Spo20143 (gene) | SpinachBase

Overview


Name	Spo20143
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	(Blue copper protein, putative) (Plastocyanin-like domain-containing protein)
Location	chr3 : 108646670 .. 108646854 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAATTGAAGAGAATGTCGATGATGGTGGCGTTGGTTTTTGTGGTTTTGATTACTGAACAAGTTTGTGCAACACAACATATTGTGGGTGGAAGTGGTGGTTGGGATGAGTCTACAGATTATGATTCATGGGCTAAAGGACAGACTTTTAAAGTTGGTGATACACTTGGTAAGTTCGATAA

mRNA sequence

ATGGGAGAATTGAAGAGAATGTCGATGATGGTGGCGTTGGTTTTTGTGGTTTTGATTACTGAACAAGTTTGTGCAACACAACATATTGTGGGTGGAAGTGGTGGTTGGGATGAGTCTACAGATTATGATTCATGGGCTAAAGGACAGACTTTTAAAGTTGGTGATACACTTGTTCGATAA

Coding sequence (CDS)

ATGGGAGAATTGAAGAGAATGTCGATGATGGTGGCGTTGGTTTTTGTGGTTTTGATTACTGAACAAGTTTGTGCAACACAACATATTGTGGGTGGAAGTGGTGGTTGGGATGAGTCTACAGATTATGATTCATGGGCTAAAGGACAGACTTTTAAAGTTGGTGATACACTTGTTCGATAA

Protein sequence

MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLVR

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo20143.1	Spo20143.1	mRNA

Homology

[Close]

BLAST of Spo20143.1 vs. NCBI nr
Match: gi|902219839|gb|KNA18428.1| (hypothetical protein SOVF_070830 [Spinacia oleracea])

HSP 1 Score: 119.4 bits (298), Expect = 2.100e-24
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 1


		  
Query: 1  MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV
Sbjct: 1  MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 58

[Close]

BLAST of Spo20143.1 vs. NCBI nr
Match: gi|731319714|ref|XP_010670421.1| (PREDICTED: basic blue protein-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 82.0 bits (201), Expect = 3.800e-13
Identity = 42/58 (72.41%), Postives = 47/58 (81.03%), Query Frame = 1


		  
Query: 1  MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          M  LKR   ++ALV +V IT Q  ATQH+VGGS GWDESTDY+SWAKGQTFKVGDTLV
Sbjct: 1  MEGLKRR--LLALVLMVCITTQAYATQHVVGGSDGWDESTDYNSWAKGQTFKVGDTLV 56

[Close]

BLAST of Spo20143.1 vs. NCBI nr
Match: gi|970006553|ref|XP_015064132.1| (PREDICTED: mavicyanin-like [Solanum pennellii])

HSP 1 Score: 73.2 bits (178), Expect = 1.700e-10
Identity = 32/55 (58.18%), Postives = 43/55 (78.18%), Query Frame = 1


		  
Query: 4  LKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          L R S++++++ +V+I +   A QH+VGGS GWDES D+ SWA GQTFKVGDTLV
Sbjct: 3  LLRKSLLISVIVMVMIQKNAMAAQHVVGGSQGWDESADFKSWASGQTFKVGDTLV 57

[Close]

BLAST of Spo20143.1 vs. NCBI nr
Match: gi|460371930|ref|XP_004231787.1| (PREDICTED: mavicyanin [Solanum lycopersicum])

HSP 1 Score: 73.2 bits (178), Expect = 1.700e-10
Identity = 32/55 (58.18%), Postives = 43/55 (78.18%), Query Frame = 1


		  
Query: 4  LKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          L R S++++++ +V+I +   A QH+VGGS GWDES D+ SWA GQTFKVGDTLV
Sbjct: 3  LVRKSLLISVIVMVMIQKNAMAAQHVVGGSQGWDESADFKSWASGQTFKVGDTLV 57

[Close]

BLAST of Spo20143.1 vs. NCBI nr
Match: gi|697121793|ref|XP_009614877.1| (PREDICTED: basic blue protein-like [Nicotiana tomentosiformis])

HSP 1 Score: 72.4 bits (176), Expect = 3.000e-10
Identity = 31/56 (55.36%), Postives = 42/56 (75.00%), Query Frame = 1


		  
Query: 3  ELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          E  R S+++  +  V+I  +  A QH+VGGS GWDESTD++SWA G+TFK+GDTLV
Sbjct: 2  EALRKSLLIFAIVTVIIQNKAMAAQHVVGGSQGWDESTDFNSWASGETFKIGDTLV 57

[Close]

BLAST of Spo20143.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RG09_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_070830 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.500e-24
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 1


		  
Query: 1  MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV
Sbjct: 1  MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 58

[Close]

BLAST of Spo20143.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CTJ2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g041160 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.600e-13
Identity = 42/58 (72.41%), Postives = 47/58 (81.03%), Query Frame = 1


		  
Query: 1  MGELKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          M  LKR   ++ALV +V IT Q  ATQH+VGGS GWDESTDY+SWAKGQTFKVGDTLV
Sbjct: 1  MEGLKRR--LLALVLMVCITTQAYATQHVVGGSDGWDESTDYNSWAKGQTFKVGDTLV 56

[Close]

BLAST of Spo20143.1 vs. UniProtKB/TrEMBL
Match: K4BDK1_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.200e-10
Identity = 32/55 (58.18%), Postives = 43/55 (78.18%), Query Frame = 1


		  
Query: 4  LKRMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          L R S++++++ +V+I +   A QH+VGGS GWDES D+ SWA GQTFKVGDTLV
Sbjct: 3  LVRKSLLISVIVMVMIQKNAMAAQHVVGGSQGWDESADFKSWASGQTFKVGDTLV 57

[Close]

BLAST of Spo20143.1 vs. UniProtKB/TrEMBL
Match: A0A151REF0_CAJCA (Blue copper protein OS=Cajanus cajan GN=KK1_037617 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.100e-10
Identity = 33/53 (62.26%), Postives = 40/53 (75.47%), Query Frame = 1


		  
Query: 6  RMSMMVALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          RM+ +   +FV LIT+   A QH+VGGS GWDESTD++SW  GQTFKVGD LV
Sbjct: 3  RMNTIFLALFVALITKDAMAEQHVVGGSQGWDESTDFNSWVSGQTFKVGDQLV 55

[Close]

BLAST of Spo20143.1 vs. UniProtKB/TrEMBL
Match: B9GJS1_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s33960g PE=4 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 3.500e-10
Identity = 32/48 (66.67%), Postives = 38/48 (79.17%), Query Frame = 1


		  
Query: 11 VALVFVVLITEQVCATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          + LVFV LIT++  A QH+VGGS GW+ESTD+ SWA GQ FKVGD LV
Sbjct: 7  LVLVFVALITKEAMAAQHVVGGSQGWEESTDFSSWASGQKFKVGDQLV 54

[Close]

BLAST of Spo20143.1 vs. TAIR (Arabidopsis)
Match: AT3G27200.1 (Cupredoxin superfamily protein)

HSP 1 Score: 57.0 bits (136), Expect = 4.600e-9
Identity = 25/56 (44.64%), Postives = 41/56 (73.21%), Query Frame = 1


		  
Query: 4  LKRMSMMVALVFVVLITEQVC-ATQHIVGGSGGWDESTDYDSWAKGQTFKVGDTLV 59
          +K  +++V LVF  L++ +   A +H++GGS GW++S D+DSW+  Q+FKVGD +V
Sbjct: 1  MKMQAVLVILVFSGLLSVKTALAARHVIGGSQGWEQSVDFDSWSSDQSFKVGDQIV 56

The following BLAST results are available for this feature:

BLAST of Spo20143.1 vs. NCBI nr
BLAST of Spo20143.1 vs. UniProtKB/TrEMBL
BLAST of Spo20143.1 vs. ExPASy Swiss-Prot
BLAST of Spo20143.1 vs. TAIR (Arabidopsis)

BLAST of Spo20143.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902219839\|gb\|KNA18428.1\|	2.1e-24	100.	hypothetical protein SOVF_0708...	[more]
gi\|731319714\|ref\|XP_010670421.1\|	3.8e-13	72.4	PREDICTED: basic blue protein-...	[more]
gi\|970006553\|ref\|XP_015064132.1\|	1.7e-10	58.1	PREDICTED: mavicyanin-like [So...	[more]
gi\|460371930\|ref\|XP_004231787.1\|	1.7e-10	58.1	PREDICTED: mavicyanin [Solanum...	[more]
gi\|697121793\|ref\|XP_009614877.1\|	3.0e-10	55.3	PREDICTED: basic blue protein-...	[more]

BLAST of Spo20143.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RG09_SPIOL	1.5e-24	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CTJ2_BETVU	2.6e-13	72.4	Uncharacterized protein OS=Bet...	[more]
K4BDK1_SOLLC	1.2e-10	58.1	Uncharacterized protein OS=Sol...	[more]
A0A151REF0_CAJCA	2.1e-10	62.2	Blue copper protein OS=Cajanus...	[more]
B9GJS1_POPTR	3.5e-10	66.6	Uncharacterized protein OS=Pop...	[more]

BLAST of Spo20143.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo20143.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G27200.1	4.6e-9	44.6	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 37..58 score: 1.
IPR003245	Phytocyanin domain	PROFILE	PS51485	PHYTOCYANIN	coord: 26..59 score: 16
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 25..58 score: 1.
IPR008972	Cupredoxin	unknown	SSF49503	Cupredoxins	coord: 25..58 score: 2.0
None	No IPR available	PANTHER	PTHR33021	FAMILY NOT NAMED	coord: 9..58 score: 6.0
None	No IPR available	PANTHER	PTHR33021:SF50	SUBFAMILY NOT NAMED	coord: 9..58 score: 6.0

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010413	glucuronoxylan metabolic process
biological_process	GO:0045492	xylan biosynthetic process
biological_process	GO:0045491	xylan metabolic process
cellular_component	GO:0031225	anchored component of membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0009055	electron transfer activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo06255	0.79	Barchart \| Table
Spo24856	0.69	Barchart \| Table