Spo06282 (gene)

Overview
NameSpo06282
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionRalf-like 34, putative
LocationSpoScf_03651 : 5041 .. 5451 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCGAAGACTGTTATCTCTCTCCTCTTTCTGATCTCCTACATTACCCTCCATACTTCCACTGTGGAAGCTCAGATCCACGACTTCGGGTCTGATTGGGCTTCGTCTTCCGCAATGTCGATGGTGTTAAACCCCAGCGGAGTAAGTGGTGGAGGTGGAGGAGATCTTGATCTTGATTTTGATGATGAGATTGATGAAGATGGAATCGACGGTAGTAGCAGTAGTAGGAGATCTCTGTACTGGAAGAGGAAGGTGTACTACATATCGTATGGTGCGTTGTCAGCGAATCGGGTCCCGTGTCCACCACGCTCCGGTAGGTCTTACTACACTCACAACTGTTACGGAACTCATGCCCCTGCTAATCCTTATTCTCGCGGTTGCTCTTGCATCACTCGTTGCCGCCGTTAG

mRNA sequence

ATGTCTTCGAAGACTGTTATCTCTCTCCTCTTTCTGATCTCCTACATTACCCTCCATACTTCCACTGTGGAAGCTCAGATCCACGACTTCGGGTCTGATTGGGCTTCGTCTTCCGCAATGTCGATGGTGTTAAACCCCAGCGGAGTAAGTGGTGGAGGTGGAGGAGATCTTGATCTTGATTTTGATGATGAGATTGATGAAGATGGAATCGACGGTAGTAGCAGTAGTAGGAGATCTCTGTACTGGAAGAGGAAGGTGTACTACATATCGTATGGTGCGTTGTCAGCGAATCGGGTCCCGTGTCCACCACGCTCCGGTAGGTCTTACTACACTCACAACTGTTACGGAACTCATGCCCCTGCTAATCCTTATTCTCGCGGTTGCTCTTGCATCACTCGTTGCCGCCGTTAG

Coding sequence (CDS)

ATGTCTTCGAAGACTGTTATCTCTCTCCTCTTTCTGATCTCCTACATTACCCTCCATACTTCCACTGTGGAAGCTCAGATCCACGACTTCGGGTCTGATTGGGCTTCGTCTTCCGCAATGTCGATGGTGTTAAACCCCAGCGGAGTAAGTGGTGGAGGTGGAGGAGATCTTGATCTTGATTTTGATGATGAGATTGATGAAGATGGAATCGACGGTAGTAGCAGTAGTAGGAGATCTCTGTACTGGAAGAGGAAGGTGTACTACATATCGTATGGTGCGTTGTCAGCGAATCGGGTCCCGTGTCCACCACGCTCCGGTAGGTCTTACTACACTCACAACTGTTACGGAACTCATGCCCCTGCTAATCCTTATTCTCGCGGTTGCTCTTGCATCACTCGTTGCCGCCGTTAG

Protein sequence

MSSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLDFDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPYSRGCSCITRCRR
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo06282.1Spo06282.1mRNA


Homology
[Close]
BLAST of Spo06282.1 vs. NCBI nr
Match: gi|902173449|gb|KNA08265.1| (hypothetical protein SOVF_164140 [Spinacia oleracea])

HSP 1 Score: 276.6 bits (706), Expect = 2.400e-71
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 1 

		  

Query: 1   MSSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLD 60
           MSSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLD
Sbjct: 1   MSSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLD 60

Query: 61  FDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAP 120
           FDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAP
Sbjct: 61  FDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAP 120

Query: 121 ANPYSRGCSCITRCRR 137
           ANPYSRGCSCITRCRR
Sbjct: 121 ANPYSRGCSCITRCRR 136
[Close]
BLAST of Spo06282.1 vs. NCBI nr
Match: gi|731375925|ref|XP_010667406.1| (PREDICTED: protein RALF-like 34 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 167.2 bits (422), Expect = 2.000e-38
Identity = 90/132 (68.18%), Postives = 100/132 (75.76%), Query Frame = 1 

		  

Query: 8   SLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLD-FDDEID 67
           SLL  ISY++LH ST  AQIHD G +WAS SAMS+V          G DLD D F+DEI+
Sbjct: 12  SLLLTISYLSLHFSTTNAQIHDLGFNWASPSAMSIVYPV-------GNDLDDDYFEDEIE 71

Query: 68  EDGIDGSSSSRRSLYWKR--KVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPY 127
            DG    SSSRRSLYWK+  +VYYISYGALSANRVPCPPRSGRSYYTHNC+ T  PANPY
Sbjct: 72  SDG----SSSRRSLYWKKPHQVYYISYGALSANRVPCPPRSGRSYYTHNCFKTRTPANPY 131

Query: 128 SRGCSCITRCRR 137
            RGCSCI+RCRR
Sbjct: 132 HRGCSCISRCRR 132
[Close]
BLAST of Spo06282.1 vs. NCBI nr
Match: gi|823249662|ref|XP_012457452.1| (PREDICTED: protein RALF-like 34 isoform X2 [Gossypium raimondii])

HSP 1 Score: 125.9 bits (315), Expect = 5.200e-26
Identity = 76/139 (54.68%), Postives = 92/139 (66.19%), Query Frame = 1 

		  

Query: 2   SSKTVISLLFL---ISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLD 61
           SS  ++S L L   I++I L TS VEAQ+ D  S      A+   L  S  S       +
Sbjct: 4   SSSVLLSKLLLPLFITHIVLTTSIVEAQVED-ASLKLVRDALERPLPISSYS-------E 63

Query: 62  LDFDDEIDEDGIDGSSS-SRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGT 121
           L+ D+EID+D  DG +  SRRSL+WKR  YYISYGAL ANR+PCPPRSGRSYYTHNC+  
Sbjct: 64  LNDDEEIDDD--DGENGYSRRSLFWKRMGYYISYGALWANRIPCPPRSGRSYYTHNCFKV 123

Query: 122 HAPANPYSRGCSCITRCRR 137
           H P +PY+RGCS ITRCRR
Sbjct: 124 HGPVHPYTRGCSRITRCRR 132
[Close]
BLAST of Spo06282.1 vs. NCBI nr
Match: gi|823249660|ref|XP_012457450.1| (PREDICTED: protein RALF-like 34 isoform X1 [Gossypium raimondii])

HSP 1 Score: 124.0 bits (310), Expect = 2.000e-25
Identity = 77/142 (54.23%), Postives = 91/142 (64.08%), Query Frame = 1 

		  

Query: 2   SSKTVISLLFL---ISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGG--- 61
           SS  ++S L L   I++I L TS VEAQ+ D  S      A+   L  S  S        
Sbjct: 4   SSSVLLSKLLLPLFITHIVLTTSIVEAQVED-ASLKLVRDALERPLPISSYSELNDDEEI 63

Query: 62  DLDLDFDDEIDEDGIDGSSS-SRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNC 121
           D D D DD+ D+D  DG +  SRRSL+WKR  YYISYGAL ANR+PCPPRSGRSYYTHNC
Sbjct: 64  DDDGDDDDDDDDDDDDGENGYSRRSLFWKRMGYYISYGALWANRIPCPPRSGRSYYTHNC 123

Query: 122 YGTHAPANPYSRGCSCITRCRR 137
           +  H P +PY+RGCS ITRCRR
Sbjct: 124 FKVHGPVHPYTRGCSRITRCRR 144
[Close]
BLAST of Spo06282.1 vs. NCBI nr
Match: gi|1021042075|gb|KZM99856.1| (hypothetical protein DCAR_012782 [Daucus carota subsp. sativus])

HSP 1 Score: 122.9 bits (307), Expect = 4.400e-25
Identity = 73/132 (55.30%), Postives = 85/132 (64.39%), Query Frame = 1 

		  

Query: 7   ISLLFLISYITLHTSTVEAQIHDFGSD--WASSSAMSMVLNPSGVSGGGGGDLDLDFDDE 66
           +SLLFL+  I+  T  V +QI    S+  W           PS  SG  G +L+LD D E
Sbjct: 6   LSLLFLLICISYTTLPVSSQIETLDSNLQW-----------PSASSGYAGVELELDEDQE 65

Query: 67  IDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPY 126
              D IDG+   RRSLYW+   YYISYGALSANR+PCP RSGRSYYTHNC+    PA+PY
Sbjct: 66  ---DVIDGA---RRSLYWRSMHYYISYGALSANRIPCPARSGRSYYTHNCHSATGPAHPY 120

Query: 127 SRGCSCITRCRR 137
           SRGCS ITRCRR
Sbjct: 126 SRGCSAITRCRR 120
[Close]
BLAST of Spo06282.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QLX4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_164140 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 1.700e-71
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 1 

		  

Query: 1   MSSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLD 60
           MSSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLD
Sbjct: 1   MSSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLD 60

Query: 61  FDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAP 120
           FDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAP
Sbjct: 61  FDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAP 120

Query: 121 ANPYSRGCSCITRCRR 137
           ANPYSRGCSCITRCRR
Sbjct: 121 ANPYSRGCSCITRCRR 136
[Close]
BLAST of Spo06282.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B2P8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_008520 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.400e-38
Identity = 90/132 (68.18%), Postives = 100/132 (75.76%), Query Frame = 1 

		  

Query: 8   SLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGGDLDLD-FDDEID 67
           SLL  ISY++LH ST  AQIHD G +WAS SAMS+V          G DLD D F+DEI+
Sbjct: 12  SLLLTISYLSLHFSTTNAQIHDLGFNWASPSAMSIVYPV-------GNDLDDDYFEDEIE 71

Query: 68  EDGIDGSSSSRRSLYWKR--KVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPY 127
            DG    SSSRRSLYWK+  +VYYISYGALSANRVPCPPRSGRSYYTHNC+ T  PANPY
Sbjct: 72  SDG----SSSRRSLYWKKPHQVYYISYGALSANRVPCPPRSGRSYYTHNCFKTRTPANPY 131

Query: 128 SRGCSCITRCRR 137
            RGCSCI+RCRR
Sbjct: 132 HRGCSCISRCRR 132
[Close]
BLAST of Spo06282.1 vs. UniProtKB/TrEMBL
Match: A0A0D2US46_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G105900 PE=4 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.400e-25
Identity = 77/142 (54.23%), Postives = 91/142 (64.08%), Query Frame = 1 

		  

Query: 2   SSKTVISLLFL---ISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSGVSGGGGG--- 61
           SS  ++S L L   I++I L TS VEAQ+ D  S      A+   L  S  S        
Sbjct: 4   SSSVLLSKLLLPLFITHIVLTTSIVEAQVED-ASLKLVRDALERPLPISSYSELNDDEEI 63

Query: 62  DLDLDFDDEIDEDGIDGSSS-SRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNC 121
           D D D DD+ D+D  DG +  SRRSL+WKR  YYISYGAL ANR+PCPPRSGRSYYTHNC
Sbjct: 64  DDDGDDDDDDDDDDDDGENGYSRRSLFWKRMGYYISYGALWANRIPCPPRSGRSYYTHNC 123

Query: 122 YGTHAPANPYSRGCSCITRCRR 137
           +  H P +PY+RGCS ITRCRR
Sbjct: 124 FKVHGPVHPYTRGCSRITRCRR 144
[Close]
BLAST of Spo06282.1 vs. UniProtKB/TrEMBL
Match: A0A165ZC30_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_012782 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 3.100e-25
Identity = 73/132 (55.30%), Postives = 85/132 (64.39%), Query Frame = 1 

		  

Query: 7   ISLLFLISYITLHTSTVEAQIHDFGSD--WASSSAMSMVLNPSGVSGGGGGDLDLDFDDE 66
           +SLLFL+  I+  T  V +QI    S+  W           PS  SG  G +L+LD D E
Sbjct: 6   LSLLFLLICISYTTLPVSSQIETLDSNLQW-----------PSASSGYAGVELELDEDQE 65

Query: 67  IDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPY 126
              D IDG+   RRSLYW+   YYISYGALSANR+PCP RSGRSYYTHNC+    PA+PY
Sbjct: 66  ---DVIDGA---RRSLYWRSMHYYISYGALSANRIPCPARSGRSYYTHNCHSATGPAHPY 120

Query: 127 SRGCSCITRCRR 137
           SRGCS ITRCRR
Sbjct: 126 SRGCSAITRCRR 120
[Close]
BLAST of Spo06282.1 vs. UniProtKB/TrEMBL
Match: A0A078CWV1_BRANA (BnaC09g07510D protein OS=Brassica napus GN=BnaC09g07510D PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 6.900e-25
Identity = 61/98 (62.24%), Postives = 69/98 (70.41%), Query Frame = 1 

		  

Query: 41  SMVLNPSGVSGGGGGDLDLDFDDEIDEDGIDGSS--SSRRSLYWKRKVYYISYGALSANR 100
           S+  NPS +  G G D  +   D  D DG +     + RRSLYWKR+ YYISYGALSANR
Sbjct: 25  SLPQNPSMILLGDGFDWPISHGDAFDIDGGESFEDVADRRSLYWKRRRYYISYGALSANR 84

Query: 101 VPCPPRSGRSYYTHNCYGTHAPANPYSRGCSCITRCRR 137
           VPCPPRSGRSYYTHNC+    P +PYSRGCS ITRCRR
Sbjct: 85  VPCPPRSGRSYYTHNCFRARGPVHPYSRGCSSITRCRR 122
[Close]
BLAST of Spo06282.1 vs. ExPASy Swiss-Prot
Match: RLF34_ARATH (Protein RALF-like 34 OS=Arabidopsis thaliana GN=RALFL34 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 8.300e-24
Identity = 65/139 (46.76%), Postives = 84/139 (60.43%), Query Frame = 1 

		  

Query: 2   SSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSG----VSGGGGGDL 61
           SS  ++ +L L+++I+L  S             + S   S+ L P G    +S     D+
Sbjct: 4   SSLNLLLILSLLTFISLQRSE------------SLSDNPSLTLLPDGFDWPISHSDEFDI 63

Query: 62  DLDFDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGT 121
            +D ++  +    D   + RRSLYW+R  YYISYGALSANRVPCPPRSGRSYYTHNC+  
Sbjct: 64  -IDGEESFEVTEEDDGVTDRRSLYWRRTKYYISYGALSANRVPCPPRSGRSYYTHNCFRA 123

Query: 122 HAPANPYSRGCSCITRCRR 137
             P +PYSRGCS ITRCRR
Sbjct: 124 RGPVHPYSRGCSSITRCRR 129
[Close]
BLAST of Spo06282.1 vs. ExPASy Swiss-Prot
Match: RLF23_ARATH (Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.800e-8
Identity = 53/145 (36.55%), Postives = 70/145 (48.28%), Query Frame = 1 

		  

Query: 2   SSKTVISLLFLISYITLHTSTVEAQIHDFGSDWA----------SSSAMSMVLNPSGVSG 61
           S    I  + LI  +   +  V +Q  +F  D+           +  ++S  L   G   
Sbjct: 8   SGAAAIFAILLILAVHNWSVAVSSQSTEFAGDFPPFETECRGTIAECSVSAALGDGGDLF 67

Query: 62  GGGGDLDLDFDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYT 121
            GGG++  +F+ +        S  +RR L  +R   YISYGAL  N +PC  R G SYY 
Sbjct: 68  YGGGEMGEEFEMD--------SEINRRILATRR---YISYGALRRNTIPCS-RRGASYY- 127

Query: 122 HNCYGTHAPANPYSRGCSCITRCRR 137
            NC    A ANPYSRGCS ITRCRR
Sbjct: 128 -NCR-RGAQANPYSRGCSAITRCRR 137
[Close]
BLAST of Spo06282.1 vs. ExPASy Swiss-Prot
Match: RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.800e-8
Identity = 34/49 (69.39%), Postives = 34/49 (69.39%), Query Frame = 1 

		  

Query: 88  YISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPYSRGCSCITRCRR 137
           YISYGAL  N VPC  R G SYY  NC    A ANPYSRGCS ITRCRR
Sbjct: 72  YISYGALRRNTVPCS-RRGASYY--NCR-RGAQANPYSRGCSAITRCRR 116
[Close]
BLAST of Spo06282.1 vs. ExPASy Swiss-Prot
Match: RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 8.400e-8
Identity = 46/90 (51.11%), Postives = 53/90 (58.89%), Query Frame = 1 

		  

Query: 46  PSGVSGGGGGDLDLDFDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRS 105
           P+   GG  G +     +E +E  +D S S+RR L  K+   YISYGAL  N VPC  R 
Sbjct: 34  PARSGGGCKGSIGECIAEE-EEFELD-SESNRRILATKK---YISYGALQKNSVPCS-RR 93

Query: 106 GRSYYTHNCYGTHAPANPYSRGCSCITRCR 136
           G SYY  NC    A ANPYSRGCS ITRCR
Sbjct: 94  GASYY--NCK-PGAQANPYSRGCSAITRCR 114
[Close]
BLAST of Spo06282.1 vs. ExPASy Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.400e-7
Identity = 53/127 (41.73%), Postives = 68/127 (53.54%), Query Frame = 1 

		  

Query: 12  LISYITLHTSTVEAQIHDFGS--DWASSSAMSMVLNPSGVSGGGGGDLDLDFDDEIDEDG 71
           +I+ + +  S VE+   DFG   D+  + + S+    SG +G     +  + + E D D 
Sbjct: 10  VIAILAIVISAVEST-GDFGDSLDFVRAGSSSLF---SGCTGSIAECIAEEEEMEFDSD- 69

Query: 72  IDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPYSRGCS 131
                 SRR L  K+   YISYGA+  N VPC  R G SYY  NC    A ANPYSRGCS
Sbjct: 70  -----ISRRILAQKK---YISYGAMRRNSVPCS-RRGASYY--NCQ-RGAQANPYSRGCS 119

Query: 132 CITRCRR 137
            ITRCRR
Sbjct: 130 TITRCRR 119
[Close]
BLAST of Spo06282.1 vs. TAIR (Arabidopsis)
Match: AT5G67070.1 (ralf-like 34)

HSP 1 Score: 111.3 bits (277), Expect = 4.700e-25
Identity = 65/139 (46.76%), Postives = 84/139 (60.43%), Query Frame = 1 

		  

Query: 2   SSKTVISLLFLISYITLHTSTVEAQIHDFGSDWASSSAMSMVLNPSG----VSGGGGGDL 61
           SS  ++ +L L+++I+L  S             + S   S+ L P G    +S     D+
Sbjct: 4   SSLNLLLILSLLTFISLQRSE------------SLSDNPSLTLLPDGFDWPISHSDEFDI 63

Query: 62  DLDFDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGT 121
            +D ++  +    D   + RRSLYW+R  YYISYGALSANRVPCPPRSGRSYYTHNC+  
Sbjct: 64  -IDGEESFEVTEEDDGVTDRRSLYWRRTKYYISYGALSANRVPCPPRSGRSYYTHNCFRA 123

Query: 122 HAPANPYSRGCSCITRCRR 137
             P +PYSRGCS ITRCRR
Sbjct: 124 RGPVHPYSRGCSSITRCRR 129
[Close]
BLAST of Spo06282.1 vs. TAIR (Arabidopsis)
Match: AT3G16570.1 (rapid alkalinization factor 23)

HSP 1 Score: 59.3 bits (142), Expect = 2.100e-9
Identity = 53/145 (36.55%), Postives = 70/145 (48.28%), Query Frame = 1 

		  

Query: 2   SSKTVISLLFLISYITLHTSTVEAQIHDFGSDWA----------SSSAMSMVLNPSGVSG 61
           S    I  + LI  +   +  V +Q  +F  D+           +  ++S  L   G   
Sbjct: 8   SGAAAIFAILLILAVHNWSVAVSSQSTEFAGDFPPFETECRGTIAECSVSAALGDGGDLF 67

Query: 62  GGGGDLDLDFDDEIDEDGIDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYT 121
            GGG++  +F+ +        S  +RR L  +R   YISYGAL  N +PC  R G SYY 
Sbjct: 68  YGGGEMGEEFEMD--------SEINRRILATRR---YISYGALRRNTIPCS-RRGASYY- 127

Query: 122 HNCYGTHAPANPYSRGCSCITRCRR 137
            NC    A ANPYSRGCS ITRCRR
Sbjct: 128 -NCR-RGAQANPYSRGCSAITRCRR 137
[Close]
BLAST of Spo06282.1 vs. TAIR (Arabidopsis)
Match: AT4G15800.1 (ralf-like 33)

HSP 1 Score: 59.3 bits (142), Expect = 2.100e-9
Identity = 34/49 (69.39%), Postives = 34/49 (69.39%), Query Frame = 1 

		  

Query: 88  YISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPYSRGCSCITRCRR 137
           YISYGAL  N VPC  R G SYY  NC    A ANPYSRGCS ITRCRR
Sbjct: 72  YISYGALRRNTVPCS-RRGASYY--NCR-RGAQANPYSRGCSAITRCRR 116
[Close]
BLAST of Spo06282.1 vs. TAIR (Arabidopsis)
Match: AT3G05490.1 (ralf-like 22)

HSP 1 Score: 57.4 bits (137), Expect = 8.100e-9
Identity = 53/127 (41.73%), Postives = 68/127 (53.54%), Query Frame = 1 

		  

Query: 12  LISYITLHTSTVEAQIHDFGS--DWASSSAMSMVLNPSGVSGGGGGDLDLDFDDEIDEDG 71
           +I+ + +  S VE+   DFG   D+  + + S+    SG +G     +  + + E D D 
Sbjct: 10  VIAILAIVISAVEST-GDFGDSLDFVRAGSSSLF---SGCTGSIAECIAEEEEMEFDSD- 69

Query: 72  IDGSSSSRRSLYWKRKVYYISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPYSRGCS 131
                 SRR L  K+   YISYGA+  N VPC  R G SYY  NC    A ANPYSRGCS
Sbjct: 70  -----ISRRILAQKK---YISYGAMRRNSVPCS-RRGASYY--NCQ-RGAQANPYSRGCS 119

Query: 132 CITRCRR 137
            ITRCRR
Sbjct: 130 TITRCRR 119
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BLAST of Spo06282.1 vs. TAIR (Arabidopsis)
Match: AT1G02900.1 (rapid alkalinization factor 1)

HSP 1 Score: 51.6 bits (122), Expect = 4.400e-7
Identity = 30/48 (62.50%), Postives = 31/48 (64.58%), Query Frame = 1 

		  

Query: 88  YISYGALSANRVPCPPRSGRSYYTHNCYGTHAPANPYSRGCSCITRCR 136
           YISY +L  N VPC  R G SYY  NC    A ANPYSRGCS I RCR
Sbjct: 76  YISYQSLKRNSVPCS-RRGASYY--NCQNG-AQANPYSRGCSKIARCR 119
The following BLAST results are available for this feature:
BLAST of Spo06282.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902173449|gb|KNA08265.1|2.4e-71100.hypothetical protein SOVF_1641... [more]
gi|731375925|ref|XP_010667406.1|2.0e-3868.1PREDICTED: protein RALF-like 3... [more]
gi|823249662|ref|XP_012457452.1|5.2e-2654.6PREDICTED: protein RALF-like 3... [more]
gi|823249660|ref|XP_012457450.1|2.0e-2554.2PREDICTED: protein RALF-like 3... [more]
gi|1021042075|gb|KZM99856.1|4.4e-2555.3hypothetical protein DCAR_0127... [more]
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BLAST of Spo06282.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QLX4_SPIOL1.7e-71100.Uncharacterized protein OS=Spi... [more]
A0A0J8B2P8_BETVU1.4e-3868.1Uncharacterized protein OS=Bet... [more]
A0A0D2US46_GOSRA1.4e-2554.2Uncharacterized protein OS=Gos... [more]
A0A165ZC30_DAUCA3.1e-2555.3Uncharacterized protein OS=Dau... [more]
A0A078CWV1_BRANA6.9e-2562.2BnaC09g07510D protein OS=Brass... [more]
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BLAST of Spo06282.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
RLF34_ARATH8.3e-2446.7Protein RALF-like 34 OS=Arabid... [more]
RLF23_ARATH3.8e-836.5Rapid alkalinization factor 23... [more]
RLF33_ARATH3.8e-869.3Protein RALF-like 33 OS=Arabid... [more]
RALF_TOBAC8.4e-851.1Rapid alkalinization factor OS... [more]
RLF22_ARATH1.4e-741.7Protein RALF-like 22 OS=Arabid... [more]
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BLAST of Spo06282.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT5G67070.14.7e-2546.7ralf-like 34[more]
AT3G16570.12.1e-936.5rapid alkalinization factor 23[more]
AT4G15800.12.1e-969.3ralf-like 33[more]
AT3G05490.18.1e-941.7ralf-like 22[more]
AT1G02900.14.4e-762.5rapid alkalinization factor 1[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 72..136
score: 1.6
NoneNo IPR availablePANTHERPTHR33136FAMILY NOT NAMEDcoord: 6..136
score: 1.1
NoneNo IPR availablePANTHERPTHR33136:SF7PROTEIN RALF-LIKE 26-RELATEDcoord: 6..136
score: 1.1

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019722 calcium-mediated signaling
biological_process GO:0007267 cell-cell signaling
biological_process GO:0006949 syncytium formation
biological_process GO:0030001 metal ion transport
biological_process GO:0042254 ribosome biogenesis
biological_process GO:0006412 translation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005618 cell wall
cellular_component GO:0005840 ribosome
cellular_component GO:0048046 apoplast
molecular_function GO:0003735 structural constituent of ribosome
molecular_function GO:0004871 obsolete signal transducer activity
molecular_function GO:0005179 hormone activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0005515 protein binding
RNA-Seq Expression