGGTTCCTTGCAGGAACATTGTTCTCTCTAATATTGACTTGCACAAGAAGGATGGCACAGCAGAGACATACTGTAACTCTGTTACTGGTTTTGGTTTCGGAACAATCAATCCCTCAGCTGATTGCCTTACCTCCAATGACAAAGATTATTCCAATCCTGATGACATGAAAATTTCCCAACTCGGGGAATCGATTGAAGATCACATAGTTCATACAGAGCTGTGATTTTGCTTCCATCATATTATTATAGGTACTAATATAGCAGCTTAGTAATGGGTTTATTAAACTCAAATGGTATTTTAAATGATGTAGAATACATACTTTAGGGAAAGGTGTTAGTTCCATTTCTAAAATTTTAAGTTTTCCTATTTTCTTTTTCTGATTTGGATTGTAATGATGCAATAGCAATATCTTATTTGTAATTGATTGTTATTATTGAAGCATTATTGAAAGATTGTGTTATTGAATTTGTTGGTGAGAATGAAAATTTATCGCTTCAATCGTAGTTCAACTTATGTAGCACAAATTCACAAAAGCTGTATGTATATCAAGGGTTTGTTGTGAGAATAGTGAAACATCCGGGGACTTGAGGCCAG

mRNA sequence

ATGAAAACCAACTCTTCCACTAGTGTAGCTGATACTTTGTCGACTGCTGTGCAAATTAGTCAGGTGATGTACCGGAATATTACTGGAACTACAAAGAGCAAAGAAGCCATGAAGTTTGCTTGCAGCGACTCGGTTCCTTGCAGGAACATTGTTCTCTCTAATATTGACTTGCACAAGAAGGATGGCACAGCAGAGACATACTGTAACTCTGTTACTGGTTTTGGTTTCGGAACAATCAATCCCTCAGCTGATTGCCTTACCTCCAATGACAAAGATTATTCCAATCCTGATGACATGAAAATTTCCCAACTCGGGGAATCGATTGAAGATCACATAGTTCATACAGAGCTGTGATTTTGCTTCCATCATATTATTATAGGTACTAATATAGCAGCTTAGTAATGGGTTTATTAAACTCAAATGGTATTTTAAATGATGTAGAATACATACTTTAGGGAAAGGTGTTAGTTCCATTTCTAAAATTTTAAGTTTTCCTATTTTCTTTTTCTGATTTGGATTGTAATGATGCAATAGCAATATCTTATTTGTAATTGATTGTTATTATTGAAGCATTATTGAAAGATTGTGTTATTGAATTTGTTGGTGAGAATGAAAATTTATCGCTTCAATCGTAGTTCAACTTATGTAGCACAAATTCACAAAAGCTGTATGTATATCAAGGGTTTGTTGTGAGAATAGTGAAACATCCGGGGACTTGAGGCCAG

Coding sequence (CDS)

ATGAAAACCAACTCTTCCACTAGTGTAGCTGATACTTTGTCGACTGCTGTGCAAATTAGTCAGGTGATGTACCGGAATATTACTGGAACTACAAAGAGCAAAGAAGCCATGAAGTTTGCTTGCAGCGACTCGGTTCCTTGCAGGAACATTGTTCTCTCTAATATTGACTTGCACAAGAAGGATGGCACAGCAGAGACATACTGTAACTCTGTTACTGGTTTTGGTTTCGGAACAATCAATCCCTCAGCTGATTGCCTTACCTCCAATGACAAAGATTATTCCAATCCTGATGACATGAAAATTTCCCAACTCGGGGAATCGATTGAAGATCACATAGTTCATACAGAGCTGTGA

Protein sequence

MKTNSSTSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTGFGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo10762	Spo10762	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo10762.1	Spo10762.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10762.1.CDS.2	Spo10762.1.CDS.2	CDS
Spo10762.1.CDS.1	Spo10762.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10762.1.utr3p.1	Spo10762.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10762.1.exon.2	Spo10762.1.exon.2	exon
Spo10762.1.exon.1	Spo10762.1.exon.1	exon

Homology

[Close]

BLAST of Spo10762.1 vs. NCBI nr
Match: gi|902156880|gb|KNA06047.1| (hypothetical protein SOVF_184640 [Spinacia oleracea])

HSP 1 Score: 212.2 bits (539), Expect = 4.800e-52
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 1


		  
Query: 14  STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTG 73
           STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTG
Sbjct: 7   STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTG 66

Query: 74  FGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
           FGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL
Sbjct: 67  FGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 110

[Close]

