Spo05963 (gene)

Overview
NameSpo05963
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionMitotic spindle checkpoint protein MAD2
Locationchr1 : 49274619 .. 49276779 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCGATTATCCAAAAACCCAGAAAAACTAGCCGTTACCTCTTTTTCTCTTCCCTTTTCTGGGTGGATTTTATTTTAATTCGTCAATTCAATCAACTTTATCCCCAACTTTACAAACCCCACCAAATTAAATTCAATGCACTACACTTAACTTCAATATAAAACCCTTGAATTTCACTTCCATTCATTTTTTCCATCAACACCCACAAAATCAAGAACTCTATAATCTTACAAAAAAGAAAATTCAATGGCTTCTAAAACTGTTGCTAAAGATATCATCACTCTCCGTGGCTCGGCAGCGATCGCCAGCGAATTCTTTGGTAAATTTTTAACGCCATTTTTTCCTTCTATGTCTAATTTTCTTCCAAAAATGAAACTTCCAATCTTTCTTCCATTTTTCTCAACCCATTTTTGTTTGCATTTTTTAGGGTATGCGGCAAACAGGTGAGAAATTCGTCATTTTGGGTAGAAGTTTCATTTTTTTATCGTATTAATTTAATTATTGATTGAGTTGTTTTGATTATTATTAGTATATTGTACAATCGAGGGGTTTACCCAGAAGAAAGTTTTGGGAAAGTGAAGAAATATGGATTGCCAATGCTTCTTACTCAAGATGAGGCTGTTAAAACTTTCATCGCTAATCTTACTGCTCAACTTTCAGGTAAATTTCTTATTTTTATTGTGTTTTTCTCCTTATATTTCCTTCCTACTCATACTTTATTTCGATTTAATTTCTGGGTGGTTCTTGTTAAATTAAATTTGATTTCTGTGGTTTTTGTGGAATTCAAATCATTTTCTAGGGTTCTTGTTAAATTCAATTTAATTTCTGGGGTTTGTGAAATTCAAATCATTTTCTAGGGTTCTTGTTAGGTTCAATTTAATTTCTGAGGTTTTTGTTAAATTAATTTATTTTCTGGGGTTGTCTTAAAATCAAATACTTCGTACTGTACAATTTTCTGGGATTTTGTGAAATTCATTTATTTCTGGGATTTTTGTTGAATTTAATTAATTTTCTGGGTTTTTTTTTTGTTTTGATTTTGTAATGCAGAATGGCTTGAAGCAGGGAAATTACAGAGGGTTGTTTTAGTTATAATGAGCAAATCTACAAATGAAGTTCTGGAAAGATGGAATTTCAGCATTGAAACTGATAATGAAGTTGTTGAAACAGGGTAATTAAAACTAAAAAATCAAGGAATTTCAGCATTGAAACTTTTTTCTTTAATTATTTGGCAAAAAATAAAATAAAAAATGGAATCATCAATTTTATTACTAATTCAATTTTGGGTTTGATTTTATTTCAGGGTATCAAGAGAAAAAAGTGACAAGGAAATAATGAGGGAAATTCAAGCAATTATGAGGCAGATTGCTTCTAGCATCACTTACTTGCCTTGCCTTGATGAACCTTGTAATTATTACACTGATTTTCTTTAATTATATTCCCATTTTTGTATATTCGGGATTAGTTGTCGCATTACTATGTTAGGATGTTCGGAAAATGTTACTCGTATCATAGTTTCCAAAGTAATTTTTACGGTTTTGCCCTTACTAGGACCGGATAATGGGAGAAAGTGTAACTAACTTTTTTTTTTTTTGTAATATTTCTGATTAACCTTTGAAATATGTTGCAGGTGTTTTTGATGTATTGGCATACACAGACAAGGATGTAGATGCACCTATTACTTGGACTGAGAGTGATCCCAAATTGATTTCTAATCCTCAAATGGTGAAACTACATTCTTTTGATACCAAGGTATAAGAAATCAAAACAGAAGCATTGATTTTCTGTAATTGGATTGATTTTTCCTTAAATTTAGATGTACAATTGTTGTTTCTGGGGGTTAAACAGAGTGAATTTTTTGTTGCAGATTCACAAGGTTGACACTTTAGTTTCTTACAAGAATGATGATGATGATGATGATGAATGACAAGCAAGTGAAAGAAAGTTCGATTTTTAGGTGAAAAATGGGATGAAATACTAAGTGTAGTGTGTGTGAATCATTGATTCACAATTTTGTATCTGGGATCTGAAGTTTGTATTGTACTAAGAAGTGTAATGATGAATTACCTGTTAATCATTTCAGTGATTACATTTCTGCCATTGCTCTGTAATACAGTGTAGTTCAGTTGGATTGAAATTTATCCAAAATTTAGCTGATTCTGAAA

mRNA sequence

TCCGATTATCCAAAAACCCAGAAAAACTAGCCGTTACCTCTTTTTCTCTTCCCTTTTCTGGGTGGATTTTATTTTAATTCGTCAATTCAATCAACTTTATCCCCAACTTTACAAACCCCACCAAATTAAATTCAATGCACTACACTTAACTTCAATATAAAACCCTTGAATTTCACTTCCATTCATTTTTTCCATCAACACCCACAAAATCAAGAACTCTATAATCTTACAAAAAAGAAAATTCAATGGCTTCTAAAACTGTTGCTAAAGATATCATCACTCTCCGTGGCTCGGCAGCGATCGCCAGCGAATTCTTTGGGTATGCGGCAAACAGTATATTGTACAATCGAGGGGTTTACCCAGAAGAAAGTTTTGGGAAAGTGAAGAAATATGGATTGCCAATGCTTCTTACTCAAGATGAGGCTGTTAAAACTTTCATCGCTAATCTTACTGCTCAACTTTCAGAATGGCTTGAAGCAGGGAAATTACAGAGGGTTGTTTTAGTTATAATGAGCAAATCTACAAATGAAGTTCTGGAAAGATGGAATTTCAGCATTGAAACTGATAATGAAGTTGTTGAAACAGGGGTATCAAGAGAAAAAAGTGACAAGGAAATAATGAGGGAAATTCAAGCAATTATGAGGCAGATTGCTTCTAGCATCACTTACTTGCCTTGCCTTGATGAACCTTGTGTTTTTGATGTATTGGCATACACAGACAAGGATGTAGATGCACCTATTACTTGGACTGAGAGTGATCCCAAATTGATTTCTAATCCTCAAATGGTGAAACTACATTCTTTTGATACCAAGATTCACAAGGTTGACACTTTAGTTTCTTACAAGAATGATGATGATGATGATGATGAATGACAAGCAAGTGAAAGAAAGTTCGATTTTTAGGTGAAAAATGGGATGAAATACTAAGTGTAGTGTGTGTGAATCATTGATTCACAATTTTGTATCTGGGATCTGAAGTTTGTATTGTACTAAGAAGTGTAATGATGAATTACCTGTTAATCATTTCAGTGATTACATTTCTGCCATTGCTCTGTAATACAGTGTAGTTCAGTTGGATTGAAATTTATCCAAAATTTAGCTGATTCTGAAA

Coding sequence (CDS)

ATGGCTTCTAAAACTGTTGCTAAAGATATCATCACTCTCCGTGGCTCGGCAGCGATCGCCAGCGAATTCTTTGGGTATGCGGCAAACAGTATATTGTACAATCGAGGGGTTTACCCAGAAGAAAGTTTTGGGAAAGTGAAGAAATATGGATTGCCAATGCTTCTTACTCAAGATGAGGCTGTTAAAACTTTCATCGCTAATCTTACTGCTCAACTTTCAGAATGGCTTGAAGCAGGGAAATTACAGAGGGTTGTTTTAGTTATAATGAGCAAATCTACAAATGAAGTTCTGGAAAGATGGAATTTCAGCATTGAAACTGATAATGAAGTTGTTGAAACAGGGGTATCAAGAGAAAAAAGTGACAAGGAAATAATGAGGGAAATTCAAGCAATTATGAGGCAGATTGCTTCTAGCATCACTTACTTGCCTTGCCTTGATGAACCTTGTGTTTTTGATGTATTGGCATACACAGACAAGGATGTAGATGCACCTATTACTTGGACTGAGAGTGATCCCAAATTGATTTCTAATCCTCAAATGGTGAAACTACATTCTTTTGATACCAAGATTCACAAGGTTGACACTTTAGTTTCTTACAAGAATGATGATGATGATGATGATGAATGA

Protein sequence

MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEAVKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQMVKLHSFDTKIHKVDTLVSYKNDDDDDDE
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo05963.1Spo05963.1mRNA


Homology
[Close]
BLAST of Spo05963.1 vs. NCBI nr
Match: gi|902209205|gb|KNA16140.1| (hypothetical protein SOVF_091760 [Spinacia oleracea])

HSP 1 Score: 403.7 bits (1036), Expect = 2.000e-109
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA
Sbjct: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS
Sbjct: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDDE 209
           VKLHSFDTKIHKVDTLVSYKNDDDDDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDDE 208
[Close]
BLAST of Spo05963.1 vs. NCBI nr
Match: gi|731343402|ref|XP_010682874.1| (PREDICTED: mitotic spindle checkpoint protein MAD2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 369.8 bits (948), Expect = 3.200e-99
Identity = 188/207 (90.82%), Postives = 197/207 (95.17%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MASKTVAKDIITLRGSAAI SEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDE 
Sbjct: 1   MASKTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VK+FI NLTAQLSEWLE+GKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVE GVSREKS
Sbjct: 61  VKSFITNLTAQLSEWLESGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPC+DEPCVFDVLAYTDKD+  P TW ESDPKLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCIDEPCVFDVLAYTDKDLAVPFTWIESDPKLINNPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDD 208
           VKLH FDTKIHKVDT+VSYKND++ D+
Sbjct: 181 VKLHCFDTKIHKVDTIVSYKNDEEWDE 207
[Close]
BLAST of Spo05963.1 vs. NCBI nr
Match: gi|802724070|ref|XP_012085612.1| (PREDICTED: mitotic spindle checkpoint protein MAD2 [Jatropha curcas])

HSP 1 Score: 368.6 bits (945), Expect = 7.100e-99
Identity = 190/206 (92.23%), Postives = 195/206 (94.66%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MASKTVAKDIITLRGSAAI SEFFGYAANSILYNRGVYPEESF KVKKYGLPMLLTQDE 
Sbjct: 1   MASKTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFAKVKKYGLPMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VKTFIANLTAQLSEWLEAGKLQR+VLVIMSK TNEVLERWNFSIETD+EVVE GVSREKS
Sbjct: 61  VKTFIANLTAQLSEWLEAGKLQRIVLVIMSKVTNEVLERWNFSIETDSEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDE CVFDVLAYTDKDV  P TW ESDPKLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEACVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDD 207
           VKLHSFDTKIHKVDTLVSYKND+ D+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDEWDE 206
[Close]
BLAST of Spo05963.1 vs. NCBI nr
Match: gi|823158792|ref|XP_012479233.1| (PREDICTED: mitotic spindle checkpoint protein MAD2 [Gossypium raimondii])

HSP 1 Score: 368.6 bits (945), Expect = 7.100e-99
Identity = 188/203 (92.61%), Postives = 194/203 (95.57%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MAS+TVAKDIITLRGSAAI SEFFGYAANSILYNRGVYPEESF KVKKYGLPMLLTQDE 
Sbjct: 1   MASRTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFAKVKKYGLPMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VK+FI+NLTAQLSEWLEAGKLQRVVLVIMSK+TNEVLERWNFSIETD EVVE GVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDCEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDV  P TW ESDPKLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDD 204
           VKLHSFDTKIHKVDTLVSYKND+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDE 203
[Close]
BLAST of Spo05963.1 vs. NCBI nr
Match: gi|449435035|ref|XP_004135301.1| (PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis sativus])

HSP 1 Score: 368.2 bits (944), Expect = 9.200e-99
Identity = 188/207 (90.82%), Postives = 194/207 (93.72%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MASKT  KDIITLRGSAAI SEFFGYAANSILYNRG+YPEESF +VKKYGLPMLLTQDE 
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VKTFI+NLTAQLSEWLEAGKLQRVVLVIMSKS NEVLERWNF IETD EVVE GVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+  P TW ESDPKLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDD 208
           VKLHSFDTKIHKVDTLVSYKNDDDDD+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 207
[Close]
BLAST of Spo05963.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R9I4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_091760 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 1.400e-109
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA
Sbjct: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS
Sbjct: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDDE 209
           VKLHSFDTKIHKVDTLVSYKNDDDDDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDDE 208
[Close]
BLAST of Spo05963.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EWF2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g151570 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 2.200e-99
Identity = 188/207 (90.82%), Postives = 197/207 (95.17%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MASKTVAKDIITLRGSAAI SEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDE 
Sbjct: 1   MASKTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VK+FI NLTAQLSEWLE+GKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVE GVSREKS
Sbjct: 61  VKSFITNLTAQLSEWLESGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPC+DEPCVFDVLAYTDKD+  P TW ESDPKLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCIDEPCVFDVLAYTDKDLAVPFTWIESDPKLINNPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDD 208
           VKLH FDTKIHKVDT+VSYKND++ D+
Sbjct: 181 VKLHCFDTKIHKVDTIVSYKNDEEWDE 207
[Close]
BLAST of Spo05963.1 vs. UniProtKB/TrEMBL
Match: A0A0D2PKM1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G172500 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 4.900e-99
Identity = 188/203 (92.61%), Postives = 194/203 (95.57%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MAS+TVAKDIITLRGSAAI SEFFGYAANSILYNRGVYPEESF KVKKYGLPMLLTQDE 
Sbjct: 1   MASRTVAKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFAKVKKYGLPMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VK+FI+NLTAQLSEWLEAGKLQRVVLVIMSK+TNEVLERWNFSIETD EVVE GVSREKS
Sbjct: 61  VKSFISNLTAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDCEVVEKGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDV  P TW ESDPKLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDD 204
           VKLHSFDTKIHKVDTLVSYKND+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDE 203
[Close]
BLAST of Spo05963.1 vs. UniProtKB/TrEMBL
Match: A0A0A0KPY5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G590170 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 6.400e-99
Identity = 188/207 (90.82%), Postives = 194/207 (93.72%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MASKT  KDIITLRGSAAI SEFFGYAANSILYNRG+YPEESF +VKKYGLPMLLTQDE 
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VKTFI+NLTAQLSEWLEAGKLQRVVLVIMSKS NEVLERWNF IETD EVVE GVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+  P TW ESDPKLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDD 208
           VKLHSFDTKIHKVDTLVSYKNDDDDD+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 207
[Close]
BLAST of Spo05963.1 vs. UniProtKB/TrEMBL
Match: D7TAM3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g02430 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 1.900e-98
Identity = 187/207 (90.34%), Postives = 194/207 (93.72%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MAS+T  KDIITLRGSAAI SEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDE 
Sbjct: 1   MASRTATKDIITLRGSAAIVSEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VK+FIANLTAQLSEWLE GKLQRVVLVIMSK+TNEVLERWNFSIETD EVVE GVSREKS
Sbjct: 61  VKSFIANLTAQLSEWLETGKLQRVVLVIMSKATNEVLERWNFSIETDGEVVENGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIASSITYLPC+DEPCVFDVLAYTD DV  P TW ESDPKLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCIDEPCVFDVLAYTDTDVAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDD 208
           VKLHSFDTKIHKVDTLVSYKND+ D+D
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDEWDED 207
[Close]
BLAST of Spo05963.1 vs. ExPASy Swiss-Prot
Match: MAD2_MAIZE (Mitotic spindle checkpoint protein MAD2 OS=Zea mays GN=MAD2 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 1.700e-92
Identity = 175/208 (84.13%), Postives = 188/208 (90.38%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MAS++ +KDIITLRGSAAI SEFFGYAANSILYNR VYPEESF KVKKYGL MLLTQDE 
Sbjct: 1   MASRSASKDIITLRGSAAIVSEFFGYAANSILYNRAVYPEESFSKVKKYGLTMLLTQDEG 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           VK FIA+LT+QLSEWLEAGKLQR+VLVIMSK+T+EVLERWNF+I TD EVVE G  +EKS
Sbjct: 61  VKNFIASLTSQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFNIVTDAEVVEKGAIKEKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           DKEIMREIQAIMRQIAS ITYLPCLDEPCVFDVLAYTD DVDAP TW ESD KLI NPQM
Sbjct: 121 DKEIMREIQAIMRQIASCITYLPCLDEPCVFDVLAYTDTDVDAPGTWVESDAKLIDNPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDDE 209
           VKLHSFDTKIHKVDTLVSYK D+ D++E
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKKDEWDEEE 208
[Close]
BLAST of Spo05963.1 vs. ExPASy Swiss-Prot
Match: MAD2_ARATH (Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana GN=MAD2 PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 2.200e-92
Identity = 175/209 (83.73%), Postives = 190/209 (90.91%), Query Frame = 1 

		  

Query: 1   MASKTVA-KDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDE 60
           MASKT A KDIITL GSAAI SEFF YAANSILYNR VYPEESF KVKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  AVKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREK 120
           +VK+F++NLT+Q+SEWLEAGKLQRVVLVIMSK+T EVLERWNF IETDNEVV+ GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD DV  P TW ESDPKLI+NPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDDDDDE 209
           MVKLH FDTKIHKVDTLVSYKND+ D++E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEEE 209
[Close]
BLAST of Spo05963.1 vs. ExPASy Swiss-Prot
Match: MD2L1_DICDI (Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum GN=mad2l1-1 PE=3 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.300e-52
Identity = 106/204 (51.96%), Postives = 143/204 (70.10%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           M +   +K  I+L+GS  I +EFF Y+ N+IL+ RG+YP ESF +V KYGLP+L+T D++
Sbjct: 1   MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           +K ++ N+  QLSEWL +G +Q++VLVI    T EVLERW F + TD    E    R+K 
Sbjct: 61  LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTDIP-KEGEAPRQKP 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
           +KEIM EIQAI+RQI +S+T+LP L   C FD+L YT KD+  P  W ESDPK I+N Q 
Sbjct: 121 EKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQ 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDD 205
           VKL SF T IHKV+++V+YK  +D
Sbjct: 181 VKLRSFTTTIHKVESMVAYKISND 203
[Close]
BLAST of Spo05963.1 vs. ExPASy Swiss-Prot
Match: MD2L1_MOUSE (Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus GN=Mad2l1 PE=1 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 8.800e-49
Identity = 99/205 (48.29%), Postives = 138/205 (67.32%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MA +   +  ITLRGSA I +EFF +  NSILY RG+YP E+F +V+KYGL +L T D  
Sbjct: 1   MAQQLAREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLTTTDPE 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           +  ++ N+  QL EWL    +Q++V+VI +  + EVLERW F IE D    E GV REKS
Sbjct: 61  LIKYLNNVVEQLKEWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKEEGVRREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
            K I  EI++++RQI +++T+LP L+  C FD+L YTDKD+  P  W ES P+ I+N + 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNCEE 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDDD 206
           V+L SF T IHKV+++V+YK   +D
Sbjct: 181 VRLRSFTTTIHKVNSMVAYKTPVND 205
[Close]
BLAST of Spo05963.1 vs. ExPASy Swiss-Prot
Match: MD2L1_HUMAN (Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens GN=MAD2L1 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.800e-47
Identity = 94/200 (47.00%), Postives = 135/200 (67.50%), Query Frame = 1 

		  

Query: 1   MASKTVAKDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDEA 60
           MA +   +  ITLRGSA I +EFF +  NSILY RG+YP E+F +V+KYGL +L+T D  
Sbjct: 1   MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLE 60

Query: 61  VKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREKS 120
           +  ++ N+  QL +WL    +Q++V+VI +  + EVLERW F IE D    +    REKS
Sbjct: 61  LIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQM 180
            K I  EI++++RQI +++T+LP L+  C FD+L YTDKD+  P  W ES P+ I+N + 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEE 180

Query: 181 VKLHSFDTKIHKVDTLVSYK 201
           V+L SF T IHKV+++V+YK
Sbjct: 181 VRLRSFTTTIHKVNSMVAYK 200
[Close]
BLAST of Spo05963.1 vs. TAIR (Arabidopsis)
Match: AT3G25980.1 (DNA-binding HORMA family protein)

HSP 1 Score: 339.7 bits (870), Expect = 1.200e-93
Identity = 175/209 (83.73%), Postives = 190/209 (90.91%), Query Frame = 1 

		  

Query: 1   MASKTVA-KDIITLRGSAAIASEFFGYAANSILYNRGVYPEESFGKVKKYGLPMLLTQDE 60
           MASKT A KDIITL GSAAI SEFF YAANSILYNR VYPEESF KVKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  AVKTFIANLTAQLSEWLEAGKLQRVVLVIMSKSTNEVLERWNFSIETDNEVVETGVSREK 120
           +VK+F++NLT+Q+SEWLEAGKLQRVVLVIMSK+T EVLERWNF IETDNEVV+ GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVDAPITWTESDPKLISNPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD DV  P TW ESDPKLI+NPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDDDDDE 209
           MVKLH FDTKIHKVDTLVSYKND+ D++E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEEE 209
The following BLAST results are available for this feature:
BLAST of Spo05963.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902209205|gb|KNA16140.1|2.0e-109100.hypothetical protein SOVF_0917... [more]
gi|731343402|ref|XP_010682874.1|3.2e-9990.8PREDICTED: mitotic spindle che... [more]
gi|802724070|ref|XP_012085612.1|7.1e-9992.2PREDICTED: mitotic spindle che... [more]
gi|823158792|ref|XP_012479233.1|7.1e-9992.6PREDICTED: mitotic spindle che... [more]
gi|449435035|ref|XP_004135301.1|9.2e-9990.8PREDICTED: mitotic spindle che... [more]
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BLAST of Spo05963.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9R9I4_SPIOL1.4e-109100.Uncharacterized protein OS=Spi... [more]
A0A0J8EWF2_BETVU2.2e-9990.8Uncharacterized protein OS=Bet... [more]
A0A0D2PKM1_GOSRA4.9e-9992.6Uncharacterized protein OS=Gos... [more]
A0A0A0KPY5_CUCSA6.4e-9990.8Uncharacterized protein OS=Cuc... [more]
D7TAM3_VITVI1.9e-9890.3Putative uncharacterized prote... [more]
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BLAST of Spo05963.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
MAD2_MAIZE1.7e-9284.1Mitotic spindle checkpoint pro... [more]
MAD2_ARATH2.2e-9283.7Mitotic spindle checkpoint pro... [more]
MD2L1_DICDI1.3e-5251.9Mitotic spindle assembly check... [more]
MD2L1_MOUSE8.8e-4948.2Mitotic spindle assembly check... [more]
MD2L1_HUMAN2.8e-4747.0Mitotic spindle assembly check... [more]
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BLAST of Spo05963.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT3G25980.11.2e-9383.7DNA-binding HORMA family prote... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003511HORMA domainGENE3D3.30.900.10coord: 1..204
score: 5.8
IPR003511HORMA domainPFAMPF02301HORMAcoord: 16..191
score: 1.6
IPR003511HORMA domainPROFILEPS50815HORMAcoord: 14..197
score: 46
IPR003511HORMA domainunknownSSF56019The spindle assembly checkpoint protein mad2coord: 10..202
score: 5.49
IPR027097Mitotic spindle checkpoint protein Mad2PANTHERPTHR11842:SF11MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2Acoord: 1..208
score: 1.8E
NoneNo IPR availablePANTHERPTHR11842MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2coord: 1..208
score: 1.8E

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008283 cell proliferation
biological_process GO:0008618 7-methylguanosine metabolic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process GO:0006098 pentose-phosphate shunt
biological_process GO:0010207 photosystem II assembly
biological_process GO:0042023 DNA endoreduplication
biological_process GO:0006364 rRNA processing
biological_process GO:0006412 translation
biological_process GO:0001510 RNA methylation
biological_process GO:0006351 transcription, DNA-templated
biological_process GO:0006400 tRNA modification
biological_process GO:0051083 'de novo' cotranslational protein folding
biological_process GO:0043488 regulation of mRNA stability
biological_process GO:0006449 regulation of translational termination
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0019430 removal of superoxide radicals
biological_process GO:0008152 metabolic process
biological_process GO:0006354 DNA-templated transcription, elongation
biological_process GO:0009657 plastid organization
biological_process GO:0006566 threonine metabolic process
biological_process GO:0006544 glycine metabolic process
biological_process GO:0016572 histone phosphorylation
biological_process GO:0007094 mitotic spindle assembly checkpoint
biological_process GO:0043248 proteasome assembly
biological_process GO:0051510 regulation of unidimensional cell growth
biological_process GO:0051788 response to misfolded protein
biological_process GO:0006525 arginine metabolic process
biological_process GO:0006420 arginyl-tRNA aminoacylation
biological_process GO:0042254 ribosome biogenesis
biological_process GO:0006426 glycyl-tRNA aminoacylation
biological_process GO:0006560 proline metabolic process
biological_process GO:0006563 L-serine metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0031410 cytoplasmic vesicle
cellular_component GO:0009534 chloroplast thylakoid
cellular_component GO:0042644 chloroplast nucleoid
cellular_component GO:0018444 translation release factor complex
cellular_component GO:0009507 chloroplast
cellular_component GO:0009295 nucleoid
cellular_component GO:0000777 condensed chromosome kinetochore
cellular_component GO:0010369 chromocenter
cellular_component GO:0009345 glycine-tRNA ligase complex
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0009536 plastid
cellular_component GO:0005635 nuclear envelope
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005828 kinetochore microtubule
cellular_component GO:0016020 membrane
cellular_component GO:0005840 ribosome
molecular_function GO:0019843 rRNA binding
molecular_function GO:0003747 translation release factor activity
molecular_function GO:0003735 structural constituent of ribosome
molecular_function GO:0005524 ATP binding
molecular_function GO:0005515 protein binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004784 superoxide dismutase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004814 arginine-tRNA ligase activity
molecular_function GO:0016740 transferase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0004820 glycine-tRNA ligase activity
RNA-Seq Expression