Homology
BLAST of Spo12258.1 vs. NCBI nr
Match:
gi|902187174|gb|KNA10860.1| (hypothetical protein SOVF_140450 [Spinacia oleracea])
HSP 1 Score: 1635.5 bits (4234), Expect = 0.000e+0
Identity = 799/799 (100.00%), Postives = 799/799 (100.00%), Query Frame = 1
Query: 1 MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK 60
MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK
Sbjct: 1 MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK 60
Query: 61 SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQQIAQR 120
SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQQIAQR
Sbjct: 61 SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQQIAQR 120
Query: 121 KKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSFSSKV 180
KKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSFSSKV
Sbjct: 121 KKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSFSSKV 180
Query: 181 SKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEKRETI 240
SKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEKRETI
Sbjct: 181 SKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEKRETI 240
Query: 241 ESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQVNHPVREDAKP 300
ESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQVNHPVREDAKP
Sbjct: 241 ESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQVNHPVREDAKP 300
Query: 301 PPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQD 360
PPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQD
Sbjct: 301 PPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQD 360
Query: 361 SGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLNDIYGGSRLDILKRMILFCKA 420
SGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLNDIYGGSRLDILKRMILFCKA
Sbjct: 361 SGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLNDIYGGSRLDILKRMILFCKA 420
Query: 421 AVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAH 480
AVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAH
Sbjct: 421 AVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAH 480
Query: 481 QGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQ 540
QGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQ
Sbjct: 481 QGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQ 540
Query: 541 EGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEA 600
EGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEA
Sbjct: 541 EGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEA 600
Query: 601 LQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLR 660
LQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLR
Sbjct: 601 LQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLR 660
Query: 661 HFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVG 720
HFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVG
Sbjct: 661 HFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVG 720
Query: 721 GLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLS 780
GLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLS
Sbjct: 721 GLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLS 780
Query: 781 WDHAAEIYEEVLVAAKYHW 800
WDHAAEIYEEVLVAAKYHW
Sbjct: 781 WDHAAEIYEEVLVAAKYHW 799
BLAST of Spo12258.1 vs. NCBI nr
Match:
gi|731363098|ref|XP_010693244.1| (PREDICTED: granule-bound starch synthase 2, chloroplastic/amyloplastic [Beta vulgaris subsp. vulgaris])
HSP 1 Score: 1297.3 bits (3356), Expect = 0.000e+0
Identity = 636/801 (79.40%), Postives = 702/801 (87.64%), Query Frame = 1
Query: 1 MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK 60
MASLNSF FTI+ R LHNS+GS +L QRRKPLD GG LKF+ H+ ++ HG SSLCCVK
Sbjct: 1 MASLNSFPFTIEKRCFLHNSHGSQTLGCQRRKPLDGGGLLKFMDHNDNYIHGISSLCCVK 60
Query: 61 SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQQIAQR 120
SV GCRR R+ K ++DS ++ED+KET+DA+QATIEKSNKVLAMQKELLQQIA+R
Sbjct: 61 SVSGCRRRDLRRINLLKAISDDSGEAEDNKETEDAIQATIEKSNKVLAMQKELLQQIAER 120
Query: 121 KKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSFSSKV 180
KKLVSSIKSSI+N + EPF +NDNS PKVD NQT VN EH SG S+RNI S SSKV
Sbjct: 121 KKLVSSIKSSIVNRDGEPFANDNDNSSPKVDLGPNQTHVNTEHNSGADSARNIHSISSKV 180
Query: 181 SKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEKRETI 240
SK Q+PIPVNSR+GR Q K SL T+NL +SMQK+ T PEKPP+KSDG SS+ KRE
Sbjct: 181 SKEQLPIPVNSRDGRREQVKSSLKTINLDTSMQKQVPTGTPEKPPTKSDGSSSDGKREIT 240
Query: 241 ESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQ-EQLEVGEQVNHPVREDAK 300
E LS RT+YG++T + T E +M P FL TY SSIKDE+ +QLE E VN PV EDAK
Sbjct: 241 ERLSHGRTAYGVDTSRTTTETTMLPNFLPRTYKSSSIKDEKVKQLEDDELVNDPVGEDAK 300
Query: 301 PPPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQ 360
PPPLAGANVMNV+LVAAECAPW KTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYA+ Q
Sbjct: 301 PPPLAGANVMNVILVAAECAPWCKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYAETQ 360
Query: 361 DSGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLN-DIYGGSRLDILKRMILFC 420
D+GVRKRYRV+GQD+EV YF T++DGVDFVFI+AP FRHL DIYGGSRLDILKRMILFC
Sbjct: 361 DTGVRKRYRVDGQDVEVCYFQTYIDGVDFVFIDAPFFRHLGKDIYGGSRLDILKRMILFC 420
Query: 421 KAAVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNI 480
KAAVEVPW+VPCGG CYGDGNLVFIANDWHTALLPVYLKAYYRD GLM YTRS+LVIHNI
Sbjct: 421 KAAVEVPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDQGLMKYTRSLLVIHNI 480
Query: 481 AHQGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELK 540
AHQGRGPVDDFSYVDLP HY+DYFK++DPVGGEHFNIFA GL+AADRIVTVSHGYAWELK
Sbjct: 481 AHQGRGPVDDFSYVDLPSHYLDYFKLFDPVGGEHFNIFAGGLRAADRIVTVSHGYAWELK 540
Query: 541 TQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCK 600
TQEGGWGL+ IINE+DWK+RGIVNGIDVTEWNP+LD HL SDGYTNYSL+ +KPGK+QCK
Sbjct: 541 TQEGGWGLHDIINESDWKMRGIVNGIDVTEWNPQLDVHLQSDGYTNYSLKTIKPGKAQCK 600
Query: 601 EALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQM 660
ALQKELGLPVR+DVPL+GFIGRLDDQKGVDIIA+SIPWLMGLDVQLVMLGTGR DLEQ+
Sbjct: 601 AALQKELGLPVREDVPLMGFIGRLDDQKGVDIIADSIPWLMGLDVQLVMLGTGRPDLEQL 660
Query: 661 LRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHA 720
LR FE QY DK+RGWVGFSV+ AHRITAG DILLMPSRFEPCGLNQLYAMNYGTIPVVHA
Sbjct: 661 LRQFESQYHDKIRGWVGFSVKMAHRITAGADILLMPSRFEPCGLNQLYAMNYGTIPVVHA 720
Query: 721 VGGLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQD 780
VGGL+DTVQPFDPYNN+GLGWTFDRAEAHRL+HALGNCLLT+RQYKDSWE LQ RGM+QD
Sbjct: 721 VGGLRDTVQPFDPYNNTGLGWTFDRAEAHRLIHALGNCLLTFRQYKDSWEALQTRGMTQD 780
Query: 781 LSWDHAAEIYEEVLVAAKYHW 800
LSWDHAAEIYEEVLVAAKY W
Sbjct: 781 LSWDHAAEIYEEVLVAAKYQW 801
BLAST of Spo12258.1 vs. NCBI nr
Match:
gi|77176914|gb|ABA64552.1| (starch synthase II [Amaranthus cruentus])
HSP 1 Score: 1157.9 bits (2994), Expect = 0.000e+0
Identity = 592/814 (72.73%), Postives = 665/814 (81.70%), Query Frame = 1
Query: 1 MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK 60
MASLNS F I++R LHNSNGSH L+YQRRKP++ GGFLKF+ H ++GR SL CVK
Sbjct: 1 MASLNSLRFMIETRCSLHNSNGSHHLSYQRRKPVNGGGFLKFMDHD---SNGRLSLYCVK 60
Query: 61 SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKE----TDDAVQATIEKSNKVLAMQKELLQQ 120
SV GCRR S R K +D ++ E SKE +DA+ A IEKSNKV AMQKELL Q
Sbjct: 61 SVSGCRRSNSMRYNRLKAIGDDLNEGEHSKEDKDENEDALDAAIEKSNKVSAMQKELLLQ 120
Query: 121 IAQRKKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSF 180
IA+RKKLVSSI++S+IN ++ P ++ NSP NQ V NE+ T ++R DS
Sbjct: 121 IAERKKLVSSIRNSVINSDDAPADKDY-NSPQNDALGANQILVKNEYDISTGAARQSDSA 180
Query: 181 SSKVSKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEK 240
SSK++ Q+ +P++S E +SGQ K +L LNL SS KE LT+A E S S+G K
Sbjct: 181 SSKLNPDQLQVPLSSLEEKSGQAKSALKMLNLDSSQPKEILTKATETDSSISNGSPIVGK 240
Query: 241 RE--TIESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQ----- 300
RE TI SLS E + G++ K +E +M P FLS+T SSI D++E+ E+GE
Sbjct: 241 REMKTIGSLSSEGNANGVDASKGGDERTMLPNFLSSTNKSSSIMDKEEK-ELGESYFKGL 300
Query: 301 ---VNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVM 360
VN ED KPPPLAG NVMNV+LVAAECAPW KTGGLGDVAG+LPKALARRGHRVM
Sbjct: 301 SELVNDAAAEDDKPPPLAGQNVMNVILVAAECAPWCKTGGLGDVAGALPKALARRGHRVM 360
Query: 361 VVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLN-DIYGG 420
VV PRY +YAD Q +GVRKRY+V GQD EV YFHT++DGVDFVFIEAPM R+L DIYGG
Sbjct: 361 VVIPRYAEYADCQFTGVRKRYKVAGQDNEVLYFHTYIDGVDFVFIEAPMLRNLGKDIYGG 420
Query: 421 SRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGL 480
+RLDILKRMILFCK AVEVPW+VPCGG CYGDGNLVFIANDWHTALLPVYLKAYYRDNGL
Sbjct: 421 NRLDILKRMILFCKVAVEVPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDNGL 480
Query: 481 MMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADR 540
M YTRSVLVIHNIAHQGRGPVDDF+YVDLPPHY+DYFK+YDPVGGEHFNIFAAGLK ADR
Sbjct: 481 MKYTRSVLVIHNIAHQGRGPVDDFTYVDLPPHYLDYFKLYDPVGGEHFNIFAAGLKTADR 540
Query: 541 IVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNY 600
I+TVSHGYAWELKTQEGGWGL+GIIN+NDWKLRGIVNGIDVTEWNP+LD +L SDGY NY
Sbjct: 541 ILTVSHGYAWELKTQEGGWGLHGIINDNDWKLRGIVNGIDVTEWNPELDLYLQSDGYANY 600
Query: 601 SLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQL 660
SLE LKPGK+QCK ALQ ELGLPVR+DVPLIGFIGRLD QKGVDIIAESIPWL G DVQL
Sbjct: 601 SLETLKPGKAQCKAALQNELGLPVREDVPLIGFIGRLDGQKGVDIIAESIPWLSGQDVQL 660
Query: 661 VMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQL 720
VMLGTGR DLEQMLR E +YPDK+RGWVGFSV+TAHRITAGVDILLMPSRFEPCGLNQL
Sbjct: 661 VMLGTGRPDLEQMLRECESRYPDKIRGWVGFSVKTAHRITAGVDILLMPSRFEPCGLNQL 720
Query: 721 YAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKD 780
YAMNYGTIPVVHAVGGL+DTVQPFDPYNN+G GWTFDRAEA+RL+ ALGNCLLTYRQYK+
Sbjct: 721 YAMNYGTIPVVHAVGGLRDTVQPFDPYNNTGYGWTFDRAEANRLIDALGNCLLTYRQYKE 780
Query: 781 SWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
SWEGLQ RGM+QDLSWDHAAEIYEEVLVAAKY W
Sbjct: 781 SWEGLQTRGMTQDLSWDHAAEIYEEVLVAAKYQW 809
BLAST of Spo12258.1 vs. NCBI nr
Match:
gi|645249486|ref|XP_008230773.1| (PREDICTED: granule-bound starch synthase 2, chloroplastic/amyloplastic [Prunus mume])
HSP 1 Score: 973.4 bits (2515), Expect = 2.400e-280
Identity = 502/815 (61.60%), Postives = 594/815 (72.88%), Query Frame = 1
Query: 1 MASLNSFLFTIDSRG----LLHNSNGSH-SLAYQRRKPLDCGGFLKFLGHSGDFAHGRSS 60
MAS+ S F I+++ LLH++N + +P G ++ G + +
Sbjct: 1 MASIGSLPFMIETKTESSVLLHSNNNRRLRFPFFTHRP---GSSVEVSGINAEVVG---- 60
Query: 61 LCCVKSVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQ 120
C + + C +G W++ + S + ED E +DA++ATIEKS KVLAMQ++LLQ
Sbjct: 61 -MCSRQINWCTKGKLWKIEPLRATGKVSVEGEDGGEPEDALRATIEKSKKVLAMQRDLLQ 120
Query: 121 QIAQRKKLVSSIKSSIINHENEP--FMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNI 180
QIA+R+KLVSSI+SS IN E + F + ND+ P N +S T +
Sbjct: 121 QIAERRKLVSSIQSSSINQEEDKASFEQVNDSFP-------------NVESSSTSGDNTV 180
Query: 181 DSFSSKVSKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAP-EKPPSKSDGRS 240
+ ++ +E + +S G ++K+ P K PS+ D
Sbjct: 181 EDQIGSIASSSYGDSTEDKELETVPSAVSRGL----DKIEKDHGEPLPLNKAPSEVDS-- 240
Query: 241 SNEKRETIESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQ-------EQLE 300
T + T KTE + P+F+STT S+ DE+ E
Sbjct: 241 ---------------TKHVNKTSSKTEWSDALPSFISTTAKTSTPIDEELVDLKEPSLEE 300
Query: 301 VGEQVNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRV 360
V + ED KPPPLAGANVMNV+LVAAECAPW KTGGLGDVAG+LPKALARRGHRV
Sbjct: 301 VNNWETDLMSEDVKPPPLAGANVMNVILVAAECAPWSKTGGLGDVAGALPKALARRGHRV 360
Query: 361 MVVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHL-NDIYG 420
MVV P YGDYA+ Q SG+RK Y+V+GQD+EV YFH F+DGVDFVFIE PMFRH+ +DIYG
Sbjct: 361 MVVAPLYGDYAEPQYSGIRKIYKVDGQDLEVAYFHAFIDGVDFVFIECPMFRHMGHDIYG 420
Query: 421 GSRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNG 480
G R DILKRM+LFCKAAVEVPWYVPCGG CYGDGNLVFIANDWHTALLPVYLKAYYRDNG
Sbjct: 421 GKREDILKRMVLFCKAAVEVPWYVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDNG 480
Query: 481 LMMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAAD 540
LM YTRS+LVIHNIAHQGRGPV DFS+VDLP HY+D FK+YDPVGGEHFNIFAAGLK AD
Sbjct: 481 LMTYTRSILVIHNIAHQGRGPVADFSFVDLPGHYLDLFKLYDPVGGEHFNIFAAGLKTAD 540
Query: 541 RIVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTN 600
R+VTVSHGYAWE+KT EGGWGL+GIINENDWK +GIVNGID EWNP+LD +L SDGYTN
Sbjct: 541 RVVTVSHGYAWEVKTVEGGWGLHGIINENDWKFKGIVNGIDTQEWNPQLDVYLTSDGYTN 600
Query: 601 YSLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQ 660
YSL L+ GK QCK ALQKELGLP+R+DVP+IGFIGRLD QKGVD+IAE+IPW+MG DVQ
Sbjct: 601 YSLATLQTGKPQCKAALQKELGLPIREDVPVIGFIGRLDQQKGVDLIAEAIPWMMGQDVQ 660
Query: 661 LVMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQ 720
L+MLG+GR DLEQML+ FE Q+ DKVRGWVGFSV+TAHRITAG DILLMPSRFEPCGLNQ
Sbjct: 661 LIMLGSGRPDLEQMLKEFEHQHRDKVRGWVGFSVQTAHRITAGADILLMPSRFEPCGLNQ 720
Query: 721 LYAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYK 780
LYAMNYGT+PVVHAVGGL+DTVQPF+PY+ SGLGWTFD AEA +L+HAL NCLLTYR+YK
Sbjct: 721 LYAMNYGTVPVVHAVGGLRDTVQPFNPYDESGLGWTFDSAEAGKLIHALWNCLLTYREYK 773
Query: 781 DSWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
SWEG+QRRGM+QDLSWDHAA+ YEEVLVAAKY W
Sbjct: 781 KSWEGIQRRGMTQDLSWDHAAQNYEEVLVAAKYQW 773
BLAST of Spo12258.1 vs. NCBI nr
Match:
gi|731419596|ref|XP_002278470.2| (PREDICTED: granule-bound starch synthase 2, chloroplastic/amyloplastic isoform X2 [Vitis vinifera])
HSP 1 Score: 972.6 bits (2513), Expect = 4.100e-280
Identity = 497/750 (66.27%), Postives = 574/750 (76.53%), Query Frame = 1
Query: 53 RSSLCCVKSVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKE 112
R SLC + V G R V EG S + ED +DA ATIEKS KVLAMQ++
Sbjct: 62 RFSLCSRRQVLGSRG-----VRARGEG---SVEGEDGDRLEDAFDATIEKSKKVLAMQRD 121
Query: 113 LLQQIAQRKKLVSSIKSSIINHE-NEPFMRENDNSPPKVDF-STNQTRVNNEHTSGTVSS 172
LLQQIA+R+KLVSSIKSSIIN E NE + D+S P +D ST + + ++ G +S
Sbjct: 122 LLQQIAERRKLVSSIKSSIINPEDNEVSYKGRDSSFPNMDLTSTGDSGGDEDYNGGILSG 181
Query: 173 RNIDSFSSKVSKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDG 232
+ S + K VP ++S R G G+ G LG + E+ + E P D
Sbjct: 182 NYVHSNADK-----VPAALSSATSR-GFGE---GEKELGKDLSLENPSLDLEAPKQLKDT 241
Query: 233 RSSNEKRETIESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQV 292
+ + S + S +PK+ ++ + E EV +
Sbjct: 242 SPKTVWSDPLPSFLSK--SVETASPKEEKQEDFR---------------ELSSEEVNNEA 301
Query: 293 NHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTP 352
+ ED KPPPLAG NVMN++LVAAECAPW KTGGLGDVAG+LPKALARRGHRVMVV P
Sbjct: 302 AVSMGEDVKPPPLAGTNVMNIILVAAECAPWSKTGGLGDVAGALPKALARRGHRVMVVAP 361
Query: 353 RYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHL-NDIYGGSRLD 412
RYG+YA+ Q++GVRK+Y+V+GQD+EV YF ++DGVDFVFI++ MFRH+ +IYGG+R+D
Sbjct: 362 RYGNYAEAQETGVRKKYKVDGQDMEVTYFQAYIDGVDFVFIDSHMFRHIEKNIYGGNRMD 421
Query: 413 ILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYT 472
ILKRM+LFCKAA+EVPW+VPCGG CYGDGNLVFIANDWHTALLPVYLKAYYRDNGLM YT
Sbjct: 422 ILKRMVLFCKAAIEVPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMQYT 481
Query: 473 RSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTV 532
RS LVIHNIAHQGRGPV+DFSY LP HY+D FK+YDPVGGEHFNIFAAGLK ADR+VTV
Sbjct: 482 RSALVIHNIAHQGRGPVEDFSYTGLPEHYLDLFKLYDPVGGEHFNIFAAGLKTADRVVTV 541
Query: 533 SHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLEN 592
SHGYAWELKT EGGWGL+ IINENDWKLRGIVNGIDV +WNP+LD +L SDGY NYSLE
Sbjct: 542 SHGYAWELKTSEGGWGLHQIINENDWKLRGIVNGIDVKDWNPELDIYLESDGYVNYSLET 601
Query: 593 LKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLG 652
L GK QCK ALQKELGLP+R+DVPLIGFIGRLD QKGVD+IAE++PW++G DVQLVMLG
Sbjct: 602 LHTGKPQCKAALQKELGLPIREDVPLIGFIGRLDHQKGVDLIAEAVPWMVGQDVQLVMLG 661
Query: 653 TGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMN 712
TGR DLEQMLR FE Q+ DK+RGWVGFSV+ AHRITAG DILLMPSRFEPCGLNQLYAMN
Sbjct: 662 TGRNDLEQMLRQFESQHHDKIRGWVGFSVKMAHRITAGADILLMPSRFEPCGLNQLYAMN 721
Query: 713 YGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEG 772
YGTIPVVHAVGGL+DTVQPFDPYN SGLGWTFDRAEA+RL+HALGNCLLTYRQYK SWEG
Sbjct: 722 YGTIPVVHAVGGLRDTVQPFDPYNESGLGWTFDRAEANRLIHALGNCLLTYRQYKQSWEG 777
Query: 773 LQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
LQRRGM QDLSWDHAA+ YEEVLVAAKY W
Sbjct: 782 LQRRGMMQDLSWDHAAQNYEEVLVAAKYQW 777
BLAST of Spo12258.1 vs. UniProtKB/TrEMBL
Match:
A0A0K9QUC8_SPIOL (Starch synthase, chloroplastic/amyloplastic OS=Spinacia oleracea GN=SOVF_140450 PE=3 SV=1)
HSP 1 Score: 1635.5 bits (4234), Expect = 0.000e+0
Identity = 799/799 (100.00%), Postives = 799/799 (100.00%), Query Frame = 1
Query: 1 MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK 60
MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK
Sbjct: 1 MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK 60
Query: 61 SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQQIAQR 120
SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQQIAQR
Sbjct: 61 SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQQIAQR 120
Query: 121 KKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSFSSKV 180
KKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSFSSKV
Sbjct: 121 KKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSFSSKV 180
Query: 181 SKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEKRETI 240
SKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEKRETI
Sbjct: 181 SKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEKRETI 240
Query: 241 ESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQVNHPVREDAKP 300
ESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQVNHPVREDAKP
Sbjct: 241 ESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQVNHPVREDAKP 300
Query: 301 PPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQD 360
PPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQD
Sbjct: 301 PPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQD 360
Query: 361 SGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLNDIYGGSRLDILKRMILFCKA 420
SGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLNDIYGGSRLDILKRMILFCKA
Sbjct: 361 SGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLNDIYGGSRLDILKRMILFCKA 420
Query: 421 AVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAH 480
AVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAH
Sbjct: 421 AVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAH 480
Query: 481 QGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQ 540
QGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQ
Sbjct: 481 QGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQ 540
Query: 541 EGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEA 600
EGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEA
Sbjct: 541 EGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEA 600
Query: 601 LQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLR 660
LQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLR
Sbjct: 601 LQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLR 660
Query: 661 HFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVG 720
HFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVG
Sbjct: 661 HFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVG 720
Query: 721 GLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLS 780
GLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLS
Sbjct: 721 GLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLS 780
Query: 781 WDHAAEIYEEVLVAAKYHW 800
WDHAAEIYEEVLVAAKYHW
Sbjct: 781 WDHAAEIYEEVLVAAKYHW 799
BLAST of Spo12258.1 vs. UniProtKB/TrEMBL
Match:
A0A0J8BE99_BETVU (Starch synthase, chloroplastic/amyloplastic OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g046740 PE=3 SV=1)
HSP 1 Score: 1297.3 bits (3356), Expect = 0.000e+0
Identity = 636/801 (79.40%), Postives = 702/801 (87.64%), Query Frame = 1
Query: 1 MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK 60
MASLNSF FTI+ R LHNS+GS +L QRRKPLD GG LKF+ H+ ++ HG SSLCCVK
Sbjct: 1 MASLNSFPFTIEKRCFLHNSHGSQTLGCQRRKPLDGGGLLKFMDHNDNYIHGISSLCCVK 60
Query: 61 SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQQIAQR 120
SV GCRR R+ K ++DS ++ED+KET+DA+QATIEKSNKVLAMQKELLQQIA+R
Sbjct: 61 SVSGCRRRDLRRINLLKAISDDSGEAEDNKETEDAIQATIEKSNKVLAMQKELLQQIAER 120
Query: 121 KKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSFSSKV 180
KKLVSSIKSSI+N + EPF +NDNS PKVD NQT VN EH SG S+RNI S SSKV
Sbjct: 121 KKLVSSIKSSIVNRDGEPFANDNDNSSPKVDLGPNQTHVNTEHNSGADSARNIHSISSKV 180
Query: 181 SKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEKRETI 240
SK Q+PIPVNSR+GR Q K SL T+NL +SMQK+ T PEKPP+KSDG SS+ KRE
Sbjct: 181 SKEQLPIPVNSRDGRREQVKSSLKTINLDTSMQKQVPTGTPEKPPTKSDGSSSDGKREIT 240
Query: 241 ESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQ-EQLEVGEQVNHPVREDAK 300
E LS RT+YG++T + T E +M P FL TY SSIKDE+ +QLE E VN PV EDAK
Sbjct: 241 ERLSHGRTAYGVDTSRTTTETTMLPNFLPRTYKSSSIKDEKVKQLEDDELVNDPVGEDAK 300
Query: 301 PPPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQ 360
PPPLAGANVMNV+LVAAECAPW KTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYA+ Q
Sbjct: 301 PPPLAGANVMNVILVAAECAPWCKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYAETQ 360
Query: 361 DSGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLN-DIYGGSRLDILKRMILFC 420
D+GVRKRYRV+GQD+EV YF T++DGVDFVFI+AP FRHL DIYGGSRLDILKRMILFC
Sbjct: 361 DTGVRKRYRVDGQDVEVCYFQTYIDGVDFVFIDAPFFRHLGKDIYGGSRLDILKRMILFC 420
Query: 421 KAAVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNI 480
KAAVEVPW+VPCGG CYGDGNLVFIANDWHTALLPVYLKAYYRD GLM YTRS+LVIHNI
Sbjct: 421 KAAVEVPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDQGLMKYTRSLLVIHNI 480
Query: 481 AHQGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELK 540
AHQGRGPVDDFSYVDLP HY+DYFK++DPVGGEHFNIFA GL+AADRIVTVSHGYAWELK
Sbjct: 481 AHQGRGPVDDFSYVDLPSHYLDYFKLFDPVGGEHFNIFAGGLRAADRIVTVSHGYAWELK 540
Query: 541 TQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCK 600
TQEGGWGL+ IINE+DWK+RGIVNGIDVTEWNP+LD HL SDGYTNYSL+ +KPGK+QCK
Sbjct: 541 TQEGGWGLHDIINESDWKMRGIVNGIDVTEWNPQLDVHLQSDGYTNYSLKTIKPGKAQCK 600
Query: 601 EALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQM 660
ALQKELGLPVR+DVPL+GFIGRLDDQKGVDIIA+SIPWLMGLDVQLVMLGTGR DLEQ+
Sbjct: 601 AALQKELGLPVREDVPLMGFIGRLDDQKGVDIIADSIPWLMGLDVQLVMLGTGRPDLEQL 660
Query: 661 LRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHA 720
LR FE QY DK+RGWVGFSV+ AHRITAG DILLMPSRFEPCGLNQLYAMNYGTIPVVHA
Sbjct: 661 LRQFESQYHDKIRGWVGFSVKMAHRITAGADILLMPSRFEPCGLNQLYAMNYGTIPVVHA 720
Query: 721 VGGLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQD 780
VGGL+DTVQPFDPYNN+GLGWTFDRAEAHRL+HALGNCLLT+RQYKDSWE LQ RGM+QD
Sbjct: 721 VGGLRDTVQPFDPYNNTGLGWTFDRAEAHRLIHALGNCLLTFRQYKDSWEALQTRGMTQD 780
Query: 781 LSWDHAAEIYEEVLVAAKYHW 800
LSWDHAAEIYEEVLVAAKY W
Sbjct: 781 LSWDHAAEIYEEVLVAAKYQW 801
BLAST of Spo12258.1 vs. UniProtKB/TrEMBL
Match:
Q0H7E2_AMACR (Starch synthase, chloroplastic/amyloplastic OS=Amaranthus cruentus GN=SsII PE=2 SV=1)
HSP 1 Score: 1157.9 bits (2994), Expect = 0.000e+0
Identity = 592/814 (72.73%), Postives = 665/814 (81.70%), Query Frame = 1
Query: 1 MASLNSFLFTIDSRGLLHNSNGSHSLAYQRRKPLDCGGFLKFLGHSGDFAHGRSSLCCVK 60
MASLNS F I++R LHNSNGSH L+YQRRKP++ GGFLKF+ H ++GR SL CVK
Sbjct: 1 MASLNSLRFMIETRCSLHNSNGSHHLSYQRRKPVNGGGFLKFMDHD---SNGRLSLYCVK 60
Query: 61 SVGGCRRGYSWRVGCSKEGTNDSSDSEDSKE----TDDAVQATIEKSNKVLAMQKELLQQ 120
SV GCRR S R K +D ++ E SKE +DA+ A IEKSNKV AMQKELL Q
Sbjct: 61 SVSGCRRSNSMRYNRLKAIGDDLNEGEHSKEDKDENEDALDAAIEKSNKVSAMQKELLLQ 120
Query: 121 IAQRKKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNIDSF 180
IA+RKKLVSSI++S+IN ++ P ++ NSP NQ V NE+ T ++R DS
Sbjct: 121 IAERKKLVSSIRNSVINSDDAPADKDY-NSPQNDALGANQILVKNEYDISTGAARQSDSA 180
Query: 181 SSKVSKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSSNEK 240
SSK++ Q+ +P++S E +SGQ K +L LNL SS KE LT+A E S S+G K
Sbjct: 181 SSKLNPDQLQVPLSSLEEKSGQAKSALKMLNLDSSQPKEILTKATETDSSISNGSPIVGK 240
Query: 241 RE--TIESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGEQ----- 300
RE TI SLS E + G++ K +E +M P FLS+T SSI D++E+ E+GE
Sbjct: 241 REMKTIGSLSSEGNANGVDASKGGDERTMLPNFLSSTNKSSSIMDKEEK-ELGESYFKGL 300
Query: 301 ---VNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVM 360
VN ED KPPPLAG NVMNV+LVAAECAPW KTGGLGDVAG+LPKALARRGHRVM
Sbjct: 301 SELVNDAAAEDDKPPPLAGQNVMNVILVAAECAPWCKTGGLGDVAGALPKALARRGHRVM 360
Query: 361 VVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHLN-DIYGG 420
VV PRY +YAD Q +GVRKRY+V GQD EV YFHT++DGVDFVFIEAPM R+L DIYGG
Sbjct: 361 VVIPRYAEYADCQFTGVRKRYKVAGQDNEVLYFHTYIDGVDFVFIEAPMLRNLGKDIYGG 420
Query: 421 SRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGL 480
+RLDILKRMILFCK AVEVPW+VPCGG CYGDGNLVFIANDWHTALLPVYLKAYYRDNGL
Sbjct: 421 NRLDILKRMILFCKVAVEVPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDNGL 480
Query: 481 MMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADR 540
M YTRSVLVIHNIAHQGRGPVDDF+YVDLPPHY+DYFK+YDPVGGEHFNIFAAGLK ADR
Sbjct: 481 MKYTRSVLVIHNIAHQGRGPVDDFTYVDLPPHYLDYFKLYDPVGGEHFNIFAAGLKTADR 540
Query: 541 IVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNY 600
I+TVSHGYAWELKTQEGGWGL+GIIN+NDWKLRGIVNGIDVTEWNP+LD +L SDGY NY
Sbjct: 541 ILTVSHGYAWELKTQEGGWGLHGIINDNDWKLRGIVNGIDVTEWNPELDLYLQSDGYANY 600
Query: 601 SLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQL 660
SLE LKPGK+QCK ALQ ELGLPVR+DVPLIGFIGRLD QKGVDIIAESIPWL G DVQL
Sbjct: 601 SLETLKPGKAQCKAALQNELGLPVREDVPLIGFIGRLDGQKGVDIIAESIPWLSGQDVQL 660
Query: 661 VMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQL 720
VMLGTGR DLEQMLR E +YPDK+RGWVGFSV+TAHRITAGVDILLMPSRFEPCGLNQL
Sbjct: 661 VMLGTGRPDLEQMLRECESRYPDKIRGWVGFSVKTAHRITAGVDILLMPSRFEPCGLNQL 720
Query: 721 YAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKD 780
YAMNYGTIPVVHAVGGL+DTVQPFDPYNN+G GWTFDRAEA+RL+ ALGNCLLTYRQYK+
Sbjct: 721 YAMNYGTIPVVHAVGGLRDTVQPFDPYNNTGYGWTFDRAEANRLIDALGNCLLTYRQYKE 780
Query: 781 SWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
SWEGLQ RGM+QDLSWDHAAEIYEEVLVAAKY W
Sbjct: 781 SWEGLQTRGMTQDLSWDHAAEIYEEVLVAAKYQW 809
BLAST of Spo12258.1 vs. UniProtKB/TrEMBL
Match:
M5WQU7_PRUPE (Starch synthase, chloroplastic/amyloplastic OS=Prunus persica GN=PRUPE_ppa001734mg PE=3 SV=1)
HSP 1 Score: 968.8 bits (2503), Expect = 4.100e-279
Identity = 504/814 (61.92%), Postives = 596/814 (73.22%), Query Frame = 1
Query: 1 MASLNSFLFTIDSRG----LLHNSNGSH-SLAYQRRKPLDCGGFLKFLGHSGDFAHGRSS 60
MAS+ S F I+++ LLH++N + +P G ++ G + +
Sbjct: 1 MASIGSLPFMIETKTESSVLLHSNNNRRLRFPFFTHRP---GRSIEVTGINAEVVG---- 60
Query: 61 LCCVKSVGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQATIEKSNKVLAMQKELLQ 120
C + + C +G W++ + S + ED E +DA++ATIEKS KVLAMQ++LLQ
Sbjct: 61 -MCSRQINWCTKGKLWKIEPLRATGKVSVEGEDGGEPEDALRATIEKSKKVLAMQRDLLQ 120
Query: 121 QIAQRKKLVSSIKSSIINHENEP--FMRENDNSPPKVDFSTNQTRVNNEHTSGTVSSRNI 180
QIA+R+KLVSSI+SS IN E + F + ND+ P V + TSG
Sbjct: 121 QIAERRKLVSSIQSSSINQEEDKASFEQGNDSFP----------NVESSSTSG------- 180
Query: 181 DSFSSKVSKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTRAPEKPPSKSDGRSS 240
+ Q+ +S G S + K L T+ S + + +P + S
Sbjct: 181 ----DNTVEDQIGSIASSSYGDSTEDK-ELETVPSAVSRGLNKIEKDHGEPLPLNKAPSE 240
Query: 241 NEKRETIESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQ-------EQLEV 300
E + + +S KTE + P+F+STT S+ DE+ EV
Sbjct: 241 VESTKHVNKIS-----------SKTEWSDALPSFISTTAETSTPIDEELVDLKEPSLEEV 300
Query: 301 GEQVNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVM 360
+ ED KPPPLAGANVMNV+LVAAECAPW KTGGLGDVAG+LPKALARRGHRVM
Sbjct: 301 NNWETDLMSEDVKPPPLAGANVMNVILVAAECAPWSKTGGLGDVAGALPKALARRGHRVM 360
Query: 361 VVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHL-NDIYGG 420
VV P YGDYA+ Q SG+RK Y+V+GQD+EV YFH F+DGVDFVFIE PMFRH+ ++IYGG
Sbjct: 361 VVAPLYGDYAEPQYSGIRKIYKVDGQDLEVAYFHAFIDGVDFVFIECPMFRHMGHNIYGG 420
Query: 421 SRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGL 480
R DILKRM+LFCKAAVEVPW+VPCGG CYGDGNLVFIANDWHTALLPVYLKAYYRDNGL
Sbjct: 421 KREDILKRMVLFCKAAVEVPWHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDNGL 480
Query: 481 MMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADR 540
M YTRS+LVIHNIAHQGRGPV DFS+VDLP HY+D FK+YDPVGGEHFNIFAAGLK ADR
Sbjct: 481 MTYTRSILVIHNIAHQGRGPVADFSFVDLPGHYLDLFKLYDPVGGEHFNIFAAGLKTADR 540
Query: 541 IVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNY 600
+VTVSHGYAWE+KT EGGWGL+GIINENDWK +GIVNGID EWNP+LD +L SDGYTNY
Sbjct: 541 VVTVSHGYAWEVKTVEGGWGLHGIINENDWKFKGIVNGIDTQEWNPQLDVYLTSDGYTNY 600
Query: 601 SLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQL 660
SL L+ GK QCK ALQKELGLP+R+DVP+IGFIGRLD QKGVD+IAE+IPW+MG DVQL
Sbjct: 601 SLVTLQTGKPQCKAALQKELGLPIREDVPVIGFIGRLDQQKGVDLIAEAIPWMMGQDVQL 660
Query: 661 VMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQL 720
+MLG+GR DLEQML+ FE Q+ DKVRGWVGFSV+TAHRITAG DILLMPSRFEPCGLNQL
Sbjct: 661 IMLGSGRPDLEQMLKEFEHQHRDKVRGWVGFSVQTAHRITAGADILLMPSRFEPCGLNQL 720
Query: 721 YAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKD 780
YAMNYGTIPVVHAVGGL+DTVQPF+PY+ SGLGWTFD AEA +L+HALGNCLLTYR+YK
Sbjct: 721 YAMNYGTIPVVHAVGGLRDTVQPFNPYDESGLGWTFDSAEAGKLIHALGNCLLTYREYKK 773
Query: 781 SWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
SWEG+QRRGM QDLSWDHAA+ YEEVLVAAKY W
Sbjct: 781 SWEGIQRRGMKQDLSWDHAAQNYEEVLVAAKYQW 773
BLAST of Spo12258.1 vs. UniProtKB/TrEMBL
Match:
A0A087H628_ARAAL (Starch synthase, chloroplastic/amyloplastic OS=Arabis alpina GN=AALP_AA3G001700 PE=3 SV=1)
HSP 1 Score: 966.1 bits (2496), Expect = 2.700e-278
Identity = 495/773 (64.04%), Postives = 586/773 (75.81%), Query Frame = 1
Query: 48 DFAHGRSSLCCVKSVGGCRRGYSWRVGCSKEGT-NDSSDSEDSKETDDAVQATIEKSNKV 107
DFA G S + V G+ W+ E T +DSS++ + E +DA+QATIEKS KV
Sbjct: 39 DFASGSLSFRGLSFV----LGHRWKCVKRVEATGSDSSEAVNDDEQEDALQATIEKSKKV 98
Query: 108 LAMQKELLQQIAQRKKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTRVNNEHTSG 167
L MQ+ LLQQIA+R+KLVSSI+ S N ++ + + V+ +T+ TR G
Sbjct: 99 LEMQRNLLQQIAERRKLVSSIEESTPNLDDGKVSAKEEYDESSVNANTDVTRKEKSDGEG 158
Query: 168 TVSSRNIDSFSSKVSKGQVPIPVNSREG---RSGQGKLSLGTLNLGSSMQKEDLTRAPEK 227
S S ++K ++ G +S Q S+ + + +S QK+ A K
Sbjct: 159 NGSISPSSYGKSSLNKEPEAKSLSPGTGSLKKSNQSSASVKSSSPVTSPQKQSDVVANGK 218
Query: 228 PPSKSDGRSSNEKRETIESLSR-ERTSYGLNTPKKTEENSMQ-------PTFLSTTYIPS 287
P S S + + +++ E+ S + +P+K + P++L++T S
Sbjct: 219 PWSSVIASSQDPPYKPSSTVTTSEKASDPITSPEKPSNSRAGAFWSDPLPSYLTSTSETS 278
Query: 288 SIKDEQEQL--------EVGEQVNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGGL 347
S+K E+ EV + N P +++ PPPLAGANVMNV+LVAAECAP+ KTGGL
Sbjct: 279 SVKTEESMETKVEKAPEEVSSETNEPGKDEEMPPPLAGANVMNVILVAAECAPFSKTGGL 338
Query: 348 GDVAGSLPKALARRGHRVMVVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVD 407
GDVAG+LPKALARRGHRVMVV PRY +YA+ +D GVRKRY+V GQD+EV YFH ++DGVD
Sbjct: 339 GDVAGALPKALARRGHRVMVVVPRYAEYAEAKDVGVRKRYKVAGQDMEVMYFHAYIDGVD 398
Query: 408 FVFIEAPMFRHL-NDIYGGSRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIAND 467
FVFI++P+FRHL N+IY G+RLDIL+RM+LFCKAAVEVPWYVPCGG CYGDGNL FIAND
Sbjct: 399 FVFIDSPVFRHLSNNIYAGNRLDILRRMVLFCKAAVEVPWYVPCGGVCYGDGNLAFIAND 458
Query: 468 WHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYD 527
WHTALLPVYLKAYYRD+GLM YTRSVLVIHNIAHQGRGPVDDFSYVDLP HY+D FK+YD
Sbjct: 459 WHTALLPVYLKAYYRDHGLMKYTRSVLVIHNIAHQGRGPVDDFSYVDLPGHYLDSFKLYD 518
Query: 528 PVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDV 587
PVGGEHFNIFAAGLKAADR++TVSHGY+WE+KT EGGWGL+ IINENDWK RGIVNGID
Sbjct: 519 PVGGEHFNIFAAGLKAADRVLTVSHGYSWEVKTLEGGWGLHNIINENDWKFRGIVNGIDT 578
Query: 588 TEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQK 647
EWNPK D HLHSD YTNYSLENL GK QCK ALQKELGLPVR DVPLIGFIGRLD QK
Sbjct: 579 KEWNPKFDIHLHSDDYTNYSLENLHIGKPQCKAALQKELGLPVRPDVPLIGFIGRLDHQK 638
Query: 648 GVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITA 707
GVD+IAE++PW+M DVQLVMLGTGR DLE++LR E QY DK RGWVGFSV+TAHRITA
Sbjct: 639 GVDLIAEAVPWMMSQDVQLVMLGTGRPDLEEVLRQMEHQYRDKARGWVGFSVKTAHRITA 698
Query: 708 GVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEA 767
G DILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGL+DTVQ FDPY+ +GLGWTFD AEA
Sbjct: 699 GADILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLRDTVQQFDPYSETGLGWTFDSAEA 758
Query: 768 HRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
+L+HALGNCLLTYR+YK+SWEGLQRRGM+QDLSWD+AAE YEEVLVAAKYHW
Sbjct: 759 GKLIHALGNCLLTYREYKESWEGLQRRGMTQDLSWDNAAEKYEEVLVAAKYHW 807
BLAST of Spo12258.1 vs. ExPASy Swiss-Prot
Match:
SSY2_SOLTU (Granule-bound starch synthase 2, chloroplastic/amyloplastic OS=Solanum tuberosum GN=SS2 PE=1 SV=3)
HSP 1 Score: 947.6 bits (2448), Expect = 8.900e-275
Identity = 484/774 (62.53%), Postives = 579/774 (74.81%), Query Frame = 1
Query: 45 HSGDFAHGRSSLCCVKS-----VGGCRRGYSWRVGCSKEGTNDSSDSEDSKETDDAVQAT 104
HSG+ H L ++ + G R WR+ K +S ++ + E++DA+Q T
Sbjct: 8 HSGNQFHPNLPLLALRPKKLSLIHGSSREQMWRIKRVKATGENSGEAASADESNDALQVT 67
Query: 105 IEKSNKVLAMQKELLQQIAQRKKLVSSIKSSIINHENEPFMRE---NDNSPPKVDFSTNQ 164
IEKS KVLAMQ++LLQQIA+R+K+VSSIKSS+ N + +D P VD N
Sbjct: 68 IEKSKKVLAMQQDLLQQIAERRKVVSSIKSSLANAKGTYDGGSGSLSDVDIPDVDKDYNV 127
Query: 165 TRVNNEHTSGTVSSRNIDSFSSK---VSKGQVPIPV-NSREGRSGQGKLSLGTLNLGSSM 224
T + T T +N S+ SK ++ + + R + ++ + +
Sbjct: 128 TVPSTAATPITDVDKNTPPAISQDFVESKREIKRDLADERAPPLSRSSITASSQISSTVS 187
Query: 225 QKEDLTRAPEKPPSKSDGRSS-NEKRETIESLSRERTSYGLNTPKKTEENSMQPTFLSTT 284
K L PE P S + N ++ ++ ++ SY P +E+S
Sbjct: 188 SKRTLNVPPETPKSSQETLLDVNSRKSLVDVPGKKIQSY---MPSLRKESSA-------- 247
Query: 285 YIPSSIKDEQEQLE-----VGEQVNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGG 344
S ++ E LE E+ PV D KPPPLAG NVMN++LVA+ECAPW KTGG
Sbjct: 248 ---SHVEQRNENLEGSSAEANEETEDPVNIDEKPPPLAGTNVMNIILVASECAPWSKTGG 307
Query: 345 LGDVAGSLPKALARRGHRVMVVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGV 404
LGDVAG+LPKALARRGHRVMVV PRY +Y + QDSGVRK Y+V+GQD+EV YF F+DGV
Sbjct: 308 LGDVAGALPKALARRGHRVMVVAPRYDNYPEPQDSGVRKIYKVDGQDVEVTYFQAFIDGV 367
Query: 405 DFVFIEAPMFRHL-NDIYGGSRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIAN 464
DFVFI++ MFRH+ N+IYGG+R+DILKRM+LFCKAA+EVPW+VPCGG CYGDGNLVFIAN
Sbjct: 368 DFVFIDSHMFRHIGNNIYGGNRVDILKRMVLFCKAAIEVPWHVPCGGVCYGDGNLVFIAN 427
Query: 465 DWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMY 524
DWHTALLPVYLKAYYRDNG+M YTRSVLVIHNIAHQGRGP++DFSYVDLPPHY+D FK+Y
Sbjct: 428 DWHTALLPVYLKAYYRDNGIMNYTRSVLVIHNIAHQGRGPLEDFSYVDLPPHYMDPFKLY 487
Query: 525 DPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGID 584
DPVGGEHFNIFAAGLK ADR+VTVSHGY+WELKT +GGWGL+ IINENDWKL+GIVNGID
Sbjct: 488 DPVGGEHFNIFAAGLKTADRVVTVSHGYSWELKTSQGGWGLHQIINENDWKLQGIVNGID 547
Query: 585 VTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQ 644
EWNP+LD HL SDGY NYSL+ L+ GK QCK ALQKELGLPVRDDVPLIGFIGRLD Q
Sbjct: 548 TKEWNPELDVHLQSDGYMNYSLDTLQTGKPQCKAALQKELGLPVRDDVPLIGFIGRLDPQ 607
Query: 645 KGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRIT 704
KGVD+IAE++PW+MG DVQLVMLGTGR+DLEQMLR FE Q+ DK+RGWVGFSV+T+HRIT
Sbjct: 608 KGVDLIAEAVPWMMGQDVQLVMLGTGRRDLEQMLRQFECQHNDKIRGWVGFSVKTSHRIT 667
Query: 705 AGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAE 764
AG DILLMPSRFEPCGLNQLYAM YGTIPVVHAVGGL+DTVQPFDP+N SGLGWTF RAE
Sbjct: 668 AGADILLMPSRFEPCGLNQLYAMKYGTIPVVHAVGGLRDTVQPFDPFNESGLGWTFSRAE 727
Query: 765 AHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
A +L+HALGNCLLTYR+YK SWEG+Q R M+QDLSWD+AA+ YEEVL+AAKY W
Sbjct: 728 ASQLIHALGNCLLTYREYKKSWEGIQTRCMTQDLSWDNAAQNYEEVLIAAKYQW 767
BLAST of Spo12258.1 vs. ExPASy Swiss-Prot
Match:
SSY2_ARATH (Starch synthase 2, chloroplastic/amyloplastic OS=Arabidopsis thaliana GN=SS2 PE=1 SV=1)
HSP 1 Score: 944.1 bits (2439), Expect = 9.800e-274
Identity = 491/773 (63.52%), Postives = 585/773 (75.68%), Query Frame = 1
Query: 45 HSGDFAHGRSSLCCVKSVGGCRRGYSWRVG--CSKEGTNDSSDSEDSKETDDAVQATIEK 104
+S H R S + S R S+ +G C ++S S+D E +DA+QATI+K
Sbjct: 22 NSSTLRHSRVSYHDLPSGSLSFRSRSFVLGHRCKCVSRVEASGSDDD-EPEDALQATIDK 81
Query: 105 SNKVLAMQKELLQQIAQRKKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTR--VN 164
S KVLAMQ+ LL QIA+R+KLVSSIK S + ++ + + S V+ +T+ T+ +
Sbjct: 82 SKKVLAMQRNLLHQIAERRKLVSSIKESTPDLDDAKASSKQE-SASSVNANTDATKKEIM 141
Query: 165 NEHTSGTVSSRNIDSFS-SKVSKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTR 224
+ +G+VS S SK + + P Q S+ + + +S QK
Sbjct: 142 DGDANGSVSPSTYGKSSLSKEPEAKTFSPSTESLKNRKQSSASVISSSPVTSPQKPSDVA 201
Query: 225 APEKPPSKSDGRSSNEKRETIESL-SRERTSYGLNTPKKTEENSMQ-------PTFLSTT 284
KP S S + + + S E+TS + +P K ++ P++L+
Sbjct: 202 TNGKPWSSVVASSVDPPYKPSSVMTSPEKTSDPVTSPGKPSKSRAGAFWSDPLPSYLTKA 261
Query: 285 YIPSSIKDEQEQLEVGE----QVNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGGL 344
S++K E+ + + + N P +++ KPPPLAGANVMNV+LVAAECAP+ KTGGL
Sbjct: 262 PQTSTMKTEKYVEKTPDVASSETNEPGKDEEKPPPLAGANVMNVILVAAECAPFSKTGGL 321
Query: 345 GDVAGSLPKALARRGHRVMVVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVD 404
GDVAG+LPK+LARRGHRVMVV PRY +YA+ +D GVRKRY+V GQD+EV YFH F+DGVD
Sbjct: 322 GDVAGALPKSLARRGHRVMVVVPRYAEYAEAKDLGVRKRYKVAGQDMEVMYFHAFIDGVD 381
Query: 405 FVFIEAPMFRHL-NDIYGGSRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIAND 464
FVFI++P FRHL N+IYGG+RLDILKRM+LFCKAAVEVPWYVPCGG CYGDGNL FIAND
Sbjct: 382 FVFIDSPEFRHLSNNIYGGNRLDILKRMVLFCKAAVEVPWYVPCGGVCYGDGNLAFIAND 441
Query: 465 WHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYD 524
WHTALLPVYLKAYYRD+G+M YTRSVLVIHNIAHQGRGPVDDFSYVDLP HY+D FK+YD
Sbjct: 442 WHTALLPVYLKAYYRDHGIMKYTRSVLVIHNIAHQGRGPVDDFSYVDLPSHYLDSFKLYD 501
Query: 525 PVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDV 584
PVGGEHFNIFAAGLKAADR++TVSHGY+WE+KT EGGWGL+ IINENDWK RGIVNGID
Sbjct: 502 PVGGEHFNIFAAGLKAADRVLTVSHGYSWEVKTLEGGWGLHNIINENDWKFRGIVNGIDT 561
Query: 585 TEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQK 644
EWNP+ D +LHSD YTNYSLENL GK QCK ALQKELGLPVR DVPLIGFIGRLD QK
Sbjct: 562 QEWNPEFDTYLHSDDYTNYSLENLHIGKPQCKAALQKELGLPVRPDVPLIGFIGRLDHQK 621
Query: 645 GVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITA 704
GVD+IAE++PW+M DVQLVMLGTGR DLE++LR E QY DK RGWVGFSV+TAHRITA
Sbjct: 622 GVDLIAEAVPWMMSQDVQLVMLGTGRPDLEEVLRQMEHQYRDKARGWVGFSVKTAHRITA 681
Query: 705 GVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEA 764
G DILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGL+DTVQ FDPY+ +GLGWTFD AEA
Sbjct: 682 GADILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLRDTVQQFDPYSETGLGWTFDSAEA 741
Query: 765 HRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
+L+HALGNCLLTYR+YK+SWEGLQRRGM+QDLSWD+AAE YEEVLVAAKYHW
Sbjct: 742 GKLIHALGNCLLTYREYKESWEGLQRRGMTQDLSWDNAAEKYEEVLVAAKYHW 792
BLAST of Spo12258.1 vs. ExPASy Swiss-Prot
Match:
SSG2_PEA (Granule-bound starch synthase 2, chloroplastic/amyloplastic OS=Pisum sativum PE=1 SV=1)
HSP 1 Score: 868.6 bits (2243), Expect = 5.200e-251
Identity = 403/543 (74.22%), Postives = 465/543 (85.64%), Query Frame = 1
Query: 270 TTYIPSSIKDEQEQLEVGEQVNHPVREDA------------KPPPLAGANVMNVVLVAAE 329
T I SSI+ + E E N P ++ KPPPLAG NVMN++LV+AE
Sbjct: 210 TKDISSSIRTSSLKFENFEGANEPSSKEVANEAENFESGGEKPPPLAGTNVMNIILVSAE 269
Query: 330 CAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQDSGVRKRYRVEGQDIEVG 389
CAPW KTGGLGDVAGSLPKALARRGHRVM+V P YG+YA+ D GVRKRY+V GQD+EV
Sbjct: 270 CAPWSKTGGLGDVAGSLPKALARRGHRVMIVAPHYGNYAEAHDIGVRKRYKVAGQDMEVT 329
Query: 390 YFHTFVDGVDFVFIEAPMFRHL-NDIYGGSRLDILKRMILFCKAAVEVPWYVPCGGACYG 449
YFHT++DGVD VFI++P+FR+L ++IYGG+RLDIL+RM+LFCKAAVEVPW+VPCGG CYG
Sbjct: 330 YFHTYIDGVDIVFIDSPIFRNLESNIYGGNRLDILRRMVLFCKAAVEVPWHVPCGGICYG 389
Query: 450 DGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAHQGRGPVDDFSYVDLPP 509
DGNLVFIANDWHTALLPVYLKAYYRD+GLM YTRSVLVIHNIAHQGRGPV+DF+ VDL
Sbjct: 390 DGNLVFIANDWHTALLPVYLKAYYRDHGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSG 449
Query: 510 HYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQEGGWGLNGIINENDWK 569
+Y+D FKMYDPVGGEHFNIFAAGLK ADRIVTVSHGYAWELKT EGGWGL+ IINE+DWK
Sbjct: 450 NYLDLFKMYDPVGGEHFNIFAAGLKTADRIVTVSHGYAWELKTSEGGWGLHNIINESDWK 509
Query: 570 LRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEALQKELGLPVRDDVPLI 629
RGIVNG+D +WNP+ D +L SDGYTNY+L+ L+ GK QCK ALQ+ELGLPVR+DVP+I
Sbjct: 510 FRGIVNGVDTKDWNPQFDAYLTSDGYTNYNLKTLQTGKRQCKAALQRELGLPVREDVPII 569
Query: 630 GFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLRHFEGQYPDKVRGWVGF 689
FIGRLD QKGVD+IAE+IPW+M DVQLVMLGTGR DLEQML+ FE Q+ DK+R WVGF
Sbjct: 570 SFIGRLDHQKGVDLIAEAIPWMMSHDVQLVMLGTGRADLEQMLKEFEAQHCDKIRSWVGF 629
Query: 690 SVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLKDTVQPFDPYNNSG 749
SV+ AHRITAG DILLMPSRFEPCGLNQLYAM+YGT+PVVH VGGL+DTVQPF+P++ SG
Sbjct: 630 SVKMAHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVGGLRDTVQPFNPFDESG 689
Query: 750 LGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLSWDHAAEIYEEVLVAAK 800
+GWTFDRAEA++L+ AL NCLLTY+ YK SWEG+Q RGMSQDLSWD+AA+ YEEVLVAAK
Sbjct: 690 VGWTFDRAEANKLMAALWNCLLTYKDYKKSWEGIQERGMSQDLSWDNAAQQYEEVLVAAK 749
BLAST of Spo12258.1 vs. ExPASy Swiss-Prot
Match:
SSY22_ORYSJ (Soluble starch synthase 2-2, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica GN=SSII-2 PE=2 SV=2)
HSP 1 Score: 860.9 bits (2223), Expect = 1.100e-248
Identity = 390/505 (77.23%), Postives = 445/505 (88.12%), Query Frame = 1
Query: 296 EDAKPPPLAGANVMNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDY 355
E+ + PLAG NVMNV++VA+EC+P+ KTGGLGDV G+LPKALARRGHRVMVV PRYG+Y
Sbjct: 190 ENKESGPLAGPNVMNVIVVASECSPFCKTGGLGDVVGALPKALARRGHRVMVVIPRYGEY 249
Query: 356 ADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVDFVFIEAPMFRHL-NDIYGGSRLDILKRM 415
A+ +D GVRKRYRV GQD EV YFH F+DGVDFVF+EAP FRH NDIYGG R D+LKRM
Sbjct: 250 AEAKDLGVRKRYRVAGQDSEVSYFHAFIDGVDFVFLEAPPFRHRHNDIYGGERFDVLKRM 309
Query: 416 ILFCKAAVEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLV 475
ILFCKAAVEVPW+ PCGG+ YGDGNLVFIANDWHTALLPVYLKAYYRDNGLM YTRSVLV
Sbjct: 310 ILFCKAAVEVPWFAPCGGSIYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMQYTRSVLV 369
Query: 476 IHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYA 535
IHNIAHQGRGPVDDF+ +DLP HYID+F++YDPVGGEH N+FAAGLK ADR VTVSHGY
Sbjct: 370 IHNIAHQGRGPVDDFATMDLPEHYIDHFRLYDPVGGEHSNVFAAGLKMADRAVTVSHGYL 429
Query: 536 WELKTQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGK 595
WE+KT +GGWGL+ IIN NDWKL+GIVNGID+ EWNP++D HL SDGY NY+ E L GK
Sbjct: 430 WEIKTMDGGWGLHEIINHNDWKLQGIVNGIDMAEWNPEVDEHLQSDGYANYTFETLDTGK 489
Query: 596 SQCKEALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQD 655
QCKEALQ++LGL VRDDVPLIGFIGRLD QKGVDII +++PW+ G DVQ+VMLGTGR D
Sbjct: 490 KQCKEALQRQLGLQVRDDVPLIGFIGRLDHQKGVDIIGDAMPWIAGQDVQVVMLGTGRPD 549
Query: 656 LEQMLRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIP 715
LE+MLR FE ++ DKVRGWVGFSV+ AHRITAG D+LLMPSRFEPCGLNQLYAM YGT+P
Sbjct: 550 LEEMLRRFESEHNDKVRGWVGFSVQLAHRITAGADVLLMPSRFEPCGLNQLYAMAYGTVP 609
Query: 716 VVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRG 775
VVHAVGGL+DTV PFDP+ ++GLGWTFDRAEA+R++ ALG+CL TYR YK+SW GLQ RG
Sbjct: 610 VVHAVGGLRDTVAPFDPFADTGLGWTFDRAEANRMIDALGHCLNTYRNYKESWRGLQARG 669
Query: 776 MSQDLSWDHAAEIYEEVLVAAKYHW 800
M+QDLSWDHAAE+YE+VLV AKY W
Sbjct: 670 MAQDLSWDHAAELYEDVLVKAKYQW 694
BLAST of Spo12258.1 vs. ExPASy Swiss-Prot
Match:
SSY23_ORYSI (Soluble starch synthase 2-3, chloroplastic/amyloplastic OS=Oryza sativa subsp. indica GN=SSII-3 PE=1 SV=1)
HSP 1 Score: 839.3 bits (2167), Expect = 3.400e-242
Identity = 397/595 (66.72%), Postives = 471/595 (79.16%), Query Frame = 1
Query: 220 APEKPPSKSDGRSS-------------NEKRETIESLSRERTSYGLNTPKKTEEN-SMQP 279
AP PP S+ RSS ++ + +ES + + + P EE +
Sbjct: 216 APAPPPPGSNPRSSAPLPKPDNSEFAEDKSAKVVESAPKPKATRSSPIPAVEEETWDFKK 275
Query: 280 TFLSTTYIPSSIKDEQEQLEVGEQVNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTG 339
F + D+ + + + + +D PLAG NVMNV++VAAEC+PW KTG
Sbjct: 276 YFDLNEPDAAEDGDDDDDWADSDASDSEIDQDDDSGPLAGENVMNVIVVAAECSPWCKTG 335
Query: 340 GLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDG 399
GLGDVAG+LPKALARRGHRVMVV PRYGDYA+ QD G+RK Y+ GQD+EV YFH F+DG
Sbjct: 336 GLGDVAGALPKALARRGHRVMVVVPRYGDYAEAQDVGIRKYYKAAGQDLEVKYFHAFIDG 395
Query: 400 VDFVFIEAPMFRHL-NDIYGGSRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIA 459
VDFVFI+AP+FRH +DIYGG+R +I+KRMILFCKAAVEVPW+VPCGG YGDGNLVF+A
Sbjct: 396 VDFVFIDAPLFRHRQDDIYGGNRQEIMKRMILFCKAAVEVPWHVPCGGVPYGDGNLVFLA 455
Query: 460 NDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKM 519
NDWHTALLPVYLKAYYRDNG+M YTRSVLVIHNIA+QGRGPVD+F Y++LP HY+D+FK+
Sbjct: 456 NDWHTALLPVYLKAYYRDNGMMQYTRSVLVIHNIAYQGRGPVDEFPYMELPEHYLDHFKL 515
Query: 520 YDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGI 579
YDPVGGEH NIF AGLK ADR+VTVS GY WELKT EGGWGL+ II ENDWK+ GIVNGI
Sbjct: 516 YDPVGGEHANIFGAGLKMADRVVTVSPGYLWELKTTEGGWGLHDIIRENDWKMNGIVNGI 575
Query: 580 DVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDD 639
D EWNP++D HL SDGY NY++ +L GK +CK ALQ+ELGL VRDDVPLIGFIGRLD
Sbjct: 576 DYREWNPEVDVHLQSDGYANYTVASLDSGKPRCKAALQRELGLEVRDDVPLIGFIGRLDG 635
Query: 640 QKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRI 699
QKGVDII +++PW+ G DVQLV+LG+GR+DLE ML+ FE Q+ KVRGWVGFSV+ AHRI
Sbjct: 636 QKGVDIIGDAMPWIAGQDVQLVLLGSGRRDLEVMLQRFEAQHNSKVRGWVGFSVKMAHRI 695
Query: 700 TAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRA 759
TAG D+L+MPSRFEPCGLNQLYAM YGT+PVVHAVGGL+DTV FDP+ ++GLGWTFDRA
Sbjct: 696 TAGADVLVMPSRFEPCGLNQLYAMAYGTVPVVHAVGGLRDTVSAFDPFEDTGLGWTFDRA 755
Query: 760 EAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
E H+L+ ALG+CL TYR+YK+SW GLQ RGMSQDLSWDHAAE+YEEVLV AKY W
Sbjct: 756 EPHKLIEALGHCLETYRKYKESWRGLQVRGMSQDLSWDHAAELYEEVLVKAKYQW 810
BLAST of Spo12258.1 vs. TAIR (Arabidopsis)
Match:
AT3G01180.1 (starch synthase 2)
HSP 1 Score: 944.1 bits (2439), Expect = 5.500e-275
Identity = 491/773 (63.52%), Postives = 585/773 (75.68%), Query Frame = 1
Query: 45 HSGDFAHGRSSLCCVKSVGGCRRGYSWRVG--CSKEGTNDSSDSEDSKETDDAVQATIEK 104
+S H R S + S R S+ +G C ++S S+D E +DA+QATI+K
Sbjct: 22 NSSTLRHSRVSYHDLPSGSLSFRSRSFVLGHRCKCVSRVEASGSDDD-EPEDALQATIDK 81
Query: 105 SNKVLAMQKELLQQIAQRKKLVSSIKSSIINHENEPFMRENDNSPPKVDFSTNQTR--VN 164
S KVLAMQ+ LL QIA+R+KLVSSIK S + ++ + + S V+ +T+ T+ +
Sbjct: 82 SKKVLAMQRNLLHQIAERRKLVSSIKESTPDLDDAKASSKQE-SASSVNANTDATKKEIM 141
Query: 165 NEHTSGTVSSRNIDSFS-SKVSKGQVPIPVNSREGRSGQGKLSLGTLNLGSSMQKEDLTR 224
+ +G+VS S SK + + P Q S+ + + +S QK
Sbjct: 142 DGDANGSVSPSTYGKSSLSKEPEAKTFSPSTESLKNRKQSSASVISSSPVTSPQKPSDVA 201
Query: 225 APEKPPSKSDGRSSNEKRETIESL-SRERTSYGLNTPKKTEENSMQ-------PTFLSTT 284
KP S S + + + S E+TS + +P K ++ P++L+
Sbjct: 202 TNGKPWSSVVASSVDPPYKPSSVMTSPEKTSDPVTSPGKPSKSRAGAFWSDPLPSYLTKA 261
Query: 285 YIPSSIKDEQEQLEVGE----QVNHPVREDAKPPPLAGANVMNVVLVAAECAPWRKTGGL 344
S++K E+ + + + N P +++ KPPPLAGANVMNV+LVAAECAP+ KTGGL
Sbjct: 262 PQTSTMKTEKYVEKTPDVASSETNEPGKDEEKPPPLAGANVMNVILVAAECAPFSKTGGL 321
Query: 345 GDVAGSLPKALARRGHRVMVVTPRYGDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVD 404
GDVAG+LPK+LARRGHRVMVV PRY +YA+ +D GVRKRY+V GQD+EV YFH F+DGVD
Sbjct: 322 GDVAGALPKSLARRGHRVMVVVPRYAEYAEAKDLGVRKRYKVAGQDMEVMYFHAFIDGVD 381
Query: 405 FVFIEAPMFRHL-NDIYGGSRLDILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIAND 464
FVFI++P FRHL N+IYGG+RLDILKRM+LFCKAAVEVPWYVPCGG CYGDGNL FIAND
Sbjct: 382 FVFIDSPEFRHLSNNIYGGNRLDILKRMVLFCKAAVEVPWYVPCGGVCYGDGNLAFIAND 441
Query: 465 WHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYD 524
WHTALLPVYLKAYYRD+G+M YTRSVLVIHNIAHQGRGPVDDFSYVDLP HY+D FK+YD
Sbjct: 442 WHTALLPVYLKAYYRDHGIMKYTRSVLVIHNIAHQGRGPVDDFSYVDLPSHYLDSFKLYD 501
Query: 525 PVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDV 584
PVGGEHFNIFAAGLKAADR++TVSHGY+WE+KT EGGWGL+ IINENDWK RGIVNGID
Sbjct: 502 PVGGEHFNIFAAGLKAADRVLTVSHGYSWEVKTLEGGWGLHNIINENDWKFRGIVNGIDT 561
Query: 585 TEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQK 644
EWNP+ D +LHSD YTNYSLENL GK QCK ALQKELGLPVR DVPLIGFIGRLD QK
Sbjct: 562 QEWNPEFDTYLHSDDYTNYSLENLHIGKPQCKAALQKELGLPVRPDVPLIGFIGRLDHQK 621
Query: 645 GVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITA 704
GVD+IAE++PW+M DVQLVMLGTGR DLE++LR E QY DK RGWVGFSV+TAHRITA
Sbjct: 622 GVDLIAEAVPWMMSQDVQLVMLGTGRPDLEEVLRQMEHQYRDKARGWVGFSVKTAHRITA 681
Query: 705 GVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGLGWTFDRAEA 764
G DILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGL+DTVQ FDPY+ +GLGWTFD AEA
Sbjct: 682 GADILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLRDTVQQFDPYSETGLGWTFDSAEA 741
Query: 765 HRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLSWDHAAEIYEEVLVAAKYHW 800
+L+HALGNCLLTYR+YK+SWEGLQRRGM+QDLSWD+AAE YEEVLVAAKYHW
Sbjct: 742 GKLIHALGNCLLTYREYKESWEGLQRRGMTQDLSWDNAAEKYEEVLVAAKYHW 792
BLAST of Spo12258.1 vs. TAIR (Arabidopsis)
Match:
AT5G24300.1 (Glycogen/starch synthases, ADP-glucose type)
HSP 1 Score: 468.8 bits (1205), Expect = 6.800e-132
Identity = 264/597 (44.22%), Postives = 347/597 (58.12%), Query Frame = 1
Query: 229 DGRSSNEKRETIESLSRERTSYGLNTPKKTEENSMQPTFLSTTYIPSSIKDEQEQLEVGE 288
D R S+E+R ++ ER G + Q T ++T I +E+ E +
Sbjct: 56 DSRESDEERFITDA---ERDGSGSVLGFQLTPPGDQQTVSTST---GEITHHEEKKEAID 115
Query: 289 QV---------NHPVREDAKPPPLAGAN---VMNVVLVAAECAPWRKTGGLGDVAGSLPK 348
Q+ N V E A + V N+V V +E AP+ KTGGLGDV GSLP
Sbjct: 116 QIVMADFGVPGNRAVEEGAAEVGIPSGKAEVVNNLVFVTSEAAPYSKTGGLGDVCGSLPI 175
Query: 349 ALARRGHRVMVVTPRY-------GDYADIQDSGVRKRYRVEGQDIEVGYFHTFVDGVDFV 408
ALA RGHRVMV++PRY +YA +D G+R G EV ++H + DGVD+V
Sbjct: 176 ALAGRGHRVMVISPRYLNGTAADKNYARAKDLGIRVTVNCFGGSQEVSFYHEYRDGVDWV 235
Query: 409 FIEAPMFRHLNDIYGGSRL---DILKRMILFCKAAVEVPWYVPCGGACYGDGNLVFIAND 468
F++ + + YG S+ D R L C AA E P +P GG YG+ +L F+ ND
Sbjct: 236 FVDHKSYHRPGNPYGDSKGAFGDNQFRFTLLCHAACEAPLVLPLGGFTYGEKSL-FLVND 295
Query: 469 WHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYD 528
WH L+P+ L A YR G+ RS+L+IHN+AHQG P ++ + LP +
Sbjct: 296 WHAGLVPILLAAKYRPYGVYKDARSILIIHNLAHQGVEPAATYTNLGLPSEWYGAVGWVF 355
Query: 529 PVG--------GEHFNIFAAGLKAADRIVTVSHGYAWELKTQEGGWGLNGIINENDWKLR 588
P GE N+ + +DRI+TVS GYAWE+ T EGG+GL +++ +
Sbjct: 356 PTWARTHALDTGEAVNVLKGAIVTSDRIITVSQGYAWEITTVEGGYGLQDLLSSRKSVIN 415
Query: 589 GIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEALQKELGLPVRDDVPLIGF 648
GI NGI+V EWNP D H+ +YS +++ K +CK ALQKELGLP+R + P+IGF
Sbjct: 416 GITNGINVDEWNPSTDEHIPF----HYSADDVSE-KIKCKMALQKELGLPIRPECPMIGF 475
Query: 649 IGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQMLRHFEGQYPDKVRGWVGFSV 708
IGRLD QKG+D+I + P LM D+Q VMLG+G E +R E Y DK RGWVGF+V
Sbjct: 476 IGRLDYQKGIDLIQTAGPDLMVDDIQFVMLGSGDPKYESWMRSMEETYRDKFRGWVGFNV 535
Query: 709 RTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLKDTVQPFDPY----NN 768
+HRITAG DILLMPSRFEPCGLNQLYAM YGTIPVVH GGL+DTV+ F+PY
Sbjct: 536 PISHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPVVHGTGGLRDTVENFNPYAEGGAG 595
Query: 769 SGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLSWDHAAEIYEEV 792
+G GW F +V AL TYR+YK SWEGL RRGM+++ SW++AA YE+V
Sbjct: 596 TGTGWVFTPLSKDSMVSALRLAAATYREYKQSWEGLMRRGMTRNYSWENAAVQYEQV 640
BLAST of Spo12258.1 vs. TAIR (Arabidopsis)
Match:
AT1G32900.1 (UDP-Glycosyltransferase superfamily protein)
HSP 1 Score: 369.8 bits (948), Expect = 4.300e-102
Identity = 208/511 (40.70%), Postives = 290/511 (56.75%), Query Frame = 1
Query: 309 MNVVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYGDYADIQDSGVRKRYR 368
M+V+ + AE PW KTGGLGDV G LP ALA RGHRVM + PRY Y D D+ V + +
Sbjct: 84 MSVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTICPRYDQYKDAWDTCVVVQIK 143
Query: 369 VEGQDIEVGYFHTFVDGVDFVFIEAPMF----------RHLNDIYGGSRLDILKRMILFC 428
V + V +FH + GVD VF++ P+F + I G D R L C
Sbjct: 144 VGDKVENVRFFHCYKRGVDRVFVDHPIFLAKVVGKTGSKIYGPITGVDYNDNQLRFSLLC 203
Query: 429 KAAVEVPWYVPCGGACYGDG----NLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLV 488
+AA+E P + + Y G ++VF+ANDWHTALLP YLK+ Y+ G+ M + V
Sbjct: 204 QAALEAPQVLNLNSSKYFSGPYGEDVVFVANDWHTALLPCYLKSMYQSRGVYMNAKVVFC 263
Query: 489 IHNIAHQGRGPVDDFSYVDLPPHYIDYFKMYD----PVGGEHFNIFAAGLKAADRIVTVS 548
IHNIA+QGR DD+S ++LP + F D PV G N A + A R++TVS
Sbjct: 264 IHNIAYQGRFAFDDYSLLNLPISFKSSFDFMDGYEKPVKGRKINWMKAAILEAHRVLTVS 323
Query: 549 HGYAWELKTQ-EGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLEN 608
YA EL + + G L+ + + GI+NG+DV EWNP D+++ Y +
Sbjct: 324 PYYAQELISGVDRGVELHKYLRMKT--VSGIINGMDVQEWNPSTDKYID----IKYDITT 383
Query: 609 LKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLG 668
+ K KEALQ +GLPV DVP+IGFIGRL++QKG DI+ E+I MGL+VQ+V+LG
Sbjct: 384 VTDAKPLIKEALQAAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAISKFMGLNVQMVILG 443
Query: 669 TGRQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMN 728
TG++ +E + E ++P K G F+V AH ITAG D +++PSRFEPCGL QL+AM
Sbjct: 444 TGKKKMEAQILELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSRFEPCGLIQLHAMR 503
Query: 729 YGTIPVVHAVGGLKDTVQPFDPYNNSGLG------WTFDRAEAHRLVHALGNCLLTYRQY 788
YGT+P+V + GGL DTV+ D Y +G D + A+ + Y
Sbjct: 504 YGTVPIVASTGGLVDTVK--DGYTGFHIGRFNVKCEVVDPDDVIATAKAVTRAVAVYG-- 563
Query: 789 KDSWEGLQRRGMSQDLSWDHAAEIYEEVLVA 795
+ + + + M QD SW A ++E+VL++
Sbjct: 564 TSAMQEMVKNCMDQDFSWKGPARLWEKVLLS 584
BLAST of Spo12258.1 vs. TAIR (Arabidopsis)
Match:
AT4G18240.1 (starch synthase 4)
HSP 1 Score: 223.0 bits (567), Expect = 6.500e-58
Identity = 164/503 (32.60%), Postives = 242/503 (48.11%), Query Frame = 1
Query: 311 VVLVAAECAPWRKTGGLGDVAGSLPKALARRGHRVMVVTPRYG--DYADIQD----SGVR 370
VV +AAE AP K GGLGDV L KAL R+GH V ++ P+Y Y ++D V
Sbjct: 544 VVHIAAEMAPVAKVGGLGDVVAGLGKALQRKGHLVEIILPKYDCMQYDRVRDLRALDTVV 603
Query: 371 KRYRVEGQDIEVGYFHTFVDGVDFVFIEAP---MFRHLNDIYGGSRLDILKRMILFCKAA 430
+ Y +G+ + + V+G+ FIE F YG D +R F +AA
Sbjct: 604 ESY-FDGKLYKNKIWIGTVEGLPVHFIEPQHPSKFFWRGQFYGEQ--DDFRRFSYFSRAA 663
Query: 431 VEVPWYVPCGGACYGDGNLVFIANDWHTALLPVYLKAYYRDNGLMMYTRSVLVIHNIAHQ 490
+E+ G + +DW TA + Y GL R HN +Q
Sbjct: 664 LELLLQS-------GKKPDIIHCHDWQTAFVAPLYWDLYAPKGLDS-ARICFTCHNFEYQ 723
Query: 491 GRGPVDDFSYVDLPPHYIDY-FKMYDPVGGEHFNIFAAGLKAADRIVTVSHGYAWELKTQ 550
G + L + ++ +M D G+ N + ++ + TVS YA E++T
Sbjct: 724 GTASASELGSCGLDVNQLNRPDRMQDHSSGDRVNPVKGAIIFSNIVTTVSPTYAQEVRTA 783
Query: 551 EGGWGLNGIINENDWKLRGIVNGIDVTEWNPKLDRHLHSDGYTNYSLENLKPGKSQCKEA 610
EGG GL+ +N + K GI+NGID WNP D L + ++ ++L+ GK + K A
Sbjct: 784 EGGKGLHSTLNFHSKKFIGILNGIDTDSWNPATDPFLKAQ----FNAKDLQ-GKEENKHA 843
Query: 611 LQKELGLPVRDDV-PLIGFIGRLDDQKGVDIIAESIPWLMGLDVQLVMLGTGRQDLEQML 670
L+K+LGL + PL+G I RL QKGV +I +I + L Q V+LG+ + +
Sbjct: 844 LRKQLGLSSAESRRPLVGCITRLVPQKGVHLIRHAIYRTLELGGQFVLLGSS--PVPHIQ 903
Query: 671 RHFEG-----QYPDKVRGWVGFSVRTAHRITAGVDILLMPSRFEPCGLNQLYAMNYGTIP 730
R FEG + D VR + + +H I A D+ ++PS FEPCGL Q+ AM YG+IP
Sbjct: 904 REFEGIEQQFKSHDHVRLLLKYDEALSHTIYAASDLFIIPSIFEPCGLTQMIAMRYGSIP 963
Query: 731 VVHAVGGLKDTVQPFD------PYNNSGLGWTFDRAEAHRLVHALGNCLLTYRQYKDSWE 790
+ GGL D+V D + N G+TF A+ +AL Y++ ++ W
Sbjct: 964 IARKTGGLNDSVFDIDDDTIPTQFQN---GFTFQTADEQGFNYALERAFNHYKKDEEKWM 1023
Query: 791 GLQRRGMSQDLSWDHAAEIYEEV 792
L + MS D SW +A YEE+
Sbjct: 1024 RLVEKVMSIDFSWGSSATQYEEL 1025
BLAST of Spo12258.1 vs. TAIR (Arabidopsis)
Match:
AT1G11720.2 (starch synthase 3)
HSP 1 Score: 156.4 bits (394), Expect = 7.500e-38
Identity = 98/295 (33.22%), Postives = 145/295 (49.15%), Query Frame = 1
Query: 514 NIFAAGLKAADRIVTVSHGYAWELKTQEGGWGLNGIINENDWKLRGIVNGIDVTEWNPKL 573
N + AD+ TVS YA E+ N +I+ + +K GI+NGID W+P
Sbjct: 811 NAIGKAMTFADKATTVSPTYAKEVAG-------NSVISAHLYKFHGIINGIDPDIWDPYN 870
Query: 574 DRHLHSDGYTNYSLENLKPGKSQCKEALQKELGLPVRDDVPLIGFIGRLDDQKGVDIIAE 633
D + Y+ EN+ GK KE LQ LGL D P++G I RL QKG+ +I
Sbjct: 871 DNFIP----VPYTSENVVEGKRAAKEELQNRLGLKSAD-FPVVGIITRLTHQKGIHLIKH 930
Query: 634 SIPWLMGLDVQLVMLGTG-----RQDLEQMLRHFEGQYPDKVRGWVGFSVRTAHRITAGV 693
+I + + Q+V+LG+ + D + + D+ R + + +H I AG
Sbjct: 931 AIWRTLERNGQVVLLGSAPDPRIQNDFVNLANQLHSSHGDRARLVLTYDEPLSHLIYAGA 990
Query: 694 DILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLKDTVQPFDPYNNSGL-------GWTF 753
D +L+PS FEPCGL QL AM YG +PVV GGL DTV D G++F
Sbjct: 991 DFILVPSIFEPCGLTQLIAMRYGAVPVVRKTGGLFDTVFDVDHDKERAQAQVLEPNGFSF 1050
Query: 754 DRAEAHRLVHALGNCLLTYRQYKDSWEGLQRRGMSQDLSWDHAAEIYEEVLVAAK 797
D A+A + +AL + + ++ + L + M QD SW+ A Y E+ +A+
Sbjct: 1051 DGADAPGVDYALNRAISAWYDGREWFNSLCKTVMEQDWSWNRPALEYLELYHSAR 1093
The following BLAST results are available for this feature:
