Spo21344 (gene)

Overview
NameSpo21344
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionPIF1-like helicase
LocationSpoScf_00928 : 21800 .. 24547 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGCTTCAAGCAGGAAAGTCAGAGTCTAGCAATCATGACATAAAGGAATTCTCAGAGTGGATATTAAAGATCGGAGATGGTAAGGTGGGAGAGCCAAATGATGGTGAAGGAACTATAGTAATCCCTGATGAAAATCTCATAAAGGAAGTAGAAAATCCAGTTGAAGCTATTGTAGAAAGTACTTACCCTTCCATTCTACATAATTTATGGGATCCAAAATACTTTCAAGAAAGAGCAATTCTAGCCCCAACCCATGATAATGTACAAATTATCAATGACTACTTGCTATCTTTAATACCAGGTGAAGAAAAAATCTACCTGAGTTCTGACAGCATATGTAAGACAGGTGCAAACATGGGAATCGAGGATCTCTACCCACTTGAGTTCCTAAATACCATTAGATGCTATGGGGTACCAAATCACATTATCAAATTGAAGGTTGGTGTTCCCGTCATGCTTCTTAGAAATATAGATCAGTCTTCTAGCTTATGCAATGGTACCAGATTGGTGATCACCCAACTTGGAAACCATGTTGTTGAAGCTAAAATAATTTCTGGTAGCAATATAGGGCTTAAGGTCTACATTCCTCGAATGACCTTAACCCCCTCAGATCCAACCAGTTTACCAATCAAGCTTCAACGATGACAATTTCCATTGGTCGTATGTTTTGCTATGACAATAAATAAAAGCCAAGGCCAGTCACTTACACATGTTGGTTTGTACCTTCCAAAACCGGTTTTCAGTCATGGTCAACTCTATGTTGCAATCTCCAGGGTGACAAGGAAGGAAGGGCTGAAAATTCTAATATGTGATGAAGCTAATGAACTTTCTGATACTACAAACAATGTAGTATACAAAGAGGTCTTTCAAAACTTGCCATGAAGAGGTATTTTATGATACAATTTCTTCTAGAAAATACTGCTTTCATTATTTTATATTTAAGGATTAGATTAATTATCTCAAATCATGAACCACGTTATTTAATTTTATTTTTGTTCATTTATTTTCATGCAGTGGCAGTCGAAGCTAAGTGTACTCGATAACAAGAAACTGGAAGTTTTTATCGCACTATAAACAACAATTTATTCTTTTGTAGCTTTTTTTTTTGTTAGGATACAAACAATTATTTACATGCTATGCGTACTAAGCAAAGAGGGTTTTTTACCATGTTATTTACAATTGGTGTTTTGTTATTTATTGAATTTCTGTTTTCAAGAATATATTCCTCCGTCTTCTTACATATTTGAAAGTACAATGATTAATCAAGTGGAATTCAATCAGCAAGAGGTAGTTCGCGCAGATTCGGGGTCAATTTTCTTGAAACATAAACATGCAAATACTTGCTTCAGCCGATAAAATAGCATCAAATACAAAAATCAGCGGTAACAACCAGATGAACAACAACTATTCTGGGCAACTATCACTGACTTATAAGACAGGGATTTTATGATTGATTATCAAATTCCAAACTATATCTAAGTACCAAAAAAAAACAAAGAACAATCACACCAACATAAAAAATTGCGAACGGAAAGGGTAATAATAATAGGAAAGAAAGAGAAATACCACTCTTTAACCCATTTCAAAGCTGGTGTTTTAGATTTTACAATTGGTCGTCTGTAGAACAAATTATGCGACATCGACATCTGAGTCTCCATCTCCTTTCTATCTATTACCACGAAATTGTGAAAATTGATGATGGCGGTTGAAATTGTGAGTATAGTAGGAGTCAAAAAGAACTTTTAGATTTTTGGCAAGACCATGATCTAGATCTTTCAAAAATGAGTAAAATGGGTAAGCTTTGTTTTTTTGATGCGATGATCGAAGTGGGATCATTGCGATAATATCAATGCTATTTTTCTTCTGGAAGTTGATCGGTTAGCTTTCCTTTTGTTTTTATTTATTTATTTTTAATTCAACAATATAATATTTACAAACTTTTGTAAAAGACGGTCTTATAGGAAAGACCATATTTTCCACTCCAATATTCGCGGGTCGATTCAAACGGATACGGGTCAGCGCGGATTTTTATGTTTCGGGTCATATAAGAACATATTTTCCACTCCATAATTCATTTTCTCTCTCCTCAAAATGCGTCTGCCCTCTCTCTCTCTCCTCTAAAACTTTCGCCTCTCTCTCCTCCAAAACTCAGGCACCTCCTCACCGCCGTCTCCGTCGCCTCCTCGGCGCCGTCTCCATTTTCTCCGACCTCCTTTTCCGACATCAAGGTTTTTGAAGCTGAATGAGGCTACGTAGGTCAAGGTACGCCGGCGAATTTTGACCCGTGAAAGTTCCTCGCCGGTGTAAGTGGCGGTGTAACAGATCGGAGGTGTGAGGATGATGTTCTGTCGCCATTCGATGATTTTGAGAATGATGCCTCCTTGCCGCCGTCGCCATCGGAGGTTAGGGTTTTTCGAAATTCTTCTCTCTCCTTCATTCATCTGGTTTAAGATTCTTCATCTTTTCGAATAAGTCTTCTTCTAATATTCTTTTGCAGTAGGTCTTGGGATCCATTAACATGATTTGATTTTGGTTTCGAATTTGTTTCTGATTTGATTTTGGTTTGTGGCGTCGAATGCGGCGTCTCCTCTTTTTGTAGATGAGATGAATATATGATGGGCAGGAGATGAATATATCATTGTGTGTTGTTGATATCGGAGAACTCGATAATTGTAACATCAACAACAAACAATTGTAGCAGTAGCACTAAATTAAATATTGGATTCTCCGTTGATACAAATGGAG

mRNA sequence

ATGAGGCTTCAAGCAGGAAAGTCAGAGTCTAGCAATCATGACATAAAGGAATTCTCAGAGTGGATATTAAAGATCGGAGATGGTAAGGTGGGAGAGCCAAATGATGGTGAAGGAACTATAGTAATCCCTGATGAAAATCTCATAAAGGAAGTAGAAAATCCAGTTGAAGCTATTGTAGAAAGTACTTACCCTTCCATTCTACATAATTTATGGGATCCAAAATACTTTCAAGAAAGAGCAATTCTAGCCCCAACCCATGATAATGTACAAATTATCAATGACTACTTGCTATCTTTAATACCAGGTGAAGAAAAAATCTACCTGAGTTCTGACAGCATATGTAAGACAGGTGCAAACATGGGAATCGAGGATCTCTACCCACTTGAGTTCCTAAATACCATTAGATGCTATGGGGTACCAAATCACATTATCAAATTGAAGGTTGGTGTTCCCGTCATGCTTCTTAGAAATATAGATCAGTCTTCTAGCTTATGCAATGGTACCAGATTGGTGATCACCCAACTTGGAAACCATGTTGTTGAAGCTAAAATAATTTCTGGTAGCAATATAGGGCTTAAGGCACCTCCTCACCGCCGTCTCCGTCGCCTCCTCGGCGCCGTCTCCATTTTCTCCGACCTCCTTTTCCGACATCAAGATCGGAGGTGTGAGGATGATGTTCTGTCGCCATTCGATGATTTTGAGAATGATGCCTCCTTGCCGCCGTCGCCATCGGAGATGAGATGAATATATGATGGGCAGGAGATGAATATATCATTGTGTGTTGTTGATATCGGAGAACTCGATAATTGTAACATCAACAACAAACAATTGTAGCAGTAGCACTAAATTAAATATTGGATTCTCCGTTGATACAAATGGAG

Coding sequence (CDS)

ATGAGGCTTCAAGCAGGAAAGTCAGAGTCTAGCAATCATGACATAAAGGAATTCTCAGAGTGGATATTAAAGATCGGAGATGGTAAGGTGGGAGAGCCAAATGATGGTGAAGGAACTATAGTAATCCCTGATGAAAATCTCATAAAGGAAGTAGAAAATCCAGTTGAAGCTATTGTAGAAAGTACTTACCCTTCCATTCTACATAATTTATGGGATCCAAAATACTTTCAAGAAAGAGCAATTCTAGCCCCAACCCATGATAATGTACAAATTATCAATGACTACTTGCTATCTTTAATACCAGGTGAAGAAAAAATCTACCTGAGTTCTGACAGCATATGTAAGACAGGTGCAAACATGGGAATCGAGGATCTCTACCCACTTGAGTTCCTAAATACCATTAGATGCTATGGGGTACCAAATCACATTATCAAATTGAAGGTTGGTGTTCCCGTCATGCTTCTTAGAAATATAGATCAGTCTTCTAGCTTATGCAATGGTACCAGATTGGTGATCACCCAACTTGGAAACCATGTTGTTGAAGCTAAAATAATTTCTGGTAGCAATATAGGGCTTAAGGCACCTCCTCACCGCCGTCTCCGTCGCCTCCTCGGCGCCGTCTCCATTTTCTCCGACCTCCTTTTCCGACATCAAGATCGGAGGTGTGAGGATGATGTTCTGTCGCCATTCGATGATTTTGAGAATGATGCCTCCTTGCCGCCGTCGCCATCGGAGATGAGATGA

Protein sequence

MRLQAGKSESSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVESTYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANMGIEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVVEAKIISGSNIGLKAPPHRRLRRLLGAVSIFSDLLFRHQDRRCEDDVLSPFDDFENDASLPPSPSEMR
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo21344.1Spo21344.1mRNA


Homology
[Close]
BLAST of Spo21344.1 vs. NCBI nr
Match: gi|731362272|ref|XP_010692805.1| (PREDICTED: uncharacterized protein LOC104905864 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 258.1 bits (658), Expect = 1.600e-65
Identity = 119/193 (61.66%), Postives = 153/193 (79.27%), Query Frame = 1 

		  

Query: 1    MRLQAGKSESSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVE 60
            MRLQ G SE+S  ++KEFSEWIL +GDG  G PN+GE  I + ++ LI+   +P+ AIVE
Sbjct: 1219 MRLQTGSSETSLKEVKEFSEWILSVGDGNAGGPNEGEAEIKVSNDILIEGESDPIAAIVE 1278

Query: 61   STYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANM 120
            STYP +  +LW+PKYFQERAILAPT++ V+++NDY+LS +PGEEK+YLSSD+I     N+
Sbjct: 1279 STYPLLKDHLWEPKYFQERAILAPTYEIVEMVNDYVLSHLPGEEKLYLSSDAISNVEGNL 1338

Query: 121  GIEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVV 180
            G  ++Y  EFLNTIRC G+PNH ++LKVG PVMLLRNIDQ+S LCNGTRLVI  LGN ++
Sbjct: 1339 GASEIYSTEFLNTIRCSGLPNHHLRLKVGAPVMLLRNIDQTSGLCNGTRLVIKHLGNRII 1398

Query: 181  EAKIISGSNIGLK 194
            EA +ISGSN+G K
Sbjct: 1399 EAVVISGSNVGDK 1411
[Close]
BLAST of Spo21344.1 vs. NCBI nr
Match: gi|731368767|ref|XP_010696326.1| (PREDICTED: uncharacterized protein LOC104908859 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 252.3 bits (643), Expect = 8.800e-64
Identity = 118/193 (61.14%), Postives = 153/193 (79.27%), Query Frame = 1 

		  

Query: 1    MRLQAGKSESSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVE 60
            MRLQ+  S S+ ++I++FSEWIL +GDG+ G PNDGE  I I ++ LI   ++P+ AIV+
Sbjct: 1132 MRLQSCSSSSTANEIRDFSEWILSVGDGRAGGPNDGEVDIEITEDILIDGGDDPISAIVD 1191

Query: 61   STYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANM 120
            STYPS+  +LW+ KYFQERAILAPT++ V+ +ND++LS IPG+EKIYLS D+I K   N+
Sbjct: 1192 STYPSLTDHLWEAKYFQERAILAPTNEIVEKVNDHVLSTIPGDEKIYLSCDAISKDEGNL 1251

Query: 121  GIEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVV 180
            G  D+Y  EFLNTI+C G+PNH IKLKVG P+MLLRNIDQ+S LCNGTRLV+  LGN V+
Sbjct: 1252 GAHDIYSTEFLNTIKCSGLPNHSIKLKVGAPIMLLRNIDQTSGLCNGTRLVVKHLGNRVI 1311

Query: 181  EAKIISGSNIGLK 194
            EA +ISGSN+G K
Sbjct: 1312 EATVISGSNVGDK 1324
[Close]
BLAST of Spo21344.1 vs. NCBI nr
Match: gi|731330198|ref|XP_010675998.1| (PREDICTED: ATP-dependent DNA helicase PIF2-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 252.3 bits (643), Expect = 8.800e-64
Identity = 118/193 (61.14%), Postives = 153/193 (79.27%), Query Frame = 1 

		  

Query: 1   MRLQAGKSESSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVE 60
           MRLQ+  S S+ ++I++FSEWIL +GDG+ G PNDGE  I I ++ LI   ++P+ AIV+
Sbjct: 296 MRLQSCSSSSTANEIRDFSEWILSVGDGRAGGPNDGEVDIEITEDILIDGGDDPISAIVD 355

Query: 61  STYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANM 120
           STYPS+  +LW+ KYFQERAILAPT++ V+ +ND++LS IPG+EKIYLS D+I K   N+
Sbjct: 356 STYPSLTDHLWEAKYFQERAILAPTNEIVEKVNDHVLSTIPGDEKIYLSCDAISKDEGNL 415

Query: 121 GIEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVV 180
           G  D+Y  EFLNTI+C G+PNH IKLKVG P+MLLRNIDQ+S LCNGTRLV+  LGN V+
Sbjct: 416 GAHDIYSTEFLNTIKCSGLPNHSIKLKVGAPIMLLRNIDQTSGLCNGTRLVVKHLGNRVI 475

Query: 181 EAKIISGSNIGLK 194
           EA +ISGSN+G K
Sbjct: 476 EATVISGSNVGDK 488
[Close]
BLAST of Spo21344.1 vs. NCBI nr
Match: gi|731355483|ref|XP_010689165.1| (PREDICTED: uncharacterized protein LOC104902921 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 250.8 bits (639), Expect = 2.600e-63
Identity = 117/193 (60.62%), Postives = 150/193 (77.72%), Query Frame = 1 

		  

Query: 1    MRLQAGKSESSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVE 60
            MRLQ G SE+S  ++KEFSEWIL +GDG  G PN+GE  I + ++ LI+   + + AIVE
Sbjct: 1130 MRLQNGSSETSLKEVKEFSEWILSVGDGNAGGPNEGEAEIKVSNDILIEGESSLIAAIVE 1189

Query: 61   STYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANM 120
            STYP +  +LW+PKYFQERAI APT++ V+ +NDY+LS +PGEEK+YLSSD+I     N+
Sbjct: 1190 STYPLLKDHLWEPKYFQERAIFAPTYEIVETVNDYVLSHLPGEEKLYLSSDAISNVEGNL 1249

Query: 121  GIEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVV 180
            G  ++Y  EFLNTIRC G+PNH ++LKVG PVMLLRNIDQ+S LCNGTR+VI  LGN V+
Sbjct: 1250 GASEIYSTEFLNTIRCSGLPNHHLRLKVGAPVMLLRNIDQTSGLCNGTRMVIKHLGNRVI 1309

Query: 181  EAKIISGSNIGLK 194
            EA +ISGSN+G K
Sbjct: 1310 EAVVISGSNVGDK 1322
[Close]
BLAST of Spo21344.1 vs. NCBI nr
Match: gi|731356038|ref|XP_010689458.1| (PREDICTED: ATP-dependent DNA helicase PIF1-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 250.4 bits (638), Expect = 3.300e-63
Identity = 115/193 (59.59%), Postives = 150/193 (77.72%), Query Frame = 1 

		  

Query: 1   MRLQAGKSESSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVE 60
           MRL+ G +     +I+ FSEWIL IGDG+ GEPNDGEG + IPD+ LI   ++P+ A+V+
Sbjct: 102 MRLRPGSNNRDVVEIRNFSEWILNIGDGEAGEPNDGEGCVEIPDDILIDGGDDPIAAVVD 161

Query: 61  STYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANM 120
           +TYP +  +LW+  YFQERAILAPTHD V+ +ND++LS +PGEE+ YLSSD+I K  AN 
Sbjct: 162 NTYPDLEAHLWEATYFQERAILAPTHDIVETVNDHVLSQLPGEERTYLSSDAISKEEANF 221

Query: 121 GIEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVV 180
           G+ ++Y  EFLNTI+C G+PNH ++LKVG PVMLLRNIDQ+  LCNGTRLV+  LGN +V
Sbjct: 222 GVHEMYSTEFLNTIKCSGLPNHNMRLKVGAPVMLLRNIDQTYGLCNGTRLVVKHLGNRIV 281

Query: 181 EAKIISGSNIGLK 194
           EA +ISGSN+G K
Sbjct: 282 EAVVISGSNVGDK 294
[Close]
BLAST of Spo21344.1 vs. UniProtKB/TrEMBL
Match: A0A151T5C9_CAJCA (ATP-dependent DNA helicase PIF1 OS=Cajanus cajan GN=KK1_016756 PE=4 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.500e-54
Identity = 107/192 (55.73%), Postives = 147/192 (76.56%), Query Frame = 1 

		  

Query: 1   MRLQAGKSESSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVE 60
           MRL   K++++  DIK+FS+WIL+IGDG +   ++GE  I IP E LI+ VENP+ ++V+
Sbjct: 1   MRLNTTKTDATTFDIKKFSDWILQIGDGDMLMDHNGEAEISIPKELLIQPVENPLMSLVQ 60

Query: 61  STYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANM 120
             YPSILHN+ D  +FQERAILAPT ++ + +ND++LSLIPGEEKIYLSSD+ C +    
Sbjct: 61  FVYPSILHNMKDVNFFQERAILAPTVESFEQVNDFMLSLIPGEEKIYLSSDTPCPSDEKT 120

Query: 121 GIE-DLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHV 180
            I+ + +  EFLN ++ YG+PNH + LKVGVPVMLLRN+DQS+ LCNGTRL+IT LG +V
Sbjct: 121 EIQGEWFISEFLNDVKYYGIPNHKLTLKVGVPVMLLRNLDQSNGLCNGTRLLITDLGTNV 180

Query: 181 VEAKIISGSNIG 192
           + A +I+ +NIG
Sbjct: 181 ISATVITDTNIG 192
[Close]
BLAST of Spo21344.1 vs. UniProtKB/TrEMBL
Match: A0A0R0K9R8_SOYBN (Uncharacterized protein (Fragment) OS=Glycine max GN=GLYMA_04G183200 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.300e-53
Identity = 104/193 (53.89%), Postives = 147/193 (76.17%), Query Frame = 1 

		  

Query: 1    MRLQAGKSESSN-HDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIV 60
            MRLQ+  ++ SN  ++K+FSEW+L IGDGK+ EPNDG G I IPDE LIK+ ++P++AIV
Sbjct: 1092 MRLQSNPTDHSNLDELKQFSEWLLDIGDGKLAEPNDGYGEITIPDEFLIKDFQDPIQAIV 1151

Query: 61   ESTYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGAN 120
            E+TYP++LHN  +  + Q+R + A T D V  INDY+LSLIPGEEK Y S+DS+ K+   
Sbjct: 1152 EATYPNLLHNSSNGDFLQKRVVFASTKDVVDKINDYVLSLIPGEEKEYCSADSVDKSDEL 1211

Query: 121  MG-IEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNH 180
            +     +   EFLN+++  G+PNH +++KVG P++LLRN+DQ+  LCNGTRL++T+LG+ 
Sbjct: 1212 LSPAFGVLTAEFLNSLKTSGIPNHKLRIKVGTPIILLRNLDQADGLCNGTRLIVTRLGSS 1271

Query: 181  VVEAKIISGSNIG 192
            VVEA+II+G NIG
Sbjct: 1272 VVEAEIITGPNIG 1284
[Close]
BLAST of Spo21344.1 vs. UniProtKB/TrEMBL
Match: K7KKX7_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.300e-53
Identity = 104/193 (53.89%), Postives = 147/193 (76.17%), Query Frame = 1 

		  

Query: 1    MRLQAGKSESSN-HDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIV 60
            MRLQ+  ++ SN  ++K+FSEW+L IGDGK+ EPNDG G I IPDE LIK+ ++P++AIV
Sbjct: 961  MRLQSNPTDHSNLDELKQFSEWLLDIGDGKLAEPNDGYGEITIPDEFLIKDFQDPIQAIV 1020

Query: 61   ESTYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGAN 120
            E+TYP++LHN  +  + Q+R + A T D V  INDY+LSLIPGEEK Y S+DS+ K+   
Sbjct: 1021 EATYPNLLHNSSNGDFLQKRVVFASTKDVVDKINDYVLSLIPGEEKEYCSADSVDKSDEL 1080

Query: 121  MG-IEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNH 180
            +     +   EFLN+++  G+PNH +++KVG P++LLRN+DQ+  LCNGTRL++T+LG+ 
Sbjct: 1081 LSPAFGVLTAEFLNSLKTSGIPNHKLRIKVGTPIILLRNLDQADGLCNGTRLIVTRLGSS 1140

Query: 181  VVEAKIISGSNIG 192
            VVEA+II+G NIG
Sbjct: 1141 VVEAEIITGPNIG 1153
[Close]
BLAST of Spo21344.1 vs. UniProtKB/TrEMBL
Match: Q8RV60_ARATH (Putative uncharacterized protein At2g05640 OS=Arabidopsis thaliana GN=At2g05640 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.200e-53
Identity = 101/193 (52.33%), Postives = 142/193 (73.58%), Query Frame = 1 

		  

Query: 1    MRLQAGKSESSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVE 60
            MRL A  +E    +IKEFS+WILK+GDGK+ EPNDGE  I IP++ L+ +  +P+++I  
Sbjct: 1012 MRLTADPTEKD--NIKEFSDWILKLGDGKLSEPNDGEAAIDIPEDMLLLDSLHPIDSIAN 1071

Query: 61   STYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANM 120
              YP++L NL D  +F+ERAIL PT+D+V  +N++++ L+PGE K Y SSD IC    ++
Sbjct: 1072 CVYPNLLQNLNDQTFFRERAILCPTNDDVSEVNNHIMDLLPGEVKEYFSSDKICDFDTSV 1131

Query: 121  GIEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVV 180
              +     EFLN I+C GVPNH+++LK+GVPVML+RN+DQ   LCNGTRL +TQLG+ V+
Sbjct: 1132 ERDANMSTEFLNAIKCSGVPNHVLRLKLGVPVMLIRNLDQKYGLCNGTRLQVTQLGDRVI 1191

Query: 181  EAKIISGSNIGLK 194
            EAK+++GSN G K
Sbjct: 1192 EAKVLTGSNAGNK 1202
[Close]
BLAST of Spo21344.1 vs. UniProtKB/TrEMBL
Match: A0A0R0FZX5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_16G087000 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.100e-52
Identity = 101/193 (52.33%), Postives = 145/193 (75.13%), Query Frame = 1 

		  

Query: 1    MRLQAGKSE-SSNHDIKEFSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIV 60
            M LQ+  S  SS +++K+FS+W+L IGDGK+G+PNDG   I+IPDE LI + E+P++AIV
Sbjct: 1188 MCLQSNPSNHSSTYELKQFSDWLLDIGDGKLGQPNDGHCKIIIPDEFLIMDFEDPIQAIV 1247

Query: 61   ESTYPSILHNLWDPKYFQERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGAN 120
            ++TYP +L N  +  + Q+RAILA T D V  INDY+L LIPG+EK Y S DS+ K+   
Sbjct: 1248 DATYPDLLQNYNNANFLQKRAILASTKDVVDKINDYVLCLIPGDEKEYCSVDSVDKSDEL 1307

Query: 121  MG-IEDLYPLEFLNTIRCYGVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNH 180
            +     +  LEFLNT++  G+PNH +++K+G P+MLLRN+DQ+  LCNGTRL++T+LG +
Sbjct: 1308 LNPAFGVLTLEFLNTLKTSGIPNHKLRIKIGTPIMLLRNLDQADGLCNGTRLIVTKLGTN 1367

Query: 181  VVEAKIISGSNIG 192
            VVEA++I+G N+G
Sbjct: 1368 VVEAEVITGPNVG 1380
[Close]
BLAST of Spo21344.1 vs. TAIR (Arabidopsis)
Match: AT3G51700.1 (PIF1 helicase)

HSP 1 Score: 147.5 bits (371), Expect = 1.100e-35
Identity = 78/176 (44.32%), Postives = 112/176 (63.64%), Query Frame = 1 

		  

Query: 18  FSEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVESTYPSILHNLWDPKYFQ 77
           F++WI  IG   + +PNDGE  I I ++ LI E ++P++ IV+  Y       ++P ++Q
Sbjct: 60  FTKWITNIGGENINKPNDGETKIDIHEDLLITECKDPIKTIVDEVYGESFTESYNPDFYQ 119

Query: 78  ERAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANMGIEDLYPLEFLNTIRCY 137
           ERAIL  T+D    INDY+LS + GEE     +D+I  T A+   + LYPLEFLN+I+  
Sbjct: 120 ERAILCHTNDVADEINDYMLSQLQGEETKCYGADTIYPTHASPNDKMLYPLEFLNSIKIP 179

Query: 138 GVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVVEAKIISGSNIGLK 194
           G P+  ++LKVG PVMLLR++     L  GTRL IT++   V+EA II+G+N G K
Sbjct: 180 GFPDFKLRLKVGAPVMLLRDLAPYGWLRKGTRLQITRVETFVLEAMIITGNNHGEK 235
[Close]
BLAST of Spo21344.1 vs. TAIR (Arabidopsis)
Match: AT3G51690.1 (PIF1 helicase)

HSP 1 Score: 136.3 bits (342), Expect = 2.500e-32
Identity = 74/176 (42.05%), Postives = 109/176 (61.93%), Query Frame = 1 

		  

Query: 19  SEWILKIGDGKVGEPNDGEGTIVIPDENLIKEVENPVEAIVESTYPSILHNLWDPKYFQE 78
           S WI+ I    +  PNDGE  I I  + LI E ++P++ +++  Y       ++P +  +
Sbjct: 60  SRWIMNISGDNINNPNDGETEIDISKDLLITESKDPIKTLLKEVYGEYFAKSYNPDFCHD 119

Query: 79  RAILAPTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGANMGIEDLY-PLEFLNTIRCY 138
            AIL    D+V  INDY+LSL+PGEEK  LS+DSI  +      +D++ PLE LN+I+  
Sbjct: 120 SAILCHRDDDVDQINDYMLSLLPGEEKECLSTDSISPSPN----DDMFVPLEVLNSIKVP 179

Query: 139 GVPNHIIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVVEAKIISGSNIGLK 194
           G+P+  ++LKVG PVMLLR++D S     GTRL IT+L   ++EA II+G+  G K
Sbjct: 180 GLPDFKLRLKVGAPVMLLRDLDPSRGFFTGTRLQITRLCGFLLEAMIIAGNKHGKK 231
[Close]
BLAST of Spo21344.1 vs. TAIR (Arabidopsis)
Match: AT5G28780.1 (PIF1 helicase)

HSP 1 Score: 115.2 bits (287), Expect = 5.900e-26
Identity = 62/169 (36.69%), Postives = 97/169 (57.40%), Query Frame = 1 

		  

Query: 25  IGDGKVGEPNDGEGTIVIPDENL-IKEVENPVEAIVESTYPSILHNLWDPKYFQERAILA 84
           +G+G        +G +V  D +  +K   N ++ + +  Y     +  + +Y  ER IL 
Sbjct: 63  VGNGNAPTCETDDGEVVEMDTSFFLKHNGNRLQQVTKGAYVQFSVSQPNFQYLTERGILT 122

Query: 85  PTHDNVQIINDYLLSLIPGEEKIYLSSDSICKTGA-NMGIEDLYPLEFLNTIRCYGVPNH 144
           P ++ V  IN Y+LS + G+ K YLSS SI K        E LY +++LN++    +P H
Sbjct: 123 PHNEYVDEINAYMLSQVGGDSKEYLSSYSIGKADTIGADYEALYHVKYLNSLEFPSLPKH 182

Query: 145 IIKLKVGVPVMLLRNIDQSSSLCNGTRLVITQLGNHVVEAKIISGSNIG 192
            I LK GVP+M +RN +Q   LCNGTRL++T LG  V+EA+I++G++ G
Sbjct: 183 KISLKKGVPIMQMRNFNQKEGLCNGTRLIVTNLGEQVIEAQIVTGTHAG 231
The following BLAST results are available for this feature:
BLAST of Spo21344.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|731362272|ref|XP_010692805.1|1.6e-6561.6PREDICTED: uncharacterized pro... [more]
gi|731368767|ref|XP_010696326.1|8.8e-6461.1PREDICTED: uncharacterized pro... [more]
gi|731330198|ref|XP_010675998.1|8.8e-6461.1PREDICTED: ATP-dependent DNA h... [more]
gi|731355483|ref|XP_010689165.1|2.6e-6360.6PREDICTED: uncharacterized pro... [more]
gi|731356038|ref|XP_010689458.1|3.3e-6359.5PREDICTED: ATP-dependent DNA h... [more]
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BLAST of Spo21344.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A151T5C9_CAJCA1.5e-5455.7ATP-dependent DNA helicase PIF... [more]
A0A0R0K9R8_SOYBN1.3e-5353.8Uncharacterized protein (Fragm... [more]
K7KKX7_SOYBN1.3e-5353.8Uncharacterized protein OS=Gly... [more]
Q8RV60_ARATH2.2e-5352.3Putative uncharacterized prote... [more]
A0A0R0FZX5_SOYBN4.1e-5252.3Uncharacterized protein OS=Gly... [more]
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BLAST of Spo21344.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo21344.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3
Match NameE-valueIdentityDescription
AT3G51700.11.1e-3544.3PIF1 helicase[more]
AT3G51690.12.5e-3242.0PIF1 helicase[more]
AT5G28780.15.9e-2636.6PIF1 helicase[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010285DNA helicase Pif1-likePFAMPF05970PIF1coord: 1..175
score: 4.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 140..194
score: 2.23E-11coord: 75..107
score: 2.23
NoneNo IPR availablePANTHERPTHR10492UNCHARACTERIZEDcoord: 1..191
score: 4.5E

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
biological_process GO:0000723 telomere maintenance
biological_process GO:0006886 intracellular protein transport
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009069 serine family amino acid metabolic process
cellular_component GO:0005657 replication fork
molecular_function GO:0003678 DNA helicase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004721 phosphoprotein phosphatase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004674 protein serine/threonine kinase activity
RNA-Seq Expression