Spo08859 (gene)

Overview
NameSpo08859
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionMethionyl-tRNA synthetase
LocationSpoScf_01862 : 46161 .. 46367 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGTGTTTTTGCTTCCTAGTAGATCAAAAGCGTCAAGTAAGGAAATCAAAACCAGCTGCGGGGACGTGTTCACGGTGCGGTGGCGGTGCTAGCGTTGCGGATATGAAGACAGCCACTAGGTTTTGTCATGTCCCCTTTTACTGGAAGTCTTGGAGAGCTATTATGTGTACCTTTTGTGGATCAATCCTCAAGTCTTACCATTGA

mRNA sequence

ATGGTGTGTTTTTGCTTCCTAGTAGATCAAAAGCGTCAAGTAAGGAAATCAAAACCAGCTGCGGGGACGTGTTCACGGTGCGGTGGCGGTGCTAGCGTTGCGGATATGAAGACAGCCACTAGGTTTTGTCATGTCCCCTTTTACTGGAAGTCTTGGAGAGCTATTATGTGTACCTTTTGTGGATCAATCCTCAAGTCTTACCATTGA

Coding sequence (CDS)

ATGGTGTGTTTTTGCTTCCTAGTAGATCAAAAGCGTCAAGTAAGGAAATCAAAACCAGCTGCGGGGACGTGTTCACGGTGCGGTGGCGGTGCTAGCGTTGCGGATATGAAGACAGCCACTAGGTTTTGTCATGTCCCCTTTTACTGGAAGTCTTGGAGAGCTATTATGTGTACCTTTTGTGGATCAATCCTCAAGTCTTACCATTGA

Protein sequence

MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFCGSILKSYH
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo08859.1Spo08859.1mRNA


Homology
[Close]
BLAST of Spo08859.1 vs. NCBI nr
Match: gi|731356487|ref|XP_010689698.1| (PREDICTED: uncharacterized protein LOC104903371 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 146.4 bits (368), Expect = 1.900e-32
Identity = 63/68 (92.65%), Postives = 68/68 (100.00%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ+RQVR+SKPAAGTC+RCGGGASVADMKTATRFCHVPFYWKSWRAI+CTFC
Sbjct: 1  MVCFCFLVDQQRQVRRSKPAAGTCTRCGGGASVADMKTATRFCHVPFYWKSWRAIICTFC 60

Query: 61 GSILKSYH 69
          GSIL+SYH
Sbjct: 61 GSILRSYH 68
[Close]
BLAST of Spo08859.1 vs. NCBI nr
Match: gi|661868824|emb|CDP21659.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 140.6 bits (353), Expect = 1.000e-30
Identity = 60/68 (88.24%), Postives = 66/68 (97.06%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ R+VR+SKPAAG CSRCGGGASVADMKTATRFCHVPFYWK+WRAI+CTFC
Sbjct: 1  MVCFCFLVDQTRKVRRSKPAAGICSRCGGGASVADMKTATRFCHVPFYWKTWRAIICTFC 60

Query: 61 GSILKSYH 69
          G+IL+SYH
Sbjct: 61 GAILRSYH 68
[Close]
BLAST of Spo08859.1 vs. NCBI nr
Match: gi|566243887|ref|XP_006371597.1| (hypothetical protein POPTR_0019s13510g [Populus trichocarpa])

HSP 1 Score: 140.2 bits (352), Expect = 1.300e-30
Identity = 60/68 (88.24%), Postives = 65/68 (95.59%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ R+VR+SKPAAG CSRCGGGASVADMKT TRFC+VPFYWKSWRAIMCTFC
Sbjct: 1  MVCFCFLVDQTRKVRRSKPAAGICSRCGGGASVADMKTCTRFCYVPFYWKSWRAIMCTFC 60

Query: 61 GSILKSYH 69
          G++LKSYH
Sbjct: 61 GAVLKSYH 68
[Close]
BLAST of Spo08859.1 vs. NCBI nr
Match: gi|225461345|ref|XP_002284622.1| (PREDICTED: uncharacterized protein LOC100241874 [Vitis vinifera])

HSP 1 Score: 138.3 bits (347), Expect = 5.100e-30
Identity = 60/67 (89.55%), Postives = 65/67 (97.01%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ R+VR+SKPAAGTCSRCG GASVADM+TATRFCHVPFYWKSW+AIMCTFC
Sbjct: 1  MVCFCFLVDQTRKVRRSKPAAGTCSRCGRGASVADMRTATRFCHVPFYWKSWKAIMCTFC 60

Query: 61 GSILKSY 68
          G+ILKSY
Sbjct: 61 GAILKSY 67
[Close]
BLAST of Spo08859.1 vs. NCBI nr
Match: gi|225447245|ref|XP_002272845.1| (PREDICTED: uncharacterized protein LOC100261846 [Vitis vinifera])

HSP 1 Score: 138.3 bits (347), Expect = 5.100e-30
Identity = 61/67 (91.04%), Postives = 64/67 (95.52%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ RQVR SKPAAG CSRCGGGASVADMKTATRFC+VPFYWKSWRAI+CTFC
Sbjct: 1  MVCFCFLVDQTRQVRSSKPAAGICSRCGGGASVADMKTATRFCYVPFYWKSWRAIICTFC 60

Query: 61 GSILKSY 68
          GSIL+SY
Sbjct: 61 GSILRSY 67
[Close]
BLAST of Spo08859.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EGC2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g208810 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.300e-32
Identity = 63/68 (92.65%), Postives = 68/68 (100.00%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ+RQVR+SKPAAGTC+RCGGGASVADMKTATRFCHVPFYWKSWRAI+CTFC
Sbjct: 1  MVCFCFLVDQQRQVRRSKPAAGTCTRCGGGASVADMKTATRFCHVPFYWKSWRAIICTFC 60

Query: 61 GSILKSYH 69
          GSIL+SYH
Sbjct: 61 GSILRSYH 68
[Close]
BLAST of Spo08859.1 vs. UniProtKB/TrEMBL
Match: A0A068VM26_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00006999001 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 7.100e-31
Identity = 60/68 (88.24%), Postives = 66/68 (97.06%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ R+VR+SKPAAG CSRCGGGASVADMKTATRFCHVPFYWK+WRAI+CTFC
Sbjct: 1  MVCFCFLVDQTRKVRRSKPAAGICSRCGGGASVADMKTATRFCHVPFYWKTWRAIICTFC 60

Query: 61 GSILKSYH 69
          G+IL+SYH
Sbjct: 61 GAILRSYH 68
[Close]
BLAST of Spo08859.1 vs. UniProtKB/TrEMBL
Match: B9N297_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0019s13510g PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 9.300e-31
Identity = 60/68 (88.24%), Postives = 65/68 (95.59%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ R+VR+SKPAAG CSRCGGGASVADMKT TRFC+VPFYWKSWRAIMCTFC
Sbjct: 1  MVCFCFLVDQTRKVRRSKPAAGICSRCGGGASVADMKTCTRFCYVPFYWKSWRAIMCTFC 60

Query: 61 GSILKSYH 69
          G++LKSYH
Sbjct: 61 GAVLKSYH 68
[Close]
BLAST of Spo08859.1 vs. UniProtKB/TrEMBL
Match: A0A0V0GQ01_SOLCH (Putative ovule protein (Fragment) OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 6.000e-30
Identity = 60/67 (89.55%), Postives = 65/67 (97.01%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ +QV KSKPAAGTCSRCGGGASVADMKTATRFC+VPFYWK+WRAIMCTFC
Sbjct: 29 MVCFCFLVDQTKQVCKSKPAAGTCSRCGGGASVADMKTATRFCYVPFYWKNWRAIMCTFC 88

Query: 61 GSILKSY 68
          G+IL+SY
Sbjct: 89 GAILRSY 95
[Close]
BLAST of Spo08859.1 vs. UniProtKB/TrEMBL
Match: V4U8T4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017406mg PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 1.000e-29
Identity = 59/67 (88.06%), Postives = 63/67 (94.03%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ R+V K KPAAG CSRCGGGASVADMKTATRFCHVPFYWKSWRAI+CTFC
Sbjct: 1  MVCFCFLVDQTRKVHKCKPAAGICSRCGGGASVADMKTATRFCHVPFYWKSWRAIICTFC 60

Query: 61 GSILKSY 68
          G++LKSY
Sbjct: 61 GAVLKSY 67
[Close]
BLAST of Spo08859.1 vs. TAIR (Arabidopsis)
Match: AT1G52855.1 (unknown protein)

HSP 1 Score: 124.4 bits (311), Expect = 2.700e-29
Identity = 51/67 (76.12%), Postives = 62/67 (92.54%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQ R+V++SKPAAGTCSRCG  AS+ADMKT+TRFC +P YW+SWRA++C+FC
Sbjct: 1  MVCFCFLVDQTRKVKRSKPAAGTCSRCGHCASIADMKTSTRFCFIPIYWRSWRAVVCSFC 60

Query: 61 GSILKSY 68
          GS+LKSY
Sbjct: 61 GSVLKSY 67
[Close]
BLAST of Spo08859.1 vs. TAIR (Arabidopsis)
Match: AT3G15534.1 (unknown protein)

HSP 1 Score: 121.7 bits (304), Expect = 1.700e-28
Identity = 53/67 (79.10%), Postives = 61/67 (91.04%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          MVCFCFLVDQKR+V+ SKPAAGTCSRCG GA VADMKT+TRFC +P Y +SWRAI+C+FC
Sbjct: 1  MVCFCFLVDQKRKVKGSKPAAGTCSRCGRGARVADMKTSTRFCLIPIYCRSWRAIVCSFC 60

Query: 61 GSILKSY 68
          GS+LKSY
Sbjct: 61 GSVLKSY 67
[Close]
BLAST of Spo08859.1 vs. TAIR (Arabidopsis)
Match: AT2G20835.1 (unknown protein)

HSP 1 Score: 112.5 bits (280), Expect = 1.100e-25
Identity = 48/68 (70.59%), Postives = 58/68 (85.29%), Query Frame = 1 

		  

Query: 1  MVCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFC 60
          M  FCFLVDQ+++VR  KPAAG CSRCGGGA VADM+T+TRFC VPFY K+W+AI+C  C
Sbjct: 1  MAIFCFLVDQRKKVRGRKPAAGLCSRCGGGAVVADMRTSTRFCGVPFYRKAWKAIVCHIC 60

Query: 61 GSILKSYH 69
          G++LKSYH
Sbjct: 61 GAVLKSYH 68
[Close]
BLAST of Spo08859.1 vs. TAIR (Arabidopsis)
Match: AT5G10695.1 (unknown protein)

HSP 1 Score: 52.0 bits (123), Expect = 1.700e-7
Identity = 25/63 (39.68%), Postives = 32/63 (50.79%), Query Frame = 1 

		  

Query: 2  VCFCFLVDQKRQVRKSKPAAGTCSRCGGGASVADMKTATRFCHVPFYWKSWRAIMCTFCG 61
          +C  F+ DQ  +V     A G C  CGG   V D+ +  RFC VP   KS R  +C+ CG
Sbjct: 1  MCLVFVCDQDERVIGRYAAPGACPYCGGAVQVVDVNSQWRFCFVPLSNKSKRRHLCSTCG 60

Query: 62 SIL 65
            L
Sbjct: 61 KRL 63
The following BLAST results are available for this feature:
BLAST of Spo08859.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|731356487|ref|XP_010689698.1|1.9e-3292.6PREDICTED: uncharacterized pro... [more]
gi|661868824|emb|CDP21659.1|1.0e-3088.2unnamed protein product [Coffe... [more]
gi|566243887|ref|XP_006371597.1|1.3e-3088.2hypothetical protein POPTR_001... [more]
gi|225461345|ref|XP_002284622.1|5.1e-3089.5PREDICTED: uncharacterized pro... [more]
gi|225447245|ref|XP_002272845.1|5.1e-3091.0PREDICTED: uncharacterized pro... [more]
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BLAST of Spo08859.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0J8EGC2_BETVU1.3e-3292.6Uncharacterized protein OS=Bet... [more]
A0A068VM26_COFCA7.1e-3188.2Uncharacterized protein OS=Cof... [more]
B9N297_POPTR9.3e-3188.2Uncharacterized protein OS=Pop... [more]
A0A0V0GQ01_SOLCH6.0e-3089.5Putative ovule protein (Fragme... [more]
V4U8T4_9ROSI1.0e-2988.0Uncharacterized protein OS=Cit... [more]
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BLAST of Spo08859.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo08859.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4
Match NameE-valueIdentityDescription
AT1G52855.12.7e-2976.1unknown protein[more]
AT3G15534.11.7e-2879.1unknown protein[more]
AT2G20835.11.1e-2570.5unknown protein[more]
AT5G10695.11.7e-739.6unknown protein[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33320FAMILY NOT NAMEDcoord: 1..68
score: 8.1
NoneNo IPR availablePANTHERPTHR33320:SF2SUBFAMILY NOT NAMEDcoord: 1..68
score: 8.1

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0006073 cellular glucan metabolic process
biological_process GO:0071555 cell wall organization
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
biological_process GO:0009069 serine family amino acid metabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
cellular_component GO:0000785 chromatin
cellular_component GO:0016020 membrane
molecular_function GO:0003682 chromatin binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0003677 DNA binding
RNA-Seq Expression