Spo16558 (gene) | SpinachBase

Overview


Name	Spo16558
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	RNA pseudouridine synthase
Location	SpoScf_00596 : 18635 .. 21978 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATGTTCAAGAAGGAAATTCTTCTGAAATGTGTTAGCTCTGATACCAAAGTAACAGTGAGGTACTGTTAATGGCTGAATGAAGTCAGAACTTCAACAGGACTTGATCAAAGATAACATGACAGAAGAAATAGAGAGAATAGAGAGAATTTATTAGGAAGAAGGAGTTTATTATTAACAATCATAACTCATAAGTGCTGTAAGCTCTGAATACAGCTATTTATACAATAGCTGAGCTACCTTAGCTAACTTTCCCACCACAATTCAGTTATAACAACATAACAGATTTCCTAATTATAAGGAGAGGCAAATAACAGATTCTCCCGTTACAAAAGTTGCCAAATTTCTGCAATTAAATACAAAAACTGCATGCAGGATGAGGATTATGTATTTCCCTTTAACTTAAATCCTTGCTTTTAATTTTCAGTAATTATGCAAGTTCTCGAAAAAAAGAAAAATGCCTTTATGGATTTCATTGTTACTCAAATTTAGGGAGGTCTCAATTCTTGTAGCCATACGGCCTTACCCCTATTTATACAGTCATATTCAATCAAATTGCTAGAGTAGTGCAACAAATACAAGTTTCTATATATTAAGCTAAATATATGCGCCCGGTAATCGACACTTGCTGTTTCTTCTACTAGTTCTACATACCTAGTATTCTTGTTAATTATTTTAAGTGTTTATGGATGTGATGTTGAGATGCTATATAGGAGTAGCTTATCTTTTCTTAGCTTGTGATGGATAAAAAAGATTAGTGTAATTTGAAAGTTCTGCACATAAGCTTGTGTTAAAGTAAAGCTATGCAGTATTGCTATTAAGGTAAGTTTCACGTAGAGCTTAGCGTTTCATTGAATGCAGAATATGTGAAAGGATCTCAGTTATGTCAGCTTGAAGTTTTGGATTGCAAAGAAGTTCCTTGGCCAAACTCTATAATTGAAGAGAAGTATCAAATCAATGACTGCGGATGGCCTGCCAAGAATTTTGCTTATGAATGTAAAATCAATCTTTTGACTGGCCGAACTCATCAGGTATCATGCTCAACTCTATGTTACTCAGAGGCTTCATTTCCACTCAAGTACATGTGTCGGATCCTTGACACTTAGACATGGTTATGGATAAACACTTAATTTTGGACCGAAAACACGAATTTTGTTTGAGTGGTAGAGGAGCTGAAATCTAATGTCAATATCCGAGTTCGGTTTTCTTTGTTAATTTCTTTGGGAATTGAGATACAGAACTACAGAAGTAATAAGTCCTATTTTTCCAGCATCGTCAACAGTTTTGTATGTCCACTGTTTTTTCTGGAATGATATTTCCGCCTATGTGTACCGTCTGACATCTTGGAATGATACAAAACATGAATATTTGAACTTTTACCGTTTTCTATCACTTATACAAAACATGAATATTTGAACTTCTACCATTTTCTATCACTTATACAAAACATGAATATTTGAACTTCTACCATTTTCTATCACTTATACAAAACATGAATATTTGTTCCCATGAACATTCCTTTTCAGTTAGTTTACAAACATCTGGGCACAGAAGAAACTTGTTGATTTACTCGTGTCCCTAATTTTCTGGACAGATTCGAGCACAACTTGCTGCTTGTGGGGCTCCTTTAATAGGTGATTCAATGTACATGCCAGCCGCACTTGCAGAGTTAACAAATCCTGGAGTTTTTCCCTTTGGTAAATACAAAAGAGAATATTCACGTGAGGATGACAAAAGAATTGCTATTGAGAATTGGATATCTCTGCACGGAAAGGAGCCAAGTTTGGCTATCGGTCTTCAAGCAAGTCAAATATCATGGGATGATGATAAGTACGTTTATGAAGCTGGATCACCTTGGTGGAGATGAGGACATATTCAGGGGTCTGTTGCACTCGAAGAACTTAAAATTTCTTGTTGATGAAGCCGGGTTTGGCTGATGTTGGATTCCATTGTTTCTGGTCTTCAGTTTTCAAGGTTACATGTCAATGCTTCTGCATGCATGGTAAGCATTTCATTTATGTAAACATAATAAAGAAAAGATTTGTGTAAATAATCTGAAGTTTCATTTATTATCCCACTTTATGATTAGTTAGGTATAATCCAACTTATCCAAGATTTGGGGAACCTTCTCAAAATAACAGACCCTAAAAAATTCCAACATAGATGAGTCGATGACAATAATAGCACTTTGAACAGTTTGGAGTAAGTTTCCCCCAAAATTTGGGATTATCTGAAATAATCAAATGGAAGGATTTTTAAAAGAAAATGGTTAAAACCTGGGATTATTGATTTCAAGTTTTCTATTTCTCGAGAAAAAATTATCATCGTTCAATGGATTGGTAACCTCTCCGGATCCTTGTTCTTTTCACTACTTTTGGAAGCCGTTGTAGTATGTCGTCATGACTTAATCAGGCTTAATGTACTACAGTGCTGGATCTTTTCGTTTCATTGGAGCTGTTTTATCTCACGGGATTAATCAGTCTTAAATGTACTTACATGCTGAATCTTTTCGTTTCATTGGAGTTGTTTCTCTCACTGGATTCTGTTTCCTGATATTCTGTATTTACATCATTATTTCACACTATATCTTCAGGTAGGCTATAACGGCTGCTGTTTGTTTATTCTTCTCTACCTGTGGACCTGTTGTTGTGGAATGTTTGCATGCACTTATTGTTTGCCCCTAATCAAACAGCTTATGCTGTGAATGTGAAAAGTAGGAACGGTTAATTGTGGCATGCTGTCATTGGCTTTGGGGTTAATGATGTATCACGATGTATGATAATCTTACACCAATTAAGCTGTAAGCCTGTAACTTATCACGGATGGCTTTAAACAAGATCCAATGTTTTTCGTTCAAAATTGCTTTGTCTAAAAGTTTCTTTTCCGGGTTTTCCCCAGATTAGCAAACAGAAGATTGGATGCAGGAAGTTTCTATTACCTCGGAATTACATACTTGAAACAGAACTGTGAACTTCAAAGTCTCAAAACAAGTTACCAGAAAAGAGAACTCCTAATAAGACAGCTTAAGACCTGAAGACAAAGAGTGATATATAGCCTTTGTGGAAAAGTCTGTTCATTGCATCTTAACACGACATCAGGAAGGATTTTCAACTATACCTGATGTTTGTGTTTGGGTTTGTCAGAGGATGAGGTCAAGCTTCTGTATACTATTGTAACTGAAGGAAGTAATCTGTAAAGATTACAGATGTATTGTTTCAAGTAATTTGCTATAAGTTGACTTTGACTTTAATGTACTCCTACATTTTAAATTTACCTGTTATTTGAATTAATGATACTAACAGATGCCATAACTGTCTGTCTCACAAATCACCAATGCCGAA

mRNA sequence

ATGGAATATGTGAAAGGATCTCAGTTATGTCAGCTTGAAGTTTTGGATTGCAAAGAAGTTCCTTGGCCAAACTCTATAATTGAAGAGAAGTATCAAATCAATGACTGCGGATGGCCTGCCAAGAATTTTGCTTATGAATGTAAAATCAATCTTTTGACTGGCCGAACTCATCAGATTCGAGCACAACTTGCTGCTTGTGGGGCTCCTTTAATAGGTGATTCAATGTACATGCCAGCCGCACTTGCAGAGTTAACAAATCCTGGAGTTTTTCCCTTTGGTAAATACAAAAGAGAATATTCACGTGAGGATGACAAAAGAATTGCTATTGAGAATTGGATATCTCTGCACGGAAAGGAGCCAAGTTTGGCTATCGGTCTTCAAGCAAGTCAAATATCATGGGATGATGATAAGTACGTTTATGAAGCTGGATCACCTTGTTTTCAAGGTTACATGTCAATGCTTCTGCATGCATGATTAGCAAACAGAAGATTGGATGCAGGAAGTTTCTATTACCTCGGAATTACATACTTGAAACAGAACTGTGAACTTCAAAGTCTCAAAACAAGTTACCAGAAAAGAGAACTCCTAATAAGACAGCTTAAGACCTGAAGACAAAGAGTGATATATAGCCTTTGTGGAAAAGTCTGTTCATTGCATCTTAACACGACATCAGGAAGGATTTTCAACTATACCTGATGTTTGTGTTTGGGTTTGTCAGAGGATGAGGTCAAGCTTCTGTATACTATTGTAACTGAAGGAAGTAATCTGTAAAGATTACAGATGTATTGTTTCAAGTAATTTGCTATAAGTTGACTTTGACTTTAATGTACTCCTACATTTTAAATTTACCTGTTATTTGAATTAATGATACTAACAGATGCCATAACTGTCTGTCTCACAAATCACCAATGCCGAA

Coding sequence (CDS)

ATGGAATATGTGAAAGGATCTCAGTTATGTCAGCTTGAAGTTTTGGATTGCAAAGAAGTTCCTTGGCCAAACTCTATAATTGAAGAGAAGTATCAAATCAATGACTGCGGATGGCCTGCCAAGAATTTTGCTTATGAATGTAAAATCAATCTTTTGACTGGCCGAACTCATCAGATTCGAGCACAACTTGCTGCTTGTGGGGCTCCTTTAATAGGTGATTCAATGTACATGCCAGCCGCACTTGCAGAGTTAACAAATCCTGGAGTTTTTCCCTTTGGTAAATACAAAAGAGAATATTCACGTGAGGATGACAAAAGAATTGCTATTGAGAATTGGATATCTCTGCACGGAAAGGAGCCAAGTTTGGCTATCGGTCTTCAAGCAAGTCAAATATCATGGGATGATGATAAGTACGTTTATGAAGCTGGATCACCTTGTTTTCAAGGTTACATGTCAATGCTTCTGCATGCATGA

Protein sequence

MEYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRAQLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPSLAIGLQASQISWDDDKYVYEAGSPCFQGYMSMLLHA

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo16558.1	Spo16558.1	mRNA

Homology

[Close]

BLAST of Spo16558.1 vs. NCBI nr
Match: gi|902122870|gb|KNA04012.1| (hypothetical protein SOVF_203620 [Spinacia oleracea])

HSP 1 Score: 311.2 bits (796), Expect = 1.000e-81
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA
Sbjct: 317 EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 376

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS
Sbjct: 377 QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 436

Query: 122 LAIGLQASQISWDDDKYVYEAGSP 146
           LAIGLQASQISWDDDKYVYEAGSP
Sbjct: 437 LAIGLQASQISWDDDKYVYEAGSP 460

[Close]

BLAST of Spo16558.1 vs. NCBI nr
Match: gi|731369863|ref|XP_010665548.1| (PREDICTED: RNA pseudouridine synthase 6, chloroplastic-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 275.8 bits (704), Expect = 4.700e-71
Identity = 126/145 (86.90%), Postives = 135/145 (93.10%), Query Frame = 1


		  
Query: 1   MEYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIR 60
           +E+VKG QLCQLEVLDCK+VPWP+S IEEKYQINDCGWP KNFAYECKI+LLTGRTHQIR
Sbjct: 313 VEHVKGWQLCQLEVLDCKKVPWPSSTIEEKYQINDCGWPVKNFAYECKIDLLTGRTHQIR 372

Query: 61  AQLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEP 120
           AQLAACGAP+IGDSMYMPAALAELT PGV PFGK K E++R DDKR AI+NWISLHGKEP
Sbjct: 373 AQLAACGAPIIGDSMYMPAALAELTKPGVSPFGKCKAEHTRGDDKRFAIDNWISLHGKEP 432

Query: 121 SLAIGLQASQISWDDDKYVYEAGSP 146
           SLAIGLQA QISWDDDKYVYEAGSP
Sbjct: 433 SLAIGLQACQISWDDDKYVYEAGSP 457

[Close]

BLAST of Spo16558.1 vs. NCBI nr
Match: gi|743885907|ref|XP_011037691.1| (PREDICTED: RNA pseudouridine synthase 6, chloroplastic [Populus euphratica])

HSP 1 Score: 243.0 bits (619), Expect = 3.400e-61
Identity = 102/144 (70.83%), Postives = 126/144 (87.50%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           E++KG  LCQLEV++CK VPWP++++EEKY + DCGWP+K+ AYECKINLLTGRTHQIRA
Sbjct: 320 EFIKGWHLCQLEVMECKRVPWPDAVVEEKYCVQDCGWPSKDHAYECKINLLTGRTHQIRA 379

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           QLAA GAPL+GDSMYMPAA+AE+ +PG  PFGKYK++Y+ EDDK IA+E W+  HGKEP+
Sbjct: 380 QLAAVGAPLVGDSMYMPAAVAEMVSPGCNPFGKYKKQYTSEDDKAIAVEEWVLCHGKEPN 439

Query: 122 LAIGLQASQISWDDDKYVYEAGSP 146
           +AIGLQA QISWDD K++Y AGSP
Sbjct: 440 VAIGLQACQISWDDGKHIYNAGSP 463

[Close]

BLAST of Spo16558.1 vs. NCBI nr
Match: gi|566164431|ref|XP_002304926.2| (pseudouridine synthase family protein [Populus trichocarpa])

HSP 1 Score: 242.7 bits (618), Expect = 4.400e-61
Identity = 103/151 (68.21%), Postives = 130/151 (86.09%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           E++KG  LCQLEV++CK VPWP++++EEKY + DCGWP+K+ AYECKINLLTGRTHQIRA
Sbjct: 320 EFIKGWHLCQLEVMECKRVPWPDAVMEEKYCVQDCGWPSKDHAYECKINLLTGRTHQIRA 379

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           QLAA GAPL+GDSMYMPAA+AE+ +PG  PFGKYK++Y+ EDDK IA+E W+  HGKEP+
Sbjct: 380 QLAALGAPLVGDSMYMPAAVAEMVSPGCNPFGKYKKQYTSEDDKAIAVEEWVLCHGKEPN 439

Query: 122 LAIGLQASQISWDDDKYVYEAGSPCFQGYMS 153
           +AIGLQA QISWDD K++Y AGSP ++  M+
Sbjct: 440 VAIGLQACQISWDDGKHIYNAGSPWWRCRMA 470

[Close]

BLAST of Spo16558.1 vs. NCBI nr
Match: gi|223542359|gb|EEF43901.1| (ribosomal pseudouridine synthase, putative [Ricinus communis])

HSP 1 Score: 237.7 bits (605), Expect = 1.400e-59
Identity = 97/144 (67.36%), Postives = 126/144 (87.50%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           E+++G  LCQLEVL+C++VPWP+++ EEKY + DCGWP+K+FAYECKINLLTGRTHQIRA
Sbjct: 249 EFIEGWYLCQLEVLECRKVPWPDTVTEEKYSVEDCGWPSKDFAYECKINLLTGRTHQIRA 308

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           QLAACGAPL+GDSMYMPAA+AE+ NPGV PFGK+K++Y+ E+D+ + +  W+  HGKEPS
Sbjct: 309 QLAACGAPLVGDSMYMPAAIAEMANPGVNPFGKHKKQYTGENDESMGVAEWVERHGKEPS 368

Query: 122 LAIGLQASQISWDDDKYVYEAGSP 146
           +AIGLQA +ISW D K++Y+AG P
Sbjct: 369 IAIGLQACEISWGDSKHIYKAGPP 392

[Close]

BLAST of Spo16558.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9P9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_203620 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 7.100e-82
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA
Sbjct: 317 EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 376

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS
Sbjct: 377 QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 436

Query: 122 LAIGLQASQISWDDDKYVYEAGSP 146
           LAIGLQASQISWDDDKYVYEAGSP
Sbjct: 437 LAIGLQASQISWDDDKYVYEAGSP 460

[Close]

BLAST of Spo16558.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E0U7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g200350 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 3.300e-71
Identity = 126/145 (86.90%), Postives = 135/145 (93.10%), Query Frame = 1


		  
Query: 1   MEYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIR 60
           +E+VKG QLCQLEVLDCK+VPWP+S IEEKYQINDCGWP KNFAYECKI+LLTGRTHQIR
Sbjct: 313 VEHVKGWQLCQLEVLDCKKVPWPSSTIEEKYQINDCGWPVKNFAYECKIDLLTGRTHQIR 372

Query: 61  AQLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEP 120
           AQLAACGAP+IGDSMYMPAALAELT PGV PFGK K E++R DDKR AI+NWISLHGKEP
Sbjct: 373 AQLAACGAPIIGDSMYMPAALAELTKPGVSPFGKCKAEHTRGDDKRFAIDNWISLHGKEP 432

Query: 121 SLAIGLQASQISWDDDKYVYEAGSP 146
           SLAIGLQA QISWDDDKYVYEAGSP
Sbjct: 433 SLAIGLQACQISWDDDKYVYEAGSP 457

[Close]

BLAST of Spo16558.1 vs. UniProtKB/TrEMBL
Match: B9H1E8_POPTR (Pseudouridine synthase family protein OS=Populus trichocarpa GN=POPTR_0004s01870g PE=4 SV=2)

HSP 1 Score: 242.7 bits (618), Expect = 3.100e-61
Identity = 103/151 (68.21%), Postives = 130/151 (86.09%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           E++KG  LCQLEV++CK VPWP++++EEKY + DCGWP+K+ AYECKINLLTGRTHQIRA
Sbjct: 320 EFIKGWHLCQLEVMECKRVPWPDAVMEEKYCVQDCGWPSKDHAYECKINLLTGRTHQIRA 379

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           QLAA GAPL+GDSMYMPAA+AE+ +PG  PFGKYK++Y+ EDDK IA+E W+  HGKEP+
Sbjct: 380 QLAALGAPLVGDSMYMPAAVAEMVSPGCNPFGKYKKQYTSEDDKAIAVEEWVLCHGKEPN 439

Query: 122 LAIGLQASQISWDDDKYVYEAGSPCFQGYMS 153
           +AIGLQA QISWDD K++Y AGSP ++  M+
Sbjct: 440 VAIGLQACQISWDDGKHIYNAGSPWWRCRMA 470

[Close]

BLAST of Spo16558.1 vs. UniProtKB/TrEMBL
Match: B9RXP5_RICCO (Ribosomal pseudouridine synthase, putative OS=Ricinus communis GN=RCOM_0905340 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 9.900e-60
Identity = 97/144 (67.36%), Postives = 126/144 (87.50%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           E+++G  LCQLEVL+C++VPWP+++ EEKY + DCGWP+K+FAYECKINLLTGRTHQIRA
Sbjct: 249 EFIEGWYLCQLEVLECRKVPWPDTVTEEKYSVEDCGWPSKDFAYECKINLLTGRTHQIRA 308

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           QLAACGAPL+GDSMYMPAA+AE+ NPGV PFGK+K++Y+ E+D+ + +  W+  HGKEPS
Sbjct: 309 QLAACGAPLVGDSMYMPAAIAEMANPGVNPFGKHKKQYTGENDESMGVAEWVERHGKEPS 368

Query: 122 LAIGLQASQISWDDDKYVYEAGSP 146
           +AIGLQA +ISW D K++Y+AG P
Sbjct: 369 IAIGLQACEISWGDSKHIYKAGPP 392

[Close]

BLAST of Spo16558.1 vs. UniProtKB/TrEMBL
Match: A0A151U0Q0_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_005458 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.200e-59
Identity = 98/144 (68.06%), Postives = 127/144 (88.19%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           E++KG  LC+LEV++C++VPWP + +++KY I DCGWP++++AYECKINLLTGRTHQIRA
Sbjct: 326 EFIKGWHLCRLEVIECRKVPWPTTAVQDKYCIEDCGWPSQDYAYECKINLLTGRTHQIRA 385

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           Q AAC APLIGDSMYMPAA+A+++NPG+ PFGKYK+++S E +K  A+ NWIS HGKEPS
Sbjct: 386 QFAACRAPLIGDSMYMPAAIADISNPGLNPFGKYKKDFSSESEKETAVMNWISQHGKEPS 445

Query: 122 LAIGLQASQISWDDDKYVYEAGSP 146
           +AIGLQA QISWDDD++ Y+AGSP
Sbjct: 446 VAIGLQACQISWDDDEHFYQAGSP 469

[Close]

BLAST of Spo16558.1 vs. ExPASy Swiss-Prot
Match: PUS6_ARATH (RNA pseudouridine synthase 6, chloroplastic OS=Arabidopsis thaliana GN=At4g21770 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 3.400e-53
Identity = 92/145 (63.45%), Postives = 116/145 (80.00%), Query Frame = 1


		  
Query: 4   VKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRAQL 63
           ++G  LCQLEVL+CK++PWP++  E+K+ I DCGW +K FAYEC INLLTG+THQIRAQL
Sbjct: 325 IQGWHLCQLEVLECKKIPWPDAATEKKHDIEDCGWTSKEFAYECTINLLTGKTHQIRAQL 384

Query: 64  AACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDD-KRIAIENWISLHGKEPSL 123
           AACGAPL+GDSMYMPAA+AE+ NP   P+GK K+ Y+ E++ K  A+  WI  HGKEP +
Sbjct: 385 AACGAPLVGDSMYMPAAIAEMGNPDTNPYGKAKKHYTMEENQKETAVAEWIDRHGKEPRV 444

Query: 124 AIGLQASQISWDDD--KYVYEAGSP 146
            IGLQA QISWDDD  ++ YEAG+P
Sbjct: 445 GIGLQACQISWDDDDGEHFYEAGTP 469

[Close]

BLAST of Spo16558.1 vs. ExPASy Swiss-Prot
Match: PUS6_ORYSJ (RNA pseudouridine synthase 6, chloroplastic OS=Oryza sativa subsp. japonica GN=Os07g0660400 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 6.000e-50
Identity = 83/146 (56.85%), Postives = 114/146 (78.08%), Query Frame = 1


		  
Query: 2   EYVKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRA 61
           +++KG  +CQ+E+LDCK+VPWP+S+I + +++++CGWP +  AYECKINLLTG+THQIRA
Sbjct: 320 DHIKGWHVCQMEILDCKKVPWPSSLIRKVHKVDNCGWPQQEAAYECKINLLTGKTHQIRA 379

Query: 62  QLAACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDDKRIAIENWISLHGKEPS 121
           QLAA G P++GDS YM AA+A + NP + PFG++ + Y  ED+K  A+E WIS HGKEP 
Sbjct: 380 QLAAIGTPIVGDSAYMTAAMAAIVNPSINPFGRWGQNYDSEDEKAAAVEAWISCHGKEPK 439

Query: 122 LAIGLQASQISWD--DDKYVYEAGSP 146
             IGLQAS+ISWD   + + Y+AG P
Sbjct: 440 SVIGLQASEISWDYEGEHHSYKAGVP 465

[Close]

BLAST of Spo16558.1 vs. TAIR (Arabidopsis)
Match: AT4G21770.1 (Pseudouridine synthase family protein)

HSP 1 Score: 209.1 bits (531), Expect = 1.900e-54
Identity = 92/145 (63.45%), Postives = 116/145 (80.00%), Query Frame = 1


		  
Query: 4   VKGSQLCQLEVLDCKEVPWPNSIIEEKYQINDCGWPAKNFAYECKINLLTGRTHQIRAQL 63
           ++G  LCQLEVL+CK++PWP++  E+K+ I DCGW +K FAYEC INLLTG+THQIRAQL
Sbjct: 325 IQGWHLCQLEVLECKKIPWPDAATEKKHDIEDCGWTSKEFAYECTINLLTGKTHQIRAQL 384

Query: 64  AACGAPLIGDSMYMPAALAELTNPGVFPFGKYKREYSREDD-KRIAIENWISLHGKEPSL 123
           AACGAPL+GDSMYMPAA+AE+ NP   P+GK K+ Y+ E++ K  A+  WI  HGKEP +
Sbjct: 385 AACGAPLVGDSMYMPAAIAEMGNPDTNPYGKAKKHYTMEENQKETAVAEWIDRHGKEPRV 444

Query: 124 AIGLQASQISWDDD--KYVYEAGSP 146
            IGLQA QISWDDD  ++ YEAG+P
Sbjct: 445 GIGLQACQISWDDDDGEHFYEAGTP 469

The following BLAST results are available for this feature:

BLAST of Spo16558.1 vs. NCBI nr
BLAST of Spo16558.1 vs. UniProtKB/TrEMBL
BLAST of Spo16558.1 vs. ExPASy Swiss-Prot
BLAST of Spo16558.1 vs. TAIR (Arabidopsis)

BLAST of Spo16558.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902122870\|gb\|KNA04012.1\|	1.0e-81	100.	hypothetical protein SOVF_2036...	[more]
gi\|731369863\|ref\|XP_010665548.1\|	4.7e-71	86.9	PREDICTED: RNA pseudouridine s...	[more]
gi\|743885907\|ref\|XP_011037691.1\|	3.4e-61	70.8	PREDICTED: RNA pseudouridine s...	[more]
gi\|566164431\|ref\|XP_002304926.2\|	4.4e-61	68.2	pseudouridine synthase family ...	[more]
gi\|223542359\|gb\|EEF43901.1\|	1.4e-59	67.3	ribosomal pseudouridine syntha...	[more]

BLAST of Spo16558.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q9P9_SPIOL	7.1e-82	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8E0U7_BETVU	3.3e-71	86.9	Uncharacterized protein OS=Bet...	[more]
B9H1E8_POPTR	3.1e-61	68.2	Pseudouridine synthase family ...	[more]
B9RXP5_RICCO	9.9e-60	67.3	Ribosomal pseudouridine syntha...	[more]
A0A151U0Q0_CAJCA	2.2e-59	68.0	Uncharacterized protein OS=Caj...	[more]

BLAST of Spo16558.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2


Match Name	E-value	Identity	Description
PUS6_ARATH	3.4e-53	63.4	RNA pseudouridine synthase 6, ...	[more]
PUS6_ORYSJ	6.0e-50	56.8	RNA pseudouridine synthase 6, ...	[more]

BLAST of Spo16558.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G21770.1	1.9e-54	63.4	Pseudouridine synthase family ...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluB/C/D/E/F	PFAM	PF00849	PseudoU_synth_2	coord: 26..65 score: 2.
IPR020103	Pseudouridine synthase, catalytic domain	unknown	SSF55120	Pseudouridine synthase	coord: 46..83 score: 2.24
None	No IPR available	PANTHER	PTHR11079	CYTOSINE DEAMINASE	coord: 112..145 score: 1.6E-44coord: 1..94 score: 1.6
None	No IPR available	PANTHER	PTHR11079:SF79	RNA PSEUDOURIDINE SYNTHASE 6, CHLOROPLASTIC	coord: 112..145 score: 1.6E-44coord: 1..94 score: 1.6

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009099	valine biosynthetic process
biological_process	GO:0006544	glycine metabolic process
biological_process	GO:0006426	glycyl-tRNA aminoacylation
biological_process	GO:0006563	L-serine metabolic process
biological_process	GO:0006566	threonine metabolic process
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0006428	isoleucyl-tRNA aminoacylation
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0048481	plant ovule development
biological_process	GO:0006450	regulation of translational fidelity
biological_process	GO:0010027	thylakoid membrane organization
biological_process	GO:0010228	vegetative to reproductive phase transition of meristem
biological_process	GO:0006525	arginine metabolic process
biological_process	GO:0009902	chloroplast relocation
biological_process	GO:0016556	mRNA modification
biological_process	GO:0048255	mRNA stabilization
biological_process	GO:0034660	ncRNA metabolic process
biological_process	GO:0042793	plastid transcription
biological_process	GO:0010103	stomatal complex morphogenesis
biological_process	GO:0019750	chloroplast localization
biological_process	GO:0008152	metabolic process
biological_process	GO:0006606	protein import into nucleus
biological_process	GO:0045036	protein targeting to chloroplast
biological_process	GO:0006626	protein targeting to mitochondrion
biological_process	GO:0071806	protein transmembrane transport
biological_process	GO:0009451	RNA modification
biological_process	GO:0006420	arginyl-tRNA aminoacylation
biological_process	GO:0006560	proline metabolic process
biological_process	GO:0006353	DNA-templated transcription, termination
biological_process	GO:0006364	rRNA processing
biological_process	GO:0009695	jasmonic acid biosynthetic process
biological_process	GO:0019288	isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process	GO:0009117	nucleotide metabolic process
biological_process	GO:0006733	oxidoreduction coenzyme metabolic process
biological_process	GO:0031408	oxylipin biosynthetic process
biological_process	GO:0006655	phosphatidylglycerol biosynthetic process
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0019216	regulation of lipid metabolic process
biological_process	GO:0009416	response to light stimulus
biological_process	GO:0000304	response to singlet oxygen
biological_process	GO:0019748	secondary metabolic process
biological_process	GO:0009106	lipoate metabolic process
biological_process	GO:0006546	glycine catabolic process
biological_process	GO:0019344	cysteine biosynthetic process
biological_process	GO:0009108	coenzyme biosynthetic process
biological_process	GO:0015995	chlorophyll biosynthetic process
biological_process	GO:0006636	unsaturated fatty acid biosynthetic process
biological_process	GO:0030154	cell differentiation
biological_process	GO:0006766	vitamin metabolic process
biological_process	GO:0016117	carotenoid biosynthetic process
biological_process	GO:0009073	aromatic amino acid family biosynthetic process
biological_process	GO:0009965	leaf morphogenesis
cellular_component	GO:0009707	chloroplast outer membrane
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0009345	glycine-tRNA ligase complex
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0031969	chloroplast membrane
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0015450	P-P-bond-hydrolysis-driven protein transmembrane transporter activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0004822	isoleucine-tRNA ligase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0002161	aminoacyl-tRNA editing activity
molecular_function	GO:0004814	arginine-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004820	glycine-tRNA ligase activity
molecular_function	GO:0005515	protein binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo02935	0.69	Barchart \| Table
Spo00729	0.68	Barchart \| Table
Spo03884	0.68	Barchart \| Table
Spo15422	0.68	Barchart \| Table
Spo09020	0.67	Barchart \| Table
Spo14086	0.67	Barchart \| Table
Spo20456	0.66	Barchart \| Table
Spo04066	0.66	Barchart \| Table
Spo10072	0.65	Barchart \| Table