Spo20562 (gene)

BLAST of Spo20562.1 vs. NCBI nr
Match: gi|902186669|gb|KNA10757.1| (hypothetical protein SOVF_141420 [Spinacia oleracea])

HSP 1 Score: 386.7 bits (992), Expect = 2.400e-104
Identity = 198/200 (99.00%), Postives = 198/200 (99.00%), Query Frame = 1

		  

Query: 1   MAGRHGRKYVKDEEFEEEEVWGVMEENSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMI 60
           MAGRHGRKYVKDEEFEEEEVWGVMEENSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMI
Sbjct: 1   MAGRHGRKYVKDEEFEEEEVWGVMEENSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMI 60

Query: 61  PRSSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYG 120
           PRSSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYG
Sbjct: 61  PRSSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYG 120

Query: 121 HGCHGHVNHGDGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTL 180
           HGCHGHVNHGDGSDEDEDGDK  DDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTL
Sbjct: 121 HGCHGHVNHGDGSDEDEDGDK--DDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTL 180

Query: 181 KGRDLCKVRNSILTKTGFLE 201
           KGRDLCKVRNSILTKTGFLE
Sbjct: 181 KGRDLCKVRNSILTKTGFLE 198

		  

BLAST of Spo20562.1 vs. NCBI nr
Match: gi|731351060|ref|XP_010686835.1| (PREDICTED: probable serine/threonine-protein kinase DDB_G0272282 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 228.4 bits (581), Expect = 1.100e-56
Identity = 145/215 (67.44%), Postives = 168/215 (78.14%), Query Frame = 1

		  

Query: 1   MAGRHGRKYVKDEEFEEEEVWGVMEE--NSSPKLRKLPLK-----SSSATKHFTAVTGGN 60
           MAGR+GRKY+KDE+FEE EVW VMEE  NSSPKL+K P K     SSSATKHFT +    
Sbjct: 1   MAGRYGRKYLKDEDFEEGEVWNVMEERENSSPKLKK-PFKNSSFLSSSATKHFTTMA--- 60

Query: 61  NSAARMIPRSSTSSSS-LLSSPSSCGKQSSAPMDIPDWSKIYGK----KNNG--ASSWVD 120
           ++A RMIPRSS+SSSS +++S SS  KQSSAP++IPDWSKIYGK    K NG  +SSWVD
Sbjct: 61  SAAPRMIPRSSSSSSSSVVASSSSSEKQSSAPVEIPDWSKIYGKTSKKKKNGTISSSWVD 120

Query: 121 YNNAVVTTHDVYYGHGCHGHVNHG-DGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARS 180
           YN      HD YYG+G + H +H  +G  +  DGD DD DDDDD+MVPPHEYLAK+LARS
Sbjct: 121 YN---AVNHDGYYGNGGNDHHHHHLNGYYDVGDGD-DDGDDDDDDMVPPHEYLAKKLARS 180

Query: 181 KISSFSVYEGIGRTLKGRDLCKVRNSILTKTGFLE 201
           +ISSFSVYEGIGRTLKGRDL KVRN+ILTKTGFLE
Sbjct: 181 QISSFSVYEGIGRTLKGRDLSKVRNAILTKTGFLE 207

		  

BLAST of Spo20562.1 vs. NCBI nr
Match: gi|870852225|gb|KMT04169.1| (hypothetical protein BVRB_8g186170 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 152.9 bits (385), Expect = 5.900e-34
Identity = 90/128 (70.31%), Postives = 102/128 (79.69%), Query Frame = 1

		  

Query: 80  QSSAPMDIPDWSKIYGK----KNNG--ASSWVDYNNAVVTTHDVYYGHGCHGHVNHG-DG 139
           QSSAP++IPDWSKIYGK    K NG  +SSWVDYN      HD YYG+G + H +H  +G
Sbjct: 46  QSSAPVEIPDWSKIYGKTSKKKKNGTISSSWVDYN---AVNHDGYYGNGGNDHHHHHLNG 105

Query: 140 SDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLKGRDLCKVRNSI 199
             +  DGD DD DDDDD+MVPPHEYLAK+LARS+ISSFSVYEGIGRTLKGRDL KVRN+I
Sbjct: 106 YYDVGDGD-DDGDDDDDDMVPPHEYLAKKLARSQISSFSVYEGIGRTLKGRDLSKVRNAI 165

Query: 200 LTKTGFLE 201
           LTKTGFLE
Sbjct: 166 LTKTGFLE 169

		  

BLAST of Spo20562.1 vs. NCBI nr
Match: gi|1009108364|ref|XP_015884356.1| (PREDICTED: zinc-regulated protein 8 [Ziziphus jujuba])

HSP 1 Score: 152.5 bits (384), Expect = 7.700e-34
Identity = 101/199 (50.75%), Postives = 134/199 (67.34%), Query Frame = 1

		  

Query: 6   GRKYVKDEEFEEEEVWGVMEEN--SSPKLRKLPLKSSSATKHFTAVTGGNNSAARMIPRS 65
           G   ++DE+FEEE+VWG ++E+  SSP  RK    SS+++  +       +SA RMIPR 
Sbjct: 9   GGTSMRDEDFEEEDVWGFVKESKDSSPITRKSKEYSSTSSSAWRI-----HSAPRMIPRV 68

Query: 66  STSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYGHGC 125
           +T ++       +  +QSSAP+DIPDWSKIYGK      SWV+ N       D ++G+  
Sbjct: 69  NTQTTI---EAKTHVQQSSAPVDIPDWSKIYGKNARNIGSWVNIN-------DDHHGNCA 128

Query: 126 --HGHVNHGDGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLK 185
             HG V + +G  +++  D DDDDDD D MVPPHE++A++LARS+ISSFSV EGIGRTLK
Sbjct: 129 DDHGGVVYENGHGDND--DDDDDDDDGDGMVPPHEWIARKLARSQISSFSVCEGIGRTLK 188

Query: 186 GRDLCKVRNSILTKTGFLE 201
           GRDL K+RN ILTKTGFLE
Sbjct: 189 GRDLSKMRNDILTKTGFLE 190

		  

BLAST of Spo20562.1 vs. NCBI nr
Match: gi|743885691|ref|XP_011037628.1| (PREDICTED: uncharacterized protein LOC105134789 [Populus euphratica])

HSP 1 Score: 149.1 bits (375), Expect = 8.500e-33
Identity = 99/201 (49.25%), Postives = 130/201 (64.68%), Query Frame = 1

		  

Query: 3   GRHGRKYVKDEEFEEEEVWGVMEE-NSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMIP 62
           G +GR+ ++DE+FEEE+VW V++E   S    K P    S    F++     ++A RMIP
Sbjct: 16  GGYGRRSMRDEDFEEEDVWSVVKEMGDSSSSSKYPSSGPS----FSSAWHLPSTAPRMIP 75

Query: 63  RSSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGA-SSWVDYNNAVVTTHDVYYG 122
            + T++++      +  +QSSAP++IPDWSKIYGK +    +SWVD +N +       YG
Sbjct: 76  ATKTTATN--HGGKAVHQQSSAPVNIPDWSKIYGKDSRVIMASWVDDHNGIA------YG 135

Query: 123 HGCHGHVNHG-DGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRT 182
            G   HVN   D  DED DGD          +VPPHE+LA++LARS+ISSFSV EGIGRT
Sbjct: 136 DGDDDHVNDDVDSCDEDGDGD----------IVPPHEWLARKLARSQISSFSVCEGIGRT 194

Query: 183 LKGRDLCKVRNSILTKTGFLE 201
           LKGRDL KVRN+ILTKTGFLE
Sbjct: 196 LKGRDLSKVRNAILTKTGFLE 194

		  

BLAST of Spo20562.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QTZ4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_141420 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 1.700e-104
Identity = 198/200 (99.00%), Postives = 198/200 (99.00%), Query Frame = 1

		  

Query: 1   MAGRHGRKYVKDEEFEEEEVWGVMEENSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMI 60
           MAGRHGRKYVKDEEFEEEEVWGVMEENSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMI
Sbjct: 1   MAGRHGRKYVKDEEFEEEEVWGVMEENSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMI 60

Query: 61  PRSSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYG 120
           PRSSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYG
Sbjct: 61  PRSSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYG 120

Query: 121 HGCHGHVNHGDGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTL 180
           HGCHGHVNHGDGSDEDEDGDK  DDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTL
Sbjct: 121 HGCHGHVNHGDGSDEDEDGDK--DDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTL 180

Query: 181 KGRDLCKVRNSILTKTGFLE 201
           KGRDLCKVRNSILTKTGFLE
Sbjct: 181 KGRDLCKVRNSILTKTGFLE 198

		  

BLAST of Spo20562.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BVZ4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g186170 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.100e-34
Identity = 90/128 (70.31%), Postives = 102/128 (79.69%), Query Frame = 1

		  

Query: 80  QSSAPMDIPDWSKIYGK----KNNG--ASSWVDYNNAVVTTHDVYYGHGCHGHVNHG-DG 139
           QSSAP++IPDWSKIYGK    K NG  +SSWVDYN      HD YYG+G + H +H  +G
Sbjct: 46  QSSAPVEIPDWSKIYGKTSKKKKNGTISSSWVDYN---AVNHDGYYGNGGNDHHHHHLNG 105

Query: 140 SDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLKGRDLCKVRNSI 199
             +  DGD DD DDDDD+MVPPHEYLAK+LARS+ISSFSVYEGIGRTLKGRDL KVRN+I
Sbjct: 106 YYDVGDGD-DDGDDDDDDMVPPHEYLAKKLARSQISSFSVYEGIGRTLKGRDLSKVRNAI 165

Query: 200 LTKTGFLE 201
           LTKTGFLE
Sbjct: 166 LTKTGFLE 169

		  

BLAST of Spo20562.1 vs. UniProtKB/TrEMBL
Match: B9H111_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s01400g PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.700e-32
Identity = 94/199 (47.24%), Postives = 128/199 (64.32%), Query Frame = 1

		  

Query: 3   GRHGRKYVKDEEFEEEEVWGVMEENSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMIPR 62
           G +GR+ ++DE+FEEE+VW V++E           K  S+   F++     ++A RMIP 
Sbjct: 16  GGYGRRSMRDEDFEEEDVWSVVKEMGDSSSNS---KYPSSGPSFSSAWHLPSTAPRMIPA 75

Query: 63  SSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGA-SSWVDYNNAVVTTHDVYYGH 122
           + T++++      +  +QSSAP++IPDWSKIYGK +    +SWVD +N +          
Sbjct: 76  TKTTATN--HGGKAAQQQSSAPVNIPDWSKIYGKDSRDIMASWVDDHNGIA--------- 135

Query: 123 GCHGHVNHGDGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLK 182
                  +GDG D   D   D D+D DD +VPPHE+LA++LARS+ISSFSV EGIGRTLK
Sbjct: 136 -------YGDGDDHVNDDVDDCDEDGDDGIVPPHEWLARKLARSQISSFSVCEGIGRTLK 193

Query: 183 GRDLCKVRNSILTKTGFLE 201
           GRDL KVRN+ILTKTGFLE
Sbjct: 196 GRDLSKVRNAILTKTGFLE 193

		  

BLAST of Spo20562.1 vs. UniProtKB/TrEMBL
Match: B9RXK6_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0904330 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 8.500e-32
Identity = 101/214 (47.20%), Postives = 141/214 (65.89%), Query Frame = 1

		  

Query: 3   GRHGRKYVKDEEFEEEEVWGVMEE--NSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMI 62
           G + R+ ++DE+FEEE++W V++E  +SSPK+RK   K +  +   ++     +SA RMI
Sbjct: 14  GGYSRRSMRDEDFEEEDIWSVVKEREDSSPKIRKS--KDNYCSSSPSSSAWRLHSAPRMI 73

Query: 63  PRSSTSSSSLLSSPSSCG------KQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTT 122
           PR++  S +    P++        +QSSAP++IPDWSKIYGK N    SW + ++     
Sbjct: 74  PRANIVSPTSAGVPATSTHEAKAVQQSSAPVNIPDWSKIYGK-NTRMGSWANNDDDDDDD 133

Query: 123 HD-----VYYGHGCHGHVNHGDG---SDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSK 182
            D     VY     HGHVN G+G   +  + + D + +DD++D MVPPHE++AK+LARS+
Sbjct: 134 DDDDDGIVYDDGEDHGHVN-GNGYYYNYNNNNVDVNGNDDEEDEMVPPHEWIAKKLARSQ 193

Query: 183 ISSFSVYEGIGRTLKGRDLCKVRNSILTKTGFLE 201
           ISSFSV EGIGRTLKGRDL KVRN+IL KTGFLE
Sbjct: 194 ISSFSVCEGIGRTLKGRDLSKVRNAILAKTGFLE 223

		  

BLAST of Spo20562.1 vs. UniProtKB/TrEMBL
Match: I1KND2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G260700 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.600e-30
Identity = 93/196 (47.45%), Postives = 127/196 (64.80%), Query Frame = 1

		  

Query: 10  VKDEEFEEEEVWGVMEENS----SPKLRKLPLKSSSATKHFTAVTGGNNSAARMIPRS-S 69
           +++E+F+E +VWGV  E      SPKLR     S S++           ++ R IPR+ S
Sbjct: 19  MRNEDFDEGDVWGVAMERERNFLSPKLRVSKESSGSSSAWLLP------TSPRKIPRTNS 78

Query: 70  TSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYGHGCH 129
           T+ +SL S  S+  ++SSAPMDIPDWSKIYGK+              V+   +  G+G H
Sbjct: 79  TTPNSLESDSSNVVQRSSAPMDIPDWSKIYGKRGVEEG---------VSNKKLDCGYGDH 138

Query: 130 GHVNHGDGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLKGRD 189
               +GD  D+D+DG+      D+D+M+PPHE++A++LARS+ISSFSV EGIGRTLKGRD
Sbjct: 139 ----YGDDDDDDDDGE------DEDDMIPPHEWIARKLARSQISSFSVCEGIGRTLKGRD 189

Query: 190 LCKVRNSILTKTGFLE 201
           L KVRN+ILTKTGF+E
Sbjct: 199 LSKVRNAILTKTGFIE 189

		  

BLAST of Spo20562.1 vs. TAIR (Arabidopsis)
Match: AT4G04630.1 (Protein of unknown function, DUF584)

HSP 1 Score: 120.9 bits (302), Expect = 8.700e-28
Identity = 85/192 (44.27%), Postives = 115/192 (59.90%), Query Frame = 1

		  

Query: 12  DEEFEEEEVWGVMEEN--SSPKLRKLPLKSSSATKHFTAVTGGNNSAARMIPRSSTSSSS 71
           DEEF+EE+VW V+ E   SSP++ K+P        HF++ +  ++S   +  R S   S 
Sbjct: 24  DEEFQEEDVWSVLREGETSSPEM-KIP------KSHFSSSSSSSSSPWNI--RRSKEVSG 83

Query: 72  LLSSPSSCGKQSSAPMDIPDWSKIYG-KKNNGASSWVDYNNAVVTTHDVYYGHGCHGHVN 131
           +        KQSSAPM++PDWSK+YG  K+N  SS                    H H +
Sbjct: 84  V--------KQSSAPMNVPDWSKVYGDSKSNRRSS--------------------HLHSH 143

Query: 132 HGDGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLKGRDLCKV 191
             D          DDD+DDD  MVPPHE++A++LAR++ISSFS+ EG+GRTLKGRDL KV
Sbjct: 144 AAD----------DDDEDDDGCMVPPHEWVARKLARTQISSFSMCEGVGRTLKGRDLSKV 168

Query: 192 RNSILTKTGFLE 201
           RN++L+KTGFLE
Sbjct: 204 RNAVLSKTGFLE 168

		  

BLAST of Spo20562.1 vs. TAIR (Arabidopsis)
Match: AT4G21970.1 (Protein of unknown function, DUF584)

HSP 1 Score: 115.9 bits (289), Expect = 2.800e-26
Identity = 79/188 (42.02%), Postives = 105/188 (55.85%), Query Frame = 1

		  

Query: 13  EEFEEEEVWGVMEENSSPKLRKLPLKSSSATKHFTAVTGGNNSAARMIPRSSTSSSSLLS 72
           EEF+EEEVW V+ E+ +   +   +K S +   F+A    ++S+AR IP+ +  S     
Sbjct: 5   EEFQEEEVWSVLRESET---QGPEMKMSKSNNLFSA---SSSSSARYIPKGNEVSGG--- 64

Query: 73  SPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYGHGCHGHVNHGDG 132
                 KQSSAPM+IPDWSK+YG      SS +                       H   
Sbjct: 65  -----AKQSSAPMNIPDWSKVYGYSKKNTSSHL-----------------------HSWA 124

Query: 133 SDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLKGRDLCKVRNSI 192
            D+D++G            VPPHE +AKRLAR++ISSFS+ EGIGRTLKGRDL K RN++
Sbjct: 125 IDDDDEGSM----------VPPHELVAKRLARTQISSFSMCEGIGRTLKGRDLSKTRNAV 145

Query: 193 LTKTGFLE 201
           LT+TGFLE
Sbjct: 185 LTRTGFLE 145

		  

BLAST of Spo20562.1 vs. TAIR (Arabidopsis)
Match: AT3G45210.1 (Protein of unknown function, DUF584)

HSP 1 Score: 73.6 bits (179), Expect = 1.600e-13
Identity = 36/59 (61.02%), Postives = 49/59 (83.05%), Query Frame = 1

		  

Query: 142 DDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLKGRDLCKVRNSILTKTGFLE 201
           ++DDD  +  +PPHEYLAK    ++++SFSV+EGIGRTLKGRD+ +VRN+IL KTGFL+
Sbjct: 94  ENDDDGGEGKLPPHEYLAK----TRMASFSVHEGIGRTLKGRDMSRVRNAILEKTGFLD 148

		  

BLAST of Spo20562.1 vs. TAIR (Arabidopsis)
Match: AT5G03230.1 (Protein of unknown function, DUF584)

HSP 1 Score: 71.2 bits (173), Expect = 7.900e-13
Identity = 58/150 (38.67%), Postives = 72/150 (48.00%), Query Frame = 1

		  

Query: 62  RSSTSSSSLLSSPSSCG--------KQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVT 121
           + S S S L   P+  G        K  S P++IPDWSKI   +                
Sbjct: 50  KRSISISRLRRKPTKTGDSGNREITKTGSLPVNIPDWSKILKSE---------------- 109

Query: 122 THDVYYGHGCHGHVNHGDGSDEDEDGDKDDDDDDDDN-----MVPPHEYLAKRLARSKIS 181
               Y GH              D+D D DD+DDDD N     M+PPHEYLA+R      S
Sbjct: 110 ----YRGHAI-----------PDDDSDDDDEDDDDSNDGGRRMIPPHEYLARRRG----S 164

Query: 182 SFSVYEGIGRTLKGRDLCKVRNSILTKTGF 199
           SF+V+EGIG T KGRDL ++RN+I  K GF
Sbjct: 170 SFTVHEGIGGTAKGRDLRRLRNAIWEKIGF 164

		  

BLAST of Spo20562.1 vs. TAIR (Arabidopsis)
Match: AT5G60680.1 (Protein of unknown function, DUF584)

HSP 1 Score: 70.9 bits (172), Expect = 1.000e-12
Identity = 51/137 (37.23%), Postives = 72/137 (52.55%), Query Frame = 1

		  

Query: 62  RSSTSSSSLLSSPSSCGKQSSAPMDIPDWSKIYGKKNNGASSWVDYNNAVVTTHDVYYGH 121
           RS   SS+  S+ SS    SS P+++PDWSKI                            
Sbjct: 58  RSGKKSSNRPSAASSAAAASSLPVNVPDWSKIL--------------------------- 117

Query: 122 GCHGHVNHGDGSDEDEDGDKDDDDDDDDNMVPPHEYLAKRLARSKISSFSVYEGIGRTLK 181
              G          +++ D DDD++D  + +PPHE+LAK    ++++SFSV+EG+GRTLK
Sbjct: 118 --RGEYRDNRRRSIEDNDDDDDDNEDGGDWLPPHEFLAK----TRMASFSVHEGVGRTLK 161

Query: 182 GRDLCKVRNSILTKTGF 199
           GRDL +VRN+I  K GF
Sbjct: 178 GRDLSRVRNAIFEKFGF 161