Spo05301 (gene) | SpinachBase

Overview


Name	Spo05301
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Eukaryotic translation initiation factor 3 subunit G (eIF3g) (Eukaryotic translation initiation factor 3 RNA-binding subunit) (Eukaryotic translation initiation factor 3 subunit 4)
Location	chr6 : 14444221 .. 14448277 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTAATCCGAAACCCTTCGATTTCAAACACCCAATTCGGGTTAACACTCGACGTCTCATGAAGTCCTGAGTCCCGACTCTTGAATTCCTCACTCTTTCTATCTATCACATCCCCAAATTAGACTCCCTCTCCGAACCCTAACTTTCTCCATCCTCCCCGGCGCACTATTTCTCTCTCATCAACAACGATGCAGAACGGTAAGGTTCGATGGGGAGAACTTGACGAAGACGAAGGAGAAGATTTGGATTTCCTGCTACCTCCAAGGCAGGTGATTGGTCCAGATGACAACGGCGTCAAAAAGGTCATCGAGTATAAATTCAAAGATGATGGAACCAAGGTTAAGATCACCACCACAACTAGGGTTCGTAAGCTAGCCAAAGCTCACCTTAGCAAGAAGGCAGTTGAGCGCAGGTCTTGGTCTAAATTCGGCGATGCCGTTCACGTTGATGTCGGGGCTCGTCTTACAATGGTTTCTACTGAAGAAATAGCCCTTGAACGCCCTAAGCCTCATGGTTCGCATCGATCTCGCCATTTTTTCTATGTCGATTTTACTGGTTTTTCTGAATTTTGTTGCGAATTGTTTTGTTGCTGGATTTTGCTGTTTGATTTCGTTGTTTTTGTTGATCCTTTGAGATTGATGAATTTGGCTGTGTTGCTTTTTCTTATTGACAGTCAATTTTATGGTTAAAAGGGTGTATTTTTGCTGCATAATTGGAAGAGGGCAGGGGTTTAACTGTTTTATCGAGACTGTTCATGAGAATATCGGGGGGAATTGGAGTTTACTAGAGTTCTTTATGTATGTGTTCTTAATGTAGGACACTAATGAGGCGGCGAGTGGGTATAAATTTTGGCTCTTTATGAATTATGGTATATGTGATATCTGAAGCAATTTTGAAATTTTCTGACTGACGATCATGTAGCTGATTTGGAGATTGAAGGAATCACTACAAAAAGAAAAGAACTTGAGAAATATTTGTTTAATAATTGATGATGCTTCAGTAAGGTGCGGTTCTATTTTAATGTCGAAATAGAGTTCCGGGATAGTATTTTAGGCTGAAAGGGAAGCTTTGGATGGATTACAGTTGTTTTATTTAATCTAAAGATTTGGTGCATAGTGAATAATTTTTATTTGTTGATGGATAATTATTGGGAGGTTTTGGATAACAATAGGCTTGAGACAATAAAGACTTGAAGGTTTCACTATCTTGTTTTGTTCTGTGTCAAGTCAAAATTAAGAGATAGATTTATTGTAATTGCATTTTATAAATTTGGTTTGGTAAACATAGGTGATGGATAGATTGGTTGTCTACACGAATGATTTAACAACTTTGTGACTGGATTGTGGTGCAACTTATTATTCTTGTTGTAGTGTAAGGGAGGATTCAAGACACTTGCGTAACATTTGGATTTTAAAAAACATAGAGAATTGATTTCGTGACACGTTTTGGTGGTAAATGGAATATAAGTAGCTATTGTAGTTGGGGGAGTTGGAGAACATTATGAACACGTAGAACAATACGGTTTTTGTGGTTGTTGCTGCTGTTACTGTTTTTGTGGCCGTTTTTGTCAATCACTATTCTTCTACTGTTCTTATGTGTTGCCACTGTTATTTATTAGTAGTCTTCATATAGTGATAGTATTAGCATTGCTGTTACTAGTACTGTCAGTTTTAATATGTAACTGAATATGATTGCATTGTTCTATGTACATAGAGTTTTCAGTGATTATTTTCTTCCACAATTTTTTTTTTACAGTGTCAATATTCTTACTAATACGGGCAGGGTTGTGTATATTTGCACCTCCTCGGATCTCCCATCAACTGGATACATGATAGGTTTGGTGCTTCGTTTCAGACATGTGCTCTGATAAAAATAGAGCACTCTAGAATAGGTAGTGAGGTAATAATTGTGATAGTTATCTTAATGAACAAAGTCAAAATGGATGTTGCTCTAAATTTGACCTTCATTCGTCATACATATATGTGCACAACTGGAAAGTATGAGCTATTTCAATGGAGGAACAAGTGAAAAAGTAAGACTCTTGATGATGGAGATCATCAATAGAAAAGAATCGTGAAAGGATGGATGTCCCTCTAAGTATATCATGATAGTCTCTTATTTTATATTTGTGGTGATGTGAAACACATCAAAAATGTTTCGGTACATAGTGGCCTGTCCACATTATTTTTAATTCTTCACTTCCTCTATCATGGGCACCCGAATATCCCTACCCGAATATCTTTTCCGCTTTTGATTACATATAAAGTATTACTGGAAGAAAACAAAATATAGCTACGTTGGGATTGCTAGTTACCATCATACATTTGTTTCCATCAAATGCTTAATGTATACAGTTTATATGTTCACTGTCTCCTGATTACTCTTCTCTGTCCCTTCCCCATAAAAAAATCATACCTGACAGACTGATAAGTAGCAGTTTGCCAACAATGACAGCAAAAGATACTGGAAAATCTCATCTCTGAAATCTGGTGCTCATATATATATGTATGTTTCGAACTTCGAGTTGTGTTAAAAGACTTAAAACCTACGAAGTATTAACTAAAAAAATATATATTGGGCGTATAGTAAAAAAAATTAGCTTCGCAGAAGTAGTATAAAACCATCCCTCTTGGGGACACTCCCTCCAATGGCTGTCTCTATCGTAGGACTCTTGCTATCGCCTTTGATACCCTTGTTAGCCCTACAATGCAGTGCTGACAGTGGCAACATAGTGCAGCTAAGTATGTTAGTGGGAGTGTATTAGTAGTATCTTGGTAGTTGGATATGCGAGTATTTGAAGATTGGATGGGATCACAGGGAAGTGTCAGAATGTAGTTTAAGATTCTAGGATTTTTTGTTTTATTGAGATTCTAGGATTTTGCGTACTTTGTTTGCGGAAAGAAAGATCCTCAGAGAGTGTTGGTTGTAGCACACACCTTATACTTCTGAATGTAATTAGGCTAGACTTATATCAAGTGCTTGCTCTAGTGTTTAGAAATTAGGATGTTAGGTTTCTTGCTGTTAGTTCATCCCTTTTTTTCCCTTCTTTTTGTTCCTTTGTGAGAAGAACTGTTGACTGATGATATATTCTACGGAGTATAACTTATTTCTGATGAGTAGCAGTTTTGGATTGGAAGTGGTCCAGTGTAAATACTGGATGGTTGATTTTGTAGGACCAACTATTTTCTAGGATAATTTAATTTCTGGCATCGTAGTAAGAATTTTTTTGTTAGATCCTTGTCTATGCTGATGATCTAGTATATTCGGGAACTGTTTCTTTGTGGGTCAAGTTTTACCTGGAATTATCACAAGCCCCTATTTTAGCATTCCTTATAACGTTTGTTTTCTTGACAGGGACTAAACAAGAGGAGACTAAATCTGCAGGGGATAACCCTTTCGGTAGCAAAGGTGCTGTCCTTATGGTTTGCAGGACTTGTGGTAAAAAGGGTAACCACTGGACTGCGATGTGTCCCTACAAGGATCTCGCACAACCTCCTGAAAGTTTTGCTGATAAGCCTCCCACAGATGGGCCAGCAACTGCTTCTACAACTGGTTCCAAAGGAGCTTATGTTCCTCCGAGTTTGAGGGCAGGTGCTGAGAGAACCCCTACTGACATGAAACGCAGGAATGAAGAGAACTCAGTTCGTGTTACTAACCTATCAGAGGATACCAGAGAGGCTGATCTTCATGAGCTCTTCAGAACTTTTGGTCAGATCACCCGTGTTTATGTAGCCATTGACCAGAAGACTGGGTTGACCAGAGGTTTTGGATTTGTAAACTTTGCCATCCGAGAAGAGGCTGAGCGAGCCATCAACAAGCTTAATGGTTATGGTTATGATAATCTCATCCTTAGGGTCGAATGGGCCACGCCAAGGGCAACCTAGAATCATGAATTTTTTATAGTATATGAAATCATATTTTGATGATTCGGAAAAACAAATTACCCTTCGTCCTTTTTTATTTTTTACGTGGTAACATGCACGCGATTTAACAACTAATTCACGCGATTTAACCACTAATTAATCTTGATAAAGGAGCTCAATAATTATCACCACCA

mRNA sequence

TGGTAATCCGAAACCCTTCGATTTCAAACACCCAATTCGGGTTAACACTCGACGTCTCATGAAGTCCTGAGTCCCGACTCTTGAATTCCTCACTCTTTCTATCTATCACATCCCCAAATTAGACTCCCTCTCCGAACCCTAACTTTCTCCATCCTCCCCGGCGCACTATTTCTCTCTCATCAACAACGATGCAGAACGGTAAGGTTCGATGGGGAGAACTTGACGAAGACGAAGGAGAAGATTTGGATTTCCTGCTACCTCCAAGGCAGGTGATTGGTCCAGATGACAACGGCGTCAAAAAGGTCATCGAGTATAAATTCAAAGATGATGGAACCAAGGTTAAGATCACCACCACAACTAGGGTTCGTAAGCTAGCCAAAGCTCACCTTAGCAAGAAGGCAGTTGAGCGCAGGTCTTGGTCTAAATTCGGCGATGCCGTTCACGTTGATGTCGGGGCTCGTCTTACAATGGTTTCTACTGAAGAAATAGCCCTTGAACGCCCTAAGCCTCATGGGACTAAACAAGAGGAGACTAAATCTGCAGGGGATAACCCTTTCGGTAGCAAAGGTGCTGTCCTTATGGTTTGCAGGACTTGTGGTAAAAAGGGTAACCACTGGACTGCGATGTGTCCCTACAAGGATCTCGCACAACCTCCTGAAAGTTTTGCTGATAAGCCTCCCACAGATGGGCCAGCAACTGCTTCTACAACTGGTTCCAAAGGAGCTTATGTTCCTCCGAGTTTGAGGGCAGGTGCTGAGAGAACCCCTACTGACATGAAACGCAGGAATGAAGAGAACTCAGTTCGTGTTACTAACCTATCAGAGGATACCAGAGAGGCTGATCTTCATGAGCTCTTCAGAACTTTTGGTCAGATCACCCGTGTTTATGTAGCCATTGACCAGAAGACTGGGTTGACCAGAGGTTTTGGATTTGTAAACTTTGCCATCCGAGAAGAGGCTGAGCGAGCCATCAACAAGCTTAATGGTTATGGTTATGATAATCTCATCCTTAGGGTCGAATGGGCCACGCCAAGGGCAACCTAGAATCATGAATTTTTTATAGTATATGAAATCATATTTTGATGATTCGGAAAAACAAATTACCCTTCGTCCTTTTTTATTTTTTACGTGGTAACATGCACGCGATTTAACAACTAATTCACGCGATTTAACCACTAATTAATCTTGATAAAGGAGCTCAATAATTATCACCACCA

Coding sequence (CDS)

ATGCAGAACGGTAAGGTTCGATGGGGAGAACTTGACGAAGACGAAGGAGAAGATTTGGATTTCCTGCTACCTCCAAGGCAGGTGATTGGTCCAGATGACAACGGCGTCAAAAAGGTCATCGAGTATAAATTCAAAGATGATGGAACCAAGGTTAAGATCACCACCACAACTAGGGTTCGTAAGCTAGCCAAAGCTCACCTTAGCAAGAAGGCAGTTGAGCGCAGGTCTTGGTCTAAATTCGGCGATGCCGTTCACGTTGATGTCGGGGCTCGTCTTACAATGGTTTCTACTGAAGAAATAGCCCTTGAACGCCCTAAGCCTCATGGGACTAAACAAGAGGAGACTAAATCTGCAGGGGATAACCCTTTCGGTAGCAAAGGTGCTGTCCTTATGGTTTGCAGGACTTGTGGTAAAAAGGGTAACCACTGGACTGCGATGTGTCCCTACAAGGATCTCGCACAACCTCCTGAAAGTTTTGCTGATAAGCCTCCCACAGATGGGCCAGCAACTGCTTCTACAACTGGTTCCAAAGGAGCTTATGTTCCTCCGAGTTTGAGGGCAGGTGCTGAGAGAACCCCTACTGACATGAAACGCAGGAATGAAGAGAACTCAGTTCGTGTTACTAACCTATCAGAGGATACCAGAGAGGCTGATCTTCATGAGCTCTTCAGAACTTTTGGTCAGATCACCCGTGTTTATGTAGCCATTGACCAGAAGACTGGGTTGACCAGAGGTTTTGGATTTGTAAACTTTGCCATCCGAGAAGAGGCTGAGCGAGCCATCAACAAGCTTAATGGTTATGGTTATGATAATCTCATCCTTAGGGTCGAATGGGCCACGCCAAGGGCAACCTAG

Protein sequence

MQNGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGDNPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAYVPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo05301.1	Spo05301.1	mRNA

Homology

[Close]

BLAST of Spo05301.1 vs. NCBI nr
Match: gi|902094662|gb|KNA03373.1| (hypothetical protein SOVF_209820 [Spinacia oleracea])

HSP 1 Score: 575.5 bits (1482), Expect = 5.200e-161
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 1


		  
Query: 1   MQNGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVR 60
           MQNGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVR
Sbjct: 1   MQNGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVR 60

Query: 61  KLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGD 120
           KLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGD
Sbjct: 61  KLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGD 120

Query: 121 NPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAY 180
           NPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAY
Sbjct: 121 NPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAY 180

Query: 181 VPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKT 240
           VPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKT
Sbjct: 181 VPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKT 240

Query: 241 GLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT 285
           GLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 GLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT 284

[Close]

BLAST of Spo05301.1 vs. NCBI nr
Match: gi|902221241|gb|KNA18844.1| (hypothetical protein SOVF_067190 [Spinacia oleracea])

HSP 1 Score: 559.3 bits (1440), Expect = 3.900e-156
Identity = 277/287 (96.52%), Postives = 281/287 (97.91%), Query Frame = 1


		  
Query: 1   MQNG---KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTT 60
           MQNG   KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDG+KVKITTTT
Sbjct: 1   MQNGGQNKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGSKVKITTTT 60

Query: 61  RVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKS 120
           RVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHG KQEETKS
Sbjct: 61  RVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGAKQEETKS 120

Query: 121 AGDNPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSK 180
           AGDNPFGSKGA+LM+CRTCGKKGNHWTAMCPYKDLA PPESFADKPPTDGPA ASTTGSK
Sbjct: 121 AGDNPFGSKGAMLMICRTCGKKGNHWTAMCPYKDLAPPPESFADKPPTDGPAAASTTGSK 180

Query: 181 GAYVPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAID 240
           GAYVPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAID
Sbjct: 181 GAYVPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAID 240

Query: 241 QKTGLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT 285
           QKTGL+RGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 QKTGLSRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT 287

[Close]

BLAST of Spo05301.1 vs. NCBI nr
Match: gi|731348810|ref|XP_010685677.1| (PREDICTED: eukaryotic translation initiation factor 3 subunit G-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 497.7 bits (1280), Expect = 1.400e-137
Identity = 243/280 (86.79%), Postives = 264/280 (94.29%), Query Frame = 1


		  
Query: 4   GKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLA 63
           GK+RWGELDEDEGEDLDFLLPP+QVIGPD+NG+KKVIEYKFKDDG KVKITTTTRVRKLA
Sbjct: 9   GKLRWGELDEDEGEDLDFLLPPKQVIGPDENGIKKVIEYKFKDDGNKVKITTTTRVRKLA 68

Query: 64  KAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGDNPF 123
           KAHLSKKA+ERRSWSKFGDAV+ DVGARLT+VSTEEI LERP   GTK+E+ KS+GDNPF
Sbjct: 69  KAHLSKKAIERRSWSKFGDAVNEDVGARLTVVSTEEICLERPTAPGTKEEDKKSSGDNPF 128

Query: 124 GSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAYVPP 183
           G  GA LM+CRTCGKKG+HWT+ CPYKDLAQPPE+F+DKP TDGPA A+TT +KGAYVPP
Sbjct: 129 GKAGATLMLCRTCGKKGDHWTSRCPYKDLAQPPETFSDKPATDGPA-AATTATKGAYVPP 188

Query: 184 SLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGLT 243
           SLRAGAERT TDM+RRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGL+
Sbjct: 189 SLRAGAERTVTDMRRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGLS 248

Query: 244 RGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRA 284
           RGFGFVNFA RE+AERAINKLNGYGYDNLILRVEWATPR+
Sbjct: 249 RGFGFVNFANREDAERAINKLNGYGYDNLILRVEWATPRS 287

[Close]

BLAST of Spo05301.1 vs. NCBI nr
Match: gi|224109462|ref|XP_002315204.1| (eukaryotic translation initiation factor 3G family protein [Populus trichocarpa])

HSP 1 Score: 455.7 bits (1171), Expect = 6.000e-125
Identity = 224/281 (79.72%), Postives = 251/281 (89.32%), Query Frame = 1


		  
Query: 5   KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLAK 64
           K+RWGELDE++GEDLDFLLPP+QVIGPD+NG+KKVIEYKF DDG KVKITTTTRVRKLAK
Sbjct: 12  KMRWGELDEEDGEDLDFLLPPKQVIGPDENGIKKVIEYKFNDDGNKVKITTTTRVRKLAK 71

Query: 65  AHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGDN--P 124
           A LSK+A+ERR+WSKFGDA+H DVG+RLTMVSTEEI LERP+ HG K +ETK AGDN   
Sbjct: 72  ARLSKRALERRNWSKFGDAIHEDVGSRLTMVSTEEILLERPRAHGAKADETKIAGDNLAQ 131

Query: 125 FGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAYVP 184
            G  GAVLMVCRTCGKKG+HWT+ CPYKDLAQPPE+F DKPP    A A+T  +KGAYVP
Sbjct: 132 LGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAQPPETFIDKPPATETAMAATGATKGAYVP 191

Query: 185 PSLRAGAERT-PTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTG 244
           PS+RAGAERT  +DM+RRNEENSVRVTNLSEDTRE DL ELFRTFGQ++RVYVAIDQKTG
Sbjct: 192 PSMRAGAERTVGSDMRRRNEENSVRVTNLSEDTREPDLLELFRTFGQVSRVYVAIDQKTG 251

Query: 245 LTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPR 283
           ++RGFGFVNF  +E+AERAINKLNGYGYDNLILRVEWATPR
Sbjct: 252 VSRGFGFVNFVSKEDAERAINKLNGYGYDNLILRVEWATPR 292

[Close]

BLAST of Spo05301.1 vs. NCBI nr
Match: gi|702424313|ref|XP_010067450.1| (PREDICTED: eukaryotic translation initiation factor 3 subunit G-like [Eucalyptus grandis])

HSP 1 Score: 452.6 bits (1163), Expect = 5.100e-124
Identity = 227/281 (80.78%), Postives = 247/281 (87.90%), Query Frame = 1


		  
Query: 5   KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLAK 64
           K+RWGELDED+GEDLDFLLPPRQVIGPD+NG+KKVIEYKF DDG KVKITTTTRVRKLAK
Sbjct: 16  KLRWGELDEDDGEDLDFLLPPRQVIGPDENGIKKVIEYKFNDDGNKVKITTTTRVRKLAK 75

Query: 65  AHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGDN--P 124
           A LSK AVERR+W+KFGDAVH DVGARLTMVSTEEI LERP+  GTK EE+K AGDN   
Sbjct: 76  ARLSKSAVERRNWAKFGDAVHEDVGARLTMVSTEEILLERPRAPGTKGEESKVAGDNLAQ 135

Query: 125 FGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAYVP 184
            G  GAVLMVCRTCGKKG+HWT+ CPYKDLAQP E+F DK     PA A  T +KGAYVP
Sbjct: 136 LGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAQPVETFVDK-----PAAAEPTSTKGAYVP 195

Query: 185 PSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGL 244
           PS+RAGAERT T+M+RRNEENSVRVTNLSEDTRE DL ELFRTFG ++RVYVAIDQKTGL
Sbjct: 196 PSMRAGAERTGTEMRRRNEENSVRVTNLSEDTREPDLLELFRTFGPVSRVYVAIDQKTGL 255

Query: 245 TRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRA 284
           +RGFGFVNF  +E+AERAINKLNGYGYDNLILRVEWATPRA
Sbjct: 256 SRGFGFVNFVNKEDAERAINKLNGYGYDNLILRVEWATPRA 291

[Close]

BLAST of Spo05301.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9K4_SPIOL (Eukaryotic translation initiation factor 3 subunit G OS=Spinacia oleracea GN=SOVF_209820 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 3.600e-161
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 1


		  
Query: 1   MQNGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVR 60
           MQNGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVR
Sbjct: 1   MQNGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVR 60

Query: 61  KLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGD 120
           KLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGD
Sbjct: 61  KLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGD 120

Query: 121 NPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAY 180
           NPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAY
Sbjct: 121 NPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAY 180

Query: 181 VPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKT 240
           VPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKT
Sbjct: 181 VPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKT 240

Query: 241 GLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT 285
           GLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 GLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT 284

[Close]

BLAST of Spo05301.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RIT4_SPIOL (Eukaryotic translation initiation factor 3 subunit G OS=Spinacia oleracea GN=SOVF_067190 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 2.700e-156
Identity = 277/287 (96.52%), Postives = 281/287 (97.91%), Query Frame = 1


		  
Query: 1   MQNG---KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTT 60
           MQNG   KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDG+KVKITTTT
Sbjct: 1   MQNGGQNKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGSKVKITTTT 60

Query: 61  RVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKS 120
           RVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHG KQEETKS
Sbjct: 61  RVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGAKQEETKS 120

Query: 121 AGDNPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSK 180
           AGDNPFGSKGA+LM+CRTCGKKGNHWTAMCPYKDLA PPESFADKPPTDGPA ASTTGSK
Sbjct: 121 AGDNPFGSKGAMLMICRTCGKKGNHWTAMCPYKDLAPPPESFADKPPTDGPAAASTTGSK 180

Query: 181 GAYVPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAID 240
           GAYVPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAID
Sbjct: 181 GAYVPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAID 240

Query: 241 QKTGLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT 285
           QKTGL+RGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT
Sbjct: 241 QKTGLSRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRAT 287

[Close]

BLAST of Spo05301.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BVM2_BETVU (Eukaryotic translation initiation factor 3 subunit G OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g173200 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 9.600e-138
Identity = 243/280 (86.79%), Postives = 264/280 (94.29%), Query Frame = 1


		  
Query: 4   GKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLA 63
           GK+RWGELDEDEGEDLDFLLPP+QVIGPD+NG+KKVIEYKFKDDG KVKITTTTRVRKLA
Sbjct: 9   GKLRWGELDEDEGEDLDFLLPPKQVIGPDENGIKKVIEYKFKDDGNKVKITTTTRVRKLA 68

Query: 64  KAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGDNPF 123
           KAHLSKKA+ERRSWSKFGDAV+ DVGARLT+VSTEEI LERP   GTK+E+ KS+GDNPF
Sbjct: 69  KAHLSKKAIERRSWSKFGDAVNEDVGARLTVVSTEEICLERPTAPGTKEEDKKSSGDNPF 128

Query: 124 GSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAYVPP 183
           G  GA LM+CRTCGKKG+HWT+ CPYKDLAQPPE+F+DKP TDGPA A+TT +KGAYVPP
Sbjct: 129 GKAGATLMLCRTCGKKGDHWTSRCPYKDLAQPPETFSDKPATDGPA-AATTATKGAYVPP 188

Query: 184 SLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGLT 243
           SLRAGAERT TDM+RRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGL+
Sbjct: 189 SLRAGAERTVTDMRRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGLS 248

Query: 244 RGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRA 284
           RGFGFVNFA RE+AERAINKLNGYGYDNLILRVEWATPR+
Sbjct: 249 RGFGFVNFANREDAERAINKLNGYGYDNLILRVEWATPRS 287

[Close]

BLAST of Spo05301.1 vs. UniProtKB/TrEMBL
Match: B9HT66_POPTR (Eukaryotic translation initiation factor 3 subunit G OS=Populus trichocarpa GN=POPTR_0010s20650g PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 4.200e-125
Identity = 224/281 (79.72%), Postives = 251/281 (89.32%), Query Frame = 1


		  
Query: 5   KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLAK 64
           K+RWGELDE++GEDLDFLLPP+QVIGPD+NG+KKVIEYKF DDG KVKITTTTRVRKLAK
Sbjct: 12  KMRWGELDEEDGEDLDFLLPPKQVIGPDENGIKKVIEYKFNDDGNKVKITTTTRVRKLAK 71

Query: 65  AHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGDN--P 124
           A LSK+A+ERR+WSKFGDA+H DVG+RLTMVSTEEI LERP+ HG K +ETK AGDN   
Sbjct: 72  ARLSKRALERRNWSKFGDAIHEDVGSRLTMVSTEEILLERPRAHGAKADETKIAGDNLAQ 131

Query: 125 FGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAYVP 184
            G  GAVLMVCRTCGKKG+HWT+ CPYKDLAQPPE+F DKPP    A A+T  +KGAYVP
Sbjct: 132 LGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAQPPETFIDKPPATETAMAATGATKGAYVP 191

Query: 185 PSLRAGAERT-PTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTG 244
           PS+RAGAERT  +DM+RRNEENSVRVTNLSEDTRE DL ELFRTFGQ++RVYVAIDQKTG
Sbjct: 192 PSMRAGAERTVGSDMRRRNEENSVRVTNLSEDTREPDLLELFRTFGQVSRVYVAIDQKTG 251

Query: 245 LTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPR 283
           ++RGFGFVNF  +E+AERAINKLNGYGYDNLILRVEWATPR
Sbjct: 252 VSRGFGFVNFVSKEDAERAINKLNGYGYDNLILRVEWATPR 292

[Close]

BLAST of Spo05301.1 vs. UniProtKB/TrEMBL
Match: A0A059BHS4_EUCGR (Eukaryotic translation initiation factor 3 subunit G OS=Eucalyptus grandis GN=EUGRSUZ_G02987 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 3.500e-124
Identity = 227/281 (80.78%), Postives = 247/281 (87.90%), Query Frame = 1


		  
Query: 5   KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLAK 64
           K+RWGELDED+GEDLDFLLPPRQVIGPD+NG+KKVIEYKF DDG KVKITTTTRVRKLAK
Sbjct: 16  KLRWGELDEDDGEDLDFLLPPRQVIGPDENGIKKVIEYKFNDDGNKVKITTTTRVRKLAK 75

Query: 65  AHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGDN--P 124
           A LSK AVERR+W+KFGDAVH DVGARLTMVSTEEI LERP+  GTK EE+K AGDN   
Sbjct: 76  ARLSKSAVERRNWAKFGDAVHEDVGARLTMVSTEEILLERPRAPGTKGEESKVAGDNLAQ 135

Query: 125 FGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGSKGAYVP 184
            G  GAVLMVCRTCGKKG+HWT+ CPYKDLAQP E+F DK     PA A  T +KGAYVP
Sbjct: 136 LGKGGAVLMVCRTCGKKGDHWTSRCPYKDLAQPVETFVDK-----PAAAEPTSTKGAYVP 195

Query: 185 PSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGL 244
           PS+RAGAERT T+M+RRNEENSVRVTNLSEDTRE DL ELFRTFG ++RVYVAIDQKTGL
Sbjct: 196 PSMRAGAERTGTEMRRRNEENSVRVTNLSEDTREPDLLELFRTFGPVSRVYVAIDQKTGL 255

Query: 245 TRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRA 284
           +RGFGFVNF  +E+AERAINKLNGYGYDNLILRVEWATPRA
Sbjct: 256 SRGFGFVNFVNKEDAERAINKLNGYGYDNLILRVEWATPRA 291

[Close]

BLAST of Spo05301.1 vs. ExPASy Swiss-Prot
Match: EI3GB_XENLA (Eukaryotic translation initiation factor 3 subunit G-B OS=Xenopus laevis GN=eif3g-b PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 8.000e-45
Identity = 120/309 (38.83%), Postives = 167/309 (54.05%), Query Frame = 1


		  
Query: 3   NGKVRWGELDEDEGEDLDFLLP--------------PRQVIGPDDNGVKKVIEYKFKDDG 62
           + K  W +  E+EG D++ L P              PR+VI   +  +K + EYK  D  
Sbjct: 7   DSKPSWADQVEEEGIDVEPLSPQIKKQDPASFVLDTPREVI---NGNIKTITEYKLNDKD 66

Query: 63  TKVKITTTTRVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPH 122
            K+KI  T ++  L     SK    R++W KFG++ +   G  +   +  +  L     +
Sbjct: 67  KKIKIVRTFKIETL---KASKVVAHRKNWKKFGNSEYDPPGPNVATTTVSDDVLMTFITN 126

Query: 123 GTKQEETKSAGDNPFGS-KGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPE---------S 182
             K++      ++P    KG  ++ CR C  KG+HWT  CPYKD   P +         S
Sbjct: 127 --KEDLNNQEEEDPMNKLKGQKIVSCRIC--KGDHWTTRCPYKDTLGPMQKELAEQLGLS 186

Query: 183 FADKPPTDG--PATASTTGSK-GAYVPPSLRAGAERTPTDMK--RRNEEN-SVRVTNLSE 242
            ADK    G  P  A    SK G YVPPSLR G  R    M+  RR ++N ++RVTNLSE
Sbjct: 187 TADKEKAPGAEPEPAQAPVSKTGKYVPPSLRDGGSRRGESMQPNRRADDNATIRVTNLSE 246

Query: 243 DTREADLHELFRTFGQITRVYVAIDQKTGLTRGFGFVNFAIREEAERAINKLNGYGYDNL 282
           DTRE DL ELFR FG I+R+Y+A D+ TG ++GF F++F  RE+A RAI  ++G+GYD+L
Sbjct: 247 DTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHL 305

[Close]

BLAST of Spo05301.1 vs. ExPASy Swiss-Prot
Match: EIF3G_XENTR (Eukaryotic translation initiation factor 3 subunit G OS=Xenopus tropicalis GN=eif3g PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.300e-44
Identity = 119/308 (38.64%), Postives = 166/308 (53.90%), Query Frame = 1


		  
Query: 3   NGKVRWGELDEDEGEDLDFLLPPRQVIGPDD------------NG-VKKVIEYKFKDDGT 62
           + K  W +  E+EG D + + PP   +  D             NG +K + EYK  D+G 
Sbjct: 7   DSKPSWADQVEEEGIDPEPISPPVTKVQQDPASFVLDTPQEVINGKIKTITEYKLNDEGK 66

Query: 63  KVKITTTTRVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHG 122
           K+KI  T ++  L     SK    R++W KFG++ +   G  +   +  +  L       
Sbjct: 67  KIKIVRTFKIETLKA---SKVVAHRKNWKKFGNSEYDPPGPNVATTTVSDDVLMTFIT-- 126

Query: 123 TKQEETKSAGDNPFGS-KGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPE---------SF 182
           +K++      ++P    KG  ++ CR C  KG+HWT  CPYKD   P +         S 
Sbjct: 127 SKEDLNNQEEEDPMNKLKGQKIVSCRIC--KGDHWTTRCPYKDTLGPMQKELAEQLGLST 186

Query: 183 ADKPPTDG--PATASTTGSK-GAYVPPSLRAGAERTPTDMK--RRNEENS-VRVTNLSED 242
            DK    G  P  A    SK G YVPPSLR G  R    M+  RR ++N+ +RVTNLSED
Sbjct: 187 GDKEKAPGAEPEPAQAPVSKTGKYVPPSLRDGGSRRGESMQPNRRADDNATIRVTNLSED 246

Query: 243 TREADLHELFRTFGQITRVYVAIDQKTGLTRGFGFVNFAIREEAERAINKLNGYGYDNLI 282
           TRE DL ELFR FG I+R+Y+A D+ TG ++GF F++F  RE+A RAI  ++G+GYD+LI
Sbjct: 247 TRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLI 306

[Close]

BLAST of Spo05301.1 vs. ExPASy Swiss-Prot
Match: EI3GA_XENLA (Eukaryotic translation initiation factor 3 subunit G-A OS=Xenopus laevis GN=eif3g-a PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.100e-44
Identity = 120/309 (38.83%), Postives = 165/309 (53.40%), Query Frame = 1


		  
Query: 3   NGKVRWGELDEDEGEDLDFLLP--------------PRQVIGPDDNGVKKVIEYKFKDDG 62
           + K  W +  E+EG D + L P              PR+VI   +  +K + EYK  DD 
Sbjct: 7   DSKPSWADQVEEEGIDAEPLSPQIRKPDPVSFVLDTPREVI---NGNIKTITEYKLNDDD 66

Query: 63  TKVKITTTTRVRKLAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPH 122
             VKI    R  K+     SK   +R++W KFG++ +   G  +   +  +  L     +
Sbjct: 67  KTVKI---VRTFKIETVKASKVVAQRKNWKKFGNSEYDPPGPNVATTTVSDDVLMTFITN 126

Query: 123 GTKQEETKSAGDNPFGS-KGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPE---------S 182
             K++      ++P    KG  ++ CR C  KG+HWT  CPYKD   P +         S
Sbjct: 127 --KEDLNNQEEEDPMNKLKGQKIVSCRIC--KGDHWTTRCPYKDTLGPMQKELAEQLGLS 186

Query: 183 FADKPPTDG--PATASTTGSK-GAYVPPSLRAGAERTPTDMK--RRNEEN-SVRVTNLSE 242
             DK    G  P  A    SK G YVPPSLR G  R    M+  RR ++N ++RVTNLSE
Sbjct: 187 TGDKEKAPGAEPEPAQAPVSKTGKYVPPSLRDGGSRRGESMQPNRRADDNATIRVTNLSE 246

Query: 243 DTREADLHELFRTFGQITRVYVAIDQKTGLTRGFGFVNFAIREEAERAINKLNGYGYDNL 282
           DTRE DL ELFR FG I+R+Y+A D+ TG ++GF F++F  RE+A RAI  ++G+GYD+L
Sbjct: 247 DTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHL 305

[Close]

BLAST of Spo05301.1 vs. ExPASy Swiss-Prot
Match: EIF3G_DANRE (Eukaryotic translation initiation factor 3 subunit G OS=Danio rerio GN=eif3g PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.000e-43
Identity = 117/295 (39.66%), Postives = 165/295 (55.93%), Query Frame = 1


		  
Query: 3   NGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKL 62
           + K  W +  E+EG++     P   V G     +K V EYK  DDG K KI  T ++ + 
Sbjct: 8   DSKPSWADQVEEEGDEGSLPSPKETVKG----NIKTVTEYKIDDDGQKFKIIRTFKI-ET 67

Query: 63  AKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEE--TKSAGD 122
            KA  SK    R++W KFG++ +   G     V+T  ++ +      + +E+   +   +
Sbjct: 68  RKA--SKAVARRKNWKKFGNSEYDAPGPN---VATTTVSDDVFMTFISSKEDLNAQDQDE 127

Query: 123 NPFGS-KGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPE---------SFADKPPTDGPAT 182
           +P    KG  ++ CR C  KG+HWT  CPYKD   P +         S  +K     P  
Sbjct: 128 DPMNKLKGQKIVSCRIC--KGDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKAAEPEP 187

Query: 183 ASTTGSK-GAYVPPSLRAGAERTPTDMK--RRNEENS-VRVTNLSEDTREADLHELFRTF 242
           A    +K G YVPPSLR G  R    M+  RR ++N+ +RVTNLSEDTRE DL ELFR F
Sbjct: 188 AQPVQNKTGKYVPPSLRDGGTRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPF 247

Query: 243 GQITRVYVAIDQKTGLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATP 282
           G I+R+Y+A D+ TG ++GF F++F  RE+A RAI  ++G+GYD+LIL VEWA P
Sbjct: 248 GSISRIYLAKDKNTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKP 290

[Close]

BLAST of Spo05301.1 vs. ExPASy Swiss-Prot
Match: EIF3G_HUMAN (Eukaryotic translation initiation factor 3 subunit G OS=Homo sapiens GN=EIF3G PE=1 SV=2)

HSP 1 Score: 177.2 bits (448), Expect = 2.600e-43
Identity = 114/276 (41.30%), Postives = 158/276 (57.25%), Query Frame = 1


		  
Query: 22  LLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLAKAHLSKKAVERRSWSKFG 81
           L PP++VI   +  +K V EYK  +DG K KI  T R+ +  KA  SK    R++W KFG
Sbjct: 53  LPPPKEVI---NGNIKTVTEYKIDEDGKKFKIVRTFRI-ETRKA--SKAVARRKNWKKFG 112

Query: 82  DAVHVDVGARLTMVSTEEIALERPKPHGTKQEETK-SAGDNPFGS-KGAVLMVCRTCGKK 141
           ++     G     V+T  ++ +      T +E+      ++P    KG  ++ CR C  K
Sbjct: 113 NSEFDPPGPN---VATTTVSDDVSMTFITSKEDLNCQEEEDPMNKLKGQKIVSCRIC--K 172

Query: 142 GNHWTAMCPYKDLAQPPESFA-----------DKPPTDGPATASTTGSKGAYVPPSLRAG 201
           G+HWT  CPYKD   P +              +K P +     +T    G YVPPSLR G
Sbjct: 173 GDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKLPGELEPVQATQNKTGKYVPPSLRDG 232

Query: 202 AERTPTDMK--RRNEENS-VRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGLTRG 261
           A R    M+  RR ++N+ +RVTNLSEDTRE DL ELFR FG I+R+Y+A D+ TG ++G
Sbjct: 233 ASRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTTGQSKG 292

Query: 262 FGFVNFAIREEAERAINKLNGYGYDNLILRVEWATP 282
           F F++F  RE+A RAI  ++G+GYD+LIL VEWA P
Sbjct: 293 FAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKP 317

[Close]

BLAST of Spo05301.1 vs. TAIR (Arabidopsis)
Match: AT3G11400.2 (eukaryotic translation initiation factor 3G1)

HSP 1 Score: 375.6 bits (963), Expect = 2.800e-104
Identity = 198/316 (62.66%), Postives = 231/316 (73.10%), Query Frame = 1


		  
Query: 2   QNGKVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRK 61
           +  K RWGE+DEDE  DLDFLLPP+QVIGPD+NG+K  IEYKF D+  KVKITT TRVRK
Sbjct: 8   KTSKFRWGEMDEDE--DLDFLLPPKQVIGPDENGLKTTIEYKFNDEENKVKITTRTRVRK 67

Query: 62  LAKAHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHG------------ 121
           LA A L+K+A+ERR+W KFGDA + + G+ LTMVSTEEI LERP+  G            
Sbjct: 68  LASARLNKRAMERRNWPKFGDAANEEAGSHLTMVSTEEILLERPRAPGDQLANHMLASIP 127

Query: 122 ---------------TKQEETKSAGD--NPFGSKGAVLMVCRTCGKKGNHWTAMCPYKDL 181
                          TK +E+K+ GD  +  G  GAVLMVCR C KKG+HWT+ CPYKDL
Sbjct: 128 EYELKCALTFVAANCTKADESKATGDGLSQLGKGGAVLMVCRICHKKGDHWTSKCPYKDL 187

Query: 182 AQPPESFADKPPTDGPATASTTGSKG--AYVPPSLRAGAERTP--TDMKRRNEENSVRVT 241
           A P + F DKPPT   +T S     G  AYVPPS+RAGA+R+   +DM+RRN+ENSVRVT
Sbjct: 188 AAPTDVFIDKPPTGESSTMSAAPGTGKAAYVPPSMRAGADRSAVGSDMRRRNDENSVRVT 247

Query: 242 NLSEDTREADLHELFRTFGQITRVYVAIDQKTGLTRGFGFVNFAIREEAERAINKLNGYG 285
           NLSEDTRE DL ELF  FG +TRVYVAIDQKTG++RGFGFVNF  RE+A+RAINKLNGYG
Sbjct: 248 NLSEDTREPDLMELFHPFGAVTRVYVAIDQKTGVSRGFGFVNFVSREDAQRAINKLNGYG 307

[Close]

BLAST of Spo05301.1 vs. TAIR (Arabidopsis)
Match: AT5G06000.1 (eukaryotic translation initiation factor 3G2)

HSP 1 Score: 334.0 bits (855), Expect = 9.300e-92
Identity = 176/283 (62.19%), Postives = 219/283 (77.39%), Query Frame = 1


		  
Query: 5   KVRWGELDEDEGEDLDFLLPPRQVIGPDDNGVKKVIEYKFKDDGTKVKITTTTRVRKLAK 64
           K+RWGE+DE+EG D DFLLPP+Q+I PD NGVKKVIEYKF ++  KVKITTTTRV+K A 
Sbjct: 11  KLRWGEIDEEEG-DYDFLLPPKQMISPDQNGVKKVIEYKFNEEDKKVKITTTTRVQKRA- 70

Query: 65  AHLSKKAVERRSWSKFGDAVHVDVGARLTMVSTEEIALERPKPHGTKQEETKSAGDN--P 124
             L+K+AVERRSW+KFGDA H +  + LTM STE+I LER +  G+  E++  +GD+   
Sbjct: 71  --LTKQAVERRSWNKFGDAAHEESSSYLTMRSTEDIILERIRAPGSNAEQSTVSGDSMSQ 130

Query: 125 FGSKGAVLMVCRTCGKKGNHWTAMCPYKDLAQPPESFADKPPTDGPATASTTGS-KGAYV 184
            G  GAVLMVCR C KKG+HWT+ CP KDL     S  D+P T   +T++ TG+ + AYV
Sbjct: 131 LGKPGAVLMVCRLCQKKGDHWTSRCPQKDLL----SLMDEPLTAETSTSTITGTGRAAYV 190

Query: 185 PPSLRAGAERTP--TDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQK 244
           PPS+R GA+R    +DM+ R++ENSVRVTNLSEDTR  DL ELFR FG +TR +VAIDQK
Sbjct: 191 PPSMREGADRKAGGSDMRSRHDENSVRVTNLSEDTRGPDLMELFRPFGAVTRCHVAIDQK 250

Query: 245 TGLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPR 283
           T ++RGFGFV+F  RE+A+RAINKLNGYGYDNLILRVEW+TP+
Sbjct: 251 TSMSRGFGFVSFVSREDAQRAINKLNGYGYDNLILRVEWSTPK 285

[Close]

BLAST of Spo05301.1 vs. TAIR (Arabidopsis)
Match: AT3G14100.1 (RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 66.2 bits (160), Expect = 3.600e-11
Identity = 32/81 (39.51%), Postives = 50/81 (61.73%), Query Frame = 1


		  
Query: 204 SVRVTNLSEDTREADLHELFRTFGQITRVYVAIDQKTGLTRGFGFVNFAIREEAERAINK 263
           ++ V +LS +  +A L++ F  F   +   V  DQKTG +RGFGFV+F  +++A+ AIN+
Sbjct: 145 NIFVGDLSPEVTDATLYQSFSVFSSCSDARVMWDQKTGRSRGFGFVSFRNQQDAQTAINE 204

Query: 264 LNGYGYDNLILRVEWATPRAT 285
           +NG    +  +R  WAT  AT
Sbjct: 205 MNGKWLSSRQIRCNWATKGAT 225

[Close]

BLAST of Spo05301.1 vs. TAIR (Arabidopsis)
Match: AT1G54080.2 (oligouridylate-binding protein 1A)

HSP 1 Score: 62.4 bits (150), Expect = 5.200e-10
Identity = 33/85 (38.82%), Postives = 50/85 (58.82%), Query Frame = 1


		  
Query: 204 SVRVTNLSEDTREADLHELFRTFGQITRVY----VAIDQKTGLTRGFGFVNFAIREEAER 263
           ++ V +LS +  +A L + F  F   +  Y    V  DQKTG +RGFGFV+F  +++A+ 
Sbjct: 149 NIFVGDLSPEVTDAALFDSFSAFNSCSSYYRDARVMWDQKTGRSRGFGFVSFRNQQDAQT 208

Query: 264 AINKLNGYGYDNLILRVEWATPRAT 285
           AIN++NG    +  +R  WAT  AT
Sbjct: 209 AINEMNGKWVSSRQIRCNWATKGAT 233

[Close]

BLAST of Spo05301.1 vs. TAIR (Arabidopsis)
Match: AT4G34110.1 (poly(A) binding protein 2)

HSP 1 Score: 62.0 bits (149), Expect = 6.800e-10
Identity = 43/107 (40.19%), Postives = 58/107 (54.21%), Query Frame = 1


		  
Query: 177 KGAYVPPSLRAGAERTPTDMKRRNEENSVRVTNLSEDTREADLHELFRTFGQITRVYVAI 236
           K  YV P LR   ER  T  K +    +V V NL+E T + DL   F  +G+IT   V  
Sbjct: 192 KQVYVGPFLRR-QERDSTANKTKF--TNVYVKNLAESTTDDDLKNAFGEYGKITSAVVMK 251

Query: 237 DQKTGLTRGFGFVNFAIREEAERAINKLNGYGYDNLILRVEWATPRA 284
           D + G ++GFGFVNF   ++A RA+  LNG+ +D+     EW   RA
Sbjct: 252 DGE-GKSKGFGFVNFENADDAARAVESLNGHKFDD----KEWYVGRA 290

The following BLAST results are available for this feature:

BLAST of Spo05301.1 vs. NCBI nr
BLAST of Spo05301.1 vs. UniProtKB/TrEMBL
BLAST of Spo05301.1 vs. ExPASy Swiss-Prot
BLAST of Spo05301.1 vs. TAIR (Arabidopsis)

BLAST of Spo05301.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902094662\|gb\|KNA03373.1\|	5.2e-161	100.	hypothetical protein SOVF_2098...	[more]
gi\|902221241\|gb\|KNA18844.1\|	3.9e-156	96.5	hypothetical protein SOVF_0671...	[more]
gi\|731348810\|ref\|XP_010685677.1\|	1.4e-137	86.7	PREDICTED: eukaryotic translat...	[more]
gi\|224109462\|ref\|XP_002315204.1\|	6.0e-125	79.7	eukaryotic translation initiat...	[more]
gi\|702424313\|ref\|XP_010067450.1\|	5.1e-124	80.7	PREDICTED: eukaryotic translat...	[more]

BLAST of Spo05301.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q9K4_SPIOL	3.6e-161	100.	Eukaryotic translation initiat...	[more]
A0A0K9RIT4_SPIOL	2.7e-156	96.5	Eukaryotic translation initiat...	[more]
A0A0J8BVM2_BETVU	9.6e-138	86.7	Eukaryotic translation initiat...	[more]
B9HT66_POPTR	4.2e-125	79.7	Eukaryotic translation initiat...	[more]
A0A059BHS4_EUCGR	3.5e-124	80.7	Eukaryotic translation initiat...	[more]

BLAST of Spo05301.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
EI3GB_XENLA	8.0e-45	38.8	Eukaryotic translation initiat...	[more]
EIF3G_XENTR	2.3e-44	38.6	Eukaryotic translation initiat...	[more]
EI3GA_XENLA	3.1e-44	38.8	Eukaryotic translation initiat...	[more]
EIF3G_DANRE	2.0e-43	39.6	Eukaryotic translation initiat...	[more]
EIF3G_HUMAN	2.6e-43	41.3	Eukaryotic translation initiat...	[more]

BLAST of Spo05301.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G11400.2	2.8e-104	62.6	eukaryotic translation initiat...	[more]
AT5G06000.1	9.3e-92	62.1	eukaryotic translation initiat...	[more]
AT3G14100.1	3.6e-11	39.5	RNA-binding (RRM/RBD/RNP motif...	[more]
AT1G54080.2	5.2e-10	38.8	oligouridylate-binding protein...	[more]
AT4G34110.1	6.8e-10	40.1	poly(A) binding protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 205..271 score: 4.5
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 204..277 score: 4.3
IPR000504	RNA recognition motif domain	PROFILE	PS50102	RRM	coord: 203..281 score: 1
IPR012677	Nucleotide-binding alpha-beta plait domain	GENE3D	3.30.70.330		coord: 199..282 score: 5.5
IPR012677	Nucleotide-binding alpha-beta plait domain	unknown	SSF54928	RNA-binding domain, RBD	coord: 200..282 score: 2.69
IPR017334	Eukaryotic translation initiation factor 3 subunit G	HAMAP	MF_03006	eIF3g	coord: 5..283 score: 27
IPR017334	Eukaryotic translation initiation factor 3 subunit G	PIR	PIRSF037949	Transl_init_eIF-3_RNA-bind	coord: 1..284 score: 1.4
IPR017334	Eukaryotic translation initiation factor 3 subunit G	PANTHER	PTHR10352	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G	coord: 1..284 score: 7.7E
IPR024675	Eukaryotic translation initiation factor 3 subunit G, N-terminal	PFAM	PF12353	eIF3g	coord: 27..152 score: 7.4

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001731	formation of translation preinitiation complex
biological_process	GO:0006446	regulation of translational initiation
cellular_component	GO:0016282	eukaryotic 43S preinitiation complex
cellular_component	GO:0033290	eukaryotic 48S preinitiation complex
cellular_component	GO:0005852	eukaryotic translation initiation factor 3 complex
cellular_component	GO:0005840	ribosome
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0042393	histone binding
molecular_function	GO:0003676	nucleic acid binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo17936	0.70	Barchart \| Table
Spo06272	0.69	Barchart \| Table
Spo09090	0.68	Barchart \| Table
Spo12322	0.68	Barchart \| Table
Spo19751	0.67	Barchart \| Table
Spo06919	0.67	Barchart \| Table
Spo10042	0.67	Barchart \| Table
Spo03596	0.67	Barchart \| Table
Spo06506	0.65	Barchart \| Table