Spo07733.1 (mRNA)

Overview
NameSpo07733.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Locationchr6 : 28590110 .. 28591614 (-)
Sequence length595
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGGAATTTCTCCTATTTGCCTGCCGCGACTCGAAAAATCAATAATGAACAATTACATGCTGCAACCAGTTTCATTCATTCACAGAGTGACAAAACCCAGAAAAAAAAAATGCTCTGAAATTTAAACCTGCAGCAAAAAATGGGGAAGAACAGCAAGAAGCAGAGGCATCAACAGAAACCTTTAGCTCGTCAAAGAAACTCTAATTTTCCTCCAGAAGATGAACATTACCCTCCTCTTTCTCTCCCCAATAGCGAAGAAGAAACCCCAGAAGAAGAAGAACCTGAACCAGAAATTCACCAAACCCCTTCTTCCGATGATTCAATTCCTTCAAAGTTTTCTCTCTATCAACAATCTGTTCAGGTACTTTCAATTTAACAAATATCTTTTAGTTGAATACTAGAATTGTTAATTATGGTTAATTTGTTATGATTTTAATTAGGTATTTTGTGTTATTGTTGTTGTTTTCAAATGTAGTCACCAAAAGGGGATATAAGCTATTTGCAGAAGTTTTTCTTGATGTATGTTGGTGGGAGGGCTCCTCTTCATCTACAGGAGGATTTTTGTGGCACTGCTCTTATTAGGTAAAATTTTTACTTCTTCATCATGTATGATTTTTATGTCTAATGGAGGATTTGTTGTTGTCGTTGTCATTGTCATCTTCATCGTCATCACTGTCTCTATTTGTGGAACTAGTAATGCTCATACTTTAAAGGAGAGAAAGAACAAATATGATGGGCCGATTTTGTGAGGTTATATGAGTACATTGAGGTAATCCATATAATGCACCCTTATGACTGGATATTCAACTATTCAAATCACATTGTCCGAACTCCACATAATTAAGCACTTGGCATGATCCGACTTAAAACCTCCCTTATTATAAGGTACTAATAAGGTACTTGGGAATGGGAATGGCAATGACAATGACAATGCTGCTCAACCTTTTTTGGACATTGAGTATATATATAGTGTGAGAAGTGTCAATTTGGCCATGTTACCTTTTCCCTTTACACCTGAGCATTTCACATATTGTGTTACTTGGGATATGGTTTGTTAAAGATCACGTGGGAACTAGGAAGTGAGACCTATGGTTAGAGGGTTTGCTTTTTTTTACTTATTGGTGTTGTGCAGTTTTTCTAACTTCTCTCCATACAACTACCTATTTATTCAAATCTTGAAGTAGTTCACTCCGTTTCTGCCTTTTATTTCCGATTCCTACTTGAATGCTTTAGGTGTCTTTTGGATTGATCTTTTGTAATAATTTGATACGGTGAGCTATGAAGCTAATTTCTCATCTATGGTCTACACATTAACAAGAACGTGAAATATTTGTCTTGACCGATCACGTAATTTACTTTCAAATGTTCTTCGATTTAGGTTAGACTTCATTGCAGTTCATTCTTCCTTTTGTAACGAACCCAAATCAGATAAGAATCAGGCCGGCTGTGCGTTTCACGTACTCCAGAAGAAGACAAAACAAGAAGGGGCAAAAGGAAACTTCTGA

mRNA sequence

TCGGAATTTCTCCTATTTGCCTGCCGCGACTCGAAAAATCAATAATGAACAATTACATGCTGCAACCAGTTTCATTCATTCACAGAGTGACAAAACCCAGAAAAAAAAAATGCTCTGAAATTTAAACCTGCAGCAAAAAATGGGGAAGAACAGCAAGAAGCAGAGGCATCAACAGAAACCTTTAGCTCGTCAAAGAAACTCTAATTTTCCTCCAGAAGATGAACATTACCCTCCTCTTTCTCTCCCCAATAGCGAAGAAGAAACCCCAGAAGAAGAAGAACCTGAACCAGAAATTCACCAAACCCCTTCTTCCGATGATTCAATTCCTTCAAAGTTTTCTCTCTATCAACAATCTGTTCAGTCACCAAAAGGGGATATAAGCTATTTGCAGAAGTTTTTCTTGATGTATGTTGGTGGGAGGGCTCCTCTTCATCTACAGGAGGATTTTTGTGGCACTGCTCTTATTAGGTTAGACTTCATTGCAGTTCATTCTTCCTTTTGTAACGAACCCAAATCAGATAAGAATCAGGCCGGCTGTGCGTTTCACGTACTCCAGAAGAAGACAAAACAAGAAGGGGCAAAAGGAAACTTCTGA

