Spo19253 (gene) | SpinachBase

Overview


Name	Spo19253
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	50S ribosomal protein L7/L12, putative
Location	chr5 : 6733599 .. 6734162 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATTTCATTAAGCATTTAAGAAATATACATATAAAGTCCATATTCCAAGAAAAACATGTCCTGTTTCAAGCCCGTTGTCTGCAAACTGACGTATCCAGCAAGCCTAAGAGGTACAAGTACCCCACGGCCTACGACCCATATGGGCCAAGGCCACCACCCTCAGAAAAAGTCACTCAACTAGCTGAACAAATCATAACCCTAACTTCTGAAGAACGAGCTCAAATCGGTCCAGCACTCAGAGAACTACTCAAACATCCCAAGTTGGAAAAGATTTCAACAGAAGGCTTAGACTTGGGTGGTGCAGCTGGTGGAGCCACCACCGCGGCAGAGGAGAAGGTGGCAGAAAAAACCGCTTTCGATGTGAAGTTGGAGAAGTTTGATGCTACTTCGAAGCTCAAGGTGATCAAGGAAGTAAGAGCTTTGACCAGTCTAGGACTGAAGGAAGCCAAAGAGCTAGTTGAAAAAGCACCAGTTGTACTTAAACAGGGACTCACAAAAGAAGAGGCAGAAGGTATGATTGAGAAGATTAAGGCTGCTGGTGGATCTGCAGTAATGGAGTGA

mRNA sequence

ATGCATTTCATTAAGCATTTAAGAAATATACATATAAAGTCCATATTCCAAGAAAAACATGTCCTGTTTCAAGCCCGTTGTCTGCAAACTGACGTATCCAGCAAGCCTAAGAGGTACAAGTACCCCACGGCCTACGACCCATATGGGCCAAGGCCACCACCCTCAGAAAAAGTCACTCAACTAGCTGAACAAATCATAACCCTAACTTCTGAAGAACGAGCTCAAATCGGTCCAGCACTCAGAGAACTACTCAAACATCCCAAGTTGGAAAAGATTTCAACAGAAGGCTTAGACTTGGGTGGTGCAGCTGGTGGAGCCACCACCGCGGCAGAGGAGAAGGTGGCAGAAAAAACCGCTTTCGATGTGAAGTTGGAGAAGTTTGATGCTACTTCGAAGCTCAAGGTGATCAAGGAAGTAAGAGCTTTGACCAGTCTAGGACTGAAGGAAGCCAAAGAGCTAGTTGAAAAAGCACCAGTTGTACTTAAACAGGGACTCACAAAAGAAGAGGCAGAAGGTATGATTGAGAAGATTAAGGCTGCTGGTGGATCTGCAGTAATGGAGTGA

Coding sequence (CDS)

ATGCATTTCATTAAGCATTTAAGAAATATACATATAAAGTCCATATTCCAAGAAAAACATGTCCTGTTTCAAGCCCGTTGTCTGCAAACTGACGTATCCAGCAAGCCTAAGAGGTACAAGTACCCCACGGCCTACGACCCATATGGGCCAAGGCCACCACCCTCAGAAAAAGTCACTCAACTAGCTGAACAAATCATAACCCTAACTTCTGAAGAACGAGCTCAAATCGGTCCAGCACTCAGAGAACTACTCAAACATCCCAAGTTGGAAAAGATTTCAACAGAAGGCTTAGACTTGGGTGGTGCAGCTGGTGGAGCCACCACCGCGGCAGAGGAGAAGGTGGCAGAAAAAACCGCTTTCGATGTGAAGTTGGAGAAGTTTGATGCTACTTCGAAGCTCAAGGTGATCAAGGAAGTAAGAGCTTTGACCAGTCTAGGACTGAAGGAAGCCAAAGAGCTAGTTGAAAAAGCACCAGTTGTACTTAAACAGGGACTCACAAAAGAAGAGGCAGAAGGTATGATTGAGAAGATTAAGGCTGCTGGTGGATCTGCAGTAATGGAGTGA

Protein sequence

MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAAGGSAVME

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo19253.1	Spo19253.1	mRNA

Homology

[Close]

BLAST of Spo19253.1 vs. NCBI nr
Match: gi|902239814|gb|KNA25741.1| (hypothetical protein SOVF_004040 [Spinacia oleracea])

HSP 1 Score: 351.7 bits (901), Expect = 8.100e-94
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQ 60
           MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQ
Sbjct: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQ 60

Query: 61  LAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKTAF 120
           LAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKTAF
Sbjct: 61  LAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKTAF 120

Query: 121 DVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAA 180
           DVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAA
Sbjct: 121 DVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAA 180

Query: 181 GGSAVME 188
           GGSAVME
Sbjct: 181 GGSAVME 187

[Close]

BLAST of Spo19253.1 vs. NCBI nr
Match: gi|731335536|ref|XP_010678774.1| (PREDICTED: 50S ribosomal protein L12, cyanelle [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 284.3 bits (726), Expect = 1.600e-73
Identity = 147/189 (77.78%), Postives = 170/189 (89.95%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVS--SKPKRYKYPTAYDPYGPRPPPSEKV 60
           MH I+HLRNIH+K IFQEK+VL Q RCLQTD++  +KPK+YKYP+ Y+PYG RPPPSEKV
Sbjct: 1   MHLIRHLRNIHVKRIFQEKYVLSQVRCLQTDIAQKTKPKKYKYPSVYEPYGTRPPPSEKV 60

Query: 61  TQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKT 120
           + LAEQI+TLT+EERAQIGPALRE+LKHP+LEKISTEGLDLG  AGGA   AEEK  E+T
Sbjct: 61  SLLAEQIVTLTAEERAQIGPALREILKHPELEKISTEGLDLGAPAGGAAAQAEEKKEERT 120

Query: 121 AFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIK 180
           AFDVKLEKFD  SK+KVIKEVRALT+LGLKEAK+LVEKAPV+LKQG+TKEEA G+IEKIK
Sbjct: 121 AFDVKLEKFDTASKIKVIKEVRALTNLGLKEAKDLVEKAPVILKQGVTKEEAAGIIEKIK 180

Query: 181 AAGGSAVME 188
           AAGG+AV+E
Sbjct: 181 AAGGAAVIE 189

[Close]

BLAST of Spo19253.1 vs. NCBI nr
Match: gi|702325563|ref|XP_010053490.1| (PREDICTED: 50S ribosomal protein L12, chloroplastic [Eucalyptus grandis])

HSP 1 Score: 226.9 bits (577), Expect = 3.000e-56
Identity = 120/167 (71.86%), Postives = 140/167 (83.83%), Query Frame = 1


		  
Query: 23  FQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQLAEQIITLTSEERAQIGPALRE 82
           FQ   +  D  +KPK+YKYP  YDPYGPRPPPS+K+ QLAE+I  L +EERAQIGPAL+E
Sbjct: 33  FQHDFVPRDPKAKPKKYKYPAFYDPYGPRPPPSDKIIQLAERIAGLPAEERAQIGPALQE 92

Query: 83  LLKHPKLEKISTEGLDLG--GAAGGATTAAEEKVAEKTAFDVKLEKFDATSKLKVIKEVR 142
            LKHPK++ ISTEG+DLG  G AG  +  AEEK AEKTAFDVKLEKFDA +K+KVIKEVR
Sbjct: 93  RLKHPKMQTISTEGMDLGPQGGAGAGSAKAEEKKAEKTAFDVKLEKFDAAAKIKVIKEVR 152

Query: 143 ALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAAGGSAVME 188
           A T+LGLKEAK+LVEKAP++LKQG+TKEEA G+IEKIKAAGG AVME
Sbjct: 153 AFTNLGLKEAKDLVEKAPILLKQGVTKEEANGIIEKIKAAGGVAVME 199

[Close]

BLAST of Spo19253.1 vs. NCBI nr
Match: gi|697182448|ref|XP_009600228.1| (PREDICTED: 50S ribosomal protein L12, chloroplastic {ECO:0000255|HAMAP-Rule:MF_00368}-like [Nicotiana tomentosiformis])

HSP 1 Score: 221.5 bits (563), Expect = 1.300e-54
Identity = 124/194 (63.92%), Postives = 145/194 (74.74%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDV-----SSKPKRYKYPTAYDPYGPRPPPS 60
           M  I  LR IH  +  +E     Q+R  Q D      ++KPKRYKYP +YDPYGPR PPS
Sbjct: 1   MKLIARLRRIHACTSLREIIGPLQSRSFQPDFVPRDPNAKPKRYKYPASYDPYGPRAPPS 60

Query: 61  EKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLG--GAAGGATTAAEEK 120
           +K+ QLAE+I  L  EER QIGP LRE+L+HP L++IS EG+DLG  G AGG    AEEK
Sbjct: 61  DKIIQLAERIAALAPEERRQIGPTLREILRHPNLKQISVEGMDLGAMGGAGGGPAKAEEK 120

Query: 121 VAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGM 180
            AEKTAFDVKLEKFDA +K+KVIKEVR+ TSLGLKEAK+LVEK P +LKQG+TKEEA  +
Sbjct: 121 KAEKTAFDVKLEKFDAAAKIKVIKEVRSFTSLGLKEAKDLVEKVPAILKQGVTKEEANEI 180

Query: 181 IEKIKAAGGSAVME 188
           IEKIKAAGG AVME
Sbjct: 181 IEKIKAAGGVAVME 194

[Close]

BLAST of Spo19253.1 vs. NCBI nr
Match: gi|565348179|ref|XP_006341095.1| (PREDICTED: 50S ribosomal protein L7/L12 [Solanum tuberosum])

HSP 1 Score: 219.9 bits (559), Expect = 3.700e-54
Identity = 125/194 (64.43%), Postives = 143/194 (73.71%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDV-----SSKPKRYKYPTAYDPYGPRPPPS 60
           M  I  LR I   +  +E     Q+R  Q D      ++KP RYKYP AYDPYGPRPPPS
Sbjct: 1   MKLIARLRPIQTCTALREIIGPLQSRSFQPDFVPRDPNAKPIRYKYPAAYDPYGPRPPPS 60

Query: 61  EKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLG--GAAGGATTAAEEK 120
           +K+ QLAE+I  L  EER QIGP LRE+L+HP L++IS EG+DLG  G AGG    AEEK
Sbjct: 61  DKIVQLAERIAALAPEERRQIGPLLREILRHPNLKQISVEGMDLGAMGGAGGGPAKAEEK 120

Query: 121 VAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGM 180
             EKTAFDVKLEKFDA SK+KVIKEVR+ TSLGLKEAKELVEKAP +LKQG+TKEEA  +
Sbjct: 121 KVEKTAFDVKLEKFDAASKIKVIKEVRSFTSLGLKEAKELVEKAPAILKQGVTKEEANEI 180

Query: 181 IEKIKAAGGSAVME 188
           IEKIKA GG AVME
Sbjct: 181 IEKIKAVGGVAVME 194

[Close]

BLAST of Spo19253.1 vs. UniProtKB/TrEMBL
Match: A0A0K9S1W9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_004040 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 5.600e-94
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQ 60
           MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQ
Sbjct: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQ 60

Query: 61  LAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKTAF 120
           LAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKTAF
Sbjct: 61  LAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKTAF 120

Query: 121 DVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAA 180
           DVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAA
Sbjct: 121 DVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAA 180

Query: 181 GGSAVME 188
           GGSAVME
Sbjct: 181 GGSAVME 187

[Close]

BLAST of Spo19253.1 vs. UniProtKB/TrEMBL
Match: A0A0J8F4B8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g117170 PE=4 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.100e-73
Identity = 147/189 (77.78%), Postives = 170/189 (89.95%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVS--SKPKRYKYPTAYDPYGPRPPPSEKV 60
           MH I+HLRNIH+K IFQEK+VL Q RCLQTD++  +KPK+YKYP+ Y+PYG RPPPSEKV
Sbjct: 1   MHLIRHLRNIHVKRIFQEKYVLSQVRCLQTDIAQKTKPKKYKYPSVYEPYGTRPPPSEKV 60

Query: 61  TQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKT 120
           + LAEQI+TLT+EERAQIGPALRE+LKHP+LEKISTEGLDLG  AGGA   AEEK  E+T
Sbjct: 61  SLLAEQIVTLTAEERAQIGPALREILKHPELEKISTEGLDLGAPAGGAAAQAEEKKEERT 120

Query: 121 AFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIK 180
           AFDVKLEKFD  SK+KVIKEVRALT+LGLKEAK+LVEKAPV+LKQG+TKEEA G+IEKIK
Sbjct: 121 AFDVKLEKFDTASKIKVIKEVRALTNLGLKEAKDLVEKAPVILKQGVTKEEAAGIIEKIK 180

Query: 181 AAGGSAVME 188
           AAGG+AV+E
Sbjct: 181 AAGGAAVIE 189

[Close]

BLAST of Spo19253.1 vs. UniProtKB/TrEMBL
Match: A0A059CHD8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02092 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.100e-56
Identity = 120/167 (71.86%), Postives = 140/167 (83.83%), Query Frame = 1


		  
Query: 23  FQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQLAEQIITLTSEERAQIGPALRE 82
           FQ   +  D  +KPK+YKYP  YDPYGPRPPPS+K+ QLAE+I  L +EERAQIGPAL+E
Sbjct: 33  FQHDFVPRDPKAKPKKYKYPAFYDPYGPRPPPSDKIIQLAERIAGLPAEERAQIGPALQE 92

Query: 83  LLKHPKLEKISTEGLDLG--GAAGGATTAAEEKVAEKTAFDVKLEKFDATSKLKVIKEVR 142
            LKHPK++ ISTEG+DLG  G AG  +  AEEK AEKTAFDVKLEKFDA +K+KVIKEVR
Sbjct: 93  RLKHPKMQTISTEGMDLGPQGGAGAGSAKAEEKKAEKTAFDVKLEKFDAAAKIKVIKEVR 152

Query: 143 ALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAAGGSAVME 188
           A T+LGLKEAK+LVEKAP++LKQG+TKEEA G+IEKIKAAGG AVME
Sbjct: 153 AFTNLGLKEAKDLVEKAPILLKQGVTKEEANGIIEKIKAAGGVAVME 199

[Close]

BLAST of Spo19253.1 vs. UniProtKB/TrEMBL
Match: A0A0V0HUT6_SOLCH (Putative 54S ribosomal protein L12, mitochondrial-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 3.000e-55
Identity = 126/194 (64.95%), Postives = 145/194 (74.74%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDV-----SSKPKRYKYPTAYDPYGPRPPPS 60
           M  I  LR I   +  +E     Q+R  Q D      ++KP RYKYP AYDPYGPRPPPS
Sbjct: 1   MKLIARLRPIQTCTALREIIGPLQSRSFQPDFVPRDPNAKPIRYKYPAAYDPYGPRPPPS 60

Query: 61  EKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLG--GAAGGATTAAEEK 120
           +K+ QLAE+I  L  EER QIGP LRE+L+HP L++IS EG+DLG  G AGG TT AEEK
Sbjct: 61  DKIVQLAERIAALAPEERRQIGPLLREILRHPNLKQISVEGMDLGAMGGAGGGTTKAEEK 120

Query: 121 VAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGM 180
             EKTAFDVKLEKFDA +K+KVIKEVR+ TSLGLKEAKELVEKAP +LKQG+TKEEA  +
Sbjct: 121 KVEKTAFDVKLEKFDAAAKIKVIKEVRSFTSLGLKEAKELVEKAPAILKQGVTKEEANEI 180

Query: 181 IEKIKAAGGSAVME 188
           IEKIKA GG AVME
Sbjct: 181 IEKIKAVGGVAVME 194

[Close]

BLAST of Spo19253.1 vs. UniProtKB/TrEMBL
Match: M1AHC8_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400008867 PE=4 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.500e-54
Identity = 125/194 (64.43%), Postives = 143/194 (73.71%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDV-----SSKPKRYKYPTAYDPYGPRPPPS 60
           M  I  LR I   +  +E     Q+R  Q D      ++KP RYKYP AYDPYGPRPPPS
Sbjct: 1   MKLIARLRPIQTCTALREIIGPLQSRSFQPDFVPRDPNAKPIRYKYPAAYDPYGPRPPPS 60

Query: 61  EKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLG--GAAGGATTAAEEK 120
           +K+ QLAE+I  L  EER QIGP LRE+L+HP L++IS EG+DLG  G AGG    AEEK
Sbjct: 61  DKIVQLAERIAALAPEERRQIGPLLREILRHPNLKQISVEGMDLGAMGGAGGGPAKAEEK 120

Query: 121 VAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGM 180
             EKTAFDVKLEKFDA SK+KVIKEVR+ TSLGLKEAKELVEKAP +LKQG+TKEEA  +
Sbjct: 121 KVEKTAFDVKLEKFDAASKIKVIKEVRSFTSLGLKEAKELVEKAPAILKQGVTKEEANEI 180

Query: 181 IEKIKAAGGSAVME 188
           IEKIKA GG AVME
Sbjct: 181 IEKIKAVGGVAVME 194

[Close]

BLAST of Spo19253.1 vs. ExPASy Swiss-Prot
Match: RK12_PHATC (50S ribosomal protein L12, chloroplastic OS=Phaeodactylum tricornutum (strain CCAP 1055/1) GN=rpl12 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.700e-15
Identity = 57/135 (42.22%), Postives = 80/135 (59.26%), Query Frame = 1


		  
Query: 55  SEKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGG-----ATTA 114
           SEK+ Q+ E + TLT  E +++   + E           T G+D   AA G     A  A
Sbjct: 2   SEKIDQIVEDLKTLTLLEASELVTKIEE-----------TFGVDASAAASGGVVMAAAPA 61

Query: 115 AEEKVAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEE 174
             E+V EKT F++ L++  A  K+ V+K VR+LT LGLKEAKELVE AP  +++GL K+ 
Sbjct: 62  VVEEVEEKTEFNLMLDEVPADKKIAVLKVVRSLTGLGLKEAKELVESAPKQIQEGLGKDA 121

Query: 175 AEGMIEKIKAAGGSA 185
           AE   ++I+AAGG A
Sbjct: 122 AEEAKKQIEAAGGKA 125

[Close]

BLAST of Spo19253.1 vs. ExPASy Swiss-Prot
Match: RK12_THAPS (50S ribosomal protein L12, chloroplastic OS=Thalassiosira pseudonana GN=rpl12 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.400e-15
Identity = 55/133 (41.35%), Postives = 78/133 (58.65%), Query Frame = 1


		  
Query: 55  SEKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATT-----A 114
           SEK+ Q+ E++ TLT  E +++  A+ E           T G+D   + GG        A
Sbjct: 2   SEKINQIVEELKTLTLLEASELVAAIEE-----------TFGVDASASTGGGVVMAVAPA 61

Query: 115 AEEKVAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEE 174
           A E+V EKT F V L++  A  K+ V+K VR LT LGLKEAKELVE  P ++++GL K+ 
Sbjct: 62  AAEEVEEKTEFTVMLDEVPADKKIAVLKVVRTLTGLGLKEAKELVESTPKMVQEGLGKDA 121

Query: 175 AEGMIEKIKAAGG 183
           AE   ++I+ AGG
Sbjct: 122 AEDAKKQIEDAGG 123

[Close]

BLAST of Spo19253.1 vs. ExPASy Swiss-Prot
Match: RL7_PROM4 (50S ribosomal protein L7/L12 OS=Prochlorococcus marinus (strain MIT 9211) GN=rplL PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 9.700e-15
Identity = 51/134 (38.06%), Postives = 81/134 (60.45%), Query Frame = 1


		  
Query: 55  SEKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGG-AAGGATTAAEEK 114
           S+K  ++ + + +L+  E +++   + E      +   ++ G+ +    AGG  +AA E 
Sbjct: 2   SKKTDEILDSLKSLSLLEASELVKQIEEAFG---VSAAASAGVVMAAPGAGGGASAAGEA 61

Query: 115 VAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGM 174
             EKT FDV LE FDA +K+KV+KEVR  T LGL EAK +VE AP  +K+G +KE+AE +
Sbjct: 62  AEEKTEFDVILESFDAAAKIKVLKEVRNATGLGLGEAKAMVEAAPKTIKEGASKEDAEAL 121

Query: 175 IEKIKAAGGSAVME 188
            + I+A GG   ++
Sbjct: 122 KKAIEAVGGKVTLK 132

[Close]

BLAST of Spo19253.1 vs. ExPASy Swiss-Prot
Match: RL7_LIBAF (50S ribosomal protein L7/L12 OS=Liberibacter africanus GN=rplL PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.300e-14
Identity = 53/129 (41.09%), Postives = 79/129 (61.24%), Query Frame = 1


		  
Query: 58  VTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEK 117
           +  + E++ +LT  + A++   L E         ++     +  AAG +  A    VAEK
Sbjct: 4   IESIVEKLSSLTLLQAAELSKRLEEEWGVSAAAPVAV----VASAAGESAAA----VAEK 63

Query: 118 TAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKI 177
           T F+V LE FDA  K+ VIKEVRA+T LGLKEAK+ VE AP  LK G++K+EAE + +K+
Sbjct: 64  TEFEVFLEGFDAKKKISVIKEVRAITELGLKEAKDFVESAPKSLKTGVSKDEAEELKKKL 123

Query: 178 KAAGGSAVM 187
           +AAG + ++
Sbjct: 124 EAAGATIIL 124

[Close]

BLAST of Spo19253.1 vs. ExPASy Swiss-Prot
Match: RK12_ODOSI (50S ribosomal protein L12, chloroplastic OS=Odontella sinensis GN=rpl12 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.200e-14
Identity = 55/137 (40.15%), Postives = 81/137 (59.12%), Query Frame = 1


		  
Query: 55  SEKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGG----ATTAA 114
           SEK+ Q+ E++ TLT  E +++   + E           T  +D   + GG    A  A 
Sbjct: 2   SEKIDQIVEELKTLTLLEASELVSKIEE-----------TFDVDASASVGGGMMMAGPAV 61

Query: 115 EEKVAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEA 174
            E+V EKT FDV L++  A  K+ V+K VR+LT LGLKEAKELVE AP  +++G+ K+ A
Sbjct: 62  VEEVEEKTEFDVMLDEVPADKKIAVLKVVRSLTGLGLKEAKELVESAPKQIQEGVAKDAA 121

Query: 175 EGMIEKIKAAGGSAVME 188
           E   ++I+ AGG A ++
Sbjct: 122 EEAKKQIEDAGGKASLK 127

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BLAST of Spo19253.1 vs. TAIR (Arabidopsis)
Match: AT3G06040.1 (Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family protein)

HSP 1 Score: 183.0 bits (463), Expect = 1.700e-46
Identity = 109/189 (57.67%), Postives = 130/189 (68.78%), Query Frame = 1


		  
Query: 1   MHFIKHLRNIHIKSIFQEKHVLFQARCLQTDVSSK--PKRYKYPTAYDPYGPRPPPSEKV 60
           M  I  +RN+  +    E     Q R LQ D  SK  PK+YKYP+ YDPYGPRP PS K+
Sbjct: 1   MKLISLVRNVRSRQCQPEVIWSVQVRFLQQDSVSKAKPKKYKYPSVYDPYGPRPQPSSKI 60

Query: 61  TQLAEQIITLTSEERAQIGPALRELLKHPKLEKISTEGLDLGGAAGGATTAAEEKVAEKT 120
            +LAE+I  L+ EER QIGPAL E L+ PK + IS++G+   GA         E+  EKT
Sbjct: 61  MELAERIAALSPEERKQIGPALNEHLRLPKQQMISSDGI---GAKQDTGAGNVEEKKEKT 120

Query: 121 AFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIK 180
           AFDVKLEKF+A+ K+KVIKEVR  TSLGLKEAKELVEK P +LKQG+TKEEA  +I KIK
Sbjct: 121 AFDVKLEKFNASDKIKVIKEVRTFTSLGLKEAKELVEKVPAILKQGVTKEEANEIIAKIK 180

Query: 181 AAGGSAVME 188
           A GG AVME
Sbjct: 181 AVGGIAVME 186

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BLAST of Spo19253.1 vs. TAIR (Arabidopsis)
Match: AT1G70190.1 (Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like)

HSP 1 Score: 110.9 bits (276), Expect = 8.400e-25
Identity = 70/181 (38.67%), Postives = 107/181 (59.12%), Query Frame = 1


		  
Query: 15  IFQEKHVLFQARCLQTDVSSKPKRYKYPTAYDPYGPRPPPSEKVTQLAEQIITLTSEERA 74
           I  E+   F  R L TD          P  +DP   R PP+++V +L ++I +LT  E +
Sbjct: 36  IEDEEEEEFDQRKLPTDYD--------PATFDPTEHRSPPTDRVFRLVDEISSLTLLEIS 95

Query: 75  QIGPALRELLKHPKLEKISTE------GLDLGGAAGGATTA--AEEKVAEKTAFDVKLEK 134
           ++G  + +     +L  ++        G+ +GG  G  + +  +EE   EKT F++KLE 
Sbjct: 96  ELGSIIMKKKGMTELPTVAVMKPGAGGGVGVGGGGGMVSQSGVSEEAKVEKTVFEIKLES 155

Query: 135 FDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLKQGLTKEEAEGMIEKIKAAGGSAVM 188
           F+A++K+K+IKE+R+ T LGLKEAK LVEK P +LK GL+KEE E ++EK+KA G   V+
Sbjct: 156 FEASAKIKIIKELRSFTDLGLKEAKALVEKTPAILKAGLSKEEGEKILEKLKALGAKVVL 208

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BLAST of Spo19253.1 vs. TAIR (Arabidopsis)
Match: AT4G37660.1 (Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family protein)

HSP 1 Score: 107.1 bits (266), Expect = 1.200e-23
Identity = 57/87 (65.52%), Postives = 69/87 (79.31%), Query Frame = 1


		  
Query: 101 GAAGGATTAAEEKVAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVV 160
           G+ G A+ + E K AEKT FDVKLEKF+A SK+KVIKE+RA T LGLKEAK LVEKAPV+
Sbjct: 81  GSVGDASASTETKTAEKTTFDVKLEKFEAASKIKVIKEIRAFTDLGLKEAKALVEKAPVI 140

Query: 161 LKQGLTKEEAEGMIEKIKAAGGSAVME 188
           +K GLTKEEAE ++EK+KA G    +E
Sbjct: 141 VKNGLTKEEAEKIMEKLKAVGAIVALE 167

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BLAST of Spo19253.1 vs. TAIR (Arabidopsis)
Match: AT4G36420.1 (Ribosomal protein L12 family protein)

HSP 1 Score: 102.1 bits (253), Expect = 3.900e-22
Identity = 64/145 (44.14%), Postives = 90/145 (62.07%), Query Frame = 1


		  
Query: 48  YGPRPPPSEKVTQLAEQIITLTSEERAQIGPALRELLKHPKLEKIST--EGLDLGGAAGG 107
           Y      SE V+++  ++  LT  E   +   LR+ L   +L  ++    G+ L G+   
Sbjct: 35  YSSPATQSENVSKIVNELSNLTLLETMDLTEILRQKLNISELPVMAAMMPGMSLPGSGAS 94

Query: 108 ATTAAE---EKVAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPVVLK 167
            +   E   +K   KTAFDV L+ +DA SK+KVIKEVR +T+LGLKEAK+LVEKAP +LK
Sbjct: 95  KSAGGEGKEKKKEAKTAFDVMLQAYDAVSKIKVIKEVRTITALGLKEAKDLVEKAPTLLK 154

Query: 168 QGLTKEEAEGMIEKIKAAGGSAVME 188
           +G++KEEAE +IEK+KA G    ME
Sbjct: 155 KGVSKEEAEKIIEKLKAVGAKVAME 179

[Close]

BLAST of Spo19253.1 vs. TAIR (Arabidopsis)
Match: AT2G03130.1 (Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family protein)

HSP 1 Score: 84.7 bits (208), Expect = 6.500e-17
Identity = 48/88 (54.55%), Postives = 64/88 (72.73%), Query Frame = 1


		  
Query: 100 GGAAGGATTAAEEKVAEKTAFDVKLEKFDATSKLKVIKEVRALTSLGLKEAKELVEKAPV 159
           G + GGA   A+   AEKT F+VKLE F+A +K+K+IKEVR +  +GLKEA +LV KAP 
Sbjct: 7   GQSGGGANEGAK---AEKTVFEVKLESFEARAKIKIIKEVRRIIGVGLKEAMKLVVKAPT 66

Query: 160 VLKQGLTKEEAEGMIEKIKAAGGSAVME 188
           VLK GL+KEE E ++EK+ A G  AV++
Sbjct: 67  VLKTGLSKEEGEEVVEKLNALGAEAVLQ 91

The following BLAST results are available for this feature:

BLAST of Spo19253.1 vs. NCBI nr
BLAST of Spo19253.1 vs. UniProtKB/TrEMBL
BLAST of Spo19253.1 vs. ExPASy Swiss-Prot
BLAST of Spo19253.1 vs. TAIR (Arabidopsis)

BLAST of Spo19253.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902239814\|gb\|KNA25741.1\|	8.1e-94	100.	hypothetical protein SOVF_0040...	[more]
gi\|731335536\|ref\|XP_010678774.1\|	1.6e-73	77.7	PREDICTED: 50S ribosomal prote...	[more]
gi\|702325563\|ref\|XP_010053490.1\|	3.0e-56	71.8	PREDICTED: 50S ribosomal prote...	[more]
gi\|697182448\|ref\|XP_009600228.1\|	1.3e-54	63.9	PREDICTED: 50S ribosomal prote...	[more]
gi\|565348179\|ref\|XP_006341095.1\|	3.7e-54	64.4	PREDICTED: 50S ribosomal prote...	[more]

BLAST of Spo19253.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9S1W9_SPIOL	5.6e-94	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8F4B8_BETVU	1.1e-73	77.7	Uncharacterized protein OS=Bet...	[more]
A0A059CHD8_EUCGR	2.1e-56	71.8	Uncharacterized protein OS=Euc...	[more]
A0A0V0HUT6_SOLCH	3.0e-55	64.9	Putative 54S ribosomal protein...	[more]
M1AHC8_SOLTU	2.5e-54	64.4	Uncharacterized protein OS=Sol...	[more]

BLAST of Spo19253.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
RK12_PHATC	5.7e-15	42.2	50S ribosomal protein L12, chl...	[more]
RK12_THAPS	7.4e-15	41.3	50S ribosomal protein L12, chl...	[more]
RL7_PROM4	9.7e-15	38.0	50S ribosomal protein L7/L12 O...	[more]
RL7_LIBAF	1.3e-14	41.0	50S ribosomal protein L7/L12 O...	[more]
RK12_ODOSI	2.2e-14	40.1	50S ribosomal protein L12, chl...	[more]

BLAST of Spo19253.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G06040.1	1.7e-46	57.6	Ribosomal protein L12/ ATP-dep...	[more]
AT1G70190.1	8.4e-25	38.6	Ribosomal protein L7/L12, olig...	[more]
AT4G37660.1	1.2e-23	65.5	Ribosomal protein L12/ ATP-dep...	[more]
AT4G36420.1	3.9e-22	44.1	Ribosomal protein L12 family p...	[more]
AT2G03130.1	6.5e-17	54.5	Ribosomal protein L12/ ATP-dep...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013823	Ribosomal protein L7/L12, C-terminal	PRODOM	PD001326		coord: 121..178 score: 1.0
IPR013823	Ribosomal protein L7/L12, C-terminal	PFAM	PF00542	Ribosomal_L12	coord: 120..184 score: 9.0
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	GENE3D	3.30.1390.10		coord: 115..186 score: 1.9
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	unknown	SSF54736	ClpS-like	coord: 116..186 score: 1.92
None	No IPR available	PANTHER	PTHR24089	FAMILY NOT NAMED	coord: 24..187 score: 2.9
None	No IPR available	PANTHER	PTHR24089:SF314	SUBFAMILY NOT NAMED	coord: 24..187 score: 2.9

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034645	cellular macromolecule biosynthetic process
biological_process	GO:0009965	leaf morphogenesis
biological_process	GO:0006412	translation
biological_process	GO:0045038	protein import into chloroplast thylakoid membrane
biological_process	GO:0009306	protein secretion
biological_process	GO:0043953	protein transport by the Tat complex
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0042254	ribosome biogenesis
biological_process	GO:0015979	photosynthesis
biological_process	GO:0030154	cell differentiation
cellular_component	GO:0009654	photosystem II oxygen evolving complex
cellular_component	GO:0019898	extrinsic component of membrane
cellular_component	GO:0009543	chloroplast thylakoid lumen
cellular_component	GO:0033281	TAT protein transport complex
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0005840	ribosome
molecular_function	GO:0009977	proton motive force dependent protein transmembrane transporter activity
molecular_function	GO:0003735	structural constituent of ribosome
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0019843	rRNA binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo03139	0.71	Barchart \| Table
Spo04709	0.68	Barchart \| Table
Spo18887	0.68	Barchart \| Table
Spo26489	0.66	Barchart \| Table
Spo04460	0.65	Barchart \| Table