Spo02373.1 (mRNA) | SpinachBase

Overview


Name	Spo02373.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	DNA-directed RNA polymerase (2.7.7.6)
Location	chr5 : 2741217 .. 2744823 (+)
Sequence length	1393

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTGAATCCTCGAAGAATTGAAATAGAGAGAGAAGTTTGTGAAGGAAACAGGATCGAATCCTCTATGGCAGTTGCAGTAAGCAGATTCTACAGCAGCTGTACATGATGGTGATGCAGAAGCAGCAACAAGATGGTGAGGGGGCATTTTTGTAATTGATTAGTGATTACTTATTTTTCGGACAGTAAAAACAGAACAAAGTGCGAGCATATCCATTGATATGGAGTGTTATCTGCATAGATTCATATACGCGTTACTGTTATTAATGTATACGTGTGTTCTTTTCTCTTCTTTGAGCACTTATGAATTGTGTAGAATGAAGTTGTAGGAACACATTTTTTGACACAGAAGTGTCCTGTTATAAGAATGTGAAGTTTGTCTTGTCTGAACTTTCATTGATTTATAGCAAATCCTGTGTTATTACATTGTATAGTGCAAGGATGAATATGAACATTGTATGAAGTATACTGTAATAAAGAATGTGAAGGTTGTCTGAACTTTCATTGATTTGTTGCAAATCCTGTGTTATATTACTTTGTTTTGTGTAAGGATGAATTCATTGGACAATGTGCTTTGTACTTTCTCTGTTTTTCACTTGCAATTTGATACTCCCTCTGTTTCTTTTTGTTTGTTACGTATTCCTTTTAGGGTGTTTCACAATGTTTATTACATGGGAGAATATTTCATTTTATAAACTTATTATATTGTCATTTTTTGTGCCAACTTTTAATTTTAATTGGTCCAATTTTTTCAATTCATTAAATTTCTTTACAATTTCCCAAGTATGTTAAGTTAACAATTTGTGTGCTTTGGTTCATTTTGATAAATAAAACAATCTTATTGGTTGTTGTAATGATTAATGGATTGAGGTTTTGCTTGGGTTTATTTTTTTAATAAAAAAGATAAAGAATCTTTATTATACGTTAATAAACGTGCAAAAGTCTAAACGTAACAAACAAAAAGAAACGGAGGGAATACTATTAATGGTTAAAGATGTGCATTGACAAACGTAAAACTCCAAATAGTGCAATTATAAAAATGATGATGCATTTTCTTTGTTTCTTTTGCTGATGGATAACATGGATTGGTAATCGAACCGCTTAGTTTAATAGAACGACCTGGTTAATCGACCATGGTAACACCCCTAGTCTCATTATTTAATGCTTCACCAATTCTCTTTTAATTACGACATTATGACAAAAACAAGTTATGATACTTCGTATTATTTATCTAAATTTATTTTGGTTGAATATTGAAACAATTTCTAGTATGTTACGAGTATTATTTTAGTTTACTTTTAGGAAACAAGTTTAAGTTTGTTAAGAAAAAATAAACTCCTGTAAAATAAGTTTCAAAATGATTCCTAATTGTACATTAATACTACCGTTATTAACGTATTAGATAATATAAATTCAGATAAAATAAATCAAATAAACAACAGACGCGAGTACATGGTAACACGTGGCATCTTTCTTTTAAGTACTCCGTAGATTTTCTGATTTGAGAAGACAAACAATTGAACAAACAAAACAAAGGTTGTTTCAGTTAAAACAACAATGGAGGTTTCATGTTTTCCAGCCACATCAAAAGGAACTCACTCCCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTCCTCTAAATTTTGTTACAAGGCTGAGATTTTATTTTGTGTATGTGGCAGATCATGGTTAAGCGCCAGAGAACAGCATTTCCTCCAAATTTTGTCCACTCTCTTGATGGTTCCCACATGATGATGACTGCCGTTGCCTGCAAACAAGCTGGCATGTACTTTGCAGGTCTGTTTTGTATGCTCAACTTGGATCATCCTATCTCCAGGGGATTGTTGCTTATCTCATTCTAACTATTTGGTCCGCTCACTTGACTTCTATAGGTGTTCATGATTCATATTGGACACATGCATGTGATGTTGACGAAATGAACCGGATATTAAGGGTGAAGTTTGTTGAGCTCTATGAACAACCAATACTTGAGAATGTAAGTATTTTTATATGATGAGTACTCTTTTGGTCCATGAAGTTTTGTTTATCCACTAAAACCTTTTGTTTCTTTTTCTCCGTTTATTTTGGGTCTGTTTGAGCTGGAAAAGTCTCTTTTTTGGAGACTCAACTTTTTCCTTGCCTTAGTACTGTACTAAATATAAACTCTACACAATTAGGGTTCAACACACATGAACCCCTTGAAATTGAGCTTAGGCCGAGCTTTAGGCTCTATTTTGCTCACCTTATTTCACTTATTTCAGGAAAAATAAGTTTAGGAAAAATAAGGGTTGACCAAGTACAATTTATAACTAAAGTTTTGATAAGTTCAGGAAAAATAAGTGAAAATCAGGTGAATAGAACGCACCTTTGGTTCATTGCAGCTTACTGTAGTTTATTTGCATTCGTGACATATCTCTAACTTACACGGGATATACATGTGCAGTTGTTGGAAAGCTTTCAAGAATCATTCCCGACATTACAATTTCCTCCTCTCCCTGAACGTGGAGATTTTGACTTACGAGAAGTTCTGCAGTCTACCTATTTCTTCAATTAGTTTGACTGCATTATTAGTATATAGCCTTCACTTAATCATACACAAAAGAAGAAGATGATCCAATAAGATAATTTCTCGGTATCCATGGTTCATTAGCATGTGAGAAAAGCATTGGATTCCTACCCCTCCCGAAAGTGGAGATGGCAAACAGGAAAAATTTCTGTGTGGACCATCTGCCTGCACATTTTATGATGATGCAAATACATAGCCATTTGCCTGCACATTGGATCAGAGTTCCCTCCGTGTTTTATTGGTTGGGATACCTGCCTTTATGAACTTGAACCCTCGAAGAAGAAGAGAAATAGAGAGAAATTTGTGAACTTGAACCCTCGAAGAAAAAGAAGAGAAATAGAGAGAAATTTATGAACTTGAATCCTCGAAGAATTGAAATAGAGAGAGAAGTTTGTGAAGGAAACAGGATCGAATCCTCTATGGCAGTTGCAGTAAGCAGATTCTACAGCAGCTGTACATGATGGTGATGCAGAAGCAGCAACAAGATGGTGAGGGGGCATTTTTGTAATTGATTAGTGATTACTTATTTTTCGGACAGTAAAAACAGAACAAAGTGCGAGCATATCCATTGATATGGAGTGTTATCTGCATAGATTCATATACGCGTTACTGTTATTAATGTATACGTGTGTTCTTTTCTCTTCTTTGAGCACTTATGAATTGTGTAGAATGAAGTTGTAGGAACACATTTTTTGACACAGAAGTGTCCTGTTATAAGAATGTGAAGTTTGTCTTGTCTGAACTTTCATTGATTTATAGCAAATCCTGTGTTATTACATTGTATAGTGCAAGGATGAATATGAACATTGTATGAAGTATACTGTAATAAAGAATGTGAAGGTTGTCTGAACTTTCATTGATTTGTTGCAAATCCTGTGTTATATTACTTTGTTTTGTGTAAGGATGAATTCATTGGACAATGTGCTTTGTACTTTCTCTGTTTTTCACTTGCAATTTGATACTCCCTCTG

