Spo10348.1 (mRNA) | SpinachBase

Overview


Name	Spo10348.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	(Calmodulin 2) (EF hand calcium-binding family protein)
Location	chr3 : 9018202 .. 9018696 (-)
Sequence length	495

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTAGTGATAAGGAAGGAGTGAAGCTAGACGACGAGCAAATCGCAGAATTGAGGGAGATTTTCCGGTCATTTGATCGAAACAACGATGGAAAATTGACTCAATTAGAGTTAGGATCGTTGTTGCGATCATTAGGGTTAAAACCCAGCGAAGATCAGGTGGAAACCCTAATTCAAGCAGCAGATAAGAACAGCAATGGGATGATTGAATTTTCGGAGTTCATCTCCCTGGTATCCCCTGATTTAATCCCAACAAAATGCCCTTACAGTGAGGAACAGCTTAAGCAGTTGTTTAAGATGTTTGATAGGGATGGCAATGGGTTGATCACTGCGGCGGAGCTCGCCCATTCAATGGCGAAGCTCGGCCATGCTTTGACCGCCGAGGAGCTCACTGGTATGATCAAGGAGGCTGATACCGACGGCGATGGTTGTATCAACTATCAGGAATTCTCTCAGGCGATCACTTCGGCTGCCTTTGATAATTCTTGGACTTGA

mRNA sequence

ATGAGTAGTGATAAGGAAGGAGTGAAGCTAGACGACGAGCAAATCGCAGAATTGAGGGAGATTTTCCGGTCATTTGATCGAAACAACGATGGAAAATTGACTCAATTAGAGTTAGGATCGTTGTTGCGATCATTAGGGTTAAAACCCAGCGAAGATCAGGTGGAAACCCTAATTCAAGCAGCAGATAAGAACAGCAATGGGATGATTGAATTTTCGGAGTTCATCTCCCTGGTATCCCCTGATTTAATCCCAACAAAATGCCCTTACAGTGAGGAACAGCTTAAGCAGTTGTTTAAGATGTTTGATAGGGATGGCAATGGGTTGATCACTGCGGCGGAGCTCGCCCATTCAATGGCGAAGCTCGGCCATGCTTTGACCGCCGAGGAGCTCACTGGTATGATCAAGGAGGCTGATACCGACGGCGATGGTTGTATCAACTATCAGGAATTCTCTCAGGCGATCACTTCGGCTGCCTTTGATAATTCTTGGACTTGA

Coding sequence (CDS)

ATGAGTAGTGATAAGGAAGGAGTGAAGCTAGACGACGAGCAAATCGCAGAATTGAGGGAGATTTTCCGGTCATTTGATCGAAACAACGATGGAAAATTGACTCAATTAGAGTTAGGATCGTTGTTGCGATCATTAGGGTTAAAACCCAGCGAAGATCAGGTGGAAACCCTAATTCAAGCAGCAGATAAGAACAGCAATGGGATGATTGAATTTTCGGAGTTCATCTCCCTGGTATCCCCTGATTTAATCCCAACAAAATGCCCTTACAGTGAGGAACAGCTTAAGCAGTTGTTTAAGATGTTTGATAGGGATGGCAATGGGTTGATCACTGCGGCGGAGCTCGCCCATTCAATGGCGAAGCTCGGCCATGCTTTGACCGCCGAGGAGCTCACTGGTATGATCAAGGAGGCTGATACCGACGGCGATGGTTGTATCAACTATCAGGAATTCTCTCAGGCGATCACTTCGGCTGCCTTTGATAATTCTTGGACTTGA

Protein sequence

MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo10348	Spo10348	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo10348.1	Spo10348.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10348.1.CDS.1	Spo10348.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10348.1.exon.1	Spo10348.1.exon.1	exon

Homology

[Close]

BLAST of Spo10348.1 vs. NCBI nr
Match: gi|902226793|gb|KNA20489.1| (hypothetical protein SOVF_052090 [Spinacia oleracea])

HSP 1 Score: 324.7 bits (831), Expect = 9.200e-86
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 1


		  
Query: 1   MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA 60
           MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA
Sbjct: 121 MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA 180

Query: 61  ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK 120
           ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK
Sbjct: 181 ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK 240

Query: 121 LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT 165
           LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT
Sbjct: 241 LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT 284

[Close]

BLAST of Spo10348.1 vs. NCBI nr
Match: gi|731320990|ref|XP_010671108.1| (PREDICTED: probable calcium-binding protein CML18 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 312.0 bits (798), Expect = 6.200e-82
Identity = 155/163 (95.09%), Postives = 162/163 (99.39%), Query Frame = 1


		  
Query: 1   MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA 60
           MSSDK+GVKLDDEQIAELREIFR+FDRNNDGKLTQLELGSLLRSLGLKPSEDQ+ETLIQA
Sbjct: 1   MSSDKDGVKLDDEQIAELREIFRTFDRNNDGKLTQLELGSLLRSLGLKPSEDQLETLIQA 60

Query: 61  ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK 120
           ADKN+NGMIEFSEFISLVSPDLIP KCPYSE+QLKQLFKMFDRDGNGLITAAELAHSMAK
Sbjct: 61  ADKNNNGMIEFSEFISLVSPDLIPAKCPYSEDQLKQLFKMFDRDGNGLITAAELAHSMAK 120

Query: 121 LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           LGHALTAEELTGMIKEADTDGDGCI++QEFSQAITSAAFDNSW
Sbjct: 121 LGHALTAEELTGMIKEADTDGDGCISFQEFSQAITSAAFDNSW 163

[Close]

BLAST of Spo10348.1 vs. NCBI nr
Match: gi|802585060|ref|XP_012070221.1| (PREDICTED: probable calcium-binding protein CML18 [Jatropha curcas])

HSP 1 Score: 284.6 bits (727), Expect = 1.100e-73
Identity = 138/162 (85.19%), Postives = 153/162 (94.44%), Query Frame = 1


		  
Query: 3   SDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAAD 62
           S K+ VKLDDEQ+AELREIFRSFDRN DG LTQLELGSLLRSLGLKPSEDQ+E LIQ AD
Sbjct: 2   SGKDPVKLDDEQLAELREIFRSFDRNKDGSLTQLELGSLLRSLGLKPSEDQLEALIQKAD 61

Query: 63  KNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLG 122
           KNSNG+IEFSEF++LV PDLIP+KCPY+E+QLK++F+MFDRDGNG IT AELAHSMAKLG
Sbjct: 62  KNSNGLIEFSEFVALVEPDLIPSKCPYTEDQLKKIFRMFDRDGNGYITPAELAHSMAKLG 121

Query: 123 HALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT 165
           HALTAEELTGMIKEADTDGDGCIN+QEF+QAITSAAFDNSW+
Sbjct: 122 HALTAEELTGMIKEADTDGDGCINFQEFAQAITSAAFDNSWS 163

[Close]

BLAST of Spo10348.1 vs. NCBI nr
Match: gi|702251619|ref|XP_010063341.1| (PREDICTED: probable calcium-binding protein CML18 [Eucalyptus grandis])

HSP 1 Score: 281.2 bits (718), Expect = 1.200e-72
Identity = 136/159 (85.53%), Postives = 148/159 (93.08%), Query Frame = 1


		  
Query: 5   KEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKN 64
           KE VKLDDEQ+AELREIFRSFDRNNDG LTQLELGSLLRSLGLKPS DQ+E LI  AD+N
Sbjct: 9   KESVKLDDEQLAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLEALIHKADRN 68

Query: 65  SNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHA 124
           SNG++EFSEF++LV PDLIP KCPY+E+QLK++F MFDRDGNG ITAAELAHSMAKLGHA
Sbjct: 69  SNGLVEFSEFVALVEPDLIPAKCPYTEDQLKKIFHMFDRDGNGYITAAELAHSMAKLGHA 128

Query: 125 LTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           LTAEELTGMIKEADTDGDGCIN+QEF QAITSAAFDNSW
Sbjct: 129 LTAEELTGMIKEADTDGDGCINFQEFVQAITSAAFDNSW 167

[Close]

BLAST of Spo10348.1 vs. NCBI nr
Match: gi|659095574|ref|XP_008448654.1| (PREDICTED: probable calcium-binding protein CML18 [Cucumis melo])

HSP 1 Score: 278.5 bits (711), Expect = 7.600e-72
Identity = 136/161 (84.47%), Postives = 149/161 (92.55%), Query Frame = 1


		  
Query: 3   SDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAAD 62
           SDK+ +KLDDEQIAELREIFRSFDRNNDG LTQLELGSLLRSLGLKPS DQ+E LI  AD
Sbjct: 2   SDKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLENLIMKAD 61

Query: 63  KNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLG 122
            NSNG++EFSEF++LV P+L+  KCPY+EEQLKQLF+MFDRDGNG ITAAELAHSMAKLG
Sbjct: 62  TNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLG 121

Query: 123 HALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           HALTAEELTGMI+EADTDGDG IN+QEFSQAITSAAFDNSW
Sbjct: 122 HALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSW 162

[Close]

BLAST of Spo10348.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RNK1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_052090 PE=4 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 6.400e-86
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 1


		  
Query: 1   MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA 60
           MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA
Sbjct: 121 MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA 180

Query: 61  ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK 120
           ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK
Sbjct: 181 ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK 240

Query: 121 LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT 165
           LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT
Sbjct: 241 LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT 284

[Close]

BLAST of Spo10348.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CSW0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g049760 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 4.300e-82
Identity = 155/163 (95.09%), Postives = 162/163 (99.39%), Query Frame = 1


		  
Query: 1   MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA 60
           MSSDK+GVKLDDEQIAELREIFR+FDRNNDGKLTQLELGSLLRSLGLKPSEDQ+ETLIQA
Sbjct: 1   MSSDKDGVKLDDEQIAELREIFRTFDRNNDGKLTQLELGSLLRSLGLKPSEDQLETLIQA 60

Query: 61  ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK 120
           ADKN+NGMIEFSEFISLVSPDLIP KCPYSE+QLKQLFKMFDRDGNGLITAAELAHSMAK
Sbjct: 61  ADKNNNGMIEFSEFISLVSPDLIPAKCPYSEDQLKQLFKMFDRDGNGLITAAELAHSMAK 120

Query: 121 LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           LGHALTAEELTGMIKEADTDGDGCI++QEFSQAITSAAFDNSW
Sbjct: 121 LGHALTAEELTGMIKEADTDGDGCISFQEFSQAITSAAFDNSW 163

[Close]

BLAST of Spo10348.1 vs. UniProtKB/TrEMBL
Match: A0A067L4T3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02535 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 7.400e-74
Identity = 138/162 (85.19%), Postives = 153/162 (94.44%), Query Frame = 1


		  
Query: 3   SDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAAD 62
           S K+ VKLDDEQ+AELREIFRSFDRN DG LTQLELGSLLRSLGLKPSEDQ+E LIQ AD
Sbjct: 2   SGKDPVKLDDEQLAELREIFRSFDRNKDGSLTQLELGSLLRSLGLKPSEDQLEALIQKAD 61

Query: 63  KNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLG 122
           KNSNG+IEFSEF++LV PDLIP+KCPY+E+QLK++F+MFDRDGNG IT AELAHSMAKLG
Sbjct: 62  KNSNGLIEFSEFVALVEPDLIPSKCPYTEDQLKKIFRMFDRDGNGYITPAELAHSMAKLG 121

Query: 123 HALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT 165
           HALTAEELTGMIKEADTDGDGCIN+QEF+QAITSAAFDNSW+
Sbjct: 122 HALTAEELTGMIKEADTDGDGCINFQEFAQAITSAAFDNSWS 163

[Close]

