Spo04534 (gene)

Overview
NameSpo04534
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionGlucose-methanol-choline (GMC) oxidoreductase family protein
Locationchr2 : 3121042 .. 3124596 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGTCTAAATTTGGATCTTGTCGGACCCGATCCAGATCCGACTCATTGTTATCCGTATCCTTACTTGACTACTTCCATTCCACGCTGAAAAGAAAATGCAGCCCAATAAGCGTTGCGGTGACCATAAATGCAGCCCGATAGTGTCATCTTTACGAGGCAAACTCGAATAAATGTTCAAAAACTATAAACGCGGGTTAATTGCACACAAGACAATACATACACAAACGGGATTGCCAAAATGCAAAGGCTGTTAAGAATACAACATTCTTTTGGTTCATTATTAAAAAATCTTCCTGCAAAACCAGTTTCTAACCGAATTTCTAGCGACACCCCCCTACCCCCTTTCCCCACTTTCTCTCTCCTCCATTTCTCTCTGTCTACAGTGGCTTCTGCAACTCTGTAGCTGACTACTAGCTGGGCATTGGGGATTACCCATTATCTTTTTCTTGATTACCCATTTCTATCTTCTTCAATTAAACACCAAGAAAAGGAGATATTTCAAAAGAAAATGTCGAAATTGGTGATACCCAGATTGTTTAATGACGAATTCTTCAGTAGATTGCTCGTAATTTTGATCTTGCTCACATTCTTTGGGTGTTGCACAGCTCAACCACAAGAGGGTATTTCTTATTTCTTATAATTCAAGCTTTTATTTCCTCATTTTTGTTTATGCCCACCACCTTTTCGATGAAATGCTACTGGAAGAACTCTTCTGTTTCCAAGATTTATATGATACCCTTTTTCTTGGGTAGTTTAATGCATGTTTTAAGGGTTTTAATTATGAACTTTTTGATTTCCCCACTATATTACCTTGCTATATAAATAGTTCGAGTGTTGTAGTTGATGTATTACGTTGAAATATGTTGTGCAATTCTTGTCTTAGAACTTGTTACGTTTCCAGTTGATGTATTACGTTGAAATAGATTTAGATGTCAAAAATAATTTATTGCTTAATTATACCGTCTCTATATTGTGAATTTAGCTTAGCTGTTTCGTCAATGATGTTTTGAGTTCATATTTTAATACGATTTCGAAAATTCGAAGTCAATTGCTTTTCACATACTTGGGTGATCCATGGAATGGAAGAGAAAGTAGCCTGCAGAGTGTATGTAGCTTTTGATACTTTTCACCTATTTTCCTGTAGACAATCTGTTATAAATAACCTAGCGAAAATATGTTCAACAATTTACAGTATTTATGCTAAAACTATGAAGACACTTTCTTATCCACCTGAATCAACTAGCAAACTTTTTATGAATATAAGTATGATATGTGTAACTTGTATATTTCTTGAAAAAAAAAAATGCGTCATAGAAGATTTGAGAGCCTCTTCAATCTAGACATACCGTCCACTGTCTTCACTAAATCTGCATGACAGTTACAGAACAAATTATCACTTTGACACAGTGCTTCGTTATGGATTATGTAATTGGATCTAGGACTAAATGAGAGTATTCATTTTTATTGTTCATTTAGAAGCAGCAGTAGATATGCATTAGTCAGTTGACTGCTGGAGTAATGCTGGCAACTTTTCTGAGTGTTCTTACCATGGGAAAGTGTTGTGTAGTCTTCCACTATAGTGCATAACTTACTGATGCAAATTAAATGCCCTCTGCAGCTCCTGCCTATCTTCAATTTACTGCAAATGCTACTGAATTCCCTGCGGAAGACTACTATGATTATATAATTGTCGGAGGTGGAACTGCTGGATGTCCATTGGCAGCTACCTTGTCACAGAATTACAAGGTATTGTTGCTGGAACGAGGAGGAGTACCATATGGGAATTCAAACTTGATGACCCAAGAAGGTTTCTTGTCCACCATTCTGGAGATTGATACATTTAATTCTCCTGCACAAGCATTTACTTCAGAGGAGGGTGTGCCTAATACTCGTGGACGGGTTCTGGGTGGAAGCAGCGTTATAAATGCTGGGTTTTACAGCAGAGCTGATCTAGAGTTTTATGCCAAGTCTGGCCTTAGTTGGGATTTAAGAATGGTCAATCAGTCCTATGAGTGGGTGGAGAATGCAATTGTGTTCCGACCTGATCTAAGAAGTTGGCAATCTTCTGTTAGAGATGGCCTGTTGGAAGCTGGTGTGAGTCCATATAATGATTTCAGTTTGGAGCATGTTTTAGGAACAAAAATTGGTGGATCCACATTTGATAGCTCTGGTAGGAGGCATAGTGCTGCAGATCTTTTGAGCTATGGAACAGCTTCAAATATAAAGGTAGCTGTTCATGCAAATGTAGAGAGGATTATAGTTGCCACATCTTCTGCATCTGACAACTCGAAATTATCAGCAATCGGAGTGGTGTATCGTGATACAATGGGGCATTACCATCATGCAATGGTGCGTGGTGATGGGGAGGTCATTCTTTCAGCTGGTGCTATAGGAAGTCCTCAACTTCTTTTACTTAGTGGAATCGGTTCAAGGCCATATCTTTCATCATGGGGTATTCCAGTTTCCCATCACTCGCCTTATGTGGGCCAGTTTCTTTACGACAACCCAAGAAATGGAATCTCGATTGTACCGCCAATGCCTCTGGAACATTCACTTATTCAAGTTGTGGGCATCACTGATTCAGGAGCTTACCTGGAGGCTGCATCAAATGTAATCCCCTTTGGTGCCCTTTCCCATTCTGTTTTTTCCAGGTCACCAACTTCTCCTCTTTACCTGACAGTGGCCACAATTATGGAGAAAATCTCTGGTCCAGTTTCATCAGGTTCACTTAGACTGGCTTCTACAGATGTTAGGCTCAATCCAAGCATCAGGTTCAACTATTTTAGTGACCCAAAAGATCTGGATTTGTGTATTGTTGGGATGCGTAAAATAGGGGATATTCTAAGAAGCCGGTCCATGGAAGATTTTAAGTTTACATGGGCTGGAGGTAGAGACTTCAGATTTTTAGGACCTGCATTGCCCACTGACCAATCAGATGATGCACTAATGGGTGAATTTTGTCGGCGGACAGTGAGCACTATATGGCATTATCATGGTGGTTGTGTAGTAGGGAAAGTCGTGGATACTGATCTCCGGGTGACTGGTGTCAATGCTCTGAGAGTCATTGATGGTTCAATATTGACAGTATCTCCAGGAACCAATCCTCAGGCAACACTTCTAATGCTTGGAAGGTGATTGGTTTTAAATTAATTAATTAGTCAAGCTTATAGTTTTCAATTCATGTGAGAAATTCCCCTAACTCTAGTGCCTGTCTGTTTTCTTTGCCAGATATATGGGACTGAAGATGCTCCAAGAACGAACAAGATAAGATGTCAGTTGTGATCAAAATTCAAAATTCAAAATTCATAGCAGAGGGTTGGTTTATGATTCTTTTATGCTTGATTTTTTGTGTGTTCATACTTGGTAGATGTATGAGAAATCGTATTGTTAGATGCACTGACAAGTATAGAGGTTATCTGTATATGTTTTAGGATTTGTTCTGTAATGAGCACCATTGTGCTCTTATTTTGGATCATGTGATGGTTATATGGCATACTATCCAAGTATGCATGCATTTACGAATTTTGATCCTACTATTAATAGTTTCTTCTTCAT

mRNA sequence

GGGTCTAAATTTGGATCTTGTCGGACCCGATCCAGATCCGACTCATTGTTATCCGTATCCTTACTTGACTACTTCCATTCCACGCTGAAAAGAAAATGCAGCCCAATAAGCGTTGCGGTGACCATAAATGCAGCCCGATAGTGTCATCTTTACGAGGCAAACTCGAATAAATGTTCAAAAACTATAAACGCGGGTTAATTGCACACAAGACAATACATACACAAACGGGATTGCCAAAATGCAAAGGCTGTTAAGAATACAACATTCTTTTGGTTCATTATTAAAAAATCTTCCTGCAAAACCAGTTTCTAACCGAATTTCTAGCGACACCCCCCTACCCCCTTTCCCCACTTTCTCTCTCCTCCATTTCTCTCTGTCTACAGTGGCTTCTGCAACTCTGTAGCTGACTACTAGCTGGGCATTGGGGATTACCCATTATCTTTTTCTTGATTACCCATTTCTATCTTCTTCAATTAAACACCAAGAAAAGGAGATATTTCAAAAGAAAATGTCGAAATTGGTGATACCCAGATTGTTTAATGACGAATTCTTCAGTAGATTGCTCGTAATTTTGATCTTGCTCACATTCTTTGGGTGTTGCACAGCTCAACCACAAGAGGCTCCTGCCTATCTTCAATTTACTGCAAATGCTACTGAATTCCCTGCGGAAGACTACTATGATTATATAATTGTCGGAGGTGGAACTGCTGGATGTCCATTGGCAGCTACCTTGTCACAGAATTACAAGGTATTGTTGCTGGAACGAGGAGGAGTACCATATGGGAATTCAAACTTGATGACCCAAGAAGGTTTCTTGTCCACCATTCTGGAGATTGATACATTTAATTCTCCTGCACAAGCATTTACTTCAGAGGAGGGTGTGCCTAATACTCGTGGACGGGTTCTGGGTGGAAGCAGCGTTATAAATGCTGGGTTTTACAGCAGAGCTGATCTAGAGTTTTATGCCAAGTCTGGCCTTAGTTGGGATTTAAGAATGGTCAATCAGTCCTATGAGTGGGTGGAGAATGCAATTGTGTTCCGACCTGATCTAAGAAGTTGGCAATCTTCTGTTAGAGATGGCCTGTTGGAAGCTGGTGTGAGTCCATATAATGATTTCAGTTTGGAGCATGTTTTAGGAACAAAAATTGGTGGATCCACATTTGATAGCTCTGGTAGGAGGCATAGTGCTGCAGATCTTTTGAGCTATGGAACAGCTTCAAATATAAAGGTAGCTGTTCATGCAAATGTAGAGAGGATTATAGTTGCCACATCTTCTGCATCTGACAACTCGAAATTATCAGCAATCGGAGTGGTGTATCGTGATACAATGGGGCATTACCATCATGCAATGGTGCGTGGTGATGGGGAGGTCATTCTTTCAGCTGGTGCTATAGGAAGTCCTCAACTTCTTTTACTTAGTGGAATCGGTTCAAGGCCATATCTTTCATCATGGGGTATTCCAGTTTCCCATCACTCGCCTTATGTGGGCCAGTTTCTTTACGACAACCCAAGAAATGGAATCTCGATTGTACCGCCAATGCCTCTGGAACATTCACTTATTCAAGTTGTGGGCATCACTGATTCAGGAGCTTACCTGGAGGCTGCATCAAATGTAATCCCCTTTGGTGCCCTTTCCCATTCTGTTTTTTCCAGGTCACCAACTTCTCCTCTTTACCTGACAGTGGCCACAATTATGGAGAAAATCTCTGGTCCAGTTTCATCAGGTTCACTTAGACTGGCTTCTACAGATGTTAGGCTCAATCCAAGCATCAGGTTCAACTATTTTAGTGACCCAAAAGATCTGGATTTGTGTATTGTTGGGATGCGTAAAATAGGGGATATTCTAAGAAGCCGGTCCATGGAAGATTTTAAGTTTACATGGGCTGGAGGTAGAGACTTCAGATTTTTAGGACCTGCATTGCCCACTGACCAATCAGATGATGCACTAATGGGTGAATTTTGTCGGCGGACAGTGAGCACTATATGGCATTATCATGGTGGTTGTGTAGTAGGGAAAGTCGTGGATACTGATCTCCGGGTGACTGGTGTCAATGCTCTGAGAGTCATTGATGGTTCAATATTGACAGTATCTCCAGGAACCAATCCTCAGGCAACACTTCTAATGCTTGGAAGATATATGGGACTGAAGATGCTCCAAGAACGAACAAGATAAGATGTCAGTTGTGATCAAAATTCAAAATTCAAAATTCATAGCAGAGGGTTGGTTTATGATTCTTTTATGCTTGATTTTTTGTGTGTTCATACTTGGTAGATGTATGAGAAATCGTATTGTTAGATGCACTGACAAGTATAGAGGTTATCTGTATATGTTTTAGGATTTGTTCTGTAATGAGCACCATTGTGCTCTTATTTTGGATCATGTGATGGTTATATGGCATACTATCCAAGTATGCATGCATTTACGAATTTTGATCCTACTATTAATAGTTTCTTCTTCAT

Coding sequence (CDS)

ATGTCGAAATTGGTGATACCCAGATTGTTTAATGACGAATTCTTCAGTAGATTGCTCGTAATTTTGATCTTGCTCACATTCTTTGGGTGTTGCACAGCTCAACCACAAGAGGCTCCTGCCTATCTTCAATTTACTGCAAATGCTACTGAATTCCCTGCGGAAGACTACTATGATTATATAATTGTCGGAGGTGGAACTGCTGGATGTCCATTGGCAGCTACCTTGTCACAGAATTACAAGGTATTGTTGCTGGAACGAGGAGGAGTACCATATGGGAATTCAAACTTGATGACCCAAGAAGGTTTCTTGTCCACCATTCTGGAGATTGATACATTTAATTCTCCTGCACAAGCATTTACTTCAGAGGAGGGTGTGCCTAATACTCGTGGACGGGTTCTGGGTGGAAGCAGCGTTATAAATGCTGGGTTTTACAGCAGAGCTGATCTAGAGTTTTATGCCAAGTCTGGCCTTAGTTGGGATTTAAGAATGGTCAATCAGTCCTATGAGTGGGTGGAGAATGCAATTGTGTTCCGACCTGATCTAAGAAGTTGGCAATCTTCTGTTAGAGATGGCCTGTTGGAAGCTGGTGTGAGTCCATATAATGATTTCAGTTTGGAGCATGTTTTAGGAACAAAAATTGGTGGATCCACATTTGATAGCTCTGGTAGGAGGCATAGTGCTGCAGATCTTTTGAGCTATGGAACAGCTTCAAATATAAAGGTAGCTGTTCATGCAAATGTAGAGAGGATTATAGTTGCCACATCTTCTGCATCTGACAACTCGAAATTATCAGCAATCGGAGTGGTGTATCGTGATACAATGGGGCATTACCATCATGCAATGGTGCGTGGTGATGGGGAGGTCATTCTTTCAGCTGGTGCTATAGGAAGTCCTCAACTTCTTTTACTTAGTGGAATCGGTTCAAGGCCATATCTTTCATCATGGGGTATTCCAGTTTCCCATCACTCGCCTTATGTGGGCCAGTTTCTTTACGACAACCCAAGAAATGGAATCTCGATTGTACCGCCAATGCCTCTGGAACATTCACTTATTCAAGTTGTGGGCATCACTGATTCAGGAGCTTACCTGGAGGCTGCATCAAATGTAATCCCCTTTGGTGCCCTTTCCCATTCTGTTTTTTCCAGGTCACCAACTTCTCCTCTTTACCTGACAGTGGCCACAATTATGGAGAAAATCTCTGGTCCAGTTTCATCAGGTTCACTTAGACTGGCTTCTACAGATGTTAGGCTCAATCCAAGCATCAGGTTCAACTATTTTAGTGACCCAAAAGATCTGGATTTGTGTATTGTTGGGATGCGTAAAATAGGGGATATTCTAAGAAGCCGGTCCATGGAAGATTTTAAGTTTACATGGGCTGGAGGTAGAGACTTCAGATTTTTAGGACCTGCATTGCCCACTGACCAATCAGATGATGCACTAATGGGTGAATTTTGTCGGCGGACAGTGAGCACTATATGGCATTATCATGGTGGTTGTGTAGTAGGGAAAGTCGTGGATACTGATCTCCGGGTGACTGGTGTCAATGCTCTGAGAGTCATTGATGGTTCAATATTGACAGTATCTCCAGGAACCAATCCTCAGGCAACACTTCTAATGCTTGGAAGATATATGGGACTGAAGATGCTCCAAGAACGAACAAGATAA

Protein sequence

MSKLVIPRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERTR
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo04534.1Spo04534.1mRNA


Homology
[Close]
BLAST of Spo04534.1 vs. NCBI nr
Match: gi|902193537|gb|KNA12319.1| (hypothetical protein SOVF_126720 [Spinacia oleracea])

HSP 1 Score: 1109.0 bits (2867), Expect = 0.000e+0
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 1 

		  

Query: 1   MSKLVIPRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYI 60
           MSKLVIPRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYI
Sbjct: 1   MSKLVIPRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYI 60

Query: 61  IVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFT 120
           IVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFT
Sbjct: 61  IVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFT 120

Query: 121 SEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPD 180
           SEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPD
Sbjct: 121 SEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPD 180

Query: 181 LRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIK 240
           LRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIK
Sbjct: 181 LRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIK 240

Query: 241 VAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQL 300
           VAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQL
Sbjct: 241 VAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQL 300

Query: 301 LLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSG 360
           LLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSG
Sbjct: 301 LLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSG 360

Query: 361 AYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPS 420
           AYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPS
Sbjct: 361 AYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPS 420

Query: 421 IRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDAL 480
           IRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDAL
Sbjct: 421 IRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDAL 480

Query: 481 MGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLML 540
           MGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLML
Sbjct: 481 MGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLML 540

Query: 541 GRYMGLKMLQERTR 555
           GRYMGLKMLQERTR
Sbjct: 541 GRYMGLKMLQERTR 554
[Close]
BLAST of Spo04534.1 vs. NCBI nr
Match: gi|731351926|ref|XP_010687278.1| (PREDICTED: (R)-mandelonitrile lyase-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 977.2 bits (2525), Expect = 1.200e-281
Identity = 476/533 (89.31%), Postives = 511/533 (95.87%), Query Frame = 1 

		  

Query: 20  VILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNY 79
           VILILLTFFG  TAQPQ  PAYLQFTANATEFP EDYYDYIIVGGGT+GCPLAATLS+NY
Sbjct: 10  VILILLTFFGSITAQPQTPPAYLQFTANATEFPIEDYYDYIIVGGGTSGCPLAATLSENY 69

Query: 80  KVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVI 139
           KVLLLERGGVPYGN NLMTQEGFL+T+L+IDTF+SPAQAFTSEEGVPNTRGRVLGGSSVI
Sbjct: 70  KVLLLERGGVPYGNPNLMTQEGFLNTLLQIDTFDSPAQAFTSEEGVPNTRGRVLGGSSVI 129

Query: 140 NAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSP 199
           NAGFYSRAD EFY KSGLSWDLRMVNQSYEWVENAIVFRP+LR+WQS+VRDGLLEAGV+P
Sbjct: 130 NAGFYSRADKEFYEKSGLSWDLRMVNQSYEWVENAIVFRPELRNWQSAVRDGLLEAGVNP 189

Query: 200 YNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASD 259
           YNDFSLEHVLGTKIGGSTFDSSGRRHSAADLL++ TASNIKVAVHANV+R+I+ATSSAS 
Sbjct: 190 YNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLNFATASNIKVAVHANVQRVILATSSASG 249

Query: 260 NSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPV 319
           NSKLSAIGVVYRDT+GHYHHAMVR  GEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPV
Sbjct: 250 NSKLSAIGVVYRDTLGHYHHAMVRDHGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPV 309

Query: 320 SHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHSV 379
           SHH PYVGQFLYDNPRNGISIVPP PLEHSLIQVVGITD+GAYLEAASNVIPF ALSHSV
Sbjct: 310 SHHLPYVGQFLYDNPRNGISIVPPTPLEHSLIQVVGITDTGAYLEAASNVIPFSALSHSV 369

Query: 380 FSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDLCIVGM 439
           F+RSP+SP+YLTVATIMEKISGPVSSGSL+LASTDVRLNPSIRFNYF+DP+DL LCIVG+
Sbjct: 370 FTRSPSSPIYLTVATIMEKISGPVSSGSLQLASTDVRLNPSIRFNYFTDPQDLQLCIVGL 429

Query: 440 RKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHGGC 499
           RKI +ILRSR+MEDFKFTWAGGRDFRFLGP LP D SDDALMGE+CRRTVSTIWHYHGGC
Sbjct: 430 RKIAEILRSRAMEDFKFTWAGGRDFRFLGPGLPIDLSDDALMGEYCRRTVSTIWHYHGGC 489

Query: 500 VVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQER 553
           +VGKVVD DLRV G++ALRV+DGS+LTVSPGTNPQAT+LMLGRYMGLK++QER
Sbjct: 490 IVGKVVDPDLRVIGIDALRVVDGSVLTVSPGTNPQATVLMLGRYMGLKLMQER 542
[Close]
BLAST of Spo04534.1 vs. NCBI nr
Match: gi|661897800|emb|CDO97767.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 817.4 bits (2110), Expect = 1.500e-233
Identity = 394/541 (72.83%), Postives = 465/541 (85.95%), Query Frame = 1 

		  

Query: 15  FSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAAT 74
           +S +L  L++  F     +  Q++P+Y+ F  NATE P+EDYYDYIIVGGGTAGCPLAAT
Sbjct: 8   YSPILTFLMVAAFISLPKSLSQQSPSYMGFVFNATEMPSEDYYDYIIVGGGTAGCPLAAT 67

Query: 75  LSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLG 134
           LS+N++VL+LERGGVPYG  NLMTQEGFLST++++D  +SPAQAFTSE+GVPN RGRVLG
Sbjct: 68  LSENFRVLVLERGGVPYGMPNLMTQEGFLSTLVDLDATDSPAQAFTSEDGVPNARGRVLG 127

Query: 135 GSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLE 194
           GSS INAGFYSRAD +F+  S ++WDLR+VNQSY+WVE AIVFRP+LR WQS++RDGLLE
Sbjct: 128 GSSAINAGFYSRADPDFFRNSLINWDLRVVNQSYKWVEKAIVFRPELRIWQSAIRDGLLE 187

Query: 195 AGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVAT 254
           AG+ PYN F+L+HV+GTKIGGSTFDS+GRRHSAADLLSY   SNIKVAV+A+VER++ A+
Sbjct: 188 AGIGPYNGFALDHVVGTKIGGSTFDSAGRRHSAADLLSYAKPSNIKVAVYASVERVLFAS 247

Query: 255 SSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSS 314
           S AS   + SAIGVV+RD  G +HHAM+R  GEV+LSAGAIGSPQLLLLSGIG RPYLSS
Sbjct: 248 SPASAAPRQSAIGVVFRDKTGRFHHAMLREMGEVLLSAGAIGSPQLLLLSGIGPRPYLSS 307

Query: 315 WGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGA 374
           WGIPV HH PYVGQFLYDNPRNGISIVPP+PLE SLIQVVGIT SGAYLEAASN IPF +
Sbjct: 308 WGIPVVHHHPYVGQFLYDNPRNGISIVPPVPLEQSLIQVVGITSSGAYLEAASNAIPFAS 367

Query: 375 LSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDL 434
            +H+ FSR+P SP+Y TVAT+MEKI GP+S+GSLRLASTDVR+NP +RFNYFSDP D + 
Sbjct: 368 RAHNFFSRTPPSPVYFTVATLMEKIVGPLSAGSLRLASTDVRVNPIVRFNYFSDPADTER 427

Query: 435 CIVGMRKIGDILRSRSMEDFKFTWA-GGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIW 494
           C+ G RKI D+LRSRSMEDFKF+   G RDFR++GP LP D S+D LMGEFCRRTV+TIW
Sbjct: 428 CVNGTRKIADVLRSRSMEDFKFSQLFGDRDFRYIGPPLPVDLSNDRLMGEFCRRTVNTIW 487

Query: 495 HYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERT 554
           HYHGGC+VGKVVD + RV G+ ALRV+DGSI TVSPGTNPQATLLMLGRY+G+K+L+ERT
Sbjct: 488 HYHGGCLVGKVVDRNFRVLGIQALRVVDGSIFTVSPGTNPQATLLMLGRYVGMKILRERT 547
[Close]
BLAST of Spo04534.1 vs. NCBI nr
Match: gi|697120115|ref|XP_009614027.1| (PREDICTED: (R)-mandelonitrile lyase-like [Nicotiana tomentosiformis])

HSP 1 Score: 817.4 bits (2110), Expect = 1.500e-233
Identity = 385/519 (74.18%), Postives = 457/519 (88.05%), Query Frame = 1 

		  

Query: 37  EAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNL 96
           E+P Y++F  NATEFP+EDYYDYI+VGGGTAGCPLAATLS+ YKVLLLERGGVPYG  NL
Sbjct: 33  ESPNYMRFVFNATEFPSEDYYDYIVVGGGTAGCPLAATLSEKYKVLLLERGGVPYGKPNL 92

Query: 97  MTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSG 156
           MTQEGFL+ + ++D F SPAQ FTSE+GVPN RGR+LGGSS INAGFYSRAD +FY +SG
Sbjct: 93  MTQEGFLTVLTDVDDFESPAQTFTSEDGVPNARGRILGGSSAINAGFYSRADQDFYVRSG 152

Query: 157 LSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGS 216
           + WDL +VNQSYEWVE +IVFRP+L++WQS+VRDGL+EAG+ P+N FSL+HVLGTKIGGS
Sbjct: 153 IKWDLHVVNQSYEWVERSIVFRPELKNWQSAVRDGLVEAGIDPFNGFSLDHVLGTKIGGS 212

Query: 217 TFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGH 276
           TFDSSGRRHSAAD+L+Y   SNI+VAV+A+VER+++A S+    SK++AIGV++RD  G 
Sbjct: 213 TFDSSGRRHSAADILNYANPSNIQVAVYASVERVLLAQSAPYSPSKMAAIGVIFRDQAGR 272

Query: 277 YHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRN 336
           YHHAMVRG GEV+LSAGA+GSPQLLLLSG+G RPYLS+WGIPV+HH PYVGQFL+DNPRN
Sbjct: 273 YHHAMVRGKGEVLLSAGALGSPQLLLLSGVGPRPYLSTWGIPVAHHLPYVGQFLFDNPRN 332

Query: 337 GISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIM 396
           GISIVPPMPLEHSLIQVVGITD+GAYLEAASNVIPF + ++SVF RS  SP+YLTVAT+M
Sbjct: 333 GISIVPPMPLEHSLIQVVGITDAGAYLEAASNVIPFASPANSVFIRSSPSPVYLTVATLM 392

Query: 397 EKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKF 456
           EKI GPVSSGSLRLASTDVR+NP +RFNYFS   D++ C+ G RKI D+LR+R+M+ FKF
Sbjct: 393 EKIVGPVSSGSLRLASTDVRVNPIVRFNYFSSSGDVERCVNGTRKIADVLRTRTMDIFKF 452

Query: 457 -TWAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVN 516
             W GGRDFR++GPALP D+S+D LM EFC RTVSTIWHYHGGCVVGKVVD +LRV G++
Sbjct: 453 DQWFGGRDFRYVGPALPVDESNDELMREFCHRTVSTIWHYHGGCVVGKVVDQNLRVLGID 512

Query: 517 ALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERTR 555
            LRV+DGS+ +VSPGTNPQATLLMLGRY+G+ ML+ER R
Sbjct: 513 GLRVVDGSVFSVSPGTNPQATLLMLGRYVGMVMLRERFR 551
[Close]
BLAST of Spo04534.1 vs. NCBI nr
Match: gi|225427155|ref|XP_002277531.1| (PREDICTED: (R)-mandelonitrile lyase-like isoform X1 [Vitis vinifera])

HSP 1 Score: 812.4 bits (2097), Expect = 4.900e-232
Identity = 388/537 (72.25%), Postives = 462/537 (86.03%), Query Frame = 1 

		  

Query: 19  LVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQN 78
           L +LI+ +       Q  + P+Y +F  NATE P EDYYDYI+VGGGTAGCPLAATLS+N
Sbjct: 12  LALLIVFSLHHLSRGQFPQGPSYSKFVVNATEMPPEDYYDYIVVGGGTAGCPLAATLSEN 71

Query: 79  YKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSV 138
           ++VL+LERGGVPY N NLM+Q+GFL++++E D ++SPAQ FTSE+GV N RGRVLGGSS 
Sbjct: 72  FRVLVLERGGVPYTNPNLMSQDGFLTSLMEADPYDSPAQTFTSEDGVANARGRVLGGSSA 131

Query: 139 INAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVS 198
           INAGFYSRAD +FY KSGL WDL +VNQSYEWVE A+VFRP+L++WQS+VRDGLLEAGV 
Sbjct: 132 INAGFYSRADEDFYKKSGLKWDLHIVNQSYEWVERAVVFRPELKNWQSAVRDGLLEAGVD 191

Query: 199 PYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSAS 258
           PY  F L+H +GTKIGGSTFDSSGRRH+AADLL Y  A+NI+VAVHA+VERI++A +SA 
Sbjct: 192 PYRGFILDHAVGTKIGGSTFDSSGRRHTAADLLGYAKATNIRVAVHASVERILLAPTSAL 251

Query: 259 DNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIP 318
             SK SAIGVVYRD +G YHHAMVR +GEVILSAGAIG+PQLLLLSGIG R YLSSWGIP
Sbjct: 252 SGSKQSAIGVVYRDRIGRYHHAMVRENGEVILSAGAIGTPQLLLLSGIGPRSYLSSWGIP 311

Query: 319 VSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHS 378
           V HH PYVGQFLYDNPRNGISIV P+PLEHSLIQVVGIT+ GAY+EAASNVIPF + + S
Sbjct: 312 VVHHLPYVGQFLYDNPRNGISIVSPIPLEHSLIQVVGITNLGAYIEAASNVIPFASPARS 371

Query: 379 VFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDLCIVG 438
           VF R+P+SPLYLTVAT+MEKI GP+SSGSL+LASTD+++NP +RFNYF+DP DL+ C+ G
Sbjct: 372 VFIRTPSSPLYLTVATLMEKIIGPLSSGSLQLASTDIKVNPLVRFNYFADPLDLERCVSG 431

Query: 439 MRKIGDILRSRSMEDFKF-TWAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHG 498
           MRK+GD+LRSRSME+FKF  W GG+DFRF+GPALP +QS+DA M EFC RTVSTIWHYHG
Sbjct: 432 MRKVGDVLRSRSMEEFKFREWFGGQDFRFVGPALPVNQSNDAQMAEFCHRTVSTIWHYHG 491

Query: 499 GCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERTR 555
           GC+VGKVVD+D +V G++ALRV+DGS   VSPGTNPQATL+MLGRY+G+K+ +ER R
Sbjct: 492 GCIVGKVVDSDHQVLGIDALRVVDGSTFNVSPGTNPQATLMMLGRYIGIKITKERMR 548
[Close]
BLAST of Spo04534.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QYH0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_126720 PE=3 SV=1)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.000e+0
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 1 

		  

Query: 1   MSKLVIPRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYI 60
           MSKLVIPRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYI
Sbjct: 1   MSKLVIPRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYI 60

Query: 61  IVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFT 120
           IVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFT
Sbjct: 61  IVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFT 120

Query: 121 SEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPD 180
           SEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPD
Sbjct: 121 SEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPD 180

Query: 181 LRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIK 240
           LRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIK
Sbjct: 181 LRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIK 240

Query: 241 VAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQL 300
           VAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQL
Sbjct: 241 VAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQL 300

Query: 301 LLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSG 360
           LLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSG
Sbjct: 301 LLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSG 360

Query: 361 AYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPS 420
           AYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPS
Sbjct: 361 AYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPS 420

Query: 421 IRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDAL 480
           IRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDAL
Sbjct: 421 IRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDAL 480

Query: 481 MGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLML 540
           MGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLML
Sbjct: 481 MGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLML 540

Query: 541 GRYMGLKMLQERTR 555
           GRYMGLKMLQERTR
Sbjct: 541 GRYMGLKMLQERTR 554
[Close]
BLAST of Spo04534.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BV03_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g189010 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 8.100e-282
Identity = 476/533 (89.31%), Postives = 511/533 (95.87%), Query Frame = 1 

		  

Query: 20  VILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNY 79
           VILILLTFFG  TAQPQ  PAYLQFTANATEFP EDYYDYIIVGGGT+GCPLAATLS+NY
Sbjct: 10  VILILLTFFGSITAQPQTPPAYLQFTANATEFPIEDYYDYIIVGGGTSGCPLAATLSENY 69

Query: 80  KVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVI 139
           KVLLLERGGVPYGN NLMTQEGFL+T+L+IDTF+SPAQAFTSEEGVPNTRGRVLGGSSVI
Sbjct: 70  KVLLLERGGVPYGNPNLMTQEGFLNTLLQIDTFDSPAQAFTSEEGVPNTRGRVLGGSSVI 129

Query: 140 NAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSP 199
           NAGFYSRAD EFY KSGLSWDLRMVNQSYEWVENAIVFRP+LR+WQS+VRDGLLEAGV+P
Sbjct: 130 NAGFYSRADKEFYEKSGLSWDLRMVNQSYEWVENAIVFRPELRNWQSAVRDGLLEAGVNP 189

Query: 200 YNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASD 259
           YNDFSLEHVLGTKIGGSTFDSSGRRHSAADLL++ TASNIKVAVHANV+R+I+ATSSAS 
Sbjct: 190 YNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLNFATASNIKVAVHANVQRVILATSSASG 249

Query: 260 NSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPV 319
           NSKLSAIGVVYRDT+GHYHHAMVR  GEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPV
Sbjct: 250 NSKLSAIGVVYRDTLGHYHHAMVRDHGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPV 309

Query: 320 SHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHSV 379
           SHH PYVGQFLYDNPRNGISIVPP PLEHSLIQVVGITD+GAYLEAASNVIPF ALSHSV
Sbjct: 310 SHHLPYVGQFLYDNPRNGISIVPPTPLEHSLIQVVGITDTGAYLEAASNVIPFSALSHSV 369

Query: 380 FSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDLCIVGM 439
           F+RSP+SP+YLTVATIMEKISGPVSSGSL+LASTDVRLNPSIRFNYF+DP+DL LCIVG+
Sbjct: 370 FTRSPSSPIYLTVATIMEKISGPVSSGSLQLASTDVRLNPSIRFNYFTDPQDLQLCIVGL 429

Query: 440 RKIGDILRSRSMEDFKFTWAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHGGC 499
           RKI +ILRSR+MEDFKFTWAGGRDFRFLGP LP D SDDALMGE+CRRTVSTIWHYHGGC
Sbjct: 430 RKIAEILRSRAMEDFKFTWAGGRDFRFLGPGLPIDLSDDALMGEYCRRTVSTIWHYHGGC 489

Query: 500 VVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQER 553
           +VGKVVD DLRV G++ALRV+DGS+LTVSPGTNPQAT+LMLGRYMGLK++QER
Sbjct: 490 IVGKVVDPDLRVIGIDALRVVDGSVLTVSPGTNPQATVLMLGRYMGLKLMQER 542
[Close]
BLAST of Spo04534.1 vs. UniProtKB/TrEMBL
Match: A0A068TP29_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00021661001 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.100e-233
Identity = 394/541 (72.83%), Postives = 465/541 (85.95%), Query Frame = 1 

		  

Query: 15  FSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAAT 74
           +S +L  L++  F     +  Q++P+Y+ F  NATE P+EDYYDYIIVGGGTAGCPLAAT
Sbjct: 8   YSPILTFLMVAAFISLPKSLSQQSPSYMGFVFNATEMPSEDYYDYIIVGGGTAGCPLAAT 67

Query: 75  LSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLG 134
           LS+N++VL+LERGGVPYG  NLMTQEGFLST++++D  +SPAQAFTSE+GVPN RGRVLG
Sbjct: 68  LSENFRVLVLERGGVPYGMPNLMTQEGFLSTLVDLDATDSPAQAFTSEDGVPNARGRVLG 127

Query: 135 GSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLE 194
           GSS INAGFYSRAD +F+  S ++WDLR+VNQSY+WVE AIVFRP+LR WQS++RDGLLE
Sbjct: 128 GSSAINAGFYSRADPDFFRNSLINWDLRVVNQSYKWVEKAIVFRPELRIWQSAIRDGLLE 187

Query: 195 AGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVAT 254
           AG+ PYN F+L+HV+GTKIGGSTFDS+GRRHSAADLLSY   SNIKVAV+A+VER++ A+
Sbjct: 188 AGIGPYNGFALDHVVGTKIGGSTFDSAGRRHSAADLLSYAKPSNIKVAVYASVERVLFAS 247

Query: 255 SSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSS 314
           S AS   + SAIGVV+RD  G +HHAM+R  GEV+LSAGAIGSPQLLLLSGIG RPYLSS
Sbjct: 248 SPASAAPRQSAIGVVFRDKTGRFHHAMLREMGEVLLSAGAIGSPQLLLLSGIGPRPYLSS 307

Query: 315 WGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGA 374
           WGIPV HH PYVGQFLYDNPRNGISIVPP+PLE SLIQVVGIT SGAYLEAASN IPF +
Sbjct: 308 WGIPVVHHHPYVGQFLYDNPRNGISIVPPVPLEQSLIQVVGITSSGAYLEAASNAIPFAS 367

Query: 375 LSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDL 434
            +H+ FSR+P SP+Y TVAT+MEKI GP+S+GSLRLASTDVR+NP +RFNYFSDP D + 
Sbjct: 368 RAHNFFSRTPPSPVYFTVATLMEKIVGPLSAGSLRLASTDVRVNPIVRFNYFSDPADTER 427

Query: 435 CIVGMRKIGDILRSRSMEDFKFTWA-GGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIW 494
           C+ G RKI D+LRSRSMEDFKF+   G RDFR++GP LP D S+D LMGEFCRRTV+TIW
Sbjct: 428 CVNGTRKIADVLRSRSMEDFKFSQLFGDRDFRYIGPPLPVDLSNDRLMGEFCRRTVNTIW 487

Query: 495 HYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERT 554
           HYHGGC+VGKVVD + RV G+ ALRV+DGSI TVSPGTNPQATLLMLGRY+G+K+L+ERT
Sbjct: 488 HYHGGCLVGKVVDRNFRVLGIQALRVVDGSIFTVSPGTNPQATLLMLGRYVGMKILRERT 547
[Close]
BLAST of Spo04534.1 vs. UniProtKB/TrEMBL
Match: V4S0T4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10025302mg PE=3 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 1.900e-230
Identity = 384/548 (70.07%), Postives = 468/548 (85.40%), Query Frame = 1 

		  

Query: 7   PRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGT 66
           P  F+ ++ + L+++  ++++    + +  + P+YL+F  NAT+FP+EDYYDY++VGGGT
Sbjct: 6   PICFSLQYIAFLVLVCSVVSWARPHSQRQTQEPSYLKFVHNATDFPSEDYYDYVVVGGGT 65

Query: 67  AGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVP 126
           AGCPLAATLSQ++KVLLLERGG  YG  NLMTQEGFL+ ++E DTF+SPAQ FTSE+GVP
Sbjct: 66  AGCPLAATLSQSFKVLLLERGGTAYGKPNLMTQEGFLTALMETDTFDSPAQGFTSEDGVP 125

Query: 127 NTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQS 186
           N RGRVLGGSS INAGFYSRAD +FY +SG++WDL++VN+SYEWVE A+VFRP LR+WQS
Sbjct: 126 NARGRVLGGSSAINAGFYSRADQDFYRRSGMNWDLKVVNESYEWVEEAVVFRPQLRNWQS 185

Query: 187 SVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHAN 246
           +VRDGLLEAGV PYN F ++HV+GTKIGG+TFDSSGRRHSAADLLSY  A NI+VA++A+
Sbjct: 186 AVRDGLLEAGVDPYNGFRVDHVVGTKIGGTTFDSSGRRHSAADLLSYANARNIRVAIYAS 245

Query: 247 VERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGI 306
           VER+++A SS    S  +AIGVVYRD  G YHHAMVR  GEVIL AGAIGSPQLL LSGI
Sbjct: 246 VERVLLAASSPYPGSGQAAIGVVYRDRTGRYHHAMVREKGEVILCAGAIGSPQLLFLSGI 305

Query: 307 GSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAA 366
           G+RPYLSS GIPV++H PYVGQ++YDNPRNGISIVPP+PLEHSLIQVVGIT+ GAY+EAA
Sbjct: 306 GARPYLSSLGIPVAYHHPYVGQYMYDNPRNGISIVPPIPLEHSLIQVVGITEIGAYVEAA 365

Query: 367 SNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYF 426
           S VIPF +L  SVF R+P+ PLYLTVAT+MEKI GP+SSGSLRLASTDVR+NP +RFNYF
Sbjct: 366 STVIPFASLPRSVFIRTPSPPLYLTVATLMEKIIGPLSSGSLRLASTDVRVNPIVRFNYF 425

Query: 427 SDPKDLDLCIVGMRKIGDILRSRSMEDFKF-TWAGGRDFRFLGPALPTDQSDDALMGEFC 486
           S+P D++ CI G RKIGD+LRSRSM+DF+   W G R+FRF+GPALP DQSD A M EFC
Sbjct: 426 SNPVDVERCINGTRKIGDVLRSRSMDDFRLHEWFGARNFRFVGPALPVDQSDHAQMAEFC 485

Query: 487 RRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMG 546
           RRTVSTIWHYHGGCVVGKVVD DLRV G++ALR++DGS  ++SPGTNPQATL+MLGRY+G
Sbjct: 486 RRTVSTIWHYHGGCVVGKVVDRDLRVIGIDALRIVDGSTFSISPGTNPQATLMMLGRYVG 545

Query: 547 LKMLQERT 554
            K+++ R+
Sbjct: 546 AKLIRYRS 553
[Close]
BLAST of Spo04534.1 vs. UniProtKB/TrEMBL
Match: A0A067DRK1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g008715mg PE=3 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 3.200e-230
Identity = 384/548 (70.07%), Postives = 468/548 (85.40%), Query Frame = 1 

		  

Query: 7   PRLFNDEFFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGT 66
           P  F+ ++ + L+++  ++++    + +  + P+YL+F  NAT+FP+EDYYDY++VGGGT
Sbjct: 6   PICFSLQYIAFLVLVCSVVSWAIPHSQRQTQEPSYLKFVHNATDFPSEDYYDYVVVGGGT 65

Query: 67  AGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVP 126
           AGCPLAATLSQ++KVLLLERGG  YG  NLMTQEGFL+ ++E DTF+SPAQ FTSE+GVP
Sbjct: 66  AGCPLAATLSQSFKVLLLERGGTAYGKPNLMTQEGFLTALMETDTFDSPAQGFTSEDGVP 125

Query: 127 NTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQS 186
           N RGRVLGGSS INAGFYSRAD +FY +SG++WDL++VN+SYEWVE A+VFRP LR+WQS
Sbjct: 126 NARGRVLGGSSAINAGFYSRADQDFYRRSGMNWDLKVVNESYEWVEEAVVFRPQLRNWQS 185

Query: 187 SVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHAN 246
           +VRDGLLEAGV PYN F ++HV+GTKIGG+TFDSSGRRHSAADLLSY  A NI+VA++A+
Sbjct: 186 AVRDGLLEAGVDPYNGFRVDHVVGTKIGGTTFDSSGRRHSAADLLSYANARNIRVAIYAS 245

Query: 247 VERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGI 306
           VER+++A SS    S  +AIGVVYRD  G YHHAMVR  GEVIL AGAIGSPQLL LSGI
Sbjct: 246 VERVLLAASSPYPGSGQAAIGVVYRDRTGRYHHAMVREKGEVILCAGAIGSPQLLFLSGI 305

Query: 307 GSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAA 366
           G+RPYLSS GIPV++H PYVGQ++YDNPRNGISIVPP+PLEHSLIQVVGIT+ GAY+EAA
Sbjct: 306 GARPYLSSLGIPVAYHHPYVGQYMYDNPRNGISIVPPIPLEHSLIQVVGITEIGAYVEAA 365

Query: 367 SNVIPFGALSHSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYF 426
           S VIPF +L  SVF R+P+ PLYLTVAT+MEKI GP+SSGSLRLASTDVR+NP +RFNYF
Sbjct: 366 STVIPFASLPRSVFIRTPSPPLYLTVATLMEKIIGPLSSGSLRLASTDVRVNPIVRFNYF 425

Query: 427 SDPKDLDLCIVGMRKIGDILRSRSMEDFKF-TWAGGRDFRFLGPALPTDQSDDALMGEFC 486
           S+P D++ CI G RKIGD+LRSRSM+DF+   W G R+FRF+GPALP DQSD A M EFC
Sbjct: 426 SNPVDVERCINGTRKIGDVLRSRSMDDFRLHEWFGARNFRFVGPALPVDQSDHAQMAEFC 485

Query: 487 RRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMG 546
           RRTVSTIWHYHGGCVVGKVVD DLRV G++ALR++DGS  ++SPGTNPQATL+MLGRY+G
Sbjct: 486 RRTVSTIWHYHGGCVVGKVVDRDLRVIGIDALRIVDGSTFSISPGTNPQATLMMLGRYVG 545

Query: 547 LKMLQERT 554
            K+++ R+
Sbjct: 546 AKLIRYRS 553
[Close]
BLAST of Spo04534.1 vs. ExPASy Swiss-Prot
Match: MDLL_ARATH ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 1.900e-220
Identity = 368/539 (68.27%), Postives = 449/539 (83.30%), Query Frame = 1 

		  

Query: 19  LVILILLTFFGCCTAQPQ--EAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLS 78
           LV+L+LL       A+P+    P +++F +NAT+F +EDYYDYIIVGGGTAGCPLAATLS
Sbjct: 14  LVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLS 73

Query: 79  QNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGS 138
           Q+++VLLLERGGVPY   N+M+ +GFL+T+ +++ F+SPAQ+F SEEGVPN RGRVLGGS
Sbjct: 74  QSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGS 133

Query: 139 SVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAG 198
           S INAGFYSRAD +F+  SGL WDL  VNQSYEWVE AIVFRP LR+WQ+++RD LLE G
Sbjct: 134 SAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVG 193

Query: 199 VSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSS 258
           V P+N F+LEH +GTKIGGSTFD +GRRHS+ADLL Y  +SNI+VAV+A VER+++A+S 
Sbjct: 194 VHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSP 253

Query: 259 ASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWG 318
           +   S +SAIGVVYRD +G +HHA++R  GEVILSAGA+GSPQLL LSGIG R YLS+WG
Sbjct: 254 SVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWG 313

Query: 319 IPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALS 378
           IPV+   P+VG F+YDNPRNGISIVPP+P+E+SLIQVVG+T+ GA+LEAASNVIPF +  
Sbjct: 314 IPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPL 373

Query: 379 HSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDLCI 438
           HSVF R+P SPLY+ V TIMEKI GPVS G LRLASTDVR+NP +RFNYFSDP+DL+ C+
Sbjct: 374 HSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCV 433

Query: 439 VGMRKIGDILRSRSMEDFKF-TWAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHY 498
            G RKIG+ILRSR+M+DF    W G R FRF+G  LP DQS+D +M +FCRRTVSTIWHY
Sbjct: 434 NGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHY 493

Query: 499 HGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERTR 555
           HGG VVGKVVD+DL+V GVN+LR++DGS   +SPGTNPQATL+MLGRYMGLKML+ER R
Sbjct: 494 HGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERMR 552
[Close]
BLAST of Spo04534.1 vs. ExPASy Swiss-Prot
Match: MDL1_PRUSE ((R)-mandelonitrile lyase 1 OS=Prunus serotina GN=MDL1 PE=1 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.300e-147
Identity = 257/516 (49.81%), Postives = 365/516 (70.74%), Query Frame = 1 

		  

Query: 40  AYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQ 99
           +YL+F  +AT+   E  YDY+IVGGGT+GCPLAATLS+ YKVL+LERG +P    N++T 
Sbjct: 37  SYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTA 96

Query: 100 EGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSW 159
           +GF+  + + D   +P + F SE+G+ N RGRVLGG+S+INAG Y+RA+   Y+ SG+ W
Sbjct: 97  DGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDW 156

Query: 160 DLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFD 219
           D+ +VN++YEWVE+ IVF+P+ + WQS      LEAGV P + FSL+H  GT+I GSTFD
Sbjct: 157 DMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFD 216

Query: 220 SSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHH 279
           + G RH+A +LL+ G ++N++V VHA+VE+II + +       L+A GV+YRD+ G  H 
Sbjct: 217 NKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPG-----LTATGVIYRDSNGTPHR 276

Query: 280 AMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGIS 339
           A VR  GEVI+SAG IG+PQLLLLSG+G   YLSS  IPV    PYVGQFL+DNPRN I+
Sbjct: 277 AFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFIN 336

Query: 340 IVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKI 399
           I+PP P+E +++ V+GI++   + + + + +PF     S F  +       T A    K+
Sbjct: 337 ILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPPFSFFPSTSYPLPNSTFAHFASKV 396

Query: 400 SGPVSSGSLRL-ASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKF-T 459
           +GP+S GSL L +S++VR++P+++FNY+S+P DL  C+ GM+KIG++L + +++ +K   
Sbjct: 397 AGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVED 456

Query: 460 WAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNAL 519
             G   F  LG  LP DQ+DDA    FCR +V++ WHYHGGC+VGKV+D D RVTG++AL
Sbjct: 457 LPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDAL 516

Query: 520 RVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERT 554
           RV+DGS    +P ++PQ   LMLGRY+G+K+LQER+
Sbjct: 517 RVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERS 545
[Close]
BLAST of Spo04534.1 vs. ExPASy Swiss-Prot
Match: MDL2_PRUDU ((R)-mandelonitrile lyase 2 OS=Prunus dulcis GN=MDL2 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.700e-147
Identity = 257/516 (49.81%), Postives = 364/516 (70.54%), Query Frame = 1 

		  

Query: 40  AYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQ 99
           +YL F  +AT+   E  YDY+IVGGGT+GCPLAATLS+ YKVL+LERG +P    N++T 
Sbjct: 37  SYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTA 96

Query: 100 EGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSW 159
           +GF+  + + D   +P + F SE+G+ N RGRVLGG+S+INAG Y+RA+   Y+ SG+ W
Sbjct: 97  DGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDW 156

Query: 160 DLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFD 219
           D+ +VNQ+YEWVE+ IV++P+ +SWQS  +   LEAGV P + FSL+H  GT+I GSTFD
Sbjct: 157 DMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFD 216

Query: 220 SSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHH 279
           + G RH+A +LL+ G ++N++V VHA+VE+II + +       L+A GV+YRD+ G  H 
Sbjct: 217 NKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPG-----LTATGVIYRDSNGTPHQ 276

Query: 280 AMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGIS 339
           A VR  GEVI+SAG IG+PQLLLLSG+G   YLSS  IPV    PYVGQFL+DNPRN I+
Sbjct: 277 AFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFIN 336

Query: 340 IVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKI 399
           I+PP P+E +++ V+GI++   + + + + +PF       F  +       T A    K+
Sbjct: 337 ILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPPFGFFPSASYPLPNSTFAHFASKV 396

Query: 400 SGPVSSGSLRL-ASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKF-T 459
           +GP+S GSL L +S++VR++P+++FNY+S+  DL  C+ GM+KIG++L + +++ +K   
Sbjct: 397 AGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVED 456

Query: 460 WAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNAL 519
             G   F  LG  LP DQ+DDA    FCR +V++ WHYHGGC+VGKV+D D RVTG+NAL
Sbjct: 457 LPGVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINAL 516

Query: 520 RVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERT 554
           RV+DGS    +P ++PQ   LMLGRY+G+K+LQER+
Sbjct: 517 RVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERS 545
[Close]
BLAST of Spo04534.1 vs. ExPASy Swiss-Prot
Match: MDL3_PRUSE ((R)-mandelonitrile lyase 3 OS=Prunus serotina GN=MDL3 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.100e-146
Identity = 258/516 (50.00%), Postives = 363/516 (70.35%), Query Frame = 1 

		  

Query: 40  AYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQ 99
           +YL F  +AT+   E  YDYIIVGGGTAGCPLAATLS NY VL+LERG +P    NL+  
Sbjct: 37  SYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLIS 96

Query: 100 EGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSW 159
           +GF+  + + D   +P + F SE+G+ N RGRVLGG+S+INAG Y RA+  F+ ++G+ W
Sbjct: 97  DGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEW 156

Query: 160 DLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFD 219
           D+ +VNQ+YEWVE+ IVF PD ++WQ+ +    LEAG+ P N FS++H+ GT++ GSTFD
Sbjct: 157 DMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFD 216

Query: 220 SSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHH 279
           ++G RH++ +LL+ G  +N++VAV A VE+II     +S+ S ++AIGV+Y D+ G  H 
Sbjct: 217 NNGTRHASDELLNKGDPNNLRVAVQAAVEKIIF----SSNTSGVTAIGVIYTDSNGTTHQ 276

Query: 280 AMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGIS 339
           A VRG+GEVILSAG IGSPQLLLLSG+G   YL+S  I V    PYVGQ++YDNPRN I+
Sbjct: 277 AFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFIN 336

Query: 340 IVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHSVFSRSPTSPLYLTVATIMEKI 399
           I+PP P+E S + V+GIT    + + + + +PF     S F  +       T A I+ K+
Sbjct: 337 ILPPNPIEASTVTVLGITSD--FYQCSISSLPFDTPPFSFFPTTSYPLPNQTFAHIVNKV 396

Query: 400 SGPVSSGSLRL-ASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTW 459
            GP+S G++ L +S+DVR+ P+++FNY+S+  DL  C+ GM+K+G++L + ++E +K   
Sbjct: 397 PGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVED 456

Query: 460 AGGRD-FRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNAL 519
             G D F  LG  LP +Q+DDA    FCR +V++ WHYHGGC+VGKV+D   RVTG+NAL
Sbjct: 457 LPGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINAL 516

Query: 520 RVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERT 554
           RV+DGS    +P ++PQ   LMLGRYMG+++LQER+
Sbjct: 517 RVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERS 546
[Close]
BLAST of Spo04534.1 vs. ExPASy Swiss-Prot
Match: MDL2_PRUSE ((R)-mandelonitrile lyase 2 OS=Prunus serotina GN=MDL2 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.400e-146
Identity = 263/516 (50.97%), Postives = 360/516 (69.77%), Query Frame = 1 

		  

Query: 40  AYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQ 99
           +YL+F  +A +   E  YDYIIVGGGTAGCPLAATLS NY VL+LERG +P    NL+T 
Sbjct: 38  SYLEFVYDANDTELEGTYDYIIVGGGTAGCPLAATLSANYSVLVLERGTLPTEYPNLLTS 97

Query: 100 EGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSW 159
           +GF+  + + D   +P + F S +G+ N RGRVLGG+S+INAG Y RA+  F+ ++G+ W
Sbjct: 98  DGFIYNLQQEDDGQTPVERFVSGDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEW 157

Query: 160 DLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFD 219
           D+ +VN++Y+WVE+ IVF+PD + WQ+      LE G+ P N FSL+H+ GT++ GSTFD
Sbjct: 158 DMDLVNKTYDWVEDTIVFKPDFQFWQNLTGTAFLEVGILPDNGFSLDHLEGTRLTGSTFD 217

Query: 220 SSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHH 279
           ++G RH++ +LL+ G  +N++VAVHA VE+II     +SD+S ++AIGV+Y D+ G  H 
Sbjct: 218 NNGTRHASDELLNKGDPNNLRVAVHAAVEKIIF----SSDSSGVTAIGVIYTDSNGTTHQ 277

Query: 280 AMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGIS 339
           A VRGDGEVILSAG IGSPQLLLLSG+G   YL+S  I V    PYVGQ++YDNPRN I+
Sbjct: 278 AFVRGDGEVILSAGPIGSPQLLLLSGVGLESYLTSLNISVVASHPYVGQYIYDNPRNFIN 337

Query: 340 IVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHSVFSRSPTSPL-YLTVATIMEK 399
           I+PP P+E S + V+GIT    + + + + +PF       F  +PT PL   T A I+ K
Sbjct: 338 ILPPNPIEASTVTVLGITSD--FYQCSISSLPFSTAPFGFFP-NPTYPLPNTTFAHIVNK 397

Query: 400 ISGPVSSGSLRLAST-DVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKF- 459
           + GP+S G++ L ST DVR+ P++ FNY+S+  DL  C+ GM+KIG+ L S +++ +K  
Sbjct: 398 VPGPLSHGTVLLQSTSDVRVAPNVTFNYYSNTTDLAHCVSGMKKIGEFLSSDALKPYKVE 457

Query: 460 TWAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNA 519
              G   F  LG  LP +Q+DDA    FCR  V++ WHYHGGC+VG+V+D D RVTG+NA
Sbjct: 458 DLPGIEGFDILGIPLPENQTDDAAFETFCREAVASYWHYHGGCLVGEVLDDDFRVTGINA 517

Query: 520 LRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQER 553
           LRV+DGS    +P ++PQ   LMLGRYMG K+LQER
Sbjct: 518 LRVVDGSTFPSTPASHPQGFYLMLGRYMGTKILQER 546
[Close]
BLAST of Spo04534.1 vs. TAIR (Arabidopsis)
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 766.5 bits (1978), Expect = 1.100e-221
Identity = 368/539 (68.27%), Postives = 449/539 (83.30%), Query Frame = 1 

		  

Query: 19  LVILILLTFFGCCTAQPQ--EAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLS 78
           LV+L+LL       A+P+    P +++F +NAT+F +EDYYDYIIVGGGTAGCPLAATLS
Sbjct: 14  LVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLS 73

Query: 79  QNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGS 138
           Q+++VLLLERGGVPY   N+M+ +GFL+T+ +++ F+SPAQ+F SEEGVPN RGRVLGGS
Sbjct: 74  QSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGS 133

Query: 139 SVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAG 198
           S INAGFYSRAD +F+  SGL WDL  VNQSYEWVE AIVFRP LR+WQ+++RD LLE G
Sbjct: 134 SAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVG 193

Query: 199 VSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSS 258
           V P+N F+LEH +GTKIGGSTFD +GRRHS+ADLL Y  +SNI+VAV+A VER+++A+S 
Sbjct: 194 VHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSP 253

Query: 259 ASDNSKLSAIGVVYRDTMGHYHHAMVRGDGEVILSAGAIGSPQLLLLSGIGSRPYLSSWG 318
           +   S +SAIGVVYRD +G +HHA++R  GEVILSAGA+GSPQLL LSGIG R YLS+WG
Sbjct: 254 SVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWG 313

Query: 319 IPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALS 378
           IPV+   P+VG F+YDNPRNGISIVPP+P+E+SLIQVVG+T+ GA+LEAASNVIPF +  
Sbjct: 314 IPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPL 373

Query: 379 HSVFSRSPTSPLYLTVATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDLCI 438
           HSVF R+P SPLY+ V TIMEKI GPVS G LRLASTDVR+NP +RFNYFSDP+DL+ C+
Sbjct: 374 HSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCV 433

Query: 439 VGMRKIGDILRSRSMEDFKF-TWAGGRDFRFLGPALPTDQSDDALMGEFCRRTVSTIWHY 498
            G RKIG+ILRSR+M+DF    W G R FRF+G  LP DQS+D +M +FCRRTVSTIWHY
Sbjct: 434 NGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHY 493

Query: 499 HGGCVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQERTR 555
           HGG VVGKVVD+DL+V GVN+LR++DGS   +SPGTNPQATL+MLGRYMGLKML+ER R
Sbjct: 494 HGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERMR 552
[Close]
BLAST of Spo04534.1 vs. TAIR (Arabidopsis)
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 456.4 bits (1173), Expect = 2.400e-128
Identity = 256/576 (44.44%), Postives = 341/576 (59.20%), Query Frame = 1 

		  

Query: 12  DEFFS-RLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCP 71
           D F+S RL V L L      C++   +AP Y  F  +AT  P   YYDYII+GGGTAGCP
Sbjct: 3   DRFWSWRLFVALSLFLHSPICSSD--KAPNY-SFMRDATGSPTTSYYDYIIIGGGTAGCP 62

Query: 72  LAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRG 131
           LAATLSQN  VLLLERG  PY N N+     F + + ++   +SP+Q F SE+GV N R 
Sbjct: 63  LAATLSQNASVLLLERGDSPYNNPNITRLSAFGAALSDLSE-SSPSQRFVSEDGVINARA 122

Query: 132 RVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRD 191
           RVLGG S +NAGFY+RA  ++    G  WD  + N+SY+WVE  + F+P +  WQ++VRD
Sbjct: 123 RVLGGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRD 182

Query: 192 GLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERI 251
           GLLEAG+ P N F+ +H+ GTK GG+ FD +G RH+AADLL Y     I V +HA V RI
Sbjct: 183 GLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRI 242

Query: 252 IVATSSASDNSKLSAIGVVYRDTMGHYHHAMVRGD--GEVILSAGAIGSPQLLLLSGIGS 311
           +  T      +K  A GVVYRD  G  H A ++     E+ILSAG +GSPQLL+LSG+G 
Sbjct: 243 LFRTRG---TTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGP 302

Query: 312 RPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASN 371
              L +  I V    P+VGQ +YDNP N + +  P+P+E SLI+VVGIT  G Y+EAA  
Sbjct: 303 SAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGG 362

Query: 372 VIPFGALSHSVFSRSPTSPLYLTVAT-----------------------IMEKISGPVSS 431
              FG         S T   Y   +                        ++EK+ GP+S+
Sbjct: 363 E-NFGGGGGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLST 422

Query: 432 GSLRLASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFR 491
           G L L + + + NP + FNYF  P DL  C+ G++ I  +++S++   +K+         
Sbjct: 423 GHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVSFEYLL 482

Query: 492 FLGPALPTD---------QSDDALMGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNA 551
            L  + P +          S      EFC+ TV+TIWHYHGGCVVG+VVD D +V G++ 
Sbjct: 483 NLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDR 542

Query: 552 LRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQER 553
           LRVID S +   PGTNPQAT++MLGRYMG+K+L+ER
Sbjct: 543 LRVIDMSTVGYCPGTNPQATVMMLGRYMGVKILRER 568
[Close]
BLAST of Spo04534.1 vs. TAIR (Arabidopsis)
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 448.0 bits (1151), Expect = 8.600e-126
Identity = 263/571 (46.06%), Postives = 355/571 (62.17%), Query Frame = 1 

		  

Query: 14  FFSRLLVILILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAA 73
           FF  +LV + +  F   C +   EA  Y +F  +AT  P   ++DYII+GGGTAGC LAA
Sbjct: 8   FFQFILVAVFI--FHDSCYSD--EAGNY-RFMKDATLAPKLSHFDYIIIGGGTAGCALAA 67

Query: 74  TLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVL 133
           TLSQN  VL+LERGG PY +        F +T+L I T NS +Q F SE+GV N+R RVL
Sbjct: 68  TLSQNATVLVLERGGSPYDDPAATDIGNFANTLLNI-TPNSWSQLFISEDGVFNSRARVL 127

Query: 134 GGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLL 193
           GG +VINAGFYSRA+ +F A++G  W+   V  +YEWVE  +VF P +  WQS+ RDGLL
Sbjct: 128 GGGTVINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLL 187

Query: 194 EAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVA 253
           EAGV+PYN F+ EH++GTK GG+ FD  G RH+AA+LL Y   + I V +HA+V +I+  
Sbjct: 188 EAGVTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKILF- 247

Query: 254 TSSASDNSKLSAIGVVYRDTMG-HYHHAMVRGD---GEVILSAGAIGSPQLLLLSGIGSR 313
             +   N +  A GV++ D  G  Y   +   D    EVILSAGAI SPQLL+LSG+G  
Sbjct: 248 --TIKGNQRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGPA 307

Query: 314 PYLSSWGI-PVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASN 373
            +L+++ + PV    P VGQ + DNP N + I  P P+E SL+Q VGIT  G+Y+E  S 
Sbjct: 308 AHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSA 367

Query: 374 VIPFGALSHSVF---------SRSPTS---------PLYLTVAT----IMEKISGPVSSG 433
           +    +L+ S F         ++ PT           L L V T    I++K++GP+S G
Sbjct: 368 LSLSISLTRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRG 427

Query: 434 SLRLASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFRF 493
            L L +T+   NPS+ FNYF DP+DL+ C+ G+  I  ++ S+    +K+  A  R    
Sbjct: 428 HLELRNTNPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYKYPLASARGLLN 487

Query: 494 LGPALPTDQSDDAL-----MGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVID 553
           L  ALPT+     +     + ++C  TV TI+HYHGGC VGKVVD + +V GV+ALR+ID
Sbjct: 488 LILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLGVDALRIID 547
[Close]
BLAST of Spo04534.1 vs. TAIR (Arabidopsis)
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 446.8 bits (1148), Expect = 1.900e-125
Identity = 255/572 (44.58%), Postives = 343/572 (59.97%), Query Frame = 1 

		  

Query: 22  LILLTFFGCCTAQPQEAPAYLQFTANATEFPAEDYYDYIIVGGGTAGCPLAATLSQNYKV 81
           + ++T+    +  P +A  Y  F  +AT  P    +DYII+GGGT+GC LAATLSQN  V
Sbjct: 12  IFIITYIFHASCSPDKA-GYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASV 71

Query: 82  LLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPAQAFTSEEGVPNTRGRVLGGSSVINA 141
           L+LERGG PY N      E F +T+       S +Q F SE+GV NTR RVLGG SV+NA
Sbjct: 72  LVLERGGAPYDNPTATDIENFATTLSNTSP-KSWSQLFISEDGVYNTRARVLGGGSVLNA 131

Query: 142 GFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIVFRPDLRSWQSSVRDGLLEAGVSPYN 201
           GFY+RA  E+  ++   W    V  +YEWVE  + F+P +  WQ++ +DGLLEAG  PYN
Sbjct: 132 GFYTRAGDEYVKET--EWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYN 191

Query: 202 DFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTASNIKVAVHANVERIIVATSSASDNS 261
            F+ +H+ GTKIGG+ FD +G RH+AADLL Y    NI V +HA+V +I+  T       
Sbjct: 192 GFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKG---RP 251

Query: 262 KLSAIGVVYRDTMGHYHHAMVRGDG--EVILSAGAIGSPQLLLLSGIGSRPYLSSWGI-P 321
           +  A GV+++D  G  H A +  +   EVILSAGAIGSPQLL+LSGIG   +L++ GI P
Sbjct: 252 RPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKP 311

Query: 322 VSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVVGITDSGAYLEAASNVIPFGALSHS 381
           +    P VGQ + DNP N I I  P P+E SLIQVVGIT   +Y+E AS VI   + +  
Sbjct: 312 LVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRR 371

Query: 382 VF--------------SRSPTSPLYLT-------------------VATIMEKISGPVSS 441
            F              + S TSP   T                      I++KI+GP+S 
Sbjct: 372 FFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISR 431

Query: 442 GSLRLASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDILRSRSMEDFKFTWAGGRDFR 501
           G L L +T+   NPS+RFNY+ +P+DL +C+ G+  I  ++ S++   FK+  A      
Sbjct: 432 GHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLL 491

Query: 502 FLGPALPTDQSDDAL-----MGEFCRRTVSTIWHYHGGCVVGKVVDTDLRVTGVNALRVI 553
            L  ++PT+     +     + +FC  TV TIWHYHGGC VG+VVD + RV G+++LRVI
Sbjct: 492 DLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVI 551
[Close]
BLAST of Spo04534.1 vs. TAIR (Arabidopsis)
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 438.0 bits (1125), Expect = 8.900e-123
Identity = 241/534 (45.13%), Postives = 334/534 (62.55%), Query Frame = 1 

		  

Query: 57  YDYIIVGGGTAGCPLAATLSQNYKVLLLERGGVPYGNSNLMTQEGFLSTILEIDTFNSPA 116
           YDYI++GGGTAGCPLAATLSQN+ VL+LERGGVP+ N+N+     F   + +I   +S +
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLADISA-SSAS 122

Query: 117 QAFTSEEGVPNTRGRVLGGSSVINAGFYSRADLEFYAKSGLSWDLRMVNQSYEWVENAIV 176
           QAF S +GV N R RVLGG S INAGFYSRAD  F  ++G  WD ++V +SY WVE  IV
Sbjct: 123 QAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVEREIV 182

Query: 177 FRPDLRSWQSSVRDGLLEAGVSPYNDFSLEHVLGTKIGGSTFDSSGRRHSAADLLSYGTA 236
            +P L  WQ ++RD LLE GV P+N F+ +HV GTKIGG+ FD  GRRH+AA+LL+Y   
Sbjct: 183 HQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANP 242

Query: 237 SNIKVAVHANVERIIVATSSASDNSKLSAIGVVYRDTMGHYHHAMV--RGDGEVILSAGA 296
             ++V ++A V++I+  TS     ++    GV+++D  G+ H A++  R   EVILS+GA
Sbjct: 243 QKLRVLIYATVQKIVFDTS----GTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGA 302

Query: 297 IGSPQLLLLSGIGSRPYLSSWGIPVSHHSPYVGQFLYDNPRNGISIVPPMPLEHSLIQVV 356
           IGSPQ+L+LSGIG +  L    IPV   + +VG+ + DNP N I +    P+E SLIQ V
Sbjct: 303 IGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTV 362

Query: 357 GITDSGAYLEAASNV--------IPFGALS--HSVFSRSP-------TSPLYLT------ 416
           GIT  G Y+EA++            +G +S  + +FS  P        +  Y+T      
Sbjct: 363 GITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQL 422

Query: 417 -----VATIMEKISGPVSSGSLRLASTDVRLNPSIRFNYFSDPKDLDLCIVGMRKIGDIL 476
                 + I+EK++ P+S G L L +T+V  NPS+ FNYF  P DL  C+  +R +  ++
Sbjct: 423 HEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVV 482

Query: 477 RSRSMEDFKFTWAGGRD-FRFLGPAL-------PTDQSDDALMGEFCRRTVSTIWHYHGG 536
            S       +T    ++  + L  ++       P   +D   M +FC+ TV TIWHYHGG
Sbjct: 483 TSNRF--LNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGG 542

Query: 537 CVVGKVVDTDLRVTGVNALRVIDGSILTVSPGTNPQATLLMLGRYMGLKMLQER 553
           C+VGKVV  + +V GV+ LRVIDGS    SPGTNPQAT++M+GRYMG+K+L+ER
Sbjct: 543 CLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587
The following BLAST results are available for this feature:
BLAST of Spo04534.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902193537|gb|KNA12319.1|0.0e+0100.hypothetical protein SOVF_1267... [more]
gi|731351926|ref|XP_010687278.1|1.2e-28189.3PREDICTED: (R)-mandelonitrile ... [more]
gi|661897800|emb|CDO97767.1|1.5e-23372.8unnamed protein product [Coffe... [more]
gi|697120115|ref|XP_009614027.1|1.5e-23374.1PREDICTED: (R)-mandelonitrile ... [more]
gi|225427155|ref|XP_002277531.1|4.9e-23272.2PREDICTED: (R)-mandelonitrile ... [more]
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BLAST of Spo04534.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QYH0_SPIOL0.0e+0100.Uncharacterized protein OS=Spi... [more]
A0A0J8BV03_BETVU8.1e-28289.3Uncharacterized protein OS=Bet... [more]
A0A068TP29_COFCA1.1e-23372.8Uncharacterized protein OS=Cof... [more]
V4S0T4_9ROSI1.9e-23070.0Uncharacterized protein OS=Cit... [more]
A0A067DRK1_CITSI3.2e-23070.0Uncharacterized protein OS=Cit... [more]
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BLAST of Spo04534.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
MDLL_ARATH1.9e-22068.2(R)-mandelonitrile lyase-like ... [more]
MDL1_PRUSE1.3e-14749.8(R)-mandelonitrile lyase 1 OS=... [more]
MDL2_PRUDU1.7e-14749.8(R)-mandelonitrile lyase 2 OS=... [more]
MDL3_PRUSE1.1e-14650.0(R)-mandelonitrile lyase 3 OS=... [more]
MDL2_PRUSE1.4e-14650.9(R)-mandelonitrile lyase 2 OS=... [more]
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BLAST of Spo04534.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT1G73050.11.1e-22168.2Glucose-methanol-choline (GMC)... [more]
AT1G12570.12.4e-12844.4Glucose-methanol-choline (GMC)... [more]
AT3G56060.18.6e-12646.0Glucose-methanol-choline (GMC)... [more]
AT5G51950.11.9e-12544.5Glucose-methanol-choline (GMC)... [more]
AT1G72970.18.9e-12345.1Glucose-methanol-choline (GMC)... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 57..334
score: 3.5
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 130..153
scor
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 293..307
scor
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 402..542
score: 4.8
IPR023753FAD/NAD(P)-binding domainGENE3D3.50.50.60coord: 52..176
score: 7.0E-88coord: 493..552
score: 7.0E-88coord: 222..341
score: 7.0E-88coord: 412..426
score: 7.0
IPR023753FAD/NAD(P)-binding domainunknownSSF51905FAD/NAD(P)-binding domaincoord: 52..355
score: 3.45
NoneNo IPR availablePANTHERPTHR11552GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASEcoord: 33..554
score:
NoneNo IPR availablePANTHERPTHR11552:SF82(R)-MANDELONITRILE LYASE-LIKEcoord: 33..554
score:
NoneNo IPR availablePROFILEPS51257PROKAR_LIPOPROTEINcoord: 1..30
score:
NoneNo IPR availableunknownSSF54373FAD-linked reductases, C-terminal domaincoord: 330..498
score: 4.71

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006066 alcohol metabolic process
biological_process GO:0006544 glycine metabolic process
biological_process GO:0000373 Group II intron splicing
biological_process GO:0010564 regulation of cell cycle process
biological_process GO:0006626 protein targeting to mitochondrion
biological_process GO:0048825 cotyledon development
biological_process GO:0051301 cell division
biological_process GO:0007033 vacuole organization
biological_process GO:0009651 response to salt stress
biological_process GO:0080141 regulation of jasmonic acid biosynthetic process
biological_process GO:0044237 cellular metabolic process
biological_process GO:0034765 regulation of ion transmembrane transport
biological_process GO:0009556 microsporogenesis
biological_process GO:0061025 membrane fusion
biological_process GO:0086010 membrane depolarization during action potential
biological_process GO:0007030 Golgi organization
biological_process GO:0006952 defense response
biological_process GO:0052543 callose deposition in cell wall
biological_process GO:0019722 calcium-mediated signaling
biological_process GO:0009409 response to cold
biological_process GO:0006260 DNA replication
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009220 pyrimidine ribonucleotide biosynthetic process
biological_process GO:0006412 translation
biological_process GO:0042254 ribosome biogenesis
biological_process GO:0019430 removal of superoxide radicals
biological_process GO:0006810 transport
biological_process GO:0006364 rRNA processing
biological_process GO:0006397 mRNA processing
biological_process GO:0009553 embryo sac development
biological_process GO:0001510 RNA methylation
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0006177 GMP biosynthetic process
biological_process GO:0006541 glutamine metabolic process
biological_process GO:0006536 glutamate metabolic process
biological_process GO:0006531 aspartate metabolic process
biological_process GO:0006529 asparagine biosynthetic process
biological_process GO:0006522 alanine metabolic process
biological_process GO:0006351 transcription, DNA-templated
biological_process GO:0009165 nucleotide biosynthetic process
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0010119 regulation of stomatal movement
biological_process GO:0006606 protein import into nucleus
biological_process GO:0007267 cell-cell signaling
biological_process GO:0007346 regulation of mitotic cell cycle
biological_process GO:0009909 regulation of flower development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0010267 production of ta-siRNAs involved in RNA interference
biological_process GO:0035196 production of miRNAs involved in gene silencing by miRNA
biological_process GO:0009640 photomorphogenesis
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006312 mitotic recombination
biological_process GO:0009845 seed germination
biological_process GO:0000226 microtubule cytoskeleton organization
biological_process GO:0009933 meristem structural organization
biological_process GO:0051321 meiotic cell cycle
biological_process GO:0019915 lipid storage
biological_process GO:0009560 embryo sac egg cell differentiation
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0006302 double-strand break repair
biological_process GO:0010162 seed dormancy process
biological_process GO:0050826 response to freezing
biological_process GO:0000911 cytokinesis by cell plate formation
biological_process GO:0009616 virus induced gene silencing
biological_process GO:0006563 L-serine metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008152 metabolic process
biological_process GO:0010182 sugar mediated signaling pathway
biological_process GO:0016233 telomere capping
biological_process GO:0006566 threonine metabolic process
biological_process GO:0010228 vegetative to reproductive phase transition of meristem
biological_process GO:0010048 vernalization response
cellular_component GO:0030870 Mre11 complex
cellular_component GO:0042575 DNA polymerase complex
cellular_component GO:0009534 chloroplast thylakoid
cellular_component GO:0042644 chloroplast nucleoid
cellular_component GO:0000781 chromosome, telomeric region
cellular_component GO:0005840 ribosome
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0000325 plant-type vacuole
cellular_component GO:0005618 cell wall
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0005891 voltage-gated calcium channel complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005829 cytosol
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005730 nucleolus
cellular_component GO:0032991 protein-containing complex
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016462 pyrophosphatase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008812 choline dehydrogenase activity
molecular_function GO:0004784 superoxide dismutase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0046593 mandelonitrile lyase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016740 transferase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003922 GMP synthase (glutamine-hydrolyzing) activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0005515 protein binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
molecular_function GO:0004386 helicase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0004518 nuclease activity
molecular_function GO:0016887 ATPase activity
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005245 voltage-gated calcium channel activity
molecular_function GO:0008026 ATP-dependent helicase activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0005488 binding
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0003735 structural constituent of ribosome
RNA-Seq Expression
   



Co-expression
Gener valueExpression
Spo061680.75Barchart | Table
Spo092740.75Barchart | Table
Spo077940.74Barchart | Table
Spo097320.73Barchart | Table
Spo117560.72Barchart | Table
Spo195000.71Barchart | Table
Spo183040.71Barchart | Table
Spo190070.71Barchart | Table
Spo024720.71Barchart | Table
Spo256150.70Barchart | Table
Spo107050.70Barchart | Table
Spo239310.70Barchart | Table
Spo216190.70Barchart | Table
Spo044530.70Barchart | Table
Spo256720.70Barchart | Table
Spo051900.70Barchart | Table
Spo211960.70Barchart | Table
Spo208060.70Barchart | Table
Spo184790.70Barchart | Table
Spo184340.70Barchart | Table
Spo264520.70Barchart | Table
Spo256710.70Barchart | Table
Spo161060.70Barchart | Table
Spo153630.70Barchart | Table
Spo194990.69Barchart | Table
Spo123910.69Barchart | Table
Spo148230.69Barchart | Table
Spo154070.69Barchart | Table
Spo068810.69Barchart | Table
Spo242230.69Barchart | Table
Spo271000.69Barchart | Table
Spo235130.69Barchart | Table
Spo036270.69Barchart | Table
Spo216460.69Barchart | Table
Spo110520.69Barchart | Table
Spo168740.69Barchart | Table
Spo107980.69Barchart | Table
Spo106240.69Barchart | Table
Spo094930.69Barchart | Table
Spo090270.69Barchart | Table
Spo030590.69Barchart | Table
Spo118700.69Barchart | Table
Spo000810.68Barchart | Table
Spo007020.68Barchart | Table
Spo010960.68Barchart | Table
Spo021810.68Barchart | Table
Spo045310.68Barchart | Table
Spo085020.68Barchart | Table
Spo085990.68Barchart | Table
Spo090280.68Barchart | Table
Spo110930.68Barchart | Table
Spo121960.68Barchart | Table
Spo126100.68Barchart | Table
Spo130500.68Barchart | Table
Spo133870.68Barchart | Table
Spo151340.68Barchart | Table
Spo167730.68Barchart | Table
Spo171240.68Barchart | Table
Spo176950.68Barchart | Table
Spo197610.68Barchart | Table
Spo223890.68Barchart | Table
Spo225250.68Barchart | Table
Spo226610.68Barchart | Table
Spo231270.68Barchart | Table
Spo251230.68Barchart | Table
Spo253440.68Barchart | Table
Spo266800.68Barchart | Table
Spo271440.68Barchart | Table
Spo271520.68Barchart | Table
Spo121430.67Barchart | Table
Spo169630.67Barchart | Table
Spo032120.67Barchart | Table
Spo260080.67Barchart | Table
Spo170530.67Barchart | Table
Spo170810.67Barchart | Table
Spo202950.67Barchart | Table
Spo236300.67Barchart | Table
Spo061370.67Barchart | Table
Spo300250.67Barchart | Table
Spo115960.67Barchart | Table
Spo215310.67Barchart | Table
Spo039640.67Barchart | Table
Spo173050.67Barchart | Table
Spo219100.67Barchart | Table
Spo010010.67Barchart | Table
Spo177210.67Barchart | Table
Spo266320.67Barchart | Table
Spo087610.67Barchart | Table
Spo182000.67Barchart | Table
Spo268510.67Barchart | Table
Spo183930.67Barchart | Table
Spo068510.67Barchart | Table
Spo001550.67Barchart | Table
Spo189420.67Barchart | Table
Spo167940.66Barchart | Table
Spo090930.66Barchart | Table
Spo154310.66Barchart | Table
Spo167100.66Barchart | Table
Spo093530.66Barchart | Table
Spo168170.66Barchart | Table
Spo081050.66Barchart | Table
Spo011710.66Barchart | Table
Spo180700.66Barchart | Table
Spo070100.66Barchart | Table
Spo064550.66Barchart | Table
Spo062870.66Barchart | Table
Spo197760.66Barchart | Table
Spo199810.66Barchart | Table
Spo057320.66Barchart | Table
Spo039140.66Barchart | Table
Spo219390.66Barchart | Table
Spo262490.66Barchart | Table
Spo227120.66Barchart | Table
Spo228040.66Barchart | Table
Spo032140.66Barchart | Table
Spo239060.66Barchart | Table
Spo118820.66Barchart | Table
Spo120150.66Barchart | Table
Spo122910.66Barchart | Table
Spo132040.66Barchart | Table
Spo135090.66Barchart | Table
Spo137070.66Barchart | Table
Spo141590.66Barchart | Table
Spo143100.66Barchart | Table
Spo149740.66Barchart | Table
Spo154010.65Barchart | Table
Spo007000.65Barchart | Table
Spo224750.65Barchart | Table
Spo020320.65Barchart | Table
Spo244400.65Barchart | Table
Spo211040.65Barchart | Table
Spo244750.65Barchart | Table
Spo206120.65Barchart | Table
Spo140130.65Barchart | Table
Spo061720.65Barchart | Table
Spo165680.65Barchart | Table
Spo105840.65Barchart | Table
Spo063990.65Barchart | Table
Spo031050.65Barchart | Table