Spo22769 (gene)

Overview
NameSpo22769
Typegene
OrganismSpinacia oleracea (Spinach)
Description2-dehydro-3-deoxyphosphooctonate aldolase (KDO 8-P synthase)
LocationSpoScf_00655 : 28660 .. 30809 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACCAGAAGAAAGCAATAGGTGCCTCCACCCTCCTCTTCATCGCGGTTGCCCCTCCGCCATCTTCTCTCCTACCTTTGTTCTTCGATCTGCTGGTAAATGCGATCCAACTTCATAATCCGATCTGCAGCGCTCCATCGTCGTCTCTGGCAAGGTCTCCAAATAACACCTTCGAGGATTCCAATGCAAAGCACAGATTCAAAAAATCTATGGAATCCTCCAAGTTGTTATTGGAAATCTTGTTGACAGAGAGAGAGAGAAAAATTTGGAGATATCTCCAAAATTAAAGGTTTTTTTCTTTAATCCTCTTTTCTCTTTATTTCTGTAATTTTTTTTTGGTTTTGTACTATGTTTTGTTTTCAATTTTTCTGGGTGTACTTTATTGTTTTCTAATTCGTCGGAGCCATGGTCACCACCTGATTTGCCGCCCTCCGACATCCATAATGTGAGTTTGTTGTTTTTTTCTTTATTTTTGGAGGAAAATATTTGATTTTTAGCTCAAGTTTTATCAATTTTAGAATTTTTGATTATTTTAGTGAGAAATTAATATGGGTTTGTTCATGGTGGAAGTTTATAGTTTCCAAATTGGGTATTTTGATTTGCATTGAGGAATGAGGATCTTGGGATTGTTAATTCAAAGGTCTTAATTATTGTTTTAACTGATTTTATTTTCAGGTTTAGTATGTTGTTGATTGTTGTTTTGGAAGAATTTGAATGAAAAGTTGTACAATATTTTGTTTTCCAATTCAAATTGAAGCAAGTTCAACTAAGTGATTTTATCAGGTTCATATACTTTGCTCCCTTCAATCTTTAATTATGAGGTTGATAGTCAGGGCAGCTAATGACTTGGGGTTTTGTAGATACTTGTAATCATGTATTTATAGATGGGTTGAAGTTAATCACTTCTTAATCATGTTTTAGGATTTTGCTAATGACTGATTTTCTTCAGTTTTTGTCTTCAATTGCTGCTGACCTTGGTCTTTCTGCTTCGTGGGTGTAAATCAAGTTTTTTAACTGCTTTTTTGGGTTTTTTTTAGGGTGATAAAATTGTTTTTTGGGGTTAATATTACTGCTACTGAATTCAGTTCTGTAAATGGTCTATTACATTTTCTATTTCTGATTGATTTGAGATTTGACTTCTTGTGGTAAAAAAGTTGCTATTCTTGTTATCAGTTGTTGTCAAAATGGATTTGGTAAGACCTGTAATATGGAAATTTCACTCTTTTCAATATAGAATTTGGATTAACTTGTTCGTGAAGCTGTTAGATGAAGGTCATCCTGATGACTTGAGTCATTTTGATTACCTGGTGTGGCTTTTAGGTTCATTAAACACGACGTTGACCTTCTTTTCTGTGTTGTCTTTGCCTTTCGGTCTTCATTTCTGTGTCGTTGGAATACGTTGTCAACCGGTACTGTAACTTGTTGTAACTCATTGTAACTCAAATTCCTTACAGGCATTAAGGCAGCATAACCCTTTTATTGTTGATTGTCCTTTTCCCTTTACTTGTGCATATATCATATCTAATCACAATACTAATAAATATCAGCTATTGGTTTTCTTTTTCTTTACTACTAAAGGTAATTGTGAAAATGAGCAAAAGAATCCTCAGCTTGTTTGCTATCAGTGTCAGACCTTAGAAGCATAAAGTCATCTCTTTGCTTCTTTGCCTCTTAAGGCTTAATGGTGTGGGTGATCTTTAAAATCAAGATCACTAGAGATTACAGTGACATGGGGTTTCCAACTGTTATTATCTTGTATTGATTATAAGCTAATGTTGATCATGTTATATGCGACTTAAAATATGATAGATTGGTCATTGATAGATATATTCTTATCTTCAGATTTTGACCCTGTTGCATTGTTCCGTAAACATGATTTGTCTTAATATATTATCTTAACTTAAGAGAACTAATCTGAGTACTTTGACCTTATTGCATATGCAGTTTGACATCATCTCTAAGGAAATACGCTGAAAATATGGATTCTTCCGTATCTCTATATACCCAACTTAAGGCAACCCAATCTTTCTTCGTGACGGCGGGTCCAAATGTAATTGAGCCAGAAGAGCACGTTATGAAGATGGCTAAGCACATAAAAGGCTATAACAACCAAAGTTGGAGTGCCTCTGATCTTCAAGTCCAGCTTTGA

mRNA sequence

ATGAACCAGAAGAAAGCAATAGGTGCCTCCACCCTCCTCTTCATCGCGGTTGCCCCTCCGCCATCTTCTCTCCTACCTTTGTTCTTCGATCTGCTGGTAAATGCGATCCAACTTCATAATCCGATCTGCAGCGCTCCATCGTCGTCTCTGGCAAGGTCTCCAAATAACACCTTCGAGGATTCCAATGCAAAGCACAGATTCAAAAAATCTATGGAATCCTCCAAGTTTTTGACATCATCTCTAAGGAAATACGCTGAAAATATGGATTCTTCCGTATCTCTATATACCCAACTTAAGGCAACCCAATCTTTCTTCGTGACGGCGGGTCCAAATGTAATTGAGCCAGAAGAGCACGTTATGAAGATGGCTAAGCACATAAAAGGCTATAACAACCAAAGTTGGAGTGCCTCTGATCTTCAAGTCCAGCTTTGA

Coding sequence (CDS)

ATGAACCAGAAGAAAGCAATAGGTGCCTCCACCCTCCTCTTCATCGCGGTTGCCCCTCCGCCATCTTCTCTCCTACCTTTGTTCTTCGATCTGCTGGTAAATGCGATCCAACTTCATAATCCGATCTGCAGCGCTCCATCGTCGTCTCTGGCAAGGTCTCCAAATAACACCTTCGAGGATTCCAATGCAAAGCACAGATTCAAAAAATCTATGGAATCCTCCAAGTTTTTGACATCATCTCTAAGGAAATACGCTGAAAATATGGATTCTTCCGTATCTCTATATACCCAACTTAAGGCAACCCAATCTTTCTTCGTGACGGCGGGTCCAAATGTAATTGAGCCAGAAGAGCACGTTATGAAGATGGCTAAGCACATAAAAGGCTATAACAACCAAAGTTGGAGTGCCTCTGATCTTCAAGTCCAGCTTTGA

Protein sequence

MNQKKAIGASTLLFIAVAPPPSSLLPLFFDLLVNAIQLHNPICSAPSSSLARSPNNTFEDSNAKHRFKKSMESSKFLTSSLRKYAENMDSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIKGYNNQSWSASDLQVQL
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo22769.1Spo22769.1mRNA


Homology
[Close]
BLAST of Spo22769.1 vs. NCBI nr
Match: gi|902200827|gb|KNA13835.1| (hypothetical protein SOVF_112850 [Spinacia oleracea])

HSP 1 Score: 146.4 bits (368), Expect = 3.900e-32
Identity = 77/81 (95.06%), Postives = 77/81 (95.06%), Query Frame = 1 

		  

Query: 1  MNQKKAIGASTLLFIAVAPPPSSLLPLFFDLLVNAIQLHNPICSAPSSSLARSPNNTFED 60
          MNQKKAIGASTLLFIAVAPPPSSLLPLFFDLLVNAIQLHNPICSAPSSSLARSPNNTFED
Sbjct: 1  MNQKKAIGASTLLFIAVAPPPSSLLPLFFDLLVNAIQLHNPICSAPSSSLARSPNNTFED 60

Query: 61 SNAKHRFKKSMESSKFLTSSL 82
          SNAKHRFKKSMESSK L   L
Sbjct: 61 SNAKHRFKKSMESSKLLLEIL 81
[Close]
BLAST of Spo22769.1 vs. NCBI nr
Match: gi|902188798|gb|KNA11226.1| (hypothetical protein SOVF_137000 [Spinacia oleracea])

HSP 1 Score: 72.8 bits (177), Expect = 5.500e-10
Identity = 36/40 (90.00%), Postives = 36/40 (90.00%), Query Frame = 1 

		  

Query: 88  MDSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIK 128
           MDSSVSLYTQLKA Q FFV AGPNVIE EEHVMKMAKHIK
Sbjct: 1   MDSSVSLYTQLKAAQPFFVMAGPNVIESEEHVMKMAKHIK 40
[Close]
BLAST of Spo22769.1 vs. NCBI nr
Match: gi|902231427|gb|KNA22260.1| (hypothetical protein SOVF_035710 [Spinacia oleracea])

HSP 1 Score: 71.6 bits (174), Expect = 1.200e-9
Identity = 35/40 (87.50%), Postives = 36/40 (90.00%), Query Frame = 1 

		  

Query: 88  MDSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIK 128
           MDSSV+LYTQLKA Q FFV AGPNVIE EEHVMKMAKHIK
Sbjct: 1   MDSSVALYTQLKAAQPFFVMAGPNVIESEEHVMKMAKHIK 40
[Close]
BLAST of Spo22769.1 vs. NCBI nr
Match: gi|731317251|ref|XP_010668308.1| (PREDICTED: 2-dehydro-3-deoxyphosphooctonate aldolase 1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 66.2 bits (160), Expect = 5.200e-8
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 1 

		  

Query: 88  MDSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIK 128
           M+SS +LYTQLK  Q FFV AGPNVIE EEH+MKMAKHIK
Sbjct: 1   MESSTALYTQLKEAQPFFVMAGPNVIESEEHIMKMAKHIK 40
[Close]
BLAST of Spo22769.1 vs. NCBI nr
Match: gi|218186590|gb|EEC69017.1| (hypothetical protein OsI_37812 [Oryza sativa Indica Group])

HSP 1 Score: 65.9 bits (159), Expect = 6.700e-8
Identity = 34/45 (75.56%), Postives = 38/45 (84.44%), Query Frame = 1 

		  

Query: 85  AENMD-SSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIKG 129
           A+ MD SSV+LY QLKA Q FF+ AGPNVIE EEHV+KMAKHIKG
Sbjct: 170 ADAMDASSVALYGQLKAAQPFFLLAGPNVIESEEHVLKMAKHIKG 214
[Close]
BLAST of Spo22769.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R2U9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_112850 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 2.700e-32
Identity = 77/81 (95.06%), Postives = 77/81 (95.06%), Query Frame = 1 

		  

Query: 1  MNQKKAIGASTLLFIAVAPPPSSLLPLFFDLLVNAIQLHNPICSAPSSSLARSPNNTFED 60
          MNQKKAIGASTLLFIAVAPPPSSLLPLFFDLLVNAIQLHNPICSAPSSSLARSPNNTFED
Sbjct: 1  MNQKKAIGASTLLFIAVAPPPSSLLPLFFDLLVNAIQLHNPICSAPSSSLARSPNNTFED 60

Query: 61 SNAKHRFKKSMESSKFLTSSL 82
          SNAKHRFKKSMESSK L   L
Sbjct: 61 SNAKHRFKKSMESSKLLLEIL 81
[Close]
BLAST of Spo22769.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QXI8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_137000 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 3.800e-10
Identity = 36/40 (90.00%), Postives = 36/40 (90.00%), Query Frame = 1 

		  

Query: 88  MDSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIK 128
           MDSSVSLYTQLKA Q FFV AGPNVIE EEHVMKMAKHIK
Sbjct: 1   MDSSVSLYTQLKAAQPFFVMAGPNVIESEEHVMKMAKHIK 40
[Close]
BLAST of Spo22769.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RRT8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_035710 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 8.600e-10
Identity = 35/40 (87.50%), Postives = 36/40 (90.00%), Query Frame = 1 

		  

Query: 88  MDSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIK 128
           MDSSV+LYTQLKA Q FFV AGPNVIE EEHVMKMAKHIK
Sbjct: 1   MDSSVALYTQLKAAQPFFVMAGPNVIESEEHVMKMAKHIK 40
[Close]
BLAST of Spo22769.1 vs. UniProtKB/TrEMBL
Match: A0A0D3HSJ1_9ORYZ (Uncharacterized protein OS=Oryza barthii PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.100e-8
Identity = 35/45 (77.78%), Postives = 38/45 (84.44%), Query Frame = 1 

		  

Query: 85  AENMD-SSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIKG 129
           AE MD SSV+LY QLKA Q FF+ AGPNVIE EEHV+KMAKHIKG
Sbjct: 90  AEAMDASSVALYGQLKAAQPFFLLAGPNVIESEEHVLKMAKHIKG 134
[Close]
BLAST of Spo22769.1 vs. UniProtKB/TrEMBL
Match: A0A0D3HSJ2_9ORYZ (Uncharacterized protein OS=Oryza barthii PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.100e-8
Identity = 35/45 (77.78%), Postives = 38/45 (84.44%), Query Frame = 1 

		  

Query: 85  AENMD-SSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIKG 129
           AE MD SSV+LY QLKA Q FF+ AGPNVIE EEHV+KMAKHIKG
Sbjct: 73  AEAMDASSVALYGQLKAAQPFFLLAGPNVIESEEHVLKMAKHIKG 117
[Close]
BLAST of Spo22769.1 vs. ExPASy Swiss-Prot
Match: KDSA_PEA (2-dehydro-3-deoxyphosphooctonate aldolase OS=Pisum sativum GN=KDSA PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.100e-9
Identity = 29/40 (72.50%), Postives = 32/40 (80.00%), Query Frame = 1 

		  

Query: 88  MDSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIK 128
           MD SV LY QLKA   FF+ AGPNVIE EEH+M+MAKHIK
Sbjct: 1   MDPSVLLYNQLKAADPFFLLAGPNVIESEEHIMRMAKHIK 40
[Close]
BLAST of Spo22769.1 vs. ExPASy Swiss-Prot
Match: KDSA1_ARATH (2-dehydro-3-deoxyphosphooctonate aldolase 1 OS=Arabidopsis thaliana GN=KDSA1 PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 4.400e-7
Identity = 25/40 (62.50%), Postives = 32/40 (80.00%), Query Frame = 1 

		  

Query: 88  MDSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIK 128
           M ++  LY QLKA + FF+ AGPNVIE EEH+++MAKHIK
Sbjct: 1   MAATSLLYNQLKAAEPFFLLAGPNVIESEEHILRMAKHIK 40
[Close]
BLAST of Spo22769.1 vs. TAIR (Arabidopsis)
Match: AT1G16340.4 (Aldolase superfamily protein)

HSP 1 Score: 51.2 bits (121), Expect = 6.100e-7
Identity = 24/39 (61.54%), Postives = 30/39 (76.92%), Query Frame = 1 

		  

Query: 89  DSSVSLYTQLKATQSFFVTAGPNVIEPEEHVMKMAKHIK 128
           +S+  LY QLK  + FF+ AGPNVIE EEHV++MAK IK
Sbjct: 3   NSASLLYDQLKVAEPFFLLAGPNVIESEEHVLRMAKSIK 41
The following BLAST results are available for this feature:
BLAST of Spo22769.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902200827|gb|KNA13835.1|3.9e-3295.0hypothetical protein SOVF_1128... [more]
gi|902188798|gb|KNA11226.1|5.5e-1090.0hypothetical protein SOVF_1370... [more]
gi|902231427|gb|KNA22260.1|1.2e-987.5hypothetical protein SOVF_0357... [more]
gi|731317251|ref|XP_010668308.1|5.2e-877.5PREDICTED: 2-dehydro-3-deoxyph... [more]
gi|218186590|gb|EEC69017.1|6.7e-875.5hypothetical protein OsI_37812... [more]
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BLAST of Spo22769.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9R2U9_SPIOL2.7e-3295.0Uncharacterized protein OS=Spi... [more]
A0A0K9QXI8_SPIOL3.8e-1090.0Uncharacterized protein OS=Spi... [more]
A0A0K9RRT8_SPIOL8.6e-1087.5Uncharacterized protein OS=Spi... [more]
A0A0D3HSJ1_9ORYZ2.1e-877.7Uncharacterized protein OS=Ory... [more]
A0A0D3HSJ2_9ORYZ2.1e-877.7Uncharacterized protein OS=Ory... [more]
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BLAST of Spo22769.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2
Match NameE-valueIdentityDescription
KDSA_PEA6.1e-972.52-dehydro-3-deoxyphosphooctona... [more]
KDSA1_ARATH4.4e-762.52-dehydro-3-deoxyphosphooctona... [more]
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BLAST of Spo22769.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT1G16340.46.1e-761.5Aldolase superfamily protein[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70coord: 102..132
score: 9.

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018108 peptidyl-tyrosine phosphorylation
biological_process GO:0009103 lipopolysaccharide biosynthetic process
biological_process GO:0009966 regulation of signal transduction
biological_process GO:0009069 serine family amino acid metabolic process
cellular_component GO:0009536 plastid
cellular_component GO:0000785 chromatin
molecular_function GO:0008676 3-deoxy-8-phosphooctulonate synthase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0003682 chromatin binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004715 non-membrane spanning protein tyrosine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
RNA-Seq Expression
   



Co-expression
Gener valueExpression
Spo141290.70Barchart | Table
Spo087250.68Barchart | Table
Spo161860.66Barchart | Table
Spo069190.65Barchart | Table
Spo121300.65Barchart | Table