Spo10197 (gene)

Overview
NameSpo10197
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionNitrate reductase
LocationSuper_scaffold_225 : 156701 .. 158246 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTTTTTTAATTTTATTTCCACAACTGGTTTAATTCAAAAATGCCATATGATTTACGCAAATGTCAGTCGTTGATTTTCATTTTCATTTTCATTTTCACCCACAGGACTGATACAAATCTTAAAGACTCTTGTGTCATTTACTCATTTACGATTATTGCGAAGCACCATCATAGATTTCTAATTTCTTGCGACGATTTTAATTTCTCACTGATCCTGCAATTACATCAGTTGAAGCTCCAAATTCTGGAAAATGGAGATGATTGTAGCAGGGTCATTGATACTGGGTCTTCTATTAGCTCTTCTCTTTGTCATCCCACGATTTACCAAACCCAGTTATCTTTCTCCATCCTTTTCTATACCCAATTCAAGATTTTGACTTTTTAGTGAAATTCTTCCATCTCAGTTATTATTACTGATTAATCGTCTGATTTGTGTTAACAATACAGGTCAATCGAAAACTGAAGCCTTGAATAATAACCCCAAAAACATATCTAAGGTAATACATTTGATTTTTGTGGTTTTTTTCGGTTTCTTCGCCTTTTTAAAAAATTTATTGGTAAAATGTTCTAAAGCTTCGATTATGCTCTAAATTAATGTGCTTTTATCATCTTTTTAGCATTAAAGTTTACTTGTATTGAATCTTTGCTGTGTAAGACTGTAGCTACCAATCTATCATGGATATTTGTAAACATATTGATTTCATACTGCGGAAGTGTTTTTTTATTTTCTATTAGCCTTGTACAGTTGCCTGGTACACCCAGGTTTATAATAGAAATTTCCGGGGGAGCTTAGTATTGCAGCTTCTGAAATGCTGAATGTAACCATTAACAATATCTGAAATATTTTTGTTTGCTTGATTGAAACTTAACTGCCTTGAGGAAGGAATTGCAGCTTCACTGCCTCGATCTTCCGACTTAATTGCCTATTTTAGTTTCTTTCATTGAAGGCATCTCAGAAGAGTGACTGAATGTCAATGCTGACATCAAATCTCGGGTTTAAAGGGTTTTGGGTGACTTGGCAGGATCTTTGTATATCATTTTTTATTGATATCCACTCATGGAAAATGTTATTAGGTTTGATCTCTGTAAATTAAAAAGAGATTAATTATCTTTGTTTCTTCTTTTGACAAGGAATCTAGAAGATATACTAAAGCTGAAGTTTCGTTGCATGACAAAAGGACTGATTGTTGGGTCATCATCAAAGATAAGGTTAAATTGTTTTGCCGTCCAATCTGTTTCTTCAGTGTGATCATTAACTTGAAAGAAAGAATTGCTAGTTCTATAACTGTGATGCTGTTAATGTAGGTATATGATGTAACTTCATATGTGGAGGAGCATCCAGGTGGTGATGCTATCTTAACGCATGCAGGGGATGATTCCACTGAAGGGTTCTTTGGGTATGCACCTTCCATATCCGTTGCATTTGTTGCTATTAGAGGAATACACTTCCCATTACTTCTCTGGAAAATGTTATTGTGGCCTTTTCAGAAAATGTTATCCGTTGCATTTGTTCCGGGGTTGAAACGATGGAAGGAGCGTCCTTGA

mRNA sequence

GATTTTTTTAATTTTATTTCCACAACTGGTTTAATTCAAAAATGCCATATGATTTACGCAAATGTCAGTCGTTGATTTTCATTTTCATTTTCATTTTCACCCACAGGACTGATACAAATCTTAAAGACTCTTGTGTCATTTACTCATTTACGATTATTGCGAAGCACCATCATAGATTTCTAATTTCTTGCGACGATTTTAATTTCTCACTGATCCTGCAATTACATCAGTTGAAGCTCCAAATTCTGGAAAATGGAGATGATTGTAGCAGGGTCATTGATACTGGGTCTTCTATTAGCTCTTCTCTTTGTCATCCCACGATTTACCAAACCCAGTCAATCGAAAACTGAAGCCTTGAATAATAACCCCAAAAACATATCTAAGGAATCTAGAAGATATACTAAAGCTGAAGTTTCGTTGCATGACAAAAGGACTGATTGTTGGGTCATCATCAAAGATAAGGTATATGATGTAACTTCATATGTGGAGGAGCATCCAGGTGGTGATGCTATCTTAACGCATGCAGGGGATGATTCCACTGAAGGGTTCTTTGGGTATGCACCTTCCATATCCGTTGCATTTGTTGCTATTAGAGGAATACACTTCCCATTACTTCTCTGGAAAATGTTATTGTGGCCTTTTCAGAAAATGTTATCCGTTGCATTTGTTCCGGGGTTGAAACGATGGAAGGAGCGTCCTTGA

Coding sequence (CDS)

ATGGAGATGATTGTAGCAGGGTCATTGATACTGGGTCTTCTATTAGCTCTTCTCTTTGTCATCCCACGATTTACCAAACCCAGTCAATCGAAAACTGAAGCCTTGAATAATAACCCCAAAAACATATCTAAGGAATCTAGAAGATATACTAAAGCTGAAGTTTCGTTGCATGACAAAAGGACTGATTGTTGGGTCATCATCAAAGATAAGGTATATGATGTAACTTCATATGTGGAGGAGCATCCAGGTGGTGATGCTATCTTAACGCATGCAGGGGATGATTCCACTGAAGGGTTCTTTGGGTATGCACCTTCCATATCCGTTGCATTTGTTGCTATTAGAGGAATACACTTCCCATTACTTCTCTGGAAAATGTTATTGTGGCCTTTTCAGAAAATGTTATCCGTTGCATTTGTTCCGGGGTTGAAACGATGGAAGGAGCGTCCTTGA

Protein sequence

MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFGYAPSISVAFVAIRGIHFPLLLWKMLLWPFQKMLSVAFVPGLKRWKERP
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo10197.1Spo10197.1mRNA


Homology
[Close]
BLAST of Spo10197.1 vs. NCBI nr
Match: gi|902214286|gb|KNA17458.1| (hypothetical protein SOVF_079800 [Spinacia oleracea])

HSP 1 Score: 200.7 bits (509), Expect = 1.800e-48
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1 

		  

Query: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR 60
           MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR
Sbjct: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR 60

Query: 61  TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG
Sbjct: 61  TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 101
[Close]
BLAST of Spo10197.1 vs. NCBI nr
Match: gi|731353979|ref|XP_010688338.1| (PREDICTED: cytochrome B5-like protein [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 158.7 bits (400), Expect = 8.000e-36
Identity = 82/101 (81.19%), Postives = 88/101 (87.13%), Query Frame = 1 

		  

Query: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR 60
           ME+IVA ++ILG LL LLFV PRF KPSQS  E  N+N KNISK  RRYTKAEVSLH+KR
Sbjct: 1   MEIIVAATVILGFLLLLLFVTPRFIKPSQSN-EVSNSNTKNISKGPRRYTKAEVSLHNKR 60

Query: 61  TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           TDCWVIIK KVYDVTSYVEEHPGGDAILTHAGDDSTEGF+G
Sbjct: 61  TDCWVIIKAKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYG 100
[Close]
BLAST of Spo10197.1 vs. NCBI nr
Match: gi|747063400|ref|XP_011078248.1| (PREDICTED: cytochrome B5-like protein [Sesamum indicum])

HSP 1 Score: 131.7 bits (330), Expect = 1.000e-27
Identity = 70/101 (69.31%), Postives = 77/101 (76.24%), Query Frame = 1 

		  

Query: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR 60
           M+++V  SL LG LL +L VIPR  K    K    NN      K S+ YTKAEVSLH+KR
Sbjct: 1   MDLLVVTSL-LGFLLGILIVIPRLRKSDHGKIAQSNNVSP---KASKAYTKAEVSLHNKR 60

Query: 61  TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           TDCW+IIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG
Sbjct: 61  TDCWIIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 97
[Close]
BLAST of Spo10197.1 vs. NCBI nr
Match: gi|225442456|ref|XP_002283614.1| (PREDICTED: cytochrome B5-like protein [Vitis vinifera])

HSP 1 Score: 126.7 bits (317), Expect = 3.400e-26
Identity = 64/99 (64.65%), Postives = 77/99 (77.78%), Query Frame = 1 

		  

Query: 3   MIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKRTD 62
           +IV  +++L +LL  L VIP+  K   SKT   ++     SK S+ YTKAE+SLH+K+TD
Sbjct: 2   LIVVVAVLLSVLLGALLVIPQLKKTGNSKTAQKSST----SKVSKTYTKAEISLHNKKTD 61

Query: 63  CWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           CW+IIKDKVYDVTSYVEEHPGGDAIL HAGDDSTEGFFG
Sbjct: 62  CWIIIKDKVYDVTSYVEEHPGGDAILAHAGDDSTEGFFG 96
[Close]
BLAST of Spo10197.1 vs. NCBI nr
Match: gi|1009168774|ref|XP_015902843.1| (PREDICTED: cytochrome B5-like protein [Ziziphus jujuba])

HSP 1 Score: 125.9 bits (315), Expect = 5.700e-26
Identity = 64/99 (64.65%), Postives = 76/99 (76.77%), Query Frame = 1 

		  

Query: 3   MIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKRTD 62
           MI   +L+LGL+L    +IPR  K  ++K +  N   K   K S+ Y+KAEVSLH+KRTD
Sbjct: 2   MIAVVALVLGLVLGAFILIPRNLKSDRTKNDQSNAKDK---KASKIYSKAEVSLHNKRTD 61

Query: 63  CWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           CW+IIKDKVYDVTSYVEEHPGGDAIL HAGDDSTEGF+G
Sbjct: 62  CWIIIKDKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYG 97
[Close]
BLAST of Spo10197.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RDE5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_079800 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.300e-48
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1 

		  

Query: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR 60
           MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR
Sbjct: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR 60

Query: 61  TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG
Sbjct: 61  TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 101
[Close]
BLAST of Spo10197.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BTH0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g197520 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 5.600e-36
Identity = 82/101 (81.19%), Postives = 88/101 (87.13%), Query Frame = 1 

		  

Query: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR 60
           ME+IVA ++ILG LL LLFV PRF KPSQS  E  N+N KNISK  RRYTKAEVSLH+KR
Sbjct: 1   MEIIVAATVILGFLLLLLFVTPRFIKPSQSN-EVSNSNTKNISKGPRRYTKAEVSLHNKR 60

Query: 61  TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           TDCWVIIK KVYDVTSYVEEHPGGDAILTHAGDDSTEGF+G
Sbjct: 61  TDCWVIIKAKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYG 100
[Close]
BLAST of Spo10197.1 vs. UniProtKB/TrEMBL
Match: A0A022QPS6_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a016506mg PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.900e-26
Identity = 64/101 (63.37%), Postives = 81/101 (80.20%), Query Frame = 1 

		  

Query: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKR 60
           M+++VA +L+LGL+L LL VIP   K    K + + +  K  ++ S+ YTK+EVSLH+KR
Sbjct: 1   MDLLVA-TLLLGLVLGLLVVIPLLRKSDHGKAKGVQS--KISAEASKAYTKSEVSLHNKR 60

Query: 61  TDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           TDCW+IIK+KVYDVTSYVEEHPGGDAIL HAGDDST+GFFG
Sbjct: 61  TDCWIIIKEKVYDVTSYVEEHPGGDAILVHAGDDSTDGFFG 98
[Close]
BLAST of Spo10197.1 vs. UniProtKB/TrEMBL
Match: A0A151T2S1_CAJCA (Cytochrome b5 OS=Cajanus cajan GN=KK1_015860 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.200e-25
Identity = 66/102 (64.71%), Postives = 78/102 (76.47%), Query Frame = 1 

		  

Query: 1   MEMIVAGSLILGLLLALLFVIPRFTKPSQS-KTEALNNNPKNISKESRRYTKAEVSLHDK 60
           M ++VA +L+LGL LALL   PR  K   + K +A  NN     K S+ Y+K EVSLH+K
Sbjct: 1   MVVVVALALVLGLFLALLLFNPRHRKSGNNGKAQASVNN----DKASKSYSKTEVSLHNK 60

Query: 61  RTDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           RTDCW+IIK++VYDVTSYVEEHPGGDAIL HAGDDSTEGFFG
Sbjct: 61  RTDCWIIIKNRVYDVTSYVEEHPGGDAILAHAGDDSTEGFFG 98
[Close]
BLAST of Spo10197.1 vs. UniProtKB/TrEMBL
Match: A0A061GU94_THECC (Cytochrome B5-like protein, n5,ATCB5LP,CB5LP OS=Theobroma cacao GN=TCM_037790 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.600e-25
Identity = 63/101 (62.38%), Postives = 75/101 (74.26%), Query Frame = 1 

		  

Query: 8   SLILGLLLALLFVIPRFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDKRTDCWVII 67
           +LILG+LL    +IPR  K + +     N N    SK S+ Y+K EV+LH+KRTDCW+II
Sbjct: 7   ALILGVLLGAFILIPRHGKSAHTNQVQSNGNN---SKASKSYSKTEVALHNKRTDCWIII 66

Query: 68  KDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFGYAPSISV 109
           KDKVYDVTSYVEEHPGGDAIL HAGDDSTEGF+G   + SV
Sbjct: 67  KDKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATSV 104
[Close]
BLAST of Spo10197.1 vs. ExPASy Swiss-Prot
Match: CYP5F_ARATH (Cytochrome B5-like protein OS=Arabidopsis thaliana GN=CB5LP PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.000e-23
Identity = 56/102 (54.90%), Postives = 76/102 (74.51%), Query Frame = 1 

		  

Query: 3   MIVAGSLILGLLLALLFVIP---RFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDK 62
           MI    L+LG L++ LF+I    R T  +Q K  + +   +++ +  + Y+K+EV++H+K
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRP-KSYSKSEVAVHNK 60

Query: 63  RTDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           R DCW+IIKDKVYD+TSYVEEHPGGDAIL HAGDDST+GFFG
Sbjct: 61  RNDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFG 101
[Close]
BLAST of Spo10197.1 vs. ExPASy Swiss-Prot
Match: ACET6_CERPU (Bifunctional delta 6-fatty acyl acetylenase/desaturase OS=Ceratodon purpureus PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.400e-10
Identity = 29/78 (37.18%), Postives = 49/78 (62.82%), Query Frame = 1 

		  

Query: 28  SQSKTEALNNNPKNIS-KESRRYTKAEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGGDA 87
           +Q KT       +++  K+   Y+ A+V+ HD+  DCW+I+K+KVYD++ + ++HPGG  
Sbjct: 41  AQGKTAGQTLRQRSVQDKKPGTYSLADVASHDRPGDCWMIVKEKVYDISRFADDHPGGTV 100

Query: 88  ILTHAGDDSTEGFFGYAP 105
           I T+ G D T+ F  + P
Sbjct: 101 ISTYFGRDGTDVFATFHP 118
[Close]
BLAST of Spo10197.1 vs. ExPASy Swiss-Prot
Match: DES6_PHYPA (Acyl-lipid (9-3)-desaturase OS=Physcomitrella patens subsp. patens GN=DES6 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.400e-10
Identity = 24/51 (47.06%), Postives = 40/51 (78.43%), Query Frame = 1 

		  

Query: 52  AEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFGY 103
           +EV++H+K +DCW+++K+KVYDV+++ +EHPGG  I T+ G D T+ F  +
Sbjct: 108 SEVAVHNKPSDCWIVVKNKVYDVSNFADEHPGGSVISTYFGRDGTDVFSSF 158
[Close]
BLAST of Spo10197.1 vs. ExPASy Swiss-Prot
Match: CYB5A_ARATH (Cytochrome b5 isoform A OS=Arabidopsis thaliana GN=CB5-A PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.400e-10
Identity = 28/55 (50.91%), Postives = 41/55 (74.55%), Query Frame = 1 

		  

Query: 46  SRRYTKAEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGG-DAILTHAGDDSTEGF 100
           ++ Y+  E + H+K+ DCWV+I  KVYDV+SY++EHPGG D +L  AG D+T+ F
Sbjct: 5   TKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDF 59
[Close]
BLAST of Spo10197.1 vs. ExPASy Swiss-Prot
Match: D5FAD_PHYPA (Acyl-lipid (8-3)-desaturase OS=Physcomitrella patens subsp. patens GN=DES5 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.700e-10
Identity = 32/70 (45.71%), Postives = 43/70 (61.43%), Query Frame = 1 

		  

Query: 37  NNPKNISKES--RRYTKAEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDD 96
           +N K   +E   ++YT  +VS H+   DCW++I  KVYDVTS++  HPGG  I   AG D
Sbjct: 22  SNSKGPEQEQTLKKYTLEDVSRHNTPADCWLVIWGKVYDVTSWIPNHPGGSLIHVKAGQD 81

Query: 97  STEGFFGYAP 105
           ST+ F  Y P
Sbjct: 82  STQLFDSYHP 91
[Close]
BLAST of Spo10197.1 vs. TAIR (Arabidopsis)
Match: AT1G60660.1 (cytochrome B5-like protein)

HSP 1 Score: 110.2 bits (274), Expect = 1.100e-24
Identity = 56/102 (54.90%), Postives = 76/102 (74.51%), Query Frame = 1 

		  

Query: 3   MIVAGSLILGLLLALLFVIP---RFTKPSQSKTEALNNNPKNISKESRRYTKAEVSLHDK 62
           MI    L+LG L++ LF+I    R T  +Q K  + +   +++ +  + Y+K+EV++H+K
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRP-KSYSKSEVAVHNK 60

Query: 63  RTDCWVIIKDKVYDVTSYVEEHPGGDAILTHAGDDSTEGFFG 102
           R DCW+IIKDKVYD+TSYVEEHPGGDAIL HAGDDST+GFFG
Sbjct: 61  RNDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFG 101
[Close]
BLAST of Spo10197.1 vs. TAIR (Arabidopsis)
Match: AT1G26340.1 (cytochrome B5 isoform A)

HSP 1 Score: 65.9 bits (159), Expect = 2.500e-11
Identity = 28/55 (50.91%), Postives = 41/55 (74.55%), Query Frame = 1 

		  

Query: 46  SRRYTKAEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGG-DAILTHAGDDSTEGF 100
           ++ Y+  E + H+K+ DCWV+I  KVYDV+SY++EHPGG D +L  AG D+T+ F
Sbjct: 5   TKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDF 59
[Close]
BLAST of Spo10197.1 vs. TAIR (Arabidopsis)
Match: AT5G48810.1 (cytochrome B5 isoform D)

HSP 1 Score: 64.3 bits (155), Expect = 7.200e-11
Identity = 27/56 (48.21%), Postives = 39/56 (69.64%), Query Frame = 1 

		  

Query: 45  ESRRYTKAEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGGD-AILTHAGDDSTEGF 100
           + + +T +EVS H    DCW++I  KVYDVT ++++HPGGD  ILT  G D+T+ F
Sbjct: 4   DGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDF 59
[Close]
BLAST of Spo10197.1 vs. TAIR (Arabidopsis)
Match: AT2G32720.1 (cytochrome B5 isoform B)

HSP 1 Score: 63.2 bits (152), Expect = 1.600e-10
Identity = 26/56 (46.43%), Postives = 42/56 (75.00%), Query Frame = 1 

		  

Query: 45  ESRRYTKAEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGG-DAILTHAGDDSTEGF 100
           E++ +T +EVS H++  DCW++I  KVY+VT ++E+HPGG D +L+  G D+T+ F
Sbjct: 4   EAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDF 59
[Close]
BLAST of Spo10197.1 vs. TAIR (Arabidopsis)
Match: AT5G53560.1 (cytochrome B5 isoform E)

HSP 1 Score: 60.5 bits (145), Expect = 1.000e-9
Identity = 27/59 (45.76%), Postives = 40/59 (67.80%), Query Frame = 1 

		  

Query: 42  ISKESRRYTKAEVSLHDKRTDCWVIIKDKVYDVTSYVEEHPGGD-AILTHAGDDSTEGF 100
           +S + +  +  EVS H+K  DCW+II  KVYDVT ++++HPGGD  +L+  G D+T  F
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDF 59
The following BLAST results are available for this feature:
BLAST of Spo10197.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902214286|gb|KNA17458.1|1.8e-48100.hypothetical protein SOVF_0798... [more]
gi|731353979|ref|XP_010688338.1|8.0e-3681.1PREDICTED: cytochrome B5-like ... [more]
gi|747063400|ref|XP_011078248.1|1.0e-2769.3PREDICTED: cytochrome B5-like ... [more]
gi|225442456|ref|XP_002283614.1|3.4e-2664.6PREDICTED: cytochrome B5-like ... [more]
gi|1009168774|ref|XP_015902843.1|5.7e-2664.6PREDICTED: cytochrome B5-like ... [more]
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BLAST of Spo10197.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RDE5_SPIOL1.3e-48100.Uncharacterized protein OS=Spi... [more]
A0A0J8BTH0_BETVU5.6e-3681.1Uncharacterized protein OS=Bet... [more]
A0A022QPS6_ERYGU8.9e-2663.3Uncharacterized protein OS=Ery... [more]
A0A151T2S1_CAJCA1.2e-2564.7Cytochrome b5 OS=Cajanus cajan... [more]
A0A061GU94_THECC2.6e-2562.3Cytochrome B5-like protein, n5... [more]
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BLAST of Spo10197.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
CYP5F_ARATH2.0e-2354.9Cytochrome B5-like protein OS=... [more]
ACET6_CERPU4.4e-1037.1Bifunctional delta 6-fatty acy... [more]
DES6_PHYPA4.4e-1047.0Acyl-lipid (9-3)-desaturase OS... [more]
CYB5A_ARATH4.4e-1050.9Cytochrome b5 isoform A OS=Ara... [more]
D5FAD_PHYPA5.7e-1045.7Acyl-lipid (8-3)-desaturase OS... [more]
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BLAST of Spo10197.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT1G60660.11.1e-2454.9cytochrome B5-like protein[more]
AT1G26340.12.5e-1150.9cytochrome B5 isoform A[more]
AT5G48810.17.2e-1148.2cytochrome B5 isoform D[more]
AT2G32720.11.6e-1046.4cytochrome B5 isoform B[more]
AT5G53560.11.0e-945.7cytochrome B5 isoform E[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3D3.10.120.10coord: 45..105
score: 3.4
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 50..103
score: 3.7
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 49..112
score: 3.4
IPR001199Cytochrome b5-like heme/steroid binding domainPROFILEPS50255CYTOCHROME_B5_2coord: 46..117
score: 20
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 43..107
score: 2.22
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 77..84
scor
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..101
score: 3.9
NoneNo IPR availablePANTHERPTHR19359:SF10SUBFAMILY NOT NAMEDcoord: 1..101
score: 3.9

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0015994 chlorophyll metabolic process
biological_process GO:0016192 vesicle-mediated transport
biological_process GO:0006886 intracellular protein transport
biological_process GO:0009738 abscisic acid-activated signaling pathway
biological_process GO:0032984 protein-containing complex disassembly
biological_process GO:0035265 organ growth
biological_process GO:0042631 cellular response to water deprivation
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0042542 response to hydrogen peroxide
biological_process GO:0009407 toxin catabolic process
biological_process GO:0010207 photosystem II assembly
biological_process GO:0016556 mRNA modification
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005768 endosome
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005802 trans-Golgi network
cellular_component GO:0010007 magnesium chelatase complex
cellular_component GO:0005622 intracellular
cellular_component GO:0005829 cytosol
cellular_component GO:0016020 membrane
cellular_component GO:0009523 photosystem II
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009535 chloroplast thylakoid membrane
molecular_function GO:0005484 SNAP receptor activity
molecular_function GO:0020037 heme binding
molecular_function GO:0016851 magnesium chelatase activity
molecular_function GO:0046422 violaxanthin de-epoxidase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0005525 GTP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004602 glutathione peroxidase activity
RNA-Seq Expression