Homology
BLAST of Spo05258.1 vs. NCBI nr
Match:
gi|731366673|ref|XP_010695197.1| (PREDICTED: flavonoid 3'-monooxygenase [Beta vulgaris subsp. vulgaris])
HSP 1 Score: 892.5 bits (2305), Expect = 3.500e-256
Identity = 446/515 (86.60%), Postives = 471/515 (91.46%), Query Frame = 1
Query: 1 MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
MSPLE+ YTIA+AI I YYLITTI R+P PLPPGPKPWPIVGNLPHMGPVPHQGL
Sbjct: 1 MSPLELTFYTIALAIFI----YYLITTIFSRYPGPLPPGPKPWPIVGNLPHMGPVPHQGL 60
Query: 61 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
A LAQKYGPLL++RLGYVHVVVAASASVAA+FLK HDANFSSRPPNSGAK+IAYNYQD+V
Sbjct: 61 AKLAQKYGPLLHLRLGYVHVVVAASASVAAEFLKAHDANFSSRPPNSGAKYIAYNYQDLV 120
Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
FAPYG RWR+LRKISSLHLFS KA+EDYKPIRQEEV ILTKAL K+ S +QLGQLLNV
Sbjct: 121 FAPYGPRWRMLRKISSLHLFSAKALEDYKPIRQEEVAILTKALAKSGSNNPIQLGQLLNV 180
Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
CTTNALSR MLG+RVFGDGTG+SD KSEEFKDMVME+MVLAGEFNIGDFVPALEWLDLQG
Sbjct: 181 CTTNALSRAMLGRRVFGDGTGKSDTKSEEFKDMVMEVMVLAGEFNIGDFVPALEWLDLQG 240
Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
IA+KMKKLHKRFD FLGAILEEHK K G+ G DFLSTLISLKDN D E GKLTD EIK
Sbjct: 241 IASKMKKLHKRFDTFLGAILEEHKTNPK-GDHGRDFLSTLISLKDNYDDEDGKLTDTEIK 300
Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
ALLLNMFTAGTDTSSSTTEWAIAELIRHPKIL VQQELDSIVGQDRLVNESDLPNLPYF
Sbjct: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILDKVQQELDSIVGQDRLVNESDLPNLPYF 360
Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
QA++KEVFRLHPSTPLSLPR+SA+PCEINGYYIPKNTTLLVNVWAIARDPNVW+ PLEF+
Sbjct: 361 QAMIKEVFRLHPSTPLSLPRISADPCEINGYYIPKNTTLLVNVWAIARDPNVWSSPLEFQ 420
Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
PERFL GGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTA LVHGFDWKLA G
Sbjct: 421 PERFLPGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHGFDWKLADG 480
Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
L EKLNMEEAYGLTLQRK+PL V+PLPRL A AY
Sbjct: 481 LMSEKLNMEEAYGLTLQRKIPLAVHPLPRLSAHAY 510
BLAST of Spo05258.1 vs. NCBI nr
Match:
gi|1005743021|gb|AMQ23620.1| (flavonoid 3'hydroxylase [Silene littorea])
HSP 1 Score: 824.7 bits (2129), Expect = 9.000e-236
Identity = 410/518 (79.15%), Postives = 455/518 (87.84%), Query Frame = 1
Query: 1 MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
MS LE+ YTIAIA + YYL++T R+P+PLPPGPKPWPIVGNLPHMGP PHQGL
Sbjct: 1 MSTLELTFYTIAIATLL----YYLLSTTFHRYPKPLPPGPKPWPIVGNLPHMGPAPHQGL 60
Query: 61 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
AALAQKYGPLLYMRLGYVHVVVAASASVA+QFLK HD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61 AALAQKYGPLLYMRLGYVHVVVAASASVASQFLKVHDVNFSSRPPNSGAKHIAYNYQDLV 120
Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
FAPYG +WR+LRKI S+H+FS KA++D +RQEE IL AL A++K VQLGQLLNV
Sbjct: 121 FAPYGPKWRMLRKICSVHMFSNKALDDVTSVRQEEASILVNALANADAKP-VQLGQLLNV 180
Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
CTTNALSRVMLG+RV GDGTG+SD K+EEFK+MVMELMVL G FNIGDFVP LE LDLQG
Sbjct: 181 CTTNALSRVMLGRRVLGDGTGKSDPKAEEFKEMVMELMVLTGVFNIGDFVPVLECLDLQG 240
Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
IA+KMK LHKR DNF+GAILEEHKA K G GGD LSTLISLKDN DGEGG+ TD EIK
Sbjct: 241 IASKMKNLHKRLDNFMGAILEEHKANGKHG--GGDLLSTLISLKDNYDGEGGQFTDTEIK 300
Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
ALLL++F+AGTDTSSSTTEWA+AELIRHPKILA VQQELD++VG+DRLV ESD+PNL YF
Sbjct: 301 ALLLDLFSAGTDTSSSTTEWAMAELIRHPKILAQVQQELDTVVGRDRLVTESDIPNLTYF 360
Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
QAV+KEVFRLHPSTPLSLPR++AE CEINGY+IPKNTTLLVNVWAIARDP+VWA+PLEFR
Sbjct: 361 QAVIKEVFRLHPSTPLSLPRMAAESCEINGYHIPKNTTLLVNVWAIARDPDVWANPLEFR 420
Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQLLTA LVH ++WKLA G
Sbjct: 421 PERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLLTATLVHSYEWKLADG 480
Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTT 519
L EKLNMEEAYGLTLQRK PLMV+P+ RL A AY T
Sbjct: 481 LMVEKLNMEEAYGLTLQRKEPLMVHPVRRLSAHAYGPT 511
BLAST of Spo05258.1 vs. NCBI nr
Match:
gi|523496545|dbj|BAN62871.1| (flavonoid 3'-hydroxylase [Dianthus caryophyllus])
HSP 1 Score: 817.8 bits (2111), Expect = 1.100e-233
Identity = 405/519 (78.03%), Postives = 452/519 (87.09%), Query Frame = 1
Query: 1 MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
MSPLE+ YTI +A I YYLITT+ H +PLPPGP+PWPIVGNLPHMG PHQGL
Sbjct: 1 MSPLELTFYTIVLAT----ILYYLITTVFRGHQKPLPPGPRPWPIVGNLPHMGQAPHQGL 60
Query: 61 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
AALAQKYGPLLYMRLGYV VVVAASASVA QFLKTHD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61 AALAQKYGPLLYMRLGYVDVVVAASASVATQFLKTHDLNFSSRPPNSGAKHIAYNYQDLV 120
Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
FAPYG +WR+LRKI SLH+FS+KA++D++ +RQEEV IL A+ KA +K VQLGQLLNV
Sbjct: 121 FAPYGPKWRMLRKICSLHMFSSKALDDFRLVRQEEVSILVNAIAKAGTKP-VQLGQLLNV 180
Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
CTTNALSRVMLGKRV GDGTG+SD K+EEFKDMV+ELMVL G FNIGDFVPALE LDLQG
Sbjct: 181 CTTNALSRVMLGKRVLGDGTGKSDPKAEEFKDMVLELMVLTGVFNIGDFVPALECLDLQG 240
Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
+A+KMKKLHKR DNF+ ILEEHK+ N GGD LS LISLKDNCDGEGGK +D EIK
Sbjct: 241 VASKMKKLHKRLDNFMSNILEEHKSVAHQQN-GGDLLSILISLKDNCDGEGGKFSDTEIK 300
Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
ALLL++FTAGTDTSSSTTEWAIAELIRHPKILA VQQE+DS+VG+DRL+ E+D+PNL YF
Sbjct: 301 ALLLDLFTAGTDTSSSTTEWAIAELIRHPKILAQVQQEMDSVVGRDRLIAEADIPNLTYF 360
Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
QAV+KEVFRLHPSTPLSLPRV+ E CEINGY+IPKNTTLLVNVWAIARDP VWADPLEF+
Sbjct: 361 QAVIKEVFRLHPSTPLSLPRVANESCEINGYHIPKNTTLLVNVWAIARDPEVWADPLEFK 420
Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQL+TA L H +DW LA G
Sbjct: 421 PERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLMTATLAHTYDWALADG 480
Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY 520
L EKLNM+EAYGLTLQRKVPLMV+P RL A+ Y + +
Sbjct: 481 LMPEKLNMDEAYGLTLQRKVPLMVHPTRRLSARVYNSGF 513
BLAST of Spo05258.1 vs. NCBI nr
Match:
gi|470902337|dbj|BAN05297.1| (flavonoid 3'-hydroxylase [Dianthus caryophyllus])
HSP 1 Score: 813.5 bits (2100), Expect = 2.100e-232
Identity = 406/520 (78.08%), Postives = 452/520 (86.92%), Query Frame = 1
Query: 1 MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
MSPLE+ YTIA+A I YYLI T+ H +PLPPGP+PWPIVGNLPHMG PHQGL
Sbjct: 1 MSPLELTFYTIALAT----ILYYLIPTVFRGHQKPLPPGPRPWPIVGNLPHMGQAPHQGL 60
Query: 61 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
AALAQKYGPLLYMRLGYV VVVAASASVA QFLKTHD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61 AALAQKYGPLLYMRLGYVDVVVAASASVATQFLKTHDLNFSSRPPNSGAKHIAYNYQDLV 120
Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLL-N 180
FAPYG +WR+LRKI SLH+FS+KA++D++ +RQEEV IL A+ KA +K VQLGQLL N
Sbjct: 121 FAPYGPKWRMLRKICSLHMFSSKALDDFRLVRQEEVSILVNAIAKAGTKP-VQLGQLLLN 180
Query: 181 VCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQ 240
VCTTNALSRVMLGKRV GDGTG+SD K+EEFKDMV+ELMVL G FNIGDFVPALE LDLQ
Sbjct: 181 VCTTNALSRVMLGKRVLGDGTGKSDPKAEEFKDMVLELMVLTGVFNIGDFVPALECLDLQ 240
Query: 241 GIATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEI 300
G+A+KMKKLHKR DNF+ ILEEHK+ N GGD LSTLISLKDNCDGEGGK +D EI
Sbjct: 241 GVASKMKKLHKRLDNFMSNILEEHKSVAHQQN-GGDLLSTLISLKDNCDGEGGKFSDTEI 300
Query: 301 KALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPY 360
KALLL++FTAGTDTSSSTTEWAIAELIRHPKILA VQQE+DS+VG+DRL+ E+D+PNL Y
Sbjct: 301 KALLLDLFTAGTDTSSSTTEWAIAELIRHPKILAQVQQEMDSVVGRDRLIAEADIPNLTY 360
Query: 361 FQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEF 420
FQAV+KEVFRLHPSTPLSLPRV+ E CEINGY+IPKNTTLLVNVWAIARDP VWADPLEF
Sbjct: 361 FQAVIKEVFRLHPSTPLSLPRVANESCEINGYHIPKNTTLLVNVWAIARDPEVWADPLEF 420
Query: 421 RPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAH 480
+PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQL+TA L H +DW LA
Sbjct: 421 KPERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLMTATLAHTYDWALAD 480
Query: 481 GLAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY 520
GL EKLNM+EAYGLTLQRKVPLMV+P RL A Y + +
Sbjct: 481 GLMPEKLNMDEAYGLTLQRKVPLMVHPTRRLSAHVYNSGF 514
BLAST of Spo05258.1 vs. NCBI nr
Match:
gi|590668630|ref|XP_007037548.1| (Flavonoid 3'-hydroxylase isoform 2 [Theobroma cacao])
HSP 1 Score: 774.2 bits (1998), Expect = 1.400e-220
Identity = 380/503 (75.55%), Postives = 434/503 (86.28%), Query Frame = 1
Query: 14 AIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYM 73
+I A+ Y++ T+ RH PLPPGPKPWPI+GNLPHMGPVPH LAALA+ YGPL+++
Sbjct: 8 SILSAVFLYFIFATLRKRHRLPLPPGPKPWPIIGNLPHMGPVPHHSLAALAKVYGPLMHL 67
Query: 74 RLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWRLLRK 133
RLG+V VVVAASASVAAQFLK HDANFSSRPPNSGAK++AYNYQD+VFAPYG RWR+LRK
Sbjct: 68 RLGFVDVVVAASASVAAQFLKVHDANFSSRPPNSGAKYVAYNYQDLVFAPYGPRWRMLRK 127
Query: 134 ISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNALSRVMLGK 193
ISS+HLFS KA++D++ +RQ+EVG+L +AL A++K V LGQLLNVCT NAL RVMLGK
Sbjct: 128 ISSVHLFSGKALDDFRHVRQDEVGVLVRALADAKTK--VNLGQLLNVCTVNALGRVMLGK 187
Query: 194 RVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFD 253
RVFGDG+G++D +++EFK MV+ELMVLAG NIGDF+PALEWLDLQG+ KMKKLHKRFD
Sbjct: 188 RVFGDGSGKADPEADEFKSMVVELMVLAGVVNIGDFIPALEWLDLQGVQAKMKKLHKRFD 247
Query: 254 NFLGAILEEHKAKFK-GGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFTAGTD 313
FL AILEEHK K + G D LST ISL+D DGEGGKLTD EIKALLLNMFTAGTD
Sbjct: 248 RFLSAILEEHKIKARDGSGQHKDLLSTFISLED-ADGEGGKLTDTEIKALLLNMFTAGTD 307
Query: 314 TSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHP 373
TSSST EWAIAELIRHPKILA V++ELDS+VG+DRLV++ DLPNL YFQAV+KE FRLHP
Sbjct: 308 TSSSTVEWAIAELIRHPKILAQVRKELDSVVGRDRLVSDLDLPNLTYFQAVIKETFRLHP 367
Query: 374 STPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGGERPN 433
STPLSLPR+++E CEINGY+IPK TLLVNVWAIARDP+ W DPLEFRPERFL GGERPN
Sbjct: 368 STPLSLPRMASESCEINGYHIPKGATLLVNVWAIARDPDEWKDPLEFRPERFLPGGERPN 427
Query: 434 ADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAY 493
ADV+GNDFEVIPFGAGRRICAGMSLGLRMVQLL A LVH FDW+LA GL EKLNMEEA+
Sbjct: 428 ADVRGNDFEVIPFGAGRRICAGMSLGLRMVQLLAATLVHAFDWELADGLMPEKLNMEEAF 487
Query: 494 GLTLQRKVPLMVYPLPRLPAKAY 516
GLTLQR PLMV+P PRL +AY
Sbjct: 488 GLTLQRAAPLMVHPRPRLSPRAY 507
BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match:
A0A142D8F1_9CARY (Flavonoid 3'hydroxylase OS=Silene littorea PE=2 SV=1)
HSP 1 Score: 824.7 bits (2129), Expect = 6.300e-236
Identity = 410/518 (79.15%), Postives = 455/518 (87.84%), Query Frame = 1
