Spo22007 (gene) | SpinachBase

Overview


Name	Spo22007
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	(Sirohydrochlorin ferrochelatase, putative) (4.99.1.3)
Location	chr1 : 12217334 .. 12220709 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCCTGGTGTTACGAAGGTTTCAGATAAAACTTCCCATCCATGTAACCAAAGTGCGGAGTTTTTGTTGGTTGTTAAAGTAATTATATTAAGTTGACCCCGATCTCATTTGGTTTGCAGCTAAATAGTGGTGGGTTTTTTCTTGTTGGGATTTTTGGGTTAATATTATTGCTCCTGTTTAATCTTCCCAGCTGATGAATAGATGATAAGCACGATTAACAAGAACTCAAATGTCACTAAAGTTCTTGTCTTGTAATCAGTGGGTTCACCAGCTTCACCTTGAAAGGTATTTGATATTGAGACTCTTTTACCTAGGCATTCTCCCTTTACTCTAAAATTATTTTCACGCTCTTATTGCCCTTTTCCTTCTATTCTATGGTGGTCAGCCCGGTGTCATTAACTACCGCCAAAGGAAGTCGGATAGAAGTTGGACCTAGATTTCTTAGACTACCACAAAACACTCAACATAAGAGCCTGTCTACAAGTTTATGCTTTGCTATGGATCAGAAAATCGTGCATGAGGGTGCCTTTGGTGTTAGTCAGAAAGATGGTGTGATTATCGTTGATCATGGATCTCGTCGGAAAGAGTCCAATCTCTTACTGAGTAATGCTATTTAGAGTTGTTCATTTTCTTTGTCAATGCCTTTCCTGGGTAGACCTAGGAGCTATTTATGCAACTCTCTGTATTCTCTATTTCATTATGCAACGGTGTGCTTACTTGTTGTGTACTGCAGATGAATTTGTGCTGATGTTCAAAAGCAAAACTGGATACCCTATTGTTGAGCCGGCTCATATGGTACTCCCTACTTTTAGATATCATTATGCAATGTTTTCGAAAAAAAATTTAGGTGCACCCTGGTACACCGTACACCCAAGGGTACTTTTATGCCCATTGGTCGTTCCTCTAACATTTTCTCCCGACACGTAAGGGGAAAATGTGAGAGGGTGCACCTCAGGTGTGCTGATGTTTTCGTGATGGAGATGAGATTTCTGATTGAATCTGGAGCCAATCTCCATCGCCTTTCTGTTGTGTTGTGCAATTGGTGCACTTGTACAAACTAGAAAGATGCCAATTAAGACAATGATGGATCTGAAGTTTGGAACAGATAGTCATCATTTAAAACTGTCATTATGCAATGAATTTTTCTGTTCAATGTTTAGGAATTGGCAGAACCATCCATAAAAGATGCTTTCAATTCATGTGTCGAGCAAGGGGCAAATCGTGTTATCATAAGCCCATTCTTCCTATTTCCTGGAAGGCATTGGCATCAGGTAAGAAAACTAAATCACTCTGCTGCTTCTGCACTTTGATCACTATTTGCGAACTTATCGGTCATGGGGTACCCCCCCAAACACACACACACACACTCCTCTTCTTCCTAAAAACAAAGGAAGCCGCTGAAGCGTAAAAATAAAGTGACAGGAGTTTTTTGCATGTTGCAGTTGTTATGCCCTTTGTGTGCCTTTAATTGCAGGCTACTACATTACATTAGAATTTCATACAAGAATGTAACTTATCCTATCTCATGAAAGGGAAAGTAAATGATCCAGGGAAGGATGTTGATTCACTTTTAGGAAGGTTAAAGAGTCTGATGGAACCTTTCGATTTCTGAACATAAAATTTGAAACTGGCAATTTAAACAGGATTGCTATTGTTGTTGTACAGGATATTCCTTCCCTTGCAGATGCTGCTGCTCTGGAGCATCCCGGTATCAATTATCTTGTCACTGCACCGTTGGGTCTGCATGAGTTACTTGTGGTATTAACTGCATCCCTTCTAACTAGTATGATTTTATCTTTACTGCCCCTGTCTGCGTTTGGAAATTTTCAAGTGGTGAAAAGACATGGTGGTTATAAATGTTGTCTATTCTATTTTGTGTTTCATCTACGCATTACACATATTCACCCATCCAACCTCACCCGATTCAGATAGTGAACTGAAGTGATTTGTTACTTTCTTGATACCACCTGTGGCAAATATACGAAGTATAATTTATTGAACTTCAACTCTTCCCAGTGTTGTACAACTGCTGAATTCACTTGTTAGCTACTATTATCTTGAGATGATTGGATGATCATCACGCTGAAATAAGTTTTCTAAGTGACATTTAAGGCAGAATATATTGATTTTAATCTTGTGTGTTACGGTATCTGATCTTGCAGGATGTTGTGAAGGACAGAATTGACTATTGCTTAAGTCATGTAGCTGGAAAAGTGGAGGAATGTGCAGTCTGTGCTGGAACAGGCAGGTGTAAATTTAAAGTGCAGGATTCCGAAAATTAATGTGTAGTGATTTAAGCTACGCCGCACTTGTCAGTTTCATGGATCAAGCTAGATAGCATGTCTTTAGAACGTCAACTGTAATAACCGGAAGGTATGGCATTGCTATTTGGAGTACACATCACTTCCTGAGAAATATCTGCAGCATATGCCGTGGGTGCTGAATATCTGGATGTAGTGGCTTGTTTTCTCAGTGTATATGACAATCAATCTGAGTGGATCACGTGATAAATAAGTATTGTAACATCATCATGAATTCATGACAATGGTAATCTATGTACTGCATAAGACGGTCAGTGAAGAATTTTGCCATTTTTTTGAAAGGGTGACGAATGTAGCAGTTGTAGCATTTTTTTTATTTTTTTTTACATAAACTTAATTTGCATAAATAAAGAAATCGTACATATTACAACTTATTACTACTAATTTACTAAAACACCTTAATCTATTAGGGCCATTATAACCCCTTACATAGATTACACACCGGTAGCATTTCATATGTTCAACAAAACTTCATCACTCACTTCAATTCTTCAACATACTCGTCAAAAATTAGTTTGCCATTGATTTGATCATCCTTTGTTTAAAAAAAATTCATTTTGACTGTCACAATTAGAAAATTAGAAGAAGAAAAAAATGTTGACATTTGGAAAATGTATATTGAGACTTAAATAACATTTGACATTAAAACTTTTGATTTTAATATGGTTAAAGTGATATAAGAAATGCAAAAGTGAAAGTCATGTTGTTACTTTGAAATAAAGTTGTAAACTCTAGTGGTAACATTGCATATCAGGGGGGACTTAGATAGGTTGAGCAATGCAGAAGAGGAGGAAGGTCAGTCTTCCAGAAGTAAGAGTGCAGGTCAGCATTACAGTCATGTGGATTCGAGCATGGAAGTCAGAAGTACAACGCAGATCTTGTTTGGCACGATTCCAACTACAGTCATTACCACTACGACGACTGCAGGCAGGAGAAGCATGGGATTTGATGGTGGCAAGTCGGTTGCTGATGGTAATTTGGACACTTAGTTCTCGCTGGTGGCGTATCACTCTAGTCTGGAGTCAAAGCACAATCCTTAGAGGCGTGGCTAG

mRNA sequence

ATGGGCCCTGGTGTTACGAAGGTTTCAGATAAAACTTCCCATCCATCACGATTAACAAGAACTCAAATGTCACTAAAGTTCTTGTCTTGTAATCAGTGGGTTCACCAGCTTCACCTTGAAAGCCCGGTGTCATTAACTACCGCCAAAGGAAGTCGGATAGAAGTTGGACCTAGATTTCTTAGACTACCACAAAACACTCAACATAAGAGCCTGTCTACAAGTTTATGCTTTGCTATGGATCAGAAAATCGTGCATGAGGGTGCCTTTGGTGTTAGTCAGAAAGATGGTGTGATTATCGTTGATCATGGATCTCGTCGGAAAGAGTCCAATCTCTTACTGAATGAATTTGTGCTGATGTTCAAAAGCAAAACTGGATACCCTATTGTTGAGCCGGCTCATATGGAATTGGCAGAACCATCCATAAAAGATGCTTTCAATTCATGTGTCGAGCAAGGGGCAAATCGTGTTATCATAAGCCCATTCTTCCTATTTCCTGGAAGGCATTGGCATCAGGATATTCCTTCCCTTGCAGATGCTGCTGCTCTGGAGCATCCCGGTATCAATTATCTTGTCACTGCACCGTTGGGTCTGCATGAGTTACTTGTGGATGTTGTGAAGGACAGAATTGACTATTGCTTAAGTCATGTAGCTGGAAAAGTGGAGGAATGTGCAGTCTGTGCTGGAACAGGCAGGTGTAAATTTAAAGTGCAGGATTCCGAAAATTAATGTGTAGTGATTTAAGCTACGCCGCACTTGTCAGTTTCATGGATCAAGCTAGATAGCATGTCTTTAGAACGTCAACTGTAATAACCGGAAGGTATGGCATTGCTATTTGGAGTACACATCACTTCCTGAGAAATATCTGCAGCATATGCCGTGGGTGCTGAATATCTGGATGTAGTGGCTTGTTTTCTCAGTGTATATGACAATCAATCTGAGTGGATCACGTGATAAATAAGTATTGTAACATCATCATGAATTCATGACAATGGTAATCTATGTACTGCATAAGACGGGGGGACTTAGATAGGTTGAGCAATGCAGAAGAGGAGGAAGGTCAGTCTTCCAGAAGTAAGAGTGCAGGTCAGCATTACAGTCATGTGGATTCGAGCATGGAAGTCAGAAGTACAACGCAGATCTTGTTTGGCACGATTCCAACTACAGTCATTACCACTACGACGACTGCAGGCAGGAGAAGCATGGGATTTGATGGTGGCAAGTCGGTTGCTGATGGTAATTTGGACACTTAGTTCTCGCTGGTGGCGTATCACTCTAGTCTGGAGTCAAAGCACAATCCTTAGAGGCGTGGCTAG

Coding sequence (CDS)

ATGGGCCCTGGTGTTACGAAGGTTTCAGATAAAACTTCCCATCCATCACGATTAACAAGAACTCAAATGTCACTAAAGTTCTTGTCTTGTAATCAGTGGGTTCACCAGCTTCACCTTGAAAGCCCGGTGTCATTAACTACCGCCAAAGGAAGTCGGATAGAAGTTGGACCTAGATTTCTTAGACTACCACAAAACACTCAACATAAGAGCCTGTCTACAAGTTTATGCTTTGCTATGGATCAGAAAATCGTGCATGAGGGTGCCTTTGGTGTTAGTCAGAAAGATGGTGTGATTATCGTTGATCATGGATCTCGTCGGAAAGAGTCCAATCTCTTACTGAATGAATTTGTGCTGATGTTCAAAAGCAAAACTGGATACCCTATTGTTGAGCCGGCTCATATGGAATTGGCAGAACCATCCATAAAAGATGCTTTCAATTCATGTGTCGAGCAAGGGGCAAATCGTGTTATCATAAGCCCATTCTTCCTATTTCCTGGAAGGCATTGGCATCAGGATATTCCTTCCCTTGCAGATGCTGCTGCTCTGGAGCATCCCGGTATCAATTATCTTGTCACTGCACCGTTGGGTCTGCATGAGTTACTTGTGGATGTTGTGAAGGACAGAATTGACTATTGCTTAAGTCATGTAGCTGGAAAAGTGGAGGAATGTGCAGTCTGTGCTGGAACAGGCAGGTGTAAATTTAAAGTGCAGGATTCCGAAAATTAA

Protein sequence

MGPGVTKVSDKTSHPSRLTRTQMSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo22007.1	Spo22007.1	mRNA

Homology

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BLAST of Spo22007.1 vs. NCBI nr
Match: gi|902236265|gb|KNA24303.1| (hypothetical protein SOVF_017090 [Spinacia oleracea])

HSP 1 Score: 454.5 bits (1168), Expect = 1.100e-124
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 1


		  
Query: 23  MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 82
           MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK
Sbjct: 1   MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 60

Query: 83  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 142
           IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK
Sbjct: 61  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 120

Query: 143 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 202
           DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV
Sbjct: 121 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 180

Query: 203 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 242
           DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN
Sbjct: 181 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 219

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BLAST of Spo22007.1 vs. NCBI nr
Match: gi|902207097|gb|KNA15620.1| (hypothetical protein SOVF_096610 [Spinacia oleracea])

HSP 1 Score: 408.3 bits (1048), Expect = 9.300e-111
Identity = 195/219 (89.04%), Postives = 203/219 (92.69%), Query Frame = 1


		  
Query: 23  MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 82
           MSLKFLSCNQWVHQLHL+SPVS TT K S IE GPR+ +LPQ+T  KSLS SLCFAMDQK
Sbjct: 1   MSLKFLSCNQWVHQLHLQSPVSSTTTKRSWIEAGPRYFKLPQSTLRKSLSPSLCFAMDQK 60

Query: 83  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 142
            +HE AFGV QKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELA+PSIK
Sbjct: 61  SLHEDAFGVGQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELADPSIK 120

Query: 143 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 202
           DAFNSCV+QGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHE LV
Sbjct: 121 DAFNSCVQQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHEFLV 180

Query: 203 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 242
           DVVKDRI YCLSHVAG+VEECAVC GTGRCK KVQDSEN
Sbjct: 181 DVVKDRIGYCLSHVAGEVEECAVCVGTGRCKLKVQDSEN 219

[Close]

BLAST of Spo22007.1 vs. NCBI nr
Match: gi|731320521|ref|XP_010670852.1| (PREDICTED: uncharacterized protein LOC104887804 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 379.4 bits (973), Expect = 4.600e-102
Identity = 187/219 (85.39%), Postives = 197/219 (89.95%), Query Frame = 1


		  
Query: 23  MSLKFLSCNQWVHQLHLESPVS-LTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQ 82
           MSLK LSCNQWVH   L+SPVS L TAKGSR EV PR+   P++ Q K LS SLCF MDQ
Sbjct: 1   MSLKLLSCNQWVH---LQSPVSFLPTAKGSRTEVAPRYFNPPKSIQLKRLSPSLCFPMDQ 60

Query: 83  KIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSI 142
           K VHEGAFGV QKDGVIIVDHGSRRKESN+LLNEFVLMFKS+TGYPIVEPAHMELAEPSI
Sbjct: 61  KSVHEGAFGVGQKDGVIIVDHGSRRKESNILLNEFVLMFKSRTGYPIVEPAHMELAEPSI 120

Query: 143 KDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELL 202
           KDAF+ CV+QGAN VIISPFFLFPGRHWHQDIP LA AAALEHPGINYLVTAPLGLHELL
Sbjct: 121 KDAFDLCVQQGANCVIISPFFLFPGRHWHQDIPVLAGAAALEHPGINYLVTAPLGLHELL 180

Query: 203 VDVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSE 241
           VDVVKDRIDYCLSHVAG+VEECAVCAGTGRCKFKVQ+SE
Sbjct: 181 VDVVKDRIDYCLSHVAGQVEECAVCAGTGRCKFKVQNSE 216

[Close]

BLAST of Spo22007.1 vs. NCBI nr
Match: gi|1021044711|gb|KZN02492.1| (hypothetical protein DCAR_011246 [Daucus carota subsp. sativus])

HSP 1 Score: 273.5 bits (698), Expect = 3.600e-70
Identity = 125/177 (70.62%), Postives = 149/177 (84.18%), Query Frame = 1


		  
Query: 57  PRFLRLPQNTQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEF 116
           PRF++L  N++ +++ T LCFA       +   G+  KDGVIIVDHGSRRKESNL+LNEF
Sbjct: 30  PRFVKLSNNSK-RNVPTRLCFASSNGGFRQETSGIGSKDGVIIVDHGSRRKESNLMLNEF 89

Query: 117 VLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSL 176
           V+MF+ +TGYPIVEPAHMELAEPSIKDAF SCV+QG NRVI+SPFFLFPGRHW+QDIPSL
Sbjct: 90  VIMFRDRTGYPIVEPAHMELAEPSIKDAFASCVQQGVNRVIVSPFFLFPGRHWNQDIPSL 149

Query: 177 ADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
              AA EHPG++Y++TAPLGLHELLVDVV DRI +CLSHVAG  +EC+VCAGTGRC+
Sbjct: 150 TAEAAKEHPGVSYIITAPLGLHELLVDVVNDRIQHCLSHVAGDADECSVCAGTGRCQ 205

[Close]

BLAST of Spo22007.1 vs. NCBI nr
Match: gi|802542391|ref|XP_012081294.1| (PREDICTED: uncharacterized protein LOC105641377 [Jatropha curcas])

HSP 1 Score: 269.6 bits (688), Expect = 5.200e-69
Identity = 136/228 (59.65%), Postives = 162/228 (71.05%), Query Frame = 1


		  
Query: 10  DKTSHPSRLTRT---QMSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQ-N 69
           D+T+  S+L      +M+LK L C             S+     S I   P+F +LP   
Sbjct: 8   DETTQSSQLDAVWLQKMALKPLLCPPQT----TSKSFSVNETGASPIWTPPKFSKLPGVP 67

Query: 70  TQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTG 129
            + K  ST LC       + + A GV ++DG+IIVDHGSRRKESNL+LNEFV MF+ K+G
Sbjct: 68  CKTKYSSTRLCLRTGNGEITKNAHGVRERDGLIIVDHGSRRKESNLMLNEFVAMFRDKSG 127

Query: 130 YPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHP 189
           YPIVEPAHMELAEPSIKDAF  CV+QGANRVI+SPFFLFPGRHWHQDIPSLA  AA EHP
Sbjct: 128 YPIVEPAHMELAEPSIKDAFGLCVQQGANRVIVSPFFLFPGRHWHQDIPSLAAEAAKEHP 187

Query: 190 GINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
           G++Y++TAPLGLHELLVDVV DRI +CL HVAG  +ECA C GTG+CK
Sbjct: 188 GVSYIITAPLGLHELLVDVVNDRIKHCLRHVAGDADECAACVGTGKCK 231

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RXW9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_017090 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 7.900e-125
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 1


		  
Query: 23  MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 82
           MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK
Sbjct: 1   MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 60

Query: 83  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 142
           IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK
Sbjct: 61  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 120

Query: 143 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 202
           DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV
Sbjct: 121 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 180

Query: 203 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 242
           DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN
Sbjct: 181 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 219

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R808_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_096610 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 6.500e-111
Identity = 195/219 (89.04%), Postives = 203/219 (92.69%), Query Frame = 1


		  
Query: 23  MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 82
           MSLKFLSCNQWVHQLHL+SPVS TT K S IE GPR+ +LPQ+T  KSLS SLCFAMDQK
Sbjct: 1   MSLKFLSCNQWVHQLHLQSPVSSTTTKRSWIEAGPRYFKLPQSTLRKSLSPSLCFAMDQK 60

Query: 83  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 142
            +HE AFGV QKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELA+PSIK
Sbjct: 61  SLHEDAFGVGQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELADPSIK 120

Query: 143 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 202
           DAFNSCV+QGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHE LV
Sbjct: 121 DAFNSCVQQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHEFLV 180

Query: 203 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 242
           DVVKDRI YCLSHVAG+VEECAVC GTGRCK KVQDSEN
Sbjct: 181 DVVKDRIGYCLSHVAGEVEECAVCVGTGRCKLKVQDSEN 219

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FMA1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g044390 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 3.200e-102
Identity = 187/219 (85.39%), Postives = 197/219 (89.95%), Query Frame = 1


		  
Query: 23  MSLKFLSCNQWVHQLHLESPVS-LTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQ 82
           MSLK LSCNQWVH   L+SPVS L TAKGSR EV PR+   P++ Q K LS SLCF MDQ
Sbjct: 1   MSLKLLSCNQWVH---LQSPVSFLPTAKGSRTEVAPRYFNPPKSIQLKRLSPSLCFPMDQ 60

Query: 83  KIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSI 142
           K VHEGAFGV QKDGVIIVDHGSRRKESN+LLNEFVLMFKS+TGYPIVEPAHMELAEPSI
Sbjct: 61  KSVHEGAFGVGQKDGVIIVDHGSRRKESNILLNEFVLMFKSRTGYPIVEPAHMELAEPSI 120

Query: 143 KDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELL 202
           KDAF+ CV+QGAN VIISPFFLFPGRHWHQDIP LA AAALEHPGINYLVTAPLGLHELL
Sbjct: 121 KDAFDLCVQQGANCVIISPFFLFPGRHWHQDIPVLAGAAALEHPGINYLVTAPLGLHELL 180

Query: 203 VDVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSE 241
           VDVVKDRIDYCLSHVAG+VEECAVCAGTGRCKFKVQ+SE
Sbjct: 181 VDVVKDRIDYCLSHVAGQVEECAVCAGTGRCKFKVQNSE 216

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A166B8C6_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_011246 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.500e-70
Identity = 125/177 (70.62%), Postives = 149/177 (84.18%), Query Frame = 1


		  
Query: 57  PRFLRLPQNTQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEF 116
           PRF++L  N++ +++ T LCFA       +   G+  KDGVIIVDHGSRRKESNL+LNEF
Sbjct: 30  PRFVKLSNNSK-RNVPTRLCFASSNGGFRQETSGIGSKDGVIIVDHGSRRKESNLMLNEF 89

Query: 117 VLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSL 176
           V+MF+ +TGYPIVEPAHMELAEPSIKDAF SCV+QG NRVI+SPFFLFPGRHW+QDIPSL
Sbjct: 90  VIMFRDRTGYPIVEPAHMELAEPSIKDAFASCVQQGVNRVIVSPFFLFPGRHWNQDIPSL 149

Query: 177 ADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
              AA EHPG++Y++TAPLGLHELLVDVV DRI +CLSHVAG  +EC+VCAGTGRC+
Sbjct: 150 TAEAAKEHPGVSYIITAPLGLHELLVDVVNDRIQHCLSHVAGDADECSVCAGTGRCQ 205

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A067LAA8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09571 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.400e-68
Identity = 127/191 (66.49%), Postives = 148/191 (77.49%), Query Frame = 1


		  
Query: 44  SLTTAKGSRIEVGPRFLRLPQ-NTQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDH 103
           S+     S I   P+F +LP    + K  ST LC       + + A GV ++DG+IIVDH
Sbjct: 18  SVNETGASPIWTPPKFSKLPGVPCKTKYSSTRLCLRTGNGEITKNAHGVRERDGLIIVDH 77

Query: 104 GSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFF 163
           GSRRKESNL+LNEFV MF+ K+GYPIVEPAHMELAEPSIKDAF  CV+QGANRVI+SPFF
Sbjct: 78  GSRRKESNLMLNEFVAMFRDKSGYPIVEPAHMELAEPSIKDAFGLCVQQGANRVIVSPFF 137

Query: 164 LFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEE 223
           LFPGRHWHQDIPSLA  AA EHPG++Y++TAPLGLHELLVDVV DRI +CL HVAG  +E
Sbjct: 138 LFPGRHWHQDIPSLAAEAAKEHPGVSYIITAPLGLHELLVDVVNDRIKHCLRHVAGDADE 197

Query: 224 CAVCAGTGRCK 234
           CA C GTG+CK
Sbjct: 198 CAACVGTGKCK 208

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: SIRB_ARATH (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana GN=SIRB PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 4.000e-61
Identity = 110/160 (68.75%), Postives = 126/160 (78.75%), Query Frame = 1


		  
Query: 74  SLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAH 133
           S CF   + +V  G   +   DG+IIVDHGSRR+ESNL+L EFV MFK KTGYPIVEPAH
Sbjct: 64  SPCFGESEGLVKNG---IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAH 123

Query: 134 MELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTA 193
           MELAEPSIKDAF+ CV+QGA RV++SPFFLFPGRHWH DIPSL   AA E  GI+YL+TA
Sbjct: 124 MELAEPSIKDAFSLCVQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITA 183

Query: 194 PLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
           PLG H LL+DVV DRI +CLSHV G  +EC VCAGT +CK
Sbjct: 184 PLGPHNLLLDVVNDRIQHCLSHVEGDADECLVCAGTNKCK 220

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: SIRB_BACME (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium GN=sirB PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.500e-15
Identity = 46/132 (34.85%), Postives = 64/132 (48.48%), Query Frame = 1


		  
Query: 79  MDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAE 138
           M +K+  E    V   D V+ V HGSR KE       F+   K     PI E   +ELA 
Sbjct: 1   MHKKLTKE----VDYMDAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELAS 60

Query: 139 PSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLH 198
           P+I+  F +C+EQGA R+ I P  L    H   DIP         +P +  L   P G+ 
Sbjct: 61  PTIEQGFEACIEQGATRIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVD 120

Query: 199 ELLVDVVKDRID 211
           E +VD++ +RI+
Sbjct: 121 ERIVDILVERIN 128

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: CBIX_BACME (Sirohydrochlorin cobaltochelatase OS=Bacillus megaterium GN=cbiX PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 9.000e-13
Identity = 40/130 (30.77%), Postives = 66/130 (50.77%), Query Frame = 1


		  
Query: 97  VIIVDHGSRRKESNLLLNEFVLMFKSKTGYPI-VEPAHMELAEPSIKDAFNSCVEQGANR 156
           V+ V HGSR  E N    EF+   K      I VE   +E   P++    ++CV +GA  
Sbjct: 9   VLFVGHGSRDPEGND--REFISTMKHDWDASILVETCFLEFERPNVSQGIDTCVAKGAQD 68

Query: 157 VIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSH 216
           V++ P  L P  H    IP+  D A  ++P +N++   P+G+HE  ++++K R+     +
Sbjct: 69  VVVIPIMLLPAGHSKIHIPAAIDEAKEKYPHVNFVYGRPIGVHEEALEILKTRLQESGEN 128

Query: 217 VAGKVEECAV 226
           +    E+ AV
Sbjct: 129 LETPAEDTAV 136

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: CBIX_METMP (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) GN=cbiX PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.200e-12
Identity = 41/139 (29.50%), Postives = 65/139 (46.76%), Query Frame = 1


		  
Query: 95  DGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGAN 154
           + +++V HGSR   S  ++ E     K++  Y IVE   ME  EP+I       ++ GA 
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 155 RVIISPFFLFPGRHWHQDIPSLA------------------------DAAALEHP-GINY 209
           +VI++P FL PG H  +DIP +                         D  A+E P G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: SIRB_BACSU (Sirohydrochlorin ferrochelatase OS=Bacillus subtilis (strain 168) GN=sirB PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.600e-12
Identity = 36/116 (31.03%), Postives = 57/116 (49.14%), Query Frame = 1


		  
Query: 94  KDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGA 153
           K  ++ V HGSR K++      F+   K+    P+ E + +EL EP+I+  F +CV+QGA
Sbjct: 2   KQAILYVGHGSRVKKAQQEAAAFLEGCKAHISVPVQEISFLELQEPTIETGFEACVKQGA 61

Query: 154 NRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLVDVVKDRI 210
             + + P  L    H   DIP      A  +P +      P+G+ E +V  V  R+
Sbjct: 62  THIAVVPLLLLTAAHAKHDIPEEIVRVASRYPSVRISYGKPIGIDEEVVKAVYHRM 117

[Close]

BLAST of Spo22007.1 vs. TAIR (Arabidopsis)
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B)

HSP 1 Score: 236.1 bits (601), Expect = 2.200e-62
Identity = 110/160 (68.75%), Postives = 126/160 (78.75%), Query Frame = 1


		  
Query: 74  SLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAH 133
           S CF   + +V  G   +   DG+IIVDHGSRR+ESNL+L EFV MFK KTGYPIVEPAH
Sbjct: 64  SPCFGESEGLVKNG---IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAH 123

Query: 134 MELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTA 193
           MELAEPSIKDAF+ CV+QGA RV++SPFFLFPGRHWH DIPSL   AA E  GI+YL+TA
Sbjct: 124 MELAEPSIKDAFSLCVQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITA 183

Query: 194 PLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
           PLG H LL+DVV DRI +CLSHV G  +EC VCAGT +CK
Sbjct: 184 PLGPHNLLLDVVNDRIQHCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:

BLAST of Spo22007.1 vs. NCBI nr
BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
BLAST of Spo22007.1 vs. TAIR (Arabidopsis)

BLAST of Spo22007.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902236265\|gb\|KNA24303.1\|	1.1e-124	100.	hypothetical protein SOVF_0170...	[more]
gi\|902207097\|gb\|KNA15620.1\|	9.3e-111	89.0	hypothetical protein SOVF_0966...	[more]
gi\|731320521\|ref\|XP_010670852.1\|	4.6e-102	85.3	PREDICTED: uncharacterized pro...	[more]
gi\|1021044711\|gb\|KZN02492.1\|	3.6e-70	70.6	hypothetical protein DCAR_0112...	[more]
gi\|802542391\|ref\|XP_012081294.1\|	5.2e-69	59.6	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RXW9_SPIOL	7.9e-125	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9R808_SPIOL	6.5e-111	89.0	Uncharacterized protein OS=Spi...	[more]
A0A0J8FMA1_BETVU	3.2e-102	85.3	Uncharacterized protein OS=Bet...	[more]
A0A166B8C6_DAUCA	2.5e-70	70.6	Uncharacterized protein OS=Dau...	[more]
A0A067LAA8_JATCU	1.4e-68	66.4	Uncharacterized protein OS=Jat...	[more]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
SIRB_ARATH	4.0e-61	68.7	Sirohydrochlorin ferrochelatas...	[more]
SIRB_BACME	2.5e-15	34.8	Sirohydrochlorin ferrochelatas...	[more]
CBIX_BACME	9.0e-13	30.7	Sirohydrochlorin cobaltochelat...	[more]
CBIX_METMP	1.2e-12	29.5	Sirohydrochlorin cobaltochelat...	[more]
SIRB_BACSU	7.6e-12	31.0	Sirohydrochlorin ferrochelatas...	[more]

BLAST of Spo22007.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G50170.1	2.2e-62	68.7	sirohydrochlorin ferrochelatas...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002762	Cobalamin (vitamin B12) biosynthesis CbiX	PFAM	PF01903	CbiX	coord: 102..206 score: 1.0
None	No IPR available	GENE3D	3.40.50.1400		coord: 93..215 score: 6.2
None	No IPR available	PANTHER	PTHR33542	FAMILY NOT NAMED	coord: 74..234 score: 3.2
None	No IPR available	unknown	SSF53800	Chelatase	coord: 94..210 score: 2.22

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009236	cobalamin biosynthetic process
biological_process	GO:0006952	defense response
biological_process	GO:0019354	siroheme biosynthetic process
biological_process	GO:0006783	heme biosynthetic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0005982	starch metabolic process
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0009505	plant-type cell wall
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0043022	ribosome binding
molecular_function	GO:0043023	ribosomal large subunit binding
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0016887	ATPase activity
molecular_function	GO:0051266	sirohydrochlorin ferrochelatase activity
molecular_function	GO:0016852	sirohydrochlorin cobaltochelatase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo17236	0.68	Barchart \| Table
Spo13934	0.67	Barchart \| Table
Spo27360	0.67	Barchart \| Table