BLAST of Spo10762.1 vs. NCBI nr
Match: gi|731342707|ref|XP_010682531.1| (PREDICTED: probable polygalacturonase At1g80170 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 193.0 bits (489), Expect = 3.000e-46
Identity = 92/104 (88.46%), Postives = 99/104 (95.19%), Query Frame = 1


		  
Query: 14  STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTG 73
           +TAVQISQVMYRNITGTT SKEAMKFACSD+ PCRNIVL+NIDLHKKDGTAETYCNSVTG
Sbjct: 361 TTAVQISQVMYRNITGTTSSKEAMKFACSDAFPCRNIVLTNIDLHKKDGTAETYCNSVTG 420

Query: 74  FGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
           FGFGTINP ADCLTSNDKDYS+PD+MKISQ+ ES EDH+VHTEL
Sbjct: 421 FGFGTINPPADCLTSNDKDYSSPDEMKISQIKESTEDHLVHTEL 464

[Close]

BLAST of Spo10762.1 vs. NCBI nr
Match: gi|728842873|gb|KHG22316.1| (hypothetical protein F383_04489 [Gossypium arboreum])

HSP 1 Score: 154.5 bits (389), Expect = 1.200e-34
Identity = 76/116 (65.52%), Postives = 91/116 (78.45%), Query Frame = 1


		  
Query: 2   KTNSSTSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKD 61
           +T +   +    ++AVQISQ+MYRNI+GTTKSKEAMKFACSD+ PC NIVLSNI+L KKD
Sbjct: 307 ETTNGVRIKTWQTSAVQISQIMYRNISGTTKSKEAMKFACSDTTPCSNIVLSNINLEKKD 366

Query: 62  GTAETYCNSVTGFGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
           GTAETYCNS  GFG+G ++PSADCL+SNDK Y   D  + S+L E   DHIVHTEL
Sbjct: 367 GTAETYCNSAQGFGYGIVHPSADCLSSNDKGYILIDHKENSELAEPSRDHIVHTEL 422

[Close]

BLAST of Spo10762.1 vs. NCBI nr
Match: gi|823159084|ref|XP_012479372.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g27110-like isoform X2 [Gossypium raimondii])

HSP 1 Score: 154.1 bits (388), Expect = 1.500e-34
Identity = 76/112 (67.86%), Postives = 88/112 (78.57%), Query Frame = 1


		  
Query: 6    STSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAE 65
            S       ++AVQISQ+MYRNI+GTTKSKEAMKFACSD+ PC NIVLSNI+L KKDGTAE
Sbjct: 993  SPKTCQNQTSAVQISQIMYRNISGTTKSKEAMKFACSDTTPCSNIVLSNINLEKKDGTAE 1052

Query: 66   TYCNSVTGFGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
            TYCNS  GFG+G ++PSADCL+SNDK Y   D  + S+L E   DHIVHTEL
Sbjct: 1053 TYCNSAQGFGYGIVHPSADCLSSNDKGYILIDHKENSELAEPTGDHIVHTEL 1104

[Close]

BLAST of Spo10762.1 vs. NCBI nr
Match: gi|763763959|gb|KJB31213.1| (hypothetical protein B456_005G181300 [Gossypium raimondii])

HSP 1 Score: 154.1 bits (388), Expect = 1.500e-34
Identity = 76/112 (67.86%), Postives = 88/112 (78.57%), Query Frame = 1


		  
Query: 6    STSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAE 65
            S       ++AVQISQ+MYRNI+GTTKSKEAMKFACSD+ PC NIVLSNI+L KKDGTAE
Sbjct: 989  SPKTCQNQTSAVQISQIMYRNISGTTKSKEAMKFACSDTTPCSNIVLSNINLEKKDGTAE 1048

Query: 66   TYCNSVTGFGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
            TYCNS  GFG+G ++PSADCL+SNDK Y   D  + S+L E   DHIVHTEL
Sbjct: 1049 TYCNSAQGFGYGIVHPSADCLSSNDKGYILIDHKENSELAEPTGDHIVHTEL 1100

[Close]

BLAST of Spo10762.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QFK6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_184640 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 3.300e-52
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 1


		  
Query: 14  STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTG 73
           STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTG
Sbjct: 7   STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTG 66

Query: 74  FGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
           FGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL
Sbjct: 67  FGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 110

[Close]

BLAST of Spo10762.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C189_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g148660 PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 2.100e-46
Identity = 92/104 (88.46%), Postives = 99/104 (95.19%), Query Frame = 1


		  
Query: 14  STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTG 73
           +TAVQISQVMYRNITGTT SKEAMKFACSD+ PCRNIVL+NIDLHKKDGTAETYCNSVTG
Sbjct: 361 TTAVQISQVMYRNITGTTSSKEAMKFACSDAFPCRNIVLTNIDLHKKDGTAETYCNSVTG 420

Query: 74  FGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
           FGFGTINP ADCLTSNDKDYS+PD+MKISQ+ ES EDH+VHTEL
Sbjct: 421 FGFGTINPPADCLTSNDKDYSSPDEMKISQIKESTEDHLVHTEL 464

[Close]

BLAST of Spo10762.1 vs. UniProtKB/TrEMBL
Match: A0A0B0PG30_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_04489 PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 8.200e-35
Identity = 76/116 (65.52%), Postives = 91/116 (78.45%), Query Frame = 1


		  
Query: 2   KTNSSTSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKD 61
           +T +   +    ++AVQISQ+MYRNI+GTTKSKEAMKFACSD+ PC NIVLSNI+L KKD
Sbjct: 307 ETTNGVRIKTWQTSAVQISQIMYRNISGTTKSKEAMKFACSDTTPCSNIVLSNINLEKKD 366

Query: 62  GTAETYCNSVTGFGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
           GTAETYCNS  GFG+G ++PSADCL+SNDK Y   D  + S+L E   DHIVHTEL
Sbjct: 367 GTAETYCNSAQGFGYGIVHPSADCLSSNDKGYILIDHKENSELAEPSRDHIVHTEL 422

[Close]

BLAST of Spo10762.1 vs. UniProtKB/TrEMBL
Match: A0A0D2PUY8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G181300 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.100e-34
Identity = 76/112 (67.86%), Postives = 88/112 (78.57%), Query Frame = 1


		  
Query: 6    STSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAE 65
            S       ++AVQISQ+MYRNI+GTTKSKEAMKFACSD+ PC NIVLSNI+L KKDGTAE
Sbjct: 989  SPKTCQNQTSAVQISQIMYRNISGTTKSKEAMKFACSDTTPCSNIVLSNINLEKKDGTAE 1048

Query: 66   TYCNSVTGFGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
            TYCNS  GFG+G ++PSADCL+SNDK Y   D  + S+L E   DHIVHTEL
Sbjct: 1049 TYCNSAQGFGYGIVHPSADCLSSNDKGYILIDHKENSELAEPTGDHIVHTEL 1100

[Close]

BLAST of Spo10762.1 vs. UniProtKB/TrEMBL
Match: A0A067JRM6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17764 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 8.500e-32
Identity = 72/123 (58.54%), Postives = 92/123 (74.80%), Query Frame = 1


		  
Query: 6   STSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAE 65
           S       ++AV+IS++MYRN+TGTTKS EAMKFACSD+VPC NIVLSNI+L KKDGTAE
Sbjct: 170 SPKTCQNQTSAVKISEIMYRNVTGTTKSAEAMKFACSDTVPCSNIVLSNINLEKKDGTAE 229

Query: 66  TYCNSVTGFGFGTINPSADCLTSNDKDYSN-----------PDDMKISQLGESIEDHIVH 118
           TYCNS  GFG+G ++P+ADCL+S+DKD+S             D+ +I++L ES  + IVH
Sbjct: 230 TYCNSAQGFGYGIVHPAADCLSSHDKDFSMVDQTEIAQDYVTDETEIAELAESSSEAIVH 289

[Close]

BLAST of Spo10762.1 vs. ExPASy Swiss-Prot
Match: PGLR5_ARATH (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 8.500e-9
Identity = 31/78 (39.74%), Postives = 48/78 (61.54%), Query Frame = 1


		  
Query: 14  STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKK--DGTAETYCNSV 73
           ++A+ I  + + ++ GT+ SKEA+K +CSDS PCRNI+L +IDL     DG  E++C   
Sbjct: 351 TSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEA 410

Query: 74  TGFGFGTINPSADCLTSN 90
            G   G + P   CL+ +
Sbjct: 411 YGSSSGQVYP-PPCLSDD 427

[Close]

BLAST of Spo10762.1 vs. ExPASy Swiss-Prot
Match: PGLR_ACTDE (Polygalacturonase OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.700e-7
Identity = 29/69 (42.03%), Postives = 42/69 (60.87%), Query Frame = 1


		  
Query: 14  STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDG-TAETYCNSVT 73
           S+AVQ+  V+Y+NI GT  S  A+ F CS   PC+ IVL ++DL  + G  A+  CN+V 
Sbjct: 385 SSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVE 444

Query: 74  GFGFGTINP 82
               G ++P
Sbjct: 445 LSETGVVSP 453

[Close]