Query: 1 MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
MS LE+ YTIAIA + YYL++T R+P+PLPPGPKPWPIVGNLPHMGP PHQGL
Sbjct: 1 MSTLELTFYTIAIATLL----YYLLSTTFHRYPKPLPPGPKPWPIVGNLPHMGPAPHQGL 60
Query: 61 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
AALAQKYGPLLYMRLGYVHVVVAASASVA+QFLK HD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61 AALAQKYGPLLYMRLGYVHVVVAASASVASQFLKVHDVNFSSRPPNSGAKHIAYNYQDLV 120
Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
FAPYG +WR+LRKI S+H+FS KA++D +RQEE IL AL A++K VQLGQLLNV
Sbjct: 121 FAPYGPKWRMLRKICSVHMFSNKALDDVTSVRQEEASILVNALANADAKP-VQLGQLLNV 180
Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
CTTNALSRVMLG+RV GDGTG+SD K+EEFK+MVMELMVL G FNIGDFVP LE LDLQG
Sbjct: 181 CTTNALSRVMLGRRVLGDGTGKSDPKAEEFKEMVMELMVLTGVFNIGDFVPVLECLDLQG 240
Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
IA+KMK LHKR DNF+GAILEEHKA K G GGD LSTLISLKDN DGEGG+ TD EIK
Sbjct: 241 IASKMKNLHKRLDNFMGAILEEHKANGKHG--GGDLLSTLISLKDNYDGEGGQFTDTEIK 300
Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
ALLL++F+AGTDTSSSTTEWA+AELIRHPKILA VQQELD++VG+DRLV ESD+PNL YF
Sbjct: 301 ALLLDLFSAGTDTSSSTTEWAMAELIRHPKILAQVQQELDTVVGRDRLVTESDIPNLTYF 360
Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
QAV+KEVFRLHPSTPLSLPR++AE CEINGY+IPKNTTLLVNVWAIARDP+VWA+PLEFR
Sbjct: 361 QAVIKEVFRLHPSTPLSLPRMAAESCEINGYHIPKNTTLLVNVWAIARDPDVWANPLEFR 420
Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQLLTA LVH ++WKLA G
Sbjct: 421 PERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLLTATLVHSYEWKLADG 480
Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTT 519
L EKLNMEEAYGLTLQRK PLMV+P+ RL A AY T
Sbjct: 481 LMVEKLNMEEAYGLTLQRKEPLMVHPVRRLSAHAYGPT 511
BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match:
S6B1I9_DIACA (Flavonoid 3'-hydroxylase OS=Dianthus caryophyllus GN=F3'H PE=3 SV=1)
HSP 1 Score: 817.8 bits (2111), Expect = 7.700e-234
Identity = 405/519 (78.03%), Postives = 452/519 (87.09%), Query Frame = 1
Query: 1 MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
MSPLE+ YTI +A I YYLITT+ H +PLPPGP+PWPIVGNLPHMG PHQGL
Sbjct: 1 MSPLELTFYTIVLAT----ILYYLITTVFRGHQKPLPPGPRPWPIVGNLPHMGQAPHQGL 60
Query: 61 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
AALAQKYGPLLYMRLGYV VVVAASASVA QFLKTHD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61 AALAQKYGPLLYMRLGYVDVVVAASASVATQFLKTHDLNFSSRPPNSGAKHIAYNYQDLV 120
Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
FAPYG +WR+LRKI SLH+FS+KA++D++ +RQEEV IL A+ KA +K VQLGQLLNV
Sbjct: 121 FAPYGPKWRMLRKICSLHMFSSKALDDFRLVRQEEVSILVNAIAKAGTKP-VQLGQLLNV 180
Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
CTTNALSRVMLGKRV GDGTG+SD K+EEFKDMV+ELMVL G FNIGDFVPALE LDLQG
Sbjct: 181 CTTNALSRVMLGKRVLGDGTGKSDPKAEEFKDMVLELMVLTGVFNIGDFVPALECLDLQG 240
Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
+A+KMKKLHKR DNF+ ILEEHK+ N GGD LS LISLKDNCDGEGGK +D EIK
Sbjct: 241 VASKMKKLHKRLDNFMSNILEEHKSVAHQQN-GGDLLSILISLKDNCDGEGGKFSDTEIK 300
Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
ALLL++FTAGTDTSSSTTEWAIAELIRHPKILA VQQE+DS+VG+DRL+ E+D+PNL YF
Sbjct: 301 ALLLDLFTAGTDTSSSTTEWAIAELIRHPKILAQVQQEMDSVVGRDRLIAEADIPNLTYF 360
Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
QAV+KEVFRLHPSTPLSLPRV+ E CEINGY+IPKNTTLLVNVWAIARDP VWADPLEF+
Sbjct: 361 QAVIKEVFRLHPSTPLSLPRVANESCEINGYHIPKNTTLLVNVWAIARDPEVWADPLEFK 420
Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQL+TA L H +DW LA G
Sbjct: 421 PERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLMTATLAHTYDWALADG 480
Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY 520
L EKLNM+EAYGLTLQRKVPLMV+P RL A+ Y + +
Sbjct: 481 LMPEKLNMDEAYGLTLQRKVPLMVHPTRRLSARVYNSGF 513
BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match:
M5AAC5_DIACA (Flavonoid 3'-hydroxylase OS=Dianthus caryophyllus PE=3 SV=1)
HSP 1 Score: 813.5 bits (2100), Expect = 1.400e-232
Identity = 406/520 (78.08%), Postives = 452/520 (86.92%), Query Frame = 1
Query: 1 MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
MSPLE+ YTIA+A I YYLI T+ H +PLPPGP+PWPIVGNLPHMG PHQGL
Sbjct: 1 MSPLELTFYTIALAT----ILYYLIPTVFRGHQKPLPPGPRPWPIVGNLPHMGQAPHQGL 60
Query: 61 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
AALAQKYGPLLYMRLGYV VVVAASASVA QFLKTHD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61 AALAQKYGPLLYMRLGYVDVVVAASASVATQFLKTHDLNFSSRPPNSGAKHIAYNYQDLV 120
Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLL-N 180
FAPYG +WR+LRKI SLH+FS+KA++D++ +RQEEV IL A+ KA +K VQLGQLL N
Sbjct: 121 FAPYGPKWRMLRKICSLHMFSSKALDDFRLVRQEEVSILVNAIAKAGTKP-VQLGQLLLN 180
Query: 181 VCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQ 240
VCTTNALSRVMLGKRV GDGTG+SD K+EEFKDMV+ELMVL G FNIGDFVPALE LDLQ
Sbjct: 181 VCTTNALSRVMLGKRVLGDGTGKSDPKAEEFKDMVLELMVLTGVFNIGDFVPALECLDLQ 240
Query: 241 GIATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEI 300
G+A+KMKKLHKR DNF+ ILEEHK+ N GGD LSTLISLKDNCDGEGGK +D EI
Sbjct: 241 GVASKMKKLHKRLDNFMSNILEEHKSVAHQQN-GGDLLSTLISLKDNCDGEGGKFSDTEI 300
Query: 301 KALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPY 360
KALLL++FTAGTDTSSSTTEWAIAELIRHPKILA VQQE+DS+VG+DRL+ E+D+PNL Y
Sbjct: 301 KALLLDLFTAGTDTSSSTTEWAIAELIRHPKILAQVQQEMDSVVGRDRLIAEADIPNLTY 360
Query: 361 FQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEF 420
FQAV+KEVFRLHPSTPLSLPRV+ E CEINGY+IPKNTTLLVNVWAIARDP VWADPLEF
Sbjct: 361 FQAVIKEVFRLHPSTPLSLPRVANESCEINGYHIPKNTTLLVNVWAIARDPEVWADPLEF 420
Query: 421 RPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAH 480
+PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQL+TA L H +DW LA
Sbjct: 421 KPERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLMTATLAHTYDWALAD 480
Query: 481 GLAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY 520
GL EKLNM+EAYGLTLQRKVPLMV+P RL A Y + +
Sbjct: 481 GLMPEKLNMDEAYGLTLQRKVPLMVHPTRRLSAHVYNSGF 514
BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match:
A0A061G4T4_THECC (Flavonoid 3'-hydroxylase isoform 2 OS=Theobroma cacao GN=TCM_014241 PE=3 SV=1)
HSP 1 Score: 774.2 bits (1998), Expect = 9.700e-221
Identity = 380/503 (75.55%), Postives = 434/503 (86.28%), Query Frame = 1
Query: 14 AIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYM 73
+I A+ Y++ T+ RH PLPPGPKPWPI+GNLPHMGPVPH LAALA+ YGPL+++
Sbjct: 8 SILSAVFLYFIFATLRKRHRLPLPPGPKPWPIIGNLPHMGPVPHHSLAALAKVYGPLMHL 67
Query: 74 RLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWRLLRK 133
RLG+V VVVAASASVAAQFLK HDANFSSRPPNSGAK++AYNYQD+VFAPYG RWR+LRK
Sbjct: 68 RLGFVDVVVAASASVAAQFLKVHDANFSSRPPNSGAKYVAYNYQDLVFAPYGPRWRMLRK 127
Query: 134 ISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNALSRVMLGK 193
ISS+HLFS KA++D++ +RQ+EVG+L +AL A++K V LGQLLNVCT NAL RVMLGK
Sbjct: 128 ISSVHLFSGKALDDFRHVRQDEVGVLVRALADAKTK--VNLGQLLNVCTVNALGRVMLGK 187
Query: 194 RVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFD 253
RVFGDG+G++D +++EFK MV+ELMVLAG NIGDF+PALEWLDLQG+ KMKKLHKRFD
Sbjct: 188 RVFGDGSGKADPEADEFKSMVVELMVLAGVVNIGDFIPALEWLDLQGVQAKMKKLHKRFD 247
Query: 254 NFLGAILEEHKAKFK-GGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFTAGTD 313
FL AILEEHK K + G D LST ISL+D DGEGGKLTD EIKALLLNMFTAGTD
Sbjct: 248 RFLSAILEEHKIKARDGSGQHKDLLSTFISLED-ADGEGGKLTDTEIKALLLNMFTAGTD 307
Query: 314 TSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHP 373
TSSST EWAIAELIRHPKILA V++ELDS+VG+DRLV++ DLPNL YFQAV+KE FRLHP
Sbjct: 308 TSSSTVEWAIAELIRHPKILAQVRKELDSVVGRDRLVSDLDLPNLTYFQAVIKETFRLHP 367
Query: 374 STPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGGERPN 433
STPLSLPR+++E CEINGY+IPK TLLVNVWAIARDP+ W DPLEFRPERFL GGERPN
Sbjct: 368 STPLSLPRMASESCEINGYHIPKGATLLVNVWAIARDPDEWKDPLEFRPERFLPGGERPN 427
Query: 434 ADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAY 493
ADV+GNDFEVIPFGAGRRICAGMSLGLRMVQLL A LVH FDW+LA GL EKLNMEEA+
Sbjct: 428 ADVRGNDFEVIPFGAGRRICAGMSLGLRMVQLLAATLVHAFDWELADGLMPEKLNMEEAF 487
Query: 494 GLTLQRKVPLMVYPLPRLPAKAY 516
GLTLQR PLMV+P PRL +AY
Sbjct: 488 GLTLQRAAPLMVHPRPRLSPRAY 507
BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match:
A0A0H3WMD5_CAMSI (Flavonoid 3'-hydroxylase 2 OS=Camellia sinensis GN=F3'-H2 PE=2 SV=1)
HSP 1 Score: 768.5 bits (1983), Expect = 5.300e-219
Identity = 377/517 (72.92%), Postives = 434/517 (83.95%), Query Frame = 1
Query: 1 MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
M+ L + Y++ IA+ + + L T + RHPRPLPPGP PWP++GNLPH+G +PH +
Sbjct: 1 MTSLAFVLYSLLIAVFLYCLLN-LRTLLFNRHPRPLPPGPTPWPVIGNLPHLGTMPHHSI 60
Query: 61 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
A +A+ YGPL+++RLG V VVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61 ATMARTYGPLMHLRLGLVDVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDLV 120
Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
FAPYG RWR+LRKI S+HLFSTKA++D++ +RQEE+ ILT+AL A AV LGQLLNV
Sbjct: 121 FAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQEEIAILTRALESAGQAAAVNLGQLLNV 180
Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
CTTNAL RVMLG RVFGDG+G D K++EFK+MV+ELMVLAG FNIGDFVPALEWLDLQG
Sbjct: 181 CTTNALGRVMLGHRVFGDGSGSGDPKADEFKEMVVELMVLAGVFNIGDFVPALEWLDLQG 240
Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFK--GGNFGGDFLSTLISLKDNCDGEGGKLTDIE 300
+A+KMKKLH RFD+FL ILEEHK + G D LSTLISLKD+ DGEGGKLTDIE
Sbjct: 241 VASKMKKLHARFDSFLNKILEEHKVNYSSSGAQKHTDLLSTLISLKDDADGEGGKLTDIE 300
Query: 301 IKALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLP 360
IKALLL++FTAGTDTSSST EWAIAELIRHPKILA +QELDSIVG DR V ESDL L
Sbjct: 301 IKALLLDLFTAGTDTSSSTVEWAIAELIRHPKILAQAKQELDSIVGPDRRVTESDLAQLT 360
Query: 361 YFQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLE 420
+ QA++KE FRLHPSTPLSLPR++++ CEINGY+IPK +TLLVNVWAIARDP+ WA+PLE
Sbjct: 361 FLQAIIKETFRLHPSTPLSLPRMASDSCEINGYFIPKGSTLLVNVWAIARDPDAWAEPLE 420
Query: 421 FRPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLA 480
FRPERFL GGE+PNADV+GNDFEVIPFGAGRRICAGMSLGLRMVQLLTA LVH FDW LA
Sbjct: 421 FRPERFLPGGEKPNADVRGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHAFDWDLA 480
Query: 481 HGLAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
G + EKLNM+EAYGLTLQR PLMV+P PRL Y
Sbjct: 481 DGQSTEKLNMDEAYGLTLQRAAPLMVHPRPRLAPHVY 516
BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match:
F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)
HSP 1 Score: 724.9 bits (1870), Expect = 6.100e-208
Identity = 356/513 (69.40%), Postives = 422/513 (82.26%), Query Frame = 1
Query: 4 LEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAAL 63
+EI+S + I ++ + L + R+P PLPPGPKPWPI+GNL H+GP PHQ AA+
Sbjct: 1 MEILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAM 60
Query: 64 AQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAP 123
AQ YGPL+Y+++G+V VVVAASASVAAQFLKTHDANFSSRPPNSGA+H+AYNYQD+VFAP
Sbjct: 61 AQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAP 120
Query: 124 YGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTT 183
YG RWR+LRKI S+HLFSTKA++D++ +RQ+EV LT+AL A K V+LGQLLNVCTT
Sbjct: 121 YGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKP-VKLGQLLNVCTT 180
Query: 184 NALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIAT 243
NAL+RVMLGKRVF DG+G+ D ++ EFK MV+E+MV+AG FNIGDF+P L WLD+QG+A
Sbjct: 181 NALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAA 240
Query: 244 KMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLK-DNCDGEGGKLTDIEIKAL 303
KMKKLH RFD FL ILEEHK K G D LSTLISLK D+ D +GGKLTD EIKAL
Sbjct: 241 KMKKLHARFDAFLTDILEEHKGKIFGEM--KDLLSTLISLKNDDADNDGGKLTDTEIKAL 300
Query: 304 LLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQA 363
LLN+F AGTDTSSST EWAIAELIR+PKILA QQE+D +VG+DRLV E DL L Y +A
Sbjct: 301 LLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEA 360
Query: 364 VMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPE 423
++KE FRLHPSTPLSLPR+++E CEINGY+IPK +TLL+NVWAIARDPN WADPLEFRPE
Sbjct: 361 IVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPE 420
Query: 424 RFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLA 483
RFL GGE+P DV+GNDFEVIPFGAGRRICAGM+LG+RMVQL+ A L+H F+W L G
Sbjct: 421 RFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQL 480
Query: 484 QEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
E LNMEEAYGLTLQR PL+V+P PRL A+AY
Sbjct: 481 PEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510
BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match:
F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)
HSP 1 Score: 677.9 bits (1748), Expect = 8.500e-194
Identity = 333/511 (65.17%), Postives = 406/511 (79.45%), Query Frame = 1
Query: 10 TIAIAIAIAIITYYLITTIIFR----HPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQ 69
T+ + I +A + + ++ R H LPPGP PWPI+GNLPHMG PH+ L+A+
Sbjct: 3 TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVT 62
Query: 70 KYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYG 129
YGP+L++RLG+V VVVAAS SVA QFLK HDANF+SRPPNSGAKH+AYNYQD+VFAPYG
Sbjct: 63 TYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYG 122
Query: 130 QRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNA 189
RWRLLRKISS+HLFS KA+ED+K +RQEEVG LT+ LV+ +K V LGQL+N+C NA
Sbjct: 123 HRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKP-VNLGQLVNMCVVNA 182
Query: 190 LSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKM 249
L R M+G+R+FG ++D K++EF+ MV E+M LAG FNIGDFVP+L+WLDLQG+A KM
Sbjct: 183 LGREMIGRRLFG---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKM 242
Query: 250 KKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKD-NCDGEGGKLTDIEIKALLL 309
K+LHKRFD FL +IL+EH+ + D LSTLISLK + DG+GG LTD EIKALLL
Sbjct: 243 KRLHKRFDAFLSSILKEHEMNGQDQKHT-DMLSTLISLKGTDLDGDGGSLTDTEIKALLL 302
Query: 310 NMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVM 369
NMFTAGTDTS+ST +WAIAELIRHP I+ Q+ELD +VG+DR VNESD+ LPY QAV+
Sbjct: 303 NMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVI 362
Query: 370 KEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERF 429
KE FRLHP TPLSLP +++E CEINGY+IPK +TLL N+WAIARDP+ W+DPL F+PERF
Sbjct: 363 KENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERF 422
Query: 430 LKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQE 489
L GGE+ DVKG+DFE+IPFGAGRRICAG+SLGLR +Q LTA LV GFDW+LA G+ E
Sbjct: 423 LPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPE 482
Query: 490 KLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
KLNMEE+YGLTLQR VPL+V+P PRL Y
Sbjct: 483 KLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508
BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match:
C75A1_PETHY (Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1)
HSP 1 Score: 521.2 bits (1341), Expect = 1.300e-146
Identity = 261/507 (51.48%), Postives = 352/507 (69.43%), Query Frame = 1
Query: 11 IAIAIAIAIITYYLITTIIFRHP-RPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGP 70
+ A +I +I + +I+T+I + R LPPGP+ WP++G LP +G +PH LA +A+KYG
Sbjct: 7 LGAATSIFLIAHIIISTLISKTTGRHLPPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGA 66
Query: 71 LLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWR 130
++Y+++G + VA++ A FLKT D NFS+RPPN+GA H+AYN QDMVFA YG RW+
Sbjct: 67 IMYLKVGTCGMAVASTPDAAKAFLKTLDINFSNRPPNAGATHLAYNAQDMVFAHYGPRWK 126
Query: 131 LLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESK-EAVQLGQLLNVCTTNALSR 190
LLRK+S+LH+ KA+E++ +R E+G + K++ + + V + ++L N + +
Sbjct: 127 LLRKLSNLHMLGGKALENWANVRANELGHMLKSMSDMSREGQRVVVAEMLTFAMANMIGQ 186
Query: 191 VMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKL 250
VML KRVF D E + EFKDMV+ELM +AG FNIGDF+P L W+DLQGI +MK+L
Sbjct: 187 VMLSKRVFVDKGVEVN----EFKDMVVELMTIAGYFNIGDFIPCLAWMDLQGIEKRMKRL 246
Query: 251 HKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFT 310
HK+FD L + +EHKA DFL ++ DN +GE +L+ IKALLLN+FT
Sbjct: 247 HKKFDALLTKMFDEHKATTYERKGKPDFLDVVMENGDNSEGE--RLSTTNIKALLLNLFT 306
Query: 311 AGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVF 370
AGTDTSSS EWA+AE++++P IL Q E+D ++G++R + ESD+PNLPY +A+ KE F
Sbjct: 307 AGTDTSSSAIEWALAEMMKNPAILKKAQAEMDQVIGRNRRLLESDIPNLPYLRAICKETF 366
Query: 371 RLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGG 430
R HPSTPL+LPR+S EPC ++GYYIPKNT L VN+WAI RDP VW +PLEF PERFL G
Sbjct: 367 RKHPSTPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPQVWENPLEFNPERFLSG- 426
Query: 431 ERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNM 490
D +GNDFE+IPFGAGRRICAG +G+ MV+ + LVH FDWKL + + LNM
Sbjct: 427 RNSKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDWKLPSEVIE--LNM 486
Query: 491 EEAYGLTLQRKVPLMVYPLPRLPAKAY 516
EEA+GL LQ+ VPL PRL Y
Sbjct: 487 EEAFGLALQKAVPLEAMVTPRLQLDVY 504
BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match:
C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)
HSP 1 Score: 520.4 bits (1339), Expect = 2.300e-146
Identity = 266/517 (51.45%), Postives = 359/517 (69.44%), Query Frame = 1
Query: 3 PLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAA 62
P E+I TI I I + II LI T +R R LPPGP+ WP++G LP +G +PH LA
Sbjct: 5 PSELIGATI-IYIIVYIIIQKLIATGSWRRRR-LPPGPEGWPVIGALPLLGGMPHVALAK 64
Query: 63 LAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFA 122
+A+KYGP++Y+++G +VVA++ + A FLKT D NFS+RPPN+GA H+AYN QDMVFA
Sbjct: 65 MAKKYGPIMYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRPPNAGATHMAYNAQDMVFA 124
Query: 123 PYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESK-EAVQLGQLLNVC 182
PYG RW+LLRK+S+LH+ KA+E++ +R E+G + K++ A E + + +L
Sbjct: 125 PYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASHVGERIVVADMLTFA 184
Query: 183 TTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGI 242
N + +VML KRVF E K+ EFK+MV+ELM +AG FNIGDF+P + W+DLQGI
Sbjct: 185 MANMIGQVMLSKRVFV----EKGKEVNEFKNMVVELMTVAGYFNIGDFIPQIAWMDLQGI 244
Query: 243 ATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKA 302
MKKLHK+FD+ L + EEH+A DFL +++ +DN +GE +L+ IKA
Sbjct: 245 EKGMKKLHKKFDDLLTKMFEEHEATSNERKGKPDFLDFIMANRDNSEGE--RLSITNIKA 304
Query: 303 LLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQ 362
LLLN+FTAGTDTSSS EWA+ E++++P I QQE+D I+G++R ESD+PNLPY +
Sbjct: 305 LLLNLFTAGTDTSSSVIEWALTEMMKNPTIFKKAQQEMDQIIGKNRRFIESDIPNLPYLR 364
Query: 363 AVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRP 422
A+ KE FR HPSTPL+LPRVS++ C I+GYYIPKNT L VN+WAI RDP+VW +PLEF P
Sbjct: 365 AICKEAFRKHPSTPLNLPRVSSDACTIDGYYIPKNTRLSVNIWAIGRDPDVWENPLEFIP 424
Query: 423 ERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGL 482
ERFL + + +GNDFE+IPFGAGRRICAG +G+ MV+ + L+H FDWKL + +
Sbjct: 425 ERFL-SEKNAKIEHRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLIHSFDWKLPNDV 484
Query: 483 AQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTT 519
+NMEE +GL LQ+ VPL PRL Y ++
Sbjct: 485 VD--INMEETFGLALQKAVPLEAIVTPRLSFDIYQSS 510
BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match:
C75A3_PETHY (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1)
HSP 1 Score: 518.1 bits (1333), Expect = 1.100e-145
Identity = 261/507 (51.48%), Postives = 350/507 (69.03%), Query Frame = 1
Query: 11 IAIAIAIAIITYYLITTII-FRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGP 70
+A A I + T+ I+T++ + R LPPGP+ WP++G LP +G +PH LA +A+KYG
Sbjct: 7 LAAATLIFLTTHIFISTLLSITNGRRLPPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGA 66
Query: 71 LLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWR 130
++Y+++G +VVA++ A FLKT D NFS+RPPN+GA H+AY QDMVFA YG RW+
Sbjct: 67 IMYLKVGTCGMVVASTPDAAKAFLKTLDLNFSNRPPNAGATHLAYGAQDMVFAHYGPRWK 126
Query: 131 LLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESK-EAVQLGQLLNVCTTNALSR 190
LLRK+S+LH+ KA+E++ +R E+G + K++ + E V + ++L N + +
Sbjct: 127 LLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDMSREGERVVVAEMLTFAMANMIGQ 186
Query: 191 VMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKL 250
V+L KRVF + E + EFKDMV+ELM AG FNIGDF+P L W+DLQGI MK+L
Sbjct: 187 VILSKRVFVNKGVEVN----EFKDMVVELMTTAGYFNIGDFIPCLAWMDLQGIEKGMKRL 246
Query: 251 HKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFT 310
HK+FD L + +EHKA DFL ++ +DN +GE +L+ IKALLLN+FT
Sbjct: 247 HKKFDALLTKMFDEHKATSYERKGKPDFLDCVMENRDNSEGE--RLSTTNIKALLLNLFT 306
Query: 311 AGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVF 370
AGTDTSSS EWA+AE++++P IL Q E+D ++G +R + ESD+PNLPY +A+ KE F
Sbjct: 307 AGTDTSSSAIEWALAEMMKNPAILKKAQGEMDQVIGNNRRLLESDIPNLPYLRAICKETF 366
Query: 371 RLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGG 430
R HPSTPL+LPR+S EPC ++GYYIPKNT L VN+WAI RDP VW +PLEF PERFL G
Sbjct: 367 RKHPSTPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPEVWENPLEFYPERFLSG- 426
Query: 431 ERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNM 490
D +GNDFE+IPFGAGRRICAG +G+ MV+ + LVH FDWKL + + LNM
Sbjct: 427 RNSKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDWKLPSEVIE--LNM 486
Query: 491 EEAYGLTLQRKVPLMVYPLPRLPAKAY 516
EEA+GL LQ+ VPL PRLP Y
Sbjct: 487 EEAFGLALQKAVPLEAMVTPRLPIDVY 504
BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match:
AT5G07990.1 (Cytochrome P450 superfamily protein)
HSP 1 Score: 677.9 bits (1748), Expect = 4.800e-195
Identity = 333/511 (65.17%), Postives = 406/511 (79.45%), Query Frame = 1
Query: 10 TIAIAIAIAIITYYLITTIIFR----HPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQ 69
T+ + I +A + + ++ R H LPPGP PWPI+GNLPHMG PH+ L+A+
Sbjct: 3 TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVT 62
Query: 70 KYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYG 129
YGP+L++RLG+V VVVAAS SVA QFLK HDANF+SRPPNSGAKH+AYNYQD+VFAPYG
Sbjct: 63 TYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYG 122
Query: 130 QRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNA 189
RWRLLRKISS+HLFS KA+ED+K +RQEEVG LT+ LV+ +K V LGQL+N+C NA
Sbjct: 123 HRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKP-VNLGQLVNMCVVNA 182
Query: 190 LSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKM 249
L R M+G+R+FG ++D K++EF+ MV E+M LAG FNIGDFVP+L+WLDLQG+A KM
Sbjct: 183 LGREMIGRRLFG---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKM 242
Query: 250 KKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKD-NCDGEGGKLTDIEIKALLL 309
K+LHKRFD FL +IL+EH+ + D LSTLISLK + DG+GG LTD EIKALLL
Sbjct: 243 KRLHKRFDAFLSSILKEHEMNGQDQKHT-DMLSTLISLKGTDLDGDGGSLTDTEIKALLL 302
Query: 310 NMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVM 369
NMFTAGTDTS+ST +WAIAELIRHP I+ Q+ELD +VG+DR VNESD+ LPY QAV+
Sbjct: 303 NMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVI 362
Query: 370 KEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERF 429
KE FRLHP TPLSLP +++E CEINGY+IPK +TLL N+WAIARDP+ W+DPL F+PERF
Sbjct: 363 KENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERF 422
Query: 430 LKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQE 489
L GGE+ DVKG+DFE+IPFGAGRRICAG+SLGLR +Q LTA LV GFDW+LA G+ E
Sbjct: 423 LPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPE 482
Query: 490 KLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
KLNMEE+YGLTLQR VPL+V+P PRL Y
Sbjct: 483 KLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508
BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match:
AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6)
HSP 1 Score: 364.0 bits (933), Expect = 1.500e-100
Identity = 201/512 (39.26%), Postives = 299/512 (58.40%), Query Frame = 1
Query: 4 LEIISYTIAIAIAIAIITYYLITTIIFRHPRP-LPPGPKPWPIVGNLPHMGPVPHQGLAA 63
+ + Y I I IAI I +YL R P+P LPPGP+ PIVGNLP + P H
Sbjct: 13 INLTPYAILILIAIFSILWYLFK----RSPQPHLPPGPRGLPIVGNLPFLDPDLHTYFTK 72
Query: 64 LAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFA 123
LA+ YGP+ + LG VV + S+A + LK D NFS+ A+ + Y D+V+
Sbjct: 73 LAESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWL 132
Query: 124 PYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALV-KAESKEAVQLGQLLNVC 183
PYG WR+LRK+ L L S + + + +R++E+ T+ L K + + V +G+ + +
Sbjct: 133 PYGAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLT 192
Query: 184 TTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGI 243
N ++ G V + E + EFK+++ E+ L GE N+ DF P L DLQG+
Sbjct: 193 MMNLTMNMLWGGSVKAE---EMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGL 252
Query: 244 ATKMKKLHKRFDNFLGAILEEHKA-KFKGGNFGG--DFLSTLISLKDNCDGEGGKLTDIE 303
KM + D L +E+ + + + G+ G DFL L+ LKD +T
Sbjct: 253 VKKMHVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNH 312
Query: 304 IKALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLP 363
+KA+L+++ GTDTS++T E+A+AELIR P+++ QQELD +VG+D ++ ES + LP
Sbjct: 313 VKAVLVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLP 372
Query: 364 YFQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLE 423
+ A+MKE RL+P+ PL +P +E + GY IPKNT + +NVW+I RDPNVW P E
Sbjct: 373 FISAIMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTE 432
Query: 424 FRPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLA 483
FRPERFL ++ + D G D+ +PFG+GRRICAG++L RM+ A L+H FDWK+
Sbjct: 433 FRPERFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIP 492
Query: 484 HGLAQEKLNMEEAYGLTLQRKVPLMVYPLPRL 511
G L+++E +G+ L+ K PL+ P+PRL
Sbjct: 493 EG---HILDLKEKFGIVLKLKSPLVALPVPRL 511
BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match:
AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2)
HSP 1 Score: 361.3 bits (926), Expect = 9.900e-100
Identity = 192/489 (39.26%), Postives = 288/489 (58.90%), Query Frame = 1
Query: 36 LPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKT 95
LPPGP PI+GNL +GP+PH+ LA+L KYGPL+Y+RLG V + + L
Sbjct: 32 LPPGPPRLPILGNLLQLGPLPHRDLASLCDKYGPLVYLRLGNVDAITTNDPDTIREILLR 91
Query: 96 HDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEE 155
D FSSRP A H+AY D+ AP G W+ +R+I HL +TK +E + R EE
Sbjct: 92 QDDVFSSRPKTLAAVHLAYGCGDVALAPMGPHWKRMRRICMEHLLTTKRLESFTTQRAEE 151
Query: 156 VGILTKALVK-AESKEAVQLGQLLNVCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMV 215
L + + K +E+ + + L ++L + N ++R++LGK+ FG G+ S K+++EF +
Sbjct: 152 ARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLGKQFFGPGSLVSPKEAQEFLHIT 211
Query: 216 MELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFDNFLGAILEEHK-AKFKGGNFG 275
+L L G +GD++P W+D G +M+ + KR D F I++EH+ AK + +
Sbjct: 212 HKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKN 271
Query: 276 GD--FLSTLISLKDNCDGEGGK--LTDIEIKALLLNMFTAGTDTSSSTTEWAIAELIRHP 335
GD F+ L+SL GE GK + D+EIKAL+ +M A TDTS+ T EWA+AE I+ P
Sbjct: 272 GDMDFVDVLLSLP----GENGKAHMEDVEIKALIQDMIAAATDTSAVTNEWAMAEAIKQP 331
Query: 336 KILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHPSTPLSLPRVSAEPCEIN 395
+++ +Q+ELD++VG +R+V+ESDL +L Y + V++E FR+HP+ P +P S IN
Sbjct: 332 RVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMHPAGPFLIPHESVRATTIN 391
Query: 396 GYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERF--LKGGERPNADVKGNDFEVIPFGA 455
GYYIP T + +N + R+ +W D +FRPER ++G R G DF+++PF A
Sbjct: 392 GYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSGRVEIS-HGPDFKILPFSA 451
Query: 456 GRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAYGLTLQRKVPLMVYPL 515
G+R C G LG+ MV + A L H F+W + ++ E YG+T+ + PL
Sbjct: 452 GKRKCPGAPLGVTMVLMALARLFHCFEWS-----SPGNIDTVEVYGMTMPKAKPLRAIAK 510
Query: 516 PRLPAKAYT 517
PRL A YT
Sbjct: 512 PRLAAHLYT 510
BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match:
AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5)
HSP 1 Score: 360.5 bits (924), Expect = 1.700e-99
Identity = 201/514 (39.11%), Postives = 300/514 (58.37%), Query Frame = 1
Query: 3 PLEIISYT-IAIAIAIAIITYYLITTIIFRHPRP-LPPGPKPWPIVGNLPHMGPVPHQGL 62
P IS T A A+ I T+ ++ I R P+P LPPGP+ PIVGNLP + P H
Sbjct: 9 PDNAISLTPYAYAVLILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLDPDLHTYF 68
Query: 63 AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 122
LAQ +GP+ + LG VV S S+A++ LK D NFS+ A+ + Y D+V
Sbjct: 69 TKLAQSHGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLV 128
Query: 123 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALV-KAESKEAVQLGQLLN 182
+ PYG WR+LRK+ + LFS K ++ + +R++E+ T+ L K K V +G+ L
Sbjct: 129 WLPYGAEWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLF 188
Query: 183 VCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQ 242
+ N + ++ G V + + + EFK ++ E+ L G N+ DF P L DLQ
Sbjct: 189 LTMMNLMMNMLWGGSVKAE---DMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQ 248
Query: 243 GIATKMKKLHKRFDNFLGAILEE-HKAKFKGGNFGG--DFLSTLISLKDNCDGEGGKLTD 302
G+ KM + D L +E+ + + + G+ G DFL L+ L+D +T
Sbjct: 249 GLVKKMHLYARDLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITM 308
Query: 303 IEIKALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPN 362
+KA+L++M GT++S++T E+ +AELI +P+++ QQELD +VG+D +V ES + +
Sbjct: 309 NHVKAVLMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITS 368
Query: 363 LPYFQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADP 422
LPY AV+KE RL+P+ PL +P +E + GY IPKNT + +NVW+I RDPNVW P
Sbjct: 369 LPYILAVLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYP 428
Query: 423 LEFRPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWK 482
EFRPERFL ++ + D G D+ +PFG+GRRICAG++L RM+ A L+H FDW
Sbjct: 429 TEFRPERFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWT 488
Query: 483 LAHGLAQEKLNMEEAYGLTLQRKVPLMVYPLPRL 511
+ G L++EE +G+ L+ K PL+ P+PRL
Sbjct: 489 IPDG---HVLDLEEKFGIVLKLKTPLVALPIPRL 513
BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match:
AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4)
HSP 1 Score: 358.6 bits (919), Expect = 6.400e-99
Identity = 194/482 (40.25%), Postives = 290/482 (60.17%), Query Frame = 1
Query: 36 LPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKT 95
LPPGP PI+GN+ +G PH A LA+ YGP++ ++ G ++ VV S A + L+T
Sbjct: 38 LPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRT 97
Query: 96 HDANFSSRPPNSGAKHIAYNYQDMVFAPYGQ-RWRLLRKISSLHLFSTKAMEDYKPIRQE 155
HD S R N + + +++ P RWR+LRK+S +FS + E K +R +
Sbjct: 98 HDQILSGRKSNDSIRCFGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMK 157
Query: 156 EVGILTKALVKA-ESKEAVQLGQLLNVCTTNALSRVMLGKRVFGDGTGESD-KKSEEFKD 215
+V L + ++ E KEAV + + N +S ++ F G D KKS EF+D
Sbjct: 158 KVQELVSFMNESSERKEAVDISRASYTTVLNIISNIL-----FSVDLGSYDSKKSNEFQD 217
Query: 216 MVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFDNFLGAILEEHKAKFKGGNF 275
V+ M AG+ + ++ P + +LDLQG M+ L +R ++ A+ GN+
Sbjct: 218 TVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDAKIAEKSLGNY 277
Query: 276 GGD-----FLSTLISLKDNCDGEGGKLTDIEIKALLLNMFTAGTDTSSSTTEWAIAELIR 335
D FL +L+ L + G+ +L + +I+ LLL+MFTAGTDTSSST EWA+AEL+R
Sbjct: 278 SKDVSNRDFLDSLLILNE---GDEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAMAELLR 337
Query: 336 HPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHPSTPLSLPRVSAEPCE 395
+PK + Q E+D ++GQ+ +V ESD+ LPY QAV+KE FRLHP+ PL +PR + E
Sbjct: 338 NPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPLLVPRKAESDVE 397
Query: 396 INGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGGERPNADVKGNDFEVIPFGA 455
+ G+ +PK+T +LVNVWAI RDP+VW +P +F PERF+ + DVKG D+E+ PFG
Sbjct: 398 VLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFM----GKDIDVKGRDYELTPFGG 457
Query: 456 GRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAYGLTLQRKVPLMVYPL 510
GRRIC G+ L ++ V L+ A+L++ FDWKL +G+ E L+M+E +G+TL R L P+
Sbjct: 458 GRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLHRTNTLYAIPV 507
The following BLAST results are available for this feature:
