Spo08789.1 (mRNA) | SpinachBase

Overview


Name	Spo08789.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Protein-L-isoaspartate O-methyltransferase (2.1.1.77)
Location	chr1 : 43375898 .. 43379948 (+)
Sequence length	1319

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGGATGATCCAGAATTCCAGAAATAGGAATAAAGCAGAGTAGCAGACCCAGAAAATTCCTTTGACTTTAAAAAAAAAAAAAAAAGGAAATAAGAAAAGAGATGCCATTACTTCCGTCTCCTATCCTGGCGTATCGTGCGCCTTATTTCAAGCACTTGACAAACTTCTCCTCCATTTTGCCACACCACCATCTTCCTCCTCTCCTGCTTCCACGTGTCCCCACCGCTTTCCTCCGGACTTTCTCTCTCTTTAACCCTTGCTCTCCTCTCTCCACCACGGGCAACTCTCTTCTCTCTCGCATGGAGGTTCTGTCTCTTAATTTCTTGCTTTTTTATTTATTCATTTGGGGTTATTTTAATTTATTTGTTTAAATTGTGTTTTTGTTCCTAGTTGTTTCAATATCCAGCTTGTTGATGGTAATATGGGTTCTTTGTAATTTGATCAAAAGTATCCACAAATAAATTGTTTATTTTGCTCTTAGTTTGACTCTTTTGATGATGATTCTGTCAAATTTTTGTTTTTAGGTAAGAAATGTGATCATAGTGGTTTGATTGTGTGGATATGGGTTGATTTTAATTACTCATAAGATGTTAATTTTTATTATTATGGTGGAAATGATAACTACCCCTTGGTTATTGACATTTGACAATTAAAACAAGTGCCTGACTAGGCTATTTTGTAAAAATTTCCATGCTTTTAGCCCAAATTGAAGTGTTCAAATCTCCATACCCATATTGGAGTGTCAAGGAGGTATTTGAGGTGAAATGAAGATGTGAATTGGTCTACATCACTGCTCTAAGTGGCTTCATATGCACCAAAAGGCTACTTAGATGCTGTATTTTTAGAAAAAAATAATGGATGTCCTGTAATTTGTGAAGGACTTGAATGTATTCAGTGGCTAATCATTCAAAGTTTATTCTTTTTACTGTTTGCAAAAGGTAGAGCTTTCAACAGATGATCTGAAAGCTTGGAAGGTTGGTGATTTTGTGGTTTCCTACAGATTTTTGTTGCTTCTAATAAGATTCTTGATTAGCAAGAGGAGATTTAGGTGCTTTGTTTGTCGTAATTGAGCTATTATGATTATTAGAGCTTTGAATAAACTCAGAGTAATGTTGATTAACCAAAAGTATTGAAGCTGGTATGTTTATGACAGAGTGTTGTTTTGGAATCTTTTGTTGGGGGTATGACTTCAAACTATCAGCATATCAACTGATGGATTTACCTGGGGTATTTCAGTCACTCAGACTTGGTTAATTTATGAAAATTTTACGTATTTTTGAGCCAGATGGAAATGCCAAGTGTCCATACTAATGTCGATTTGTCTAGCATCCAACACTAGGTTACTTGACGTGATATGAAGAGTCTGAGACTCGAGTTACATTGTTTGCATTGGAAATTGGCGCAAGAAGTACTATACTTGGACCAGCTACAAAGAGTACCTTTGTGTTAGTATATACATATGTTTTATTCTGATGATTTGGATTTTGGAGACAGCATAGGCATTTACAGATTTCCTACTTGGCTGCCTCTTAGAAAGTTTCTTTCAACTAGTTGCTAGTTATTTGCTTGTTGAGTTTGGTTAACCTTTGACTAATGTCCTTTCTTTGTTTGATGTTTTTAGTGTAACATTTATCTAGTTTTTGTGATTTATCTGCAGAAACCCTTTTCTGGTTCTGGAGGTGGCATGGCCAAAAGCAAAGCTATGGTGGAACAGTTGCGGCGTTGTGGAGTTTTTCACTCTGAGAAGGTTGCTGAAGTGATGGAAACCATTGATAGGGGTTTGTTTGTACCTGCTGGGACCCCGGCTTATATGGATAGCCCCATGCCAATAGGCCATAATGCCACCATATCAGCTCCTCATATGCATGCAATGTGCCTTGATCTTTTAAACGATAACTTGCAGCCTGGCATGCATGCTTTAGATGTTGGATCAGGTGATTTTCTTTATCTATGCTTGTTTATGGAGTCTCTCTTATTGCTATTTCAGTATATACTTTTTCAGTACAAGGTCATGAATGACAGGAATGATACTGACATGCTTGTGTGTCTGTTCTGCATGCAATGCCTCGTATGGTTTTCCATGCAATGCAATCTTCATGAGAAAGCAACATAATTTCTTTCATTTTCTCTACTACTCACTACTACGATGGTCATGGTTACACCTAGATTCCTAGAGATGTGGGTATGATTCATAATGATAATGAAGCACGGCAAATGCTAGCTATTAAAGATGCATTATTACGGACAATTGGACATTCCCTGAGACTTTTGACTAAGAATTACAAACTCATTCACTACTATTGTCTCAATTGATTACTTCATACTAGTCCTGCAGTTTTTAGGATCTTGTTAACATTTCTGCATTTTCTATATTTCTCCCATGCCCCTTGGAAGTAGACTTTGTAGAGACGAGAGAGAATTGGTGGTCACCAATTTTTTAATAGAAAGTGGTGTCATCATTGGTGTGGGAGTAGATGCATTAATGTGACAACTTTTATGGATATTTTTCTTCTGTTAAACCTTCCAAAGCCTTGAAGGCTTCTGCTATGCCTTTTTCATGTGACTTATTTATTTTCGCCTGAGAGAGCTTTTTCACAGAAGAGTAAAAGGAATAATTCTGGTTTCCCAACTTCTTCACCTTTTCCCAGTCTAATCACCCAAAGAATTCCTATGAGGGAAGGGAACAAGTTTTAATTGCGTCGAATCCATGCTCTCCGCAACCAGGAGGGCAATAATGTTTGTGTGATTGTTAAACTAAGGGTGCGTTCTATTCAACTGATTTTCACTTTTTTCTGAACTTATATTATCTGAACTTAACTGAACTTATTAAAACTTATCAAAACTTATTTTAGTTATAAGTTGTACTTGGCCCACCCTTATTTTTCCTGAACATATCTTAACTGAACTTATCTGAACTTAACTGAACTTATCTTAACTTATTTTCCCTGAAATAAGTGGAAATAAGGTGAACAGAACAGGGCCTAAGACGTGATGAAAGATTTTTTTTGCTTATGAAAGCAATTAAGTTGAATGAGCAAAACTAGGCTCAGTACTGATACCAAACAGCATATGATGAAGGGAAACCTTAGTGTTGGCCTATTTCAACTTTATTCATAAGAAATTGATTCAACAATATCAATGCCAAGTTATTAGCCTCACACCCTGTGCAAGTGAGTGACCTCGTTGGTATGGACTGCTTTACTCGCAGGCACAGGGTACTTGACTGCATGTTTTGCTGTGATGGTTGGATCCGAAGGTCGTGCAGTGGGTATAGAGCACATTCCTGAACTGGTGACTTGGTCAATTGAAAACATCAAAAAGAGTAAAGCTGCTTCTTTGCTAGAAGAGGGCTCCTTGTCAGTTCATGTTGGTGGTAAGATCTCAAACTGCTCACACATCTCCCTACAATTATACAATGTATGTTTCTTTCTTGCTTATTTATTCATATGTTACTCGTTCCGTGATATTATTGGTGACAGATGGAAGGCAAGGATATCCTGAGGATGCACCATATGATGCAATCCATGTTGGTGCAGCAGCACCAGAAATCCCAAAGGCGTTGATTGAGCAGCTGAAGCCCGGTGGACGGTTGGTGATACCAGTAGGAACCTTCTTCCAGGACTTGCAGGTTATTGACAAGAAGCAGGATGGCTCTCTTTCTGTCAGAACTGAAACTTCTGTGAGATATGTTCCACTGACTAGTCGGGAAGAGCAGTTGCGGGGTTAATTGAACTGCCTTTATCTATGTTCTATTTTGTGTATATTTAATGTTTTTTATTTTGTTTTTCATTTGCTGCGCGTTATCCATGAATTGGAAGCTTATAGCAGCATGTATTTTCCTCCCTTTTGTAAATATTTGACATCGTGGATGTAAAGTGTATCAAAGTTGGACACTGTACTTCATCAAAATGACGCTCCTTGTACTAGGCTAAAGTAAGCAGCAAGTGGATTGATTCGTTAAATTGTGGATGATACTAGAGGTACATAAAACAATGGCATTGGTTTTGAGAAATCAATTAGTTTTTTTTGCTTATTTTAGACAAATTATAC