BLAST of Spo10762.1 vs. ExPASy Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.700e-7
Identity = 29/72 (40.28%), Postives = 43/72 (59.72%), Query Frame = 1


		  
Query: 15  TAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAETYCNSVTGF 74
           + VQIS V Y +I GT+ ++ A+KF CS   PCR I L ++ L  K+  AE+ C+   G 
Sbjct: 323 SGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTYKNQAAESSCSHADGT 382

Query: 75  GFGTINPSADCL 87
             G + P++ CL
Sbjct: 383 TEGVVQPTS-CL 393

[Close]

BLAST of Spo10762.1 vs. ExPASy Swiss-Prot
Match: PGLR2_CRYJA (Polygalacturonase OS=Cryptomeria japonica PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 8.000e-7
Identity = 33/107 (30.84%), Postives = 55/107 (51.40%), Query Frame = 1


		  
Query: 5   SSTSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTA 64
           +S S      +AVQI  V Y+NI GT+ +  A++  CSDS+PC++I LS+I L    G  
Sbjct: 348 TSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

Query: 65  ETYCN-SVTGFGFGTINPSADCLT-----SNDKDYSNPDDMKISQLG 106
            +  N +  G+  G + P+   L+        K + +P  + +  +G
Sbjct: 408 ASCLNDNANGYFSGHVIPACKNLSPSAKRKESKSHKHPKTVMVKNMG 454

[Close]

BLAST of Spo10762.1 vs. ExPASy Swiss-Prot
Match: PGLR2_JUNAS (Polygalacturonase OS=Juniperus ashei GN=JNA2 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 8.000e-7
Identity = 31/83 (37.35%), Postives = 45/83 (54.22%), Query Frame = 1


		  
Query: 5   SSTSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTA 64
           +S +      +AV+I  V ++NI GT+ +  A++  CSDSVPC NI LSN+ L    G  
Sbjct: 349 TSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKV 408

Query: 65  ETYCN-SVTGFGFGTINPSADCL 87
            T  N +  G+    +NPS   L
Sbjct: 409 ATCVNKNANGYYTNPLNPSCKSL 431

[Close]

BLAST of Spo10762.1 vs. TAIR (Arabidopsis)
Match: AT1G23460.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 114.4 bits (285), Expect = 4.800e-26
Identity = 59/112 (52.68%), Postives = 76/112 (67.86%), Query Frame = 1


		  
Query: 6   STSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAE 65
           S +     ++AV+ISQ+MYRNITGTTKS +A+KFACSD+VPC +IVL+N++L   DG  E
Sbjct: 356 SPTTCQNQTSAVKISQIMYRNITGTTKSAKAIKFACSDTVPCSHIVLNNVNLEGNDGQVE 415

Query: 66  TYCNSVTGFGFGTINPSADCLTSNDKDYSNPDDMKISQLGESIEDHIVHTEL 118
            YCNS  GFG+G I+PSADCL S+       DD  + Q  +S E    H EL
Sbjct: 416 AYCNSAEGFGYGVIHPSADCLYSH-------DDKGLDQTHKSEEAETGHDEL 460

[Close]

BLAST of Spo10762.1 vs. TAIR (Arabidopsis)
Match: AT1G70500.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 110.5 bits (275), Expect = 6.900e-25
Identity = 52/98 (53.06%), Postives = 69/98 (70.41%), Query Frame = 1


		  
Query: 6   STSVADTLSTAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGTAE 65
           S S     ++AV IS++MYRNITGTTKS +A+ F CSD+VPC +IVL+NI+L   DG  E
Sbjct: 356 SPSTCQNQTSAVHISEIMYRNITGTTKSSKAINFKCSDAVPCSHIVLNNINLEGNDGKVE 415

Query: 66  TYCNSVTGFGFGTINPSADCLTSNDKDYSNPDDMKISQ 104
            YCNS  GFG+G ++PSADCL S+D    N  D  +++
Sbjct: 416 AYCNSAEGFGYGVVHPSADCLYSHDDKSLNQTDQYLAE 453

[Close]

BLAST of Spo10762.1 vs. TAIR (Arabidopsis)
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein)

HSP 1 Score: 61.6 bits (148), Expect = 3.700e-10
Identity = 34/71 (47.89%), Postives = 44/71 (61.97%), Query Frame = 1


		  
Query: 17  VQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLH--KKDGTAETYCNSVTGF 76
           VQI  V Y NI GT+ SKEA+K  CS + PC+++ LSNI+LH   +DG     C +V G 
Sbjct: 333 VQIKDVKYNNIWGTSTSKEALKMQCSKTFPCQDVELSNINLHYVGRDGLVTALCENVGGS 392

Query: 77  GFGTINPSADC 86
             G I P A+C
Sbjct: 393 IRGKIVP-ANC 402

[Close]

BLAST of Spo10762.1 vs. TAIR (Arabidopsis)
Match: AT1G80170.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 61.2 bits (147), Expect = 4.800e-10
Identity = 31/78 (39.74%), Postives = 48/78 (61.54%), Query Frame = 1


		  
Query: 14  STAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKK--DGTAETYCNSV 73
           ++A+ I  + + ++ GT+ SKEA+K +CSDS PCRNI+L +IDL     DG  E++C   
Sbjct: 351 TSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEA 410

Query: 74  TGFGFGTINPSADCLTSN 90
            G   G + P   CL+ +
Sbjct: 411 YGSSSGQVYP-PPCLSDD 427

[Close]

BLAST of Spo10762.1 vs. TAIR (Arabidopsis)
Match: AT1G05660.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 61.2 bits (147), Expect = 4.800e-10
Identity = 33/73 (45.21%), Postives = 45/73 (61.64%), Query Frame = 1


		  
Query: 15  TAVQISQVMYRNITGTTKSKEAMKFACSDSVPCRNIVLSNIDLHKKDGT-AETYCNSVTG 74
           + V+ISQV Y+NI GT+ ++EAMK  CS S PC  I L +I L    GT A ++C +  G
Sbjct: 321 SGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTYNKGTPATSFCFNAVG 380

Query: 75  FGFGTINPSADCL 87
              G I P++ CL
Sbjct: 381 KSLGVIQPTS-CL 392

The following BLAST results are available for this feature:

BLAST of Spo10762.1 vs. NCBI nr
BLAST of Spo10762.1 vs. UniProtKB/TrEMBL
BLAST of Spo10762.1 vs. ExPASy Swiss-Prot
BLAST of Spo10762.1 vs. TAIR (Arabidopsis)

BLAST of Spo10762.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902156880\|gb\|KNA06047.1\|	4.8e-52	100.	hypothetical protein SOVF_1846...	[more]
gi\|731342707\|ref\|XP_010682531.1\|	3.0e-46	88.4	PREDICTED: probable polygalact...	[more]
gi\|728842873\|gb\|KHG22316.1\|	1.2e-34	65.5	hypothetical protein F383_0448...	[more]
gi\|823159084\|ref\|XP_012479372.1\|	1.5e-34	67.8	PREDICTED: pentatricopeptide r...	[more]
gi\|763763959\|gb\|KJB31213.1\|	1.5e-34	67.8	hypothetical protein B456_005G...	[more]

BLAST of Spo10762.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QFK6_SPIOL	3.3e-52	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8C189_BETVU	2.1e-46	88.4	Uncharacterized protein OS=Bet...	[more]
A0A0B0PG30_GOSAR	8.2e-35	65.5	Uncharacterized protein OS=Gos...	[more]
A0A0D2PUY8_GOSRA	1.1e-34	67.8	Uncharacterized protein OS=Gos...	[more]
A0A067JRM6_JATCU	8.5e-32	58.5	Uncharacterized protein OS=Jat...	[more]

BLAST of Spo10762.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
PGLR5_ARATH	8.5e-9	39.7	Probable polygalacturonase At1...	[more]
PGLR_ACTDE	2.7e-7	42.0	Polygalacturonase OS=Actinidia...	[more]
PGLR_PRUPE	4.7e-7	40.2	Polygalacturonase OS=Prunus pe...	[more]
PGLR2_CRYJA	8.0e-7	30.8	Polygalacturonase OS=Cryptomer...	[more]
PGLR2_JUNAS	8.0e-7	37.3	Polygalacturonase OS=Juniperus...	[more]

BLAST of Spo10762.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G23460.1	4.8e-26	52.6	Pectin lyase-like superfamily ...	[more]
AT1G70500.1	6.9e-25	53.0	Pectin lyase-like superfamily ...	[more]
AT2G33160.1	3.7e-10	47.8	glycoside hydrolase family 28 ...	[more]
AT1G80170.1	4.8e-10	39.7	Pectin lyase-like superfamily ...	[more]
AT1G05660.1	4.8e-10	45.2	Pectin lyase-like superfamily ...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 13..72 score: 5.5
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 14..87 score: 1.54
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 14..93 score: 2.8
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 14..115 score: 1.1
None	No IPR available	PANTHER	PTHR31375:SF29	PECTIN LYASE-LIKE PROTEIN-RELATED	coord: 14..115 score: 1.1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0071555	cell wall organization
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004650	polygalacturonase activity