Spo11204 (gene)

Overview
NameSpo11204
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionDNA-directed RNA polymerase subunit beta
LocationSuper_scaffold_134 : 283814 .. 284819 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGATATGGCCATATGCCCATATGTCACTTCTTTCGGCCGGGTTGAATAAATACGGGTCAAAAACATATGTACATACGGAGTATTATGCAACCAAACCCCCAAAATCACAGATTTTGGATAGCTGCCACAAGATAAGACCATGCTGCTCTCAGGGAAGAGCTTCTTGTGCACCAAAATTTCCCCCCATTTCTACAGAAATAAAAGCAAAAGATTCACAAAAATATTTGCAAAAAAGCCAGAATTTCCTGAATTCCCAAACAAAAACCAAAAATCCAATTTTGAATTCAGGGTCACCAACAACATTTTAGCTCAATCTGCCATTGCAATCCTGGGTTTAGGTTTTGTTGATGCAGGGTAATAATCCATTTCCAGTTTTCCACCACAAAATTTCTTAACTTTACAATATATTGCTGTTATAATTTTTATTGATTTGTGATTGTTATTTGAAACATTGAATTGGGTTCAGATATAGTGGAGATTGGTCAAGAATTGGAGTGATCTCAAAACAAACTGAGGATGTGCTGAAGATTGGAGCTTTTTTGGTGGTTCCACTCTGTTTGTTTGTTGTTTTATCTCTTGCTAAAAAACCAGAAGAATAACGTATATAGAAGATCCTGTATTTTGTGTCATTGACTCATTGTGTATTTGTGTGTGTTATTTTACAGTTTAACGACTGTTTTAAGTGATTATTAGAATGATCGAGTTTAATGTTAATTTTTTAATTAAGGAGTTCTATAAAACCGCGTTCAATAAGATAGGCACTAGTGGTTATCAACACCAAATTTGATTTGATAACCAGGTGTTGAAGAAGAAGTGTAAGGGTAAGAGCGTCGACGAGATTGTGAAGGCACGTAACCTTGATGTATTGACACATTTACAAAAGTTGCGCCAACGTAAATTGTTTAATTGTTAACTTTTAACTTCAGTGTATTATAATAAATCAGCCAAATGGATAATCTGATAAATTACCATGTTTTTTTTAGGAGTAGTGATATACCACTACCAG

mRNA sequence

TGATATGGCCATATGCCCATATGTCACTTCTTTCGGCCGGGTTGAATAAATACGGGTCAAAAACATATGTACATACGGAGTATTATGCAACCAAACCCCCAAAATCACAGATTTTGGATAGCTGCCACAAGATAAGACCATGCTGCTCTCAGGGAAGAGCTTCTTGTGCACCAAAATTTCCCCCCATTTCTACAGAAATAAAAGCAAAAGATTCACAAAAATATTTGCAAAAAAGCCAGAATTTCCTGAATTCCCAAACAAAAACCAAAAATCCAATTTTGAATTCAGGGTCACCAACAACATTTTAGCTCAATCTGCCATTGCAATCCTGGGTTTAGGTTTTGTTGATGCAGGATATAGTGGAGATTGGTCAAGAATTGGAGTGATCTCAAAACAAACTGAGGATGTGCTGAAGATTGGAGCTTTTTTGGTGGTTCCACTCTGTTTGTTTGTTGTTTTATCTCTTGCTAAAAAACCAGAAGAATAACGTATATAGAAGATCCTGTATTTTGTGTCATTGACTCATTGTGTATTTGTGTGTGTTATTTTACAGTTTAACGACTGTTTTAAGTGATTATTAGAATGATCGAGTTTAATGTTAATTTTTTAATTAAGGAGTTCTATAAAACCGCGTTCAATAAGATAGGCACTAGTGGTTATCAACACCAAATTTGATTTGATAACCAGGTGTTGAAGAAGAAGTGTAAGGGTAAGAGCGTCGACGAGATTGTGAAGGCACGTAACCTTGATGTATTGACACATTTACAAAAGTTGCGCCAACGTAAATTGTTTAATTGTTAACTTTTAACTTCAGTGTATTATAATAAATCAGCCAAATGGATAATCTGATAAATTACCATGTTTTTTTTAGGAGTAGTGATATACCACTACCAG

Coding sequence (CDS)

ATGCTGCTCTCAGGGAAGAGCTTCTTGTGCACCAAAATTTCCCCCCATTTCTACAGAAATAAAAGCAAAAGATTCACAAAAATATTTGCAAAAAAGCCAGAATTTCCTGAATTCCCAAACAAAAACCAAAAATCCAATTTTGAATTCAGGGTCACCAACAACATTTTAGCTCAATCTGCCATTGCAATCCTGGGTTTAGGTTTTGTTGATGCAGGATATAGTGGAGATTGGTCAAGAATTGGAGTGATCTCAAAACAAACTGAGGATGTGCTGAAGATTGGAGCTTTTTTGGTGGTTCCACTCTGTTTGTTTGTTGTTTTATCTCTTGCTAAAAAACCAGAAGAATAA

Protein sequence

MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSAIAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo11204.1Spo11204.1mRNA


Homology
[Close]
BLAST of Spo11204.1 vs. NCBI nr
Match: gi|902232984|gb|KNA22883.1| (hypothetical protein SOVF_030160 [Spinacia oleracea])

HSP 1 Score: 230.3 bits (586), Expect = 1.700e-57
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSA 60
           MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSA
Sbjct: 1   MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSA 60

Query: 61  IAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE 116
           IAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE
Sbjct: 61  IAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE 115
[Close]
BLAST of Spo11204.1 vs. NCBI nr
Match: gi|731327161|ref|XP_010674382.1| (PREDICTED: uncharacterized protein LOC104890550 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 165.2 bits (417), Expect = 6.600e-38
Identity = 81/115 (70.43%), Postives = 93/115 (80.87%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSA 60
           MLLSG  F+ TK S HF RNK KR T IFAKK EF  FP+   K NFE +++N I+AQSA
Sbjct: 1   MLLSGMDFMPTKTSHHFLRNKGKRSTAIFAKKQEFSNFPDNKDKPNFELKISNKIIAQSA 60

Query: 61  IAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE 116
           IAILGLGFVDAGYSGDWSRIGVIS++TEDVLKI AFLVVP CLFV+ SLA+ P++
Sbjct: 61  IAILGLGFVDAGYSGDWSRIGVISRETEDVLKIAAFLVVPFCLFVIFSLARNPDK 115
[Close]
BLAST of Spo11204.1 vs. NCBI nr
Match: gi|703155127|ref|XP_010111121.1| (hypothetical protein L484_008498 [Morus notabilis])

HSP 1 Score: 116.3 bits (290), Expect = 3.500e-23
Identity = 57/115 (49.57%), Postives = 81/115 (70.43%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLCTKISPHFYRNKSKR-FTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQS 60
           M+++ KSF+   IS   +  K+ R F    AKK + PE     Q+S    R+++  L QS
Sbjct: 1   MVVAEKSFILGNISSSLFAKKNGRSFIITLAKKKDLPETSKTQQRSILPLRISSTFLGQS 60

Query: 61  AIAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPE 115
            IA+ GLGF+DAGYSGDWSRIGVISK++ED++K+ AF+VVPLCLF+++ L+K+ E
Sbjct: 61  GIAVFGLGFIDAGYSGDWSRIGVISKESEDLIKVAAFIVVPLCLFLIVKLSKERE 115
[Close]
BLAST of Spo11204.1 vs. NCBI nr
Match: gi|1009109734|ref|XP_015891804.1| (PREDICTED: uncharacterized protein LOC107426201 [Ziziphus jujuba])

HSP 1 Score: 114.4 bits (285), Expect = 1.300e-22
Identity = 62/117 (52.99%), Postives = 82/117 (70.09%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLC---TKISPH--FYRNKSKRFTKIFAKKPEFPEFPNKN-QKSNFEFRVTNN 60
           M+++  SF+    TK+ P   F R   ++F    AKK + PE      QK +F FRV+N 
Sbjct: 1   MVVTENSFISATITKLLPSHLFVRTNGRKFGTTLAKKRDLPETSKTQIQKPSFPFRVSNR 60

Query: 61  ILAQSAIAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAK 112
           +LAQSAI + GLGF+DAGYSGDWSRIGVISK++E++LKI AF+V PLCLF+V  L+K
Sbjct: 61  VLAQSAIGLFGLGFIDAGYSGDWSRIGVISKESEELLKIAAFVVAPLCLFLVFYLSK 117
[Close]
BLAST of Spo11204.1 vs. NCBI nr
Match: gi|225445770|ref|XP_002274551.1| (PREDICTED: uncharacterized protein LOC100267275 [Vitis vinifera])

HSP 1 Score: 114.0 bits (284), Expect = 1.700e-22
Identity = 58/116 (50.00%), Postives = 82/116 (70.69%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLCTKISPHFY--RNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQ 60
           ML++  +F+   + P+ +  +N +K    +  K+        + Q+S    RV+N ILA+
Sbjct: 1   MLVTQDTFIFNTLPPYLFHIKNGTKFRPTLAQKRDASKNSSTQQQQSILPLRVSNTILAR 60

Query: 61  SAIAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPE 115
           SAIA+LGLGF+DAGYSGDWSRIGVISK+TED LK+ A LVVPLCLF++ S++K+PE
Sbjct: 61  SAIAVLGLGFIDAGYSGDWSRIGVISKETEDFLKLSALLVVPLCLFLIFSISKEPE 116
[Close]
BLAST of Spo11204.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RTV1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_030160 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 1.200e-57
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSA 60
           MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSA
Sbjct: 1   MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSA 60

Query: 61  IAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE 116
           IAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE
Sbjct: 61  IAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE 115
[Close]
BLAST of Spo11204.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CPS3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g078420 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 4.600e-38
Identity = 81/115 (70.43%), Postives = 93/115 (80.87%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLCTKISPHFYRNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSA 60
           MLLSG  F+ TK S HF RNK KR T IFAKK EF  FP+   K NFE +++N I+AQSA
Sbjct: 1   MLLSGMDFMPTKTSHHFLRNKGKRSTAIFAKKQEFSNFPDNKDKPNFELKISNKIIAQSA 60

Query: 61  IAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPEE 116
           IAILGLGFVDAGYSGDWSRIGVIS++TEDVLKI AFLVVP CLFV+ SLA+ P++
Sbjct: 61  IAILGLGFVDAGYSGDWSRIGVISRETEDVLKIAAFLVVPFCLFVIFSLARNPDK 115
[Close]
BLAST of Spo11204.1 vs. UniProtKB/TrEMBL
Match: W9S754_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_008498 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.400e-23
Identity = 57/115 (49.57%), Postives = 81/115 (70.43%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLCTKISPHFYRNKSKR-FTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQS 60
           M+++ KSF+   IS   +  K+ R F    AKK + PE     Q+S    R+++  L QS
Sbjct: 1   MVVAEKSFILGNISSSLFAKKNGRSFIITLAKKKDLPETSKTQQRSILPLRISSTFLGQS 60

Query: 61  AIAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPE 115
            IA+ GLGF+DAGYSGDWSRIGVISK++ED++K+ AF+VVPLCLF+++ L+K+ E
Sbjct: 61  GIAVFGLGFIDAGYSGDWSRIGVISKESEDLIKVAAFIVVPLCLFLIVKLSKERE 115
[Close]
BLAST of Spo11204.1 vs. UniProtKB/TrEMBL
Match: D7U1G5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0037g00830 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.200e-22
Identity = 58/116 (50.00%), Postives = 82/116 (70.69%), Query Frame = 1 

		  

Query: 1   MLLSGKSFLCTKISPHFY--RNKSKRFTKIFAKKPEFPEFPNKNQKSNFEFRVTNNILAQ 60
           ML++  +F+   + P+ +  +N +K    +  K+        + Q+S    RV+N ILA+
Sbjct: 1   MLVTQDTFIFNTLPPYLFHIKNGTKFRPTLAQKRDASKNSSTQQQQSILPLRVSNTILAR 60

Query: 61  SAIAILGLGFVDAGYSGDWSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPE 115
           SAIA+LGLGF+DAGYSGDWSRIGVISK+TED LK+ A LVVPLCLF++ S++K+PE
Sbjct: 61  SAIAVLGLGFIDAGYSGDWSRIGVISKETEDFLKLSALLVVPLCLFLIFSISKEPE 116
[Close]
BLAST of Spo11204.1 vs. UniProtKB/TrEMBL
Match: A0A067KUW6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04081 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 5.100e-21
Identity = 53/98 (54.08%), Postives = 75/98 (76.53%), Query Frame = 1 

		  

Query: 19  RNKSKRFTKIFAKKPEFPEFPNKNQKSN--FEFRVTNNILAQSAIAILGLGFVDAGYSGD 78
           +N  K F  +  K+ +  E  +K QK    F  RV+N+IL+QSA+A+ GLGF+DAGYSGD
Sbjct: 33  KNGQKYFATLAKKRDDLSE-DSKTQKKQPFFPLRVSNSILSQSAVAVFGLGFIDAGYSGD 92

Query: 79  WSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLAKKPE 115
           WSRIGVISK++ED+LKI AF+V+PLC+F+V S+++K +
Sbjct: 93  WSRIGVISKESEDLLKIAAFVVIPLCIFLVFSISRKEQ 129
[Close]
BLAST of Spo11204.1 vs. TAIR (Arabidopsis)
Match: AT4G30845.1 (unknown protein)

HSP 1 Score: 99.4 bits (246), Expect = 1.600e-21
Identity = 52/94 (55.32%), Postives = 70/94 (74.47%), Query Frame = 1 

		  

Query: 20  NKSKRFTK---IFAKKPEFPEFPNKNQKSNFEFRVTNNILAQSAIAILGLGFVDAGYSGD 79
           NK + F     I +K+ +F E  N+ ++     +V N I+A+SAIA+L LGF+DAGYSGD
Sbjct: 17  NKLRSFANGVTIMSKRRDFSEKSNE-ERPILRIKVPNTIVARSAIAVLSLGFIDAGYSGD 76

Query: 80  WSRIGVISKQTEDVLKIGAFLVVPLCLFVVLSLA 111
           WSRIGVISK+TE++LKI AFLVVPLC+F+ LS +
Sbjct: 77  WSRIGVISKETEELLKIAAFLVVPLCIFLALSFS 109
The following BLAST results are available for this feature:
BLAST of Spo11204.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902232984|gb|KNA22883.1|1.7e-57100.hypothetical protein SOVF_0301... [more]
gi|731327161|ref|XP_010674382.1|6.6e-3870.4PREDICTED: uncharacterized pro... [more]
gi|703155127|ref|XP_010111121.1|3.5e-2349.5hypothetical protein L484_0084... [more]
gi|1009109734|ref|XP_015891804.1|1.3e-2252.9PREDICTED: uncharacterized pro... [more]
gi|225445770|ref|XP_002274551.1|1.7e-2250.0PREDICTED: uncharacterized pro... [more]
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BLAST of Spo11204.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RTV1_SPIOL1.2e-57100.Uncharacterized protein OS=Spi... [more]
A0A0J8CPS3_BETVU4.6e-3870.4Uncharacterized protein OS=Bet... [more]
W9S754_9ROSA2.4e-2349.5Uncharacterized protein OS=Mor... [more]
D7U1G5_VITVI1.2e-2250.0Putative uncharacterized prote... [more]
A0A067KUW6_JATCU5.1e-2154.0Uncharacterized protein OS=Jat... [more]
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BLAST of Spo11204.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo11204.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT4G30845.11.6e-2155.3unknown protein[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR38389FAMILY NOT NAMEDcoord: 23..115
score: 1.3

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
molecular_function GO:0016887 ATPase activity
molecular_function GO:0005524 ATP binding
RNA-Seq Expression