Spo14080 (gene) | SpinachBase

Overview


Name	Spo14080
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Kinesin-like protein
Location	chr4 : 80960069 .. 80963347 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCACACCCGCCCCATCACCCATGGCGGGTACGATTTTTATACCCATCCCCGCCCCATCACCCGCCAAACCTACTCCCATCCTCGCCTACTTGGGGCAGATGCCGGGCGGGTCTCCCGCGAAACCCGCCCCATTGACATCCCTACTCCCTCTCCTTCCTTCTCTCTTTCTCCAGCCTTCATCCCCTTCCTCTCCTGTTTTTGCTTCCAATGAGCACCTACAGCTCTTTTCCGTCAGGAGCAGTCTCAAAATAATTGAGATATACTGATGGCAGTCTTCTTTCGTGGTTTTGTTGATAGATTGTCATTTCCTTTTTCAGAAGAATTCAATACACAGAAAATTCAAAAGGAAAACAACGGGGTCTAGAAATTGGAAAACCTAAGAAGTAATCTCCGACCCATTTTACAAATCTTACACCCACTCAAATTTTTTTGGGTTTTTCAGCAATTCCCCATCAGATTTTCAGTTTTTTTTTTTTTTTTAAAGGTGAGAGATTCGTAATTTCTTTGTTACGAATCAATGTTTGCACAATTGGTTGGTGGTTTTTTTTAATTAAATTTTTTAACTGTAATTGATTGAATTCATTTTGTTTTCTGGGTTTTGTTGGGGTTTGGTGTGTGATTTGGGGAATTCTGGTTAAAATTAGTTCGATCCGAGATGATGCAGATATACTGGTTTTAGTTTGATTTTATGGGAATATCGAAAGGGGGAATATTCATGGTTTAGGTTTTTGATAAAAAATTTGTCAGAGGTTTAGATAATTAGATCATCATTAATATTTCTTTCACTGCTGTCGGTTGATGTTTGATGAAAGAAATGATTTTTGGGTAAGATCGAACAGTTTGCCATATGATTCTTTTTAGAATAGTATTGTTCTTGTATCTTATCTTACTATCTTAAGGTTGTTGATGTAATTTAAGGTTTCTCTGGTCTGGATGATGTTTTACTTCCTACTTGGTCATGAAAATTGTGTGTTTTGGATTATTCTAATTTGATGATATCTATCATCAATCAATTAATTGGGTGGGCCCTATGGCAGAAGTTTGAATGTTCTTTCCTTTATAATTTGAGTGATCATTTTGTGATTATGCATCTATTGCTTGTAGTGATCGTTCTTGTGATGTGTTTGCTTTTATAGTCTCTTTTGTGCAACAAAATGAATAAGATTGGCCTTGGGTTTTGATAGATTTTGCAGGCGAAATGGTTGATGTTGATAGTAAACATTGAATAGAAGACTCTTTGTGGTATGTTCTCCTTGATATGTTGCATAAGAGGTTGTTAGATTATAGATTCAATGGAAATTAGGTGTATTTCATCCATAGAGCTGCTAGATCTACTAGGGTAATTACGTTTGAATGTGTTTCTAACTTACAAAAGGAACACCCTAAGGAAACAAAGCTATGTTTGTTCATAGTGTATAAATATTGCGAGACCGTTGTTTCGATCTCATTGATAATCTCAACTTACCTATCTGCAGTTATGAAACCTGATCGTGCAAAGCTTAAGATCAAAGTGTTATTGAATAAAAAATGGTGAGTCTAACTACTAATAATTTATCATTGGTGGTGACAGTGATTTCTATTTTTATGCTTAATGGGCTTACAAAATCTCATTTAATATTTCAGCTCTTGGAGCCTTGGAGGAGGAAGAATGATTTTTTTTTTGAAGAATGATTTCAAGGGTATTGTGGCTCCTAACACGTTCAAGGTTGGGCTCGAGGAAGATGGTTTGAGATTGCTGCTGGTTAAACGTAGGTTGTTGTGTTTTTTTAGTACTCGGACTGGTACTGGGAGGGATTTCCTAAGGTAATTGTTTATATTTTTACATTTGATTTTTTGGTAAATTGATTGAATTTTGCTGAGGTTTTGATTTGTTCATTGTATTCATTTATATAATTCTTGAAATTGTGGATGTTACCGGTAGTTGGAAATTAACTTAATTGAAAGTGAGCTCCTGGTGATTTGGTTGACTGGTAGAAAATTGGTGTTCGTGGTTGAGCTGATTTGTCGAGATTTTGTTTCTTGAATGAATTTTGTGTATTTGAAAAGCCGGGTTGTGTAGTAGATACATGCTTGATTAGTTGAAACCTTTGCTGCAGTTTGACGTACTAATTGTCACATAATTCAGCCTGATGTATATTGATCGTCACATAAACCTTTGCCATAGGGCCCACCCAATACATCGCAGATGAGGGCATTAAGGAATCACACGCTCATCCGACTATTACTATTTGAAGACTTCCTCTAAAAAGTGTTAGATCATATTCAGTTTGTAGTATACTGGCATTACCTGGTTATGGGAATTCCCTGGATACGATTCTATCCCCCCCCTTTTAAGTGTATCGGGGAGCTTGCTATGAGCTGAAAATATCAAAACATAGTCTCTGCTTGACAGAATGGCAGTACACAGACCCCCCACAGTAGAGGCATATCAAGGTTGAACGTTTTAGCTCATGTAGGTACAAAATTGCATTGTCGATTCATGTTTTGTGCATAATTCTGTTAAAGTCTCCAGATTATGTTGGTAGGTGCATGTTGTCTTCCTGCTTATGTAAGATGTTAAGTTAGAGGTAGTCCTTACTCTTGTTATATCTGCAAGAGATTGCTTTTTGCTGGAAATTATGCAAGATGACTAGATGAGCTATATGTTTCTCCACTGCCCTATTTACTTTTTCGTAGCAAAATCAAGGAAAAATACTGATGTTTTTCTTATTTCCTGAAAATTTGTCTTTAATTGGAAAATCTGCTATTTGTCATAGTCTGTTGAAGAGTTATAACGTAGGATGTTTAGAAGCCTTCCCACTGAGCCAGTAGTGCATTCTCGACATTCTCGATTGAAGTGTTATCCTAATTAGCAATCGTATTTTGCGATTTAGCCCTTGTGTCATTGCTTTTACACATTTTGATCGTGTTTTTGATAGTTTCAGTTTACTTTCTAAATTTAATCTGACTTAAATTCTTTAGTTTCAACTTTGGCGGCTTGAGTGGTTTTGTGACCCTGCAAAGCATGCTTTGATGTGCACATTACCAGTGTGTCGAACTAAGAATACAAAGTTATGGCTAATTCAATTACAATTCATGTGTTGTATTATAATTGTCAGTTTATTCAATTTCTTCTTCAAGTTGTCAAACCAACATGACTTTATTGATGTTCTGCGACAATTTTTTATTGATCTGGCTGGAAGTGAGGGTGCATCTCAGGCAATGTCAGCTGGGACACAACTAAAGGAAGGTTGCCACATAAACTGGAGTTTGCTGACACTAGGGAATGCTATCAGCTAA

mRNA sequence

CCACACCCGCCCCATCACCCATGGCGGGTACGATTTTTATACCCATCCCCGCCCCATCACCCGCCAAACCTACTCCCATCCTCGCCTACTTGGGGCAGATGCCGGGCGGGTCTCCCGCGAAACCCGCCCCATTGACATCCCTACTCCCTCTCCTTCCTTCTCTCTTTCTCCAGCCTTCATCCCCTTCCTCTCCTGTTTTTGCTTCCAATGAGCACCTACAGCTCTTTTCCGTCAGGAGCAGTCTCAAAATAATTGAGATATACTGATGGCAGTCTTCTTTCGTGGTTTTGTTGATAGATTGTCATTTCCTTTTTCAGAAGAATTCAATACACAGAAAATTCAAAAGGAAAACAACGGGGTCTAGAAATTGGAAAACCTAAGAAGTAATCTCCGACCCATTTTACAAATCTTACACCCACTCAAATTTTTTTGGGTTTTTCAGCAATTCCCCATCAGATTTTCAGTTTTTTTTTTTTTTTTAAAGGTGAGAGATTCGTAATTTCTTTGTTACGAATCAATGTTTGCACAATTGGTTGGTGGTTTTTTTTAATTAAATTTTTTAACTGTAATTGATTGAATTCATTTTGTTTTCTGGGTTTTGTTGGGGTTTGGTGTGTGATTTGGGGAATTCTGGTTAAAATTAGTTCGATCCGAGATGATGCAGATATACTGGTTTTAGTTTGATTTTATGGGAATATCGAAAGGGGGAATATTCATGGTTTAGGTTTTTGATAAAAAATTTGTCAGAGGTTTAGATAATTAGATCATCATTAATATTTCTTTCACTGCTGTCGGTTGATGTTTGATGAAAGAAATGATTTTTGGGTAAGATCGAACAGTTTGCCATATGATTCTTTTTAGAATAGTATTGTTCTTGTATCTTATCTTACTATCTTAAGGTTGTTGATGTAATTTAAGGTTTCTCTGGTCTGGATGATGTTTTACTTCCTACTTGGTCATGAAAATTGTGTGTTTTGGATTATTCTAATTTGATGATATCTATCATCAATCAATTAATTGGGTGGGCCCTATGGCAGAAGTTTGAATGTTCTTTCCTTTATAATTTGAGTGATCATTTTGTGATTATGCATCTATTGCTTGTAGTGATCGTTCTTGTGATGTGTTTGCTTTTATAGTCTCTTTTGTGCAACAAAATGAATAAGATTGGCCTTGGGTTTTGATAGATTTTGCAGGCGAAATGGTTGATGTTGATAGTAAACATTGAATAGAAGACTCTTTGTGTTATGAAACCTGATCGTGCAAAGCTTAAGATCAAAGTGTTATTGAATAAAAAATGCTCTTGGAGCCTTGGAGGAGGAAGAATGATTTTTTTTTTGAAGAATGATTTCAAGGGTATTGTGGCTCCTAACACGTTCAAGGTTGGGCTCGAGGAAGATGGTTTGAGATTGCTGCTGGTTAAACGTAGGTTGTTGTGTTTTTTTAGTACTCGGACTGGTACTGGGAGGGATTTCCTAAGTGAGGGTGCATCTCAGGCAATGTCAGCTGGGACACAACTAAAGGAAGGTTGCCACATAAACTGGAGTTTGCTGACACTAGGGAATGCTATCAGCTAA

Coding sequence (CDS)

ATGAAACCTGATCGTGCAAAGCTTAAGATCAAAGTGTTATTGAATAAAAAATGCTCTTGGAGCCTTGGAGGAGGAAGAATGATTTTTTTTTTGAAGAATGATTTCAAGGGTATTGTGGCTCCTAACACGTTCAAGGTTGGGCTCGAGGAAGATGGTTTGAGATTGCTGCTGGTTAAACGTAGGTTGTTGTGTTTTTTTAGTACTCGGACTGGTACTGGGAGGGATTTCCTAAGTGAGGGTGCATCTCAGGCAATGTCAGCTGGGACACAACTAAAGGAAGGTTGCCACATAAACTGGAGTTTGCTGACACTAGGGAATGCTATCAGCTAA

Protein sequence

MKPDRAKLKIKVLLNKKCSWSLGGGRMIFFLKNDFKGIVAPNTFKVGLEEDGLRLLLVKRRLLCFFSTRTGTGRDFLSEGASQAMSAGTQLKEGCHINWSLLTLGNAIS

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo14080.1	Spo14080.1	mRNA

Homology

[Close]

BLAST of Spo14080.1 vs. NCBI nr
Match: gi|902157691|gb|KNA06154.1| (hypothetical protein SOVF_183740 [Spinacia oleracea])

HSP 1 Score: 223.0 bits (567), Expect = 2.500e-55
Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 1


		  
Query: 1   MKPDRAKLKIKVLLNKKCSWSLGGGRMIFFLKNDFKGIVAPNTFKVGLEEDGLRLLLVKR 60
           MKPDRAKLKIKVLLNKKCSWSLGGGRMIFFLKNDFKGIVAPNTFKVGLEEDGLRLLLVKR
Sbjct: 1   MKPDRAKLKIKVLLNKKCSWSLGGGRMIFFLKNDFKGIVAPNTFKVGLEEDGLRLLLVKR 60

Query: 61  RLLCFFSTRTGTGRDFLSEGASQAMSAGTQLKEGCHINWSLLTLGNAIS 110
           RLLCFFSTRTGTGRDFLSEGASQAMSAGTQLKEGCHINWSLLTLGNAIS
Sbjct: 61  RLLCFFSTRTGTGRDFLSEGASQAMSAGTQLKEGCHINWSLLTLGNAIS 109

[Close]

BLAST of Spo14080.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QFW6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_183740 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.800e-55
Identity = 109/109 (100.00%), Postives = 109/109 (100.00%), Query Frame = 1


		  
Query: 1   MKPDRAKLKIKVLLNKKCSWSLGGGRMIFFLKNDFKGIVAPNTFKVGLEEDGLRLLLVKR 60
           MKPDRAKLKIKVLLNKKCSWSLGGGRMIFFLKNDFKGIVAPNTFKVGLEEDGLRLLLVKR
Sbjct: 1   MKPDRAKLKIKVLLNKKCSWSLGGGRMIFFLKNDFKGIVAPNTFKVGLEEDGLRLLLVKR 60

Query: 61  RLLCFFSTRTGTGRDFLSEGASQAMSAGTQLKEGCHINWSLLTLGNAIS 110
           RLLCFFSTRTGTGRDFLSEGASQAMSAGTQLKEGCHINWSLLTLGNAIS
Sbjct: 61  RLLCFFSTRTGTGRDFLSEGASQAMSAGTQLKEGCHINWSLLTLGNAIS 109

[Close]

BLAST of Spo14080.1 vs. ExPASy Swiss-Prot
Match: KN7F_ARATH (Kinesin-like protein KIN-7F OS=Arabidopsis thaliana GN=KIN7F PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 3.700e-6
Identity = 26/31 (83.87%), Postives = 27/31 (87.10%), Query Frame = 1


		  
Query: 78  SEGASQAMSAGTQLKEGCHINWSLLTLGNAI 109
           SE ASQAMSAGT+LKEGCHIN SLLTLG  I
Sbjct: 244 SERASQAMSAGTRLKEGCHINRSLLTLGTVI 274

[Close]

BLAST of Spo14080.1 vs. TAIR (Arabidopsis)
Match: AT4G38950.1 (ATP binding microtubule motor family protein)

HSP 1 Score: 52.4 bits (124), Expect = 2.100e-7
Identity = 26/31 (83.87%), Postives = 27/31 (87.10%), Query Frame = 1


		  
Query: 78  SEGASQAMSAGTQLKEGCHINWSLLTLGNAI 109
           SE ASQAMSAGT+LKEGCHIN SLLTLG  I
Sbjct: 244 SERASQAMSAGTRLKEGCHINRSLLTLGTVI 274

[Close]

BLAST of Spo14080.1 vs. TAIR (Arabidopsis)
Match: AT5G66310.1 (ATP binding microtubule motor family protein)

HSP 1 Score: 50.8 bits (120), Expect = 6.000e-7
Identity = 30/47 (63.83%), Postives = 35/47 (74.47%), Query Frame = 1


		  
Query: 66  FSTRTGTGRDFL----SEGASQAMSAGTQLKEGCHINWSLLTLGNAI 109
           FST T T  +F+    SE ASQ++SAGT+LKEGCHIN SLLTLG  I
Sbjct: 236 FSTLTATV-NFIDLAGSERASQSLSAGTRLKEGCHINRSLLTLGTVI 281

[Close]

BLAST of Spo14080.1 vs. TAIR (Arabidopsis)
Match: AT5G42490.1 (ATP binding microtubule motor family protein)

HSP 1 Score: 49.7 bits (117), Expect = 1.300e-6
Identity = 24/31 (77.42%), Postives = 26/31 (83.87%), Query Frame = 1


		  
Query: 78  SEGASQAMSAGTQLKEGCHINWSLLTLGNAI 109
           SE ASQ +SAGT+LKEGCHIN SLLTLG  I
Sbjct: 239 SERASQTLSAGTRLKEGCHINRSLLTLGTVI 269

[Close]

BLAST of Spo14080.1 vs. TAIR (Arabidopsis)
Match: AT2G21300.1 (ATP binding microtubule motor family protein)

HSP 1 Score: 49.3 bits (116), Expect = 1.800e-6
Identity = 24/31 (77.42%), Postives = 26/31 (83.87%), Query Frame = 1


		  
Query: 78  SEGASQAMSAGTQLKEGCHINWSLLTLGNAI 109
           SE ASQA+SAG +LKEGCHIN SLLTLG  I
Sbjct: 255 SERASQALSAGARLKEGCHINRSLLTLGTVI 285

[Close]

BLAST of Spo14080.1 vs. TAIR (Arabidopsis)
Match: AT4G24170.1 (ATP binding microtubule motor family protein)

HSP 1 Score: 48.1 bits (113), Expect = 3.900e-6
Identity = 23/31 (74.19%), Postives = 26/31 (83.87%), Query Frame = 1


		  
Query: 78  SEGASQAMSAGTQLKEGCHINWSLLTLGNAI 109
           SE ASQ +SAG++LKEGCHIN SLLTLG  I
Sbjct: 235 SERASQTLSAGSRLKEGCHINRSLLTLGTVI 265

The following BLAST results are available for this feature:

BLAST of Spo14080.1 vs. NCBI nr
BLAST of Spo14080.1 vs. UniProtKB/TrEMBL
BLAST of Spo14080.1 vs. ExPASy Swiss-Prot
BLAST of Spo14080.1 vs. TAIR (Arabidopsis)

BLAST of Spo14080.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 1


Match Name	E-value	Identity	Description
gi\|902157691\|gb\|KNA06154.1\|	2.5e-55	100.	hypothetical protein SOVF_1837...	[more]

BLAST of Spo14080.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 1


Match Name	E-value	Identity	Description
A0A0K9QFW6_SPIOL	1.8e-55	100.	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo14080.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1


Match Name	E-value	Identity	Description
KN7F_ARATH	3.7e-6	83.8	Kinesin-like protein KIN-7F OS...	[more]

BLAST of Spo14080.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G38950.1	2.1e-7	83.8	ATP binding microtubule motor ...	[more]
AT5G66310.1	6.0e-7	63.8	ATP binding microtubule motor ...	[more]
AT5G42490.1	1.3e-6	77.4	ATP binding microtubule motor ...	[more]
AT2G21300.1	1.8e-6	77.4	ATP binding microtubule motor ...	[more]
AT4G24170.1	3.9e-6	74.1	ATP binding microtubule motor ...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001752	Kinesin motor domain	GENE3D	3.40.850.10		coord: 78..109 score: 2.

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0007018	microtubule-based movement
cellular_component	GO:0005874	microtubule
cellular_component	GO:0045298	tubulin complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008017	microtubule binding
molecular_function	GO:0003777	microtubule motor activity
molecular_function	GO:0004185	serine-type carboxypeptidase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo14081	0.73	Barchart \| Table
Spo08648	0.69	Barchart \| Table
Spo01311	0.66	Barchart \| Table
Spo09589	0.66	Barchart \| Table
Spo21823	0.66	Barchart \| Table