BLAST of Spo10348.1 vs. UniProtKB/TrEMBL
Match: A0A068VFR9_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00010775001 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 6.900e-72
Identity = 137/162 (84.57%), Postives = 152/162 (93.83%), Query Frame = 1


		  
Query: 3   SDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAAD 62
           S KE V LDDEQ+AELREIFRSFDRNNDG LTQLELGSLLRSLGLKPS DQ+ETLIQ AD
Sbjct: 2   SSKEPVSLDDEQLAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLETLIQKAD 61

Query: 63  KNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLG 122
           +N+NG++EFSEFI+LV+P+L+P K PY+EEQLKQLF+MFDRDGNG ITAAELAHSMAKLG
Sbjct: 62  RNNNGLVEFSEFIALVAPELLPAKSPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLG 121

Query: 123 HALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSWT 165
           HALTAEELTGMIKEADTDGDG I++QEFSQAITSAAFDNSW+
Sbjct: 122 HALTAEELTGMIKEADTDGDGRISFQEFSQAITSAAFDNSWS 163

[Close]

BLAST of Spo10348.1 vs. UniProtKB/TrEMBL
Match: B9MU63_POPTR (Calmodulin family protein OS=Populus trichocarpa GN=POPTR_0001s02050g PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 6.900e-72
Identity = 139/163 (85.28%), Postives = 150/163 (92.02%), Query Frame = 1


		  
Query: 1   MSSDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQA 60
           MS+ K  VKLDDEQIAELREIFRSFDRNNDG LTQLELGSLLRSLGLKP  DQ+ETLIQ 
Sbjct: 1   MSNKKPAVKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPRPDQLETLIQK 60

Query: 61  ADKNSNGMIEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAK 120
           AD NSNG+IEFSEF++LV+P+L+P K PYSEEQLK LFKMFDRDGNG ITAAELAHSMAK
Sbjct: 61  ADTNSNGLIEFSEFVALVAPELLPEKSPYSEEQLKHLFKMFDRDGNGFITAAELAHSMAK 120

Query: 121 LGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           LGHALTAEELTGMIKEADTDGDG I+++EFSQAITSAAFDNSW
Sbjct: 121 LGHALTAEELTGMIKEADTDGDGRISFEEFSQAITSAAFDNSW 163

[Close]

BLAST of Spo10348.1 vs. ExPASy Swiss-Prot
Match: CML18_ARATH (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana GN=CML18 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 2.500e-67
Identity = 123/155 (79.35%), Postives = 144/155 (92.90%), Query Frame = 1


		  
Query: 9   KLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGM 68
           KL DEQ+AELREIFRSFD+N DG LT+LELGSLLRSLGLKPS+DQ++TLIQ AD+N+NG+
Sbjct: 12  KLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGL 71

Query: 69  IEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAE 128
           +EFSEF++LV PDL+  KCPY+++QLK +F+MFDRDGNG ITAAELAHSMAKLGHALTAE
Sbjct: 72  VEFSEFVALVEPDLV--KCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAE 131

Query: 129 ELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           ELTGMIKEAD DGDGCI++QEF QAITSAAFDN+W
Sbjct: 132 ELTGMIKEADRDGDGCIDFQEFVQAITSAAFDNAW 164

[Close]

BLAST of Spo10348.1 vs. ExPASy Swiss-Prot
Match: CML11_ORYSJ (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica GN=CML11 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 7.100e-62
Identity = 115/152 (75.66%), Postives = 138/152 (90.79%), Query Frame = 1


		  
Query: 10  LDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGMI 69
           L D+Q+ ELREIFRSFDRN DG LTQLELGSLLRSLGLKPS D++++LIQ AD NSNG+I
Sbjct: 57  LGDDQLGELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGLI 116

Query: 70  EFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAEE 129
           EFSEF++LV+P+L+  + PYSE+Q+++LF +FDRDGNG ITAAELAHSMAKLGHALT +E
Sbjct: 117 EFSEFVALVAPELLYDRAPYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKLGHALTVKE 176