Coding sequence (CDS)

ATGGGGAAGAACAGCAAGAAGCAGAGGCATCAACAGAAACCTTTAGCTCGTCAAAGAAACTCTAATTTTCCTCCAGAAGATGAACATTACCCTCCTCTTTCTCTCCCCAATAGCGAAGAAGAAACCCCAGAAGAAGAAGAACCTGAACCAGAAATTCACCAAACCCCTTCTTCCGATGATTCAATTCCTTCAAAGTTTTCTCTCTATCAACAATCTGTTCAGTCACCAAAAGGGGATATAAGCTATTTGCAGAAGTTTTTCTTGATGTATGTTGGTGGGAGGGCTCCTCTTCATCTACAGGAGGATTTTTGTGGCACTGCTCTTATTAGGTTAGACTTCATTGCAGTTCATTCTTCCTTTTGTAACGAACCCAAATCAGATAAGAATCAGGCCGGCTGTGCGTTTCACGTACTCCAGAAGAAGACAAAACAAGAAGGGGCAAAAGGAAACTTCTGA

Protein sequence

MGKNSKKQRHQQKPLARQRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIHQTPSSDDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDFIAVHSSFCNEPKSDKNQAGCAFHVLQKKTKQEGAKGNF
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo07733Spo07733gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo07733.1Spo07733.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo07733.1.utr5p.1Spo07733.1.utr5p.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo07733.1.CDS.3Spo07733.1.CDS.3CDS
Spo07733.1.CDS.2Spo07733.1.CDS.2CDS
Spo07733.1.CDS.1Spo07733.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo07733.1.exon.3Spo07733.1.exon.3exon
Spo07733.1.exon.2Spo07733.1.exon.2exon
Spo07733.1.exon.1Spo07733.1.exon.1exon


Homology
[Close]
BLAST of Spo07733.1 vs. NCBI nr
Match: gi|902194725|gb|KNA12640.1| (hypothetical protein SOVF_124210 [Spinacia oleracea])

HSP 1 Score: 215.3 bits (547), Expect = 7.300e-53
Identity = 109/114 (95.61%), Postives = 112/114 (98.25%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLARQRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIHQTPSSDD 60
           MGKNSKKQRHQQKPLARQRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIHQTPSSDD
Sbjct: 1   MGKNSKKQRHQQKPLARQRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIHQTPSSDD 60

Query: 61  SIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDFI 115
           SIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALI  +++
Sbjct: 61  SIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALISTEWL 114
[Close]
BLAST of Spo07733.1 vs. NCBI nr
Match: gi|731349524|ref|XP_010686036.1| (PREDICTED: uncharacterized protein LOC104900332 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 152.9 bits (385), Expect = 4.400e-34
Identity = 88/129 (68.22%), Postives = 99/129 (76.74%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLARQRNSNFPPEDEHY----------PPLSLPNSEEETPEEEEPEP 60
           MGKNSKKQ+HQQKP +RQRNS+FPPEDE Y          P LSL NSEEE+ EEEE + 
Sbjct: 1   MGKNSKKQKHQQKPSSRQRNSHFPPEDEDYNFNGCSDQCPPSLSLSNSEEESQEEEENQN 60

Query: 61  EI-----HQTPSSDDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCG 115
           +      H +   DD IPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCG
Sbjct: 61  KSDPQNPHNSFLVDDLIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCG 120
[Close]
BLAST of Spo07733.1 vs. NCBI nr
Match: gi|698482377|ref|XP_009788116.1| (PREDICTED: uncharacterized protein LOC104235978 [Nicotiana sylvestris])

HSP 1 Score: 121.7 bits (304), Expect = 1.100e-24
Identity = 71/117 (60.68%), Postives = 82/117 (70.09%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLARQRNSNFPPEDEHYP---PLSLPNSEEETPEEEEPEPEIHQTPS 60
           MGKN KK+ HQQ    R R      E++ YP   P   P +EEE   +E+  PEI +  S
Sbjct: 1   MGKNGKKRGHQQNRRERDRAYYLEEEEQEYPAALPTEFPEAEEE---QEDENPEIEKE-S 60

Query: 61  SDDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDFI 115
            DD IPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGR PLH QEDFCGTAL+  +++
Sbjct: 61  QDDLIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRVPLHFQEDFCGTALLSTEWL 113
[Close]
BLAST of Spo07733.1 vs. NCBI nr
Match: gi|590586994|ref|XP_007015854.1| (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein isoform 1 [Theobroma cacao])

HSP 1 Score: 117.9 bits (294), Expect = 1.600e-23
Identity = 72/123 (58.54%), Postives = 80/123 (65.04%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLAR-------QRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIH 60
           MGK  KKQRHQQ+   R       Q + ++  EDE Y         EE  +EEE EPE  
Sbjct: 1   MGKRDKKQRHQQRTSQRGASYYTAQHDDDY--EDETYNFQQQSQDSEERQQEEEEEPEEE 60

Query: 61  QTPSSDDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDF 117
             P+    +PSKF LYQQSVQSPKGDISYLQKFFLMYVGGR PLHLQEDFCGTAL+R   
Sbjct: 61  NDPNPSTEMPSKFLLYQQSVQSPKGDISYLQKFFLMYVGGRLPLHLQEDFCGTALLRCKA 120
[Close]
BLAST of Spo07733.1 vs. NCBI nr
Match: gi|590586998|ref|XP_007015855.1| (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein isoform 2 [Theobroma cacao])

HSP 1 Score: 116.7 bits (291), Expect = 3.500e-23
Identity = 70/125 (56.00%), Postives = 81/125 (64.80%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLAR-------QRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIH 60
           MGK  KKQRHQQ+   R       Q + ++  EDE Y         EE  +EEE EPE  
Sbjct: 1   MGKRDKKQRHQQRTSQRGASYYTAQHDDDY--EDETYNFQQQSQDSEERQQEEEEEPEEE 60

Query: 61  QTPSSDDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDF 119
             P+    +PSKF LYQQSVQSPKGDISYLQKFFLMYVGGR PLHLQEDFCGTAL+  ++
Sbjct: 61  NDPNPSTEMPSKFLLYQQSVQSPKGDISYLQKFFLMYVGGRLPLHLQEDFCGTALLSTEW 120
[Close]
BLAST of Spo07733.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QZG3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_124210 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 5.100e-53
Identity = 109/114 (95.61%), Postives = 112/114 (98.25%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLARQRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIHQTPSSDD 60
           MGKNSKKQRHQQKPLARQRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIHQTPSSDD
Sbjct: 1   MGKNSKKQRHQQKPLARQRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIHQTPSSDD 60

Query: 61  SIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDFI 115
           SIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALI  +++
Sbjct: 61  SIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALISTEWL 114
[Close]
BLAST of Spo07733.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C017_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g176270 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.100e-34
Identity = 88/129 (68.22%), Postives = 99/129 (76.74%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLARQRNSNFPPEDEHY----------PPLSLPNSEEETPEEEEPEP 60
           MGKNSKKQ+HQQKP +RQRNS+FPPEDE Y          P LSL NSEEE+ EEEE + 
Sbjct: 1   MGKNSKKQKHQQKPSSRQRNSHFPPEDEDYNFNGCSDQCPPSLSLSNSEEESQEEEENQN 60

Query: 61  EI-----HQTPSSDDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCG 115
           +      H +   DD IPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCG
Sbjct: 61  KSDPQNPHNSFLVDDLIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCG 120
[Close]
BLAST of Spo07733.1 vs. UniProtKB/TrEMBL
Match: A0A061GVL9_THECC (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_041447 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.100e-23
Identity = 72/123 (58.54%), Postives = 80/123 (65.04%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLAR-------QRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIH 60
           MGK  KKQRHQQ+   R       Q + ++  EDE Y         EE  +EEE EPE  
Sbjct: 1   MGKRDKKQRHQQRTSQRGASYYTAQHDDDY--EDETYNFQQQSQDSEERQQEEEEEPEEE 60

Query: 61  QTPSSDDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDF 117
             P+    +PSKF LYQQSVQSPKGDISYLQKFFLMYVGGR PLHLQEDFCGTAL+R   
Sbjct: 61  NDPNPSTEMPSKFLLYQQSVQSPKGDISYLQKFFLMYVGGRLPLHLQEDFCGTALLRCKA 120
[Close]
BLAST of Spo07733.1 vs. UniProtKB/TrEMBL
Match: A0A061GZR1_THECC (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein isoform 2 OS=Theobroma cacao GN=TCM_041447 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.500e-23
Identity = 70/125 (56.00%), Postives = 81/125 (64.80%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLAR-------QRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIH 60
           MGK  KKQRHQQ+   R       Q + ++  EDE Y         EE  +EEE EPE  
Sbjct: 1   MGKRDKKQRHQQRTSQRGASYYTAQHDDDY--EDETYNFQQQSQDSEERQQEEEEEPEEE 60

Query: 61  QTPSSDDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDF 119
             P+    +PSKF LYQQSVQSPKGDISYLQKFFLMYVGGR PLHLQEDFCGTAL+  ++
Sbjct: 61  NDPNPSTEMPSKFLLYQQSVQSPKGDISYLQKFFLMYVGGRLPLHLQEDFCGTALLSTEW 120
[Close]
BLAST of Spo07733.1 vs. UniProtKB/TrEMBL
Match: A0A068UH14_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00023990001 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.100e-22
Identity = 69/116 (59.48%), Postives = 84/116 (72.41%), Query Frame = 1 

		  

Query: 1   MGKNSKK--QRHQQKPLARQRNSNFPPEDEHYPPLSLPNSEEETPEEEEPEPEIHQTPSS 60
           MGK+ KK  Q+ QQK   R++  +   +DE          EEE  EEEE + EI Q+PS+
Sbjct: 1   MGKHGKKLSQQQQQKQQRREQRDSEKNDDESQ------EEEEEEVEEEEEDNEISQSPSN 60

Query: 61  DDSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDFI 115
           +D +PSKF LYQQSVQSPKGDISYLQKFFLMYVGGR PLHLQEDFCGTAL+  +++
Sbjct: 61  ND-VPSKFLLYQQSVQSPKGDISYLQKFFLMYVGGRMPLHLQEDFCGTALLSTEWL 109
[Close]
BLAST of Spo07733.1 vs. TAIR (Arabidopsis)
Match: AT2G27590.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 93.6 bits (231), Expect = 1.100e-19
Identity = 61/115 (53.04%), Postives = 78/115 (67.83%), Query Frame = 1 

		  

Query: 1   MGKNSKKQRHQQKPLARQRNSNFPPEDEHYPPL-SLPNSEEETPEEEEPEPEIHQTPSSD 60
           MGK  KK  ++++      N      D+H P + S+   +EE  +EEE E + ++  SSD
Sbjct: 1   MGKREKKP-NRRRHKGEYSNDAEDFYDQHPPAVASVDEEDEENSDEEEEENDGNE--SSD 60

Query: 61  DSIPSKFSLYQQSVQSPKGDISYLQKFFLMYVGGRAPLHLQEDFCGTALIRLDFI 115
             +PSKF LYQQSVQSPKGDISYLQKFFLMYVGGR P H QEDFCGTAL+  +++
Sbjct: 61  --LPSKFLLYQQSVQSPKGDISYLQKFFLMYVGGRQPFHFQEDFCGTALLSAEWL 110
The following BLAST results are available for this feature:
BLAST of Spo07733.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902194725|gb|KNA12640.1|7.3e-5395.6hypothetical protein SOVF_1242... [more]
gi|731349524|ref|XP_010686036.1|4.4e-3468.2PREDICTED: uncharacterized pro... [more]
gi|698482377|ref|XP_009788116.1|1.1e-2460.6PREDICTED: uncharacterized pro... [more]
gi|590586994|ref|XP_007015854.1|1.6e-2358.5S-adenosyl-L-methionine-depend... [more]
gi|590586998|ref|XP_007015855.1|3.5e-2356.0S-adenosyl-L-methionine-depend... [more]
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BLAST of Spo07733.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QZG3_SPIOL5.1e-5395.6Uncharacterized protein OS=Spi... [more]
A0A0J8C017_BETVU3.1e-3468.2Uncharacterized protein OS=Bet... [more]
A0A061GVL9_THECC1.1e-2358.5S-adenosyl-L-methionine-depend... [more]
A0A061GZR1_THECC2.5e-2356.0S-adenosyl-L-methionine-depend... [more]
A0A068UH14_COFCA2.1e-2259.4Uncharacterized protein OS=Cof... [more]
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BLAST of Spo07733.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo07733.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT2G27590.11.1e-1953.0S-adenosyl-L-methionine-depend... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR37211FAMILY NOT NAMEDcoord: 37..118
score: 2.8

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity