Spo20149 (gene)

Overview
NameSpo20149
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionVesicle-associated protein 1-4 (Plant VAP homolog 14) (AtPVA14) (VAMP-associated protein 1-4)
LocationSuper_scaffold_170 : 427105 .. 432606 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGACAGAAATATAAACGAAGAAGAGAGAACAGCCGGTGGGTACAACGGCCTCCACTACCAAAACCCCCCCAAATACAATCACATCTTTCATTTTCTTACCACCAAACACACCTTTAAAACATCACTCCCTCCATTTTTACTTCCCTTTTACTTCTTCTTCCTCTGTTCTTCCCCAATTTTATCTCTCCTCTCTTTCGACACACCATTCCATAATCGTACTACTGCAACTTTTTTTTTTTATTATAGTATTGCGTTTCGAGTAATCTCAACGCCATTTCTACGAACTCCAATTTATTACGATCAATTCTTTAAATAGTAAGATCTCTTCCTCTTTCTCTCTTCCTATATTGTACAATTTTTTCTTGATTTTTTTTTTTTTTTGTGTAATTTTGTTGAAATTCGTGAATCTGATTCTGTTCTTTTGAGTTGATATTCCGAGTCAAATCGATCCTGTTTCAAAAAATTTGAAAATTTATCGTCGCTTTTCGAACATTCGGGTAAATGCCTTTTGGGCGCTTTATTATTGTATGATCGCGCCTTTTGTGATCTGGATTTTTGCTACTTTATTTTTCTTTTGAATAAATGTAATTATGCAATTACTTTTGAAGAACTTTTAGGGTTTAGTTGAATTAATTATCAATTTCCTTTTTACCCTAAATGATTCGACTGCTTTTTGTTTTTTGCAATATTATTGAAAAAGTTGAATTGTAATTGTGTTTTGGCTATTTTCGTTGAAATTGCAGGAAATTTAGCTGTGTATGCTGAGATAAGCAATCATCATAAGAAAAAAATCATGAGTTCGCAGTTGGAGATTCAACCTCGTGAGCTTAAATTCGTTGGTTAGTCGCTAATCTTTTAATTATAATCAATTACCACTTATTTTGCAATTATTAGCAAATGATTGAATTTCCTGTTGATTAGTTGGATTTACAATAACTTAGACTATCTTTATTTTTTGTGGGGTGAACTAATATGGTTCTGTTGTAATTTATGTTTATTTGTTTGGGGTGCTAGTAGTTGAATTGAACAAACAAAGCAAGTGTACGGTTCGTCTCAGCAACAAAACTGATCAATATGTTGCTTTCAAGGTACAATTTGACTGTCTTTTGGTTTGAATTCGATCTTAAGGTTATACCGTTCTTCAGTTTCCTGCTTGAATATATTGATTTCAGGAAATAAGTTTTGGTATGAACATGATTGTTGCAGGTTAAGACTACATCGCCGAAGAAATATTGTGTGCGGCCTAACGTTGGTGTCATTCAGCCGAAATCAATTCATGAATTTGTTGGTATGCCATTGTGTAACTTGTGTTTTTTTGAGAAAAGAGATTATATTATGTTATCAGTAGGAAAAAAGGAGAGTAAACAAAGAGGGCAAGCTGTCCTCTGATCAAGAAACTTGTTAGTTATCACTACTTCAGTCTCAAAATATTAGGTTGAATCTAACCTCAATGATCATAATTGATTAACTTTGACTGTTTATTTATCAAAAATTTATTGAGTTAGAGTGTGTAAGTAACAGTTTTCTAAAAAACAATACTGAGTACCTTTTTAAATTGATATTTTGCAAATTTACTGACTGGTATACGGATAGAAATAATCTCAACAAAATAAAATTTGTCCATGAAAGTTGTTGTAGCCTATTATTTTCAGTAGGAGTATGTTTTTCTTGCCTGGTAAAAGTTTAGCATAGGTGAGCTTTCCAAATTACTAAGATAACAGGAGTTGAGAGGTAGGATGTTGATCATTAAGAACTAAGTTGTGGAGATTGCCCCTCAGCAGTGACTCGTGGGGATTTGACGCGTATACTTATATTCATCATTATCATTACCCCATTGCCTCAAAGAGGCTCCCGCAAGAAGCGGGGTAAGGGAGGGTCGGACGTACGCAACCTTACCCCTGCAATTGCAGGGAGGTTGTTTCAATTGACCCAAAAGCGACAACAGGACGACCATCTTCTACTTCATGGAAAGGAAGCGTATACATATATTAGTTTTAAATTTTAAGTGGTTAAAGTATTTACTATCTCTGTTTTTTTAGCTGTACTAGTTTGACTTTGCACTATTCAGTATTAACATACCTCACTCTGACCAAATTATTAGTGAAGTACCAATATATATGTAACAAAATGATCATGTAATTTTTAGTAATAGATAGTTAAAGATATTAAGGGTCAACAAAAATAGAAGTAGGTGAAAACTATATGCACCAAATAGACAGGTTAACACTCTAAATTGAGTCCACTGGAAATTTTGAGAAGCAATTGAACTATTGAAGTCTTCAAAAATTGGTGGGTTTAGTAAGGGCACTCTGGTGTGTTGCGTGATGATATTTTACATTGGTCAACCTAACAGGTAATTTTAAGTAAATTGTTGCAGAGGTACATTTTCTGATTTGACTTCGAAGTTCCGAGTAAAATTTATAGTGCTTTGTTATAGCCTTTGAGAAATGAGGACGGTTTCATGTGAATATTCTTTTGCATATTTGGTTATTTGACTTATTTCCTTTTGCTAGTTGCTTCTCTAAAATTTTATTGATCAAATGTTGACTTTTTAGATTTATAAAAACAATGGGAGGTATATCCAAAGCCTACTTTCCAGAAGACTTGTGGTCCTTCATATCTTAGCACAGCTATCTCCTAAGCTTCTGTTTTCCTTGGTGCCATTGCATACCTGATTTCTTGTTACCTCACTAGAATATTTCAGTAATTTGTTTGCCATTAAGGGTCAAATGTAGCTTTTGAAGTGAGGTCTTTAGGAAACGTTGAAGAATTATGGGAGAAGTTCTCAATGGGTAAGACATAGGTGAAATATTAATTATTTCTAGCAAAATCTAAATGTTCCTAAAACATACCTTCTGGCCTTTAGTGATGATCGACCCATATGGCTTATGCATTTTCACTGATTAAGAAGCCAGTTTTTGTACGGAGTACATTTGGTGTAGGTGAAGGCATTTATCTCTTTTTTTATTCCTGAAGTATACAACTGGTGTTAAAGATCTGCAATGTGCCATTGGTAACTAAAGACTGGCTGGTTCCGCCTCCTGCTTTCCAGAGTTTAGCTCTTGAGATTTGATTGAATAGATTATTGGATGAGTCAGATAGTGCATCTACTTATCAGAAAACAAGTACCATTAGTCTAGCTTTTGTTAATAATCTTCCTGAACCAGTATTTTCTTTTTGCTACATACCTCTGTTTAATTCTGATGAATAATCCTAGTTGCTGCAATCAGTACCCACTACCCAGTCTCATTTTATTCTTTTGTTGCTGCAGTCATTATGCAAGCTCTGAAAACAGCTCCTGTTGAAACAACATGCAAAGACAAGTTCCTGGTTCAAAGCACAATTGTCCCTGAAGGAACAGTGGAAGAGGACATTACATCTGAAATGGTACGAGACAGTTGTTGATTTTGAAAATTTTGAAGTTCACATATGCTGATATGCTTATTGTTTTCCTTGCAGTTCTCTAAGGATGGTGTCAGATACGTGGAAGAGATAAAAATGAGAGTGAACCTTGTTGTCCCACCCCCTAAATCTCCAGAGTTGTTACCCCTCAACGGAACGCTGAAGCAGGTAAAAGCTTATGATATTTGAGTTGCAGATACCTATTATGCTATGGAGCAAAGACTCCCAATCACCATTGTATGGAGCAAAGTAGCATATCATTGGATCTTGAATCCTCTTATGCAGCCCTGTTCTGCTTTAACACTTAACTCGTCTCATGCTGGGTTTTATATATATATATATATATATATATATATATATACAGAACCTCATAAACTTTACCTATTCCTTTTATTTTACTACAATGCATAAAAAAATATTATAATGTGGGATCTTTTTGGATTTATTTGAATGTGTGTATTGAAAGTATCAATTTTTCATAATATTTATCGATGGTTAATTAAAGATAATAATGGGAAAAGCTTTGCGCTGGAGGTTGTAAAAACTAAAAAGGCTAGTGTTGCAATAAAAAGAAATGGGGAAGTACTCCATATTAGTTTAGGAGTTTCCACATCTTTAGGCTTCATCCATTATGTTTCTGATTACATTTACTTTTTGTTGCTGTATTATGCTCCCTGATTACCAGGAAAAGAGTATCTTTCTTTTCCGGAACTTTCAAGATGTTTGGTAAGTTGTTGATATTTATTTATATATGAATATTTTTAATGCTGCAGGCTGATGAGCATGTCGAGTCAAAAACAGTGAATAAGGAGGAGTTGAAGCCAGTGAAGGATGCGGATGTAGCTCGACCTCAGGATATGAATTTAGAACCAATTAAGCAGATGAAATTTGATCTAAGCAAGGCTGTTGACTCAGCTGTGGAAAAAGATGTGGAGTCAGAACTCACTAAGGAAATAGAACCAATAATAAGAAACAATGAGGAACCAAAAGTAATTGCATCGAGAACCTCAATGAATGAGGAACAAAGATTGTCCAAGGATGTCAATGAGATGAAATCAAAACTGCGTGAACTTGAGTTGAAATTAAGCAAGGTCAGTTGTACTTAAGTTTATGTCAATAGCTACAACGCTATGCTGCTACTCCTGTTCTTCCCCTTTGTTTCTTCCACATCAACTCACTAATCCTCGGTGGTTGTTCTCTCTTATTATTTCCTTAAGCATGTTCTTGTACTTTGACTTCTTTTAGAGAGCTGAATACTTTAGTAAGAACAATACTCTCTCGTGGTTTTGCTGAAACTCGGATTTGAAACTTGTGATGCTTATTGCTAAATGATAGCCTGCTGCAGTTTTCTGTAGGTTTTGTTCCTCAGCCTTTTACTAGGAAGACAATAGTTGGGAACTTGGGATGTGTGTGAGGCTTAGAATAATTTACATCATTATTATGTTGTACATCTGCTCTCCTAAAATCTATCTGCCCTCTGAATGGTGTGGTTAATAAAGAAACTTATTGAGAGTGTAATATGCAAAGTAATGGGGGAAATATCACATGTAAAACTACCCTGGGATTTGCTTTTCTGTGTTTTGTTCTGAACAGAATGCATATCATCTGACATGACCTCATTTACCTGGGCAGCTATTCCTGTGTTATCTGGCTATTCCTGTGTTATCTGGCCTTCCTAATAACTAAAAAATGTCTTGTGGATTTTGCAGGCAGAAGTGAATGTTTCAAATCTGACAGAAGAGAGGAGGCTGGTCATTCAAGAGAGGGAGAATTTGAAACAAGAAATGGTATTCCCTGTATTCTAAATTGATTATCAGTATAAGCCATATTTAACCTTCATTTTTGGTGTTTGCTAAAGGTAGAGCATATTGAACGATCTCCTTTTTCTGAAATTGTCTGCAGGCTTTTCTAAGATTGAAAAAAGTAGAAAGAAGAGTGCAGGATGGATTTCCGCTTCTGTTTGTCTGCATGGTAGCACTAATCAGTCTTATTGCTGGGTATGGCTTTCATCCCTGAGAAAACTAGTGTTTGAGGCCTGAGCATGAACCACATTCTTAGCCTCTTCCTGTCTTCTTCTAATACAAAAAGAATAGATGA

mRNA sequence

AAAAGACAGAAATATAAACGAAGAAGAGAGAACAGCCGGTGGGTACAACGGCCTCCACTACCAAAACCCCCCCAAATACAATCACATCTTTCATTTTCTTACCACCAAACACACCTTTAAAACATCACTCCCTCCATTTTTACTTCCCTTTTACTTCTTCTTCCTCTGTTCTTCCCCAATTTTATCTCTCCTCTCTTTCGACACACCATTCCATAATCGTACTACTGCAACTTTTTTTTTTTATTATAGTATTGCGTTTCGAGTAATCTCAACGCCATTTCTACGAACTCCAATTTATTACGATCAATTCTTTAAATAGAAATTTAGCTGTGTATGCTGAGATAAGCAATCATCATAAGAAAAAAATCATGAGTTCGCAGTTGGAGATTCAACCTCGTGAGCTTAAATTCGTTGTTGAATTGAACAAACAAAGCAAGTGTACGGTTCGTCTCAGCAACAAAACTGATCAATATGTTGCTTTCAAGGTTAAGACTACATCGCCGAAGAAATATTGTGTGCGGCCTAACGTTGGTGTCATTCAGCCGAAATCAATTCATGAATTTGTTGTCATTATGCAAGCTCTGAAAACAGCTCCTGTTGAAACAACATGCAAAGACAAGTTCCTGGTTCAAAGCACAATTGTCCCTGAAGGAACAGTGGAAGAGGACATTACATCTGAAATGTTCTCTAAGGATGGTGTCAGATACGTGGAAGAGATAAAAATGAGAGTGAACCTTGTTGTCCCACCCCCTAAATCTCCAGAGTTGTTACCCCTCAACGGAACGCTGAAGCAGGCTGATGAGCATGTCGAGTCAAAAACAGTGAATAAGGAGGAGTTGAAGCCAGTGAAGGATGCGGATGTAGCTCGACCTCAGGATATGAATTTAGAACCAATTAAGCAGATGAAATTTGATCTAAGCAAGGCTGTTGACTCAGCTGTGGAAAAAGATGTGGAGTCAGAACTCACTAAGGAAATAGAACCAATAATAAGAAACAATGAGGAACCAAAAGTAATTGCATCGAGAACCTCAATGAATGAGGAACAAAGATTGTCCAAGGATGTCAATGAGATGAAATCAAAACTGCGTGAACTTGAGTTGAAATTAAGCAAGGCAGAAGTGAATGTTTCAAATCTGACAGAAGAGAGGAGGCTGGTCATTCAAGAGAGGGAGAATTTGAAACAAGAAATGGCTTTTCTAAGATTGAAAAAAGTAGAAAGAAGAGTGCAGGATGGATTTCCGCTTCTGTTTGTCTGCATGGTAGCACTAATCAGTCTTATTGCTGGATGA

Coding sequence (CDS)

ATGAGTTCGCAGTTGGAGATTCAACCTCGTGAGCTTAAATTCGTTGTTGAATTGAACAAACAAAGCAAGTGTACGGTTCGTCTCAGCAACAAAACTGATCAATATGTTGCTTTCAAGGTTAAGACTACATCGCCGAAGAAATATTGTGTGCGGCCTAACGTTGGTGTCATTCAGCCGAAATCAATTCATGAATTTGTTGTCATTATGCAAGCTCTGAAAACAGCTCCTGTTGAAACAACATGCAAAGACAAGTTCCTGGTTCAAAGCACAATTGTCCCTGAAGGAACAGTGGAAGAGGACATTACATCTGAAATGTTCTCTAAGGATGGTGTCAGATACGTGGAAGAGATAAAAATGAGAGTGAACCTTGTTGTCCCACCCCCTAAATCTCCAGAGTTGTTACCCCTCAACGGAACGCTGAAGCAGGCTGATGAGCATGTCGAGTCAAAAACAGTGAATAAGGAGGAGTTGAAGCCAGTGAAGGATGCGGATGTAGCTCGACCTCAGGATATGAATTTAGAACCAATTAAGCAGATGAAATTTGATCTAAGCAAGGCTGTTGACTCAGCTGTGGAAAAAGATGTGGAGTCAGAACTCACTAAGGAAATAGAACCAATAATAAGAAACAATGAGGAACCAAAAGTAATTGCATCGAGAACCTCAATGAATGAGGAACAAAGATTGTCCAAGGATGTCAATGAGATGAAATCAAAACTGCGTGAACTTGAGTTGAAATTAAGCAAGGCAGAAGTGAATGTTTCAAATCTGACAGAAGAGAGGAGGCTGGTCATTCAAGAGAGGGAGAATTTGAAACAAGAAATGGCTTTTCTAAGATTGAAAAAAGTAGAAAGAAGAGTGCAGGATGGATTTCCGCTTCTGTTTGTCTGCATGGTAGCACTAATCAGTCTTATTGCTGGATGA

Protein sequence

MSSQLEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMKFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKLRELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVALISLIAG
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo20149.1Spo20149.1mRNA


Homology
[Close]
BLAST of Spo20149.1 vs. NCBI nr
Match: gi|902167614|gb|KNA07399.1| (hypothetical protein SOVF_172200 [Spinacia oleracea])

HSP 1 Score: 563.5 bits (1451), Expect = 2.200e-157
Identity = 306/307 (99.67%), Postives = 306/307 (99.67%), Query Frame = 1 

		  

Query: 1   MSSQLEIQPRELKF-VVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQP 60
           MSSQLEIQPRELKF VVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQP
Sbjct: 1   MSSQLEIQPRELKFVVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQP 60

Query: 61  KSIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKM 120
           KSIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKM
Sbjct: 61  KSIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKM 120

Query: 121 RVNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQM 180
           RVNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQM
Sbjct: 121 RVNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQM 180

Query: 181 KFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKL 240
           KFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKL
Sbjct: 181 KFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKL 240

Query: 241 RELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVA 300
           RELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVA
Sbjct: 241 RELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVA 300

Query: 301 LISLIAG 307
           LISLIAG
Sbjct: 301 LISLIAG 307
[Close]
BLAST of Spo20149.1 vs. NCBI nr
Match: gi|731312073|ref|XP_010672484.1| (PREDICTED: vesicle-associated protein 2-2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 460.7 bits (1184), Expect = 2.000e-126
Identity = 249/307 (81.11%), Postives = 277/307 (90.23%), Query Frame = 1 

		  

Query: 1   MSSQLEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPK 60
           MSSQLEIQPRELKF VEL+KQSKCTVRL N+TDQY+AFKVKTTSPKKYCVRPNVGVIQPK
Sbjct: 1   MSSQLEIQPRELKFAVELDKQSKCTVRLVNRTDQYLAFKVKTTSPKKYCVRPNVGVIQPK 60

Query: 61  SIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMR 120
           S+HEF+VIMQAL+TAP +TTCKDKFLVQSTIV EGTVEEDITS+MF KDGVRYVEEIKMR
Sbjct: 61  SVHEFIVIMQALRTAPADTTCKDKFLVQSTIVQEGTVEEDITSDMFVKDGVRYVEEIKMR 120

Query: 121 VNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMK 180
           VNLV+PPP+SPELLP++GTLKQADE+  SK VN E+LKPVKD D+A+ QD N+EPIK+MK
Sbjct: 121 VNLVLPPPQSPELLPIDGTLKQADENGVSKAVNNEDLKPVKDVDIAQLQD-NVEPIKKMK 180

Query: 181 FDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMN-EEQRLSKDVNEMKSKL 240
           F  SK VDS + KDVES+LTKEIEP+IRN+ E KVI SR  +  E+QRL KDV EMKSKL
Sbjct: 181 FGSSKVVDSIIPKDVESQLTKEIEPVIRNDVESKVIESRIPVRAEDQRLFKDVEEMKSKL 240

Query: 241 RELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVA 300
            ELELKLSKAEV +SNLTEERR+VIQERE+LKQEM FLR +KVERRVQDGFPLLFVCMVA
Sbjct: 241 HELELKLSKAEVTISNLTEERRVVIQERESLKQEMGFLRTRKVERRVQDGFPLLFVCMVA 300

Query: 301 LISLIAG 307
           LISLIAG
Sbjct: 301 LISLIAG 306
[Close]
BLAST of Spo20149.1 vs. NCBI nr
Match: gi|661877819|emb|CDP18378.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 244.2 bits (622), Expect = 3.000e-61
Identity = 151/302 (50.00%), Postives = 196/302 (64.90%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           L+IQPRELKF+VEL KQS C+VRL NK+  YVAFKVKTTSPKKYCVRPN GVI+PKS  +
Sbjct: 6   LDIQPRELKFIVELKKQSSCSVRLFNKSKHYVAFKVKTTSPKKYCVRPNTGVIEPKSACD 65

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
           F+V MQA K A  + TCKDKFLVQ T+V EGT ++DITS +F+K+  +YVEE K+RV ++
Sbjct: 66  FLVTMQAQKVALPDMTCKDKFLVQGTVVAEGTTDDDITSSLFAKENGKYVEESKLRV-VL 125

Query: 125 VPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMKFDLS 184
             PP SP LLP+NG   Q         V   E  P KD  +   +++  +          
Sbjct: 126 ASPPHSPVLLPINGVHDQ---------VPVFETSPSKDQVLRGDENVGTQ---------L 185

Query: 185 KAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKLRELEL 244
           K +++ +E+  E+     IEP +           R    EE  L+KDV  +K K+ ELEL
Sbjct: 186 KVIENMMEQKSEN-----IEPPVTVEARD---LERVKGVEELELAKDVENLKFKISELEL 245

Query: 245 KLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVALISLI 304
           KL +A+V +S LTEERRL  +E E+L+QE+A L  KK  RRVQ GFP LFVCMV+L+S++
Sbjct: 246 KLEEAQVTISELTEERRLTARESESLRQELALLSTKKGVRRVQVGFPFLFVCMVSLVSVV 280

Query: 305 AG 307
            G
Sbjct: 306 LG 280
[Close]
BLAST of Spo20149.1 vs. NCBI nr
Match: gi|1021052858|gb|KZN10636.1| (hypothetical protein DCAR_003292 [Daucus carota subsp. sativus])

HSP 1 Score: 240.4 bits (612), Expect = 4.300e-60
Identity = 153/347 (44.09%), Postives = 211/347 (60.81%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           L+IQP+ELKF+ EL KQS C+VRL NKT+Q+VAFKVKTTSPKKYCVRPN G+++PK I +
Sbjct: 6   LDIQPQELKFIFELKKQSSCSVRLVNKTNQHVAFKVKTTSPKKYCVRPNTGIVKPKEICD 65

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
           F V MQA +  P E  CKDKFL+Q  +VP G  EEDITS  F+KDG RY+EE K+RV ++
Sbjct: 66  FTVTMQAQRVVPPEMICKDKFLLQCAVVPVGMAEEDITSATFAKDG-RYIEEKKLRV-IL 125

Query: 125 VPPPKSPELLPLNGTLKQA------------------------DEHVESKTVNKEELKPV 184
           V PP SP   P+NGT KQ                         D+  E+K V  E+LKPV
Sbjct: 126 VSPPNSPIHSPVNGTAKQVYDVPTPEEQHPNYTGNFSPPQTGTDDLRETKVVASEDLKPV 185

Query: 185 K------------DA--DVARPQDMNLEPIKQMKFDL------SKAVDSAVEKDVESELT 244
           +            DA  D+   +    E +K +K +       S+  +  ++  V S+  
Sbjct: 186 RHEAAEELNPVKHDAAEDLKPVKHETAEDLKPVKHEADEELIRSRDFEQGIKNHVVSKTV 245

Query: 245 KEIE-PIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKLRELELKLSKAEVNVSNLTEE 304
           K++E  +++  EE K   +     +E +L KD+ E+K KLRE E KL++AE  +S LTEE
Sbjct: 246 KDVELKVVKEFEEQKPPTNMMKDGDELKLVKDIEEVKFKLREFESKLNEAERTISKLTEE 305

Query: 305 RRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVALISLIAG 307
           +R+   +RE L+QE+  +R ++  ++ Q GFPLLFVCMVAL+S+  G
Sbjct: 306 KRVSTHDREILQQELGLMRSRRSGQKAQSGFPLLFVCMVALVSVWLG 350
[Close]
BLAST of Spo20149.1 vs. NCBI nr
Match: gi|225432396|ref|XP_002277190.1| (PREDICTED: vesicle-associated protein 1-2 [Vitis vinifera])

HSP 1 Score: 233.8 bits (595), Expect = 4.000e-58
Identity = 142/302 (47.02%), Postives = 196/302 (64.90%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           LEIQPRELKF  EL KQS C++ L NK+DQY+AFKVKTTSPK+YCVRPN GV++P +  +
Sbjct: 4   LEIQPRELKFTFELKKQSSCSIHLVNKSDQYLAFKVKTTSPKRYCVRPNTGVVKPNAECD 63

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
            +V MQA + AP +  CKDKFL+QST+VP GT E DI S+MF+KD  +Y+EE K+RV ++
Sbjct: 64  VMVTMQAQRVAPPDMQCKDKFLIQSTVVPYGTTETDIRSDMFAKDSGKYLEEKKLRV-VL 123

Query: 125 VPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMKFDLS 184
           + P  SP LLPLNG LKQ   H E+ +   + L  V++   A     ++E  K  K    
Sbjct: 124 IGPAHSPVLLPLNGLLKQDPCH-ENFSQKDQLLSGVENTPPAHVVAKDVEGFKTAKV--- 183

Query: 185 KAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKLRELEL 244
              +S   KDVES   ++++ +   N+  K           + L+KD+ E+ SKL  +EL
Sbjct: 184 -TDESRAAKDVESRPAEDVDELKLTNDIAK-----------ENLAKDIEELNSKLNVVEL 243

Query: 245 KLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVALISLI 304
            LS+A++ ++ LTEER +  +E+E LK E+A LR K   RRV+ GFP LFV ++ALI + 
Sbjct: 244 NLSEAKLIITKLTEERSMATREKETLKHELAMLRKKSDVRRVRVGFPFLFVLIIALIGIA 288

Query: 305 AG 307
           AG
Sbjct: 304 AG 288
[Close]
BLAST of Spo20149.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QJH0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_172200 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 1.500e-157
Identity = 306/307 (99.67%), Postives = 306/307 (99.67%), Query Frame = 1 

		  

Query: 1   MSSQLEIQPRELKF-VVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQP 60
           MSSQLEIQPRELKF VVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQP
Sbjct: 1   MSSQLEIQPRELKFVVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQP 60

Query: 61  KSIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKM 120
           KSIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKM
Sbjct: 61  KSIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKM 120

Query: 121 RVNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQM 180
           RVNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQM
Sbjct: 121 RVNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQM 180

Query: 181 KFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKL 240
           KFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKL
Sbjct: 181 KFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKL 240

Query: 241 RELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVA 300
           RELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVA
Sbjct: 241 RELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVA 300

Query: 301 LISLIAG 307
           LISLIAG
Sbjct: 301 LISLIAG 307
[Close]
BLAST of Spo20149.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D3D3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g006460 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.400e-126
Identity = 249/307 (81.11%), Postives = 277/307 (90.23%), Query Frame = 1 

		  

Query: 1   MSSQLEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPK 60
           MSSQLEIQPRELKF VEL+KQSKCTVRL N+TDQY+AFKVKTTSPKKYCVRPNVGVIQPK
Sbjct: 1   MSSQLEIQPRELKFAVELDKQSKCTVRLVNRTDQYLAFKVKTTSPKKYCVRPNVGVIQPK 60

Query: 61  SIHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMR 120
           S+HEF+VIMQAL+TAP +TTCKDKFLVQSTIV EGTVEEDITS+MF KDGVRYVEEIKMR
Sbjct: 61  SVHEFIVIMQALRTAPADTTCKDKFLVQSTIVQEGTVEEDITSDMFVKDGVRYVEEIKMR 120

Query: 121 VNLVVPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMK 180
           VNLV+PPP+SPELLP++GTLKQADE+  SK VN E+LKPVKD D+A+ QD N+EPIK+MK
Sbjct: 121 VNLVLPPPQSPELLPIDGTLKQADENGVSKAVNNEDLKPVKDVDIAQLQD-NVEPIKKMK 180

Query: 181 FDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMN-EEQRLSKDVNEMKSKL 240
           F  SK VDS + KDVES+LTKEIEP+IRN+ E KVI SR  +  E+QRL KDV EMKSKL
Sbjct: 181 FGSSKVVDSIIPKDVESQLTKEIEPVIRNDVESKVIESRIPVRAEDQRLFKDVEEMKSKL 240

Query: 241 RELELKLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVA 300
            ELELKLSKAEV +SNLTEERR+VIQERE+LKQEM FLR +KVERRVQDGFPLLFVCMVA
Sbjct: 241 HELELKLSKAEVTISNLTEERRVVIQERESLKQEMGFLRTRKVERRVQDGFPLLFVCMVA 300

Query: 301 LISLIAG 307
           LISLIAG
Sbjct: 301 LISLIAG 306
[Close]
BLAST of Spo20149.1 vs. UniProtKB/TrEMBL
Match: A0A068VFA2_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00012958001 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.100e-61
Identity = 151/302 (50.00%), Postives = 196/302 (64.90%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           L+IQPRELKF+VEL KQS C+VRL NK+  YVAFKVKTTSPKKYCVRPN GVI+PKS  +
Sbjct: 6   LDIQPRELKFIVELKKQSSCSVRLFNKSKHYVAFKVKTTSPKKYCVRPNTGVIEPKSACD 65

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
           F+V MQA K A  + TCKDKFLVQ T+V EGT ++DITS +F+K+  +YVEE K+RV ++
Sbjct: 66  FLVTMQAQKVALPDMTCKDKFLVQGTVVAEGTTDDDITSSLFAKENGKYVEESKLRV-VL 125

Query: 125 VPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMKFDLS 184
             PP SP LLP+NG   Q         V   E  P KD  +   +++  +          
Sbjct: 126 ASPPHSPVLLPINGVHDQ---------VPVFETSPSKDQVLRGDENVGTQ---------L 185

Query: 185 KAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKLRELEL 244
           K +++ +E+  E+     IEP +           R    EE  L+KDV  +K K+ ELEL
Sbjct: 186 KVIENMMEQKSEN-----IEPPVTVEARD---LERVKGVEELELAKDVENLKFKISELEL 245

Query: 245 KLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVALISLI 304
           KL +A+V +S LTEERRL  +E E+L+QE+A L  KK  RRVQ GFP LFVCMV+L+S++
Sbjct: 246 KLEEAQVTISELTEERRLTARESESLRQELALLSTKKGVRRVQVGFPFLFVCMVSLVSVV 280

Query: 305 AG 307
            G
Sbjct: 306 LG 280
[Close]
BLAST of Spo20149.1 vs. UniProtKB/TrEMBL
Match: A0A162ALM6_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_003292 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.000e-60
Identity = 153/347 (44.09%), Postives = 211/347 (60.81%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           L+IQP+ELKF+ EL KQS C+VRL NKT+Q+VAFKVKTTSPKKYCVRPN G+++PK I +
Sbjct: 6   LDIQPQELKFIFELKKQSSCSVRLVNKTNQHVAFKVKTTSPKKYCVRPNTGIVKPKEICD 65

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
           F V MQA +  P E  CKDKFL+Q  +VP G  EEDITS  F+KDG RY+EE K+RV ++
Sbjct: 66  FTVTMQAQRVVPPEMICKDKFLLQCAVVPVGMAEEDITSATFAKDG-RYIEEKKLRV-IL 125

Query: 125 VPPPKSPELLPLNGTLKQA------------------------DEHVESKTVNKEELKPV 184
           V PP SP   P+NGT KQ                         D+  E+K V  E+LKPV
Sbjct: 126 VSPPNSPIHSPVNGTAKQVYDVPTPEEQHPNYTGNFSPPQTGTDDLRETKVVASEDLKPV 185

Query: 185 K------------DA--DVARPQDMNLEPIKQMKFDL------SKAVDSAVEKDVESELT 244
           +            DA  D+   +    E +K +K +       S+  +  ++  V S+  
Sbjct: 186 RHEAAEELNPVKHDAAEDLKPVKHETAEDLKPVKHEADEELIRSRDFEQGIKNHVVSKTV 245

Query: 245 KEIE-PIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKLRELELKLSKAEVNVSNLTEE 304
           K++E  +++  EE K   +     +E +L KD+ E+K KLRE E KL++AE  +S LTEE
Sbjct: 246 KDVELKVVKEFEEQKPPTNMMKDGDELKLVKDIEEVKFKLREFESKLNEAERTISKLTEE 305

Query: 305 RRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVALISLIAG 307
           +R+   +RE L+QE+  +R ++  ++ Q GFPLLFVCMVAL+S+  G
Sbjct: 306 KRVSTHDREILQQELGLMRSRRSGQKAQSGFPLLFVCMVALVSVWLG 350
[Close]
BLAST of Spo20149.1 vs. UniProtKB/TrEMBL
Match: D7T6K0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01970 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.800e-58
Identity = 142/302 (47.02%), Postives = 196/302 (64.90%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           LEIQPRELKF  EL KQS C++ L NK+DQY+AFKVKTTSPK+YCVRPN GV++P +  +
Sbjct: 4   LEIQPRELKFTFELKKQSSCSIHLVNKSDQYLAFKVKTTSPKRYCVRPNTGVVKPNAECD 63

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
            +V MQA + AP +  CKDKFL+QST+VP GT E DI S+MF+KD  +Y+EE K+RV ++
Sbjct: 64  VMVTMQAQRVAPPDMQCKDKFLIQSTVVPYGTTETDIRSDMFAKDSGKYLEEKKLRV-VL 123

Query: 125 VPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMKFDLS 184
           + P  SP LLPLNG LKQ   H E+ +   + L  V++   A     ++E  K  K    
Sbjct: 124 IGPAHSPVLLPLNGLLKQDPCH-ENFSQKDQLLSGVENTPPAHVVAKDVEGFKTAKV--- 183

Query: 185 KAVDSAVEKDVESELTKEIEPIIRNNEEPKVIASRTSMNEEQRLSKDVNEMKSKLRELEL 244
              +S   KDVES   ++++ +   N+  K           + L+KD+ E+ SKL  +EL
Sbjct: 184 -TDESRAAKDVESRPAEDVDELKLTNDIAK-----------ENLAKDIEELNSKLNVVEL 243

Query: 245 KLSKAEVNVSNLTEERRLVIQERENLKQEMAFLRLKKVERRVQDGFPLLFVCMVALISLI 304
            LS+A++ ++ LTEER +  +E+E LK E+A LR K   RRV+ GFP LFV ++ALI + 
Sbjct: 244 NLSEAKLIITKLTEERSMATREKETLKHELAMLRKKSDVRRVRVGFPFLFVLIIALIGIA 288

Query: 305 AG 307
           AG
Sbjct: 304 AG 288
[Close]
BLAST of Spo20149.1 vs. ExPASy Swiss-Prot
Match: VAP22_ARATH (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana GN=PVA22 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 9.300e-39
Identity = 99/191 (51.83%), Postives = 124/191 (64.92%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           L+IQPR L+F V+L KQ+ C V+L+N T  YVAFKVKTTSPKKYCVRPNVGV+ PKS  E
Sbjct: 6   LDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAPKSTCE 65

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
           F VIMQA K  P +  CKDKFL+QST V   T +EDIT+ MFSK   +++EE K+RV L 
Sbjct: 66  FTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKLRVTL- 125

Query: 125 VPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMKF--- 184
           VPP  SPEL P+N T KQ    V   ++ K+ L     ++   P     E +K+ +    
Sbjct: 126 VPPSDSPELSPIN-TPKQG--AVFEDSILKDRL--YSQSETLAPPQYEGEIVKEPRMVGH 185

Query: 185 DLSKAVDSAVE 193
           D  KA D+A E
Sbjct: 186 DELKAADNAKE 190
[Close]
BLAST of Spo20149.1 vs. ExPASy Swiss-Prot
Match: VAP11_ARATH (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana GN=PVA11 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.400e-31
Identity = 71/131 (54.20%), Postives = 89/131 (67.94%), Query Frame = 1 

		  

Query: 2   SSQLEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKS 61
           S  L ++P +L+F  EL KQ  C++ L+NKTD  VAFKVKTT+PKKYCVRPN GV+ P+S
Sbjct: 20  SELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRS 79

Query: 62  IHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRV 121
             E +V MQA K AP +  CKDKFL+Q  I   G   +++T EMFSK+    VEE K+RV
Sbjct: 80  TCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRV 139

Query: 122 NLVVPP-PKSP 132
             V PP P SP
Sbjct: 140 TYVAPPRPPSP 150
[Close]
BLAST of Spo20149.1 vs. ExPASy Swiss-Prot
Match: VAP12_ARATH (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana GN=PVA12 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.700e-30
Identity = 88/219 (40.18%), Postives = 122/219 (55.71%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           L I P +L+F  EL KQ  C++ L NKTD YVAFKVKTT+PKKYCVRPN GV+ P+S  E
Sbjct: 6   LTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPRSSSE 65

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
            +V MQA K AP +  CKDKFL+Q  +   G   +D+T EMFSK+    VEE K+RV  V
Sbjct: 66  VLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKLRVVYV 125

Query: 125 VPP-PKSPELLPLNGTLKQADEHVESKTVNKEE--------LKPVKDADVARPQDMNLEP 184
            PP P SP        +++  E   S   +  +          P   AD    QD N   
Sbjct: 126 APPRPPSP--------VREGSEEGSSPRASVSDNGNASDFTAAPRFSADRVDAQD-NSSE 185

Query: 185 IKQMKFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPK 215
            + +   L++  +SAV+  + + L +E++ + R ++  K
Sbjct: 186 ARALVTKLTEEKNSAVQ--LNNRLQQELDQLRRESKRSK 213
[Close]
BLAST of Spo20149.1 vs. ExPASy Swiss-Prot
Match: VAP14_ARATH (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana GN=PVA14 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.600e-30
Identity = 64/122 (52.46%), Postives = 89/122 (72.95%), Query Frame = 1 

		  

Query: 11  ELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHEFVVIMQ 70
           +++F +ELNKQ  C++ L+NKTD YVAFK +TT PK YCV+P+VGV+ P+S  E +V+MQ
Sbjct: 13  DIRFPIELNKQGSCSLNLTNKTDNYVAFKAQTTKPKMYCVKPSVGVVLPRSSCEVLVVMQ 72

Query: 71  ALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLVVPP-PK 130
           ALK AP +  CKDK L Q  +V  GT+++++TSEMFSK+    VEE   ++  V PP P+
Sbjct: 73  ALKEAPADRQCKDKLLFQCKVVEPGTMDKEVTSEMFSKEAGHRVEETIFKIIYVAPPQPQ 132

Query: 131 SP 132
           SP
Sbjct: 133 SP 134
[Close]
BLAST of Spo20149.1 vs. ExPASy Swiss-Prot
Match: VAP13_ARATH (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana GN=PVA13 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.900e-29
Identity = 66/128 (51.56%), Postives = 89/128 (69.53%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTD-QYVAFKVKTTSPKKYCVRPNVGVIQPKSIH 64
           + I P ELKF  EL KQS C+++L+NKT  Q VAFKVKTT+P+KYCVRPN GV+ P    
Sbjct: 7   VNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDSC 66

Query: 65  EFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNL 124
              V MQA K AP++  CKDKFLVQ+ +V +GT  +++ +EMF+K+  R +E+ K+RV  
Sbjct: 67  NVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVVY 126

Query: 125 VVPPPKSP 132
           +   P SP
Sbjct: 127 IPANPPSP 134
[Close]
BLAST of Spo20149.1 vs. TAIR (Arabidopsis)
Match: AT1G08820.1 (vamp/synaptobrevin-associated protein 27-2)

HSP 1 Score: 162.2 bits (409), Expect = 5.200e-40
Identity = 99/191 (51.83%), Postives = 124/191 (64.92%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           L+IQPR L+F V+L KQ+ C V+L+N T  YVAFKVKTTSPKKYCVRPNVGV+ PKS  E
Sbjct: 6   LDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAPKSTCE 65

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
           F VIMQA K  P +  CKDKFL+QST V   T +EDIT+ MFSK   +++EE K+RV L 
Sbjct: 66  FTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKLRVTL- 125

Query: 125 VPPPKSPELLPLNGTLKQADEHVESKTVNKEELKPVKDADVARPQDMNLEPIKQMKF--- 184
           VPP  SPEL P+N T KQ    V   ++ K+ L     ++   P     E +K+ +    
Sbjct: 126 VPPSDSPELSPIN-TPKQG--AVFEDSILKDRL--YSQSETLAPPQYEGEIVKEPRMVGH 185

Query: 185 DLSKAVDSAVE 193
           D  KA D+A E
Sbjct: 186 DELKAADNAKE 190
[Close]
BLAST of Spo20149.1 vs. TAIR (Arabidopsis)
Match: AT3G60600.1 (vesicle associated protein)

HSP 1 Score: 136.3 bits (342), Expect = 3.100e-32
Identity = 71/131 (54.20%), Postives = 89/131 (67.94%), Query Frame = 1 

		  

Query: 2   SSQLEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKS 61
           S  L ++P +L+F  EL KQ  C++ L+NKTD  VAFKVKTT+PKKYCVRPN GV+ P+S
Sbjct: 20  SELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRS 79

Query: 62  IHEFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRV 121
             E +V MQA K AP +  CKDKFL+Q  I   G   +++T EMFSK+    VEE K+RV
Sbjct: 80  TCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRV 139

Query: 122 NLVVPP-PKSP 132
             V PP P SP
Sbjct: 140 TYVAPPRPPSP 150
[Close]
BLAST of Spo20149.1 vs. TAIR (Arabidopsis)
Match: AT2G45140.1 (plant VAP homolog 12)

HSP 1 Score: 134.0 bits (336), Expect = 1.500e-31
Identity = 88/219 (40.18%), Postives = 122/219 (55.71%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHE 64
           L I P +L+F  EL KQ  C++ L NKTD YVAFKVKTT+PKKYCVRPN GV+ P+S  E
Sbjct: 6   LTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPRSSSE 65

Query: 65  FVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLV 124
            +V MQA K AP +  CKDKFL+Q  +   G   +D+T EMFSK+    VEE K+RV  V
Sbjct: 66  VLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKLRVVYV 125

Query: 125 VPP-PKSPELLPLNGTLKQADEHVESKTVNKEE--------LKPVKDADVARPQDMNLEP 184
            PP P SP        +++  E   S   +  +          P   AD    QD N   
Sbjct: 126 APPRPPSP--------VREGSEEGSSPRASVSDNGNASDFTAAPRFSADRVDAQD-NSSE 185

Query: 185 IKQMKFDLSKAVDSAVEKDVESELTKEIEPIIRNNEEPK 215
            + +   L++  +SAV+  + + L +E++ + R ++  K
Sbjct: 186 ARALVTKLTEEKNSAVQ--LNNRLQQELDQLRRESKRSK 213
[Close]
BLAST of Spo20149.1 vs. TAIR (Arabidopsis)
Match: AT1G51270.3 (structural molecules;transmembrane receptors;structural molecules)

HSP 1 Score: 133.3 bits (334), Expect = 2.600e-31
Identity = 64/122 (52.46%), Postives = 89/122 (72.95%), Query Frame = 1 

		  

Query: 11  ELKFVVELNKQSKCTVRLSNKTDQYVAFKVKTTSPKKYCVRPNVGVIQPKSIHEFVVIMQ 70
           +++F +ELNKQ  C++ L+NKTD YVAFK +TT PK YCV+P+VGV+ P+S  E +V+MQ
Sbjct: 13  DIRFPIELNKQGSCSLNLTNKTDNYVAFKAQTTKPKMYCVKPSVGVVLPRSSCEVLVVMQ 72

Query: 71  ALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNLVVPP-PK 130
           ALK AP +  CKDK L Q  +V  GT+++++TSEMFSK+    VEE   ++  V PP P+
Sbjct: 73  ALKEAPADRQCKDKLLFQCKVVEPGTMDKEVTSEMFSKEAGHRVEETIFKIIYVAPPQPQ 132

Query: 131 SP 132
           SP
Sbjct: 133 SP 134
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BLAST of Spo20149.1 vs. TAIR (Arabidopsis)
Match: AT4G00170.1 (Plant VAMP (vesicle-associated membrane protein) family protein)

HSP 1 Score: 130.2 bits (326), Expect = 2.200e-30
Identity = 66/128 (51.56%), Postives = 89/128 (69.53%), Query Frame = 1 

		  

Query: 5   LEIQPRELKFVVELNKQSKCTVRLSNKTD-QYVAFKVKTTSPKKYCVRPNVGVIQPKSIH 64
           + I P ELKF  EL KQS C+++L+NKT  Q VAFKVKTT+P+KYCVRPN GV+ P    
Sbjct: 7   VNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDSC 66

Query: 65  EFVVIMQALKTAPVETTCKDKFLVQSTIVPEGTVEEDITSEMFSKDGVRYVEEIKMRVNL 124
              V MQA K AP++  CKDKFLVQ+ +V +GT  +++ +EMF+K+  R +E+ K+RV  
Sbjct: 67  NVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVVY 126

Query: 125 VVPPPKSP 132
           +   P SP
Sbjct: 127 IPANPPSP 134
The following BLAST results are available for this feature:
BLAST of Spo20149.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902167614|gb|KNA07399.1|2.2e-15799.6hypothetical protein SOVF_1722... [more]
gi|731312073|ref|XP_010672484.1|2.0e-12681.1PREDICTED: vesicle-associated ... [more]
gi|661877819|emb|CDP18378.1|3.0e-6150.0unnamed protein product [Coffe... [more]
gi|1021052858|gb|KZN10636.1|4.3e-6044.0hypothetical protein DCAR_0032... [more]
gi|225432396|ref|XP_002277190.1|4.0e-5847.0PREDICTED: vesicle-associated ... [more]
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BLAST of Spo20149.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QJH0_SPIOL1.5e-15799.6Uncharacterized protein OS=Spi... [more]
A0A0J8D3D3_BETVU1.4e-12681.1Uncharacterized protein OS=Bet... [more]
A0A068VFA2_COFCA2.1e-6150.0Uncharacterized protein OS=Cof... [more]
A0A162ALM6_DAUCA3.0e-6044.0Uncharacterized protein OS=Dau... [more]
D7T6K0_VITVI2.8e-5847.0Putative uncharacterized prote... [more]
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BLAST of Spo20149.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
VAP22_ARATH9.3e-3951.8Vesicle-associated protein 2-2... [more]
VAP11_ARATH5.4e-3154.2Vesicle-associated protein 1-1... [more]
VAP12_ARATH2.7e-3040.1Vesicle-associated protein 1-2... [more]
VAP14_ARATH4.6e-3052.4Vesicle-associated protein 1-4... [more]
VAP13_ARATH3.9e-2951.5Vesicle-associated protein 1-3... [more]
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BLAST of Spo20149.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT1G08820.15.2e-4051.8vamp/synaptobrevin-associated ... [more]
AT3G60600.13.1e-3254.2vesicle associated protein[more]
AT2G45140.11.5e-3140.1plant VAP homolog 12[more]
AT1G51270.32.6e-3152.4structural molecules;transmemb... [more]
AT4G00170.12.2e-3051.5Plant VAMP (vesicle-associated... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 5..108
score: 7.4
IPR000535Major sperm protein (MSP) domainPROFILEPS50202MSPcoord: 4..124
score: 28
IPR008962PapD-likeGENE3D2.60.40.360coord: 3..122
score: 3.6
IPR008962PapD-likeunknownSSF49354PapD-likecoord: 2..131
score: 4.71
IPR016763Vesicle-associated membrane-protein-associated proteinPIRPIRSF019693VAMP_assoc_protcoord: 1..306
score: 2.1
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 211..306
score: 2.6E-104coord: 1..153
score: 2.6E
NoneNo IPR availableunknownCoilCoilcoord: 225..280
scor
NoneNo IPR availablePANTHERPTHR10809:SF45SUBFAMILY NOT NAMEDcoord: 211..306
score: 2.6E-104coord: 1..153
score: 2.6E

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032880 regulation of protein localization
biological_process GO:0006744 ubiquinone biosynthetic process
biological_process GO:0010304 PSII associated light-harvesting complex II catabolic process
biological_process GO:0006508 proteolysis
biological_process GO:0051301 cell division
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006657 CDP-choline pathway
biological_process GO:0010286 heat acclimation
biological_process GO:0009987 cellular process
biological_process GO:0009395 phospholipid catabolic process
biological_process GO:0006655 phosphatidylglycerol biosynthetic process
biological_process GO:0006281 DNA repair
biological_process GO:0007064 mitotic sister chromatid cohesion
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0042908 xenobiotic transport
biological_process GO:0016573 histone acetylation
biological_process GO:0007165 signal transduction
biological_process GO:0010260 animal organ senescence
biological_process GO:0006468 protein phosphorylation
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006886 intracellular protein transport
biological_process GO:0006855 drug transmembrane transport
biological_process GO:0019761 glucosinolate biosynthetic process
biological_process GO:0000023 maltose metabolic process
biological_process GO:0019252 starch biosynthetic process
biological_process GO:0009408 response to heat
cellular_component GO:0008278 cohesin complex
cellular_component GO:0005886 plasma membrane
cellular_component GO:0000785 chromatin
cellular_component GO:0005634 nucleus
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005739 mitochondrion
cellular_component GO:0009507 chloroplast
cellular_component GO:0005622 intracellular
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008375 acetylglucosaminyltransferase activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0042802 identical protein binding
molecular_function GO:0003682 chromatin binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0008559 xenobiotic transmembrane transporting ATPase activity
molecular_function GO:0050200 plasmalogen synthase activity
molecular_function GO:0004105 choline-phosphate cytidylyltransferase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008568 microtubule-severing ATPase activity
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0005488 binding
RNA-Seq Expression