mRNA sequence

AATGGATGATCCAGAATTCCAGAAATAGGAATAAAGCAGAGTAGCAGACCCAGAAAATTCCTTTGACTTTAAAAAAAAAAAAAAAAGGAAATAAGAAAAGAGATGCCATTACTTCCGTCTCCTATCCTGGCGTATCGTGCGCCTTATTTCAAGCACTTGACAAACTTCTCCTCCATTTTGCCACACCACCATCTTCCTCCTCTCCTGCTTCCACGTGTCCCCACCGCTTTCCTCCGGACTTTCTCTCTCTTTAACCCTTGCTCTCCTCTCTCCACCACGGGCAACTCTCTTCTCTCTCGCATGGAGAAACCCTTTTCTGGTTCTGGAGGTGGCATGGCCAAAAGCAAAGCTATGGTGGAACAGTTGCGGCGTTGTGGAGTTTTTCACTCTGAGAAGGTTGCTGAAGTGATGGAAACCATTGATAGGGGTTTGTTTGTACCTGCTGGGACCCCGGCTTATATGGATAGCCCCATGCCAATAGGCCATAATGCCACCATATCAGCTCCTCATATGCATGCAATGTGCCTTGATCTTTTAAACGATAACTTGCAGCCTGGCATGCATGCTTTAGATGTTGGATCAGGCACAGGGTACTTGACTGCATGTTTTGCTGTGATGGTTGGATCCGAAGGTCGTGCAGTGGGTATAGAGCACATTCCTGAACTGGTGACTTGGTCAATTGAAAACATCAAAAAGAGTAAAGCTGCTTCTTTGCTAGAAGAGGGCTCCTTGTCAGTTCATGTTGGTGATGGAAGGCAAGGATATCCTGAGGATGCACCATATGATGCAATCCATGTTGGTGCAGCAGCACCAGAAATCCCAAAGGCGTTGATTGAGCAGCTGAAGCCCGGTGGACGGTTGGTGATACCAGTAGGAACCTTCTTCCAGGACTTGCAGGTTATTGACAAGAAGCAGGATGGCTCTCTTTCTGTCAGAACTGAAACTTCTGTGAGATATGTTCCACTGACTAGTCGGGAAGAGCAGTTGCGGGGTTAATTGAACTGCCTTTATCTATGTTCTATTTTGTGTATATTTAATGTTTTTTATTTTGTTTTTCATTTGCTGCGCGTTATCCATGAATTGGAAGCTTATAGCAGCATGTATTTTCCTCCCTTTTGTAAATATTTGACATCGTGGATGTAAAGTGTATCAAAGTTGGACACTGTACTTCATCAAAATGACGCTCCTTGTACTAGGCTAAAGTAAGCAGCAAGTGGATTGATTCGTTAAATTGTGGATGATACTAGAGGTACATAAAACAATGGCATTGGTTTTGAGAAATCAATTAGTTTTTTTTGCTTATTTTAGACAAATTATAC

Coding sequence (CDS)

ATGCCATTACTTCCGTCTCCTATCCTGGCGTATCGTGCGCCTTATTTCAAGCACTTGACAAACTTCTCCTCCATTTTGCCACACCACCATCTTCCTCCTCTCCTGCTTCCACGTGTCCCCACCGCTTTCCTCCGGACTTTCTCTCTCTTTAACCCTTGCTCTCCTCTCTCCACCACGGGCAACTCTCTTCTCTCTCGCATGGAGAAACCCTTTTCTGGTTCTGGAGGTGGCATGGCCAAAAGCAAAGCTATGGTGGAACAGTTGCGGCGTTGTGGAGTTTTTCACTCTGAGAAGGTTGCTGAAGTGATGGAAACCATTGATAGGGGTTTGTTTGTACCTGCTGGGACCCCGGCTTATATGGATAGCCCCATGCCAATAGGCCATAATGCCACCATATCAGCTCCTCATATGCATGCAATGTGCCTTGATCTTTTAAACGATAACTTGCAGCCTGGCATGCATGCTTTAGATGTTGGATCAGGCACAGGGTACTTGACTGCATGTTTTGCTGTGATGGTTGGATCCGAAGGTCGTGCAGTGGGTATAGAGCACATTCCTGAACTGGTGACTTGGTCAATTGAAAACATCAAAAAGAGTAAAGCTGCTTCTTTGCTAGAAGAGGGCTCCTTGTCAGTTCATGTTGGTGATGGAAGGCAAGGATATCCTGAGGATGCACCATATGATGCAATCCATGTTGGTGCAGCAGCACCAGAAATCCCAAAGGCGTTGATTGAGCAGCTGAAGCCCGGTGGACGGTTGGTGATACCAGTAGGAACCTTCTTCCAGGACTTGCAGGTTATTGACAAGAAGCAGGATGGCTCTCTTTCTGTCAGAACTGAAACTTCTGTGAGATATGTTCCACTGACTAGTCGGGAAGAGCAGTTGCGGGGTTAA

Protein sequence

MPLLPSPILAYRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTGNSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo08789	Spo08789	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo08789.1	Spo08789.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08789.1.exon.1	Spo08789.1.exon.1	exon
Spo08789.1.exon.2	Spo08789.1.exon.2	exon
Spo08789.1.exon.3	Spo08789.1.exon.3	exon
Spo08789.1.exon.4	Spo08789.1.exon.4	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08789.1.utr5p.1	Spo08789.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08789.1.CDS.1	Spo08789.1.CDS.1	CDS
Spo08789.1.CDS.2	Spo08789.1.CDS.2	CDS
Spo08789.1.CDS.3	Spo08789.1.CDS.3	CDS
Spo08789.1.CDS.4	Spo08789.1.CDS.4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08789.1.utr3p.1	Spo08789.1.utr3p.1	three_prime_UTR

Homology

[Close]

BLAST of Spo08789.1 vs. NCBI nr
Match: gi|902169091|gb|KNA07602.1| (hypothetical protein SOVF_170280 [Spinacia oleracea])

HSP 1 Score: 598.6 bits (1542), Expect = 6.000e-168
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 1


		  
Query: 1   MPLLPSPILAYRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTG 60
           MPLLPSPILAYRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTG
Sbjct: 1   MPLLPSPILAYRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTG 60

Query: 61  NSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYM 120
           NSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYM
Sbjct: 61  NSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYM 120

Query: 121 DSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAV 180
           DSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAV
Sbjct: 121 DSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAV 180

Query: 181 GIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIP 240
           GIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIP
Sbjct: 181 GIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIP 240

Query: 241 KALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           KALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG
Sbjct: 241 KALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 297

[Close]

BLAST of Spo08789.1 vs. NCBI nr
Match: gi|731368165|ref|XP_010695992.1| (PREDICTED: protein-L-isoaspartate O-methyltransferase-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 488.8 bits (1257), Expect = 6.700e-135
Identity = 258/314 (82.17%), Postives = 270/314 (85.99%), Query Frame = 1


		  
Query: 1   MPLLPSP---ILA------YRAPYFKHLTNFSS--------ILPHHHLPPLLLPRVPTAF 60
           M LLP P   ILA      YR P++KHLT  SS         L HH  PPLLLPRVPTA 
Sbjct: 1   MALLPKPSSSILAQGWRNCYRTPHYKHLTIISSPFFSSYTYTLLHH--PPLLLPRVPTAC 60

Query: 61  LRTFSLFNPCSPLSTTGNSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVM 120
           LRTFSLFN  S   TTGNSLL RMEK FSGSG G  KS+AMVE+L+RCGVFHSEKVAEVM
Sbjct: 61  LRTFSLFNHPSLSKTTGNSLLPRMEKSFSGSGDGKKKSEAMVERLQRCGVFHSEKVAEVM 120

Query: 121 ETIDRGLFVPAGTPAYMDSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTG 180
           ETIDRGLFVPAGTPAY+DSPMPIGHNATISAPHMHAMCL+LL D LQPGMHALDVGSGTG
Sbjct: 121 ETIDRGLFVPAGTPAYVDSPMPIGHNATISAPHMHAMCLELLKDKLQPGMHALDVGSGTG 180

Query: 181 YLTACFAVMVGSEGRAVGIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPE 240
           YLTACFA+MVG +GRAVGIEHIPELVTWSIENIKKS+AA LLEEGSLSVHVGDGR GYPE
Sbjct: 181 YLTACFAMMVGPKGRAVGIEHIPELVTWSIENIKKSEAAPLLEEGSLSVHVGDGRLGYPE 240

Query: 241 DAPYDAIHVGAAAPEIPKALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSV 298
            APYDAIHVGAAAPEIPKAL+EQLKPGGRLVIPVGTFFQDLQVIDK+QDGSLS RTETSV
Sbjct: 241 AAPYDAIHVGAAAPEIPKALVEQLKPGGRLVIPVGTFFQDLQVIDKEQDGSLSTRTETSV 300

[Close]

BLAST of Spo08789.1 vs. NCBI nr
Match: gi|731368167|ref|XP_010695993.1| (PREDICTED: protein-L-isoaspartate O-methyltransferase-like isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 424.1 bits (1089), Expect = 2.000e-115
Identity = 209/235 (88.94%), Postives = 221/235 (94.04%), Query Frame = 1


		  
Query: 63  LLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDS 122
           + S + K FSGSG G  KS+AMVE+L+RCGVFHSEKVAEVMETIDRGLFVPAGTPAY+DS
Sbjct: 16  IFSMLMKSFSGSGDGKKKSEAMVERLQRCGVFHSEKVAEVMETIDRGLFVPAGTPAYVDS 75

Query: 123 PMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGI 182
           PMPIGHNATISAPHMHAMCL+LL D LQPGMHALDVGSGTGYLTACFA+MVG +GRAVGI
Sbjct: 76  PMPIGHNATISAPHMHAMCLELLKDKLQPGMHALDVGSGTGYLTACFAMMVGPKGRAVGI 135

Query: 183 EHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKA 242
           EHIPELVTWSIENIKKS+AA LLEEGSLSVHVGDGR GYPE APYDAIHVGAAAPEIPKA
Sbjct: 136 EHIPELVTWSIENIKKSEAAPLLEEGSLSVHVGDGRLGYPEAAPYDAIHVGAAAPEIPKA 195

Query: 243 LIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           L+EQLKPGGRLVIPVGTFFQDLQVIDK+QDGSLS RTETSVRYVPLTSREEQLRG
Sbjct: 196 LVEQLKPGGRLVIPVGTFFQDLQVIDKEQDGSLSTRTETSVRYVPLTSREEQLRG 250

[Close]

BLAST of Spo08789.1 vs. NCBI nr
Match: gi|460381103|ref|XP_004236292.1| (PREDICTED: protein-L-isoaspartate O-methyltransferase 1 [Solanum lycopersicum])

HSP 1 Score: 385.2 bits (988), Expect = 1.000e-103
Identity = 198/287 (68.99%), Postives = 232/287 (80.84%), Query Frame = 1


		  
Query: 11  YRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTGNSLLSRMEKP 70
           YRAP  KH    + +L  HHL      + P   L + S     +P S TGNS  SRME+ 
Sbjct: 19  YRAP-LKH----ACLLYAHHLHSFHRQKSP---LFSSSFCRVLNPYSFTGNSRFSRMEQF 78

Query: 71  FSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNA 130
           +SGS G   ++K M EQLRRCGV  S+KV E+MET+DRGLFVP GTPAY+DSPM IG+NA
Sbjct: 79  WSGSAGN--RNKGMTEQLRRCGVIKSQKVIEIMETVDRGLFVPEGTPAYVDSPMSIGYNA 138

Query: 131 TISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVT 190
           TISAPHMHAMCL+LL D LQPGMHALDVGSGTGYLTACFA+MVG +GR VG+EHIPELV 
Sbjct: 139 TISAPHMHAMCLELLEDRLQPGMHALDVGSGTGYLTACFALMVGPQGRVVGVEHIPELVA 198

Query: 191 WSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPG 250
           WSI+N++KS AA LL+EGSLS+HVGDGR+G+PE APYDAIHVGAAA ++P+ LI+QLKPG
Sbjct: 199 WSIKNVEKSAAAPLLKEGSLSLHVGDGRKGWPEHAPYDAIHVGAAAADVPQDLIDQLKPG 258

Query: 251 GRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           GR+VIPVGTFFQDL+VIDKK DGS+SVR+ETSVRYVPLTSRE Q+ G
Sbjct: 259 GRMVIPVGTFFQDLKVIDKKLDGSISVRSETSVRYVPLTSREAQING 295

[Close]

BLAST of Spo08789.1 vs. NCBI nr
Match: gi|970013081|ref|XP_015067496.1| (PREDICTED: protein-L-isoaspartate O-methyltransferase 1-like [Solanum pennellii])

HSP 1 Score: 382.5 bits (981), Expect = 6.800e-103
Identity = 197/287 (68.64%), Postives = 234/287 (81.53%), Query Frame = 1


		  
Query: 11  YRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTGNSLLSRMEKP 70
           YRAP  KH    + +L  HHL      + P  F  +F  F   +P S TGNS  SRME+ 
Sbjct: 19  YRAP-LKH----TCLLYAHHLRSFHRQKSPL-FSSSFCRF--LNPYSFTGNSRFSRMEQF 78

Query: 71  FSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNA 130
           +SGS   + ++K M EQLRRCGV  S++V E+MET+DRGLFVP GTPAY+DSPM IG+NA
Sbjct: 79  WSGSA--VNRNKGMTEQLRRCGVIKSQEVIEIMETVDRGLFVPEGTPAYVDSPMSIGYNA 138

Query: 131 TISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVT 190
           TISAPHMHAMCL+LL D LQPGMHALDVGSGTGYLTACFA+MVG +GR VG+EHIPELV 
Sbjct: 139 TISAPHMHAMCLELLEDRLQPGMHALDVGSGTGYLTACFALMVGPQGRVVGVEHIPELVA 198

Query: 191 WSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPG 250
           WSI+N++KS AA LL+EGSLS+HVGDGR+G+PE APYDAIHVGAAA ++P+ LI+QLKPG
Sbjct: 199 WSIKNVEKSAAAPLLKEGSLSLHVGDGRKGWPEHAPYDAIHVGAAAADVPQDLIDQLKPG 258

Query: 251 GRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           GR+VIPVGTFFQDL+VIDKK DGS+SVR+ETSVRYVPLTSRE Q++G
Sbjct: 259 GRMVIPVGTFFQDLKVIDKKLDGSISVRSETSVRYVPLTSREAQIKG 295

[Close]

BLAST of Spo08789.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QK40_SPIOL (Protein-L-isoaspartate O-methyltransferase OS=Spinacia oleracea GN=SOVF_170280 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 4.200e-168
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 1


		  
Query: 1   MPLLPSPILAYRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTG 60
           MPLLPSPILAYRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTG
Sbjct: 1   MPLLPSPILAYRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTG 60

Query: 61  NSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYM 120
           NSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYM
Sbjct: 61  NSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYM 120

Query: 121 DSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAV 180
           DSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAV
Sbjct: 121 DSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAV 180

Query: 181 GIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIP 240
           GIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIP
Sbjct: 181 GIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIP 240

Query: 241 KALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           KALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG
Sbjct: 241 KALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 297

[Close]

BLAST of Spo08789.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E2C9_BETVU (Protein-L-isoaspartate O-methyltransferase OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g177100 PE=3 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 4.700e-135
Identity = 258/314 (82.17%), Postives = 270/314 (85.99%), Query Frame = 1


		  
Query: 1   MPLLPSP---ILA------YRAPYFKHLTNFSS--------ILPHHHLPPLLLPRVPTAF 60
           M LLP P   ILA      YR P++KHLT  SS         L HH  PPLLLPRVPTA 
Sbjct: 1   MALLPKPSSSILAQGWRNCYRTPHYKHLTIISSPFFSSYTYTLLHH--PPLLLPRVPTAC 60

Query: 61  LRTFSLFNPCSPLSTTGNSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVM 120
           LRTFSLFN  S   TTGNSLL RMEK FSGSG G  KS+AMVE+L+RCGVFHSEKVAEVM
Sbjct: 61  LRTFSLFNHPSLSKTTGNSLLPRMEKSFSGSGDGKKKSEAMVERLQRCGVFHSEKVAEVM 120

Query: 121 ETIDRGLFVPAGTPAYMDSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTG 180
           ETIDRGLFVPAGTPAY+DSPMPIGHNATISAPHMHAMCL+LL D LQPGMHALDVGSGTG
Sbjct: 121 ETIDRGLFVPAGTPAYVDSPMPIGHNATISAPHMHAMCLELLKDKLQPGMHALDVGSGTG 180

Query: 181 YLTACFAVMVGSEGRAVGIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPE 240
           YLTACFA+MVG +GRAVGIEHIPELVTWSIENIKKS+AA LLEEGSLSVHVGDGR GYPE
Sbjct: 181 YLTACFAMMVGPKGRAVGIEHIPELVTWSIENIKKSEAAPLLEEGSLSVHVGDGRLGYPE 240

Query: 241 DAPYDAIHVGAAAPEIPKALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSV 298
            APYDAIHVGAAAPEIPKAL+EQLKPGGRLVIPVGTFFQDLQVIDK+QDGSLS RTETSV
Sbjct: 241 AAPYDAIHVGAAAPEIPKALVEQLKPGGRLVIPVGTFFQDLQVIDKEQDGSLSTRTETSV 300

[Close]

BLAST of Spo08789.1 vs. UniProtKB/TrEMBL
Match: K4BK44_SOLLC (Protein-L-isoaspartate O-methyltransferase OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 7.300e-104
Identity = 198/287 (68.99%), Postives = 232/287 (80.84%), Query Frame = 1


		  
Query: 11  YRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTGNSLLSRMEKP 70
           YRAP  KH    + +L  HHL      + P   L + S     +P S TGNS  SRME+ 
Sbjct: 19  YRAP-LKH----ACLLYAHHLHSFHRQKSP---LFSSSFCRVLNPYSFTGNSRFSRMEQF 78

Query: 71  FSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNA 130
           +SGS G   ++K M EQLRRCGV  S+KV E+MET+DRGLFVP GTPAY+DSPM IG+NA
Sbjct: 79  WSGSAGN--RNKGMTEQLRRCGVIKSQKVIEIMETVDRGLFVPEGTPAYVDSPMSIGYNA 138

Query: 131 TISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVT 190
           TISAPHMHAMCL+LL D LQPGMHALDVGSGTGYLTACFA+MVG +GR VG+EHIPELV 
Sbjct: 139 TISAPHMHAMCLELLEDRLQPGMHALDVGSGTGYLTACFALMVGPQGRVVGVEHIPELVA 198

Query: 191 WSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPG 250
           WSI+N++KS AA LL+EGSLS+HVGDGR+G+PE APYDAIHVGAAA ++P+ LI+QLKPG
Sbjct: 199 WSIKNVEKSAAAPLLKEGSLSLHVGDGRKGWPEHAPYDAIHVGAAAADVPQDLIDQLKPG 258

Query: 251 GRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           GR+VIPVGTFFQDL+VIDKK DGS+SVR+ETSVRYVPLTSRE Q+ G
Sbjct: 259 GRMVIPVGTFFQDLKVIDKKLDGSISVRSETSVRYVPLTSREAQING 295

[Close]

BLAST of Spo08789.1 vs. UniProtKB/TrEMBL
Match: M1B870_SOLTU (Protein-L-isoaspartate O-methyltransferase OS=Solanum tuberosum GN=PGSC0003DMG400015226 PE=3 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 3.400e-101
Identity = 195/287 (67.94%), Postives = 231/287 (80.49%), Query Frame = 1


		  
Query: 11  YRAPYFKHLTNFSSILPHHHLPPLLLPRVPTAFLRTFSLFNPCSPLSTTGNSLLSRMEKP 70
           YRAP  KH    + +L  H L      + P   L + S     +P   TGNSL SRME+ 
Sbjct: 19  YRAP-LKH----TCLLYAHQLHSSHRQKSP---LFSSSFCRVLNPSFFTGNSLFSRMEQF 78

Query: 71  FSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNA 130
           +SGS   + ++K M EQLRR GV  S+KV E+MET+DRGLFVP GTPAY+DSPM IG+NA
Sbjct: 79  WSGSA--VNRNKGMTEQLRRYGVIKSQKVTEIMETVDRGLFVPEGTPAYIDSPMSIGYNA 138

Query: 131 TISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVT 190
           TISAPHMHAMCL+LL D LQPGMHALDVGSGTGYLTACFA+MVG +GR VG+EHIPELV 
Sbjct: 139 TISAPHMHAMCLELLEDRLQPGMHALDVGSGTGYLTACFALMVGPQGRVVGVEHIPELVA 198

Query: 191 WSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPG 250
           WSI+N++KS AA LL+EGSLS+HVGDGR+G+PE APYDAIHVGAAA ++P+ LI+QLKPG
Sbjct: 199 WSIKNVEKSAAAPLLKEGSLSLHVGDGRKGWPEHAPYDAIHVGAAAADVPQDLIDQLKPG 258

Query: 251 GRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           GR+VIPVGTFFQDL+VIDKK DGS+SVR+ETSVRYVPLTSRE Q++G
Sbjct: 259 GRMVIPVGTFFQDLKVIDKKMDGSISVRSETSVRYVPLTSREAQIKG 295

[Close]

BLAST of Spo08789.1 vs. UniProtKB/TrEMBL
Match: A0A022PPV1_ERYGU (Protein-L-isoaspartate O-methyltransferase OS=Erythranthe guttata GN=MIMGU_mgv1a010627mg PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 9.800e-101
Identity = 200/292 (68.49%), Postives = 229/292 (78.42%), Query Frame = 1


		  
Query: 11  YRAPYFKHLTNFSSI-LPHHHLPPLLLPRVPTAFLRTFSLFNPC----SPLSTTGNSLLS 70
           YRAP      N +   L HH L  +  P +  A   TFS  + C     P  +TGNSL S
Sbjct: 18  YRAPLRLSFLNLTPPPLNHHRLCIIPPPHLAAAAAATFS--SRCCRLPDPHPSTGNSLFS 77

Query: 71  RMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMP 130
            ME+ +SGS   ++K+K MVE L+  GV  S KVAEVMETIDR LFVP GT  Y+DSPM 
Sbjct: 78  SMERFWSGSS--ISKNKEMVEHLKSYGVMRSSKVAEVMETIDRALFVPEGTQPYVDSPMQ 137

Query: 131 IGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHI 190
           IG+NATISAPHMHAMCL+LL ++L+PGMHALD+GSGTGYLTACFAVMVGS+GRAVG+EHI
Sbjct: 138 IGYNATISAPHMHAMCLELLENHLKPGMHALDIGSGTGYLTACFAVMVGSQGRAVGVEHI 197

Query: 191 PELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIE 250
           PELV  SI+NIKKS+AA L E+GS SVHVGDGRQG+PE APYDAIHVGAAAPEIP ALIE
Sbjct: 198 PELVETSIKNIKKSEAAPLFEDGSFSVHVGDGRQGWPEFAPYDAIHVGAAAPEIPPALIE 257

Query: 251 QLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           QLKPGGRLVIPVG  FQDLQV+DK  DG+LSVR+ETSVRYVPLTSR+ Q+RG
Sbjct: 258 QLKPGGRLVIPVGNVFQDLQVVDKNTDGTLSVRSETSVRYVPLTSRDSQIRG 305

[Close]

BLAST of Spo08789.1 vs. ExPASy Swiss-Prot
Match: PIMT_WHEAT (Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum GN=PCM PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 4.300e-89
Identity = 161/221 (72.85%), Postives = 187/221 (84.62%), Query Frame = 1


		  
Query: 76  GGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNATISAP 135
           G + K+ A+VE L++ GV  ++KVAEVMETIDR LFVP G   Y DSPMPIG+NATISAP
Sbjct: 8   GSLEKNNALVEYLKQYGVVRTDKVAEVMETIDRALFVPEGFTPYTDSPMPIGYNATISAP 67

Query: 136 HMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVTWSIEN 195
           HMHA CL+LL D LQPGMHALDVGSG+GYLTACFA+MVG EGRAVGIEHIPELV  S EN
Sbjct: 68  HMHATCLELLKDYLQPGMHALDVGSGSGYLTACFAMMVGPEGRAVGIEHIPELVVASTEN 127

Query: 196 IKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPGGRLVI 255
           +++S AA+L+++GSLS HV DGR G+P+ APYDAIHVGAAAPEIP+ L+EQLKPGGR+VI
Sbjct: 128 VERSAAAALMKDGSLSFHVSDGRLGWPDAAPYDAIHVGAAAPEIPRPLLEQLKPGGRMVI 187

Query: 256 PVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLR 297
           PVGT+ QDLQVIDK  DGS SVR + SVRYVPLTSR  QL+
Sbjct: 188 PVGTYSQDLQVIDKSADGSTSVRNDASVRYVPLTSRSAQLQ 228

[Close]

BLAST of Spo08789.1 vs. ExPASy Swiss-Prot
Match: PIMT2_ARATH (Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana GN=PIMT2 PE=1 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 5.600e-89
Identity = 163/242 (67.36%), Postives = 199/242 (82.23%), Query Frame = 1


		  
Query: 56  LSTTGNSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAG 115
           LS    +L   ME+  SG+G   +  + MVE L+R GV  S++VA+VME +DRGLFVP G
Sbjct: 69  LSRFSGALFFHMEQFQSGTGS--SGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVG 128

Query: 116 TPAYMDSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGS 175
           + AY+D+P+PIG+NATISAPHMHA CL LL D L PGM ALDVGSGTGYLT CFA+MVG+
Sbjct: 129 SSAYVDTPVPIGYNATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGA 188

Query: 176 EGRAVGIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAA 235
           EGR VG++HIPELV  SI+NI+KS AAS L++GSLS+HVGDGR+G+ E APYDAIHVGAA
Sbjct: 189 EGRVVGVDHIPELVDMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAA 248

Query: 236 APEIPKALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQL 295
           A EIP+ L++QLKPGGR+VIP+GT+FQ+L+VIDK +DGS+ V TETSVRYVPLTSR EQL
Sbjct: 249 ASEIPQPLLDQLKPGGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 308

Query: 296 RG 298
            G
Sbjct: 309 GG 308

[Close]

BLAST of Spo08789.1 vs. ExPASy Swiss-Prot
Match: PIMT1_ARATH (Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana GN=PIMT1 PE=1 SV=3)

HSP 1 Score: 323.6 bits (828), Expect = 2.300e-87
Identity = 158/224 (70.54%), Postives = 188/224 (83.93%), Query Frame = 1


		  
Query: 74  SGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNATIS 133
           S   + K+KAMVE L+  G+  S++VA+ ME +DRG+FV   + AY+DSPM IG+N TIS
Sbjct: 6   SPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYNVTIS 65

Query: 134 APHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVTWSI 193
           APHMHAMCL LL  +L+PGM  LDVGSGTGYLTACFAVMVG+EGRA+G+EHIPELV  S+
Sbjct: 66  APHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELVASSV 125

Query: 194 ENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPGGRL 253
           +NI+ S A+  L+EGSL+VHVGDGRQG+ E APYDAIHVGAAAPEIP+ALI+QLKPGGRL
Sbjct: 126 KNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKPGGRL 185

Query: 254 VIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           VIPVG  FQDLQV+DK  DGS+S++ ETSVRYVPLTSRE QLRG
Sbjct: 186 VIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLRG 229

[Close]

BLAST of Spo08789.1 vs. ExPASy Swiss-Prot
Match: PIMT_MOUSE (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Mus musculus GN=Pcmt1 PE=1 SV=3)

HSP 1 Score: 223.0 bits (567), Expect = 4.300e-57
Identity = 121/220 (55.00%), Postives = 150/220 (68.18%), Query Frame = 1


		  
Query: 77  GMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNATISAPH 136
           G A    ++  LR+ G+  ++KV EVM   DR  +  A +  YMDSP  IG  ATISAPH
Sbjct: 6   GGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHY--AKSNPYMDSPQSIGFQATISAPH 65

Query: 137 MHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVTWSIENI 196
           MHA  L+LL D L  G  ALDVGSG+G LTACFA MVG+ G+ +GI+HI ELV  SI N+
Sbjct: 66  MHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGNSGKVIGIDHIKELVDDSITNV 125

Query: 197 KKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPGGRLVIP 256
           KK     LL  G + + VGDGR GY E+APYDAIHVGAAAP +P+ALI+QLKPGGRL++P
Sbjct: 126 KKDD-PMLLSSGRVRLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILP 185

Query: 257 VGTF--FQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQ 295
           VG     Q L+  DK QDGS+ ++    V YVPLT +E+Q
Sbjct: 186 VGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 222

[Close]

BLAST of Spo08789.1 vs. ExPASy Swiss-Prot
Match: PIMT_CHICK (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus GN=PCMT1 PE=2 SV=3)

HSP 1 Score: 222.2 bits (565), Expect = 7.300e-57
Identity = 121/220 (55.00%), Postives = 151/220 (68.64%), Query Frame = 1


		  
Query: 77  GMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNATISAPH 136
           G A    ++  LR+ G+  S+KV EVM   DR  +  A    YMDSP  IG  ATISAPH
Sbjct: 6   GGASHSELIHNLRKNGIIKSDKVFEVMLATDRCHY--AKYNPYMDSPQSIGFQATISAPH 65

Query: 137 MHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVTWSIENI 196
           MHA  L+LL+D L  G  ALDVGSG+G LTACF+ MVG +G+ VGI+HI ELV  SI N+
Sbjct: 66  MHAYALELLSDQLHEGAKALDVGSGSGILTACFSRMVGPKGQVVGIDHIKELVDDSINNV 125

Query: 197 KKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPGGRLVIP 256
           KK    +LL  G + + VGDGR GY E+APYDAIHVGAAAP +P+ALI+QLKPGGRL++P
Sbjct: 126 KKDD-PTLLSSGRVKLIVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILP 185

Query: 257 VGTF--FQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQ 295
           VG     Q L+  DK +DGS+ ++    V YVPLT +E+Q
Sbjct: 186 VGPAGGNQMLEQYDKLEDGSVKMKPLMGVIYVPLTDKEKQ 222

[Close]

BLAST of Spo08789.1 vs. TAIR (Arabidopsis)
Match: AT5G50240.1 (protein-l-isoaspartate methyltransferase 2)

HSP 1 Score: 328.9 bits (842), Expect = 3.100e-90
Identity = 163/242 (67.36%), Postives = 199/242 (82.23%), Query Frame = 1


		  
Query: 56  LSTTGNSLLSRMEKPFSGSGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAG 115
           LS    +L   ME+  SG+G   +  + MVE L+R GV  S++VA+VME +DRGLFVP G
Sbjct: 69  LSRFSGALFFHMEQFQSGTGS--SGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVG 128