mRNA sequence

ATGAACTTGAATCCTCGAAGAATTGAAATAGAGAGAGAAGTTTGTGAAGGAAACAGGATCGAATCCTCTATGGCAGTTGCAATCATGGTTAAGCGCCAGAGAACAGCATTTCCTCCAAATTTTGTCCACTCTCTTGATGGTTCCCACATGATGATGACTGCCGTTGCCTGCAAACAAGCTGGCATGTACTTTGCAGGTGTTCATGATTCATATTGGACACATGCATGTGATGTTGACGAAATGAACCGGATATTAAGGGTGAAGTTTGTTGAGCTCTATGAACAACCAATACTTGAGAATTTGTTGGAAAGCTTTCAAGAATCATTCCCGACATTACAATTTCCTCCTCTCCCTGAACGTGGAGATTTTGACTTACGAGAAGTTCTGCAGTCTACCTATTTCTTCAATTAGTTTGACTGCATTATTAGTATATAGCCTTCACTTAATCATACACAAAAGAAGAAGATGATCCAATAAGATAATTTCTCGGTATCCATGGTTCATTAGCATGTGAGAAAAGCATTGGATTCCTACCCCTCCCGAAAGTGGAGATGGCAAACAGGAAAAATTTCTGTGTGGACCATCTGCCTGCACATTTTATGATGATGCAAATACATAGCCATTTGCCTGCACATTGGATCAGAGTTCCCTCCGTGTTTTATTGGTTGGGATACCTGCCTTTATGAACTTGAACCCTCGAAGAAGAAGAGAAATAGAGAGAAATTTGTGAACTTGAACCCTCGAAGAAAAAGAAGAGAAATAGAGAGAAATTTATGAACTTGAATCCTCGAAGAATTGAAATAGAGAGAGAAGTTTGTGAAGGAAACAGGATCGAATCCTCTATGGCAGTTGCAGTAAGCAGATTCTACAGCAGCTGTACATGATGGTGATGCAGAAGCAGCAACAAGATGGTGAGGGGGCATTTTTGTAATTGATTAGTGATTACTTATTTTTCGGACAGTAAAAACAGAACAAAGTGCGAGCATATCCATTGATATGGAGTGTTATCTGCATAGATTCATATACGCGTTACTGTTATTAATGTATACGTGTGTTCTTTTCTCTTCTTTGAGCACTTATGAATTGTGTAGAATGAAGTTGTAGGAACACATTTTTTGACACAGAAGTGTCCTGTTATAAGAATGTGAAGTTTGTCTTGTCTGAACTTTCATTGATTTATAGCAAATCCTGTGTTATTACATTGTATAGTGCAAGGATGAATATGAACATTGTATGAAGTATACTGTAATAAAGAATGTGAAGGTTGTCTGAACTTTCATTGATTTGTTGCAAATCCTGTGTTATATTACTTTGTTTTGTGTAAGGATGAATTCATTGGACAATGTGCTTTGTACTTTCTCTGTTTTTCACTTGCAATTTGATACTCCCTCTG

Coding sequence (CDS)

ATGAACTTGAATCCTCGAAGAATTGAAATAGAGAGAGAAGTTTGTGAAGGAAACAGGATCGAATCCTCTATGGCAGTTGCAATCATGGTTAAGCGCCAGAGAACAGCATTTCCTCCAAATTTTGTCCACTCTCTTGATGGTTCCCACATGATGATGACTGCCGTTGCCTGCAAACAAGCTGGCATGTACTTTGCAGGTGTTCATGATTCATATTGGACACATGCATGTGATGTTGACGAAATGAACCGGATATTAAGGGTGAAGTTTGTTGAGCTCTATGAACAACCAATACTTGAGAATTTGTTGGAAAGCTTTCAAGAATCATTCCCGACATTACAATTTCCTCCTCTCCCTGAACGTGGAGATTTTGACTTACGAGAAGTTCTGCAGTCTACCTATTTCTTCAATTAG

Protein sequence

MNLNPRRIEIEREVCEGNRIESSMAVAIMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRVKFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo02373	Spo02373	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo02373.1	Spo02373.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo02373.1.exon.1	Spo02373.1.exon.1	exon
Spo02373.1.exon.2	Spo02373.1.exon.2	exon
Spo02373.1.exon.3	Spo02373.1.exon.3	exon
Spo02373.1.exon.4	Spo02373.1.exon.4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo02373.1.CDS.1	Spo02373.1.CDS.1	CDS
Spo02373.1.CDS.2	Spo02373.1.CDS.2	CDS
Spo02373.1.CDS.3	Spo02373.1.CDS.3	CDS
Spo02373.1.CDS.4	Spo02373.1.CDS.4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo02373.1.utr3p.1	Spo02373.1.utr3p.1	three_prime_UTR

Homology

[Close]

BLAST of Spo02373.1 vs. NCBI nr
Match: gi|902231101|gb|KNA22118.1| (hypothetical protein SOVF_036990 [Spinacia oleracea])

HSP 1 Score: 229.6 bits (584), Expect = 3.400e-57
Identity = 108/109 (99.08%), Postives = 109/109 (100.00%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV
Sbjct: 878 IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 937

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLR+VLQSTYFFN
Sbjct: 938 KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLRDVLQSTYFFN 986

[Close]

BLAST of Spo02373.1 vs. NCBI nr
Match: gi|1865721|emb|CAA69305.1| (mitochondrial single-subunit DNA-dependent RNA polymerase [Chenopodium album])

HSP 1 Score: 214.5 bits (545), Expect = 1.100e-52
Identity = 99/109 (90.83%), Postives = 105/109 (96.33%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           +M KRQRTAFPPNFVHSLDGSHMMMTA+ACK+AGM FAGVHDSYWTHACDVD+MN+ILR 
Sbjct: 880 VMAKRQRTAFPPNFVHSLDGSHMMMTAIACKKAGMNFAGVHDSYWTHACDVDKMNQILRE 939

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVL+S YFFN
Sbjct: 940 KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLESPYFFN 988

[Close]

BLAST of Spo02373.1 vs. NCBI nr
Match: gi|743797885|ref|XP_011009271.1| (PREDICTED: LOW QUALITY PROTEIN: DNA-directed RNA polymerase 1B, mitochondrial [Populus euphratica])

HSP 1 Score: 213.4 bits (542), Expect = 2.500e-52
Identity = 99/109 (90.83%), Postives = 104/109 (95.41%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           +MVKRQRTAFPPNFVHSLDGSHMMMTAVACK+AG+ FAGVHDSYWTHACDVDEMNRILR 
Sbjct: 884 VMVKRQRTAFPPNFVHSLDGSHMMMTAVACKEAGLNFAGVHDSYWTHACDVDEMNRILRE 943

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYE PILENLLESFQ SFP L+FPPLPERGDFDLR+VLQSTYFFN
Sbjct: 944 KFVELYETPILENLLESFQSSFPNLKFPPLPERGDFDLRDVLQSTYFFN 992

[Close]

BLAST of Spo02373.1 vs. NCBI nr
Match: gi|566190843|ref|XP_002315996.2| (DNA-directed RNA polymerase family protein [Populus trichocarpa])

HSP 1 Score: 213.4 bits (542), Expect = 2.500e-52
Identity = 99/109 (90.83%), Postives = 104/109 (95.41%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           +MVKRQRTAFPPNFVHSLDGSHMMMTAVACK+AG+ FAGVHDSYWTHACDVDEMNRILR 
Sbjct: 754 VMVKRQRTAFPPNFVHSLDGSHMMMTAVACKEAGLNFAGVHDSYWTHACDVDEMNRILRE 813

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYE PILENLLESFQ SFP L+FPPLPERGDFDLR+VLQSTYFFN
Sbjct: 814 KFVELYETPILENLLESFQSSFPNLKFPPLPERGDFDLRDVLQSTYFFN 862

[Close]

BLAST of Spo02373.1 vs. NCBI nr
Match: gi|731340034|ref|XP_010681166.1| (PREDICTED: DNA-directed RNA polymerase 1B, mitochondrial-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 212.2 bits (539), Expect = 5.500e-52
Identity = 98/109 (89.91%), Postives = 105/109 (96.33%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           IMVKRQRTAFPPNFVHSLDGSHMMMTA+ACKQAG+ FAGVHDSYWTHAC+VDEMNRILR 
Sbjct: 878 IMVKRQRTAFPPNFVHSLDGSHMMMTAIACKQAGLNFAGVHDSYWTHACNVDEMNRILRE 937

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYEQPILENLL+SFQ+SFP L+FPPLPERGDFDLREVL+S YFFN
Sbjct: 938 KFVELYEQPILENLLKSFQDSFPALEFPPLPERGDFDLREVLESPYFFN 986

[Close]

BLAST of Spo02373.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RRN7_SPIOL (DNA-directed RNA polymerase OS=Spinacia oleracea GN=SOVF_036990 PE=3 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 2.300e-57
Identity = 108/109 (99.08%), Postives = 109/109 (100.00%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV
Sbjct: 878 IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 937

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLR+VLQSTYFFN
Sbjct: 938 KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLRDVLQSTYFFN 986

[Close]

BLAST of Spo02373.1 vs. UniProtKB/TrEMBL
Match: P93661_CHEAL (DNA-directed RNA polymerase OS=Chenopodium album PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 7.800e-53
Identity = 99/109 (90.83%), Postives = 105/109 (96.33%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           +M KRQRTAFPPNFVHSLDGSHMMMTA+ACK+AGM FAGVHDSYWTHACDVD+MN+ILR 
Sbjct: 880 VMAKRQRTAFPPNFVHSLDGSHMMMTAIACKKAGMNFAGVHDSYWTHACDVDKMNQILRE 939

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVL+S YFFN
Sbjct: 940 KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLESPYFFN 988

[Close]

BLAST of Spo02373.1 vs. UniProtKB/TrEMBL
Match: B9HWT3_POPTR (DNA-directed RNA polymerase OS=Populus trichocarpa GN=POPTR_0010s14650g PE=3 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 1.700e-52
Identity = 99/109 (90.83%), Postives = 104/109 (95.41%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           +MVKRQRTAFPPNFVHSLDGSHMMMTAVACK+AG+ FAGVHDSYWTHACDVDEMNRILR 
Sbjct: 754 VMVKRQRTAFPPNFVHSLDGSHMMMTAVACKEAGLNFAGVHDSYWTHACDVDEMNRILRE 813

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYE PILENLLESFQ SFP L+FPPLPERGDFDLR+VLQSTYFFN
Sbjct: 814 KFVELYETPILENLLESFQSSFPNLKFPPLPERGDFDLRDVLQSTYFFN 862

[Close]

BLAST of Spo02373.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C909_BETVU (DNA-directed RNA polymerase OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g140140 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 3.900e-52
Identity = 98/109 (89.91%), Postives = 105/109 (96.33%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           IMVKRQRTAFPPNFVHSLDGSHMMMTA+ACKQAG+ FAGVHDSYWTHAC+VDEMNRILR 
Sbjct: 808 IMVKRQRTAFPPNFVHSLDGSHMMMTAIACKQAGLNFAGVHDSYWTHACNVDEMNRILRE 867

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYEQPILENLL+SFQ+SFP L+FPPLPERGDFDLREVL+S YFFN
Sbjct: 868 KFVELYEQPILENLLKSFQDSFPALEFPPLPERGDFDLREVLESPYFFN 916

[Close]

BLAST of Spo02373.1 vs. UniProtKB/TrEMBL
Match: A5AW22_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_011556 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 5.000e-52
Identity = 98/117 (83.76%), Postives = 107/117 (91.45%), Query Frame = 1


		  
Query: 20  IESSMAVAIMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVD 79
           I + +   IMVKRQRTAFPPNFVHSLDGSHMMMTA+ACK+AG+ FAGVHDSYWTHACDVD
Sbjct: 52  ISTYLIFLIMVKRQRTAFPPNFVHSLDGSHMMMTAIACKKAGLNFAGVHDSYWTHACDVD 111

Query: 80  EMNRILRVKFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           EMNRILR KFV+LYE PILENLLESFQ+SFP L+FPPLPERGDFDLREVL+S YFFN
Sbjct: 112 EMNRILREKFVQLYETPILENLLESFQQSFPALEFPPLPERGDFDLREVLESPYFFN 168

[Close]

BLAST of Spo02373.1 vs. ExPASy Swiss-Prot
Match: RPO1B_TOBAC (DNA-directed RNA polymerase 1B, mitochondrial OS=Nicotiana tabacum GN=RPOT1-TOM PE=2 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 6.500e-53
Identity = 94/109 (86.24%), Postives = 104/109 (95.41%), Query Frame = 1


		  
Query: 28   IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
            +MVKRQRTAFPPNFVHSLDGSHMMMTA+ACK++G+ FAGVHDSYWTHACDVD+MN+ILR 
Sbjct: 894  VMVKRQRTAFPPNFVHSLDGSHMMMTAIACKESGLSFAGVHDSYWTHACDVDQMNKILRE 953

Query: 88   KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
            KFVELY+ PILENLLESFQ+SFP LQFPPLPERGDFDLREVL+S YFFN
Sbjct: 954  KFVELYDAPILENLLESFQQSFPDLQFPPLPERGDFDLREVLESPYFFN 1002

[Close]

BLAST of Spo02373.1 vs. ExPASy Swiss-Prot
Match: RPOT1_NICSY (DNA-directed RNA polymerase 1, mitochondrial OS=Nicotiana sylvestris GN=RPOT1 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 9.500e-52
Identity = 93/109 (85.32%), Postives = 103/109 (94.50%), Query Frame = 1


		  
Query: 28   IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
            +MVKRQRTAFPPNFVHSLDGSHMMMTA+ACK++G+ FAGVHDSYWTHA DVD+MN+ILR 
Sbjct: 894  VMVKRQRTAFPPNFVHSLDGSHMMMTAIACKESGLSFAGVHDSYWTHASDVDQMNKILRE 953

Query: 88   KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
            KFVELY+ PILENLLESFQ+SFP LQFPPLPERGDFDLREVL+S YFFN
Sbjct: 954  KFVELYDAPILENLLESFQQSFPDLQFPPLPERGDFDLREVLESPYFFN 1002

[Close]

BLAST of Spo02373.1 vs. ExPASy Swiss-Prot
Match: RPO1A_TOBAC (DNA-directed RNA polymerase 1A (Fragment) OS=Nicotiana tabacum GN=RPOT1-SYL PE=3 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 9.500e-52
Identity = 93/109 (85.32%), Postives = 103/109 (94.50%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           +MVKRQRTAFPPNFVHSLDGSHMMMTA+ACK++G+ FAGVHDSYWTHA DVD+MN+ILR 
Sbjct: 47  VMVKRQRTAFPPNFVHSLDGSHMMMTAIACKESGLSFAGVHDSYWTHASDVDQMNKILRE 106

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELY+ PILENLLESFQ+SFP LQFPPLPERGDFDLREVL+S YFFN
Sbjct: 107 KFVELYDAPILENLLESFQQSFPDLQFPPLPERGDFDLREVLESPYFFN 155

[Close]

BLAST of Spo02373.1 vs. ExPASy Swiss-Prot
Match: RPO2B_TOBAC (DNA-directed RNA polymerase 2B, chloroplastic/mitochondrial OS=Nicotiana tabacum GN=RPOT2-TOM PE=2 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 3.600e-51
Identity = 92/109 (84.40%), Postives = 102/109 (93.58%), Query Frame = 1


		  
Query: 28   IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
            +MVKRQRTAFPPNF+HSLDGSHMMMTAVAC++AG+ FAGVHDSYWTHACDVD++NRILR 
Sbjct: 913  VMVKRQRTAFPPNFIHSLDGSHMMMTAVACRRAGLNFAGVHDSYWTHACDVDKLNRILRE 972

Query: 88   KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
            KFVELYE PILE LLESFQ S+PTL FPPLPERGDFDLR+VL+S YFFN
Sbjct: 973  KFVELYETPILEKLLESFQTSYPTLLFPPLPERGDFDLRDVLESPYFFN 1021

[Close]

BLAST of Spo02373.1 vs. ExPASy Swiss-Prot
Match: RPOT2_NICSY (DNA-directed RNA polymerase 2, chloroplastic/mitochondrial OS=Nicotiana sylvestris GN=RPOT2 PE=2 SV=2)

HSP 1 Score: 201.4 bits (511), Expect = 6.100e-51
Identity = 91/109 (83.49%), Postives = 102/109 (93.58%), Query Frame = 1


		  
Query: 28   IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
            +MVKRQRTAFPPNF+HSLDGSHMMMTAVAC++AG+ FAGVHDSYWTHACDVD++NRILR 
Sbjct: 912  VMVKRQRTAFPPNFIHSLDGSHMMMTAVACRRAGLNFAGVHDSYWTHACDVDKLNRILRE 971

Query: 88   KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
            KFVELYE PILE LLESFQ S+PTL FPPLPERGDFD+R+VL+S YFFN
Sbjct: 972  KFVELYEAPILEKLLESFQTSYPTLLFPPLPERGDFDMRDVLESPYFFN 1020

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BLAST of Spo02373.1 vs. TAIR (Arabidopsis)
Match: AT1G68990.2 (male gametophyte defective 3)

HSP 1 Score: 194.1 bits (492), Expect = 5.500e-50
Identity = 88/109 (80.73%), Postives = 99/109 (90.83%), Query Frame = 1


		  
Query: 28  IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
           +M +RQ TAF PNF+HSLDGSHMMMTAVAC +AG+ FAGVHDS+WTHACDVD MN ILR 
Sbjct: 875 VMARRQMTAFAPNFIHSLDGSHMMMTAVACNRAGLSFAGVHDSFWTHACDVDVMNTILRE 934

Query: 88  KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFFN 137
           KFVELYE+PILENLLESFQ+SFP + FPPLPERGDFDLR+VL+STYFFN
Sbjct: 935 KFVELYEKPILENLLESFQKSFPDISFPPLPERGDFDLRKVLESTYFFN 983

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BLAST of Spo02373.1 vs. TAIR (Arabidopsis)
Match: AT2G24120.1 (DNA/RNA polymerases superfamily protein)

HSP 1 Score: 192.2 bits (487), Expect = 2.100e-49
Identity = 89/121 (73.55%), Postives = 104/121 (85.95%), Query Frame = 1


		  
Query: 16  EGNRIESSMAVAIMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHA 75
           EGN ++        V++QRTAFPPNFVHSLDG+HMMMTAVAC++AG+ FAGVHDSYWTHA
Sbjct: 881 EGNTVD--------VRKQRTAFPPNFVHSLDGTHMMMTAVACREAGLNFAGVHDSYWTHA 940

Query: 76  CDVDEMNRILRVKFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQSTYFF 135
           CDVD MNRILR KFVELY  PILE+LL+SFQES+P L FPP+P+RGDFDL+EVL+S YFF
Sbjct: 941 CDVDTMNRILREKFVELYNTPILEDLLQSFQESYPNLVFPPVPKRGDFDLKEVLKSQYFF 993

Query: 136 N 137
           N
Sbjct: 1001 N 993

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BLAST of Spo02373.1 vs. TAIR (Arabidopsis)
Match: AT5G15700.2 (DNA/RNA polymerases superfamily protein)

HSP 1 Score: 180.6 bits (457), Expect = 6.300e-46
Identity = 82/105 (78.10%), Postives = 95/105 (90.48%), Query Frame = 1


		  
Query: 28   IMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMYFAGVHDSYWTHACDVDEMNRILRV 87
            ++V+RQRTAFPPNF+HSLDGSHMMMTAVACK+AG+ FAGVHDS+WTHACDVD++N ILR 
Sbjct: 903  VIVRRQRTAFPPNFIHSLDGSHMMMTAVACKRAGVCFAGVHDSFWTHACDVDKLNIILRE 962

Query: 88   KFVELYEQPILENLLESFQESFPTLQFPPLPERGDFDLREVLQST 133
            KFVELY QPILENLLESF++SFP L FPPLPERGD DL+  + ST
Sbjct: 963  KFVELYSQPILENLLESFEQSFPHLDFPPLPERGDLDLKVNIVST 1007

The following BLAST results are available for this feature:

BLAST of Spo02373.1 vs. NCBI nr
BLAST of Spo02373.1 vs. UniProtKB/TrEMBL
BLAST of Spo02373.1 vs. ExPASy Swiss-Prot
BLAST of Spo02373.1 vs. TAIR (Arabidopsis)

BLAST of Spo02373.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902231101\|gb\|KNA22118.1\|	3.4e-57	99.0	hypothetical protein SOVF_0369...	[more]
gi\|1865721\|emb\|CAA69305.1\|	1.1e-52	90.8	mitochondrial single-subunit D...	[more]
gi\|743797885\|ref\|XP_011009271.1\|	2.5e-52	90.8	PREDICTED: LOW QUALITY PROTEIN...	[more]
gi\|566190843\|ref\|XP_002315996.2\|	2.5e-52	90.8	DNA-directed RNA polymerase fa...	[more]
gi\|731340034\|ref\|XP_010681166.1\|	5.5e-52	89.9	PREDICTED: DNA-directed RNA po...	[more]

BLAST of Spo02373.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RRN7_SPIOL	2.3e-57	99.0	DNA-directed RNA polymerase OS...	[more]
P93661_CHEAL	7.8e-53	90.8	DNA-directed RNA polymerase OS...	[more]
B9HWT3_POPTR	1.7e-52	90.8	DNA-directed RNA polymerase OS...	[more]
A0A0J8C909_BETVU	3.9e-52	89.9	DNA-directed RNA polymerase OS...	[more]
A5AW22_VITVI	5.0e-52	83.7	Putative uncharacterized prote...	[more]

BLAST of Spo02373.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
RPO1B_TOBAC	6.5e-53	86.2	DNA-directed RNA polymerase 1B...	[more]
RPOT1_NICSY	9.5e-52	85.3	DNA-directed RNA polymerase 1,...	[more]
RPO1A_TOBAC	9.5e-52	85.3	DNA-directed RNA polymerase 1A...	[more]
RPO2B_TOBAC	3.6e-51	84.4	DNA-directed RNA polymerase 2B...	[more]
RPOT2_NICSY	6.1e-51	83.4	DNA-directed RNA polymerase 2,...	[more]

BLAST of Spo02373.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G68990.2	5.5e-50	80.7	male gametophyte defective 3	[more]
AT2G24120.1	2.1e-49	73.5	DNA/RNA polymerases superfamil...	[more]
AT5G15700.2	6.3e-46	78.1	DNA/RNA polymerases superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002092	DNA-directed RNA polymerase, phage-type	PANTHER	PTHR10102	DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL	coord: 27..136 score: 2.4
IPR002092	DNA-directed RNA polymerase, phage-type	PFAM	PF00940	RNA_pol	coord: 22..136 score: 2.5
None	No IPR available	GENE3D	3.30.70.370		coord: 44..125 score: 6.3
None	No IPR available	PANTHER	PTHR10102:SF1	DNA-DIRECTED RNA POLYMERASE 3, CHLOROPLASTIC	coord: 27..136 score: 2.4
None	No IPR available	unknown	SSF56672	DNA/RNA polymerases	coord: 27..136 score: 3.65

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity