Spo01950.1 (mRNA) | SpinachBase

Overview


Name	Spo01950.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Lecithine-cholesterol acyltransferase-like 4
Location	SpoScf_24360 : 961 .. 1290 (-)
Sequence length	330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTGATGGGCTAAAAGCTGAAGCTAGGGTTGGAGTACCTGCTGAACACCGTGGCATCCTTTCTGACCATCATGTGTTGAGAATACTGAAGCATTGGTTGAATGCAGGTGAACCTGATCCCTTCTATGACCCTCTGAATGATTATGTGGTTCTGCCCACTGCTTTTGAAATTGAGAGGGTTAAAGAGAATGGCTTTCATGTAACTACGTTAAAGGAAGAATGGGAAATTATACCTACAGAATCGGATGATGTGGAAGGTTTGGAGCATCCATTAAGTCTTATATCCGTTTCTGAGGTAACAACTCATGTTCCACACGTCCCTGTGCAATGA

mRNA sequence

GCTGATGGGCTAAAAGCTGAAGCTAGGGTTGGAGTACCTGCTGAACACCGTGGCATCCTTTCTGACCATCATGTGTTGAGAATACTGAAGCATTGGTTGAATGCAGGTGAACCTGATCCCTTCTATGACCCTCTGAATGATTATGTGGTTCTGCCCACTGCTTTTGAAATTGAGAGGGTTAAAGAGAATGGCTTTCATGTAACTACGTTAAAGGAAGAATGGGAAATTATACCTACAGAATCGGATGATGTGGAAGGTTTGGAGCATCCATTAAGTCTTATATCCGTTTCTGAGGTAACAACTCATGTTCCACACGTCCCTGTGCAATGA

Coding sequence (CDS)

GCTGATGGGCTAAAAGCTGAAGCTAGGGTTGGAGTACCTGCTGAACACCGTGGCATCCTTTCTGACCATCATGTGTTGAGAATACTGAAGCATTGGTTGAATGCAGGTGAACCTGATCCCTTCTATGACCCTCTGAATGATTATGTGGTTCTGCCCACTGCTTTTGAAATTGAGAGGGTTAAAGAGAATGGCTTTCATGTAACTACGTTAAAGGAAGAATGGGAAATTATACCTACAGAATCGGATGATGTGGAAGGTTTGGAGCATCCATTAAGTCTTATATCCGTTTCTGAGGTAACAACTCATGTTCCACACGTCCCTGTGCAATGA

Protein sequence

ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERVKENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVTTHVPHVPVQ

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo01950	Spo01950	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo01950.1	Spo01950.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo01950.1.CDS.1	Spo01950.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo01950.1.exon.1	Spo01950.1.exon.1	exon

Homology

[Close]

BLAST of Spo01950.1 vs. NCBI nr
Match: gi|902237825|gb|KNA24954.1| (hypothetical protein SOVF_010990 [Spinacia oleracea])

HSP 1 Score: 229.2 bits (583), Expect = 3.500e-57
Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV
Sbjct: 399 ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 458

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVTTHVPHVPVQ 110
           KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVTTHVPHVPVQ
Sbjct: 459 KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVTTHVPHVPVQ 507

[Close]

BLAST of Spo01950.1 vs. NCBI nr
Match: gi|731351802|ref|XP_010687219.1| (PREDICTED: lecithin-cholesterol acyltransferase-like 4 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 166.8 bits (421), Expect = 2.100e-38
Identity = 83/113 (73.45%), Postives = 91/113 (80.53%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL AEAR+GVPAEHRGILSDHH  RILKHWLNAGEPDPFYDPLNDYVVLPTAFE+ERV
Sbjct: 399 ADGLNAEARIGVPAEHRGILSDHHAFRILKHWLNAGEPDPFYDPLNDYVVLPTAFELERV 458

Query: 61  KENGFHVTTLKEEWEIIPTE----SDDVEGLEHPLSLISVSEVTTHVPHVPVQ 110
           KE  F V   KEEWE+IP +     ++VE  EH +SLISVSEV +HV  V VQ
Sbjct: 459 KERCFQVA--KEEWEVIPKDLNVHDENVEVKEHQISLISVSEVASHVSRVSVQ 509

[Close]

BLAST of Spo01950.1 vs. NCBI nr
Match: gi|255566283|ref|XP_002524128.1| (PREDICTED: lecithin-cholesterol acyltransferase-like 4 [Ricinus communis])

HSP 1 Score: 143.3 bits (360), Expect = 2.500e-31
Identity = 63/100 (63.00%), Postives = 79/100 (79.00%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL AEARVG+P EHRGILSDHH+ RILKHWL AG+PDP+Y+P+NDYV+LPTAFEIE  
Sbjct: 399 ADGLSAEARVGIPGEHRGILSDHHLFRILKHWLKAGDPDPYYNPINDYVILPTAFEIETH 458

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVT 101
           KE G  VT+LKEEWEII  + D+++ +     ++S   V+
Sbjct: 459 KEKGVQVTSLKEEWEIISGDKDEIDNMADRSPVVSSISVS 498

[Close]

BLAST of Spo01950.1 vs. NCBI nr
Match: gi|641858680|gb|KDO77402.1| (hypothetical protein CISIN_1g009353mg [Citrus sinensis])

HSP 1 Score: 142.9 bits (359), Expect = 3.300e-31
Identity = 64/100 (64.00%), Postives = 77/100 (77.00%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL AEARVGVP EHRGI+ +HHV RILKHWL  G+PDPFY+P+NDYV+LPTA+E+ER 
Sbjct: 256 ADGLNAEARVGVPGEHRGIVCEHHVFRILKHWLKVGDPDPFYNPINDYVILPTAYEMERY 315

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVT 101
           KE G  VT+LKEEWEII  E DD + +     L+S   V+
Sbjct: 316 KEKGLQVTSLKEEWEIISEEQDDGDNMADRKPLVSSISVS 355

[Close]

BLAST of Spo01950.1 vs. NCBI nr
Match: gi|567912811|ref|XP_006448719.1| (hypothetical protein CICLE_v10014844mg [Citrus clementina])

HSP 1 Score: 142.9 bits (359), Expect = 3.300e-31
Identity = 64/100 (64.00%), Postives = 77/100 (77.00%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL AEARVGVP EHRGI+ +HHV RILKHWL  G+PDPFY+P+NDYV+LPTA+E+ER 
Sbjct: 399 ADGLNAEARVGVPGEHRGIVCEHHVFRILKHWLKVGDPDPFYNPINDYVILPTAYEMERY 458

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVT 101
           KE G  VT+LKEEWEII  E DD + +     L+S   V+
Sbjct: 459 KEKGLQVTSLKEEWEIISEEQDDGDNMADRKPLVSSISVS 498

[Close]

BLAST of Spo01950.1 vs. UniProtKB/TrEMBL
Match: A0A0K9S193_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_010990 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 2.400e-57
Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV
Sbjct: 399 ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 458

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVTTHVPHVPVQ 110
           KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVTTHVPHVPVQ
Sbjct: 459 KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVTTHVPHVPVQ 507

[Close]

BLAST of Spo01950.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BQE1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g188460 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 1.500e-38
Identity = 83/113 (73.45%), Postives = 91/113 (80.53%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL AEAR+GVPAEHRGILSDHH  RILKHWLNAGEPDPFYDPLNDYVVLPTAFE+ERV
Sbjct: 399 ADGLNAEARIGVPAEHRGILSDHHAFRILKHWLNAGEPDPFYDPLNDYVVLPTAFELERV 458

Query: 61  KENGFHVTTLKEEWEIIPTE----SDDVEGLEHPLSLISVSEVTTHVPHVPVQ 110
           KE  F V   KEEWE+IP +     ++VE  EH +SLISVSEV +HV  V VQ
Sbjct: 459 KERCFQVA--KEEWEVIPKDLNVHDENVEVKEHQISLISVSEVASHVSRVSVQ 509

[Close]

BLAST of Spo01950.1 vs. UniProtKB/TrEMBL
Match: B9SDQ9_RICCO (Phosphatidylcholine-sterol O-acyltransferase, putative OS=Ricinus communis GN=RCOM_1289750 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 1.800e-31
Identity = 63/100 (63.00%), Postives = 79/100 (79.00%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL AEARVG+P EHRGILSDHH+ RILKHWL AG+PDP+Y+P+NDYV+LPTAFEIE  
Sbjct: 399 ADGLSAEARVGIPGEHRGILSDHHLFRILKHWLKAGDPDPYYNPINDYVILPTAFEIETH 458

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVT 101
           KE G  VT+LKEEWEII  + D+++ +     ++S   V+
Sbjct: 459 KEKGVQVTSLKEEWEIISGDKDEIDNMADRSPVVSSISVS 498

[Close]

BLAST of Spo01950.1 vs. UniProtKB/TrEMBL
Match: V4W739_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014844mg PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.300e-31
Identity = 64/100 (64.00%), Postives = 77/100 (77.00%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL AEARVGVP EHRGI+ +HHV RILKHWL  G+PDPFY+P+NDYV+LPTA+E+ER 
Sbjct: 399 ADGLNAEARVGVPGEHRGIVCEHHVFRILKHWLKVGDPDPFYNPINDYVILPTAYEMERY 458

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVT 101
           KE G  VT+LKEEWEII  E DD + +     L+S   V+
Sbjct: 459 KEKGLQVTSLKEEWEIISEEQDDGDNMADRKPLVSSISVS 498

[Close]

BLAST of Spo01950.1 vs. UniProtKB/TrEMBL
Match: A0A067GPY8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009353mg PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.300e-31
Identity = 64/100 (64.00%), Postives = 77/100 (77.00%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL AEARVGVP EHRGI+ +HHV RILKHWL  G+PDPFY+P+NDYV+LPTA+E+ER 
Sbjct: 275 ADGLNAEARVGVPGEHRGIVCEHHVFRILKHWLKVGDPDPFYNPINDYVILPTAYEMERY 334

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSEVT 101
           KE G  VT+LKEEWEII  E DD + +     L+S   V+
Sbjct: 335 KEKGLQVTSLKEEWEIISEEQDDGDNMADRKPLVSSISVS 374

[Close]

BLAST of Spo01950.1 vs. ExPASy Swiss-Prot
Match: LCAT4_ARATH (Lecithin-cholesterol acyltransferase-like 4 OS=Arabidopsis thaliana GN=LCAT4 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 7.600e-28
Identity = 56/98 (57.14%), Postives = 75/98 (76.53%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL+A ARVGVP EHRGIL+DH V R+LK WLN GEPDPFY+P+NDYV+LPT +E E+ 
Sbjct: 401 ADGLEAVARVGVPGEHRGILNDHRVFRMLKKWLNVGEPDPFYNPVNDYVILPTTYEFEKF 460

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSE 99
            ENG  V ++KE W+II ++ +++      ++ ISVS+
Sbjct: 461 HENGLEVASVKESWDII-SDDNNIGTTGSTVNSISVSQ 497

[Close]

BLAST of Spo01950.1 vs. TAIR (Arabidopsis)
Match: AT4G19860.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 124.4 bits (311), Expect = 4.300e-29
Identity = 56/98 (57.14%), Postives = 75/98 (76.53%), Query Frame = 1


		  
Query: 1   ADGLKAEARVGVPAEHRGILSDHHVLRILKHWLNAGEPDPFYDPLNDYVVLPTAFEIERV 60
           ADGL+A ARVGVP EHRGIL+DH V R+LK WLN GEPDPFY+P+NDYV+LPT +E E+ 
Sbjct: 401 ADGLEAVARVGVPGEHRGILNDHRVFRMLKKWLNVGEPDPFYNPVNDYVILPTTYEFEKF 460

Query: 61  KENGFHVTTLKEEWEIIPTESDDVEGLEHPLSLISVSE 99
            ENG  V ++KE W+II ++ +++      ++ ISVS+
Sbjct: 461 HENGLEVASVKESWDII-SDDNNIGTTGSTVNSISVSQ 497

The following BLAST results are available for this feature:

BLAST of Spo01950.1 vs. NCBI nr
BLAST of Spo01950.1 vs. UniProtKB/TrEMBL
BLAST of Spo01950.1 vs. ExPASy Swiss-Prot
BLAST of Spo01950.1 vs. TAIR (Arabidopsis)

BLAST of Spo01950.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902237825\|gb\|KNA24954.1\|	3.5e-57	100.	hypothetical protein SOVF_0109...	[more]
gi\|731351802\|ref\|XP_010687219.1\|	2.1e-38	73.4	PREDICTED: lecithin-cholestero...	[more]
gi\|255566283\|ref\|XP_002524128.1\|	2.5e-31	63.0	PREDICTED: lecithin-cholestero...	[more]
gi\|641858680\|gb\|KDO77402.1\|	3.3e-31	64.0	hypothetical protein CISIN_1g0...	[more]
gi\|567912811\|ref\|XP_006448719.1\|	3.3e-31	64.0	hypothetical protein CICLE_v10...	[more]

BLAST of Spo01950.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9S193_SPIOL	2.4e-57	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8BQE1_BETVU	1.5e-38	73.4	Uncharacterized protein OS=Bet...	[more]
B9SDQ9_RICCO	1.8e-31	63.0	Phosphatidylcholine-sterol O-a...	[more]
V4W739_9ROSI	2.3e-31	64.0	Uncharacterized protein OS=Cit...	[more]
A0A067GPY8_CITSI	2.3e-31	64.0	Uncharacterized protein OS=Cit...	[more]

BLAST of Spo01950.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1


Match Name	E-value	Identity	Description
LCAT4_ARATH	7.6e-28	57.1	Lecithin-cholesterol acyltrans...	[more]

BLAST of Spo01950.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G19860.1	4.3e-29	57.1	alpha/beta-Hydrolases superfam...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11440	LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED	coord: 1..83 score: 3.5
None	No IPR available	PANTHER	PTHR11440:SF3	LECITHIN-CHOLESTEROL ACYLTRANSFERASE-LIKE 4	coord: 1..83 score: 3.5

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048193	Golgi vesicle transport
biological_process	GO:0042967	obsolete acyl-carrier-protein biosynthetic process
biological_process	GO:0009395	phospholipid catabolic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0008374	O-acyltransferase activity
molecular_function	GO:0004620	phospholipase activity