Spo05029.1 (mRNA) | SpinachBase

Overview


Name	Spo05029.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	rRNA intron-encoded homing endonuclease
Location	SpoScf_40346 : 369 .. 932 (+)
Sequence length	426

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCTGGTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTCCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCT

mRNA sequence

AAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTCCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCT

Coding sequence (CDS)

AAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTCCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCT

Protein sequence

KKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGGSMVARLKLKGIDGRAPPGVEPAA

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo05029	Spo05029	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo05029.1	Spo05029.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo05029.1.exon.1	Spo05029.1.exon.1	exon
Spo05029.1.exon.2	Spo05029.1.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo05029.1.CDS.1	Spo05029.1.CDS.1	CDS
Spo05029.1.CDS.2	Spo05029.1.CDS.2	CDS

Homology

[Close]

BLAST of Spo05029.1 vs. NCBI nr
Match: gi|703137890|ref|XP_010106597.1| (hypothetical protein L484_002552 [Morus notabilis])

HSP 1 Score: 225.7 bits (574), Expect = 5.100e-56
Identity = 118/138 (85.51%), Postives = 119/138 (86.23%), Query Frame = 1


		  
Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYAL------------RTTAKAF 76
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYAL            RTTAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 77  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGGSMVAR 136
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSKFLG GGSMVAR
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGSMVAR 174

Query: 137 LKLKGIDGRAPPGVEPAA 143
           LKLKGIDGRAPPGVEPAA
Sbjct: 175 LKLKGIDGRAPPGVEPAA 192

[Close]

BLAST of Spo05029.1 vs. NCBI nr
Match: gi|823127874|ref|XP_012440607.1| (PREDICTED: uncharacterized protein LOC105765883 [Gossypium raimondii])

HSP 1 Score: 225.7 bits (574), Expect = 5.100e-56
Identity = 115/139 (82.73%), Postives = 120/139 (86.33%), Query Frame = 1


		  
Query: 1   KKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALRTT 60
           KKLVVG  GG+ GPP G HR +R + RRCAPGLNWPGR+S  VTLKKLECSKQAYA    
Sbjct: 41  KKLVVGLRGGSAGPPHGEHRSARPYCRRCAPGLNWPGRSSDAVTLKKLECSKQAYA---- 100

Query: 61  AKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGGS 120
             +FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYEKSK LG GGS
Sbjct: 101 --SFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKSLGSGGS 160

Query: 121 MVARLKLKGIDGRAPPGVE 140
           MVARLKLKGIDGRAPPGVE
Sbjct: 161 MVARLKLKGIDGRAPPGVE 173

[Close]

BLAST of Spo05029.1 vs. NCBI nr
Match: gi|694380941|ref|XP_009366572.1| (PREDICTED: uncharacterized protein LOC103956333 [Pyrus x bretschneideri])

HSP 1 Score: 215.7 bits (548), Expect = 5.200e-53
Identity = 114/142 (80.28%), Postives = 116/142 (81.69%), Query Frame = 1


		  
Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYAL-----------------RT 76
           GVHR +R F RR APGLNWPGRASG VTLKKLECSKQ +                   RT
Sbjct: 228 GVHRSARPFCRRYAPGLNWPGRASGAVTLKKLECSKQDFGPILLAFGIGVMINRDSRGRT 287

Query: 77  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGG 136
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLG GG
Sbjct: 288 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG 347

Query: 137 SMVARLKLKGIDGRAPPGVEPA 142
           SMVARLKLKGIDGRAPPGVEPA
Sbjct: 348 SMVARLKLKGIDGRAPPGVEPA 369

[Close]

BLAST of Spo05029.1 vs. NCBI nr
Match: gi|823218726|ref|XP_012442053.1| (PREDICTED: uncharacterized protein LOC105767074 [Gossypium raimondii])

HSP 1 Score: 214.2 bits (544), Expect = 1.500e-52
Identity = 108/135 (80.00%), Postives = 112/135 (82.96%), Query Frame = 1


		  
Query: 8   WGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALRTTAKAFAKD 67
           W G+ G P G H+  R + RRCAPGLNWPGR+SG VTLKKLECSKQAYA       FAKD
Sbjct: 36  WAGSAGLPHGEHQSVRPYCRRCAPGLNWPGRSSGAVTLKKLECSKQAYA------TFAKD 95

Query: 68  VFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGGSMVARLKL 127
           VFINQERKLGARRRSDTV VSTINDADQGS DV FRTPP PYEKSK LG GGSMVARLKL
Sbjct: 96  VFINQERKLGARRRSDTVPVSTINDADQGSTDVAFRTPPVPYEKSKSLGSGGSMVARLKL 155

Query: 128 KGIDGRAPPGVEPAA 143
           KGIDGRAPPGVEPAA
Sbjct: 156 KGIDGRAPPGVEPAA 164

[Close]

BLAST of Spo05029.1 vs. NCBI nr
Match: gi|922327155|ref|XP_013442970.1| (hypothetical protein MTR_0055s0130 [Medicago truncatula])

HSP 1 Score: 211.8 bits (538), Expect = 7.600e-52
Identity = 113/143 (79.02%), Postives = 117/143 (81.82%), Query Frame = 1


		  
Query: 16  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQ-------AYAL----------R 75
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ       A+ +          R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 76  TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPG 135
           TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK LG G
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 604

Query: 136 GSMVARLKLKGIDGRAPPGVEPA 142
           GSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 GSMVARLKLKGIDGRAPPGVEPA 627

[Close]

BLAST of Spo05029.1 vs. UniProtKB/TrEMBL
Match: M1DUP9_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400044276 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 5.400e-57
Identity = 120/141 (85.11%), Postives = 120/141 (85.11%), Query Frame = 1


		  
Query: 14  PPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYAL------------RTTA 73
           P   VHR SR F RRCAPGLNWPGRASG VTLKKLECSKQAYAL            RTTA
Sbjct: 45  PCICVHRSSRPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTA 104

Query: 74  KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGGSM 133
           KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYEKSKFLG GGSM
Sbjct: 105 KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLGSGGSM 164

Query: 134 VARLKLKGIDGRAPPGVEPAA 143
           VARLKLKGIDGRAPPGVEPAA
Sbjct: 165 VARLKLKGIDGRAPPGVEPAA 185

[Close]

BLAST of Spo05029.1 vs. UniProtKB/TrEMBL
Match: W9SJ11_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_002552 PE=4 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 3.500e-56
Identity = 118/138 (85.51%), Postives = 119/138 (86.23%), Query Frame = 1


		  
Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYAL------------RTTAKAF 76
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYAL            RTTAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 77  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGGSMVAR 136
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSKFLG GGSMVAR
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGSMVAR 174

Query: 137 LKLKGIDGRAPPGVEPAA 143
           LKLKGIDGRAPPGVEPAA
Sbjct: 175 LKLKGIDGRAPPGVEPAA 192

[Close]

BLAST of Spo05029.1 vs. UniProtKB/TrEMBL
Match: M1DB49_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400035737 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 3.100e-52
Identity = 111/130 (85.38%), Postives = 113/130 (86.92%), Query Frame = 1


		  
Query: 25  FRRRCAPGLNWPGRASGTVTLKKLECSKQAYAL------------RTTAKAFAKDVFINQ 84
           F RRCAPGLNWPGRASG VTLKKLECSKQAYAL            RTTAKAFAKDVFINQ
Sbjct: 5   FCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAFAKDVFINQ 64

Query: 85  ERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGGSMVARLKLKGIDG 143
           ERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYEKSKFLG GGS+VARLKLKGIDG
Sbjct: 65  ERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLGSGGSIVARLKLKGIDG 124

[Close]

BLAST of Spo05029.1 vs. UniProtKB/TrEMBL
Match: A0A072TTW7_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_0055s0130 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 5.300e-52
Identity = 113/143 (79.02%), Postives = 117/143 (81.82%), Query Frame = 1


		  
Query: 16  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQ-------AYAL----------R 75
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ       A+ +          R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 76  TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPG 135
           TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK LG G
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 604

Query: 136 GSMVARLKLKGIDGRAPPGVEPA 142
           GSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 GSMVARLKLKGIDGRAPPGVEPA 627

[Close]

BLAST of Spo05029.1 vs. UniProtKB/TrEMBL
Match: A0A0R0GPK3_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_13G016700 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 3.400e-51
Identity = 113/143 (79.02%), Postives = 116/143 (81.12%), Query Frame = 1


		  
Query: 17  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQ-------AYAL----------RT 76
           GVHR +R F RRCAP LNWPGRASG VTLKKLECSKQ       A+ +          RT
Sbjct: 128 GVHRSARPFCRRCAPVLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGRT 187

Query: 77  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGPGG 136
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK LG GG
Sbjct: 188 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGG 247

Query: 137 SMVARLKLKGIDGRAPPGVEPAA 143
           SMVARLKLKGIDGRAPPGVEPAA
Sbjct: 248 SMVARLKLKGIDGRAPPGVEPAA 270

The following BLAST results are available for this feature:

BLAST of Spo05029.1 vs. NCBI nr
BLAST of Spo05029.1 vs. UniProtKB/TrEMBL
BLAST of Spo05029.1 vs. ExPASy Swiss-Prot
BLAST of Spo05029.1 vs. TAIR (Arabidopsis)

BLAST of Spo05029.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|703137890\|ref\|XP_010106597.1\|	5.1e-56	85.5	hypothetical protein L484_0025...	[more]
gi\|823127874\|ref\|XP_012440607.1\|	5.1e-56	82.7	PREDICTED: uncharacterized pro...	[more]
gi\|694380941\|ref\|XP_009366572.1\|	5.2e-53	80.2	PREDICTED: uncharacterized pro...	[more]
gi\|823218726\|ref\|XP_012442053.1\|	1.5e-52	80.0	PREDICTED: uncharacterized pro...	[more]
gi\|922327155\|ref\|XP_013442970.1\|	7.6e-52	79.0	hypothetical protein MTR_0055s...	[more]

BLAST of Spo05029.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
M1DUP9_SOLTU	5.4e-57	85.1	Uncharacterized protein OS=Sol...	[more]
W9SJ11_9ROSA	3.5e-56	85.5	Uncharacterized protein OS=Mor...	[more]
M1DB49_SOLTU	3.1e-52	85.3	Uncharacterized protein OS=Sol...	[more]
A0A072TTW7_MEDTR	5.3e-52	79.0	Uncharacterized protein OS=Med...	[more]
A0A0R0GPK3_SOYBN	3.4e-51	79.0	Uncharacterized protein OS=Gly...	[more]

BLAST of Spo05029.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo05029.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34410	FAMILY NOT NAMED	coord: 58..142 score: 7.0

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016758	transferase activity, transferring hexosyl groups