Query: 130 LTGMIKEADTDGDGCINYQEFSQAITSAAFDN 162
           LTGMIKEADTDGDG I++QEFS+AIT+AAFDN
Sbjct: 177 LTGMIKEADTDGDGRISFQEFSRAITAAAFDN 208

[Close]

BLAST of Spo10348.1 vs. ExPASy Swiss-Prot
Match: CML17_ARATH (Probable calcium-binding protein CML17 OS=Arabidopsis thaliana GN=CML17 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 8.700e-60
Identity = 119/164 (72.56%), Postives = 137/164 (83.54%), Query Frame = 1


		  
Query: 3   SDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAAD 62
           S K   KLD+EQI ELREIFRSFDRN DG LTQLELGSLLR+LG+KPS DQ ETLI  AD
Sbjct: 2   SHKVSKKLDEEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKAD 61

Query: 63  KNSNGMIEFSEFISLVSPDLI-PTK--CPYSEEQLKQLFKMFDRDGNGLITAAELAHSMA 122
             SNG++EF EF++LVSP+L+ P K   PY+EEQL +LF++FD DGNG ITAAELAHSMA
Sbjct: 62  TKSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMA 121

Query: 123 KLGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           KLGHALT  ELTGMIKEAD+DGDG IN+QEF++AI SAAFD+ W
Sbjct: 122 KLGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSAAFDDIW 165

[Close]

BLAST of Spo10348.1 vs. ExPASy Swiss-Prot
Match: CML14_ORYSJ (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica GN=CML14 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 3.200e-38
Identity = 78/152 (51.32%), Postives = 106/152 (69.74%), Query Frame = 1


		  
Query: 9   KLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGM 68
           +L   Q+ +LRE+FR FD N DG LTQLEL +LLRSLGL+P+ D+V  L+   D N NG 
Sbjct: 17  QLRGSQLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGS 76

Query: 69  IEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAE 128
           +EF E  + ++P L        + QL ++F+ FDRDGNG I+AAELA SMA+LG  LT E
Sbjct: 77  VEFDELAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFE 136

Query: 129 ELTGMIKEADTDGDGCINYQEFSQAITSAAFD 161
           ELT M+++ADTDGDG I+++EF+  +  +A D
Sbjct: 137 ELTRMMRDADTDGDGVISFKEFAAVMAKSALD 168

[Close]

BLAST of Spo10348.1 vs. ExPASy Swiss-Prot
Match: CML16_ARATH (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana GN=CML16 PE=2 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 2.100e-37
Identity = 78/148 (52.70%), Postives = 105/148 (70.95%), Query Frame = 1


		  
Query: 13  EQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGMIEFS 72
           +QI +L++IF  FD + DG LTQLEL +LLRSLG+KP  DQ+  L+   D+N NG +EF 
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 73  EFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAEELTG 132
           E +  + PD I  +   ++EQL ++F+ FDRDGNG ITAAELA SMAK+GH LT  ELT 
Sbjct: 68  ELVVAILPD-INEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTE 127

Query: 133 MIKEADTDGDGCINYQEFSQAITSAAFD 161
           M+ EAD++GDG I++ EFS  +  +A D
Sbjct: 128 MMTEADSNGDGVISFNEFSHIMAKSAAD 154

[Close]

BLAST of Spo10348.1 vs. TAIR (Arabidopsis)
Match: AT3G03000.1 (EF hand calcium-binding protein family)

HSP 1 Score: 256.1 bits (653), Expect = 1.400e-68
Identity = 123/155 (79.35%), Postives = 144/155 (92.90%), Query Frame = 1


		  
Query: 9   KLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGM 68
           KL DEQ+AELREIFRSFD+N DG LT+LELGSLLRSLGLKPS+DQ++TLIQ AD+N+NG+
Sbjct: 12  KLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGL 71

Query: 69  IEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAE 128
           +EFSEF++LV PDL+  KCPY+++QLK +F+MFDRDGNG ITAAELAHSMAKLGHALTAE
Sbjct: 72  VEFSEFVALVEPDLV--KCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAE 131

Query: 129 ELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           ELTGMIKEAD DGDGCI++QEF QAITSAAFDN+W
Sbjct: 132 ELTGMIKEADRDGDGCIDFQEFVQAITSAAFDNAW 164

[Close]

BLAST of Spo10348.1 vs. TAIR (Arabidopsis)
Match: AT1G32250.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 231.1 bits (588), Expect = 4.900e-61
Identity = 119/164 (72.56%), Postives = 137/164 (83.54%), Query Frame = 1


		  
Query: 3   SDKEGVKLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAAD 62
           S K   KLD+EQI ELREIFRSFDRN DG LTQLELGSLLR+LG+KPS DQ ETLI  AD
Sbjct: 2   SHKVSKKLDEEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKAD 61

Query: 63  KNSNGMIEFSEFISLVSPDLI-PTK--CPYSEEQLKQLFKMFDRDGNGLITAAELAHSMA 122
             SNG++EF EF++LVSP+L+ P K   PY+EEQL +LF++FD DGNG ITAAELAHSMA
Sbjct: 62  TKSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMA 121

Query: 123 KLGHALTAEELTGMIKEADTDGDGCINYQEFSQAITSAAFDNSW 164
           KLGHALT  ELTGMIKEAD+DGDG IN+QEF++AI SAAFD+ W
Sbjct: 122 KLGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSAAFDDIW 165

[Close]

BLAST of Spo10348.1 vs. TAIR (Arabidopsis)
Match: AT3G25600.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 156.8 bits (395), Expect = 1.200e-38
Identity = 78/148 (52.70%), Postives = 105/148 (70.95%), Query Frame = 1


		  
Query: 13  EQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGMIEFS 72
           +QI +L++IF  FD + DG LTQLEL +LLRSLG+KP  DQ+  L+   D+N NG +EF 
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 73  EFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAEELTG 132
           E +  + PD I  +   ++EQL ++F+ FDRDGNG ITAAELA SMAK+GH LT  ELT 
Sbjct: 68  ELVVAILPD-INEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTE 127

Query: 133 MIKEADTDGDGCINYQEFSQAITSAAFD 161
           M+ EAD++GDG I++ EFS  +  +A D
Sbjct: 128 MMTEADSNGDGVISFNEFSHIMAKSAAD 154

[Close]

BLAST of Spo10348.1 vs. TAIR (Arabidopsis)
Match: AT1G18530.1 (EF hand calcium-binding protein family)

HSP 1 Score: 156.0 bits (393), Expect = 2.000e-38
Identity = 79/149 (53.02%), Postives = 106/149 (71.14%), Query Frame = 1


		  
Query: 12  DEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGMIEF 71
           ++QI +L++IF  FD + DG LT LEL +LLRSLGLKPS DQ+  L+ + D N NG +EF
Sbjct: 2   EDQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEF 61

Query: 72  SEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAEELT 131
            E +  + PDL   +   + EQL ++FK FDRDGNG I+AAELA +MAK+G  LT +ELT
Sbjct: 62  DELVGTILPDL-NEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELT 121

Query: 132 GMIKEADTDGDGCINYQEFSQAITSAAFD 161
            MIKEADT+GDG I++ EF+  +  +A D
Sbjct: 122 EMIKEADTNGDGVISFGEFASIMAKSAVD 149

[Close]

BLAST of Spo10348.1 vs. TAIR (Arabidopsis)
Match: AT3G56800.1 (calmodulin 3)

HSP 1 Score: 141.0 bits (354), Expect = 6.700e-34
Identity = 70/142 (49.30%), Postives = 100/142 (70.42%), Query Frame = 1


		  
Query: 9   KLDDEQIAELREIFRSFDRNNDGKLTQLELGSLLRSLGLKPSEDQVETLIQAADKNSNGM 68
           +L D+QI+E +E F  FD++ DG +T  ELG+++RSLG  P+E +++ +I   D + NG 
Sbjct: 4   QLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGT 63

Query: 69  IEFSEFISLVSPDLIPTKCPYSEEQLKQLFKMFDRDGNGLITAAELAHSMAKLGHALTAE 128
           I+F EF++L++  +  T    SEE+LK+ F++FD+D NG I+AAEL H M  LG  LT E
Sbjct: 64  IDFPEFLNLMARKMKDTD---SEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 123

Query: 129 ELTGMIKEADTDGDGCINYQEF 151
           E+  MIKEAD DGDG INY+EF
Sbjct: 124 EVDEMIKEADVDGDGQINYEEF 142

The following BLAST results are available for this feature:

BLAST of Spo10348.1 vs. NCBI nr
BLAST of Spo10348.1 vs. UniProtKB/TrEMBL
BLAST of Spo10348.1 vs. ExPASy Swiss-Prot
BLAST of Spo10348.1 vs. TAIR (Arabidopsis)

BLAST of Spo10348.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902226793\|gb\|KNA20489.1\|	9.2e-86	100.	hypothetical protein SOVF_0520...	[more]
gi\|731320990\|ref\|XP_010671108.1\|	6.2e-82	95.0	PREDICTED: probable calcium-bi...	[more]
gi\|802585060\|ref\|XP_012070221.1\|	1.1e-73	85.1	PREDICTED: probable calcium-bi...	[more]
gi\|702251619\|ref\|XP_010063341.1\|	1.2e-72	85.5	PREDICTED: probable calcium-bi...	[more]
gi\|659095574\|ref\|XP_008448654.1\|	7.6e-72	84.4	PREDICTED: probable calcium-bi...	[more]

BLAST of Spo10348.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RNK1_SPIOL	6.4e-86	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CSW0_BETVU	4.3e-82	95.0	Uncharacterized protein OS=Bet...	[more]
A0A067L4T3_JATCU	7.4e-74	85.1	Uncharacterized protein OS=Jat...	[more]
A0A068VFR9_COFCA	6.9e-72	84.5	Uncharacterized protein OS=Cof...	[more]
B9MU63_POPTR	6.9e-72	85.2	Calmodulin family protein OS=P...	[more]

BLAST of Spo10348.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
CML18_ARATH	2.5e-67	79.3	Probable calcium-binding prote...	[more]
CML11_ORYSJ	7.1e-62	75.6	Probable calcium-binding prote...	[more]
CML17_ARATH	8.7e-60	72.5	Probable calcium-binding prote...	[more]
CML14_ORYSJ	3.2e-38	51.3	Probable calcium-binding prote...	[more]
CML16_ARATH	2.1e-37	52.7	Probable calcium-binding prote...	[more]

BLAST of Spo10348.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G03000.1	1.4e-68	79.3	EF hand calcium-binding protei...	[more]
AT1G32250.1	4.9e-61	72.5	Calcium-binding EF-hand family...	[more]
AT3G25600.1	1.2e-38	52.7	Calcium-binding EF-hand family...	[more]
AT1G18530.1	2.0e-38	53.0	EF hand calcium-binding protei...	[more]
AT3G56800.1	6.7e-34	49.3	calmodulin 3	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 17..78 score: 1.0E-14coord: 91..154 score: 3.1
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 53..81 score: 0.26coord: 129..157 score: 5.5E-5coord: 17..45 score: 1.3E-5coord: 93..121 score: 9.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 125..160 score: 12.868coord: 13..48 score: 15.518coord: 49..84 score: 11.445coord: 89..124 score: 15
IPR011992	EF-hand domain pair	GENE3D	1.10.238.10		coord: 105..156 score: 1.9E-23coord: 12..104 score: 2.5
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 9..155 score: 4.43
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 62..74 score: -coord: 102..114 score: -coord: 138..150 score: -coord: 26..38 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..164 score: 2.8E
None	No IPR available	PANTHER	PTHR10891:SF290	CALCIUM-BINDING PROTEIN CML18-RELATED	coord: 5..164 score: 2.8E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)] activity