Spo06392.1 (mRNA) | SpinachBase

Overview


Name	Spo06392.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Tonoplast intrinsic protein
Location	SpoScf_00953 : 199211 .. 202850 (-)
Sequence length	1148

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGCCGGTTCACTGTGCTGACGTGGACGGTATGGTGGGAATTAACAAGAAAAGTAACGTATTTTGGGAATTAACTTATCTTTGTGCAATATTGACGGAAATCGCTCATAATGGCAAAACAATAGTGGCCGGTCCTATCAAAAATAAGAAAAATACTGGCCGGTGGAAGGCATATTCTAGAATTTTCTGTCCAATACAAAATTATTAACCAATATTTCTATGTCATATACGGAGTACTCGGTAGTTTTCACATAAAAAATTAATTATAGAAAATGGACAGGCTGGCCAATGGGCAAGCTATCCCAAACATATCAACACATCATTATTCACAACCTGGAAACCGGCAACAACCGGTTACACTACCAACCCTCTCTCTCACTCAGCCTGGATTACTCTACTATAAAGTACGTGCTCCCATCTCTACGAAGAAGACACTCGAAGAAGAAAAAAAAGTGTGACTCAAAAAACATAAATTATTTATTTTCTCCAATAATTATTTATTTATTATTATTAGTTTGAGAAAATGCCTATCAGCAGGATCTCCGTGGGAACCCCGGAGGAAGTCCGGCACCCAAACACCTTAAAGGCGGGATTGGCGGAATTCATCTCCACCCTTATCTTTGTGTTTGCCGGACAAGGTTCAGGGATGGCTTTTGCCAAGCTTACCGCTGGAGCCGCTGCAACTCCAGCAGGCTTGGTGGCTGCCTCTATCGCCCATGGTTTTGCCCTTTTTGTTGCCGTGTCTGTCGGAGCTAACATCTCCGGTGGACATGTTAACCCTGCAGTCACTTTCGGTGCCTTCCTTGGTGGCAACATTTCTCTTCTTAACAGCCTTGTTTACTGGGTTGCTCAACTTGCTGGCTCCTCTGTTGCGTGCTTGTTGCTTAAGTTTGCCACTGGTGGCTTGGTATGTTACTAATTCTTCATTTTATTTCTCTTTTCTTTTACCTTTTTATTTTTCATGTTTTCACTTGCCTTAATCAACATTTTAGGATACTCTCCTCCTAATGGAAAACCGGCCAATAACCAACATAAAATTAATAATCGTAATTTTAATATGGTATCTCCCTTAAAACTACTCCGTATAAAAAAAAGTAACATGTATAAACTATAGAATTAACATACCTAAACTAAAAAAAGTACCTCTTCTAAAATTATAAAAAAAAGTAACATGTCTAAACTATAGAAATAACATACCTAAATTCGCAAGTATGAACTGGTCTCACCATCAGTTGATGATGGGGATAGTCTCATATAAGAATTTGAGCTCGTTTAATTACATCATGTTATTTGTAATTTATGTTTATGTTTATGGGAGCGGGGATATCTTATTTAATTTAGGGGTTATTTGTGTTACTGCTGCAATTAACATACTCACTCCGTTCCATAATGTCCCTCATTTTTCCGTTATGCGCGGTCTTCAATGCACTATTTTAACCGTTAATAGTTTTAATTTTGCATTAGTCAAAATTATAAAAAAAAATGATATTTAGAAAACTTATATTGAAACGACAAGTTAATATTTTTACTCTTAATTATATTATTAATTACGATCAAAAATTTTAAACTTTGACCATATAAATAGTAAATATGAGGAACATTGTGGAACAGAGGGAGTAGATGGTAGAGTTTATGGATATGGAAAGAAATTTGTATACATAGAAATATGAGATTATATGCTGATTTGCTTAGCTAAGATCTGACAATATTAAATATGGGGTACTCCGTATTAATAGGGGAATGATTAATCTCATGGAATCAATGATGAATGGATCAAGTATCTACGTGTCATCTTGTTCTTAGCAAATTCTTCTTGTATTGCCTTTTCAGGAAATACGAATTATTTTTCTTTTTGTTTAAATAATACTATAAGTTTTTTTAAGTAAGAAATTGTCAGTTTGAATTTCTGCACTAAGATTTAATACTTGGTTATCTGCGAAAGCTTTCGGAATTTTTCATTTAAAAGAAGTTTTGCGGACCCCTTCTCTGTTTAAGCTTTTGGAAATTATTGAAACACTCTGTTTATTTTATTAATTGCAAAATTACATCAACTTTTACATCAATTAGAGTACTCGTATAATGCGCAACCGATTTGCTTCAATATGTATTTTACCGATATAAAATTACTTCTTACGTCCCTTAATACTCGCACCGTTTTGACTGAACACGCATGCCGATGCATAACTTTGACCATCAATATCTCTAACTATATATTATAAAAACTTATAAAAATATTAATATTTTGAAAATACATATTAAGATGAAACCAACAATATATTATACAATAACATTTGTTTTCATATACTATAAATAAAATAGGGTCAAAGTGAATTATATAAATAGTGCAAAAAGTCAAAACGGTGCAAGCATTAAGGGACAGAGGGGAGTATTATTTTGAACTTTCAGCGAAACAAACACTTTTTTCTTGTTTAAAATGTAGATTGATGAGTATTAAAGTTGAAATAATGAAAAGTTATAAATTTTGTGGTTCAAATTCAATTGATATTAAAGTAATTACTTTTTGAGAAAAACGTTATAATAAAAAGTTTAATTCCAACATGACACGTGGGTAGTCCTACTCATTTGTATCAACTCACTTTAATAAGTGGATGATATAGAGAAATCTAGAAAACAAAAACGGGGAGTAAAATTTTAGGCTTGTATTATTGGTGTCCTGTTAATTTTTACAGTCAAATTATATTAATTTGGCCAATCATTGGAGGAAATAGTAAGAAAAGCATTCATTGCCTCCACTTATTATATTTTACATTATCTGCATTATACCTTTCCTACCCCAATTCCTACCCCAATAATTGTTGTAGGCCTCAATAATGTAATGATCATTTATATAAACTAGCTTTTGAGCCCGTTTAAAGAACGGACGGTTGTATAGTGGTTATTTAGTCTATCTTTTTAAATTCTAATTTTATTGAGTTTGTGATTTTAAAAAGAATATATGATTTAAATAGAGGTAAATTTTGAAAGTTGAAAGAATACATAAATTAAATAGTGTATTAATATTCTAGAGTTAATCGGGAAGATGGAGTGGAAAATGTTGAATTAATAGATTGGTGAATTGGTGTTGTATGAGGAAGATAGAGCGAAAGAGGCGATTGGAGTTGTATAATATATCAAAAAACAAAGAAAAAATTAAATAAAATACCAAATTGATGGAGGAAATAATGGATGACACGTGTCATCTAATCAAATGTGTTGACACATGTCATCTAATCAAATGTGGGTTTCCTTTTAAAATACTAGGTATAGATTCATTACTCAATTACAACATATAATAGGATAGACAAATAAATGTAGCAAACTATAATCCCAACAAAAGCCATTTAATTAACTTGTTAGATGAACAATATGCATGTTAACATGTATAATTTTGTTATGTAGGAAACATCTGCCTTCGCACTTGCCGAAGACGTGACCGTATGGAATGCCCTAGTCTTTGAGATACACTACAAGAAATTGATTAATTACCAACCGCTAAAATTGGTTGGTAAAACGCGAAAAACGGTTGGTAAAAGATTTTGCGACCGCCAGCGGTCGCAAAAAAGCGGTCAGTTTTCACCTGGACGGTAATTTAGGAAACTCCAACTGGAAGGCGGTCGCTAAAATTTACCGACAGAATTAG

mRNA sequence

AAGGCCGGTTCACTGTGCTGACGTGGACGGTATGGTGGGAATTAACAAGAAAAGTAACGTATTTTGGGAATTAACTTATCTTTGTGCAATATTGACGGAAATCGCTCATAATGGCAAAACAATAGTGGCCGGTCCTATCAAAAATAAGAAAAATACTGGCCGGTGGAAGGCATATTCTAGAATTTTCTGTCCAATACAAAATTATTAACCAATATTTCTATGTCATATACGGAGTACTCGGTAGTTTTCACATAAAAAATTAATTATAGAAAATGGACAGGCTGGCCAATGGGCAAGCTATCCCAAACATATCAACACATCATTATTCACAACCTGGAAACCGGCAACAACCGGTTACACTACCAACCCTCTCTCTCACTCAGCCTGGATTACTCTACTATAAAGTACGTGCTCCCATCTCTACGAAGAAGACACTCGAAGAAGAAAAAAAAGTGTGACTCAAAAAACATAAATTATTTATTTTCTCCAATAATTATTTATTTATTATTATTAGTTTGAGAAAATGCCTATCAGCAGGATCTCCGTGGGAACCCCGGAGGAAGTCCGGCACCCAAACACCTTAAAGGCGGGATTGGCGGAATTCATCTCCACCCTTATCTTTGTGTTTGCCGGACAAGGTTCAGGGATGGCTTTTGCCAAGCTTACCGCTGGAGCCGCTGCAACTCCAGCAGGCTTGGTGGCTGCCTCTATCGCCCATGGTTTTGCCCTTTTTGTTGCCGTGTCTGTCGGAGCTAACATCTCCGGTGGACATGTTAACCCTGCAGTCACTTTCGGTGCCTTCCTTGGTGGCAACATTTCTCTTCTTAACAGCCTTGTTTACTGGGTTGCTCAACTTGCTGGCTCCTCTGTTGCGTGCTTGTTGCTTAAGTTTGCCACTGGTGGCTTGGAAACATCTGCCTTCGCACTTGCCGAAGACGTGACCGTATGGAATGCCCTAGTCTTTGAGATACACTACAAGAAATTGATTAATTACCAACCGCTAAAATTGGTTGGTAAAACGCGAAAAACGGTTGGTAAAAGATTTTGCGACCGCCAGCGGTCGCAAAAAAGCGGTCAGTTTTCACCTGGACGGTAATTTAGGAAACTCCAACTGGAAGGCGGTCGCTAAAATTTACCGACAGAATTAG

Coding sequence (CDS)

ATGCCTATCAGCAGGATCTCCGTGGGAACCCCGGAGGAAGTCCGGCACCCAAACACCTTAAAGGCGGGATTGGCGGAATTCATCTCCACCCTTATCTTTGTGTTTGCCGGACAAGGTTCAGGGATGGCTTTTGCCAAGCTTACCGCTGGAGCCGCTGCAACTCCAGCAGGCTTGGTGGCTGCCTCTATCGCCCATGGTTTTGCCCTTTTTGTTGCCGTGTCTGTCGGAGCTAACATCTCCGGTGGACATGTTAACCCTGCAGTCACTTTCGGTGCCTTCCTTGGTGGCAACATTTCTCTTCTTAACAGCCTTGTTTACTGGGTTGCTCAACTTGCTGGCTCCTCTGTTGCGTGCTTGTTGCTTAAGTTTGCCACTGGTGGCTTGGAAACATCTGCCTTCGCACTTGCCGAAGACGTGACCGTATGGAATGCCCTAGTCTTTGAGATACACTACAAGAAATTGATTAATTACCAACCGCTAAAATTGGTTGGTAAAACGCGAAAAACGGTTGGTAAAAGATTTTGCGACCGCCAGCGGTCGCAAAAAAGCGGTCAGTTTTCACCTGGACGGTAA

Protein sequence

MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVAASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLLLKFATGGLETSAFALAEDVTVWNALVFEIHYKKLINYQPLKLVGKTRKTVGKRFCDRQRSQKSGQFSPGR

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo06392	Spo06392	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo06392.1	Spo06392.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06392.1.utr5p.1	Spo06392.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06392.1.CDS.2	Spo06392.1.CDS.2	CDS
Spo06392.1.CDS.1	Spo06392.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06392.1.utr3p.1	Spo06392.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06392.1.exon.2	Spo06392.1.exon.2	exon
Spo06392.1.exon.1	Spo06392.1.exon.1	exon

Homology

[Close]

BLAST of Spo06392.1 vs. NCBI nr
Match: gi|902081699|gb|KNA03133.1| (hypothetical protein SOVF_212060 [Spinacia oleracea])

HSP 1 Score: 278.1 bits (710), Expect = 1.100e-71
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA
Sbjct: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL
Sbjct: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKFATGGLETSAFALAEDVTVWNALVFEI
Sbjct: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 149

[Close]

BLAST of Spo06392.1 vs. NCBI nr
Match: gi|1622729|gb|AAB17284.1| (tonoplast intrinsic protein [Mesembryanthemum crystallinum])

HSP 1 Score: 244.6 bits (623), Expect = 1.400e-61
Identity = 130/149 (87.25%), Postives = 136/149 (91.28%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI  ISVG  EE  HP TLK  LAEFISTLIFVFAG+GSGMAFAKLTAGAAATPAGLVA
Sbjct: 1   MPIRDISVGRAEEAAHPETLKQALAEFISTLIFVFAGEGSGMAFAKLTAGAAATPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           AS+AH FALFVAVSVGANISGGHVNPAVTFGAF+GGNISLL  +VYW+AQLAGSSVACLL
Sbjct: 61  ASVAHAFALFVAVSVGANISGGHVNPAVTFGAFIGGNISLLRGIVYWIAQLAGSSVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKFATGGLETSAFAL+ DVTVWNALVFEI
Sbjct: 121 LKFATGGLETSAFALSADVTVWNALVFEI 149

[Close]

BLAST of Spo06392.1 vs. NCBI nr
Match: gi|756179870|gb|AJK93570.1| (tonoplast intrinsic protein [Suaeda glauca])

HSP 1 Score: 244.6 bits (623), Expect = 1.400e-61
Identity = 125/149 (83.89%), Postives = 139/149 (93.29%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI+RI+VGT +E RHPNTLKAGLAEFISTLIFV+AGQGSGMAFAKLTAGAA TPAGLVA
Sbjct: 1   MPINRIAVGTADEARHPNTLKAGLAEFISTLIFVYAGQGSGMAFAKLTAGAANTPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           AS+AHGFALFVAV++GANISGGHVNPAVTFGAF+GGNI+LLN +VYW+AQL GS+VACLL
Sbjct: 61  ASVAHGFALFVAVAIGANISGGHVNPAVTFGAFVGGNITLLNGIVYWIAQLLGSTVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKF TGG+ETSAFALA D + WNALV EI
Sbjct: 121 LKFTTGGMETSAFALATDCSTWNALVMEI 149

[Close]

BLAST of Spo06392.1 vs. NCBI nr
Match: gi|145572859|gb|AAT65835.2| (tonoplast intrinsic protein gamma [Salicornia herbacea])

HSP 1 Score: 240.4 bits (612), Expect = 2.700e-60
Identity = 124/149 (83.22%), Postives = 138/149 (92.62%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI+RI+VGT E+ RHPNTLKAGLAEFISTLIFV+AGQGSGMAFAKLTAGAA TPAGLVA
Sbjct: 1   MPINRIAVGTTEDARHPNTLKAGLAEFISTLIFVYAGQGSGMAFAKLTAGAANTPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           A+I+HGFALFVAV+VGANISGGHVNPAVTFGAF+GGNI+LLN +VYW+AQL GS+VACLL
Sbjct: 61  AAISHGFALFVAVAVGANISGGHVNPAVTFGAFIGGNITLLNGIVYWIAQLLGSTVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKF+TGGL+TSAFAL E  TVW A V EI
Sbjct: 121 LKFSTGGLQTSAFALCEGETVWGAFVMEI 149

[Close]

BLAST of Spo06392.1 vs. NCBI nr
Match: gi|731368735|ref|XP_010696310.1| (PREDICTED: probable aquaporin TIP1-2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 240.0 bits (611), Expect = 3.500e-60
Identity = 125/149 (83.89%), Postives = 136/149 (91.28%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPISRISVGTP+E RHP+TL++GLAEFISTLIFVFAGQGSGMAFAKLT GAA TPAGLVA
Sbjct: 1   MPISRISVGTPDEARHPDTLRSGLAEFISTLIFVFAGQGSGMAFAKLTEGAANTPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           ASIAH FALFVAV+VGANISGGHVNPAVTFGAF+GGNI+LLN +VYW+AQL GS  AC L
Sbjct: 61  ASIAHAFALFVAVAVGANISGGHVNPAVTFGAFVGGNITLLNGIVYWIAQLLGSVAACAL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKFATGGLET+AFA A DV+ WNALV EI
Sbjct: 121 LKFATGGLETAAFACATDVSAWNALVMEI 149

[Close]

BLAST of Spo06392.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q8W8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_212060 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 8.000e-72
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA
Sbjct: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL
Sbjct: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKFATGGLETSAFALAEDVTVWNALVFEI
Sbjct: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 149

[Close]

BLAST of Spo06392.1 vs. UniProtKB/TrEMBL
Match: A0A0C5AZ88_9CARY (Suaeda glauca tonoplast intrinsic protein mRNA OS=Suaeda glauca PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 9.800e-62
Identity = 125/149 (83.89%), Postives = 139/149 (93.29%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI+RI+VGT +E RHPNTLKAGLAEFISTLIFV+AGQGSGMAFAKLTAGAA TPAGLVA
Sbjct: 1   MPINRIAVGTADEARHPNTLKAGLAEFISTLIFVYAGQGSGMAFAKLTAGAANTPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           AS+AHGFALFVAV++GANISGGHVNPAVTFGAF+GGNI+LLN +VYW+AQL GS+VACLL
Sbjct: 61  ASVAHGFALFVAVAIGANISGGHVNPAVTFGAFVGGNITLLNGIVYWIAQLLGSTVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKF TGG+ETSAFALA D + WNALV EI
Sbjct: 121 LKFTTGGMETSAFALATDCSTWNALVMEI 149

[Close]

BLAST of Spo06392.1 vs. UniProtKB/TrEMBL
Match: O24048_MESCR (Tonoplast intrinsic protein OS=Mesembryanthemum crystallinum GN=TIP PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 9.800e-62
Identity = 130/149 (87.25%), Postives = 136/149 (91.28%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI  ISVG  EE  HP TLK  LAEFISTLIFVFAG+GSGMAFAKLTAGAAATPAGLVA
Sbjct: 1   MPIRDISVGRAEEAAHPETLKQALAEFISTLIFVFAGEGSGMAFAKLTAGAAATPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           AS+AH FALFVAVSVGANISGGHVNPAVTFGAF+GGNISLL  +VYW+AQLAGSSVACLL
Sbjct: 61  ASVAHAFALFVAVSVGANISGGHVNPAVTFGAFIGGNISLLRGIVYWIAQLAGSSVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKFATGGLETSAFAL+ DVTVWNALVFEI
Sbjct: 121 LKFATGGLETSAFALSADVTVWNALVFEI 149

[Close]

BLAST of Spo06392.1 vs. UniProtKB/TrEMBL
Match: Q6DW15_9CARY (Tonoplast intrinsic protein gamma OS=Salicornia herbacea PE=2 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 1.900e-60
Identity = 124/149 (83.22%), Postives = 138/149 (92.62%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI+RI+VGT E+ RHPNTLKAGLAEFISTLIFV+AGQGSGMAFAKLTAGAA TPAGLVA
Sbjct: 1   MPINRIAVGTTEDARHPNTLKAGLAEFISTLIFVYAGQGSGMAFAKLTAGAANTPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           A+I+HGFALFVAV+VGANISGGHVNPAVTFGAF+GGNI+LLN +VYW+AQL GS+VACLL
Sbjct: 61  AAISHGFALFVAVAVGANISGGHVNPAVTFGAFIGGNITLLNGIVYWIAQLLGSTVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKF+TGGL+TSAFAL E  TVW A V EI
Sbjct: 121 LKFSTGGLQTSAFALCEGETVWGAFVMEI 149

[Close]

BLAST of Spo06392.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E1T9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g179210 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 2.400e-60
Identity = 125/149 (83.89%), Postives = 136/149 (91.28%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPISRISVGTP+E RHP+TL++GLAEFISTLIFVFAGQGSGMAFAKLT GAA TPAGLVA
Sbjct: 1   MPISRISVGTPDEARHPDTLRSGLAEFISTLIFVFAGQGSGMAFAKLTEGAANTPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           ASIAH FALFVAV+VGANISGGHVNPAVTFGAF+GGNI+LLN +VYW+AQL GS  AC L
Sbjct: 61  ASIAHAFALFVAVAVGANISGGHVNPAVTFGAFVGGNITLLNGIVYWIAQLLGSVAACAL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKFATGGLET+AFA A DV+ WNALV EI
Sbjct: 121 LKFATGGLETAAFACATDVSAWNALVMEI 149

[Close]

BLAST of Spo06392.1 vs. ExPASy Swiss-Prot
Match: TIP12_MAIZE (Aquaporin TIP1-2 OS=Zea mays GN=TIP1-2 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 7.000e-53
Identity = 111/149 (74.50%), Postives = 125/149 (83.89%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MP+SRI+VG P E+ HP+T KA +AEFISTLIFVFAG GSGMAF+KLT G AATPAGL+A
Sbjct: 1   MPVSRIAVGAPGELSHPDTAKAAVAEFISTLIFVFAGSGSGMAFSKLTDGGAATPAGLIA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           AS+AH  ALFVAVSVGANISGGHVNPAVTFGAF+GGNISLL +LVYWVAQL GS VACLL
Sbjct: 61  ASLAHALALFVAVSVGANISGGHVNPAVTFGAFVGGNISLLKALVYWVAQLLGSVVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LK ATGG    AF+L+  V   NA+V E+
Sbjct: 121 LKIATGGAALGAFSLSAGVGAMNAVVLEM 149

[Close]

BLAST of Spo06392.1 vs. ExPASy Swiss-Prot
Match: TIP11_ORYSJ (Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.200e-52
Identity = 109/149 (73.15%), Postives = 124/149 (83.22%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI  I+VG+ +EV HP  LKA LAEFISTLIFVFAGQGSGMAF+KLT G A TPAGL+A
Sbjct: 1   MPIRNIAVGSHQEVYHPGALKAALAEFISTLIFVFAGQGSGMAFSKLTGGGATTPAGLIA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           A++AH FALFVAVSVGANISGGHVNPAVTFGAF+GGNI+L   L+YW+AQL GS+VAC L
Sbjct: 61  AAVAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLFRGLLYWIAQLLGSTVACFL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           L+F+TGGL T  F L   V+VW ALV EI
Sbjct: 121 LRFSTGGLATGTFGLT-GVSVWEALVLEI 148

[Close]

BLAST of Spo06392.1 vs. ExPASy Swiss-Prot
Match: TIP12_ORYSJ (Probable aquaporin TIP1-2 OS=Oryza sativa subsp. japonica GN=TIP1-2 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.000e-52
Identity = 107/149 (71.81%), Postives = 124/149 (83.22%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MP+SRI+VG P E+ HP+T KA +AEFIS LIFVFAG GSGMAF+KLT G   TP+GL+A
Sbjct: 1   MPVSRIAVGAPGELSHPDTAKAAVAEFISMLIFVFAGSGSGMAFSKLTDGGGTTPSGLIA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           AS+AH  ALFVAV+VGANISGGHVNPAVTFGAF+GGNISL+ ++VYWVAQL GS VACLL
Sbjct: 61  ASLAHALALFVAVAVGANISGGHVNPAVTFGAFVGGNISLVKAVVYWVAQLLGSVVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LK ATGG    AF+L+  V  WNA+VFEI
Sbjct: 121 LKIATGGAAVGAFSLSAGVGAWNAVVFEI 149

[Close]

BLAST of Spo06392.1 vs. ExPASy Swiss-Prot
Match: TIP13_ARATH (Aquaporin TIP1-3 OS=Arabidopsis thaliana GN=TIP1-3 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.500e-52
Identity = 103/149 (69.13%), Postives = 127/149 (85.23%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI+RI++GTP E   P+ ++A  AEF S +IFVFAGQGSGMA+ KLT    ATPAGLVA
Sbjct: 1   MPINRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGSGMAYGKLTGDGPATPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           AS++H FALFVAVSVGAN+SGGHVNPAVTFGAF+GGNI+LL +++YW+AQL G+ VACLL
Sbjct: 61  ASLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIGGNITLLRAILYWIAQLLGAVVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LK +TGG+ET+AF+L+  VT WNA+VFEI
Sbjct: 121 LKVSTGGMETAAFSLSYGVTPWNAVVFEI 149

[Close]

BLAST of Spo06392.1 vs. ExPASy Swiss-Prot
Match: TIP11_MAIZE (Aquaporin TIP1-1 OS=Zea mays GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.700e-51
Identity = 107/149 (71.81%), Postives = 123/149 (82.55%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI+RI++G+ +EV HP  LKA  AEFISTLIFVFAGQGSGMAF+KLT G   TPAGL+A
Sbjct: 1   MPINRIALGSHQEVYHPGALKAAFAEFISTLIFVFAGQGSGMAFSKLTGGGPTTPAGLIA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           A++AH FALFVAVSVGANISGGHVNPAVTFGAF+GGNI+L   L+YWVAQL GS+VAC L
Sbjct: 61  AAVAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLFRGLLYWVAQLLGSTVACFL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           L+F+TGG  T  F L   V+VW ALV EI
Sbjct: 121 LRFSTGGQATGTFGLT-GVSVWEALVLEI 148

[Close]

BLAST of Spo06392.1 vs. TAIR (Arabidopsis)
Match: AT4G01470.1 (tonoplast intrinsic protein 1;3)

HSP 1 Score: 206.1 bits (523), Expect = 2.000e-53
Identity = 103/149 (69.13%), Postives = 127/149 (85.23%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI+RI++GTP E   P+ ++A  AEF S +IFVFAGQGSGMA+ KLT    ATPAGLVA
Sbjct: 1   MPINRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGSGMAYGKLTGDGPATPAGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           AS++H FALFVAVSVGAN+SGGHVNPAVTFGAF+GGNI+LL +++YW+AQL G+ VACLL
Sbjct: 61  ASLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIGGNITLLRAILYWIAQLLGAVVACLL 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LK +TGG+ET+AF+L+  VT WNA+VFEI
Sbjct: 121 LKVSTGGMETAAFSLSYGVTPWNAVVFEI 149

[Close]

BLAST of Spo06392.1 vs. TAIR (Arabidopsis)
Match: AT2G36830.1 (gamma tonoplast intrinsic protein)

HSP 1 Score: 199.1 bits (505), Expect = 2.400e-51
Identity = 105/149 (70.47%), Postives = 119/149 (79.87%), Query Frame = 1


		  
Query: 1   MPISRISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVA 60
           MPI  I++G P+E   P+ LKA LAEFISTLIFV AG GSGMAF KLT   A TP+GLVA
Sbjct: 1   MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKLTENGATTPSGLVA 60

Query: 61  ASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLL 120
           A++AH F LFVAVSVGANISGGHVNPAVTFGAF+GGNI+LL  ++YW+AQL GS VACL+
Sbjct: 61  AAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI 120

Query: 121 LKFATGGLETSAFALAEDVTVWNALVFEI 150
           LKFATGGL   AF L+  V V NA VFEI
Sbjct: 121 LKFATGGLAVPAFGLSAGVGVLNAFVFEI 149

[Close]

BLAST of Spo06392.1 vs. TAIR (Arabidopsis)
Match: AT3G26520.1 (tonoplast intrinsic protein 2)

HSP 1 Score: 184.5 bits (467), Expect = 6.100e-47
Identity = 101/150 (67.33%), Postives = 114/150 (76.00%), Query Frame = 1


		  
Query: 1   MPISRISVG-TPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLV 60
           MP   I++G   EEV HPN L+A LAEFISTLIFVFAG GSG+AF K+T   A TP+GLV
Sbjct: 1   MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKITDNGATTPSGLV 60

Query: 61  AASIAHGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACL 120
           AA++AH F LFVAVSVGANISGGHVNPAVTFG  LGGNI+LL  ++YW+AQL GS  AC 
Sbjct: 61  AAALAHAFGLFVAVSVGANISGGHVNPAVTFGVLLGGNITLLRGILYWIAQLLGSVAACF 120

Query: 121 LLKFATGGLETSAFALAEDVTVWNALVFEI 150
           LL FATGG    AF L+  V   NALVFEI
Sbjct: 121 LLSFATGGEPIPAFGLSAGVGSLNALVFEI 150

[Close]

BLAST of Spo06392.1 vs. TAIR (Arabidopsis)
Match: AT3G16240.1 (delta tonoplast integral protein)

HSP 1 Score: 142.1 bits (357), Expect = 3.500e-34
Identity = 76/131 (58.02%), Postives = 98/131 (74.81%), Query Frame = 1


		  
Query: 19  TLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVAASIAHGFALFVAVSVGAN 78
           +L+A LAEFISTL+FVFAG GS +A+AKLT+ AA    GLVA ++ HGFALFVAV++GAN
Sbjct: 17  SLRAYLAEFISTLLFVFAGVGSAIAYAKLTSDAALDTPGLVAIAVCHGFALFVAVAIGAN 76

Query: 79  ISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLLLKFATGGLETSAFALAED 138
           ISGGHVNPAVTFG  +GG I+++  + YW+AQL GS+ AC LLK+ TGGL     ++A  
Sbjct: 77  ISGGHVNPAVTFGLAVGGQITVITGVFYWIAQLLGSTAACFLLKYVTGGLAVPTHSVAAG 136

Query: 139 VTVWNALVFEI 150
           +     +V EI
Sbjct: 137 LGSIEGVVMEI 147

[Close]

BLAST of Spo06392.1 vs. TAIR (Arabidopsis)
Match: AT5G47450.1 (tonoplast intrinsic protein 2;3)

HSP 1 Score: 139.8 bits (351), Expect = 1.700e-33
Identity = 78/145 (53.79%), Postives = 99/145 (68.28%), Query Frame = 1


		  
Query: 5   RISVGTPEEVRHPNTLKAGLAEFISTLIFVFAGQGSGMAFAKLTAGAAATPAGLVAASIA 64
           +I VG+  +    ++LKA L+EFI+TL+FVFAG GS +AFAKLT+  A  PAGLVA +IA
Sbjct: 3   KIEVGSVGDSFSVSSLKAYLSEFIATLLFVFAGVGSAVAFAKLTSDGALDPAGLVAIAIA 62

Query: 65  HGFALFVAVSVGANISGGHVNPAVTFGAFLGGNISLLNSLVYWVAQLAGSSVACLLLKFA 124
           H FALFV VS+ ANISGGH+NPAVT G  +GGNI+L+    YW+AQ  GS VACLLL F 
Sbjct: 63  HAFALFVGVSIAANISGGHLNPAVTLGLAIGGNITLITGFFYWIAQCLGSIVACLLLVFV 122

Query: 125 TGGLETSAFALAEDVTVWNALVFEI 150
           T G       ++  +     +V EI
Sbjct: 123 TNGKSVPTHGVSAGLGAVEGVVMEI 147

The following BLAST results are available for this feature:

BLAST of Spo06392.1 vs. NCBI nr
BLAST of Spo06392.1 vs. UniProtKB/TrEMBL
BLAST of Spo06392.1 vs. ExPASy Swiss-Prot
BLAST of Spo06392.1 vs. TAIR (Arabidopsis)

BLAST of Spo06392.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902081699\|gb\|KNA03133.1\|	1.1e-71	100.	hypothetical protein SOVF_2120...	[more]
gi\|1622729\|gb\|AAB17284.1\|	1.4e-61	87.2	tonoplast intrinsic protein [M...	[more]
gi\|756179870\|gb\|AJK93570.1\|	1.4e-61	83.8	tonoplast intrinsic protein [S...	[more]
gi\|145572859\|gb\|AAT65835.2\|	2.7e-60	83.2	tonoplast intrinsic protein ga...	[more]
gi\|731368735\|ref\|XP_010696310.1\|	3.5e-60	83.8	PREDICTED: probable aquaporin ...	[more]

BLAST of Spo06392.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q8W8_SPIOL	8.0e-72	100.	Uncharacterized protein OS=Spi...	[more]
A0A0C5AZ88_9CARY	9.8e-62	83.8	Suaeda glauca tonoplast intrin...	[more]
O24048_MESCR	9.8e-62	87.2	Tonoplast intrinsic protein OS...	[more]
Q6DW15_9CARY	1.9e-60	83.2	Tonoplast intrinsic protein ga...	[more]
A0A0J8E1T9_BETVU	2.4e-60	83.8	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo06392.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
TIP12_MAIZE	7.0e-53	74.5	Aquaporin TIP1-2 OS=Zea mays G...	[more]
TIP11_ORYSJ	1.2e-52	73.1	Probable aquaporin TIP1-1 OS=O...	[more]
TIP12_ORYSJ	2.0e-52	71.8	Probable aquaporin TIP1-2 OS=O...	[more]
TIP13_ARATH	3.5e-52	69.1	Aquaporin TIP1-3 OS=Arabidopsi...	[more]
TIP11_MAIZE	1.7e-51	71.8	Aquaporin TIP1-1 OS=Zea mays G...	[more]

BLAST of Spo06392.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G01470.1	2.0e-53	69.1	tonoplast intrinsic protein 1;...	[more]
AT2G36830.1	2.4e-51	70.4	gamma tonoplast intrinsic prot...	[more]
AT3G26520.1	6.1e-47	67.3	tonoplast intrinsic protein 2	[more]
AT3G16240.1	3.5e-34	58.0	delta tonoplast integral prote...	[more]
AT5G47450.1	1.7e-33	53.7	tonoplast intrinsic protein 2;...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 65..89 score: 4.3E-24coord: 21..40 score: 4.3E-24coord: 102..121 score: 4.3
IPR000425	Major intrinsic protein	PANTHER	PTHR19139	AQUAPORIN TRANSPORTER	coord: 3..149 score: 2.4
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 14..152 score: 6.4
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 83..91 scor
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10		coord: 13..149 score: 4.7
IPR023271	Aquaporin-like	unknown	SSF81338	Aquaporin-like	coord: 13..150 score: 3.01
None	No IPR available	PANTHER	PTHR19139:SF52	AQUAPORIN TIP1-1	coord: 3..149 score: 2.4

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006810	transport
biological_process	GO:0006950	response to stress
biological_process	GO:0071918	urea transmembrane transport
biological_process	GO:0006833	water transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005774	vacuolar membrane
molecular_function	GO:0005215	transporter activity
molecular_function	GO:0015204	urea transmembrane transporter activity
molecular_function	GO:0015250	water channel activity