Spo09453 (gene) | SpinachBase

Overview


Name	Spo09453
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Methylenetetrahydrofolate reductase (1.5.1.20)
Location	chr3 : 17574637 .. 17580209 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATGGTAGTAGGTGGCAACTCTTTTTTAAAAAAAATCAAAATAAGATTTGCTTTCAGCCTCATGCTCTCACTCTCACCCACCCTCTCTCCTCTCCTATATTAACCCCCCTATTCTCCTCTCCACTCCTTGCCACACTCCTCTCTTCCTCCCTCCATTGCCAGATTCAATCTTCTTCTCCAGGAAAAATATGAAGGTAATCGACAAAATCAAAGCGTCGGAATCCTCAAACCAAATCCTCTTCTCTTTTGAATACTTCCCCCCTAAAACCGACGATGGAGTCGAAAATCTTTTCGATAGAATGGAACGAATGGTTTCACACTCCCCTTCCTTTTGCGACATTACTTGGGGTGCTGGTGGATCTACTGCCGATCTTACCCTAGAAATCGCAAATCGTATGCAGAACACTGTTTGTGTTGAGACTATGATGCACTTGACTTGTACTAATATGCCTGTTGAGAAGATCGATCATGCTCTTGATACTATCAAGGCAAATGGGTTGCAAAACGTTCTTGCTCTTCGTGGGGATCCACCCCATGGACAGGACAAGTTTGTTCAGGTTGAAGGCGGATTCGCTTGCGCGCTTGATCTTGTGAGTTTTACTCTGTATTTGTTGTTATGATTTGCGAACTGTTCAATTGCGATGTTGTTGTTGGTTCCATGTTAGATCTAATTTTCGGATATTCAATTTTTTGATTTCAGGTTAATCATATGAGGAGCAAGTATGGGGATTACTTTGGGATTACTGTTGCTGGTTATCCAGGTGAGCTGGGATAATACTCGTATAATTTTGCATAATTTTGTACTCCGTATTTAGTAATAAAATTGTGGATTGGCAAGTGTTTTTTATAATTGCGATTGTAAAAGTATGGTAAATTAGCAGTATGAATTAGTCATTAGCTTTGGTTTCTGCTTTAATTGGTTGCTTTAGCGGACTATAATTGATAATTTAAGTGGTAAATAGCATTATGAATTAGTCATTAGCTAAGATTTTTGCTTTAAATGGTTTCTTAAGCTGAATATAATTGATAATTTAAGTGTTAATTGCGTGTTTAGTCATCAAAGTGGTTCTTTTATTAGCATATATTGAGGATCTGAATTTAGTCTGGTAATGTAATATTTACATTTTACTCCGTAGAAAATAATGTGCTTTATCACACTTTATCAAAATGTAGTTTTTCTAATTAATTCTACTATTGGTGGGTTTAATCTGTTTTTACCAAAGACTATCAAGTTCTTTACAGTAATGTTTATTAAGTATCGAACCCATGGAAAGTTGTGTCGTTCACTTACAGGAGAAAACTTTGGGTTGTAATAGAAGTTTCAAAGTTTTTCCCATCTATTACAGATGAAATGCACATGATTTTGTTTGGGTAAAAACTAATCGAGTCATTTCCTGGTGGCTTACGGTTATTCTGTTTCATAATGTTTTGATGTAGCAAGTTTCATATTTCTAGTACTGAGTTAATGAAGTGGTGGTTTCTCACTTTGATTAGTATCTGATATGCTGTCGTATTTTGAAATAATTTAGAGGCACATCCTGATGCTATTGAAGAGAATGGTTTGGCTACTCCAGAATCATATGCTAGTGATCTGGCTTACTTAAAGAAAAAGGTAATATATCTTTTTCTCTGAAGTAATGCCATTACATTTGTGTATGTGTATGATCGCAATGCATTAGGTATATGATGACTTGATCTTGCGCTGCATTTATGCTTGCGCTTACACTGTTTCTTTTGCTAATGGCATAGGTTGATGCTGGTGCTGATCTTATCGTCACTCAGCTGTTTTATGATACCGATGTTTTTCTGAAGTTTGTTAATGATTGCCGCCAAATTGGAATTAATTGTCCAATTGTTCCTGGCATCATGCCCATAAATAACTACAAAGGATTTATACGTATGACTGGTTTCTGTAAAACAAAGGTAAGATCTTTTTACTTTATAGATACTACTATATCTAATTGACATTTAAATGTGATTCTGGGATTTGAGTTTTCTATTATTCTGACTTTTGCTTCATGCCCTGAACTAACTTGCATGTTTTTGGCTGGAAAAATGTTGTAGATTCCTCAAGAGGTCACTGATGCATTGGAGCCAATTAAGGACAATGACGAAGCAGTGAAAGCCTATGGTATTCATCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTAGTGGAATCAAGCAGTTGCATCTTTATACCTTGAACATGGACAAATCTGCACTAGGAATACTCCTGGTAGCTTCTCACATCCTCTTTCATATAATGACCTTCTTGCTTGTAGTATGTTGATTTATATTTTATACAACTGCCGCAGAATCTTGGATTGATTGAAGAGTCCAAAATTCAAAGGCCATTACCTTGGAAACGGCCAGCAAATGTTAACCGTACTAAGGAAGATGTTAGACCCATATTTTGGTATGTGCAATTCTTAATTTAATGAGTTTTAAATGCACAATCCTACTAATCTATGTCTGAAGATATTAATTTGTTTGGCATTCTCGATGATGGTTACAGGGCTAACCGTCCAAAAAGCTACATCTCTCGAACAATGGGATGGGATCAATACCCACAAGGGCGATGGGGTGATGCTCGTAATGCTTCTTATGGAGCATTAAGTGACCATCAGGTAACTTGAAGCCTTTTTCTGAAACTGGTTTATTGAAAATATCTTTTGTAATGTTGCTGAATTTAGTATACATGAGCAAACTTGAATATGTACTGTTAAGTCCGGTTACTTGAAAAATTAGCCCATGTACTATAAAGCTGAAGAGTACCCTCCTCTGTTGACACCCATATCAGTTTCTGGTTACAAAAGTGAAAAAGTAGGGCACTGACTACTCTTGAATTTTCATAACTAATCTCGAAACCATGTACTATGGTTTGTTGAAGGTGGAGTGTCGACAAAATTGGAAAAAGAGCCTTGAACTATGGCTAGTCATATACTCCCTAACATAGGTTCTGCAGGGCTTGAATGTATCAAGAAGAAATAAGAAACCTTCACTTCATTTTGCCAGACTGTCTATCTCATGCCCAGAGAATCAGAGATATGTTAGCGTATTCTCATGTACTATGTGCTCATCTTTGCTTTGACTTGTGCTCTCCCCTTTATCCCCTGCCTTTTGCTTGTCTTTTGCTTGCTCTGATCATTAATCTTATTCTTAATTCTATTGAGTGTAATTACCTTTGCCAGTCACATCTGTATTCTTGTTCTGTAAGTTTTGGATGAAGCTTCCGTTTGGCTTGAATGATGCACATTTGCCTAGCTTTTGTAGAATTTCTGTTTCAGTTGAGACTGAGATATTTCTCTGAAGTCTGGACATGTTATTTTATGTTTGTTACTCAAAAACTAATGGTAACATAATGCTTCTCTGTCTCGTGACAGTTTATGAGACCTCGTGCACGTGACAAGAAGCTGCAAGAAGAGTGGGCTGTCCCGCTGCAAAACATTGAAGATATATACGAGGTAGACTTCTATTTCTGCCTACATGCTGCAATCATTGGTAGAATGTTGATACCAGTTTTGTGGTGCCTGATGCTTTCTTTGTTATTTCAGATGTTCAAGAACTTTTGCCTTGGTAAGCTGAGAAGCAATCCTTGGTCTGAACTAGATGGGCTTCAGCCAGAAACTAAAATCATAGATGAACAACTTGGCAACATTAATAAGAAGGGTTTCCTCACCATCAACAGCCAACCGGCAGTTAATGGAGAAAGATCAGACTCCCCTACTGTTGGTAAGTCCTAAAATATCCACAGATATTATTTTAGTGTTTTCCCTTGTGCAGTTATATACCTTATTCTTTGTTCAACTCCAAGATGTATTTTGTTAAATGGTGATATTTTTAATTTAGTTGTTTTAATTCGAAACGAATTTAATCTGCGATATTGTGTTGATGGAAAGTTGGTATCAACTAAAAAGAATCTAAAAGCAATTTTACGTCGTGTAGTAGTGGAGCCTTGTGTGTGTGTCCCCTGATTTGATATCTTAGGATGCATAATTTTTTCCAACCGTTGGTGTGTACAAGTTTTCTATATAGAGTTATGTACTGCATTTCCAAGAAGAGGTACTTGGTCTATCCTTTTACTGGTTGTCTGCACATGTCTACTGGTATTTCTATTTTATGTTGTTGTCTGTGTTTCTGATCAGGACTTCAGGTCAGAATTAGTTATATTATCTGCTATTTTTCAAGTGCTACATTCCCTTGATGTCTTGTTGCAGTTTTTGAATGATGTTTTCCAATAGCACATAATATCATTGTTCCTAGGCTAAAAAGACAAGTGTGTTGGTGACTGTTGCTTTTTACACTACTTCGTTGATTAGTTAAAAGGAATAACGAATGAGGATCACATCTCTTGTCGGAGTTGATTTTAGTCGGGCTTCTATTGCAAATTGGCTAAAAATTTATGTTCTACCTTTTTTCTGTAATATATGCTTTTTTCAGGAACCTCAGATTGAATTTTCATCATTACTAAGATATAATAAACTTTAACCAAATGCTAATTTTCTTCAGGATGGGGTGGGCCTGGTGGATATGTATACCAGAAGGCATATGTAGAATTCTTTTGCTCGAAGGAGAAGTTGAACGCGCTTGTTGAAAAATGCAAAGCTCTCAAACACATAACCTACATGGCAGTAAACAAAGGAGGAGATTGGATCTCAAATGTAGCTCAAACTGGTGTGAACGCCGTGACATGGGGAGTGCTCCCTGCAAAGGAGGTTATACAACCAACTGTCGTGGATCCTGCCAGCTTTATGATCTGGAAAGACGAGGCATTTGAGATCTGGTCAAGATCGTGGGCTAAATTGTACCCAGAGGGTGACTCGTCCAAGACACTGCTCGAACAGGTTTGTGCCTTTGACAACTTTCTTTCTCCAATTGTTGAGTTAGCTTGATTGCTTATTATCATCTTCTATACTATAGTGTTTGCACTTTTGTCTGATGACATATATGTTACTTATACAGGTGCAAAACAACTACTACTTGGTCAGCTTGGTTGACAATGATTACATCAATGGTGATCTGTTTTCTGTTTTCACCGATCTTTGAGATGATAATATGTTACTGCTACGTTATTTGCTCTATTCGTTTAGTGATGTATTGTATACCATTGGCCAAAAACCTTGTCCACTGTTCCCTCTATTATTATTAGATTTAGTTGAGTTTGTAATGCCATAACCTTGTGGGATTTTGCGATCCTCCAAGCCTCCCAATGCAAATTGCATGGTGAGTTCTTCTTCTGCTTAATGTTGTATTTTATTCGAGTTATATTATATAAGCATCTGGTTCTGGCTTTTTGGCGATATTTGTTTGTCATTTATTTATTTATCAGGGTAAATTTTGTTGCGAGTAATTTGAATGCACAACTTATTGTACCTTATGCTTTGGCGTCTTTCCATTAAAATAATATAAAATCCTAACAGTGAAGGAGGCCTTAGTTAATCACAACTAAGATACTACGCTGCATCTGTTGTACTCTGTGTGAGCTAATACAGGCTATACAGCCCAAGCAGATTATATGCACTGACTAATTCTGGAAAAAAAAAACTCTAATTTCAATGCGGAACATCTAC

mRNA sequence

GAATGGTAGTAGGTGGCAACTCTTTTTTAAAAAAAATCAAAATAAGATTTGCTTTCAGCCTCATGCTCTCACTCTCACCCACCCTCTCTCCTCTCCTATATTAACCCCCCTATTCTCCTCTCCACTCCTTGCCACACTCCTCTCTTCCTCCCTCCATTGCCAGATTCAATCTTCTTCTCCAGGAAAAATATGAAGGTAATCGACAAAATCAAAGCGTCGGAATCCTCAAACCAAATCCTCTTCTCTTTTGAATACTTCCCCCCTAAAACCGACGATGGAGTCGAAAATCTTTTCGATAGAATGGAACGAATGGTTTCACACTCCCCTTCCTTTTGCGACATTACTTGGGGTGCTGGTGGATCTACTGCCGATCTTACCCTAGAAATCGCAAATCGTATGCAGAACACTGTTTGTGTTGAGACTATGATGCACTTGACTTGTACTAATATGCCTGTTGAGAAGATCGATCATGCTCTTGATACTATCAAGGCAAATGGGTTGCAAAACGTTCTTGCTCTTCGTGGGGATCCACCCCATGGACAGGACAAGTTTGTTCAGGTTGAAGGCGGATTCGCTTGCGCGCTTGATCTTGTTAATCATATGAGGAGCAAGTATGGGGATTACTTTGGGATTACTGTTGCTGGTTATCCAGAGGCACATCCTGATGCTATTGAAGAGAATGGTTTGGCTACTCCAGAATCATATGCTAGTGATCTGGCTTACTTAAAGAAAAAGGTTGATGCTGGTGCTGATCTTATCGTCACTCAGCTGTTTTATGATACCGATGTTTTTCTGAAGTTTGTTAATGATTGCCGCCAAATTGGAATTAATTGTCCAATTGTTCCTGGCATCATGCCCATAAATAACTACAAAGGATTTATACGTATGACTGGTTTCTGTAAAACAAAGATTCCTCAAGAGGTCACTGATGCATTGGAGCCAATTAAGGACAATGACGAAGCAGTGAAAGCCTATGGTATTCATCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTAGTGGAATCAAGCAGTTGCATCTTTATACCTTGAACATGGACAAATCTGCACTAGGAATACTCCTGAATCTTGGATTGATTGAAGAGTCCAAAATTCAAAGGCCATTACCTTGGAAACGGCCAGCAAATGTTAACCGTACTAAGGAAGATGTTAGACCCATATTTTGGGCTAACCGTCCAAAAAGCTACATCTCTCGAACAATGGGATGGGATCAATACCCACAAGGGCGATGGGGTGATGCTCGTAATGCTTCTTATGGAGCATTAAGTGACCATCAGTTTATGAGACCTCGTGCACGTGACAAGAAGCTGCAAGAAGAGTGGGCTGTCCCGCTGCAAAACATTGAAGATATATACGAGATGTTCAAGAACTTTTGCCTTGGTAAGCTGAGAAGCAATCCTTGGTCTGAACTAGATGGGCTTCAGCCAGAAACTAAAATCATAGATGAACAACTTGGCAACATTAATAAGAAGGGTTTCCTCACCATCAACAGCCAACCGGCAGTTAATGGAGAAAGATCAGACTCCCCTACTGTTGGATGGGGTGGGCCTGGTGGATATGTATACCAGAAGGCATATGTAGAATTCTTTTGCTCGAAGGAGAAGTTGAACGCGCTTGTTGAAAAATGCAAAGCTCTCAAACACATAACCTACATGGCAGTAAACAAAGGAGGAGATTGGATCTCAAATGTAGCTCAAACTGGTGTGAACGCCGTGACATGGGGAGTGCTCCCTGCAAAGGAGGTTATACAACCAACTGTCGTGGATCCTGCCAGCTTTATGATCTGGAAAGACGAGGCATTTGAGATCTGGTCAAGATCGTGGGCTAAATTGTACCCAGAGGGTGACTCGTCCAAGACACTGCTCGAACAGGTGCAAAACAACTACTACTTGGTCAGCTTGGTTGACAATGATTACATCAATGGTGATCTGTTTTCTGTTTTCACCGATCTTTGAGATGATAATATGTTACTGCTACGTTATTTGCTCTATTCGTTTAGTGATGTATTGTATACCATTGGCCAAAAACCTTGTCCACTGTTCCCTCTATTATTATTAGATTTAGTTGAGTTTGTAATGCCATAACCTTGTGGGATTTTGCGATCCTCCAAGCCTCCCAATGCAAATTGCATGGTGAGTTCTTCTTCTGCTTAATGTTGTATTTTATTCGAGTTATATTATATAAGCATCTGGTTCTGGCTTTTTGGCGATATTTGTTTGTCATTTATTTATTTATCAGGGTAAATTTTGTTGCGAGTAATTTGAATGCACAACTTATTGTACCTTATGCTTTGGCGTCTTTCCATTAAAATAATATAAAATCCTAACAGTGAAGGAGGCCTTAGTTAATCACAACTAAGATACTACGCTGCATCTGTTGTACTCTGTGTGAGCTAATACAGGCTATACAGCCCAAGCAGATTATATGCACTGACTAATTCTGGAAAAAAAAAACTCTAATTTCAATGCGGAACATCTAC

Coding sequence (CDS)

ATGAAGGTAATCGACAAAATCAAAGCGTCGGAATCCTCAAACCAAATCCTCTTCTCTTTTGAATACTTCCCCCCTAAAACCGACGATGGAGTCGAAAATCTTTTCGATAGAATGGAACGAATGGTTTCACACTCCCCTTCCTTTTGCGACATTACTTGGGGTGCTGGTGGATCTACTGCCGATCTTACCCTAGAAATCGCAAATCGTATGCAGAACACTGTTTGTGTTGAGACTATGATGCACTTGACTTGTACTAATATGCCTGTTGAGAAGATCGATCATGCTCTTGATACTATCAAGGCAAATGGGTTGCAAAACGTTCTTGCTCTTCGTGGGGATCCACCCCATGGACAGGACAAGTTTGTTCAGGTTGAAGGCGGATTCGCTTGCGCGCTTGATCTTGTTAATCATATGAGGAGCAAGTATGGGGATTACTTTGGGATTACTGTTGCTGGTTATCCAGAGGCACATCCTGATGCTATTGAAGAGAATGGTTTGGCTACTCCAGAATCATATGCTAGTGATCTGGCTTACTTAAAGAAAAAGGTTGATGCTGGTGCTGATCTTATCGTCACTCAGCTGTTTTATGATACCGATGTTTTTCTGAAGTTTGTTAATGATTGCCGCCAAATTGGAATTAATTGTCCAATTGTTCCTGGCATCATGCCCATAAATAACTACAAAGGATTTATACGTATGACTGGTTTCTGTAAAACAAAGATTCCTCAAGAGGTCACTGATGCATTGGAGCCAATTAAGGACAATGACGAAGCAGTGAAAGCCTATGGTATTCATCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTAGTGGAATCAAGCAGTTGCATCTTTATACCTTGAACATGGACAAATCTGCACTAGGAATACTCCTGAATCTTGGATTGATTGAAGAGTCCAAAATTCAAAGGCCATTACCTTGGAAACGGCCAGCAAATGTTAACCGTACTAAGGAAGATGTTAGACCCATATTTTGGGCTAACCGTCCAAAAAGCTACATCTCTCGAACAATGGGATGGGATCAATACCCACAAGGGCGATGGGGTGATGCTCGTAATGCTTCTTATGGAGCATTAAGTGACCATCAGTTTATGAGACCTCGTGCACGTGACAAGAAGCTGCAAGAAGAGTGGGCTGTCCCGCTGCAAAACATTGAAGATATATACGAGATGTTCAAGAACTTTTGCCTTGGTAAGCTGAGAAGCAATCCTTGGTCTGAACTAGATGGGCTTCAGCCAGAAACTAAAATCATAGATGAACAACTTGGCAACATTAATAAGAAGGGTTTCCTCACCATCAACAGCCAACCGGCAGTTAATGGAGAAAGATCAGACTCCCCTACTGTTGGATGGGGTGGGCCTGGTGGATATGTATACCAGAAGGCATATGTAGAATTCTTTTGCTCGAAGGAGAAGTTGAACGCGCTTGTTGAAAAATGCAAAGCTCTCAAACACATAACCTACATGGCAGTAAACAAAGGAGGAGATTGGATCTCAAATGTAGCTCAAACTGGTGTGAACGCCGTGACATGGGGAGTGCTCCCTGCAAAGGAGGTTATACAACCAACTGTCGTGGATCCTGCCAGCTTTATGATCTGGAAAGACGAGGCATTTGAGATCTGGTCAAGATCGTGGGCTAAATTGTACCCAGAGGGTGACTCGTCCAAGACACTGCTCGAACAGGTGCAAAACAACTACTACTTGGTCAGCTTGGTTGACAATGATTACATCAATGGTGATCTGTTTTCTGTTTTCACCGATCTTTGA

Protein sequence

MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTADLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDKFVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLKKKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTKIPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNLGLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNASYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPETKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLNALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWKDEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo09453.1	Spo09453.1	mRNA

Homology

[Close]

BLAST of Spo09453.1 vs. NCBI nr
Match: gi|902194486|gb|KNA12569.1| (hypothetical protein SOVF_124730 [Spinacia oleracea])

HSP 1 Score: 1234.9 bits (3194), Expect = 0.000e+0
Identity = 593/594 (99.83%), Postives = 593/594 (99.83%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA
Sbjct: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK
Sbjct: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KKVDA ADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK
Sbjct: 181 KKVDADADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL
Sbjct: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA
Sbjct: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE
Sbjct: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN
Sbjct: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK
Sbjct: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL
Sbjct: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 594

[Close]

BLAST of Spo09453.1 vs. NCBI nr
Match: gi|731323657|ref|XP_010672546.1| (PREDICTED: methylenetetrahydrofolate reductase 1-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 1144.4 bits (2959), Expect = 0.000e+0
Identity = 544/594 (91.58%), Postives = 571/594 (96.13%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVI+KI+A+E+ NQILFSFEYFPPKTDDGVENLF+RMERMVSHSPSFCDITWGAGGSTA
Sbjct: 1   MKVIEKIRAAEAQNQILFSFEYFPPKTDDGVENLFERMERMVSHSPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEIANRMQN VCVETMMHLTCTNMPVEKIDHAL TIK+NGLQNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIANRMQNIVCVETMMHLTCTNMPVEKIDHALTTIKSNGLQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLVNHMRSKYGDYFGIT+AGYPEAHPDAI  NGLAT E+YASDLAYLK
Sbjct: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITIAGYPEAHPDAIGANGLATEEAYASDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           +KVDAGADLIVTQLFYDTD FLKFVNDCRQIGI CPIVPGIMPINNYKGF RMTGFCKTK
Sbjct: 181 RKVDAGADLIVTQLFYDTDNFLKFVNDCRQIGITCPIVPGIMPINNYKGFQRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IPQE+TDALEPIKDN+EAVKAYGIHLGTEMCKKILASGIKQLHLYTLNM+KSALGILLNL
Sbjct: 241 IPQEITDALEPIKDNEEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMEKSALGILLNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKIQRPLPWKRPANVNR KEDVRPIFWANRPKSYI+RTMGWDQYP GRWGDARNA
Sbjct: 301 GLIEESKIQRPLPWKRPANVNRVKEDVRPIFWANRPKSYITRTMGWDQYPHGRWGDARNA 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGALSDHQFMRPRARDKKLQEEWAVPLQ+ E I+EMFKNFCLGKL+SNPWSELDGLQPE
Sbjct: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQSAEAIHEMFKNFCLGKLKSNPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKIIDE+LG+IN KGFLTINSQPAVNGE+SDSPTVGWGGPGGYVYQKAYVEFFC+KEKLN
Sbjct: 421 TKIIDEKLGDINMKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCAKEKLN 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           ALVEKCK+L  ITYMAVNKGGD ISNVAQT VNAVTWG  P+KE+IQPTVVD ASFMIWK
Sbjct: 481 ALVEKCKSLTSITYMAVNKGGDLISNVAQTDVNAVTWGAFPSKEIIQPTVVDSASFMIWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWSRSWA+LYPEGDSSK LLE+VQN+YYLVSLVDNDYI+GDLFSVFT L
Sbjct: 541 DEAFEIWSRSWAQLYPEGDSSKKLLEEVQNSYYLVSLVDNDYIHGDLFSVFTGL 594

[Close]

BLAST of Spo09453.1 vs. NCBI nr
Match: gi|470101473|ref|XP_004287197.1| (PREDICTED: methylenetetrahydrofolate reductase 2-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 1073.9 bits (2776), Expect = 9.200e-311
Identity = 497/594 (83.67%), Postives = 552/594 (92.93%), Query Frame = 1


		  
Query: 1   MKVIDKI-KASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGST 60
           MKVIDKI +AS    +++FSFE+FPPKT+DGVENL +RM+RMV+H+P+FCDITWGAGGST
Sbjct: 1   MKVIDKILEASADEKKVVFSFEFFPPKTEDGVENLLERMDRMVAHNPAFCDITWGAGGST 60

Query: 61  ADLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQD 120
           ADLTL+IAN+MQN +CVE+MMHLTCTNMPV+KIDHAL TIK+NGLQNVLALRGDPPHGQD
Sbjct: 61  ADLTLDIANKMQNVICVESMMHLTCTNMPVDKIDHALQTIKSNGLQNVLALRGDPPHGQD 120

Query: 121 KFVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYL 180
           KFVQ++GGFACALDLV H+R+ YGDYFGITVAGYPEAHPDAIE NGLATPESY SDLAYL
Sbjct: 121 KFVQIQGGFACALDLVTHIRATYGDYFGITVAGYPEAHPDAIEANGLATPESYQSDLAYL 180

Query: 181 KKKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKT 240
           K+KVDAGADLIVTQLFYDTDVFLKFVNDCRQIGI CPIVPGIMPINNYKGFIRMTGFCKT
Sbjct: 181 KRKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCKT 240

Query: 241 KIPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLN 300
           +IP EVT ALEPIKDN+EAV+AYGIHLGTEMCKKILA GI+ LHLYTLNM+KSAL IL+N
Sbjct: 241 RIPAEVTAALEPIKDNEEAVRAYGIHLGTEMCKKILAHGIRTLHLYTLNMEKSALAILMN 300

Query: 301 LGLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARN 360
           LGLIEE+KI RPLPW+RPANV R KEDVRPIFWANRPKSYISRT+GWD YP GRWGD+RN
Sbjct: 301 LGLIEEAKISRPLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDLYPHGRWGDSRN 360

Query: 361 ASYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQP 420
            SYGAL+D+QFMRPRARDKKL EEW VPL+N+EDI+E F+ FCLGKLRS+PWSELDGLQP
Sbjct: 361 PSYGALTDYQFMRPRARDKKLVEEWVVPLKNVEDIHEKFEKFCLGKLRSSPWSELDGLQP 420

Query: 421 ETKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKL 480
           ETKII+E+LG IN KGFLTINSQPA+NGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKL
Sbjct: 421 ETKIINEKLGKINTKGFLTINSQPAINGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKL 480

Query: 481 NALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIW 540
             LV+KCKAL  +TYMAVN+ G WISN+ QT VNAVTWGV PAKE+IQPTVVDPASFM+W
Sbjct: 481 GTLVDKCKALPSLTYMAVNREGSWISNIGQTDVNAVTWGVFPAKEIIQPTVVDPASFMVW 540

Query: 541 KDEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTD 594
           KDEAFEIWSR W++LYPEGDSS+ LLE+VQN+Y+LVSLVDNDYINGDLF+VF D
Sbjct: 541 KDEAFEIWSRGWSRLYPEGDSSRKLLEEVQNSYFLVSLVDNDYINGDLFTVFAD 594

[Close]

BLAST of Spo09453.1 vs. NCBI nr
Match: gi|565373349|ref|XP_006353237.1| (PREDICTED: methylenetetrahydrofolate reductase 2-like [Solanum tuberosum])

HSP 1 Score: 1070.1 bits (2766), Expect = 1.400e-309
Identity = 496/594 (83.50%), Postives = 551/594 (92.76%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVI+KI+ +  +++++FSFE+FPPKTDDGVENLF+RMERMV+HSPSFCDITWGAGGSTA
Sbjct: 1   MKVIEKIQEAAKTDKVIFSFEFFPPKTDDGVENLFERMERMVAHSPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTL+I NRMQN VCVE+MMHLTCTNMPVEKIDHALDTIKANG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLDIVNRMQNMVCVESMMHLTCTNMPVEKIDHALDTIKANGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLV HMR+KYGDYFGITVAGYPEAHPD I  NG+AT E Y +DLAYLK
Sbjct: 121 FVQVEGGFACALDLVKHMRAKYGDYFGITVAGYPEAHPDVIPANGIATKEIYENDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KKVDAGAD+IVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGF+RMTGFCKTK
Sbjct: 181 KKVDAGADVIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFLRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP+E+  ALEPIKDN+EAVKAYGIHLGTEMCKKILA+GIK LHLYTLNM+KSAL IL+NL
Sbjct: 241 IPEEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALAILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKI RPLPW+RPANV R KEDVRPIFWANRPKSYISRT GWD+YP GRW +A+N 
Sbjct: 301 GLIEESKISRPLPWRRPANVFRVKEDVRPIFWANRPKSYISRTTGWDEYPHGRWSNAQNP 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGAL+D+QFMR R+RDKKLQEEWAV L ++EDIYE FK++CLGKLRS PWSELDGLQPE
Sbjct: 361 SYGALTDYQFMRARSRDKKLQEEWAVALNSVEDIYEKFKDYCLGKLRSCPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKII+E+LG +N KGFLTINSQPAVN E+SDSP+VGWGGPGGYVYQKAY+E FCS+EKLN
Sbjct: 421 TKIINEKLGQVNTKGFLTINSQPAVNAEKSDSPSVGWGGPGGYVYQKAYLELFCSQEKLN 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           ALVEKCK+  ++TYMAVNK G+WISNV QT VNAVTWGV PAKE+IQPTVVDPASFM+WK
Sbjct: 481 ALVEKCKSFPYLTYMAVNKEGNWISNVNQTDVNAVTWGVFPAKEIIQPTVVDPASFMVWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWSR WA+LYPE D S+ LLEQVQN+Y+LVSLVDNDYINGDLFS+F D+
Sbjct: 541 DEAFEIWSRGWAQLYPETDPSRKLLEQVQNSYFLVSLVDNDYINGDLFSIFKDI 594

[Close]

BLAST of Spo09453.1 vs. NCBI nr
Match: gi|970062966|ref|XP_015058397.1| (PREDICTED: methylenetetrahydrofolate reductase 2-like [Solanum pennellii])

HSP 1 Score: 1068.9 bits (2763), Expect = 3.100e-309
Identity = 494/594 (83.16%), Postives = 549/594 (92.42%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVI+KI+ +  +++++FSFE+FPPKTDDGVENLF+RMERMV+H PSFCDITWGAGGSTA
Sbjct: 1   MKVIEKIQEAAKTDKVIFSFEFFPPKTDDGVENLFERMERMVAHGPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEI NRMQN VCVE+MMHLTCTNMPVEKIDHALDTIKANG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIVNRMQNMVCVESMMHLTCTNMPVEKIDHALDTIKANGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLV HMR+KYGDYFGIT+AGYPEAHPD I  NG+AT E Y +DLAYLK
Sbjct: 121 FVQVEGGFACALDLVKHMRAKYGDYFGITIAGYPEAHPDVIPANGIATKEIYENDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KKVDAGAD+IVTQLFYDTD+FLKFVNDCRQIGINCPIVPGIMPINNYKGF+RMTGFCKTK
Sbjct: 181 KKVDAGADVIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPINNYKGFLRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP E+  ALEPIKDN+EAVKAYGIHLGTEMCKKILA+GIK LHLYTLNM+KSAL IL+NL
Sbjct: 241 IPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALAILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKI RPLPW+RPANV R KEDVRPIFWANRPKSYISRT GWD+YP GRW +A+N 
Sbjct: 301 GLIEESKISRPLPWRRPANVFRVKEDVRPIFWANRPKSYISRTTGWDEYPHGRWSNAQNP 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGAL+D+QFMR R+RDKKLQEEWA  L ++EDIYE FK++CLGKLRS PWSELDGLQPE
Sbjct: 361 SYGALTDYQFMRARSRDKKLQEEWAAALNSVEDIYEKFKDYCLGKLRSCPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKII+E+LG +N KGFLTINSQPAVN E+SDSP+VGWGGPGGYVYQKAY+EFFCS+EKLN
Sbjct: 421 TKIINEKLGQVNTKGFLTINSQPAVNAEKSDSPSVGWGGPGGYVYQKAYLEFFCSQEKLN 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           ALVEKCK+  ++TYMAVNK G+WISNV QT +NAVTWGV PAKE+IQPTVVDPASFMIWK
Sbjct: 481 ALVEKCKSFPYLTYMAVNKEGNWISNVNQTDINAVTWGVFPAKEIIQPTVVDPASFMIWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWSR WA+LYPE D S+ LLEQVQN+Y+LVSLVDNDYINGDLFS+F D+
Sbjct: 541 DEAFEIWSRGWAQLYPETDPSRKLLEQVQNSYFLVSLVDNDYINGDLFSIFKDI 594

[Close]

BLAST of Spo09453.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QZ74_SPIOL (Methylenetetrahydrofolate reductase OS=Spinacia oleracea GN=SOVF_124730 PE=3 SV=1)

HSP 1 Score: 1234.9 bits (3194), Expect = 0.000e+0
Identity = 593/594 (99.83%), Postives = 593/594 (99.83%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA
Sbjct: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK
Sbjct: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KKVDA ADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK
Sbjct: 181 KKVDADADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL
Sbjct: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA
Sbjct: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE
Sbjct: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN
Sbjct: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK
Sbjct: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL
Sbjct: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 594

[Close]

BLAST of Spo09453.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CU87_BETVU (Methylenetetrahydrofolate reductase OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g059580 PE=3 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.000e+0
Identity = 544/594 (91.58%), Postives = 571/594 (96.13%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVI+KI+A+E+ NQILFSFEYFPPKTDDGVENLF+RMERMVSHSPSFCDITWGAGGSTA
Sbjct: 1   MKVIEKIRAAEAQNQILFSFEYFPPKTDDGVENLFERMERMVSHSPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEIANRMQN VCVETMMHLTCTNMPVEKIDHAL TIK+NGLQNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIANRMQNIVCVETMMHLTCTNMPVEKIDHALTTIKSNGLQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLVNHMRSKYGDYFGIT+AGYPEAHPDAI  NGLAT E+YASDLAYLK
Sbjct: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITIAGYPEAHPDAIGANGLATEEAYASDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           +KVDAGADLIVTQLFYDTD FLKFVNDCRQIGI CPIVPGIMPINNYKGF RMTGFCKTK
Sbjct: 181 RKVDAGADLIVTQLFYDTDNFLKFVNDCRQIGITCPIVPGIMPINNYKGFQRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IPQE+TDALEPIKDN+EAVKAYGIHLGTEMCKKILASGIKQLHLYTLNM+KSALGILLNL
Sbjct: 241 IPQEITDALEPIKDNEEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMEKSALGILLNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKIQRPLPWKRPANVNR KEDVRPIFWANRPKSYI+RTMGWDQYP GRWGDARNA
Sbjct: 301 GLIEESKIQRPLPWKRPANVNRVKEDVRPIFWANRPKSYITRTMGWDQYPHGRWGDARNA 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGALSDHQFMRPRARDKKLQEEWAVPLQ+ E I+EMFKNFCLGKL+SNPWSELDGLQPE
Sbjct: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQSAEAIHEMFKNFCLGKLKSNPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKIIDE+LG+IN KGFLTINSQPAVNGE+SDSPTVGWGGPGGYVYQKAYVEFFC+KEKLN
Sbjct: 421 TKIIDEKLGDINMKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCAKEKLN 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           ALVEKCK+L  ITYMAVNKGGD ISNVAQT VNAVTWG  P+KE+IQPTVVD ASFMIWK
Sbjct: 481 ALVEKCKSLTSITYMAVNKGGDLISNVAQTDVNAVTWGAFPSKEIIQPTVVDSASFMIWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWSRSWA+LYPEGDSSK LLE+VQN+YYLVSLVDNDYI+GDLFSVFT L
Sbjct: 541 DEAFEIWSRSWAQLYPEGDSSKKLLEEVQNSYYLVSLVDNDYIHGDLFSVFTGL 594

[Close]

BLAST of Spo09453.1 vs. UniProtKB/TrEMBL
Match: M1BBU4_SOLTU (Methylenetetrahydrofolate reductase OS=Solanum tuberosum GN=PGSC0003DMG400016168 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 9.700e-310
Identity = 496/594 (83.50%), Postives = 551/594 (92.76%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVI+KI+ +  +++++FSFE+FPPKTDDGVENLF+RMERMV+HSPSFCDITWGAGGSTA
Sbjct: 1   MKVIEKIQEAAKTDKVIFSFEFFPPKTDDGVENLFERMERMVAHSPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTL+I NRMQN VCVE+MMHLTCTNMPVEKIDHALDTIKANG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLDIVNRMQNMVCVESMMHLTCTNMPVEKIDHALDTIKANGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLV HMR+KYGDYFGITVAGYPEAHPD I  NG+AT E Y +DLAYLK
Sbjct: 121 FVQVEGGFACALDLVKHMRAKYGDYFGITVAGYPEAHPDVIPANGIATKEIYENDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KKVDAGAD+IVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGF+RMTGFCKTK
Sbjct: 181 KKVDAGADVIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFLRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP+E+  ALEPIKDN+EAVKAYGIHLGTEMCKKILA+GIK LHLYTLNM+KSAL IL+NL
Sbjct: 241 IPEEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALAILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKI RPLPW+RPANV R KEDVRPIFWANRPKSYISRT GWD+YP GRW +A+N 
Sbjct: 301 GLIEESKISRPLPWRRPANVFRVKEDVRPIFWANRPKSYISRTTGWDEYPHGRWSNAQNP 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGAL+D+QFMR R+RDKKLQEEWAV L ++EDIYE FK++CLGKLRS PWSELDGLQPE
Sbjct: 361 SYGALTDYQFMRARSRDKKLQEEWAVALNSVEDIYEKFKDYCLGKLRSCPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKII+E+LG +N KGFLTINSQPAVN E+SDSP+VGWGGPGGYVYQKAY+E FCS+EKLN
Sbjct: 421 TKIINEKLGQVNTKGFLTINSQPAVNAEKSDSPSVGWGGPGGYVYQKAYLELFCSQEKLN 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           ALVEKCK+  ++TYMAVNK G+WISNV QT VNAVTWGV PAKE+IQPTVVDPASFM+WK
Sbjct: 481 ALVEKCKSFPYLTYMAVNKEGNWISNVNQTDVNAVTWGVFPAKEIIQPTVVDPASFMVWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWSR WA+LYPE D S+ LLEQVQN+Y+LVSLVDNDYINGDLFS+F D+
Sbjct: 541 DEAFEIWSRGWAQLYPETDPSRKLLEQVQNSYFLVSLVDNDYINGDLFSIFKDI 594

[Close]

BLAST of Spo09453.1 vs. UniProtKB/TrEMBL
Match: K4D5E7_SOLLC (Methylenetetrahydrofolate reductase OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 2.200e-309
Identity = 494/594 (83.16%), Postives = 549/594 (92.42%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVI+KI+ +  +++++FSFE+FPPKTDDGVENLF+RMERMV+H PSFCDITWGAGGSTA
Sbjct: 1   MKVIEKIQEAAKTDKVIFSFEFFPPKTDDGVENLFERMERMVAHGPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEI NRMQN VCVE+MMHLTCTNMPVEKIDHALDTIKANG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIVNRMQNMVCVESMMHLTCTNMPVEKIDHALDTIKANGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLV HMR+KYGDYFGITVAGYPEAHPD I  NG+AT E Y +DLAYLK
Sbjct: 121 FVQVEGGFACALDLVKHMRAKYGDYFGITVAGYPEAHPDVIPANGIATKEIYENDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KKVDAGAD+IVTQLFYDTD+FLKFVNDCRQIGINCPIVPGIMPINNYKGF+RMTGFCKTK
Sbjct: 181 KKVDAGADVIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPINNYKGFLRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP E+  ALEPIKDN+EAVKAYGIHLGTEMCKKILA+GIK LHLYTLNM+KSAL IL+NL
Sbjct: 241 IPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALAILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKI RPLPW+RPAN+ R KEDVRPIFWANRPKSYISRT GWD+YP GRW +A+N 
Sbjct: 301 GLIEESKISRPLPWRRPANIFRVKEDVRPIFWANRPKSYISRTTGWDEYPHGRWSNAQNP 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGAL+D+QFMR R+RDKKLQEEWA  L ++EDIYE FK++CLGKLRS PWSELDGLQPE
Sbjct: 361 SYGALTDYQFMRARSRDKKLQEEWAAALNSVEDIYEKFKDYCLGKLRSCPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKII+E+LG +N KGFLTINSQPAVN E+SDSP+VGWGGPGGYVYQKAY+EFFCS+EKLN
Sbjct: 421 TKIINEKLGQVNTKGFLTINSQPAVNAEKSDSPSVGWGGPGGYVYQKAYLEFFCSQEKLN 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           ALVEKCK+  ++TYMAVNK G+WISNV QT +NAVTWGV PAKE+IQPTVVDPASFMIWK
Sbjct: 481 ALVEKCKSFPYLTYMAVNKEGNWISNVNQTDINAVTWGVFPAKEIIQPTVVDPASFMIWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWSR WA+LYPE D S+ LLEQVQN+Y+LVSLVDNDYINGDLFS+F D+
Sbjct: 541 DEAFEIWSRGWAQLYPETDPSRKLLEQVQNSYFLVSLVDNDYINGDLFSIFKDI 594

[Close]

BLAST of Spo09453.1 vs. UniProtKB/TrEMBL
Match: J7EQ04_NICSY (Methylenetetrahydrofolate reductase OS=Nicotiana sylvestris PE=2 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 7.000e-308
Identity = 493/595 (82.86%), Postives = 549/595 (92.27%), Query Frame = 1


		  
Query: 1   MKVIDKIK-ASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGST 60
           MKVI+KI+ A++  N+++FSFE+FPPKT+DGVENLF+RMERMV+H+PSFCDITWGAGGST
Sbjct: 1   MKVIEKIQEAAKDENRVVFSFEFFPPKTEDGVENLFERMERMVAHNPSFCDITWGAGGST 60

Query: 61  ADLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQD 120
           ADLTLEI+ RMQN VCVETMMHLTCTNMPVEKIDHALDTIK NG+QNVLALRGDPPHGQD
Sbjct: 61  ADLTLEISKRMQNMVCVETMMHLTCTNMPVEKIDHALDTIKINGIQNVLALRGDPPHGQD 120

Query: 121 KFVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYL 180
           KFVQVEGGFACALDLV H+R+KYGDYFGITVAGYPEAHPD I  NG+AT E Y +DLAYL
Sbjct: 121 KFVQVEGGFACALDLVKHIRAKYGDYFGITVAGYPEAHPDVIPANGIATQEIYENDLAYL 180

Query: 181 KKKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKT 240
           K+KVDAGADLIVTQLFYDTD+FLKFVNDCRQIGI CPIVPGIMPINNYKGF+RMTGFCKT
Sbjct: 181 KRKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKT 240

Query: 241 KIPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLN 300
           KIP+E+  ALEPIKDN+EAVKAYGIHLGTEMCKKILASGIK LHLYTLNM KSAL IL+N
Sbjct: 241 KIPEEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILASGIKTLHLYTLNMKKSALSILMN 300

Query: 301 LGLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARN 360
           LGLIEESKI RPLPW+RP NV R KEDVRPIFWANRPKSYISRT+GWD+YP GRWG+A+N
Sbjct: 301 LGLIEESKISRPLPWRRPTNVFRVKEDVRPIFWANRPKSYISRTLGWDEYPHGRWGNAQN 360

Query: 361 ASYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQP 420
            SYGALSD+QFMR R+RDKKLQEEW V L ++ED YE FK++CLGKLRS PWSELDGLQP
Sbjct: 361 PSYGALSDYQFMRARSRDKKLQEEWVVALNSVEDTYEKFKDYCLGKLRSCPWSELDGLQP 420

Query: 421 ETKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKL 480
           ETKII+E+LG++N KGFLTINSQPAVN E+SDSP+VGWGGPGGYVYQKAY+EFFCS EKL
Sbjct: 421 ETKIINEKLGHVNTKGFLTINSQPAVNAEKSDSPSVGWGGPGGYVYQKAYLEFFCSGEKL 480

Query: 481 NALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIW 540
           N LVEKCKA  ++TYMAVNK G+WISN++QT VNAVTWGV PAKE++QPTVVDPASFM+W
Sbjct: 481 NTLVEKCKAFPYLTYMAVNKEGNWISNISQTDVNAVTWGVFPAKEIVQPTVVDPASFMVW 540

Query: 541 KDEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           KDEAFEIWSR WA+LYPE D S+ LLEQVQN+YYLVSLVDNDYINGDLFS+F D+
Sbjct: 541 KDEAFEIWSRGWAQLYPETDPSRKLLEQVQNSYYLVSLVDNDYINGDLFSIFQDI 595

[Close]

BLAST of Spo09453.1 vs. ExPASy Swiss-Prot
Match: MTHR2_ARATH (Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana GN=MTHFR2 PE=1 SV=2)

HSP 1 Score: 1040.0 bits (2688), Expect = 9.800e-303
Identity = 479/594 (80.64%), Postives = 541/594 (91.08%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVIDKI++     +  FSFE+FPPKT+DGV+NLF+RM+RMV++ P+FCDITWGAGGSTA
Sbjct: 1   MKVIDKIQSLADEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTL+IA+RMQN VCVE+MMHLTCTNMPVEKIDHAL+TI++NG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGF CALDLVNH+RSKYGDYFGITVAGYPEAHPD I ENGLA+ E+Y SDL YLK
Sbjct: 121 FVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KK+DAGADLIVTQLFYDTD+FLKFVNDCRQIGI+CPIVPGIMPINNY+GF+RMTGFCKTK
Sbjct: 181 KKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP EV  ALEPIKDN+EAVKAYGIHLGTEMCKK+LA G+K LHLYTLNM+KSAL IL+NL
Sbjct: 241 IPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           G+I+ESKI R LPW+RPANV RTKEDVRPIFWANRPKSYISRT GW+ +PQGRWGD+R+A
Sbjct: 301 GMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSRSA 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGALSDHQF RPRARDKKLQ+EW VPL+++EDI E FK  CLG L+S+PWSELDGLQPE
Sbjct: 361 SYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           T+II+EQL  +N KGFLTINSQP+VN ERSDSPTVGWGGP GYVYQKAY+EFFCSKEKL+
Sbjct: 421 TRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEKLD 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           A+VEKCKAL  ITYMAVNKG  W+SN AQ  VNAVTWGV PAKE+IQPT+VDPASF +WK
Sbjct: 481 AVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFE WSRSWA LYPE D S+ LLE+V+N+YYLVSLV+NDYINGD+F+VF DL
Sbjct: 541 DEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFADL 594

[Close]

BLAST of Spo09453.1 vs. ExPASy Swiss-Prot
Match: MTHR1_ARATH (Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana GN=MTHFR1 PE=1 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 2.300e-299
Identity = 472/591 (79.86%), Postives = 532/591 (90.02%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKV+DKIK+     Q  FSFE+FPPKT+DGVENLF+RM+R+VS+ P+FCDITWGAGGSTA
Sbjct: 1   MKVVDKIKSVTEQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEIA+RMQN +CVETMMHLTCTNMP+EKIDHAL+TI++NG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLVNH+RSKYGDYFGITVAGYPEAHPD IE +GLATPESY SDLAYLK
Sbjct: 121 FVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KKVDAGADLIVTQLFYDTD+FLKFVNDCRQIGINCPIVPGIMPI+NYKGF+RM GFCKTK
Sbjct: 181 KKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP E+T ALEPIKDNDEAVKAYGIH  TEMCKKILA GI  LHLYTLN+DKSA+GIL+NL
Sbjct: 241 IPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLI+ESKI R LPW+RPANV RTKEDVRPIFWANRPKSYISRT GW+ +P GRWGD+ +A
Sbjct: 301 GLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSHSA 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           +Y  LSD+QF RP+ RDKKLQ+EW VPL++IED+ E FK  C+G L+S+PWSELDGLQPE
Sbjct: 361 AYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKII+EQLG IN  GFLTINSQP+VN  +SDSP +GWGGPGGYVYQKAY+EFFCSK+KL+
Sbjct: 421 TKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDKLD 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
            LVEK KA   ITYMAVNK  +W+SN  ++ VNAVTWGV PAKEVIQPT+VDPASF +WK
Sbjct: 481 TLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVF 592
           DEAFEIWSRSWA LYPE D S+ LLE+V+N+YYLVSLVDN+YINGD+FSVF
Sbjct: 541 DEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSVF 591

[Close]

BLAST of Spo09453.1 vs. ExPASy Swiss-Prot
Match: MTHR1_MAIZE (Methylenetetrahydrofolate reductase 1 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 1.900e-298
Identity = 473/591 (80.03%), Postives = 531/591 (89.85%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVI+KI  +    +  FSFEYFPPKT++GVENLF+RM+RMV+H PSFCDITWGAGGSTA
Sbjct: 1   MKVIEKILEAAGDGRTAFSFEYFPPKTEEGVENLFERMDRMVAHGPSFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEIANRMQN VCVETMMHLTCTNMPVEKIDHAL+TIK+NG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLV H+R+KYGDYFGITVAGYPEAHPDAI+  G AT E+Y++DLAYLK
Sbjct: 121 FVQVEGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQGEGGATLEAYSNDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           +KVDAGADLIVTQLFYDTD+FLKFVNDCRQIGI CPIVPGIMPINNYKGF+RMTGFCKTK
Sbjct: 181 RKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP E+T AL+PIKDN+EAV+ YGIHLGTEMCKKILA+GIK LHLYTLNMDKSA+GIL+NL
Sbjct: 241 IPSEITAALDPIKDNEEAVRQYGIHLGTEMCKKILATGIKTLHLYTLNMDKSAIGILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESK+ RPLPW+   NV R KEDVRPIFWANRPKSY+ RT+GWDQYP GRWGD+RN 
Sbjct: 301 GLIEESKVSRPLPWRPATNVFRVKEDVRPIFWANRPKSYLKRTLGWDQYPHGRWGDSRNP 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGAL+DHQF RPR R KKLQEEWAVPL+++EDI E F NFC GKL S+PWSELDGLQPE
Sbjct: 361 SYGALTDHQFTRPRGRGKKLQEEWAVPLKSVEDISERFTNFCQGKLTSSPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKIID+QL NIN+KGFLTINSQPAVNGE+SDSPTVGWGGPGGYVYQKAY+EFFC+KEKL+
Sbjct: 421 TKIIDDQLVNINQKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEKLD 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
            L+EK KA   +TY+AVNK G+  SN++   VNAVTWGV P KE+IQPTVVD ASFM+WK
Sbjct: 481 QLIEKIKAFPSLTYIAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMVWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVF 592
           DEAFEIW+R W  ++PEGDSS+ LLE+VQ  YYLVSLVDNDY+ GDLF+ F
Sbjct: 541 DEAFEIWTRGWGCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQGDLFAAF 591

[Close]

BLAST of Spo09453.1 vs. ExPASy Swiss-Prot
Match: MTHR_ORYSJ (Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica GN=Os03g0815200 PE=2 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 4.000e-296
Identity = 470/594 (79.12%), Postives = 535/594 (90.07%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVI+KI+ + +  + +FSFEYFPPKT++G++NLF+RM+RMV+H P+FCDITWGAGGSTA
Sbjct: 1   MKVIEKIQEAAADGRTVFSFEYFPPKTEEGLDNLFERMDRMVAHGPNFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEIANRMQN VCVETMMHLTCTNMPVEKID AL TIK+NG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDDALTTIKSNGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQV GGFACALDLV H+R+KYGDYFGITVAGYPEAHPDAI+    ATPE+Y++DLAYLK
Sbjct: 121 FVQVAGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQSTEGATPEAYSNDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           +KVDAGADLI+TQLFYDTD+FLKFVNDCRQIGI CPIVPGIMPINNYKGF+RMTGFCKTK
Sbjct: 181 QKVDAGADLIITQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP E+T ALEPIKDN+EAVKAYGIHLGTEMCKKILA+GIK LHLYTLNM+KSALGIL+NL
Sbjct: 241 IPAEITAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALGILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLIEESKI R LPW+ P NV R KEDVRPIFWANRPKSYISRT+GWDQYP GRWGD+RN 
Sbjct: 301 GLIEESKISRSLPWRPPTNVFRVKEDVRPIFWANRPKSYISRTLGWDQYPHGRWGDSRNP 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGAL+D+QF RPR R KKLQEEWAVP++++EDI E F NFC GKL S+PWSELDGLQPE
Sbjct: 361 SYGALTDYQFTRPRGRGKKLQEEWAVPVKSVEDINERFMNFCQGKLTSSPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKIID+QL  IN+KGFLTINSQPAVNGERSDS +VGWGGPGGYVYQKAY+EFFCSKEKL+
Sbjct: 421 TKIIDDQLVKINQKGFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEKLD 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
            L+EK KA   +TY+AVNK G+  SN+    VNAVTWGV P KE++QPTVVD ASFM+WK
Sbjct: 481 QLIEKSKAFPSLTYIAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMVWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFEIWS+ WA L+PEGDSS+ +L++VQ +Y+LVSLVDNDYINGDLF+ F ++
Sbjct: 541 DEAFEIWSKGWACLFPEGDSSREILDKVQKSYFLVSLVDNDYINGDLFAAFKEI 594

[Close]

BLAST of Spo09453.1 vs. ExPASy Swiss-Prot
Match: MTHR_HUMAN (Methylenetetrahydrofolate reductase OS=Homo sapiens GN=MTHFR PE=1 SV=3)

HSP 1 Score: 538.1 bits (1385), Expect = 1.200e-151
Identity = 264/605 (43.64%), Postives = 384/605 (63.47%), Query Frame = 1


		  
Query: 5   DKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTAD--- 64
           +K++    S    FS E+FPP+T +G  NL  R +RM +  P + D+TW   G       
Sbjct: 47  EKMRRRLESGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPGSDKE 106

Query: 65  -LTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 124
             ++ IA+   N   +ET++H+TC    +E+I   L   K  GL+N++ALRGDP    D+
Sbjct: 107 TSSMMIASTAVNYCGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDPIG--DQ 166

Query: 125 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 184
           + + EGGF  A+DLV H+RS++GDYF I VAGYP+ HP+A          S+ +DL +LK
Sbjct: 167 WEEEEGGFNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEA---------GSFEADLKHLK 226

Query: 185 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 244
           +KV AGAD I+TQLF++ D F +FV  C  +GI CPIVPGI PI  Y    ++    K +
Sbjct: 227 EKVSAGADFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLE 286

Query: 245 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASG-IKQLHLYTLNMDKSALGILLN 304
           +PQE+ D +EPIKDND A++ YGI L   +C+++LASG +  LH YTLN + +   +L  
Sbjct: 287 VPQEIKDVIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKR 346

Query: 305 LGLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARN 364
           LG+  E   +RPLPW   A+  R +EDVRPIFWA+RPKSYI RT  WD++P GRWG++ +
Sbjct: 347 LGMWTEDP-RRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSS 406

Query: 365 ASYGALSDHQ--FMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSN-------P 424
            ++G L D+   +++ ++  ++L + W   L + E ++E+F  +  G+   N       P
Sbjct: 407 PAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLP 466

Query: 425 WSELDGLQPETKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYV 484
           W++ + L  ET ++ E+L  +N++G LTINSQP +NG+ S  P VGWG  GGYV+QKAY+
Sbjct: 467 WND-EPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYL 526

Query: 485 EFFCSKEKLNALVEKCKALK-HITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPT 544
           EFF S+E   AL++  K  +  + Y  VN  G+ I+N  +   NAVTWG+ P +E+IQPT
Sbjct: 527 EFFTSRETAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPT 586

Query: 545 VVDPASFMIWKDEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDY-INGDLF 594
           VVDP SFM WKDEAF +W   W KLY E   S+T+++ + +NY+LV+LVDND+ ++  L+
Sbjct: 587 VVDPVSFMFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLW 638

[Close]

BLAST of Spo09453.1 vs. TAIR (Arabidopsis)
Match: AT2G44160.1 (methylenetetrahydrofolate reductase 2)

HSP 1 Score: 1040.0 bits (2688), Expect = 5.500e-304
Identity = 479/594 (80.64%), Postives = 541/594 (91.08%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKVIDKI++     +  FSFE+FPPKT+DGV+NLF+RM+RMV++ P+FCDITWGAGGSTA
Sbjct: 1   MKVIDKIQSLADEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTL+IA+RMQN VCVE+MMHLTCTNMPVEKIDHAL+TI++NG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGF CALDLVNH+RSKYGDYFGITVAGYPEAHPD I ENGLA+ E+Y SDL YLK
Sbjct: 121 FVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KK+DAGADLIVTQLFYDTD+FLKFVNDCRQIGI+CPIVPGIMPINNY+GF+RMTGFCKTK
Sbjct: 181 KKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP EV  ALEPIKDN+EAVKAYGIHLGTEMCKK+LA G+K LHLYTLNM+KSAL IL+NL
Sbjct: 241 IPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           G+I+ESKI R LPW+RPANV RTKEDVRPIFWANRPKSYISRT GW+ +PQGRWGD+R+A
Sbjct: 301 GMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSRSA 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           SYGALSDHQF RPRARDKKLQ+EW VPL+++EDI E FK  CLG L+S+PWSELDGLQPE
Sbjct: 361 SYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           T+II+EQL  +N KGFLTINSQP+VN ERSDSPTVGWGGP GYVYQKAY+EFFCSKEKL+
Sbjct: 421 TRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEKLD 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
           A+VEKCKAL  ITYMAVNKG  W+SN AQ  VNAVTWGV PAKE+IQPT+VDPASF +WK
Sbjct: 481 AVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVFTDL 595
           DEAFE WSRSWA LYPE D S+ LLE+V+N+YYLVSLV+NDYINGD+F+VF DL
Sbjct: 541 DEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFADL 594

[Close]

BLAST of Spo09453.1 vs. TAIR (Arabidopsis)
Match: AT3G59970.3 (methylenetetrahydrofolate reductase 1)

HSP 1 Score: 1028.9 bits (2659), Expect = 1.300e-300
Identity = 472/591 (79.86%), Postives = 532/591 (90.02%), Query Frame = 1


		  
Query: 1   MKVIDKIKASESSNQILFSFEYFPPKTDDGVENLFDRMERMVSHSPSFCDITWGAGGSTA 60
           MKV+DKIK+     Q  FSFE+FPPKT+DGVENLF+RM+R+VS+ P+FCDITWGAGGSTA
Sbjct: 1   MKVVDKIKSVTEQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGSTA 60

Query: 61  DLTLEIANRMQNTVCVETMMHLTCTNMPVEKIDHALDTIKANGLQNVLALRGDPPHGQDK 120
           DLTLEIA+RMQN +CVETMMHLTCTNMP+EKIDHAL+TI++NG+QNVLALRGDPPHGQDK
Sbjct: 61  DLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQDK 120

Query: 121 FVQVEGGFACALDLVNHMRSKYGDYFGITVAGYPEAHPDAIEENGLATPESYASDLAYLK 180
           FVQVEGGFACALDLVNH+RSKYGDYFGITVAGYPEAHPD IE +GLATPESY SDLAYLK
Sbjct: 121 FVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAYLK 180

Query: 181 KKVDAGADLIVTQLFYDTDVFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMTGFCKTK 240
           KKVDAGADLIVTQLFYDTD+FLKFVNDCRQIGINCPIVPGIMPI+NYKGF+RM GFCKTK
Sbjct: 181 KKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCKTK 240

Query: 241 IPQEVTDALEPIKDNDEAVKAYGIHLGTEMCKKILASGIKQLHLYTLNMDKSALGILLNL 300
           IP E+T ALEPIKDNDEAVKAYGIH  TEMCKKILA GI  LHLYTLN+DKSA+GIL+NL
Sbjct: 241 IPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILMNL 300

Query: 301 GLIEESKIQRPLPWKRPANVNRTKEDVRPIFWANRPKSYISRTMGWDQYPQGRWGDARNA 360
           GLI+ESKI R LPW+RPANV RTKEDVRPIFWANRPKSYISRT GW+ +P GRWGD+ +A
Sbjct: 301 GLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSHSA 360

Query: 361 SYGALSDHQFMRPRARDKKLQEEWAVPLQNIEDIYEMFKNFCLGKLRSNPWSELDGLQPE 420
           +Y  LSD+QF RP+ RDKKLQ+EW VPL++IED+ E FK  C+G L+S+PWSELDGLQPE
Sbjct: 361 AYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQPE 420

Query: 421 TKIIDEQLGNINKKGFLTINSQPAVNGERSDSPTVGWGGPGGYVYQKAYVEFFCSKEKLN 480
           TKII+EQLG IN  GFLTINSQP+VN  +SDSP +GWGGPGGYVYQKAY+EFFCSK+KL+
Sbjct: 421 TKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDKLD 480

Query: 481 ALVEKCKALKHITYMAVNKGGDWISNVAQTGVNAVTWGVLPAKEVIQPTVVDPASFMIWK 540
            LVEK KA   ITYMAVNK  +W+SN  ++ VNAVTWGV PAKEVIQPT+VDPASF +WK
Sbjct: 481 TLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWK 540

Query: 541 DEAFEIWSRSWAKLYPEGDSSKTLLEQVQNNYYLVSLVDNDYINGDLFSVF 592
           DEAFEIWSRSWA LYPE D S+ LLE+V+N+YYLVSLVDN+YINGD+FSVF
Sbjct: 541 DEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSVF 591

The following BLAST results are available for this feature:

BLAST of Spo09453.1 vs. NCBI nr
BLAST of Spo09453.1 vs. UniProtKB/TrEMBL
BLAST of Spo09453.1 vs. ExPASy Swiss-Prot
BLAST of Spo09453.1 vs. TAIR (Arabidopsis)

BLAST of Spo09453.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902194486\|gb\|KNA12569.1\|	0.0e+0	99.8	hypothetical protein SOVF_1247...	[more]
gi\|731323657\|ref\|XP_010672546.1\|	0.0e+0	91.5	PREDICTED: methylenetetrahydro...	[more]
gi\|470101473\|ref\|XP_004287197.1\|	9.2e-311	83.6	PREDICTED: methylenetetrahydro...	[more]
gi\|565373349\|ref\|XP_006353237.1\|	1.4e-309	83.5	PREDICTED: methylenetetrahydro...	[more]
gi\|970062966\|ref\|XP_015058397.1\|	3.1e-309	83.1	PREDICTED: methylenetetrahydro...	[more]

BLAST of Spo09453.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QZ74_SPIOL	0.0e+0	99.8	Methylenetetrahydrofolate redu...	[more]
A0A0J8CU87_BETVU	0.0e+0	91.5	Methylenetetrahydrofolate redu...	[more]
M1BBU4_SOLTU	9.7e-310	83.5	Methylenetetrahydrofolate redu...	[more]
K4D5E7_SOLLC	2.2e-309	83.1	Methylenetetrahydrofolate redu...	[more]
J7EQ04_NICSY	7.0e-308	82.8	Methylenetetrahydrofolate redu...	[more]

BLAST of Spo09453.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MTHR2_ARATH	9.8e-303	80.6	Methylenetetrahydrofolate redu...	[more]
MTHR1_ARATH	2.3e-299	79.8	Methylenetetrahydrofolate redu...	[more]
MTHR1_MAIZE	1.9e-298	80.0	Methylenetetrahydrofolate redu...	[more]
MTHR_ORYSJ	4.0e-296	79.1	Probable methylenetetrahydrofo...	[more]
MTHR_HUMAN	1.2e-151	43.6	Methylenetetrahydrofolate redu...	[more]

BLAST of Spo09453.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G44160.1	5.5e-304	80.6	methylenetetrahydrofolate redu...	[more]
AT3G59970.3	1.3e-300	79.8	methylenetetrahydrofolate redu...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003171	Methylenetetrahydrofolate reductase	PFAM	PF02219	MTHFR	coord: 8..301 score: 6.7E
IPR004621	Eukaryotic-type methylenetetrahydrofolate reductase	TIGRFAMs	TIGR00677	TIGR00677	coord: 18..305 score: 6.8E
None	No IPR available	PANTHER	PTHR21091	METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED	coord: 129..147 score: 1.3E-245coord: 178..593 score: 1.3E
None	No IPR available	PANTHER	PTHR21091:SF31	METHYLENETETRAHYDROFOLATE REDUCTASE	coord: 129..147 score: 1.3E-245coord: 178..593 score: 1.3E

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006555	methionine metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0010043	response to zinc ion
biological_process	GO:0009744	response to sucrose
biological_process	GO:0033591	response to L-ascorbic acid
biological_process	GO:0046680	response to DDT
biological_process	GO:0046686	response to cadmium ion
biological_process	GO:0009646	response to absence of light
biological_process	GO:0006013	mannose metabolic process
biological_process	GO:0019853	L-ascorbic acid biosynthetic process
biological_process	GO:0009298	GDP-mannose biosynthetic process
biological_process	GO:0006000	fructose metabolic process
biological_process	GO:0035999	tetrahydrofolate interconversion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004489	methylenetetrahydrofolate reductase (NAD(P)H) activity
molecular_function	GO:0004476	mannose-6-phosphate isomerase activity
molecular_function	GO:0008270	zinc ion binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo16188	0.73	Barchart \| Table
Spo10284	0.73	Barchart \| Table
Spo21215	0.71	Barchart \| Table
Spo23258	0.68	Barchart \| Table
Spo00199	0.68	Barchart \| Table
Spo13645	0.68	Barchart \| Table
Spo25493	0.66	Barchart \| Table
Spo01135	0.66	Barchart \| Table
Spo12823	0.66	Barchart \| Table
Spo19595	0.66	Barchart \| Table
Spo22272	0.66	Barchart \| Table
Spo24425	0.66	Barchart \| Table
Spo01925	0.65	Barchart \| Table
Spo18231	0.65	Barchart \| Table