Homology
BLAST of Spo16696.1 vs. NCBI nr
Match:
gi|902157172|gb|KNA06099.1| (hypothetical protein SOVF_184210 [Spinacia oleracea])
HSP 1 Score: 1174.8 bits (3038), Expect = 0.000e+0
Identity = 567/570 (99.47%), Postives = 567/570 (99.47%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPETDGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVK 60
MDLGTSYQYPVAPGPE DGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVK
Sbjct: 1 MDLGTSYQYPVAPGPEADGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVK 60
Query: 61 YAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMI 120
YAMECRKE AKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMI
Sbjct: 61 YAMECRKE--AKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMI 120
Query: 121 INALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAK 180
INALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAK
Sbjct: 121 INALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAK 180
Query: 181 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHS 240
LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHS
Sbjct: 181 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHS 240
Query: 241 REAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDY 300
REAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDY
Sbjct: 241 REAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDY 300
Query: 301 IFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDN 360
IFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDN
Sbjct: 301 IFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDN 360
Query: 361 PQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEA 420
PQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEA
Sbjct: 361 PQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEA 420
Query: 421 SSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQ 480
SSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQ
Sbjct: 421 SSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQ 480
Query: 481 NWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQ 540
NWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQ
Sbjct: 481 NWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQ 540
Query: 541 CTATELDDFLTLDAYNHIVIQHPKPGMPRL 571
CTATELDDFLTLDAYNHIVIQHPKPGMPRL
Sbjct: 541 CTATELDDFLTLDAYNHIVIQHPKPGMPRL 568
BLAST of Spo16696.1 vs. NCBI nr
Match:
gi|731333839|ref|XP_010677928.1| (PREDICTED: malate synthase, glyoxysomal [Beta vulgaris subsp. vulgaris])
HSP 1 Score: 1027.3 bits (2655), Expect = 1.000e-296
Identity = 492/570 (86.32%), Postives = 532/570 (93.33%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPETDGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVK 60
MDLG++Y +P A +D YEVP+GVDVRGRYD E+AKIL+KDALQFVAELQREF++H+K
Sbjct: 1 MDLGSTYHFP-AVRRSSDSYEVPEGVDVRGRYDEEYAKILTKDALQFVAELQREFRNHIK 60
Query: 61 YAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMI 120
YAM+CRKE AKAKYNSGALP FDPTT+KIREG+W C+APPPAVADRRVEITGPVERKMI
Sbjct: 61 YAMQCRKE--AKAKYNSGALPWFDPTTRKIREGNWVCSAPPPAVADRRVEITGPVERKMI 120
Query: 121 INALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAK 180
INALNSGAKVFMADFEDALSPSWENL+KGQVNLR+AVNGTISFH+K RNK YKLNDQ AK
Sbjct: 121 INALNSGAKVFMADFEDALSPSWENLIKGQVNLREAVNGTISFHDKTRNKIYKLNDQTAK 180
Query: 181 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHS 240
LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYF+HNFA FR+ GDGFGPFFYLPK+EHS
Sbjct: 181 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFHHNFAKFRQTHGDGFGPFFYLPKMEHS 240
Query: 241 REAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDY 300
EAKIW NVFEKAEK+AGIE GSIRATVLIETLPAVFQMNEILYELRDHS GLNCGRWDY
Sbjct: 241 MEAKIWKNVFEKAEKMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSIGLNCGRWDY 300
Query: 301 IFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDN 360
IFSYIKTFQSHPDRL+PDRVQVGM+QHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRD+
Sbjct: 301 IFSYIKTFQSHPDRLLPDRVQVGMTQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDD 360
Query: 361 PQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEA 420
+AN+ A+ELVK+DKLREV AGHDGTWAAHPGLIPPILE FN NMG+APNQI SITRPEA
Sbjct: 361 AEANKAALELVKLDKLREVNAGHDGTWAAHPGLIPPILEVFNANMGDAPNQIRSITRPEA 420
Query: 421 SSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQ 480
S+ISE+DLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISR Q
Sbjct: 421 STISEDDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRTQ 480
Query: 481 NWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQ 540
NWQWLK++AELDGDG G++V PELFARVVEEEM+RIQKEVG+++FK YKEAC IFT Q
Sbjct: 481 NWQWLKYQAELDGDGFGIRVNPELFARVVEEEMERIQKEVGNERFKKGMYKEACKIFTNQ 540
Query: 541 CTATELDDFLTLDAYNHIVIQHPKPGMPRL 571
CTATELDDFLTLDAYNHIVI HPK G+PRL
Sbjct: 541 CTATELDDFLTLDAYNHIVIHHPK-GLPRL 566
BLAST of Spo16696.1 vs. NCBI nr
Match:
gi|1011997783|ref|XP_015965844.1| (PREDICTED: malate synthase, glyoxysomal [Arachis duranensis])
HSP 1 Score: 958.4 bits (2476), Expect = 5.700e-276
Identity = 459/565 (81.24%), Postives = 506/565 (89.56%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPETD-GYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHV 60
MD GT Y YP + D Y+VP+GVD+RGRYD EFAKIL+KDAL+FVAELQREF+SH+
Sbjct: 1 MDTGT-YGYPKQGVKKIDPSYDVPEGVDIRGRYDEEFAKILTKDALKFVAELQREFRSHI 60
Query: 61 KYAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKM 120
+YA+ECRKEAK + YN GALPGFDP T+ IREG+W CA PPAVADR+VEITGPVERKM
Sbjct: 61 RYALECRKEAKRR--YNEGALPGFDPATRYIREGEWVCAPVPPAVADRKVEITGPVERKM 120
Query: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIA 180
+INALNSGAKVFMADFEDALSPSWENLM+GQVNL+DAV GTI+F++KARNK YKLNDQ A
Sbjct: 121 VINALNSGAKVFMADFEDALSPSWENLMRGQVNLKDAVAGTITFNDKARNKVYKLNDQTA 180
Query: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEH 240
KLFVRPRGWHLPEAHI IDGEPATGCLVDFGLYFYHN A FR+ QG GFGPFFYLPK+EH
Sbjct: 181 KLFVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFYHNHAPFRQTQGAGFGPFFYLPKMEH 240
Query: 241 SREAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWD 300
SREAKIWNNVFE+AEK+AGIE GSIRATVLIETLPAVFQMNEILYELRDHS GLNCGRWD
Sbjct: 241 SREAKIWNNVFERAEKMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWD 300
Query: 301 YIFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRD 360
YIFSY+KTFQ+HPDRL+PDRV VGM+QHFM+SYSDLLI TCHRRGVHAMGGMAAQIPIRD
Sbjct: 301 YIFSYVKTFQAHPDRLLPDRVLVGMTQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIRD 360
Query: 361 NPQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPE 420
+P ANE A+ELV+ DKLREVKAGHDGTWAAHPGLIP +E FN NMGNAPNQI R +
Sbjct: 361 DPAANEAALELVRKDKLREVKAGHDGTWAAHPGLIPACVEVFNNNMGNAPNQIKDAKRDD 420
Query: 421 ASSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRA 480
A+SI+EEDLLQ+PRGVRTM+GLRLNTRVGIQY+AAWLTGSG+VPLYNLMEDAATAEISR
Sbjct: 421 AASITEEDLLQIPRGVRTMDGLRLNTRVGIQYVAAWLTGSGSVPLYNLMEDAATAEISRV 480
Query: 481 QNWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTR 540
QNWQW+K+ ELDGDGLGV+V+ ELF+RVVEEEM RI+ EVG DKFK YKEAC IF R
Sbjct: 481 QNWQWIKYGVELDGDGLGVRVSKELFSRVVEEEMARIESEVGKDKFKKGMYKEACKIFKR 540
Query: 541 QCTATELDDFLTLDAYNHIVIQHPK 565
QCTA LDDFLTLDAYN+IVIQHPK
Sbjct: 541 QCTAPTLDDFLTLDAYNYIVIQHPK 562
BLAST of Spo16696.1 vs. NCBI nr
Match:
gi|922333458|ref|XP_013444720.1| (glyoxysomal malate synthase [Medicago truncatula])
HSP 1 Score: 957.2 bits (2473), Expect = 1.300e-275
Identity = 456/564 (80.85%), Postives = 503/564 (89.18%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPET-DGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHV 60
MDLGT Y +P Y+ P+GV+VRGRYD EFAKIL+KDAL+FVA+LQREF++H+
Sbjct: 1 MDLGT-YGFPTPTAKNIISNYDAPEGVEVRGRYDAEFAKILTKDALKFVADLQREFRNHI 60
Query: 61 KYAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKM 120
KYA+ECRKEAK + YN GALPGFDP T+ IREGDW CA PPAV+DR+VEITGPVERKM
Sbjct: 61 KYALECRKEAKRR--YNEGALPGFDPATRYIREGDWICAPVPPAVSDRKVEITGPVERKM 120
Query: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIA 180
IINALNSGAKVFMADFEDALSPSWENLMKGQVNL+DAV GTI+FH+K RNK YKLND+IA
Sbjct: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLKDAVAGTITFHDKGRNKVYKLNDEIA 180
Query: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEH 240
KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHN + FR QG GFGPFFYLPK+EH
Sbjct: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNHSTFRRSQGGGFGPFFYLPKMEH 240
Query: 241 SREAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWD 300
SREAKIWN VFEKAEK+ GIE GSIRATVLIETLPAVFQM+EILYELRDHS GLNCGRWD
Sbjct: 241 SREAKIWNKVFEKAEKMTGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWD 300
Query: 301 YIFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRD 360
YIFSY+KTF +HPDRL+PDRVQVGMSQHFM+SYSDLLI TCHRRGVHAMGGMAAQIPIRD
Sbjct: 301 YIFSYVKTFHAHPDRLLPDRVQVGMSQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIRD 360
Query: 361 NPQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPE 420
+P ANE A+ELV+ DKLREVKAGHDGTWAAHPGLIP +E FN NMGNAPNQI ++ R +
Sbjct: 361 DPVANEAALELVRKDKLREVKAGHDGTWAAHPGLIPSCMEIFNNNMGNAPNQITTMKRED 420
Query: 421 ASSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRA 480
A+ ++EEDLLQ+PRGVRTMEGLRLNTRVGIQY+AAWLTGSG+VPLYNLMEDAATAEISR
Sbjct: 421 AAKLTEEDLLQIPRGVRTMEGLRLNTRVGIQYVAAWLTGSGSVPLYNLMEDAATAEISRV 480
Query: 481 QNWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTR 540
QNWQWLK+ ELDGDGLGVKV+ ELF RVVEEEMDRI+KEVG DKFK YK+AC IFTR
Sbjct: 481 QNWQWLKYGVELDGDGLGVKVSKELFGRVVEEEMDRIEKEVGKDKFKKGMYKDACKIFTR 540
Query: 541 QCTATELDDFLTLDAYNHIVIQHP 564
QCT+ LDDFLTLDAYN+IV+ HP
Sbjct: 541 QCTSPTLDDFLTLDAYNYIVVVHP 561
BLAST of Spo16696.1 vs. NCBI nr
Match:
gi|217074760|gb|ACJ85740.1| (unknown [Medicago truncatula])
HSP 1 Score: 955.7 bits (2469), Expect = 3.700e-275
Identity = 455/564 (80.67%), Postives = 502/564 (89.01%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPET-DGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHV 60
MDLGT Y +P Y+ P+GV+VRGRYD EFAKIL+KDAL+FVA+LQREF++H+
Sbjct: 1 MDLGT-YGFPTPTAKNIISNYDAPEGVEVRGRYDAEFAKILTKDALKFVADLQREFRNHI 60
Query: 61 KYAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKM 120
KYA+ECRKEAK + YN GALPGFDP T+ IREGDW CA PPAV+DR+VEITGPVERKM
Sbjct: 61 KYALECRKEAKRR--YNEGALPGFDPATRYIREGDWICAPVPPAVSDRKVEITGPVERKM 120
Query: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIA 180
IINALNSGAKVFMADFEDALSPSWENLMKGQVNL+DAV GTI+FH+K RNK YKLND+IA
Sbjct: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLKDAVAGTITFHDKGRNKVYKLNDEIA 180
Query: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEH 240
KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHN + FR QG GFGPFFYLPK+EH
Sbjct: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNHSTFRRSQGGGFGPFFYLPKMEH 240
Query: 241 SREAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWD 300
SREAKIWN VFEKAEK+ GIE GSIRATVLIETLPAVFQM+EILYELRDHS GLNCGRWD
Sbjct: 241 SREAKIWNKVFEKAEKMTGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWD 300
Query: 301 YIFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRD 360
YIFSY+KTF +HPDRL+PDRVQVGMSQHFM+SYSDLLI TCHRRGVHAMGGMAAQIPIRD
Sbjct: 301 YIFSYVKTFHAHPDRLLPDRVQVGMSQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIRD 360
Query: 361 NPQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPE 420
+P ANE A+EL + DKLREVKAGHDGTWAAHPGLIP +E FN NMGNAPNQI ++ R +
Sbjct: 361 DPVANEAALELARKDKLREVKAGHDGTWAAHPGLIPSCMEIFNNNMGNAPNQITTMKRED 420
Query: 421 ASSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRA 480
A+ ++EEDLLQ+PRGVRTMEGLRLNTRVGIQY+AAWLTGSG+VPLYNLMEDAATAEISR
Sbjct: 421 AAKLTEEDLLQIPRGVRTMEGLRLNTRVGIQYVAAWLTGSGSVPLYNLMEDAATAEISRV 480
Query: 481 QNWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTR 540
QNWQWLK+ ELDGDGLGVKV+ ELF RVVEEEMDRI+KEVG DKFK YK+AC IFTR
Sbjct: 481 QNWQWLKYGVELDGDGLGVKVSKELFGRVVEEEMDRIEKEVGKDKFKKGMYKDACKIFTR 540
Query: 541 QCTATELDDFLTLDAYNHIVIQHP 564
QCT+ LDDFLTLDAYN+IV+ HP
Sbjct: 541 QCTSPTLDDFLTLDAYNYIVVVHP 561
BLAST of Spo16696.1 vs. UniProtKB/TrEMBL
Match:
A0A0K9QFS2_SPIOL (Malate synthase OS=Spinacia oleracea GN=SOVF_184210 PE=3 SV=1)
HSP 1 Score: 1174.8 bits (3038), Expect = 0.000e+0
Identity = 567/570 (99.47%), Postives = 567/570 (99.47%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPETDGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVK 60
MDLGTSYQYPVAPGPE DGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVK
Sbjct: 1 MDLGTSYQYPVAPGPEADGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVK 60
Query: 61 YAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMI 120
YAMECRKE AKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMI
Sbjct: 61 YAMECRKE--AKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMI 120
Query: 121 INALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAK 180
INALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAK
Sbjct: 121 INALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAK 180
Query: 181 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHS 240
LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHS
Sbjct: 181 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHS 240
Query: 241 REAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDY 300
REAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDY
Sbjct: 241 REAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDY 300
Query: 301 IFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDN 360
IFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDN
Sbjct: 301 IFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDN 360
Query: 361 PQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEA 420
PQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEA
Sbjct: 361 PQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEA 420
Query: 421 SSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQ 480
SSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQ
Sbjct: 421 SSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQ 480
Query: 481 NWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQ 540
NWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQ
Sbjct: 481 NWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQ 540
Query: 541 CTATELDDFLTLDAYNHIVIQHPKPGMPRL 571
CTATELDDFLTLDAYNHIVIQHPKPGMPRL
Sbjct: 541 CTATELDDFLTLDAYNHIVIQHPKPGMPRL 568
BLAST of Spo16696.1 vs. UniProtKB/TrEMBL
Match:
A0A0J8CGS7_BETVU (Malate synthase OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g110660 PE=3 SV=1)
HSP 1 Score: 1027.3 bits (2655), Expect = 7.000e-297
Identity = 492/570 (86.32%), Postives = 532/570 (93.33%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPETDGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVK 60
MDLG++Y +P A +D YEVP+GVDVRGRYD E+AKIL+KDALQFVAELQREF++H+K
Sbjct: 1 MDLGSTYHFP-AVRRSSDSYEVPEGVDVRGRYDEEYAKILTKDALQFVAELQREFRNHIK 60
Query: 61 YAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMI 120
YAM+CRKE AKAKYNSGALP FDPTT+KIREG+W C+APPPAVADRRVEITGPVERKMI
Sbjct: 61 YAMQCRKE--AKAKYNSGALPWFDPTTRKIREGNWVCSAPPPAVADRRVEITGPVERKMI 120
Query: 121 INALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAK 180
INALNSGAKVFMADFEDALSPSWENL+KGQVNLR+AVNGTISFH+K RNK YKLNDQ AK
Sbjct: 121 INALNSGAKVFMADFEDALSPSWENLIKGQVNLREAVNGTISFHDKTRNKIYKLNDQTAK 180
Query: 181 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHS 240
LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYF+HNFA FR+ GDGFGPFFYLPK+EHS
Sbjct: 181 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFHHNFAKFRQTHGDGFGPFFYLPKMEHS 240
Query: 241 REAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDY 300
EAKIW NVFEKAEK+AGIE GSIRATVLIETLPAVFQMNEILYELRDHS GLNCGRWDY
Sbjct: 241 MEAKIWKNVFEKAEKMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSIGLNCGRWDY 300
Query: 301 IFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDN 360
IFSYIKTFQSHPDRL+PDRVQVGM+QHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRD+
Sbjct: 301 IFSYIKTFQSHPDRLLPDRVQVGMTQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDD 360
Query: 361 PQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEA 420
+AN+ A+ELVK+DKLREV AGHDGTWAAHPGLIPPILE FN NMG+APNQI SITRPEA
Sbjct: 361 AEANKAALELVKLDKLREVNAGHDGTWAAHPGLIPPILEVFNANMGDAPNQIRSITRPEA 420
Query: 421 SSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQ 480
S+ISE+DLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISR Q
Sbjct: 421 STISEDDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRTQ 480
Query: 481 NWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQ 540
NWQWLK++AELDGDG G++V PELFARVVEEEM+RIQKEVG+++FK YKEAC IFT Q
Sbjct: 481 NWQWLKYQAELDGDGFGIRVNPELFARVVEEEMERIQKEVGNERFKKGMYKEACKIFTNQ 540
Query: 541 CTATELDDFLTLDAYNHIVIQHPKPGMPRL 571
CTATELDDFLTLDAYNHIVI HPK G+PRL
Sbjct: 541 CTATELDDFLTLDAYNHIVIHHPK-GLPRL 566
BLAST of Spo16696.1 vs. UniProtKB/TrEMBL
Match:
G7ZYR8_MEDTR (Malate synthase OS=Medicago truncatula GN=MTR_8g028225 PE=3 SV=1)
HSP 1 Score: 957.2 bits (2473), Expect = 8.900e-276
Identity = 456/564 (80.85%), Postives = 503/564 (89.18%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPET-DGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHV 60
MDLGT Y +P Y+ P+GV+VRGRYD EFAKIL+KDAL+FVA+LQREF++H+
Sbjct: 1 MDLGT-YGFPTPTAKNIISNYDAPEGVEVRGRYDAEFAKILTKDALKFVADLQREFRNHI 60
Query: 61 KYAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKM 120
KYA+ECRKEAK + YN GALPGFDP T+ IREGDW CA PPAV+DR+VEITGPVERKM
Sbjct: 61 KYALECRKEAKRR--YNEGALPGFDPATRYIREGDWICAPVPPAVSDRKVEITGPVERKM 120
Query: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIA 180
IINALNSGAKVFMADFEDALSPSWENLMKGQVNL+DAV GTI+FH+K RNK YKLND+IA
Sbjct: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLKDAVAGTITFHDKGRNKVYKLNDEIA 180
Query: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEH 240
KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHN + FR QG GFGPFFYLPK+EH
Sbjct: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNHSTFRRSQGGGFGPFFYLPKMEH 240
Query: 241 SREAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWD 300
SREAKIWN VFEKAEK+ GIE GSIRATVLIETLPAVFQM+EILYELRDHS GLNCGRWD
Sbjct: 241 SREAKIWNKVFEKAEKMTGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWD 300
Query: 301 YIFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRD 360
YIFSY+KTF +HPDRL+PDRVQVGMSQHFM+SYSDLLI TCHRRGVHAMGGMAAQIPIRD
Sbjct: 301 YIFSYVKTFHAHPDRLLPDRVQVGMSQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIRD 360
Query: 361 NPQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPE 420
+P ANE A+ELV+ DKLREVKAGHDGTWAAHPGLIP +E FN NMGNAPNQI ++ R +
Sbjct: 361 DPVANEAALELVRKDKLREVKAGHDGTWAAHPGLIPSCMEIFNNNMGNAPNQITTMKRED 420
Query: 421 ASSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRA 480
A+ ++EEDLLQ+PRGVRTMEGLRLNTRVGIQY+AAWLTGSG+VPLYNLMEDAATAEISR
Sbjct: 421 AAKLTEEDLLQIPRGVRTMEGLRLNTRVGIQYVAAWLTGSGSVPLYNLMEDAATAEISRV 480
Query: 481 QNWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTR 540
QNWQWLK+ ELDGDGLGVKV+ ELF RVVEEEMDRI+KEVG DKFK YK+AC IFTR
Sbjct: 481 QNWQWLKYGVELDGDGLGVKVSKELFGRVVEEEMDRIEKEVGKDKFKKGMYKDACKIFTR 540
Query: 541 QCTATELDDFLTLDAYNHIVIQHP 564
QCT+ LDDFLTLDAYN+IV+ HP
Sbjct: 541 QCTSPTLDDFLTLDAYNYIVVVHP 561
BLAST of Spo16696.1 vs. UniProtKB/TrEMBL
Match:
B7FLV7_MEDTR (Malate synthase OS=Medicago truncatula PE=2 SV=1)
HSP 1 Score: 955.7 bits (2469), Expect = 2.600e-275
Identity = 455/564 (80.67%), Postives = 502/564 (89.01%), Query Frame = 1
Query: 1 MDLGTSYQYPVAPGPET-DGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHV 60
MDLGT Y +P Y+ P+GV+VRGRYD EFAKIL+KDAL+FVA+LQREF++H+
Sbjct: 1 MDLGT-YGFPTPTAKNIISNYDAPEGVEVRGRYDAEFAKILTKDALKFVADLQREFRNHI 60
Query: 61 KYAMECRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKM 120
KYA+ECRKEAK + YN GALPGFDP T+ IREGDW CA PPAV+DR+VEITGPVERKM
Sbjct: 61 KYALECRKEAKRR--YNEGALPGFDPATRYIREGDWICAPVPPAVSDRKVEITGPVERKM 120
Query: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIA 180
IINALNSGAKVFMADFEDALSPSWENLMKGQVNL+DAV GTI+FH+K RNK YKLND+IA
Sbjct: 121 IINALNSGAKVFMADFEDALSPSWENLMKGQVNLKDAVAGTITFHDKGRNKVYKLNDEIA 180
Query: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEH 240
KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHN + FR QG GFGPFFYLPK+EH
Sbjct: 181 KLFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNHSTFRRSQGGGFGPFFYLPKMEH 240
Query: 241 SREAKIWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWD 300
SREAKIWN VFEKAEK+ GIE GSIRATVLIETLPAVFQM+EILYELRDHS GLNCGRWD
Sbjct: 241 SREAKIWNKVFEKAEKMTGIERGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWD 300
Query: 301 YIFSYIKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRD 360
YIFSY+KTF +HPDRL+PDRVQVGMSQHFM+SYSDLLI TCHRRGVHAMGGMAAQIPIRD
Sbjct: 301 YIFSYVKTFHAHPDRLLPDRVQVGMSQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIRD 360
Query: 361 NPQANEKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPE 420
+P ANE A+EL + DKLREVKAGHDGTWAAHPGLIP +E FN NMGNAPNQI ++ R +
Sbjct: 361 DPVANEAALELARKDKLREVKAGHDGTWAAHPGLIPSCMEIFNNNMGNAPNQITTMKRED 420
Query: 421 ASSISEEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRA 480
A+ ++EEDLLQ+PRGVRTMEGLRLNTRVGIQY+AAWLTGSG+VPLYNLMEDAATAEISR
Sbjct: 421 AAKLTEEDLLQIPRGVRTMEGLRLNTRVGIQYVAAWLTGSGSVPLYNLMEDAATAEISRV 480
Query: 481 QNWQWLKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTR 540
QNWQWLK+ ELDGDGLGVKV+ ELF RVVEEEMDRI+KEVG DKFK YK+AC IFTR
Sbjct: 481 QNWQWLKYGVELDGDGLGVKVSKELFGRVVEEEMDRIEKEVGKDKFKKGMYKDACKIFTR 540
Query: 541 QCTATELDDFLTLDAYNHIVIQHP 564
QCT+ LDDFLTLDAYN+IV+ HP
Sbjct: 541 QCTSPTLDDFLTLDAYNYIVVVHP 561
BLAST of Spo16696.1 vs. UniProtKB/TrEMBL
Match:
A0A061FZB9_THECC (Malate synthase OS=Theobroma cacao GN=TCM_014591 PE=3 SV=1)
HSP 1 Score: 942.6 bits (2435), Expect = 2.300e-271
Identity = 447/565 (79.12%), Postives = 502/565 (88.85%), Query Frame = 1
Query: 6 SYQYPVAPGPETDGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVKYAMEC 65
+Y + + D Y+VP+GVD+RGR+D EFAKIL+KDALQFVA+LQREF++H+ YAM C
Sbjct: 6 NYGFTAPAARKIDAYDVPEGVDIRGRFDEEFAKILTKDALQFVADLQREFRNHINYAMGC 65
Query: 66 RKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMIINALN 125
RKEAK + YN GALPGFDP T+ IREG W CA PPAVADRRVEITGPVERKMIINALN
Sbjct: 66 RKEAKRR--YNEGALPGFDPATRYIREGQWTCAPVPPAVADRRVEITGPVERKMIINALN 125
Query: 126 SGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAKLFVRP 185
SGAKVFMADFEDALSPSWENLM+GQ+NL+DAV+GTI+FH++ARN+ YKLNDQIAKLFVRP
Sbjct: 126 SGAKVFMADFEDALSPSWENLMRGQINLKDAVDGTITFHDRARNRVYKLNDQIAKLFVRP 185
Query: 186 RGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHSREAKI 245
RGWHLPEAHILIDGEPATG LVDFGLYFYHN+A FR+ QG+GFGPFFYLPK+EHSREAKI
Sbjct: 186 RGWHLPEAHILIDGEPATGSLVDFGLYFYHNYAIFRKTQGEGFGPFFYLPKMEHSREAKI 245
Query: 246 WNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDYIFSYI 305
WN VFE+AEK+AGIE GSIRATVLIETLPAVFQM+EILYELRDHS GLNCGRWDYIFSY+
Sbjct: 246 WNCVFERAEKMAGIEKGSIRATVLIETLPAVFQMDEILYELRDHSVGLNCGRWDYIFSYV 305
Query: 306 KTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDNPQANE 365
KTFQ HPDRL+PDRVQVGM+QHFM+SYSDLLI TCHRRGVHAMGGMAAQIPIRD+P ANE
Sbjct: 306 KTFQGHPDRLLPDRVQVGMTQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPAANE 365
Query: 366 KAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEASSISE 425
A +LV+ DKLREVKAGHDGTWAAHPGLI +E F NMGNAPNQI ++ R +A++++E
Sbjct: 366 AAFDLVRKDKLREVKAGHDGTWAAHPGLIQACMEVFTNNMGNAPNQIQTVKREDAANLTE 425
Query: 426 EDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQNWQWL 485
EDLLQ PRGVRTMEGLRLNTRVGIQYLAAWLTG+G+VPLYNLMEDAATAEISR QNWQWL
Sbjct: 426 EDLLQRPRGVRTMEGLRLNTRVGIQYLAAWLTGAGSVPLYNLMEDAATAEISRVQNWQWL 485
Query: 486 KFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQCTATE 545
K+ ELDGDGLGV+V +LF RVVEEEM RI++EVG DKF+ YKEA IFTRQCTA
Sbjct: 486 KYGVELDGDGLGVRVNGDLFGRVVEEEMARIEREVGKDKFRKGMYKEAGKIFTRQCTAPT 545
Query: 546 LDDFLTLDAYNHIVIQHPKPGMPRL 571
LDDFLTLDAYNHIV+ HPK G+ +L
Sbjct: 546 LDDFLTLDAYNHIVVHHPK-GLSKL 567
BLAST of Spo16696.1 vs. ExPASy Swiss-Prot
Match:
MASY_CUCSA (Malate synthase, glyoxysomal OS=Cucumis sativus PE=2 SV=2)
HSP 1 Score: 937.6 bits (2422), Expect = 6.600e-272
Identity = 437/546 (80.04%), Postives = 494/546 (90.48%), Query Frame = 1
Query: 19 GYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVKYAMECRKEAKAKAKYNSG 78
GY+VP+GVD+RGRYD EFAKIL+K+AL F+A+LQR F++H+KY+MECR+EAK + YN G
Sbjct: 20 GYDVPEGVDIRGRYDEEFAKILNKEALLFIADLQRTFRNHIKYSMECRREAKRR--YNEG 79
Query: 79 ALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDA 138
LPGFDP TK IR+ +W CA PPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDA
Sbjct: 80 GLPGFDPATKYIRDSEWTCAPVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDA 139
Query: 139 LSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAKLFVRPRGWHLPEAHILID 198
LSP+WENLM+GQ+NL+DAV+GTISFH++ RN+ YKLND+ AKLFVRPRGWHLPEAHI ID
Sbjct: 140 LSPNWENLMRGQINLKDAVDGTISFHDRVRNRVYKLNDRTAKLFVRPRGWHLPEAHIFID 199
Query: 199 GEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHSREAKIWNNVFEKAEKIAG 258
GEPATGCLVDFGLYF+HN ANFR QG G+GPFFYLPK+EHSREAKIWN+VFE+AEK+AG
Sbjct: 200 GEPATGCLVDFGLYFFHNHANFRRSQGQGYGPFFYLPKMEHSREAKIWNSVFERAEKMAG 259
Query: 259 IENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDYIFSYIKTFQSHPDRLVPD 318
IE GSIRATVLIETLPAVFQMNEILYELRDHS GLNCGRWDYIFSY+KTFQ+HPDRL+PD
Sbjct: 260 IERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPD 319
Query: 319 RVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDNPQANEKAMELVKMDKLRE 378
RV VGM+QHFMRSYSDLLI TCHRRGVHAMGGMAAQIPIRD+P+ANE A+ELV+ DKLRE
Sbjct: 320 RVLVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPKANEVALELVRKDKLRE 379
Query: 379 VKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEASSISEEDLLQLPRGVRTM 438
VKAGHDGTWAAHPGLIP +E F NMGNAPNQI S+ R +A++++EEDLLQ PRGVRTM
Sbjct: 380 VKAGHDGTWAAHPGLIPACMEVFTNNMGNAPNQIRSMRRDDAANLTEEDLLQQPRGVRTM 439
Query: 439 EGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQNWQWLKFRAELDGDGLGV 498
EGLRLNTRVGIQYLAAWLTG+G+VPLYNL EDAATAEISR QNWQWLK+ ELDGDGLGV
Sbjct: 440 EGLRLNTRVGIQYLAAWLTGAGSVPLYNLAEDAATAEISRVQNWQWLKYGVELDGDGLGV 499
Query: 499 KVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQCTATELDDFLTLDAYNHI 558
+V ELF RVVEEEM+RI++EVG ++FK YKEAC +FTRQCTA LDDFLTLDAYN+I
Sbjct: 500 RVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKMFTRQCTAPNLDDFLTLDAYNYI 559
Query: 559 VIQHPK 565
VI HP+
Sbjct: 560 VIHHPR 563
BLAST of Spo16696.1 vs. ExPASy Swiss-Prot
Match:
MASY_RICCO (Malate synthase, glyoxysomal OS=Ricinus communis PE=2 SV=1)
HSP 1 Score: 935.3 bits (2416), Expect = 3.300e-271
Identity = 445/553 (80.47%), Postives = 501/553 (90.60%), Query Frame = 1
Query: 19 GYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVKYAMECRKEAKAKAKYNSG 78
GY+VP+GVD+RGRYD EFAKIL++DALQFVA+LQREF++ ++YA+ECRKEAK++ YN+G
Sbjct: 18 GYDVPEGVDIRGRYDGEFAKILTRDALQFVADLQREFRNRIRYAIECRKEAKSR--YNAG 77
Query: 79 ALPGFD-PTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMIINALNSGAKVFMADFED 138
ALPGF+ P TK IREG+W CA PPAVADR+VEITGPVERKMIINALNSGAKVFMADFED
Sbjct: 78 ALPGFEHPATKYIREGEWTCAPVPPAVADRKVEITGPVERKMIINALNSGAKVFMADFED 137
Query: 139 ALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAKLFVRPRGWHLPEAHILI 198
ALSPSWENLM+GQVNLRDAVNGTISFH+KARN+ YKLNDQIAKLFVRPRGWHLPEAHILI
Sbjct: 138 ALSPSWENLMRGQVNLRDAVNGTISFHDKARNRVYKLNDQIAKLFVRPRGWHLPEAHILI 197
Query: 199 DGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHSREAKIWNNVFEKAEKIA 258
DGEPATGCLVDFGLYFYHN+A FR QG G+GPFFYLPK+EHSREAKIWN VFEKAEK+A
Sbjct: 198 DGEPATGCLVDFGLYFYHNYAAFRRNQGAGYGPFFYLPKMEHSREAKIWNCVFEKAEKMA 257
Query: 259 GIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDYIFSYIKTFQSHPDRLVP 318
GIE GSIRATVLIETLPAVFQMNEILYELRDHS GLNCGRWDYIFSY+KTFQ+HPDR +P
Sbjct: 258 GIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRPLP 317
Query: 319 DRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDNPQANEKAMELVKMDKLR 378
DRVQVGM+QHFM+SYSDLL++TCHRRGVHAMGGMAAQIPIRD+P AN+ A+ELV+ DKLR
Sbjct: 318 DRVQVGMTQHFMKSYSDLLVWTCHRRGVHAMGGMAAQIPIRDDPAANKAALELVRKDKLR 377
Query: 379 EVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEASSISEEDLLQLPRGVRT 438
EVKAGHDGTWAAHPGLIP +E F NMGN P+QI ++ R +A++I+EEDL+Q PRGVRT
Sbjct: 378 EVKAGHDGTWAAHPGLIPACMEVFANNMGNTPHQIQAMKREDAANITEEDLIQRPRGVRT 437
Query: 439 MEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQNWQWLKFRAELDGDGLG 498
+EGLRLNTRVGIQYLAAWLTG+G+VPLYNLMEDAATAEISR QNWQWLK+ ELDGDGLG
Sbjct: 438 LEGLRLNTRVGIQYLAAWLTGTGSVPLYNLMEDAATAEISRVQNWQWLKYGVELDGDGLG 497
Query: 499 VKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQCTATELDDFLTLDAYNH 558
VKVT +L RVVE+EM RI++EVG +KFK YKEAC +F RQC A LDDFLTLDAYN+
Sbjct: 498 VKVTFDLLGRVVEDEMARIEREVGKEKFKKGMYKEACKMFVRQCAAPTLDDFLTLDAYNN 557
Query: 559 IVIQHPKPGMPRL 571
IVI +PK G RL
Sbjct: 558 IVIHYPK-GSSRL 567
BLAST of Spo16696.1 vs. ExPASy Swiss-Prot
Match:
MASY_ARATH (Malate synthase OS=Arabidopsis thaliana GN=MLS PE=1 SV=1)
HSP 1 Score: 934.1 bits (2413), Expect = 7.200e-271
Identity = 438/549 (79.78%), Postives = 498/549 (90.71%), Query Frame = 1
Query: 15 PETDGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVKYAMECRKEAKAKAK 74
P Y+ P GV+VRGRYD FAKIL+++AL FVAELQREF+ HVKYAMECR+EA+ +
Sbjct: 10 PNVAVYDSPDGVEVRGRYDQIFAKILTREALSFVAELQREFRGHVKYAMECRREARRR-- 69
Query: 75 YNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMIINALNSGAKVFMAD 134
YNSGA+PGFDP+TK IR+GDW CA+ PPAVADRRVEITGPVERKMIINALNSGAKVFMAD
Sbjct: 70 YNSGAVPGFDPSTKFIRDGDWSCASVPPAVADRRVEITGPVERKMIINALNSGAKVFMAD 129
Query: 135 FEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAKLFVRPRGWHLPEAH 194
FEDALSPSWENLM+G VNL+DAV+G+I+FH+K+RN+ YKLNDQ AKLFVRPRGWHLPEAH
Sbjct: 130 FEDALSPSWENLMRGHVNLKDAVDGSITFHDKSRNRVYKLNDQTAKLFVRPRGWHLPEAH 189
Query: 195 ILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHSREAKIWNNVFEKAE 254
ILIDGEPATGCLVDFGLYF+HN+A FR+ QG GFGPFFYLPK+EHSREAKIWN+VFE+AE
Sbjct: 190 ILIDGEPATGCLVDFGLYFFHNYAKFRQTQGSGFGPFFYLPKMEHSREAKIWNSVFERAE 249
Query: 255 KIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDYIFSYIKTFQSHPDR 314
K+AGIE GSIRATVLIETLPAVFQMNEILYELRDHS GLNCGRWDYIFSY+KTFQ+HPDR
Sbjct: 250 KMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDR 309
Query: 315 LVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDNPQANEKAMELVKMD 374
L+PDRV VGM QHFMRSYSDLLI TCH+RGVHAMGGMAAQIPIRD+P+ANE A++LV+ D
Sbjct: 310 LLPDRVLVGMGQHFMRSYSDLLIRTCHKRGVHAMGGMAAQIPIRDDPKANEMALDLVRKD 369
Query: 375 KLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEASSISEEDLLQLPRG 434
KLREV+AGHDGTWAAHPGLIP +EAF +MG +PNQI S+ R +A++I+EEDLLQ+PRG
Sbjct: 370 KLREVRAGHDGTWAAHPGLIPICMEAFTGHMGKSPNQIKSVKREDAAAITEEDLLQIPRG 429
Query: 435 VRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQNWQWLKFRAELDGD 494
VRT+EGLRLNTRVGIQYLAAWLTGSG+VPLYNLMEDAATAEISR QNWQW+++ ELDGD
Sbjct: 430 VRTLEGLRLNTRVGIQYLAAWLTGSGSVPLYNLMEDAATAEISRVQNWQWIRYGVELDGD 489
Query: 495 GLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQCTATELDDFLTLDA 554
GLGV+V+ ELF RVVEEEM+RI+KEVG DKFK+ YKEAC +FT+QCTA ELDDFLTL
Sbjct: 490 GLGVRVSKELFGRVVEEEMERIEKEVGKDKFKNGMYKEACKMFTKQCTAPELDDFLTLAV 549
Query: 555 YNHIVIQHP 564
YNHIV +P
Sbjct: 550 YNHIVAHYP 556
BLAST of Spo16696.1 vs. ExPASy Swiss-Prot
Match:
MASY_BRANA (Malate synthase, glyoxysomal OS=Brassica napus PE=2 SV=1)
HSP 1 Score: 931.0 bits (2405), Expect = 6.100e-270
Identity = 440/549 (80.15%), Postives = 499/549 (90.89%), Query Frame = 1
Query: 15 PETDGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVKYAMECRKEAKAKAK 74
P Y+ P GV+VRGRYD FAKIL++DAL FVAELQREF+ HV+YAMECR+E K +
Sbjct: 10 PNVAVYDSPDGVEVRGRYDQVFAKILTRDALGFVAELQREFRGHVRYAMECRREVKRR-- 69
Query: 75 YNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMIINALNSGAKVFMAD 134
YNSGA+PGFDP+TK IR+G+W CA+ PPAVADRRVEITGPVERKMIINALNSGAKVFMAD
Sbjct: 70 YNSGAVPGFDPSTKFIRDGEWVCASVPPAVADRRVEITGPVERKMIINALNSGAKVFMAD 129
Query: 135 FEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAKLFVRPRGWHLPEAH 194
FEDALSPSWENLM+GQVNL+DAV+G+I+F++KARNK YKLNDQ+AKLFVRPRGWHLPEAH
Sbjct: 130 FEDALSPSWENLMRGQVNLKDAVDGSITFNDKARNKVYKLNDQVAKLFVRPRGWHLPEAH 189
Query: 195 ILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHSREAKIWNNVFEKAE 254
ILIDGEPATGCLVDFGLYF+HN+A FR+ QG GFGPFFYLPK+EHSREAKIWN+VFE+AE
Sbjct: 190 ILIDGEPATGCLVDFGLYFFHNYAKFRQTQGSGFGPFFYLPKMEHSREAKIWNSVFERAE 249
Query: 255 KIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDYIFSYIKTFQSHPDR 314
K+AGIE GSIRATVLIETLPAVFQMNEILYELRDHS GLNCGRWDYIFSY+KTFQ+HPDR
Sbjct: 250 KMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDR 309
Query: 315 LVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDNPQANEKAMELVKMD 374
L+PDRV VGM QHFMRSYSDLLI TCH+RGVHAMGGMAAQIPIRD+P+ANE A++LVK D
Sbjct: 310 LLPDRVLVGMGQHFMRSYSDLLIRTCHKRGVHAMGGMAAQIPIRDDPKANEMALDLVKKD 369
Query: 375 KLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEASSISEEDLLQLPRG 434
KLREV+AGHDGTWAAHPGLIP ++AF+ +MGN PNQI S+ R +AS+I+EEDLLQ+PRG
Sbjct: 370 KLREVRAGHDGTWAAHPGLIPICMDAFS-HMGNNPNQIKSMKRDDASAITEEDLLQIPRG 429
Query: 435 VRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQNWQWLKFRAELDGD 494
VRT+EGLRLNTRVGIQYLAAWLTGSG+VPLYNLMEDAATAEISR QNWQW+++ ELDGD
Sbjct: 430 VRTLEGLRLNTRVGIQYLAAWLTGSGSVPLYNLMEDAATAEISRVQNWQWIRYGVELDGD 489
Query: 495 GLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQCTATELDDFLTLDA 554
GLGV+V+ ELF RVVEEEM+RI+KEVG DKFK YKEAC +FT+QCTA ELDDFLTL
Sbjct: 490 GLGVRVSKELFGRVVEEEMERIEKEVGKDKFKRGMYKEACKMFTKQCTAAELDDFLTLAV 549
Query: 555 YNHIVIQHP 564
Y+HIV +P
Sbjct: 550 YDHIVAHYP 555
BLAST of Spo16696.1 vs. ExPASy Swiss-Prot
Match:
MASY_SOYBN (Malate synthase, glyoxysomal (Fragment) OS=Glycine max PE=2 SV=1)
HSP 1 Score: 931.0 bits (2405), Expect = 6.100e-270
Identity = 440/560 (78.57%), Postives = 498/560 (88.93%), Query Frame = 1
Query: 6 SYQYPVAPGPETDGY-EVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVKYAME 65
+Y YP + + Y +VP+GVD+RGRYD EFAKIL+KDAL+FVA+LQREF++H+KYA+E
Sbjct: 2 TYGYPTPAVKKIESYYDVPEGVDIRGRYDAEFAKILTKDALKFVADLQREFRNHIKYALE 61
Query: 66 CRKEAKAKAKYNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMIINAL 125
CR+E AK KYN GALP FDP T IRE +W CA PPAVADR+VEITGPV+RKM+INAL
Sbjct: 62 CRRE--AKKKYNEGALPEFDPATTYIREQEWVCAPVPPAVADRKVEITGPVDRKMVINAL 121
Query: 126 NSGAKVFMADFEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAKLFVR 185
NSGAKVFMADFEDALSP WENLM+GQVNL+DAV GTIS H+KARN+ YKLNDQ AKLFVR
Sbjct: 122 NSGAKVFMADFEDALSPGWENLMRGQVNLKDAVAGTISLHDKARNRVYKLNDQTAKLFVR 181
Query: 186 PRGWHLPEAHILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHSREAK 245
PRGWHLPEAHILIDGEPATGCLVDFGLYFYH+++ FR QG GFGPFFYLPK+EHSREAK
Sbjct: 182 PRGWHLPEAHILIDGEPATGCLVDFGLYFYHSYSAFRRTQGAGFGPFFYLPKMEHSREAK 241
Query: 246 IWNNVFEKAEKIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDYIFSY 305
IWNNVFEKAEK+AGIE GSIRATVLIETLPAVFQMNEILYEL+DHS GLNCGRWDYIFSY
Sbjct: 242 IWNNVFEKAEKVAGIERGSIRATVLIETLPAVFQMNEILYELKDHSVGLNCGRWDYIFSY 301
Query: 306 IKTFQSHPDRLVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDNPQAN 365
+KTFQ+HPDRL+PDRV VGM+QHFM+SYSDLLI TCHRRGVHAMGGMAAQIPI+++P AN
Sbjct: 302 VKTFQAHPDRLLPDRVLVGMTQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIKEDPVAN 361
Query: 366 EKAMELVKMDKLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEASSIS 425
E A+ELV+ DKLREVKAGHDGTWAAHPGLIP +E FN NMGNA NQI ++ R + ++I+
Sbjct: 362 EVALELVRKDKLREVKAGHDGTWAAHPGLIPACMEIFNNNMGNASNQIDTVKREDGANIT 421
Query: 426 EEDLLQLPRGVRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQNWQW 485
E+DLLQ+PRG RTMEGLRLNTRVGIQY+AAWLTGSG+VPLYNLMEDAATAEISR QNWQW
Sbjct: 422 EQDLLQIPRGARTMEGLRLNTRVGIQYVAAWLTGSGSVPLYNLMEDAATAEISRVQNWQW 481
Query: 486 LKFRAELDGDGLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQCTAT 545
LK+ EL+GDGLGVKV ELF RVVEEEM RI+KEVG++KFK YKEAC IFTRQCT+
Sbjct: 482 LKYGVELNGDGLGVKVNKELFGRVVEEEMARIEKEVGTEKFKEGMYKEACKIFTRQCTSP 541
Query: 546 ELDDFLTLDAYNHIVIQHPK 565
LDDFLTLDAYN+IV+ HP+
Sbjct: 542 MLDDFLTLDAYNYIVVHHPR 559
BLAST of Spo16696.1 vs. TAIR (Arabidopsis)
Match:
AT5G03860.1 (malate synthase)
HSP 1 Score: 934.1 bits (2413), Expect = 4.100e-272
Identity = 438/549 (79.78%), Postives = 498/549 (90.71%), Query Frame = 1
Query: 15 PETDGYEVPQGVDVRGRYDHEFAKILSKDALQFVAELQREFKSHVKYAMECRKEAKAKAK 74
P Y+ P GV+VRGRYD FAKIL+++AL FVAELQREF+ HVKYAMECR+EA+ +
Sbjct: 10 PNVAVYDSPDGVEVRGRYDQIFAKILTREALSFVAELQREFRGHVKYAMECRREARRR-- 69
Query: 75 YNSGALPGFDPTTKKIREGDWCCAAPPPAVADRRVEITGPVERKMIINALNSGAKVFMAD 134
YNSGA+PGFDP+TK IR+GDW CA+ PPAVADRRVEITGPVERKMIINALNSGAKVFMAD
Sbjct: 70 YNSGAVPGFDPSTKFIRDGDWSCASVPPAVADRRVEITGPVERKMIINALNSGAKVFMAD 129
Query: 135 FEDALSPSWENLMKGQVNLRDAVNGTISFHEKARNKDYKLNDQIAKLFVRPRGWHLPEAH 194
FEDALSPSWENLM+G VNL+DAV+G+I+FH+K+RN+ YKLNDQ AKLFVRPRGWHLPEAH
Sbjct: 130 FEDALSPSWENLMRGHVNLKDAVDGSITFHDKSRNRVYKLNDQTAKLFVRPRGWHLPEAH 189
Query: 195 ILIDGEPATGCLVDFGLYFYHNFANFREKQGDGFGPFFYLPKIEHSREAKIWNNVFEKAE 254
ILIDGEPATGCLVDFGLYF+HN+A FR+ QG GFGPFFYLPK+EHSREAKIWN+VFE+AE
Sbjct: 190 ILIDGEPATGCLVDFGLYFFHNYAKFRQTQGSGFGPFFYLPKMEHSREAKIWNSVFERAE 249
Query: 255 KIAGIENGSIRATVLIETLPAVFQMNEILYELRDHSAGLNCGRWDYIFSYIKTFQSHPDR 314
K+AGIE GSIRATVLIETLPAVFQMNEILYELRDHS GLNCGRWDYIFSY+KTFQ+HPDR
Sbjct: 250 KMAGIERGSIRATVLIETLPAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDR 309
Query: 315 LVPDRVQVGMSQHFMRSYSDLLIYTCHRRGVHAMGGMAAQIPIRDNPQANEKAMELVKMD 374
L+PDRV VGM QHFMRSYSDLLI TCH+RGVHAMGGMAAQIPIRD+P+ANE A++LV+ D
Sbjct: 310 LLPDRVLVGMGQHFMRSYSDLLIRTCHKRGVHAMGGMAAQIPIRDDPKANEMALDLVRKD 369
Query: 375 KLREVKAGHDGTWAAHPGLIPPILEAFNPNMGNAPNQIWSITRPEASSISEEDLLQLPRG 434
KLREV+AGHDGTWAAHPGLIP +EAF +MG +PNQI S+ R +A++I+EEDLLQ+PRG
Sbjct: 370 KLREVRAGHDGTWAAHPGLIPICMEAFTGHMGKSPNQIKSVKREDAAAITEEDLLQIPRG 429
Query: 435 VRTMEGLRLNTRVGIQYLAAWLTGSGAVPLYNLMEDAATAEISRAQNWQWLKFRAELDGD 494
VRT+EGLRLNTRVGIQYLAAWLTGSG+VPLYNLMEDAATAEISR QNWQW+++ ELDGD
Sbjct: 430 VRTLEGLRLNTRVGIQYLAAWLTGSGSVPLYNLMEDAATAEISRVQNWQWIRYGVELDGD 489
Query: 495 GLGVKVTPELFARVVEEEMDRIQKEVGSDKFKSENYKEACTIFTRQCTATELDDFLTLDA 554
GLGV+V+ ELF RVVEEEM+RI+KEVG DKFK+ YKEAC +FT+QCTA ELDDFLTL
Sbjct: 490 GLGVRVSKELFGRVVEEEMERIEKEVGKDKFKNGMYKEACKMFTKQCTAPELDDFLTLAV 549
Query: 555 YNHIVIQHP 564
YNHIV +P
Sbjct: 550 YNHIVAHYP 556
The following BLAST results are available for this feature:
