Spo08729.1 (mRNA) | SpinachBase

Overview


Name	Spo08729.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	chlororespiratory reduction 7
Location	SpoScf_02373 : 46763 .. 47350 (+)
Sequence length	537

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACCAATTGCCCGAATATTGAATACCCAGCAACATGCCATCTACATTGGTCATCATCTCATTCCATTTCGTAAGCCTTGGCCAAAACTTGTTTTCTCCCATTCACTCTAACATTATTAACTCTCTGGTTTTTCCTAACAGATAAACCATGCTCGAGGATATACCGCCACTTCCCGGAAATCCCTCCACAGCAAGTTTGTGTTGGACAGCTGCTTTCGTGCAGGAGTGATGTTAAGGCAAGTCCGAATTGAACATTTTATTATCATTGAATTTTTGTTAATGTTGTATGGAAATTTGGGTGTTTCACAGAGTTATGCAGGTAGGAGGAGAAGGTCGAATTACATGGAGACAGATACTTATGTTTACTTGGAACCAGGGAGAAGTGAAGAGTTTGTTTCTGAAGAAGAATTGAAGGTTAAACTTAAAGGATGGCTAGAAAATTGGCCAAACAAGACGTTACCACCCGACCTTGCTAGATTCCAAACATTGGATGATACAGTCACTTTTCTGGTCAAGTCTGTATGTGAGCTTGAAATTGATGGAGATGTTGGTTCTATCCAATGGTATCAGGTCCGTCTTGACTAA

mRNA sequence

ATGACACCAATTGCCCGAATATTGAATACCCAGCAACATGCCATCTACATTGGTCATCATCTCATTCCATTTCGTAAGCCTTGGCCAAAACTTATAAACCATGCTCGAGGATATACCGCCACTTCCCGGAAATCCCTCCACAGCAAGTTTGTGTTGGACAGCTGCTTTCGTGCAGGAGTGATGTTAAGGCAAGTCCGAATTGAACATTTTATTATCATTGAATTTTTGTTAATGTTGTATGGAAATTTGGGTGTTTCACAGAGTTATGCAGGTAGGAGGAGAAGGTCGAATTACATGGAGACAGATACTTATGTTTACTTGGAACCAGGGAGAAGTGAAGAGTTTGTTTCTGAAGAAGAATTGAAGGTTAAACTTAAAGGATGGCTAGAAAATTGGCCAAACAAGACGTTACCACCCGACCTTGCTAGATTCCAAACATTGGATGATACAGTCACTTTTCTGGTCAAGTCTGTATGTGAGCTTGAAATTGATGGAGATGTTGGTTCTATCCAATGGTATCAGGTCCGTCTTGACTAA

Coding sequence (CDS)

ATGACACCAATTGCCCGAATATTGAATACCCAGCAACATGCCATCTACATTGGTCATCATCTCATTCCATTTCGTAAGCCTTGGCCAAAACTTATAAACCATGCTCGAGGATATACCGCCACTTCCCGGAAATCCCTCCACAGCAAGTTTGTGTTGGACAGCTGCTTTCGTGCAGGAGTGATGTTAAGGCAAGTCCGAATTGAACATTTTATTATCATTGAATTTTTGTTAATGTTGTATGGAAATTTGGGTGTTTCACAGAGTTATGCAGGTAGGAGGAGAAGGTCGAATTACATGGAGACAGATACTTATGTTTACTTGGAACCAGGGAGAAGTGAAGAGTTTGTTTCTGAAGAAGAATTGAAGGTTAAACTTAAAGGATGGCTAGAAAATTGGCCAAACAAGACGTTACCACCCGACCTTGCTAGATTCCAAACATTGGATGATACAGTCACTTTTCTGGTCAAGTCTGTATGTGAGCTTGAAATTGATGGAGATGTTGGTTCTATCCAATGGTATCAGGTCCGTCTTGACTAA

Protein sequence

MTPIARILNTQQHAIYIGHHLIPFRKPWPKLINHARGYTATSRKSLHSKFVLDSCFRAGVMLRQVRIEHFIIIEFLLMLYGNLGVSQSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo08729	Spo08729	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo08729.1	Spo08729.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08729.1.exon.1	Spo08729.1.exon.1	exon
Spo08729.1.exon.2	Spo08729.1.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08729.1.CDS.1	Spo08729.1.CDS.1	CDS
Spo08729.1.CDS.2	Spo08729.1.CDS.2	CDS

Homology

[Close]

BLAST of Spo08729.1 vs. NCBI nr
Match: gi|902147349|gb|KNA05149.1| (hypothetical protein SOVF_193040 isoform A [Spinacia oleracea])

HSP 1 Score: 221.9 bits (564), Expect = 9.200e-55
Identity = 121/178 (67.98%), Postives = 126/178 (70.79%), Query Frame = 1


		  
Query: 1   MTPIARILNTQQHAIYIGHHLIPFRKPWPKLINHARGYTATSRKSLHSKFVLDSCFRAGV 60
           MTPIARILNTQQHAIYIGHHLIPF             +   SR   H   +       G 
Sbjct: 1   MTPIARILNTQQHAIYIGHHLIPF-------------HKPCSRIYRHFPEIPPQQVCVGQ 60

Query: 61  MLRQVRIEHFIIIEFLLMLYGNLGVSQSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEE 120
           +L   R +                  +SYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEE
Sbjct: 61  LL-SCRSD-----------------VKSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEE 120

Query: 121 LKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           LKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD
Sbjct: 121 LKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 147

[Close]

BLAST of Spo08729.1 vs. NCBI nr
Match: gi|902147350|gb|KNA05150.1| (hypothetical protein SOVF_193040 isoform B [Spinacia oleracea])

HSP 1 Score: 193.0 bits (489), Expect = 4.600e-46
Identity = 91/92 (98.91%), Postives = 92/92 (100.00%), Query Frame = 1


		  
Query: 87  QSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQT 146
           +SYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQT
Sbjct: 49  KSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQT 108

Query: 147 LDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           LDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD
Sbjct: 109 LDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 140

[Close]

BLAST of Spo08729.1 vs. NCBI nr
Match: gi|731319261|ref|XP_010670166.1| (PREDICTED: uncharacterized protein LOC104887258 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 185.7 bits (470), Expect = 7.300e-44
Identity = 99/178 (55.62%), Postives = 116/178 (65.17%), Query Frame = 1


		  
Query: 1   MTPIARILNTQQHAIYIGHHLIPFRKPWPKLINHARGYTATSRKSLHSKFVLDSCFRAGV 60
           M P  RI N+QQ +I+  HH IP            RG                    A +
Sbjct: 1   MIPATRIFNSQQQSIFNAHHPIPISS------QRLRGV-------------------AEI 60

Query: 61  MLRQVRIEHFIIIEFLLMLYGNLGVSQSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEE 120
             +QV + H     +  +L  +    + YAGRRRRSNYM+TDTYVYLEPG+SEEFVSEEE
Sbjct: 61  PQQQVCLVHLDTSAYAKLLRISNSEVKIYAGRRRRSNYMDTDTYVYLEPGKSEEFVSEEE 120

Query: 121 LKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           LK+KLK WLENWPNK LPPDLARF+TLDD V+FLVKSVCELEIDGDVGSIQWY+VRL+
Sbjct: 121 LKMKLKVWLENWPNKVLPPDLARFETLDDAVSFLVKSVCELEIDGDVGSIQWYEVRLE 153

[Close]

BLAST of Spo08729.1 vs. NCBI nr
Match: gi|971579280|ref|XP_015158923.1| (PREDICTED: protein CHLORORESPIRATORY REDUCTION 7, chloroplastic isoform X1 [Solanum tuberosum])

HSP 1 Score: 151.0 bits (380), Expect = 2.000e-33
Identity = 71/95 (74.74%), Postives = 83/95 (87.37%), Query Frame = 1


		  
Query: 83  LGVSQSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLA 142
           + +S++ A RRRRSN +ETDTYV +EPG+SEEFV+EEEL+ KLK WLENWP KTLPPDLA
Sbjct: 61  VSLSKTLATRRRRSN-IETDTYVLMEPGKSEEFVTEEELRDKLKSWLENWPGKTLPPDLA 120

Query: 143 RFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRL 178
           RF+  DD V +LVKSVCELEIDGDVGS+QWYQVRL
Sbjct: 121 RFEDTDDAVEYLVKSVCELEIDGDVGSLQWYQVRL 154

[Close]

BLAST of Spo08729.1 vs. NCBI nr
Match: gi|296082204|emb|CBI21209.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 150.2 bits (378), Expect = 3.400e-33
Identity = 67/92 (72.83%), Postives = 82/92 (89.13%), Query Frame = 1


		  
Query: 87  QSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQT 146
           +SYA RRRR+ Y +T+TYV +EPG+ EEFVS+EELK +LKGWLENWP K LPPDLA+FQT
Sbjct: 61  KSYAMRRRRA-YSQTETYVLMEPGKDEEFVSQEELKARLKGWLENWPGKALPPDLAKFQT 120

Query: 147 LDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           +DD V +LVK+VCELEIDGDVGSIQWY++RL+
Sbjct: 121 IDDAVMYLVKAVCELEIDGDVGSIQWYEIRLE 151

[Close]

BLAST of Spo08729.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QD04_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_193040 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 6.400e-55
Identity = 121/178 (67.98%), Postives = 126/178 (70.79%), Query Frame = 1


		  
Query: 1   MTPIARILNTQQHAIYIGHHLIPFRKPWPKLINHARGYTATSRKSLHSKFVLDSCFRAGV 60
           MTPIARILNTQQHAIYIGHHLIPF             +   SR   H   +       G 
Sbjct: 1   MTPIARILNTQQHAIYIGHHLIPF-------------HKPCSRIYRHFPEIPPQQVCVGQ 60

Query: 61  MLRQVRIEHFIIIEFLLMLYGNLGVSQSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEE 120
           +L   R +                  +SYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEE
Sbjct: 61  LL-SCRSD-----------------VKSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEE 120

Query: 121 LKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           LKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD
Sbjct: 121 LKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 147

[Close]

BLAST of Spo08729.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QD32_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_193040 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 3.200e-46
Identity = 91/92 (98.91%), Postives = 92/92 (100.00%), Query Frame = 1


		  
Query: 87  QSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQT 146
           +SYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQT
Sbjct: 49  KSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQT 108

Query: 147 LDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           LDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD
Sbjct: 109 LDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 140

[Close]

BLAST of Spo08729.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CZB8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g039630 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 5.100e-44
Identity = 99/178 (55.62%), Postives = 116/178 (65.17%), Query Frame = 1


		  
Query: 1   MTPIARILNTQQHAIYIGHHLIPFRKPWPKLINHARGYTATSRKSLHSKFVLDSCFRAGV 60
           M P  RI N+QQ +I+  HH IP            RG                    A +
Sbjct: 1   MIPATRIFNSQQQSIFNAHHPIPISS------QRLRGV-------------------AEI 60

Query: 61  MLRQVRIEHFIIIEFLLMLYGNLGVSQSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEE 120
             +QV + H     +  +L  +    + YAGRRRRSNYM+TDTYVYLEPG+SEEFVSEEE
Sbjct: 61  PQQQVCLVHLDTSAYAKLLRISNSEVKIYAGRRRRSNYMDTDTYVYLEPGKSEEFVSEEE 120

Query: 121 LKVKLKGWLENWPNKTLPPDLARFQTLDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           LK+KLK WLENWPNK LPPDLARF+TLDD V+FLVKSVCELEIDGDVGSIQWY+VRL+
Sbjct: 121 LKMKLKVWLENWPNKVLPPDLARFETLDDAVSFLVKSVCELEIDGDVGSIQWYEVRLE 153

[Close]

BLAST of Spo08729.1 vs. UniProtKB/TrEMBL
Match: F6H488_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0068g00280 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.400e-33
Identity = 67/92 (72.83%), Postives = 82/92 (89.13%), Query Frame = 1


		  
Query: 87  QSYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQT 146
           +SYA RRRR+ Y +T+TYV +EPG+ EEFVS+EELK +LKGWLENWP K LPPDLA+FQT
Sbjct: 68  KSYAMRRRRA-YSQTETYVLMEPGKDEEFVSQEELKARLKGWLENWPGKALPPDLAKFQT 127

Query: 147 LDDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           +DD V +LVK+VCELEIDGDVGSIQWY++RL+
Sbjct: 128 IDDAVMYLVKAVCELEIDGDVGSIQWYEIRLE 158

[Close]

BLAST of Spo08729.1 vs. UniProtKB/TrEMBL
Match: A0A061EN03_THECC (Chlororespiratory reduction 7 isoform 1 OS=Theobroma cacao GN=TCM_047041 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 5.300e-33
Identity = 68/89 (76.40%), Postives = 78/89 (87.64%), Query Frame = 1


		  
Query: 90  AGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQTLDD 149
           A RRRR+ Y  T+TYV LEPG+ E+FVS EELK KLKGWLENWP KTLPPDLARF T+DD
Sbjct: 61  ATRRRRAAYSRTETYVLLEPGQEEKFVSVEELKAKLKGWLENWPGKTLPPDLARFVTIDD 120

Query: 150 TVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
            V++LV+SVCELEIDGDVGSIQWY+VRL+
Sbjct: 121 AVSYLVRSVCELEIDGDVGSIQWYEVRLE 149

[Close]

BLAST of Spo08729.1 vs. ExPASy Swiss-Prot
Match: CRR7_ARATH (Protein CHLORORESPIRATORY REDUCTION 7, chloroplastic OS=Arabidopsis thaliana GN=CRR7 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.700e-30
Identity = 60/91 (65.93%), Postives = 73/91 (80.22%), Query Frame = 1


		  
Query: 88  SYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQTL 147
           S    RRR  +  +DTYV LE G+ E+FV+E+ELK KL+GWLENWP  +LPPDLARF  L
Sbjct: 66  SVCATRRRRVHSNSDTYVLLEAGQDEQFVTEDELKAKLRGWLENWPVNSLPPDLARFDDL 125

Query: 148 DDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           D+ V FLVK+VCELEIDG+VGS+QWYQVRL+
Sbjct: 126 DEAVDFLVKAVCELEIDGEVGSVQWYQVRLE 156

[Close]

BLAST of Spo08729.1 vs. TAIR (Arabidopsis)
Match: AT5G39210.1 (chlororespiratory reduction 7)

HSP 1 Score: 133.3 bits (334), Expect = 1.500e-31
Identity = 60/91 (65.93%), Postives = 73/91 (80.22%), Query Frame = 1


		  
Query: 88  SYAGRRRRSNYMETDTYVYLEPGRSEEFVSEEELKVKLKGWLENWPNKTLPPDLARFQTL 147
           S    RRR  +  +DTYV LE G+ E+FV+E+ELK KL+GWLENWP  +LPPDLARF  L
Sbjct: 66  SVCATRRRRVHSNSDTYVLLEAGQDEQFVTEDELKAKLRGWLENWPVNSLPPDLARFDDL 125

Query: 148 DDTVTFLVKSVCELEIDGDVGSIQWYQVRLD 179
           D+ V FLVK+VCELEIDG+VGS+QWYQVRL+
Sbjct: 126 DEAVDFLVKAVCELEIDGEVGSVQWYQVRLE 156

The following BLAST results are available for this feature:

BLAST of Spo08729.1 vs. NCBI nr
BLAST of Spo08729.1 vs. UniProtKB/TrEMBL
BLAST of Spo08729.1 vs. ExPASy Swiss-Prot
BLAST of Spo08729.1 vs. TAIR (Arabidopsis)

BLAST of Spo08729.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902147349\|gb\|KNA05149.1\|	9.2e-55	67.9	hypothetical protein SOVF_1930...	[more]
gi\|902147350\|gb\|KNA05150.1\|	4.6e-46	98.9	hypothetical protein SOVF_1930...	[more]
gi\|731319261\|ref\|XP_010670166.1\|	7.3e-44	55.6	PREDICTED: uncharacterized pro...	[more]
gi\|971579280\|ref\|XP_015158923.1\|	2.0e-33	74.7	PREDICTED: protein CHLORORESPI...	[more]
gi\|296082204\|emb\|CBI21209.3\|	3.4e-33	72.8	unnamed protein product [Vitis...	[more]

BLAST of Spo08729.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QD04_SPIOL	6.4e-55	67.9	Uncharacterized protein OS=Spi...	[more]
A0A0K9QD32_SPIOL	3.2e-46	98.9	Uncharacterized protein OS=Spi...	[more]
A0A0J8CZB8_BETVU	5.1e-44	55.6	Uncharacterized protein OS=Bet...	[more]
F6H488_VITVI	2.4e-33	72.8	Putative uncharacterized prote...	[more]
A0A061EN03_THECC	5.3e-33	76.4	Chlororespiratory reduction 7 ...	[more]

BLAST of Spo08729.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1


Match Name	E-value	Identity	Description
CRR7_ARATH	2.7e-30	65.9	Protein CHLORORESPIRATORY REDU...	[more]

BLAST of Spo08729.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G39210.1	1.5e-31	65.9	chlororespiratory reduction 7	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021954	Protein CHLORORESPIRATORY REDUCTION 7	PFAM	PF12095	DUF3571	coord: 98..178 score: 1.6
None	No IPR available	unknown	Coil	Coil	coord: 170..178 scor
None	No IPR available	PANTHER	PTHR36803	FAMILY NOT NAMED	coord: 60..178 score: 3.4

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000023	maltose metabolic process
biological_process	GO:0016556	mRNA modification
biological_process	GO:0010275	NAD(P)H dehydrogenase complex assembly
biological_process	GO:0010207	photosystem II assembly
biological_process	GO:0019252	starch biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0010598	NAD(P)H dehydrogenase complex (plastoquinone)