Spo02535 (gene) | SpinachBase

Overview


Name	Spo02535
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Isochorismatase hydrolase family protein
Location	chr3 : 6593132 .. 6593658 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAAAATGGAAGAAGACAGCTCTCTTTGTCATAGATATGCAAGTAAGTTCATGCTTCATTGCTTCAATTCCCATTTTTTTTCACGCCATCCCTTCTCTTCTTGTTTAGAATTCAAACTGGGTTTTTTCTCTCCTTAAAAAATGGGTAGTAAATTAATAAATTTTAATGTTGAATGATCATCAACATTTATAATTGAAGTTGGGTTTGATTCGAATTGCAGAAAGATTTCGTGTTGCCGGGTGCGCCGTTGCTGGTGGATGGTGCTGCTGCTATTGTTCCTGATGTTATCAAAGCTGTTCAAGTTGCAAGAGAGCGTGGCATGCTTGTTGTTTGGGTATTATTCAACCCTTTTTTTTAAAAAATTATGCAGAGTTTTAGCAATTGTTGTTGATTTGCTAGTGTTATAAATTAGTGATTTCATATGTTATTGTGGTAATTTGTTTGTAAAGTTGCTTGTGAAATTGAGAAAAATTGAGCAGAAAAGAGATGATGCTACTGATAGTCAAACATTATTTGGGTAA

mRNA sequence

ATGGCTGAAAAATGGAAGAAGACAGCTCTCTTTGTCATAGATATGCAAAAAGATTTCGTGTTGCCGGGTGCGCCGTTGCTGGTGGATGGTGCTGCTGCTATTGTTCCTGATGTTATCAAAGCTGTTCAAGTTGCAAGAGAGCGTGGCATGCTTGTTGTTTGGTTGCTTGTGAAATTGAGAAAAATTGAGCAGAAAAGAGATGATGCTACTGATAGTCAAACATTATTTGGGTAA

Coding sequence (CDS)

ATGGCTGAAAAATGGAAGAAGACAGCTCTCTTTGTCATAGATATGCAAAAAGATTTCGTGTTGCCGGGTGCGCCGTTGCTGGTGGATGGTGCTGCTGCTATTGTTCCTGATGTTATCAAAGCTGTTCAAGTTGCAAGAGAGCGTGGCATGCTTGTTGTTTGGTTGCTTGTGAAATTGAGAAAAATTGAGCAGAAAAGAGATGATGCTACTGATAGTCAAACATTATTTGGGTAA

Protein sequence

MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLLVKLRKIEQKRDDATDSQTLFG

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo02535.1	Spo02535.1	mRNA

Homology

[Close]

BLAST of Spo02535.1 vs. NCBI nr
Match: gi|902194714|gb|KNA12635.1| (hypothetical protein SOVF_124260, partial [Spinacia oleracea])

HSP 1 Score: 107.8 bits (268), Expect = 8.300e-21
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVW 55
          MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVW
Sbjct: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVW 54

[Close]

BLAST of Spo02535.1 vs. NCBI nr
Match: gi|902231917|gb|KNA22449.1| (hypothetical protein SOVF_033290 [Spinacia oleracea])

HSP 1 Score: 99.0 bits (245), Expect = 3.900e-18
Identity = 48/56 (85.71%), Postives = 54/56 (96.43%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          MA+ WKKTAL VIDMQKDFVLPGAP+LVDGAAAIVP+VIKAV+VARERGMLVVW++
Sbjct: 1  MAQTWKKTALLVIDMQKDFVLPGAPMLVDGAAAIVPNVIKAVEVARERGMLVVWVV 56

[Close]

BLAST of Spo02535.1 vs. NCBI nr
Match: gi|731359614|ref|XP_010691375.1| (PREDICTED: uncharacterized protein LOC104904714 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 96.3 bits (238), Expect = 2.500e-17
Identity = 45/56 (80.36%), Postives = 52/56 (92.86%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          M +KWKKTALFVIDMQKDF+LP  PLLVDG AAI+P+VIKAV+VARERGMLVVW++
Sbjct: 1  MTDKWKKTALFVIDMQKDFILPDGPLLVDGGAAIIPNVIKAVEVARERGMLVVWVV 56

[Close]

BLAST of Spo02535.1 vs. NCBI nr
Match: gi|970013915|ref|XP_015067938.1| (PREDICTED: probable inactive nicotinamidase At3g16190 isoform X3 [Solanum pennellii])

HSP 1 Score: 79.0 bits (193), Expect = 4.100e-12
Identity = 35/54 (64.81%), Postives = 46/54 (85.19%), Query Frame = 1


		  
Query: 3  EKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          +KW KTA+ VIDMQKDF+LPGAP+LV G  AIVP+VIK V+VAR RG+ ++W++
Sbjct: 7  DKWSKTAVVVIDMQKDFILPGAPMLVRGGEAIVPNVIKTVEVARNRGIPIIWVV 60

[Close]

BLAST of Spo02535.1 vs. NCBI nr
Match: gi|595943850|ref|XP_007215992.1| (hypothetical protein PRUPE_ppa011798mg [Prunus persica])

HSP 1 Score: 79.0 bits (193), Expect = 4.100e-12
Identity = 39/56 (69.64%), Postives = 45/56 (80.36%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          MA KWK TAL VIDMQ DFV    P+LV G  AIVP+VIKAVQ+AR+RGMLVVW++
Sbjct: 1  MANKWKHTALLVIDMQHDFVDVDGPMLVSGGKAIVPNVIKAVQIARQRGMLVVWVV 56

[Close]

BLAST of Spo02535.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QZF7_SPIOL (Uncharacterized protein (Fragment) OS=Spinacia oleracea GN=SOVF_124260 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 5.800e-21
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVW 55
          MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVW
Sbjct: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVW 54

[Close]

BLAST of Spo02535.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RU53_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_033290 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 2.700e-18
Identity = 48/56 (85.71%), Postives = 54/56 (96.43%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          MA+ WKKTAL VIDMQKDFVLPGAP+LVDGAAAIVP+VIKAV+VARERGMLVVW++
Sbjct: 1  MAQTWKKTALLVIDMQKDFVLPGAPMLVDGAAAIVPNVIKAVEVARERGMLVVWVV 56

[Close]

BLAST of Spo02535.1 vs. UniProtKB/TrEMBL
Match: A0A0J8ECW9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g221300 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.700e-17
Identity = 45/56 (80.36%), Postives = 52/56 (92.86%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          M +KWKKTALFVIDMQKDF+LP  PLLVDG AAI+P+VIKAV+VARERGMLVVW++
Sbjct: 1  MTDKWKKTALFVIDMQKDFILPDGPLLVDGGAAIIPNVIKAVEVARERGMLVVWVV 56

[Close]

BLAST of Spo02535.1 vs. UniProtKB/TrEMBL
Match: M5WVC8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011798mg PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.900e-12
Identity = 39/56 (69.64%), Postives = 45/56 (80.36%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          MA KWK TAL VIDMQ DFV    P+LV G  AIVP+VIKAVQ+AR+RGMLVVW++
Sbjct: 1  MANKWKHTALLVIDMQHDFVDVDGPMLVSGGKAIVPNVIKAVQIARQRGMLVVWVV 56

[Close]

BLAST of Spo02535.1 vs. UniProtKB/TrEMBL
Match: A0A068TP28_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00021975001 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.900e-12
Identity = 35/53 (66.04%), Postives = 45/53 (84.91%), Query Frame = 1


		  
Query: 4  KWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          KWKKTAL VIDMQ DF+LPG P+LV G  AIVP+VI+AV+VAR RG+ ++W++
Sbjct: 7  KWKKTALLVIDMQNDFILPGGPMLVKGGQAIVPNVIRAVEVARGRGIPIIWVV 59

[Close]

BLAST of Spo02535.1 vs. ExPASy Swiss-Prot
Match: NIC4_ARATH (Probable inactive nicotinamidase At3g16190 OS=Arabidopsis thaliana GN=At3g16190 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.700e-11
Identity = 31/56 (55.36%), Postives = 44/56 (78.57%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          MAE+W+ TAL VIDMQ DF+  GA   V G  +IVP+VI+ V++AR+RG+LV+W++
Sbjct: 1  MAERWRNTALLVIDMQNDFIEEGAVTQVKGGKSIVPNVIRVVELARQRGILVIWVV 56

[Close]

BLAST of Spo02535.1 vs. TAIR (Arabidopsis)
Match: AT3G16190.1 (Isochorismatase family protein)

HSP 1 Score: 68.9 bits (167), Expect = 1.500e-12
Identity = 31/56 (55.36%), Postives = 44/56 (78.57%), Query Frame = 1


		  
Query: 1  MAEKWKKTALFVIDMQKDFVLPGAPLLVDGAAAIVPDVIKAVQVARERGMLVVWLL 57
          MAE+W+ TAL VIDMQ DF+  GA   V G  +IVP+VI+ V++AR+RG+LV+W++
Sbjct: 1  MAERWRNTALLVIDMQNDFIEEGAVTQVKGGKSIVPNVIRVVELARQRGILVIWVV 56

The following BLAST results are available for this feature:

BLAST of Spo02535.1 vs. NCBI nr
BLAST of Spo02535.1 vs. UniProtKB/TrEMBL
BLAST of Spo02535.1 vs. ExPASy Swiss-Prot
BLAST of Spo02535.1 vs. TAIR (Arabidopsis)

BLAST of Spo02535.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902194714\|gb\|KNA12635.1\|	8.3e-21	100.	hypothetical protein SOVF_1242...	[more]
gi\|902231917\|gb\|KNA22449.1\|	3.9e-18	85.7	hypothetical protein SOVF_0332...	[more]
gi\|731359614\|ref\|XP_010691375.1\|	2.5e-17	80.3	PREDICTED: uncharacterized pro...	[more]
gi\|970013915\|ref\|XP_015067938.1\|	4.1e-12	64.8	PREDICTED: probable inactive n...	[more]
gi\|595943850\|ref\|XP_007215992.1\|	4.1e-12	69.6	hypothetical protein PRUPE_ppa...	[more]

BLAST of Spo02535.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QZF7_SPIOL	5.8e-21	100.	Uncharacterized protein (Fragm...	[more]
A0A0K9RU53_SPIOL	2.7e-18	85.7	Uncharacterized protein OS=Spi...	[more]
A0A0J8ECW9_BETVU	1.7e-17	80.3	Uncharacterized protein OS=Bet...	[more]
M5WVC8_PRUPE	2.9e-12	69.6	Uncharacterized protein OS=Pru...	[more]
A0A068TP28_COFCA	4.9e-12	66.0	Uncharacterized protein OS=Cof...	[more]

BLAST of Spo02535.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1


Match Name	E-value	Identity	Description
NIC4_ARATH	2.7e-11	55.3	Probable inactive nicotinamida...	[more]

BLAST of Spo02535.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G16190.1	1.5e-12	55.3	Isochorismatase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000868	Isochorismatase-like	GENE3D	3.40.50.850		coord: 5..57 score: 1.1
IPR000868	Isochorismatase-like	PFAM	PF00857	Isochorismatase	coord: 8..60 score: 8.
IPR000868	Isochorismatase-like	unknown	SSF52499	Isochorismatase-like hydrolases	coord: 5..57 score: 1.57

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0016049	cell growth
biological_process	GO:0006623	protein targeting to vacuole
biological_process	GO:0006486	protein glycosylation
biological_process	GO:0010228	vegetative to reproductive phase transition of meristem
biological_process	GO:0007033	vacuole organization
biological_process	GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0048193	Golgi vesicle transport
biological_process	GO:0000902	cell morphogenesis
biological_process	GO:0007030	Golgi organization
biological_process	GO:0006470	protein dephosphorylation
biological_process	GO:0009767	photosynthetic electron transport chain
biological_process	GO:0080005	photosystem stoichiometry adjustment
cellular_component	GO:0005829	cytosol
cellular_component	GO:0009579	thylakoid
cellular_component	GO:0008287	protein serine/threonine phosphatase complex
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0004722	protein serine/threonine phosphatase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0005515	protein binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo06949	0.91	Barchart \| Table
Spo23527	0.72	Barchart \| Table
Spo02536	0.72	Barchart \| Table
Spo27102	0.70	Barchart \| Table
Spo11951	0.70	Barchart \| Table
Spo13029	0.69	Barchart \| Table
Spo04104	0.69	Barchart \| Table
Spo02484	0.69	Barchart \| Table
Spo02513	0.69	Barchart \| Table
Spo23588	0.68	Barchart \| Table
Spo00812	0.68	Barchart \| Table
Spo02747	0.68	Barchart \| Table
Spo04908	0.68	Barchart \| Table
Spo08969	0.68	Barchart \| Table
Spo11346	0.68	Barchart \| Table
Spo15630	0.68	Barchart \| Table
Spo19874	0.68	Barchart \| Table
Spo05093	0.67	Barchart \| Table
Spo24857	0.67	Barchart \| Table
Spo10473	0.67	Barchart \| Table
Spo12107	0.67	Barchart \| Table
Spo14454	0.67	Barchart \| Table
Spo16652	0.67	Barchart \| Table
Spo01261	0.67	Barchart \| Table
Spo22760	0.67	Barchart \| Table
Spo01397	0.67	Barchart \| Table
Spo23935	0.67	Barchart \| Table
Spo06729	0.67	Barchart \| Table
Spo08680	0.67	Barchart \| Table
Spo06146	0.66	Barchart \| Table
Spo18735	0.66	Barchart \| Table
Spo26427	0.66	Barchart \| Table
Spo01840	0.66	Barchart \| Table
Spo14403	0.66	Barchart \| Table
Spo15275	0.66	Barchart \| Table
Spo26811	0.66	Barchart \| Table
Spo10316	0.66	Barchart \| Table
Spo00715	0.65	Barchart \| Table
Spo22529	0.65	Barchart \| Table
Spo20497	0.65	Barchart \| Table
Spo06679	0.65	Barchart \| Table
Spo04483	0.65	Barchart \| Table