Query: 116 TPAYMDSPMPIGHNATISAPHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGS 175
           + AY+D+P+PIG+NATISAPHMHA CL LL D L PGM ALDVGSGTGYLT CFA+MVG+
Sbjct: 129 SSAYVDTPVPIGYNATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGA 188

Query: 176 EGRAVGIEHIPELVTWSIENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAA 235
           EGR VG++HIPELV  SI+NI+KS AAS L++GSLS+HVGDGR+G+ E APYDAIHVGAA
Sbjct: 189 EGRVVGVDHIPELVDMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAA 248

Query: 236 APEIPKALIEQLKPGGRLVIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQL 295
           A EIP+ L++QLKPGGR+VIP+GT+FQ+L+VIDK +DGS+ V TETSVRYVPLTSR EQL
Sbjct: 249 ASEIPQPLLDQLKPGGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 308

Query: 296 RG 298
            G
Sbjct: 309 GG 308

[Close]

BLAST of Spo08789.1 vs. TAIR (Arabidopsis)
Match: AT3G48330.1 (protein-l-isoaspartate methyltransferase 1)

HSP 1 Score: 323.6 bits (828), Expect = 1.300e-88
Identity = 158/224 (70.54%), Postives = 188/224 (83.93%), Query Frame = 1


		  
Query: 74  SGGGMAKSKAMVEQLRRCGVFHSEKVAEVMETIDRGLFVPAGTPAYMDSPMPIGHNATIS 133
           S   + K+KAMVE L+  G+  S++VA+ ME +DRG+FV   + AY+DSPM IG+N TIS
Sbjct: 6   SPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYNVTIS 65

Query: 134 APHMHAMCLDLLNDNLQPGMHALDVGSGTGYLTACFAVMVGSEGRAVGIEHIPELVTWSI 193
           APHMHAMCL LL  +L+PGM  LDVGSGTGYLTACFAVMVG+EGRA+G+EHIPELV  S+
Sbjct: 66  APHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELVASSV 125

Query: 194 ENIKKSKAASLLEEGSLSVHVGDGRQGYPEDAPYDAIHVGAAAPEIPKALIEQLKPGGRL 253
           +NI+ S A+  L+EGSL+VHVGDGRQG+ E APYDAIHVGAAAPEIP+ALI+QLKPGGRL
Sbjct: 126 KNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKPGGRL 185

Query: 254 VIPVGTFFQDLQVIDKKQDGSLSVRTETSVRYVPLTSREEQLRG 298
           VIPVG  FQDLQV+DK  DGS+S++ ETSVRYVPLTSRE QLRG
Sbjct: 186 VIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLRG 229

The following BLAST results are available for this feature:

BLAST of Spo08789.1 vs. NCBI nr
BLAST of Spo08789.1 vs. UniProtKB/TrEMBL
BLAST of Spo08789.1 vs. ExPASy Swiss-Prot
BLAST of Spo08789.1 vs. TAIR (Arabidopsis)

BLAST of Spo08789.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902169091\|gb\|KNA07602.1\|	6.0e-168	100.	hypothetical protein SOVF_1702...	[more]
gi\|731368165\|ref\|XP_010695992.1\|	6.7e-135	82.1	PREDICTED: protein-L-isoaspart...	[more]
gi\|731368167\|ref\|XP_010695993.1\|	2.0e-115	88.9	PREDICTED: protein-L-isoaspart...	[more]
gi\|460381103\|ref\|XP_004236292.1\|	1.0e-103	68.9	PREDICTED: protein-L-isoaspart...	[more]
gi\|970013081\|ref\|XP_015067496.1\|	6.8e-103	68.6	PREDICTED: protein-L-isoaspart...	[more]

BLAST of Spo08789.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QK40_SPIOL	4.2e-168	100.	Protein-L-isoaspartate O-methy...	[more]
A0A0J8E2C9_BETVU	4.7e-135	82.1	Protein-L-isoaspartate O-methy...	[more]
K4BK44_SOLLC	7.3e-104	68.9	Protein-L-isoaspartate O-methy...	[more]
M1B870_SOLTU	3.4e-101	67.9	Protein-L-isoaspartate O-methy...	[more]
A0A022PPV1_ERYGU	9.8e-101	68.4	Protein-L-isoaspartate O-methy...	[more]

BLAST of Spo08789.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
PIMT_WHEAT	4.3e-89	72.8	Protein-L-isoaspartate O-methy...	[more]
PIMT2_ARATH	5.6e-89	67.3	Protein-L-isoaspartate O-methy...	[more]
PIMT1_ARATH	2.3e-87	70.5	Protein-L-isoaspartate O-methy...	[more]
PIMT_MOUSE	4.3e-57	55.0	Protein-L-isoaspartate(D-aspar...	[more]
PIMT_CHICK	7.3e-57	55.0	Protein-L-isoaspartate(D-aspar...	[more]

BLAST of Spo08789.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G50240.1	3.1e-90	67.3	protein-l-isoaspartate methylt...	[more]
AT3G48330.1	1.3e-88	70.5	protein-l-isoaspartate methylt...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	PANTHER	PTHR11579	PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE	coord: 74..296 score: 1.8E
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	TIGRFAMs	TIGR00080	TIGR00080	coord: 80..294 score: 1.9
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	PROSITE	PS01279	PCMT	coord: 215..230 scor
None	No IPR available	PANTHER	PTHR11579:SF8	SUBFAMILY NOT NAMED	coord: 74..296 score: 1.8E
None	No IPR available	PFAM	PF01135	PCMT	coord: 80..292 score: 3.3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process
biological_process	GO:0006479	protein methylation
biological_process	GO:0046500	S-adenosylmethionine metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004719	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity